Carg27016 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg27016
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Membrane-associated kinase regulator, putative
Location	Carg_Chr15: 7560124 .. 7561243 (+)
RNA-Seq Expression	Carg27016
Synteny	Carg27016

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACTAAAACAAGAACAAGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTTGAGCTTTTGTCTGTTTCCGCCGGCGGAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGATCAAATTAGGCGAACATATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTGCCAATCGAGACCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCACTGGCACAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCATGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCCGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCGGATTGGCGGCGGCAGCGGTGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTAGAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGGTAAAAACAAAGAAAAGAAACCTCTTTTAAACTTATAATTAGGCCATTGATTTTTGAATTATGTTTTTGTGGTCAGAGAAACCTGTGTTGGGTGTGGACGTCTGAGAGAGGTATTTCAGAGTATTATGTTCTTGTTCGATTCTATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAAAACAGTGAAAAAGAAGAGAAATTGTTGGGTGATGGAAGTGATGATGAAACAACACAAATGGGGTTCAAGTTTATTTCTTTTAAAATCTTGCCTTCAAC

mRNA sequence

ATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACTAAAACAAGAACAAGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTTGAGCTTTTGTCTGTTTCCGCCGGCGGAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGATCAAATTAGGCGAACATATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTGCCAATCGAGACCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCACTGGCACAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCATGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCCGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCGGATTGGCGGCGGCAGCGGTGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTAGAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGAGAAACCTGTGTTGGGTGTGGACGTCTGAGAGAGGTATTTCAGAGTATTATGTTCTTGTTCGATTCTATTTTCCGCCATTGATTTGGAAGCAAAGAGCTTCAAAATAATAAAATCTGAGAGAAACCCAGAAGAGGAAAAACAGTGAAAAAGAAGAGAAATTGTTGGGTGATGGAAGTGATGATGAAACAACACAAATGGGGTTCAAGTTTATTTCTTTTAAAATCTTGCCTTCAAC

Coding sequence (CDS)

ATGGATTCCGTCGCCTATCTCAAGTTCTTCAAATTTTCCGGCGCCGATGGACTACTAAAACAAGAACAAGAGGAGGAAGAAGAAGAAGAAGAAGAAGAAGAAGATTCGTTCTTCGATCTTGAGCTTTTGTCTGTTTCCGCCGGCGGAATTCAAAGCCCAGAACAGAGGCCTCAAATTCCGATCAAATTAGGCGAACATATGAACCTCTGTTCCGATTCCATTTCGAAACGGAAGATTCTGCCAATCGAGACCACTTCGAAGCCCCAATCTCCGATTCAATTTCTCAAATCAGCTCCCAAATTCACTGATTTCATCTTCAAGAAACAGAGATCAATGGCCAAGAACAGAGCACCCACTGGCACAGAACAAGAAGAAACCCGAATTCTAAGCTCGAAATTCACCAGAGACAACAGCACAAGAAGAATCAGAGGCATTTTTCCACCGGACCCATGTGGGGTTCCGGCTCCATCTCCGGCATGGCGGTTCTCGAAGGAGGCGGTGCAGAAATATCTAAAGCTGATAAAGCCTAAGCTTCTGAAGAAACCCACCGACAGCGGCGGCGAGTTGTCGTCCGCAGCTGTGGATTCTCCGGTGAGGGAGAAGCTCGAGAATGGTCTTCCGACAGGGATTCGGTTGGTCCGTAAGCATCTGGGTAGGAGCAAATCGGCCTCGGCGGCTAGCGGATTGGCGGCGGCAGCGGTGGCGGTGGCTCCTCCGCCGAACCGGAGGGATGATTCGTTATTAGAGCAACACGACGGGATTGAAAGCGCCATTTTACACTGTAAAAGATCCTTGAATGGATCCACAGAGAAACCTGTGTTGGGTGTGGACGTCTGA

Protein sequence

MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV

Homology

[Close]

BLAST of Carg27016 vs. NCBI nr
Match: KAG7016845.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 539.3 bits (1388), Expect = 2.0e-149
Identity = 278/278 (100.00%), Postives = 278/278 (100.00%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
           IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG
Sbjct: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
           TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 278

[Close]

BLAST of Carg27016 vs. NCBI nr
Match: KAG6579360.1 (putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 522.7 bits (1345), Expect = 1.9e-144
Identity = 276/287 (96.17%), Postives = 276/287 (96.17%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQ---EEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRP 60
           MDSVAYLKFFKFSGADGLLKQEQ   EEEEEEEEEEEDSFFDLEL SVSAGGIQSPEQRP
Sbjct: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEEEEDSFFDLELFSVSAGGIQSPEQRP 60

Query: 61  QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120
           QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA
Sbjct: 61  QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120

Query: 121 PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK
Sbjct: 121 PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180

Query: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSAS------AASGLAA 240
           LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSAS      AASGLAA
Sbjct: 181 LLKKPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLAAASGLAA 240

Query: 241 AAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           AAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 AAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 287

[Close]

BLAST of Carg27016 vs. NCBI nr
Match: XP_022922154.1 (probable membrane-associated kinase regulator 5 [Cucurbita moschata])

HSP 1 Score: 506.9 bits (1304), Expect = 1.1e-139
Identity = 263/278 (94.60%), Postives = 266/278 (95.68%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKF+GADGLLKQEQ   EEEEEEEEDS FDLEL SVSAGGIQSPEQRPQIP
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQ---EEEEEEEEDSLFDLELFSVSAGGIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRS AKNRAP G
Sbjct: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
           TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTD+G ELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASA SGLAAAAVAVAPPP
Sbjct: 181 KPTDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLL+QHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 NRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

[Close]

BLAST of Carg27016 vs. NCBI nr
Match: XP_023550940.1 (probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 493.0 bits (1268), Expect = 1.6e-135
Identity = 260/278 (93.53%), Postives = 262/278 (94.24%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKFSGADGL KQEQ EEEEEEEEEEDSFFDLEL SVSAG IQSPEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLRKQEQ-EEEEEEEEEEDSFFDLELFSVSAGKIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLGEHMNLCSDSISKRKILPIE T KPQS IQFLKSAPKFTDFIFKKQRSM+KNRAP G
Sbjct: 61  TKLGEHMNLCSDSISKRKILPIEHTLKPQSSIQFLKSAPKFTDFIFKKQRSMSKNRAPAG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            EQEETRILSSKFTRDNS RRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 PEQEETRILSSKFTRDNSIRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASAASGLA    AVAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASGLA----AVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLL+QHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 NRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 273

[Close]

BLAST of Carg27016 vs. NCBI nr
Match: XP_022973059.1 (probable membrane-associated kinase regulator 5 [Cucurbita maxima])

HSP 1 Score: 488.4 bits (1256), Expect = 4.0e-134
Identity = 259/278 (93.17%), Postives = 262/278 (94.24%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKFSGADGL KQEQ  +EEEEEEEEDSFFDLELLSVSAG IQ PEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQ--DEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAP G
Sbjct: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            EQEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASAAS L AAAVAVAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-AAAVAVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLL+ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 NRRDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

[Close]

BLAST of Carg27016 vs. ExPASy Swiss-Prot
Match: Q9FLX4 (Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 8.2e-21
Identity = 85/248 (34.27%), Postives = 125/248 (50.40%), Query Frame = 0


Query: 32  EEEDSFFDLELLSVSAGGIQSPEQRPQIPIKLGEHMNLCSDSIS-KRKILP-IETTSKP- 91
           E EDSFF+L+   +S    ++P+++     +L       + S+S K K+LP ++ +SKP 
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQ----RLETTTTTTTYSVSNKSKVLPFVDISSKPQ 104

Query: 92  QSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTGTEQEETRI-LSSKFTRDNSTRRIRGIFP 151
           QSP   LKS  KF  F FKK     K+      ++E  R  L+ +F  D+ T        
Sbjct: 105 QSPTTLLKSGQKFRAFSFKKSE---KSTTTEKKKEENNRTSLNVRFRVDDET-TTTSFRK 164

Query: 152 PDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSP-VREKLENGL 211
                    +    F     +++  LIKP   K          ++   SP  REK  + +
Sbjct: 165 TASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQRSNI 224

Query: 212 PTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLN 271
           P+G+R VR+ LG+S+SASAA G  +       P NR D+SL  Q DGI+SAILHCK+S +
Sbjct: 225 PSGMRSVRRQLGKSRSASAAIGGMS-------PANRIDESLQVQQDGIQSAILHCKKSFH 274

Query: 272 GSTEKPVL 275
           GS E  +L
Sbjct: 285 GSRESSLL 274

[Close]

BLAST of Carg27016 vs. ExPASy Swiss-Prot
Match: Q9SH58 (Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 GN=MAKR2 PE=2 SV=1)

HSP 1 Score: 81.3 bits (199), Expect = 2.0e-14
Identity = 50/128 (39.06%), Postives = 69/128 (53.91%), Query Frame = 0


Query: 167 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 226
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 227 PTGIRLVRKHLGRSKSASAAS---GLAAAAVAVAPPPNRRDDSLLEQHDGIESAILHCKR 272
           P G+++VRKHLG+S+S+S+ +       A V       RRDDSLL+Q D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

[Close]

BLAST of Carg27016 vs. ExPASy TrEMBL
Match: A0A6J1E2K7 (probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN=LOC111430217 PE=4 SV=1)

HSP 1 Score: 506.9 bits (1304), Expect = 5.3e-140
Identity = 263/278 (94.60%), Postives = 266/278 (95.68%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKF+GADGLLKQEQ   EEEEEEEEDS FDLEL SVSAGGIQSPEQRPQIP
Sbjct: 1   MDSVAYLKFFKFAGADGLLKQEQ---EEEEEEEEDSLFDLELFSVSAGGIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRS AKNRAP G
Sbjct: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSTAKNRAPAG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
           TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTD+G ELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASA SGLAAAAVAVAPPP
Sbjct: 181 KPTDTGSELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASATSGLAAAAVAVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLL+QHDGIESAILHCKRSLNGSTEKPVLGVDV
Sbjct: 241 NRRDDSLLQQHDGIESAILHCKRSLNGSTEKPVLGVDV 275

[Close]

BLAST of Carg27016 vs. ExPASy TrEMBL
Match: A0A6J1IDF3 (probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=LOC111471559 PE=4 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 2.0e-134
Identity = 259/278 (93.17%), Postives = 262/278 (94.24%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVAYLKFFKFSGADGL KQEQ  +EEEEEEEEDSFFDLELLSVSAG IQ PEQRPQIP
Sbjct: 1   MDSVAYLKFFKFSGADGLPKQEQ--DEEEEEEEEDSFFDLELLSVSAGKIQGPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLGEHMNLCSDSISKRKILPIE TSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAP G
Sbjct: 61  TKLGEHMNLCSDSISKRKILPIEPTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPAG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            EQEETRILSSKFTRDNSTRRIRGIFPPDP GVPAPSPAWRFSKEAVQKYLKLIKPKLLK
Sbjct: 121 PEQEETRILSSKFTRDNSTRRIRGIFPPDPYGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTDSGGELSSAAVDSPVREKLENGLPTGIRLV KHLGRSKSASAAS L AAAVAVAPPP
Sbjct: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVCKHLGRSKSASAASRL-AAAVAVAPPP 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPVLGVDV 279
           NRRDDSLL+ HDGIESAILHCKRSLN STEKPVL +DV
Sbjct: 241 NRRDDSLLQLHDGIESAILHCKRSLNASTEKPVLAMDV 275

[Close]

BLAST of Carg27016 vs. ExPASy TrEMBL
Match: A0A1S3AUW4 (probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC103482925 PE=4 SV=1)

HSP 1 Score: 410.6 bits (1054), Expect = 5.2e-111
Identity = 216/276 (78.26%), Postives = 237/276 (85.87%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVA+LKFFK SGAD L KQE+EEEEEEEEEEEDSFFD+E  SVSAG IQSPEQRPQIP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLG+H+NLCSDSISKRKILPIE TSKPQSPIQ LKSAPKF  FIFKKQRSMAKN+APTG
Sbjct: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKAPTG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            E +++ + SSK TRDNSTRR  GIFP DPC  P P+P  RFSKEAV+KY KLIKPK+L+
Sbjct: 121 QEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPKVLR 180

Query: 181 KPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVA 240
           KPTDSG +LSS   AAVDSPVREK ENGLP GIRLV +HLG+SKSASAA GLAA   AV 
Sbjct: 181 KPTDSGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA---AVV 240

Query: 241 PPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPV 274
           PPPNRRDDSLL+QHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 PPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 273

[Close]

BLAST of Carg27016 vs. ExPASy TrEMBL
Match: A0A0A0KK03 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 1.2e-110
Identity = 217/273 (79.49%), Postives = 234/273 (85.71%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQEQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRPQIP 60
           MDSVA+LKFFK SGAD L KQE EEEEEEEEE EDSFFD+E  SVSAG IQSPEQRPQIP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQE-EEEEEEEEEGEDSFFDIEFFSVSAGKIQSPEQRPQIP 60

Query: 61  IKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTG 120
            KLG+H+NLCSDSISKRKILPIE TSKPQSPIQ LKSAPKF  FIFKKQRSMAKNR PTG
Sbjct: 61  TKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNRPPTG 120

Query: 121 TEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLK 180
            E  E+R+L+SK TRDNSTRR  GIFPPDPC  P P+P  RFSKE VQKYLKLIKPK+L+
Sbjct: 121 QEGGESRVLNSKLTRDNSTRRFGGIFPPDPCEPPVPAPTRRFSKEVVQKYLKLIKPKVLR 180

Query: 181 KPTDSGGELSSAAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPP 240
           KPTDSG +LSSAAVDSPVREK ENGLP GIRLV +HLG+SKSASAA GLAA   AV P  
Sbjct: 181 KPTDSGSDLSSAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA---AVIPQT 240

Query: 241 NRRDDSLLEQHDGIESAILHCKRSLNGSTEKPV 274
           NRRDDSLL+QHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 NRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 269

[Close]

BLAST of Carg27016 vs. ExPASy TrEMBL
Match: A0A5D3C3T3 (Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00860 PE=4 SV=1)

HSP 1 Score: 401.7 bits (1031), Expect = 2.4e-108
Identity = 214/279 (76.70%), Postives = 236/279 (84.59%), Query Frame = 0


Query: 1   MDSVAYLKFFKFSGADGLLKQ---EQEEEEEEEEEEEDSFFDLELLSVSAGGIQSPEQRP 60
           MDSVA+LKFFK SGAD L KQ   E+EEEEEEEEEEEDSFFD+E  SVSAG IQSPEQRP
Sbjct: 1   MDSVAFLKFFKHSGADELQKQEEEEEEEEEEEEEEEEDSFFDIEFFSVSAGKIQSPEQRP 60

Query: 61  QIPIKLGEHMNLCSDSISKRKILPIETTSKPQSPIQFLKSAPKFTDFIFKKQRSMAKNRA 120
           QIP KLG+H+NLCSDSISKRKILPIE TSKPQSPIQ LKSAPKF  FIFKKQRSMAKN+A
Sbjct: 61  QIPTKLGDHINLCSDSISKRKILPIEPTSKPQSPIQLLKSAPKFPVFIFKKQRSMAKNKA 120

Query: 121 PTGTEQEETRILSSKFTRDNSTRRIRGIFPPDPCGVPAPSPAWRFSKEAVQKYLKLIKPK 180
           PT  E +++ + SSK TRDNSTRR  GIFP DPC  P P+P  RFSKEAV+KY KLIKPK
Sbjct: 121 PTAQEGDQSGVPSSKLTRDNSTRRFGGIFPSDPCEPPVPAPTRRFSKEAVRKYFKLIKPK 180

Query: 181 LLKKPTDSGGELSS---AAVDSPVREKLENGLPTGIRLVRKHLGRSKSASAASGLAAAAV 240
           +L+KPTD+G +LSS   AAVDSPVREK ENGLP GIRLV +HLG+SKSASAA GLAA   
Sbjct: 181 VLRKPTDTGSDLSSSSAAAVDSPVREKHENGLPAGIRLVCRHLGKSKSASAAGGLAA--- 240

Query: 241 AVAPPPNRRDDSLLEQHDGIESAILHCKRSLNGSTEKPV 274
           AV PPPNRRDDSLL+QHDGI+SAILHCKRS N S+EKP+
Sbjct: 241 AVVPPPNRRDDSLLQQHDGIQSAILHCKRSFNASSEKPL 276

[Close]

BLAST of Carg27016 vs. TAIR 10
Match: AT5G52870.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G64080.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 102.4 bits (254), Expect = 5.8e-22
Identity = 85/248 (34.27%), Postives = 125/248 (50.40%), Query Frame = 0


Query: 32  EEEDSFFDLELLSVSAGGIQSPEQRPQIPIKLGEHMNLCSDSIS-KRKILP-IETTSKP- 91
           E EDSFF+L+   +S    ++P+++     +L       + S+S K K+LP ++ +SKP 
Sbjct: 45  EGEDSFFELK---ISLSDFKTPKEKQ----RLETTTTTTTYSVSNKSKVLPFVDISSKPQ 104

Query: 92  QSPIQFLKSAPKFTDFIFKKQRSMAKNRAPTGTEQEETRI-LSSKFTRDNSTRRIRGIFP 151
           QSP   LKS  KF  F FKK     K+      ++E  R  L+ +F  D+ T        
Sbjct: 105 QSPTTLLKSGQKFRAFSFKKSE---KSTTTEKKKEENNRTSLNVRFRVDDET-TTTSFRK 164

Query: 152 PDPCGVPAPSPAWRFSKEAVQKYLKLIKPKLLKKPTDSGGELSSAAVDSP-VREKLENGL 211
                    +    F     +++  LIKP   K          ++   SP  REK  + +
Sbjct: 165 TASIARSQQTDDTMFDDSVSKRFFSLIKPLYTKSTKKQSSSTITSPTSSPATREKQRSNI 224

Query: 212 PTGIRLVRKHLGRSKSASAASGLAAAAVAVAPPPNRRDDSLLEQHDGIESAILHCKRSLN 271
           P+G+R VR+ LG+S+SASAA G  +       P NR D+SL  Q DGI+SAILHCK+S +
Sbjct: 225 PSGMRSVRRQLGKSRSASAAIGGMS-------PANRIDESLQVQQDGIQSAILHCKKSFH 274

Query: 272 GSTEKPVL 275
           GS E  +L
Sbjct: 285 GSRESSLL 274

[Close]

BLAST of Carg27016 vs. TAIR 10
Match: AT1G64080.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G52870.1); Has 1546 Blast hits to 738 proteins in 176 species: Archae - 0; Bacteria - 143; Metazoa - 278; Fungi - 131; Plants - 95; Viruses - 4; Other Eukaryotes - 895 (source: NCBI BLink). )

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-15
Identity = 50/128 (39.06%), Postives = 69/128 (53.91%), Query Frame = 0


Query: 167 VQKYLKLIKPKLLKKPTDSGGEL--------------SSAAVDSPVREKLEN------GL 226
           +QKYLK +KP  ++     G +L              ++   DSPV  K  N       +
Sbjct: 267 MQKYLKKVKPLYIRVSRRYGEKLRHSGQLTAPSSARSTAEKADSPVATKTNNKPGNNINI 326

Query: 227 PTGIRLVRKHLGRSKSASAAS---GLAAAAVAVAPPPNRRDDSLLEQHDGIESAILHCKR 272
           P G+++VRKHLG+S+S+S+ +       A V       RRDDSLL+Q D I+SAILHCKR
Sbjct: 327 PAGLKVVRKHLGKSRSSSSTATTPSSTTATVTTQSESRRRDDSLLQQQDSIQSAILHCKR 386

The following BLAST results are available for this feature:

BLAST of Carg27016 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7016845.1	2.0e-149	100.00	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
KAG6579360.1	1.9e-144	96.17	putative membrane-associated kinase regulator 5, partial [Cucurbita argyrosperma...	[more]
XP_022922154.1	1.1e-139	94.60	probable membrane-associated kinase regulator 5 [Cucurbita moschata]	[more]
XP_023550940.1	1.6e-135	93.53	probable membrane-associated kinase regulator 5 [Cucurbita pepo subsp. pepo]	[more]
XP_022973059.1	4.0e-134	93.17	probable membrane-associated kinase regulator 5 [Cucurbita maxima]	[more]

BLAST of Carg27016 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FLX4	8.2e-21	34.27	Membrane-associated kinase regulator 5 OS=Arabidopsis thaliana OX=3702 GN=MAKR5 ...	[more]
Q9SH58	2.0e-14	39.06	Probable membrane-associated kinase regulator 2 OS=Arabidopsis thaliana OX=3702 ...	[more]

BLAST of Carg27016 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E2K7	5.3e-140	94.60	probable membrane-associated kinase regulator 5 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IDF3	2.0e-134	93.17	probable membrane-associated kinase regulator 5 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A1S3AUW4	5.2e-111	78.26	probable membrane-associated kinase regulator 5 OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KK03	1.2e-110	79.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G139900 PE=4 SV=1	[more]
A0A5D3C3T3	2.4e-108	76.70	Putative membrane-associated kinase regulator 5 OS=Cucumis melo var. makuwa OX=1...	[more]

BLAST of Carg27016 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G52870.1	5.8e-22	34.27	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G64080.1	1.4e-15	39.06	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 15..35
None	No IPR available	PANTHER	PTHR33929:SF4	MEMBRANE-ASSOCIATED KINASE REGULATOR 5-RELATED	coord: 1..271
IPR039619	Membrane-associated kinase regulator 2/5	PANTHER	PTHR33929	MEMBRANE-ASSOCIATED KINASE REGULATOR 2-RELATED	coord: 1..271

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg27016-RA	Carg27016-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0016301	kinase activity