Carg26971 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26971
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)H-hydrate epimerase
LocationCarg_Chr01: 11353632 .. 11358859 (+)
RNA-Seq ExpressionCarg26971
SyntenyCarg26971
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAGAACTTGGCCAATTATTTCAAGAAGCAACGGTGACAACTCGTCTTCTGGCGATGCGAAGCGGGTTGAGCCAGCGCAACTCCAATCAGGAATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGACACCGATAATTTCTCAATCTTCTCGCTCTCTTCGAATCCCTCTCTTCAATCTCCCACAGGTAAGTTCTTCGTACTGTTTTCTGTGTTTTGCCGCCTTTTTTCTAATCGGTTGTGTAGTAAATTTTGTTGTTAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTTAGCGTTGATCAGTTGATGGTTAGTTTTGTAATTGCTTGATTCATTGCTAGAAGTTCTTTTGCTGAGTTTCAATTTGTGAGTTCAAAATTGGTCTCATTCATCACTTTACAGTACTGAAAATTGAACTGTTGGTGTGTTTTTTTCTTCCCGTTTTTGTTTGTGTGTGTCCAGGAGCTGGCTGGATTGAGTGTAGCCACTTCAATAGCAGAGGTAAGCTTCCTCTTGGCGTTAATTTTTTTGTAAAGATCGTTCACACTCAATGGTTAGTGAAAGTGGATTGTGTTCTGGCAACTGTTTCGTTCTTGTTTATGAATGATTGTTATGAATCCTTATTTGGCTTATCTTGTTTGGTTAGTGAAAGTAGATATATGTAAGTTAACGTACAATGTTCATTGTTTGGCTAATCTTCTTTTGATTTGCAATTTTCATTGCTCTATATTTGAACCATTAATGAGCATCTTTCTTACGAGTTATAAGGTGGTTTGGGCCAATCTTTGTGTGAGATCTCACATCGGTTGGGGAGGAGAATGAAACATTTTTATAAGAGTGTGGAAACCTCACCCTAACAGACACGTTTTAGAAACCTTGAGGGGAACCCGAAAGGGAAAGCCTAAAGAGGACAATATCTGCTAGCGGTAGTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAATCACCCTTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGGATCTGGGTCGTTACAAATGGTATCAGAGCCAGTCACCGGACGATGTGCCGGCCTTCTCGCTCTTCCCCGAAGGGGGGTAGACACGAGGCGGTGTGCCAGTAAGGACACTGGACCTCAAAAGGGGGTGGATTTGGGGGCGGTCCCACATTGATTGGATAAAGGAACGAGTGCCAGCGGTGCCAGCGAGGACGCTGGGCCCCGAAGGGGGGTGGATTGTGATGTCCCACATTGGTTGGGGAGGAGAACAAATCACCCTTTATAAGGGTGTGGAAACCTTCCCCTAGCAGACGCGTTTTAAAGCCTTGAGGGGAAGCCCGAAAGGGAAAACCCAAAGAGGACAATATCTGCTAGCGGTGGATCTGGGTCGTTACAAGGCTTGGGCCATTACAAATGGTGTAAGAGCCAGACACGAGGCGATGTGTCAGCGAGAAGGCTGAACCCTGTAGGGAGTGGATACGAGACGGTGTGCCAGCAAGGACGCTGGCCCCGAAGAGGGTGGATTGGGGGTCTCACATCGATTGGAGAAGGGAACGAGTGCAAGAGAGGACCCTGGACCCCAAAGGGGGTGGATTGTAAGATCTCACGTTGGTTGGGGAGGAGAATGGAGCATTCTTTATAAGGATAAGAAAACCTTTCCCTAACAGACGCGTTTTAAAAACCTTGAGGGGAAGCCCGAAACGAAAAGTCCAAATAGGACAATATCTGCTAGCAGTGGGCTTGAGCCGTTACACTGTGTCTAGCTCAACCAAGTAGGATGAGGAGGTTAAATAGAATTTAAAAAGACATACTTTTGTTTAGCAAATAGCTGAGAACTTCTGTTTTCCTCCCAAAAAATCAACAGGTTTACAAAGTCAATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCGAAGCCTCTTTATGCTGGTTTGGTTACTCAGGTTGTGTTTCTCAACCATAATTGTAATCTTTTCCCTGTTCATCACTAGTTTGGCTGAAGTTCTTATCTAATTTCACGGTTTTGTAGCTGGAATCACTGTCAGTTCCTTTCGTGTCCGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTAATATCTTCCTGCTATACGCGCTCAATATATTATTTCAAAGAATTAAACTTTCGACCTTACCATCAATTAGTCATTTCTTCTTTAAGATGATCCTAGATTTGTTTCTAATTTGTGGGTTTTTGGGTCGGTTTGTGCATACCTCGACTACTTTCAGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTATGCTACTGTTCGAATTTTCTATGACCACCGTAGAGGTTTTTTCTTTAATATTTAATCACAAAAATTTGTTTAAAAACCGATTAGATCATTAAACACTTATTTATGCTAGGTTTCTCTGACTGCTCCTAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGTATAGGAAAGCCTCCACAAGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAGGCTGATCCTATTCATCAGGTGAATACTATATTCTTATCGGTGTTTGCTTTTATATTTCTGTCAGATCCTGTGTCGGTTGGTCGGAGAGGGGGAACGAAACATTCCTTATAAGGGTGTAGAAACCTCTCCCTAGTAGACGCGTTTTAAAATCGTGAGGCTGACAGCGATACGTAACGGGCCAAGGTAGACAATATCTGCTAGCGGTGGGCTTAGACTCTTAAAACTTCCTTATTTGAAAAAGAGTCGTTTGTTAACTTTATTGTTTCTAGTAGTGAACCAGTAATTTTCTTGCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCGTGAGCTTTGATTCTCTTCTTTTGGCATTCACTTAATTGAAGATCATTTTCTCCCTCGCTCAATTTGATAACCCTTTAATTTCTGGTTCACTTTGCTGCTTCAGATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTTAGTATGCTAGACTATTATCATGTTGCAGCAAACAATTTTTTCCCGTGCGCCTTAAAATAACTCCTCTCATATTGTTCGTTGTTATCTGCAGGTACACAAACTATGGAAGTCGGAAAGCACACGATTTAGCTGAAAATCCGCGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTGAAATAACAGCTTGCAGTTTTAGTTTGAGTTGAAAATGTCAGTAGGGAGGCCATTTTTTCTGCCCATAAATTCCTCGAGTAACTCGCCCTTCTGTTTCCGTTCCTTGTGGATTCTAATTCATGGGTTCAACATATAGAAAATTAGGCGGTGAGCTTAGGTTGTTACAAATGGTATTATAGAGCCCATCACCAGGCGGTATACCAGTGAGGATGTTGGCTCCCAAGAGGGGGTGGATTGTGAGATCCCACATCAATTGGAGAGGGGAACGAAGCACTCTTTGTAAGGGCGTGAAAACCTCTCCTTAGCCGACGCGTTTTAAAACCTAGAGGAGAAGCCTAAAAGGGAAAGTCCAAAGAGGACAATATCCGCTAGCAATGGGCTTGAGCGGTTATATGTCATTAGCTTTTAGCATTTCCCCCACTTCATAATGCTCATCCTCAATGCTTTCAAGGCAATGCAAATGAATGTGCTTACTATATTAGATATTGTTTAATTGTTACCTTCTAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGACGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGGTCTGTATCAGTGCCATTCAATATATTGTAGATAATCAAAATGCTGAGATATTGATAGATTCATGATGTCCCTTTCTCATTCAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGGTATGTGAATCAATATAAGCTCATATCTGATTCAAAATACAAATGATCTTTGATAATTCATTAAGGAACATGTTCTTCTGTTTGCAGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAAATTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCACGACAGGTTATTTTCACCTCTCTAAGAACAGGTGGCTTTATGTCATTGGCCGAGTAGTTGAGTTGCGGCCTTGCAAGCCTTCTGTTACTTAACGTTCGAGCGTTTGAGCGTTTGAGCCCTTCTCAATCTAACTATAACTCAACAGTTAAAATATATGAACGTTTGAGCTTTAGAAAATAATAAACAAAAGGAAATGAACTTTCTTCTCACTCGTTTCTTATGCTTGTTTTCTCGACAGGTTGCAATATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTAGCTCCGTGATTGCCTCTTCCGTTACGTTAGGCTTGGAATTTCATCGTTCATGTACCTTTTCTTTACTTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTGTTCGTGTACCTTTTCTTTCCTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTTTTGTGT

mRNA sequence

AAAAGAACTTGGCCAATTATTTCAAGAAGCAACGGTGACAACTCGTCTTCTGGCGATGCGAAGCGGGTTGAGCCAGCGCAACTCCAATCAGGAATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGACACCGATAATTTCTCAATCTTCTCGCTCTCTTCGAATCCCTCTCTTCAATCTCCCACAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTTAGCGTTGATCAGTTGATGGAGCTGGCTGGATTGAGTGTAGCCACTTCAATAGCAGAGGTTTACAAAGTCAATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCGAAGCCTCTTTATGCTGGTTTGGTTACTCAGCTGGAATCACTGTCAGTTCCTTTCGTGTCCGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTTTCTCTGACTGCTCCTAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGTATAGGAAAGCCTCCACAAGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAGGCTGATCCTATTCATCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTACACAAACTATGGAAGTCGGAAAGCACACGATTTAGCTGAAAATCCGCGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGACGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAAATTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCACGACAGGTTGCAATATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTAGCTCCGTGATTGCCTCTTCCGTTACGTTAGGCTTGGAATTTCATCGTTCATGTACCTTTTCTTTACTTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTGTTCGTGTACCTTTTCTTTCCTTTTCATGCTTGTTTCCCCTCCCCCTCCCCTTTTGTGT

Coding sequence (CDS)

AAAAGAACTTGGCCAATTATTTCAAGAAGCAACGGTGACAACTCGTCTTCTGGCGATGCGAAGCGGGTTGAGCCAGCGCAACTCCAATCAGGAATGCGTCCATTTATGCGCACAGCTCTTTCTATGTCACGCTTGATTTCTGCCCACTCATTCCATCTCAAATCGAACTCGACACCGATAATTTCTCAATCTTCTCGCTCTCTTCGAATCCCTCTCTTCAATCTCCCACAGGGTGTTTATCGGCCGAATTCGAGCTCTGAGTTTCGTTGGTTTTGCTCGAAGTCTCGGATTGCTGCTGCAACCATGCAGAATCGGGAGTCGATTTCGTATCTGACGCAGAAGGAAGCCGCTGAGGTCGATGAAATTCTCATGGGTCCTCTCGGGTTTAGCGTTGATCAGTTGATGGAGCTGGCTGGATTGAGTGTAGCCACTTCAATAGCAGAGGTTTACAAAGTCAATGAGTATAAGCGCGTTCTTGTTATCTGTGGCCCTGGAAACAATGGTGGAGATGGTCTCGTGGCTGCTCGTCATCTGTTTCACTTCGGATACAATCCATCAATTTGTTATCCAAAGAGGACTCCGAAGCCTCTTTATGCTGGTTTGGTTACTCAGCTGGAATCACTGTCAGTTCCTTTCGTGTCCGTAGAGGACTTGCCTTCGGATTTGTCGAAGGATTTTGACATTATAATCGATGCAATCTTTGGGTTCTCCTTCCACGGTGCTCCAAGGCCACCATTCAATGATTTGATCCAAAGGCTTGTCTCTTTAAATGAGTACAAGGAAGCAAAGCAAAGATTTCCTGTTGTTGTTTCTGTAGATATTCCATCCGGATGGCATGTCGAAGAAGGGGATATTAGTGATAGCAGCTTTAAACCCGATATGTTGGTTTCTCTGACTGCTCCTAAGCTATGTGCAAAGAGATTCCATGGTCCACATCATTTTTTAGGTGGCAGATTTGTGCCACCGTCTATCGTGAGCAAATTTAACCTTCATCTTCCTCCTTATCCCGGCACGGCAATGTGTGTTCGTATAGGAAAGCCTCCACAAGTTGATATAGCTGCACTTAGAGAAAACTATATTTCTCCTGAATTTGTTGAAGATAATGTAGAGGCTGATCCTATTCATCAGTTCCGCAAATGGTTCGATGAAGCAATTGCTGCTAATTTACGGGAACCTAATGCTATGTCGTTGTCTACTGCTACGAGCGATGGAAAACCATCATCTCGTATGGTTTTGCTAAAAGGATTTGATGAAGATGGTTTTGTCTGGTACACAAACTATGGAAGTCGGAAAGCACACGATTTAGCTGAAAATCCGCGTGCTTCGCTTCTTTTCTTCTGGGACGGTCTTAATAGGCAGGTAAGAGTGGAAGGAACAGTGCAGAAAGTTTCCGAAGACGAATCGGAGCAGTACTTCCACAGTCGTCCTCGTGGAAGTCAGCTCGGTGCAATCGTTAGCCCACAGAGTTCTGTGGTTCCTGGAAGACATTTTCTCATTGATAAGTTCAAAGAACTAGAGGATAGATACTCGAATGGAAGTTTGATTCCAAAACCAAAATACTGGGGCGGATACAGGCTTAAACCAGAAAATTTTGAGTTTTGGCAGGGGCAGCCATCTCGGCTGCACGACAGGTTGCAATATTCCCCTTGCGAGATAGAGGGAAAAAAGGTTTGGAAGGTAGAACGGTTAGCTCCGTGA

Protein sequence

KRTWPIISRSNGDNSSSGDAKRVEPAQLQSGMRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP
Homology
BLAST of Carg26971 vs. NCBI nr
Match: KAG7037612.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1161.7 bits (3004), Expect = 0.0e+00
Identity = 565/565 (100.00%), Postives = 565/565 (100.00%), Query Frame = 0

Query: 1   KRTWPIISRSNGDNSSSGDAKRVEPAQLQSGMRPFMRTALSMSRLISAHSFHLKSNSTPI 60
           KRTWPIISRSNGDNSSSGDAKRVEPAQLQSGMRPFMRTALSMSRLISAHSFHLKSNSTPI
Sbjct: 1   KRTWPIISRSNGDNSSSGDAKRVEPAQLQSGMRPFMRTALSMSRLISAHSFHLKSNSTPI 60

Query: 61  ISQSSRSLRIPLFNLPQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVD 120
           ISQSSRSLRIPLFNLPQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVD
Sbjct: 61  ISQSSRSLRIPLFNLPQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVD 120

Query: 121 EILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKRVLVICGPGNNGGDGLVAARHLFH 180
           EILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKRVLVICGPGNNGGDGLVAARHLFH
Sbjct: 121 EILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKRVLVICGPGNNGGDGLVAARHLFH 180

Query: 181 FGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHG 240
           FGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHG
Sbjct: 181 FGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHG 240

Query: 241 APRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTA 300
           APRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTA
Sbjct: 241 APRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTA 300

Query: 301 PKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPQVDIAALRENYI 360
           PKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPQVDIAALRENYI
Sbjct: 301 PKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPQVDIAALRENYI 360

Query: 361 SPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDG 420
           SPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDG
Sbjct: 361 SPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDG 420

Query: 421 FVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESEQYFHSRPRGSQ 480
           FVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESEQYFHSRPRGSQ
Sbjct: 421 FVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESEQYFHSRPRGSQ 480

Query: 481 LGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPENFEFWQGQPSR 540
           LGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPENFEFWQGQPSR
Sbjct: 481 LGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPENFEFWQGQPSR 540

Query: 541 LHDRLQYSPCEIEGKKVWKVERLAP 566
           LHDRLQYSPCEIEGKKVWKVERLAP
Sbjct: 541 LHDRLQYSPCEIEGKKVWKVERLAP 565

BLAST of Carg26971 vs. NCBI nr
Match: KAG6608269.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. NCBI nr
Match: XP_022941315.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurbita moschata])

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 529/534 (99.06%), Postives = 531/534 (99.44%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIA VYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAGVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDE GFVWYTNYGSRKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEYGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. NCBI nr
Match: XP_022980894.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurbita maxima])

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 524/534 (98.13%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRL+SAHSFHLKSN+TPIIS SSRSLRIPLF LPQGV RPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLVSAHSFHLKSNATPIISHSSRSLRIPLFKLPQGVCRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLP DLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPLDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. NCBI nr
Match: XP_023524154.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 525/534 (98.31%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRLISAHSFHLKSNS PIIS SSRSLRIPLF LPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSIPIISHSSRSLRIPLFKLPQGVYRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           V+EYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VDEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPP VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPHVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGS+KAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSQKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPEAFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. ExPASy Swiss-Prot
Match: Q9LTX3 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PPOX1 PE=1 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 3.9e-219
Identity = 367/535 (68.60%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPII-SQSSRSLRIPLFNLPQGVYRPNSSSEFRW 91
           MR  +R   +M+      +F L+S   PI  S    SL     +  Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 92  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 151
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 152 KVNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 211
           K  EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 212 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 271
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 272 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 331
           SVDIPSGWHVEEGD  D   KPDMLVSLTAPKLCAKRF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDMLVSLTAPKLCAKRFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 332 HLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 391
            LP YPGT+MCVRIGKPP+VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 392 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGL 451
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 452 NRQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 511
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 480

Query: 512 NGSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           +GS+IPKPK WGG+RLKP  FEFWQGQPSRLHDRLQYS  ++ G   WK+ RLAP
Sbjct: 481 DGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLHDRLQYSLQDVNGNPAWKIHRLAP 530

BLAST of Carg26971 vs. ExPASy Swiss-Prot
Match: C3YDS7 (NAD(P)H-hydrate epimerase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_104103 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.5e-66
Identity = 125/240 (52.08%), Postives = 162/240 (67.50%), Query Frame = 0

Query: 108 ISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKR---VLVICGP 167
           ISY++Q+EA ++D+ L     +SVDQLMELAG S A ++A+ Y +   K+   VLV CGP
Sbjct: 44  ISYVSQEEAQQIDQELFNEYAYSVDQLMELAGHSCAVALAKSYPLTSLKKDATVLVCCGP 103

Query: 168 GNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSDLSK 227
           GNNGGDGLV ARHL  FGYNPS+ YPKRT KPLY  L  Q E L +PF+S    P  LS 
Sbjct: 104 GNNGGDGLVCARHLKMFGYNPSVFYPKRTDKPLYKNLTIQCEQLDIPFLSHLPKPQLLSD 163

Query: 228 DFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVEEGD 287
            F  I+DA+FGFSF G  RPPF D+++ L         K+    + S+D+PSGW VE G 
Sbjct: 164 GFSYIVDALFGFSFKGEVRPPFGDVLKTL---------KEVTVPICSIDVPSGWDVEGG- 223

Query: 288 ISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMCVRI 345
            +    +P+ L+SLTAPK CA++F G +H+LGGRFVPP I+ K+ L+LP YPGT  C+R+
Sbjct: 224 -NPDGLQPEFLISLTAPKKCAEKFAGRYHYLGGRFVPPGIIQKYELNLPTYPGTEPCIRL 272

BLAST of Carg26971 vs. ExPASy Swiss-Prot
Match: Q7WG77 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella bronchiseptica (strain ATCC BAA-588 / NCTC 13252 / RB50) OX=257310 GN=pdxH PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.9e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 350 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 409
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 410 MVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESE 469
           +VL+KG+D+ GFV++TNY SRK  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 470 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 529
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 530 NFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
            FEFWQG+PSRLHDRL+Y P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Carg26971 vs. ExPASy Swiss-Prot
Match: Q7W4Q1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella parapertussis (strain 12822 / ATCC BAA-587 / NCTC 13253) OX=257311 GN=pdxH PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.9e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 350 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 409
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 410 MVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESE 469
           +VL+KG+D+ GFV++TNY SRK  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 470 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 529
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 530 NFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
            FEFWQG+PSRLHDRL+Y P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Carg26971 vs. ExPASy Swiss-Prot
Match: Q7VU72 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) OX=257313 GN=pdxH PE=3 SV=1)

HSP 1 Score: 251.1 bits (640), Expect = 2.9e-65
Identity = 118/216 (54.63%), Postives = 157/216 (72.69%), Query Frame = 0

Query: 350 VDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTATSDGKPSSR 409
           + ++ LR++Y     VE+   A P  QF +WFDEA+AA + EPNAM+L+T  ++G+PS+R
Sbjct: 1   MSVSDLRQSYEKGVLVEEQAAASPFQQFARWFDEAVAARVPEPNAMTLATVNAEGQPSAR 60

Query: 410 MVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQKVSEDESE 469
           +VL+KG+D+ GFV++TNY SRK  DL  NPRASLLFFW  L RQVR+EG ++KVS  ES+
Sbjct: 61  IVLIKGYDDAGFVFFTNYESRKGLDLDANPRASLLFFWQPLERQVRIEGVIEKVSAAESD 120

Query: 470 QYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPE 529
           +YFHSRP GS+LGA  S QS  +  R  L  + +E  DRY  G   P+P +WGGYRLKP 
Sbjct: 121 EYFHSRPLGSRLGAWASRQSQPIT-RDELEAREREFRDRY--GEHPPRPPHWGGYRLKPN 180

Query: 530 NFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
            FEFWQG+PSRLHDRL+Y P   +GK+ W ++RL+P
Sbjct: 181 RFEFWQGRPSRLHDRLRYEP---DGKQGWTIDRLSP 210

BLAST of Carg26971 vs. ExPASy TrEMBL
Match: A0A6J1FT85 (NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=1)

HSP 1 Score: 1090.1 bits (2818), Expect = 0.0e+00
Identity = 529/534 (99.06%), Postives = 531/534 (99.44%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIA VYK
Sbjct: 61  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAGVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDE GFVWYTNYGSRKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEYGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRL+YSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLRYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. ExPASy TrEMBL
Match: A0A6J1J0H2 (NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1)

HSP 1 Score: 1084.3 bits (2803), Expect = 0.0e+00
Identity = 524/534 (98.13%), Postives = 529/534 (99.06%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPFMRTALSMSRL+SAHSFHLKSN+TPIIS SSRSLRIPLF LPQGV RPNSSSEFRWF
Sbjct: 1   MRPFMRTALSMSRLVSAHSFHLKSNATPIISHSSRSLRIPLFKLPQGVCRPNSSSEFRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
           CSKSRIAAATMQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYK
Sbjct: 61  CSKSRIAAATMQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
           VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP
Sbjct: 121 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           FVSVEDLP DLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS
Sbjct: 181 FVSVEDLPLDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEGTVQKVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN
Sbjct: 421 RQVRVEGTVQKVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPKYWGGYRLKPE FEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP
Sbjct: 481 GSLIPKPKYWGGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 534

BLAST of Carg26971 vs. ExPASy TrEMBL
Match: A0A6J1E2J2 (NAD(P)H-hydrate epimerase OS=Momordica charantia OX=3673 GN=LOC111025403 PE=3 SV=1)

HSP 1 Score: 965.3 bits (2494), Expect = 1.1e-277
Identity = 469/550 (85.27%), Postives = 502/550 (91.27%), Query Frame = 0

Query: 16  SSGDAKRVEPAQLQSGMRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNL 75
           S+ DAK   PA+  + MR  +R   +M+R   AHSF LK NS+ IIS SSR L+IP   L
Sbjct: 23  SASDAK---PAKAPTEMRSLLRKTQAMTRSFLAHSFSLKPNSSSIISHSSRPLQIPFSKL 82

Query: 76  PQGVYRPNSSSEFRWFCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLM 135
            QGV RP SSS FRWFCSKSRI AA+MQN ESISYLTQ+EAAEVDEILMGPLGFSVDQLM
Sbjct: 83  SQGVSRPYSSSAFRWFCSKSRIEAASMQNWESISYLTQREAAEVDEILMGPLGFSVDQLM 142

Query: 136 ELAGLSVATSIAEVYKVNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPK 195
           ELAGLSVATSIAE YK +E KRVL ICGPGNNGGDGLVAARHLFHFGY PSICYPK+TPK
Sbjct: 143 ELAGLSVATSIAEAYKASECKRVLAICGPGNNGGDGLVAARHLFHFGYKPSICYPKQTPK 202

Query: 196 PLYAGLVTQLESLSVPFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVS 255
           PLYAGLVTQLESLSVPF+SVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVS
Sbjct: 203 PLYAGLVTQLESLSVPFISVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVS 262

Query: 256 LNEYKEAKQRFPVVVSVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFL 315
           LNEYKEA QRFPV+VSVDIPSGWHVEEGDIS+S+FKPDMLVSLTAPKL AK+FHG HHFL
Sbjct: 263 LNEYKEADQRFPVIVSVDIPSGWHVEEGDISNSNFKPDMLVSLTAPKLGAKKFHGSHHFL 322

Query: 316 GGRFVPPSIVSKFNLHLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIH 375
           GGRFVPPSIVSK+NLHLPPYPGTAMCVRIGKPPQVDIAALRENYISPEF+E+NVE +PIH
Sbjct: 323 GGRFVPPSIVSKYNLHLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFLEENVETNPIH 382

Query: 376 QFRKWFDEAIAANLREPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDL 435
           QFRKWFDEA+ ANLREPNAMSLSTATSDGKPSSRMVLLKG DEDGFVWYTNY SRKAHDL
Sbjct: 383 QFRKWFDEAVTANLREPNAMSLSTATSDGKPSSRMVLLKGVDEDGFVWYTNYESRKAHDL 442

Query: 436 AENPRASLLFFWDGLNRQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGR 495
           AEN RASLLF+W+GLNRQVRVEG+VQKVSE+ESEQYFHSRPRGSQLGAI SPQSSVV GR
Sbjct: 443 AENARASLLFYWEGLNRQVRVEGSVQKVSEEESEQYFHSRPRGSQLGAIASPQSSVVAGR 502

Query: 496 HFLIDKFKELEDRYSNGSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGK 555
           H LI+++KELEDR+SNGS IPKPK+WGGYRLKPE FEFWQGQ SRLHDRLQYSPCEI+GK
Sbjct: 503 HVLIERYKELEDRFSNGSFIPKPKHWGGYRLKPELFEFWQGQQSRLHDRLQYSPCEIDGK 562

Query: 556 KVWKVERLAP 566
           +VWKVERLAP
Sbjct: 563 RVWKVERLAP 569

BLAST of Carg26971 vs. ExPASy TrEMBL
Match: A0A6J1J0L7 (NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.1e-276
Identity = 459/464 (98.92%), Postives = 462/464 (99.57%), Query Frame = 0

Query: 102 MQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYKVNEYKRVLVI 161
           MQNRES+SYLTQKEAAEVDEILMGPLGFSV+QLMELAGLSVATSIAEVYKVNEYKRVLVI
Sbjct: 1   MQNRESVSYLTQKEAAEVDEILMGPLGFSVEQLMELAGLSVATSIAEVYKVNEYKRVLVI 60

Query: 162 CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPSD 221
           CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLP D
Sbjct: 61  CGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVPFVSVEDLPLD 120

Query: 222 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 281
           LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE
Sbjct: 121 LSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVSVDIPSGWHVE 180

Query: 282 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 341
           EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC
Sbjct: 181 EGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLHLPPYPGTAMC 240

Query: 342 VRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 401
           VRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT
Sbjct: 241 VRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLREPNAMSLSTAT 300

Query: 402 SDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 461
           SDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ
Sbjct: 301 SDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLNRQVRVEGTVQ 360

Query: 462 KVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 521
           KVSE+ESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW
Sbjct: 361 KVSEEESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSNGSLIPKPKYW 420

Query: 522 GGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GGYRLKPE FEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP
Sbjct: 421 GGYRLKPETFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 464

BLAST of Carg26971 vs. ExPASy TrEMBL
Match: A0A1S3CQL7 (NAD(P)H-hydrate epimerase OS=Cucumis melo OX=3656 GN=LOC103503675 PE=3 SV=1)

HSP 1 Score: 956.4 bits (2471), Expect = 5.2e-275
Identity = 459/534 (85.96%), Postives = 495/534 (92.70%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPIISQSSRSLRIPLFNLPQGVYRPNSSSEFRWF 91
           MRPF+R    M+RL   H  H  SNST II QSSRS +IPLFN+P GV+RPNSSS  RWF
Sbjct: 1   MRPFLRKTHLMTRLFFTH--HSFSNSTLIIPQSSRSFQIPLFNIPHGVFRPNSSSGIRWF 60

Query: 92  CSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVYK 151
            SKSR+ A  MQN E+ISYL+Q+EAAEVDEILMG LGFSVDQLMELAGLSVATS+AEVYK
Sbjct: 61  SSKSRVEA--MQNLEAISYLSQREAAEVDEILMGSLGFSVDQLMELAGLSVATSVAEVYK 120

Query: 152 VNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSVP 211
            +EYKRVLVICGPGNNGGDGLVAARHLFHFGY PSICYPKRT KPLY GLVTQLESLSVP
Sbjct: 121 ASEYKRVLVICGPGNNGGDGLVAARHLFHFGYKPSICYPKRTAKPLYTGLVTQLESLSVP 180

Query: 212 FVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVVS 271
           F++V+DLP DLSKDFDIIIDAIFGFSFHGAPRPPF+DLIQRL SLN+YKEA QR P +VS
Sbjct: 181 FLTVDDLPLDLSKDFDIIIDAIFGFSFHGAPRPPFDDLIQRLTSLNDYKEANQRSPAIVS 240

Query: 272 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNLH 331
           VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAK+F+GPHHFLGGRFVPPSIV+K+NLH
Sbjct: 241 VDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKKFNGPHHFLGGRFVPPSIVNKYNLH 300

Query: 332 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLRE 391
           LPPYPGTAMCVRIGKPPQVDIAALRENYISPEF+E+NV+ADPI QFRKWFD+A+AANLRE
Sbjct: 301 LPPYPGTAMCVRIGKPPQVDIAALRENYISPEFLEENVDADPIRQFRKWFDDAVAANLRE 360

Query: 392 PNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGLN 451
           PNAMSLSTATSDGKPSSRMVLLKG DEDGFVWYTNY SRKAHDLAENPRASLLFFW+GLN
Sbjct: 361 PNAMSLSTATSDGKPSSRMVLLKGVDEDGFVWYTNYESRKAHDLAENPRASLLFFWEGLN 420

Query: 452 RQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYSN 511
           RQVRVEG+VQKVSE+ESEQYFHSRP+GSQLGAI SPQSSVVPGRHFLI+K+KELED +SN
Sbjct: 421 RQVRVEGSVQKVSEEESEQYFHSRPQGSQLGAIASPQSSVVPGRHFLINKYKELEDTFSN 480

Query: 512 GSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           GSLIPKPK+WGGYRLKPE FEFWQGQPSRLHDRLQYSPC+I+GKK WKVERLAP
Sbjct: 481 GSLIPKPKHWGGYRLKPELFEFWQGQPSRLHDRLQYSPCDIDGKKAWKVERLAP 530

BLAST of Carg26971 vs. TAIR 10
Match: AT5G49970.1 (pyridoxin (pyrodoxamine) 5'-phosphate oxidase )

HSP 1 Score: 762.3 bits (1967), Expect = 2.7e-220
Identity = 367/535 (68.60%), Postives = 422/535 (78.88%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPII-SQSSRSLRIPLFNLPQGVYRPNSSSEFRW 91
           MR  +R   +M+      +F L+S   PI  S    SL     +  Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 92  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 151
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 152 KVNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 211
           K  EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 212 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 271
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 272 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 331
           SVDIPSGWHVEEGD  D   KPDMLVSLTAPKLCAKRF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDMLVSLTAPKLCAKRFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 332 HLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 391
            LP YPGT+MCVRIGKPP+VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 392 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGL 451
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 452 NRQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 511
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 480

Query: 512 NGSLIPKPKYWGGYRLKPENFEFWQGQPSRLHDRLQYSPCEIEGKKVWKVERLAP 566
           +GS+IPKPK WGG+RLKP  FEFWQGQPSRLHDRLQYS  ++ G   WK+ RLAP
Sbjct: 481 DGSVIPKPKNWGGFRLKPNLFEFWQGQPSRLHDRLQYSLQDVNGNPAWKIHRLAP 530

BLAST of Carg26971 vs. TAIR 10
Match: AT5G49970.2 (pyridoxin (pyrodoxamine) 5'-phosphate oxidase )

HSP 1 Score: 644.0 bits (1660), Expect = 1.1e-184
Identity = 318/482 (65.98%), Postives = 368/482 (76.35%), Query Frame = 0

Query: 32  MRPFMRTALSMSRLISAHSFHLKSNSTPII-SQSSRSLRIPLFNLPQGVYRPNSSSEFRW 91
           MR  +R   +M+      +F L+S   PI  S    SL     +  Q    P+  S  R 
Sbjct: 1   MRNVIRRVTTMT-----FTFLLQSPPLPISPSPPQFSLSSSPLSKTQRFITPSQGSRLRT 60

Query: 92  FCSKSRIAAATMQNRESISYLTQKEAAEVDEILMGPLGFSVDQLMELAGLSVATSIAEVY 151
            C+K  I          +SYLTQ+EAAE+DE LMGPLGFS+DQLMELAGLSVA SIAEVY
Sbjct: 61  LCTKVIIPNMQDSGSPPLSYLTQREAAEIDETLMGPLGFSIDQLMELAGLSVAASIAEVY 120

Query: 152 KVNEYKRVLVICGPGNNGGDGLVAARHLFHFGYNPSICYPKRTPKPLYAGLVTQLESLSV 211
           K  EY RVL ICGPGNNGGDGLVAARHL HFGY P ICYPKRT KPLY GLVTQL+SLSV
Sbjct: 121 KPEEYSRVLAICGPGNNGGDGLVAARHLHHFGYKPFICYPKRTAKPLYTGLVTQLDSLSV 180

Query: 212 PFVSVEDLPSDLSKDFDIIIDAIFGFSFHGAPRPPFNDLIQRLVSLNEYKEAKQRFPVVV 271
           PFVSVEDLP DLSKDFD+I+DA+FGFSFHGAPRPPF+DLI+RLVSL  Y++  Q+ PV+V
Sbjct: 181 PFVSVEDLPDDLSKDFDVIVDAMFGFSFHGAPRPPFDDLIRRLVSLQNYEQTLQKHPVIV 240

Query: 272 SVDIPSGWHVEEGDISDSSFKPDMLVSLTAPKLCAKRFHGPHHFLGGRFVPPSIVSKFNL 331
           SVDIPSGWHVEEGD  D   KPDML           RF GPHHFLGGRFVPPS+  K+ L
Sbjct: 241 SVDIPSGWHVEEGDHEDGGIKPDML-----------RFRGPHHFLGGRFVPPSVAEKYKL 300

Query: 332 HLPPYPGTAMCVRIGKPPQVDIAALRENYISPEFVEDNVEADPIHQFRKWFDEAIAANLR 391
            LP YPGT+MCVRIGKPP+VDI+A+R NY+SPE +E+ VE DP  QFRKWFDEA+AA LR
Sbjct: 301 ELPSYPGTSMCVRIGKPPKVDISAMRVNYVSPELLEEQVETDPTVQFRKWFDEAVAAGLR 360

Query: 392 EPNAMSLSTATSDGKPSSRMVLLKGFDEDGFVWYTNYGSRKAHDLAENPRASLLFFWDGL 451
           E NAM+LSTA  D KPSSRMVLLKGFDE+GFVW+TNY S+K  DL+ENP A+LLF+W+ L
Sbjct: 361 ETNAMALSTANKDKKPSSRMVLLKGFDENGFVWFTNYESKKGSDLSENPSAALLFYWEIL 420

Query: 452 NRQVRVEGTVQKVSEDESEQYFHSRPRGSQLGAIVSPQSSVVPGRHFLIDKFKELEDRYS 511
           NRQVR+EG V+++ E ESE YFHSRPRGSQ+GAIVS QSSVVPGRH L D+++EL  +YS
Sbjct: 421 NRQVRIEGPVERIPESESENYFHSRPRGSQIGAIVSKQSSVVPGRHVLYDEYEELTKQYS 466

Query: 512 NG 513
           +G
Sbjct: 481 DG 466

BLAST of Carg26971 vs. TAIR 10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein )

HSP 1 Score: 44.7 bits (104), Expect = 2.9e-04
Identity = 26/91 (28.57%), Postives = 47/91 (51.65%), Query Frame = 0

Query: 384 AIAAN--LREPNAMSLSTATSDGKPSSRMVLLKGFDE--DGFVWYTNYGSRKAHDLAENP 443
           AI AN  L   + + L+T   +G+PS+R V+ +GF+E  D     T+  SRK  +L   P
Sbjct: 15  AIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINTDLRSRKIEELKHCP 74

Query: 444 RASLLFFWDGLNRQVRVEGTVQKVSEDESEQ 471
            + + +++     Q R+ G ++ +     +Q
Sbjct: 75  FSEMCWYFSDTWEQFRINGRIEVIDASNPDQ 105

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037612.10.0e+00100.00Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbit... [more]
KAG6608269.10.0e+00100.00Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic, partial [Cucurbit... [more]
XP_022941315.10.0e+0099.06pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurb... [more]
XP_022980894.10.0e+0098.13pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X2 [Cucurb... [more]
XP_023524154.10.0e+0098.31pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic isoform X1 [Cucurb... [more]
Match NameE-valueIdentityDescription
Q9LTX33.9e-21968.60Pyridoxine/pyridoxamine 5'-phosphate oxidase 1, chloroplastic OS=Arabidopsis tha... [more]
C3YDS71.5e-6652.08NAD(P)H-hydrate epimerase OS=Branchiostoma floridae OX=7739 GN=BRAFLDRAFT_104103... [more]
Q7WG772.9e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella bronchiseptica (strai... [more]
Q7W4Q12.9e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella parapertussis (strain... [more]
Q7VU722.9e-6554.63Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Bordetella pertussis (strain Toh... [more]
Match NameE-valueIdentityDescription
A0A6J1FT850.0e+0099.06NAD(P)H-hydrate epimerase OS=Cucurbita moschata OX=3662 GN=LOC111446652 PE=3 SV=... [more]
A0A6J1J0H20.0e+0098.13NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1[more]
A0A6J1E2J21.1e-27785.27NAD(P)H-hydrate epimerase OS=Momordica charantia OX=3673 GN=LOC111025403 PE=3 SV... [more]
A0A6J1J0L76.1e-27698.92NAD(P)H-hydrate epimerase OS=Cucurbita maxima OX=3661 GN=LOC111480209 PE=3 SV=1[more]
A0A1S3CQL75.2e-27585.96NAD(P)H-hydrate epimerase OS=Cucumis melo OX=3656 GN=LOC103503675 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G49970.12.7e-22068.60pyridoxin (pyrodoxamine) 5'-phosphate oxidase [more]
AT5G49970.21.1e-18465.98pyridoxin (pyrodoxamine) 5'-phosphate oxidase [more]
AT2G46580.12.9e-0428.57Pyridoxamine 5'-phosphate oxidase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004443YjeF N-terminal domainPFAMPF03853YjeF_Ncoord: 132..304
e-value: 2.1E-35
score: 122.1
IPR004443YjeF N-terminal domainTIGRFAMTIGR00197TIGR00197coord: 113..308
e-value: 1.5E-45
score: 153.4
IPR004443YjeF N-terminal domainHAMAPMF_01966NADHX_epimerasecoord: 112..347
score: 20.584599
IPR004443YjeF N-terminal domainPROSITEPS51385YJEF_Ncoord: 116..352
score: 57.169556
IPR000659Pyridoxamine 5'-phosphate oxidaseTIGRFAMTIGR00558TIGR00558coord: 348..565
e-value: 1.7E-87
score: 290.5
IPR000659Pyridoxamine 5'-phosphate oxidaseHAMAPMF_01629PdxHcoord: 358..565
score: 39.077682
IPR036652YjeF N-terminal domain superfamilyGENE3D3.40.50.10260coord: 107..349
e-value: 3.2E-74
score: 251.2
IPR036652YjeF N-terminal domain superfamilySUPERFAMILY64153YjeF N-terminal domain-likecoord: 109..339
IPR011576Pyridoxamine 5'-phosphate oxidase, putativePFAMPF01243Putative_PNPOxcoord: 381..467
e-value: 3.7E-30
score: 103.9
IPR019576Pyridoxine 5'-phosphate oxidase, dimerisation, C-terminalPFAMPF10590PNP_phzG_Ccoord: 521..565
e-value: 4.8E-19
score: 68.1
IPR012349FMN-binding split barrelGENE3D2.30.110.10coord: 359..555
e-value: 9.0E-84
score: 281.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..24
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..23
NoneNo IPR availablePANTHERPTHR13232:SF13NAD(P)H-HYDRATE EPIMERASEcoord: 102..549
NoneNo IPR availableSUPERFAMILY50475FMN-binding split barrelcoord: 366..565
IPR032976YjeF N-terminal domain-containing protein NAXE-likePANTHERPTHR13232NAD(P)H-HYDRATE EPIMERASEcoord: 102..549
IPR019740Pyridoxamine 5'-phosphate oxidase, conserved sitePROSITEPS01064PYRIDOX_OXIDASEcoord: 531..544

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26971-RACarg26971-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042823 pyridoxal phosphate biosynthetic process
biological_process GO:0008615 pyridoxine biosynthetic process
molecular_function GO:0010181 FMN binding
molecular_function GO:0046872 metal ion binding
molecular_function GO:0052856 NADHX epimerase activity
molecular_function GO:0052857 NADPHX epimerase activity
molecular_function GO:0004733 pyridoxamine-phosphate oxidase activity
molecular_function GO:0016638 oxidoreductase activity, acting on the CH-NH2 group of donors