Carg26888 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26888
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionphospholipase A(1) LCAT3
LocationCarg_Chr05: 5425547 .. 5428560 (-)
RNA-Seq ExpressionCarg26888
SyntenyCarg26888
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAAGCCAATTTTTCGTATCTAGATGGACATTTCACCAGCTGGTAACTTTCCTCCTGTCAGTGTCTAGGTGGTTTAGTTTACACCTAAAATGATTGCAAGGGTATTTTTTTTAATCATTATCGACAAGTTGGATACCTTATCAACCATTTTTTCATGCATATCTTTGTCTCATTAATGATATTGTGCTTTTGTTCTCAATGATACCATTTCTTTGTGTATGATATGTCATAGGTCTACTAATGATATTTACGTGCAGTTGGTTGAATGTCCTTCAATTTATGAGATGCTGGGGAATCTAGGATTCAATTGGCATGCACAACCACAGATCCAAGTTTGGCAAAAGAGTTCTGTTGATGGAAAAACTTCTGTTGAATTGAAATCATATGGCCCAACTGATAGCATTGCTTTATTCGAAGAAGCATTAAGAAATAACCAGGTACGACACTTCACGTTGATTCTGTTAAAAAGTCATCTGGCACAAGCTAATTTTAATTTCATTATTGCAGATAAAATTTCATGGAAAGACTATTCCTCTGCCCTTTAACTTGGATATTCTCAAATGGGCTACTGGTACTCGCCAAGTAATTGATAATGCAAGGCTACCAGATGGAATCTCCTTCTATAATATTTATGGGACATCGTTCGATACGCCTTTTGATGTGTGGTATGTTAATGAACTTTGCATTATTATGTGTTAGATGCATATTTAGTTTTCAGTAAAAAGACTGTCTTTCATATCATTGTAATGATAAATTATGAATGGGTCAGTGTAGTAGTCCAGAGGTATGGCTGTTTTCTCATTGCTTTGTATAAATTTGATATCAATTCATTGATGTTAATGCCTCTGTTTCAAATTAAATAATGAACATATGGTATTAACTATGATGCAGCTATGGTTCCGAGTCGTTGCCGATTGAGGACTTGTCTGAAATCTGCAAAACTTTGGTGAGTGATTCAATTTCCTTTTGCTTTTATGTGTTAGGCCTTTCTTCTTCTACTTAAGATAGACATTGTTCGGGATTTCAACCTTTTTTTTTTTTCCTGATTGGATTGTTTGTTGGGTTCACTGTTCATGGTTTTAAGATGGTCTATTTGTTTGGACAATCGCATCATCTTTTAGTCTCTTGTTTGAATATGCTGTGTGAGATCCCAGATCGATTGGGGAGGAGAACGAAACACCCTTATAAAGGTGTGGAAACCTCTCCCTAGTAGACGCGTTTTAAAAACTTTGATGGGAAGTCCGAAAGGAAAAGTCCAAAGAGGACAATATCTCCTAGCGGTGGGCCTGGGCCGTTCCATACACAATGTTAAAAGACAAGTCATTGATGGCTAAAGTTCCTGACACATGCTGAACAACCTTACTGTCTAAATTACTACTATTCAACATAATGGGGATGTCTTTCTTCATTGGTCCCGAACTGTTTGGATCGATTTCTTAAGTATCATACCAAATCCTATCAATAGAATAGCTTTCTCGAGAAATACGAGACATAAATCCTTTTTAGCCCAAATTTTTGTCAGTGGCGATCTCCAATTACAAAGAGACTTTCGACACCCAAACATCATCTGTCCTGAGCTTACTAGCCCTTCAACACCAAACATGGAAAATTCTTCTTAAAGAGGAGATATTACTAAATGACCTTTATCTTTATTTATTTTTTGTTTTTAATTTCCCGTCTTTTCTGGCTTTGATTAATTTATCAACAGTTTGGATGTTGAAGTAAATACTGTTTTTGATGCATTCCCATTTTCACTGATCAACCGAATAACAATTTTTTAAATGTGATAACAATGAGGTGAATGGGGACCTCTGAGTAATATGATATTCTTTATTGTGTATAAGAACTAACAATATATGACTGACTGCTTATCACCCTTGTTTCTCTTCCTCTCGGCAGCCTCAGTATTCTTATGTGAACGGAGACGGTACAGTTCCCAGTGAATCAGCCAAGGTATGCTATTTCACCAAATTTAGTTCATGCATTCAAAAAACATGTTTGTTGTCGTTGTGTTCGTGTGCGCGTGTGTTTTATTTACATAACGTGCATCTTTCCTTTGGAAGGCGGATGGTTTTGAAGCAACTGAGAGAGTAGGAGTCGCAGCGAGTCATCGAGGACTTTTGAAGGACGAAACAGTATTGCAGCTTATCCAGAAATGGTTGGGAATTGATGAGAAGGTTAGTAAACACCCAACAACTTCCAAAGTGTTCGATGCTTCTCTGAAGTAGAAATGTGGCTAGTGTAAGTTTAAAAATAAGGCATAAATATTGTATTGCTGAAGCAAATCCAAGTACAGTCATTTGTATTCTAGTATATCAGTAGAATCATAATGACGAGATTGCCCAAATTAAGGATAGAAACACATACATACATGACTCGCACACATACATTATCAAAATCATGGCTTTACAAACCTAAAAAAAATCATTCACATGACTAGCGTGCTTTACGTTTTAAAATGACTTGGGACTTTGTTTTCAGTAACTTATCATACCTATAAGTTGTAGATTGAAGCTTGCAATTGGCTTCCAACTTCGGGTAACTTTGAATTTCAATTGTCTTATTTGCAACTATACACGAGAATATCGTAAGTTAGGAGCTATACTACAACGCCTACCTTGAGTCCAAAAAGGCTTATTAGAGTATTACCTAATGAGTGAGTAGAGTTTGAGATCTAAATCACCTAGACGTCCTAAGAATTTAGTAAGCCTCTACGGTATCCAAAGAAATTAAAGAAAGCTTACGAGATTGCTAACAAGGTCAACGGTATCACCAGAGTATGACAGCCTTCAATTTTCTCTAGTACCTTAATAGCACTATTTGATGACCTAAGTTGAACCAATAGTGTCCCTGCCAAGTGTTAGTATCTAGAAACCAGTATTGTCAAACTTGATTAAGGTTAAATAAGATGATATGTAGTGACGACATGGAGTTACTATTGTTGTGTAGAGATGGATCGATAGCCTAGGTTGTGATACCTTAAACTCGTCTTGAGAGTGATCAAAGGGACTG

mRNA sequence

ATGAAAGCCAATTTTTCGTATCTAGATGGACATTTCACCAGCTGGTCTACTAATGATATTTACGTGCAGTTGGTTGAATGTCCTTCAATTTATGAGATGCTGGGGAATCTAGGATTCAATTGGCATGCACAACCACAGATCCAAGTTTGGCAAAAGAGTTCTGTTGATGGAAAAACTTCTGTTGAATTGAAATCATATGGCCCAACTGATAGCATTGCTTTATTCGAAGAAGCATTAAGAAATAACCAGATAAAATTTCATGGAAAGACTATTCCTCTGCCCTTTAACTTGGATATTCTCAAATGGGCTACTGGTACTCGCCAAGTAATTGATAATGCAAGGCTACCAGATGGAATCTCCTTCTATAATATTTATGGGACATCGTTCGATACGCCTTTTGATGTGTGCTATGGTTCCGAGTCGTTGCCGATTGAGGACTTGTCTGAAATCTGCAAAACTTTGCCTCAGTATTCTTATGTGAACGGAGACGGTACAGTTCCCAGTGAATCAGCCAAGGCGGATGGTTTTGAAGCAACTGAGAGAGTAGGAGTCGCAGCGAGTCATCGAGGACTTTTGAAGGACGAAACAGTATTGCAGCTTATCCAGAAATGGTTGGGAATTGATGAGAAGGTTAGTAAACACCCAACAACTTCCAAAGTGTTCGATGCTTCTCTGAAGTAGAAATGTGGCTAGTGTAAGTTTAAAAATAAGGCATAAATATTGTATTGCTGAAGCAAATCCAAGTACAGTCATTTGTATTCTAGTATATCAGTAGAATCATAATGACGAGATTGCCCAAATTAAGGATAGAAACACATACATACATGACTCGCACACATACATTATCAAAATCATGGCTTTACAAACCTAAAAAAAATCATTCACATGACTAGCGTGCTTTACGTTTTAAAATGACTTGGGACTTTGTTTTCAGTAACTTATCATACCTATAAGTTGTAGATTGAAGCTTGCAATTGGCTTCCAACTTCGGGTAACTTTGAATTTCAATTGTCTTATTTGCAACTATACACGAGAATATCGTAAGTTAGGAGCTATACTACAACGCCTACCTTGAGTCCAAAAAGGCTTATTAGAGTATTACCTAATGAGTGAGTAGAGTTTGAGATCTAAATCACCTAGACGTCCTAAGAATTTAGTAAGCCTCTACGGTATCCAAAGAAATTAAAGAAAGCTTACGAGATTGCTAACAAGGTCAACGGTATCACCAGAGTATGACAGCCTTCAATTTTCTCTAGTACCTTAATAGCACTATTTGATGACCTAAGTTGAACCAATAGTGTCCCTGCCAAGTGTTAGTATCTAGAAACCAGTATTGTCAAACTTGATTAAGGTTAAATAAGATGATATGTAGTGACGACATGGAGTTACTATTGTTGTGTAGAGATGGATCGATAGCCTAGGTTGTGATACCTTAAACTCGTCTTGAGAGTGATCAAAGGGACTG

Coding sequence (CDS)

ATGAAAGCCAATTTTTCGTATCTAGATGGACATTTCACCAGCTGGTCTACTAATGATATTTACGTGCAGTTGGTTGAATGTCCTTCAATTTATGAGATGCTGGGGAATCTAGGATTCAATTGGCATGCACAACCACAGATCCAAGTTTGGCAAAAGAGTTCTGTTGATGGAAAAACTTCTGTTGAATTGAAATCATATGGCCCAACTGATAGCATTGCTTTATTCGAAGAAGCATTAAGAAATAACCAGATAAAATTTCATGGAAAGACTATTCCTCTGCCCTTTAACTTGGATATTCTCAAATGGGCTACTGGTACTCGCCAAGTAATTGATAATGCAAGGCTACCAGATGGAATCTCCTTCTATAATATTTATGGGACATCGTTCGATACGCCTTTTGATGTGTGCTATGGTTCCGAGTCGTTGCCGATTGAGGACTTGTCTGAAATCTGCAAAACTTTGCCTCAGTATTCTTATGTGAACGGAGACGGTACAGTTCCCAGTGAATCAGCCAAGGCGGATGGTTTTGAAGCAACTGAGAGAGTAGGAGTCGCAGCGAGTCATCGAGGACTTTTGAAGGACGAAACAGTATTGCAGCTTATCCAGAAATGGTTGGGAATTGATGAGAAGGTTAGTAAACACCCAACAACTTCCAAAGTGTTCGATGCTTCTCTGAAGTAG

Protein sequence

MKANFSYLDGHFTSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQKWLGIDEKVSKHPTTSKVFDASLK
Homology
BLAST of Carg26888 vs. NCBI nr
Match: KAG7029921.1 (Phospholipase A(1) LCAT3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0

Query: 1   MKANFSYLDGHFTSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTS 60
           MKANFSYLDGHFTSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTS
Sbjct: 1   MKANFSYLDGHFTSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTS 60

Query: 61  VELKSYGPTDSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGIS 120
           VELKSYGPTDSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGIS
Sbjct: 61  VELKSYGPTDSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGIS 120

Query: 121 FYNIYGTSFDTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATE 180
           FYNIYGTSFDTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATE
Sbjct: 121 FYNIYGTSFDTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATE 180

Query: 181 RVGVAASHRGLLKDETVLQLIQKWLGIDEKVSKHPTTSKVFDASLK 227
           RVGVAASHRGLLKDETVLQLIQKWLGIDEKVSKHPTTSKVFDASLK
Sbjct: 181 RVGVAASHRGLLKDETVLQLIQKWLGIDEKVSKHPTTSKVFDASLK 226

BLAST of Carg26888 vs. NCBI nr
Match: KAG6598962.1 (Phospholipase A(1) LCAT3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 422.9 bits (1086), Expect = 1.7e-114
Identity = 203/203 (100.00%), Postives = 203/203 (100.00%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ
Sbjct: 253 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 312

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 313 IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 372

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK
Sbjct: 373 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 432

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGIDEKVSKHPTTSKVFDASLK
Sbjct: 433 WLGIDEKVSKHPTTSKVFDASLK 455

BLAST of Carg26888 vs. NCBI nr
Match: XP_022933151.1 (phospholipase A(1) LCAT3 [Cucurbita moschata])

HSP 1 Score: 416.4 bits (1069), Expect = 1.6e-112
Identity = 200/203 (98.52%), Postives = 201/203 (99.01%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ
Sbjct: 254 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 313

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFNLD+LKWATGTRQVIDNA LPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 314 IKFHGKTIPLPFNLDVLKWATGTRQVIDNASLPDGISFYNIYGTSFDTPFDVCYGSESLP 373

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK
Sbjct: 374 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 433

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGIDEKVSKH TTSKVFDASLK
Sbjct: 434 WLGIDEKVSKHLTTSKVFDASLK 456

BLAST of Carg26888 vs. NCBI nr
Match: XP_023546771.1 (phospholipase A(1) LCAT3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 412.5 bits (1059), Expect = 2.3e-111
Identity = 198/203 (97.54%), Postives = 200/203 (98.52%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ
Sbjct: 253 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 312

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFNLDILKWA GTRQVIDNARLPDGISFYNIYGTS DTPFDVCYGSESLP
Sbjct: 313 IKFHGKTIPLPFNLDILKWANGTRQVIDNARLPDGISFYNIYGTSLDTPFDVCYGSESLP 372

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK
Sbjct: 373 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 432

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGID+KVSKH TTSKVFDA+LK
Sbjct: 433 WLGIDQKVSKHLTTSKVFDAALK 455

BLAST of Carg26888 vs. NCBI nr
Match: XP_022973913.1 (phospholipase A(1) LCAT3 [Cucurbita maxima])

HSP 1 Score: 409.5 bits (1051), Expect = 1.9e-110
Identity = 195/203 (96.06%), Postives = 200/203 (98.52%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSV+L+SYGPTDSIALFEEALRNNQ
Sbjct: 251 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVQLRSYGPTDSIALFEEALRNNQ 310

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFN DIL+WATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 311 IKFHGKTIPLPFNFDILEWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 370

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKT PQYSYVNGDGTVPSESAKADGFEATERVGVAA+HRGLLKDETVLQLIQK
Sbjct: 371 IEDLSEICKTSPQYSYVNGDGTVPSESAKADGFEATERVGVAATHRGLLKDETVLQLIQK 430

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGIDEKVSKH TTSKVFDAS+K
Sbjct: 431 WLGIDEKVSKHLTTSKVFDASMK 453

BLAST of Carg26888 vs. ExPASy Swiss-Prot
Match: Q93V61 (Phospholipase A(1) LCAT3 OS=Arabidopsis thaliana OX=3702 GN=LCAT3 PE=1 SV=1)

HSP 1 Score: 258.5 bits (659), Expect = 7.3e-68
Identity = 120/203 (59.11%), Postives = 157/203 (77.34%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSV-DGKTSVELKSYGPTDSIALFEEALRNN 83
           LVECPSIYEM+ N  F W  QP+I+VW+K S  D  TSVEL+S+G  +SI LF +AL+NN
Sbjct: 244 LVECPSIYEMMANPDFKWKKQPEIRVWRKKSENDVDTSVELESFGLIESIDLFNDALKNN 303

Query: 84  QIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESL 143
           ++ + G  I LPFN  IL WA  TR++++ A+LPDG+SFYNIYG S +TPFDVCYG+E+ 
Sbjct: 304 ELSYGGNKIALPFNFAILDWAAKTREILNKAQLPDGVSFYNIYGVSLNTPFDVCYGTETS 363

Query: 144 PIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQ 203
           PI+DLSEIC+T+P+Y+YV+GDGTVP+ESA A  F+A   VGV+ SHRGLL+DE V +LIQ
Sbjct: 364 PIDDLSEICQTMPEYTYVDGDGTVPAESAAAAQFKAVASVGVSGSHRGLLRDERVFELIQ 423

Query: 204 KWLGIDEKVS--KHPTTSKVFDA 224
           +WLG++ K +  KH  T KV D+
Sbjct: 424 QWLGVEPKKAKRKHLRTHKVVDS 446

BLAST of Carg26888 vs. ExPASy Swiss-Prot
Match: Q71N54 (Lecithin-cholesterol acyltransferase-like 4 OS=Arabidopsis thaliana OX=3702 GN=LCAT4 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.1e-47
Identity = 90/200 (45.00%), Postives = 132/200 (66.00%), Query Frame = 0

Query: 13  TSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVW-QKSSVD--GKTSVELKSYGPT 72
           + WS + +   L+ECPSIYE++    F W   P +++W +K S D  G + V L+SY   
Sbjct: 241 SKWSMHQL---LIECPSIYELMCCPYFKWELPPVLELWREKESNDGVGTSYVVLESYCSL 300

Query: 73  DSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSF 132
           +S+ +F ++L NN   + G++I LPFN  I++WA  T+QV+ +A+LP  + FYNIYGT+ 
Sbjct: 301 ESLEVFTKSLSNNTADYCGESIDLPFNWKIMEWAHKTKQVLASAKLPPKVKFYNIYGTNL 360

Query: 133 DTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHR 192
           +TP  VCYG+E +P++DL+ +    P Y  V+GDGTVP ESA ADG EA  RVGV   HR
Sbjct: 361 ETPHSVCYGNEKMPVKDLTNLRYFQPTYICVDGDGTVPMESAMADGLEAVARVGVPGEHR 420

Query: 193 GLLKDETVLQLIQKWLGIDE 210
           G+L D  V ++++KWL + E
Sbjct: 421 GILNDHRVFRMLKKWLNVGE 437

BLAST of Carg26888 vs. ExPASy TrEMBL
Match: A0A6J1EYB8 (phospholipase A(1) LCAT3 OS=Cucurbita moschata OX=3662 GN=LOC111439951 PE=4 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 7.7e-113
Identity = 200/203 (98.52%), Postives = 201/203 (99.01%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ
Sbjct: 254 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 313

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFNLD+LKWATGTRQVIDNA LPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 314 IKFHGKTIPLPFNLDVLKWATGTRQVIDNASLPDGISFYNIYGTSFDTPFDVCYGSESLP 373

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK
Sbjct: 374 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 433

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGIDEKVSKH TTSKVFDASLK
Sbjct: 434 WLGIDEKVSKHLTTSKVFDASLK 456

BLAST of Carg26888 vs. ExPASy TrEMBL
Match: A0A6J1IEK6 (phospholipase A(1) LCAT3 OS=Cucurbita maxima OX=3661 GN=LOC111472543 PE=4 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 9.4e-111
Identity = 195/203 (96.06%), Postives = 200/203 (98.52%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSV+L+SYGPTDSIALFEEALRNNQ
Sbjct: 251 LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVQLRSYGPTDSIALFEEALRNNQ 310

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFN DIL+WATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 311 IKFHGKTIPLPFNFDILEWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 370

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEICKT PQYSYVNGDGTVPSESAKADGFEATERVGVAA+HRGLLKDETVLQLIQK
Sbjct: 371 IEDLSEICKTSPQYSYVNGDGTVPSESAKADGFEATERVGVAATHRGLLKDETVLQLIQK 430

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLGIDEKVSKH TTSKVFDAS+K
Sbjct: 431 WLGIDEKVSKHLTTSKVFDASMK 453

BLAST of Carg26888 vs. ExPASy TrEMBL
Match: A0A0A0LHR5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093800 PE=4 SV=1)

HSP 1 Score: 389.4 bits (999), Expect = 1.0e-104
Identity = 184/203 (90.64%), Postives = 195/203 (96.06%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEML +LGFNWHAQP IQVWQKSSVDG+TSV LKSYGPTDSIALFEEALRNN+
Sbjct: 246 LVECPSIYEMLASLGFNWHAQPHIQVWQKSSVDGETSVNLKSYGPTDSIALFEEALRNNE 305

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFN DILKWATGTRQVID+A+LPDGISFYNIYGTSFDTPFDVCYGSESLP
Sbjct: 306 IKFHGKTIPLPFNFDILKWATGTRQVIDSAKLPDGISFYNIYGTSFDTPFDVCYGSESLP 365

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEIC+TLPQYSYV+GDGTVPSESAKADGFEATERVGVAASHRGLLKD+TVLQ IQK
Sbjct: 366 IEDLSEICQTLPQYSYVDGDGTVPSESAKADGFEATERVGVAASHRGLLKDKTVLQYIQK 425

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLG+++KV KH TTSKV DASLK
Sbjct: 426 WLGVEQKVGKHLTTSKVVDASLK 448

BLAST of Carg26888 vs. ExPASy TrEMBL
Match: A0A5A7U650 (Phospholipase A(1) LCAT3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold419G00130 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.1e-102
Identity = 180/203 (88.67%), Postives = 193/203 (95.07%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEML +LGFNWHAQP IQVWQKSSVDG+TSV+LKSYGPTDSI LF+EALRNN+
Sbjct: 175 LVECPSIYEMLASLGFNWHAQPHIQVWQKSSVDGETSVDLKSYGPTDSIDLFKEALRNNE 234

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFN DILKWATGTRQVID+A+LPDGISFYNIYGTSFDTPFDVCYGSES P
Sbjct: 235 IKFHGKTIPLPFNFDILKWATGTRQVIDSAKLPDGISFYNIYGTSFDTPFDVCYGSESSP 294

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEIC+TLPQYSYV+GDGTVPSESAKADGFEA ERVGVAASHRGLLKD+TV Q IQK
Sbjct: 295 IEDLSEICQTLPQYSYVDGDGTVPSESAKADGFEAIERVGVAASHRGLLKDKTVFQYIQK 354

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLG+++KVSKH TTSKV DASLK
Sbjct: 355 WLGVEQKVSKHLTTSKVVDASLK 377

BLAST of Carg26888 vs. ExPASy TrEMBL
Match: A0A1S3B3Q4 (phospholipase A(1) LCAT3 OS=Cucumis melo OX=3656 GN=LOC103485824 PE=4 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 2.1e-102
Identity = 180/203 (88.67%), Postives = 193/203 (95.07%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSVDGKTSVELKSYGPTDSIALFEEALRNNQ 83
           LVECPSIYEML +LGFNWHAQP IQVWQKSSVDG+TSV+LKSYGPTDSI LF+EALRNN+
Sbjct: 247 LVECPSIYEMLASLGFNWHAQPHIQVWQKSSVDGETSVDLKSYGPTDSIDLFKEALRNNE 306

Query: 84  IKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESLP 143
           IKFHGKTIPLPFN DILKWATGTRQVID+A+LPDGISFYNIYGTSFDTPFDVCYGSES P
Sbjct: 307 IKFHGKTIPLPFNFDILKWATGTRQVIDSAKLPDGISFYNIYGTSFDTPFDVCYGSESSP 366

Query: 144 IEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQK 203
           IEDLSEIC+TLPQYSYV+GDGTVPSESAKADGFEA ERVGVAASHRGLLKD+TV Q IQK
Sbjct: 367 IEDLSEICQTLPQYSYVDGDGTVPSESAKADGFEAIERVGVAASHRGLLKDKTVFQYIQK 426

Query: 204 WLGIDEKVSKHPTTSKVFDASLK 227
           WLG+++KVSKH TTSKV DASLK
Sbjct: 427 WLGVEQKVSKHLTTSKVVDASLK 449

BLAST of Carg26888 vs. TAIR 10
Match: AT3G03310.1 (lecithin:cholesterol acyltransferase 3 )

HSP 1 Score: 258.5 bits (659), Expect = 5.2e-69
Identity = 120/203 (59.11%), Postives = 157/203 (77.34%), Query Frame = 0

Query: 24  LVECPSIYEMLGNLGFNWHAQPQIQVWQKSSV-DGKTSVELKSYGPTDSIALFEEALRNN 83
           LVECPSIYEM+ N  F W  QP+I+VW+K S  D  TSVEL+S+G  +SI LF +AL+NN
Sbjct: 244 LVECPSIYEMMANPDFKWKKQPEIRVWRKKSENDVDTSVELESFGLIESIDLFNDALKNN 303

Query: 84  QIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSFDTPFDVCYGSESL 143
           ++ + G  I LPFN  IL WA  TR++++ A+LPDG+SFYNIYG S +TPFDVCYG+E+ 
Sbjct: 304 ELSYGGNKIALPFNFAILDWAAKTREILNKAQLPDGVSFYNIYGVSLNTPFDVCYGTETS 363

Query: 144 PIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHRGLLKDETVLQLIQ 203
           PI+DLSEIC+T+P+Y+YV+GDGTVP+ESA A  F+A   VGV+ SHRGLL+DE V +LIQ
Sbjct: 364 PIDDLSEICQTMPEYTYVDGDGTVPAESAAAAQFKAVASVGVSGSHRGLLRDERVFELIQ 423

Query: 204 KWLGIDEKVS--KHPTTSKVFDA 224
           +WLG++ K +  KH  T KV D+
Sbjct: 424 QWLGVEPKKAKRKHLRTHKVVDS 446

BLAST of Carg26888 vs. TAIR 10
Match: AT4G19860.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 191.4 bits (485), Expect = 7.7e-49
Identity = 90/200 (45.00%), Postives = 132/200 (66.00%), Query Frame = 0

Query: 13  TSWSTNDIYVQLVECPSIYEMLGNLGFNWHAQPQIQVW-QKSSVD--GKTSVELKSYGPT 72
           + WS + +   L+ECPSIYE++    F W   P +++W +K S D  G + V L+SY   
Sbjct: 241 SKWSMHQL---LIECPSIYELMCCPYFKWELPPVLELWREKESNDGVGTSYVVLESYCSL 300

Query: 73  DSIALFEEALRNNQIKFHGKTIPLPFNLDILKWATGTRQVIDNARLPDGISFYNIYGTSF 132
           +S+ +F ++L NN   + G++I LPFN  I++WA  T+QV+ +A+LP  + FYNIYGT+ 
Sbjct: 301 ESLEVFTKSLSNNTADYCGESIDLPFNWKIMEWAHKTKQVLASAKLPPKVKFYNIYGTNL 360

Query: 133 DTPFDVCYGSESLPIEDLSEICKTLPQYSYVNGDGTVPSESAKADGFEATERVGVAASHR 192
           +TP  VCYG+E +P++DL+ +    P Y  V+GDGTVP ESA ADG EA  RVGV   HR
Sbjct: 361 ETPHSVCYGNEKMPVKDLTNLRYFQPTYICVDGDGTVPMESAMADGLEAVARVGVPGEHR 420

Query: 193 GLLKDETVLQLIQKWLGIDE 210
           G+L D  V ++++KWL + E
Sbjct: 421 GILNDHRVFRMLKKWLNVGE 437

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029921.11.1e-129100.00Phospholipase A(1) LCAT3, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6598962.11.7e-114100.00Phospholipase A(1) LCAT3, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022933151.11.6e-11298.52phospholipase A(1) LCAT3 [Cucurbita moschata][more]
XP_023546771.12.3e-11197.54phospholipase A(1) LCAT3 [Cucurbita pepo subsp. pepo][more]
XP_022973913.11.9e-11096.06phospholipase A(1) LCAT3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q93V617.3e-6859.11Phospholipase A(1) LCAT3 OS=Arabidopsis thaliana OX=3702 GN=LCAT3 PE=1 SV=1[more]
Q71N541.1e-4745.00Lecithin-cholesterol acyltransferase-like 4 OS=Arabidopsis thaliana OX=3702 GN=L... [more]
Match NameE-valueIdentityDescription
A0A6J1EYB87.7e-11398.52phospholipase A(1) LCAT3 OS=Cucurbita moschata OX=3662 GN=LOC111439951 PE=4 SV=1[more]
A0A6J1IEK69.4e-11196.06phospholipase A(1) LCAT3 OS=Cucurbita maxima OX=3661 GN=LOC111472543 PE=4 SV=1[more]
A0A0A0LHR51.0e-10490.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G093800 PE=4 SV=1[more]
A0A5A7U6502.1e-10288.67Phospholipase A(1) LCAT3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol... [more]
A0A1S3B3Q42.1e-10288.67phospholipase A(1) LCAT3 OS=Cucumis melo OX=3656 GN=LOC103485824 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G03310.15.2e-6959.11lecithin:cholesterol acyltransferase 3 [more]
AT4G19860.17.7e-4945.00alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 44..218
e-value: 2.1E-9
score: 39.2
NoneNo IPR availablePANTHERPTHR11440LECITHIN-CHOLESTEROL ACYLTRANSFERASE-RELATEDcoord: 24..223
NoneNo IPR availablePANTHERPTHR11440:SF53PHOSPHOLIPASE A(1) LCAT3coord: 24..223

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26888-RACarg26888-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0008374 O-acyltransferase activity