Carg26883 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26883
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	adenylate isopentenyltransferase-like
Location	Carg_Chr15: 4966952 .. 4967932 (-)
RNA-Seq Expression	Carg26883
Synteny	Carg26883

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGGCCCCGATCCCCACTTGTTCTCTCCTCCTCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTTCGCTCCCACGCTGCGGCCGTCATCTCCGGCGTAATCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCACGGCGGATTTCGATCCAAGCCACGACGTCTTCGGCTGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGAGGGTTGATGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACGAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGATGCTGGGGTCGGAAGCGGCGGAGAAACGCTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGCAGTAG

mRNA sequence

ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGGCCCCGATCCCCACTTGTTCTCTCCTCCTCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTTCGCTCCCACGCTGCGGCCGTCATCTCCGGCGTAATCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCACGGCGGATTTCGATCCAAGCCACGACGTCTTCGGCTGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGAGGGTTGATGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACGAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGATGCTGGGGTCGGAAGCGGCGGAGAAACGCTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGCAGTAG

Coding sequence (CDS)

ATGACACTTTCCCTCTCCTTCTCCCATTTTAATGCCCCTTTTTCCTGTTCTTCCCGCCGGCCCCGATCCCCACTTGTTCTCTCCTCCTCCTCCCCCTCCCATAATCTCATCCTCCTCCTCGGCGCCACCGGCTCCGGCAAGTCCTCCCTCTCCATTGACCTCGCCTCCCGCTTCCCCTCCGAGATTATTAATTCTGACAAAATGCAACTCTACACAGGCCTCGACATCACCACTAACAAGCTCCCCCTCTCCGACCGCCGCGCCGTCCCCCACCATCTTCTCGGCGACTTCGATCCTCTCGCCGGCGAAGTCTCCGCATCAGCATTTCGCTCCCACGCTGCGGCCGTCATCTCCGGCGTAATCTCCCGAGGGAACCTTCCTCTCGTCGTCGGCGGTTCCAACTCCTTCATTCACGCGCTTGTCACGGCGGATTTCGATCCAAGCCACGACGTCTTCGGCTGCTCTCGGAATCGGGTGTCTACGGAGTTCCGGTACCGGTGCTGCTTTCTGTGGGTGGACGTGTCGTTTCCGGTTCTGAAACGGTACTTGTCGAAGAGGGTTGATGAGATGTTAGAGGCCGGGATGGTAAGGGAGTTGGCGGAGTTTTACGACCCGGAAATTGCCGATTCAGAGACCCGAGTGGGGCTGAGGAAGGCGATCGGAGTGGCGGAATTCGAGGAGTTTTTCCGGCGGCATCACCCGAAAGGGAGAGAGTACAAAGAGGGGAGGGATCCAGTGGTGGCGAGCGCATACAAAGAGGCGGTAAGGAAAATAAAGGAAAACACGTGGAAGCTAACGAAGAGGCAGTTAGGGAAGATCGGACGGCTGAGAAGAGCGGGGTGGGACCTGAAAAGAGTGGACGCGACAGCGGCGGTTGTGGCGATGCTGGGGTCGGAAGCGGCGGAGAAACGCTCAGAAATATGGGAGGCAGAGGTAGTGGAAGCAAGCGTAAAGATTGTGAAGAGGTTCATTGCGCAGTAG

Protein sequence

MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIAQ

Homology

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BLAST of Carg26883 vs. NCBI nr
Match: KAG6579205.1 (hypothetical protein SDJN03_23653, partial [Cucurbita argyrosperma subsp. sororia] >KAG7016719.1 hypothetical protein SDJN02_21829, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 635.2 bits (1637), Expect = 3.1e-178
Identity = 326/326 (100.00%), Postives = 326/326 (100.00%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS
Sbjct: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV
Sbjct: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK
Sbjct: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA
Sbjct: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWEAEVVEASVKIVKRFIAQ
Sbjct: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 326

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BLAST of Carg26883 vs. NCBI nr
Match: XP_022939170.1 (adenylate isopentenyltransferase-like [Cucurbita moschata])

HSP 1 Score: 624.8 bits (1610), Expect = 4.2e-175
Identity = 324/331 (97.89%), Postives = 325/331 (98.19%), Query Frame = 0


Query: 1   MTLSLSF------SHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 60
           MTLSLSF      SHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 102

Query: 61  ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 120
           ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA
Sbjct: 103 ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 162

Query: 121 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 180
           AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV
Sbjct: 163 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 222

Query: 181 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 240
           SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP
Sbjct: 223 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 282

Query: 241 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 300
           KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA
Sbjct: 283 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 342

Query: 301 MLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 326
           +LGSEAAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 343 VLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 373

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BLAST of Carg26883 vs. NCBI nr
Match: XP_023551170.1 (adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 618.6 bits (1594), Expect = 3.0e-173
Identity = 320/335 (95.52%), Postives = 324/335 (96.72%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSS----------SPSHNLILLLGATGSGKSSL 60
           MTLSLSFSHFNAPFSCSSRRPRSPLVLSSS          SPSHNLILLLGATGSGKSSL
Sbjct: 52  MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSSSSSSSSSPSPSHNLILLLGATGSGKSSL 111

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR
Sbjct: 112 SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 171

Query: 121 SHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFL 180
           SHAAAVISGV+SRGNLPLVVGGSNSFIHALV ADFDP+HDVFGCSRNRVSTEFRYRCCFL
Sbjct: 172 SHAAAVISGVVSRGNLPLVVGGSNSFIHALVAADFDPTHDVFGCSRNRVSTEFRYRCCFL 231

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR
Sbjct: 232 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 291

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA
Sbjct: 292 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 351

Query: 301 AVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 326
           AVVA+LGS+AAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 352 AVVAVLGSDAAEKRSEIWEAEVVEASVKIVKRFIA 386

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BLAST of Carg26883 vs. NCBI nr
Match: XP_008445640.1 (PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa] >TYK08909.1 adenylate isopentenyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 515.0 bits (1325), Expect = 4.7e-142
Identity = 272/326 (83.44%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSL FSH + P    SRRPR PLVL SS+PS +L+LLLGATGSGKSSLSIDLA+RFPS
Sbjct: 1   MTLSLPFSH-SFPL---SRRPRCPLVL-SSAPSDSLLLLLGATGSGKSSLSIDLAARFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFRSHA+AVISGV
Sbjct: 61  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSFIHALV ADFD + DVF  SRNRVS EFRYRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FRRHHPKGR+Y+
Sbjct: 181 RYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYR 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA+L SEA
Sbjct: 241 EG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEA 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWE +VVE SVKIVK FI +
Sbjct: 301 AEKRSEIWETQVVEPSVKIVKGFIQE 320

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BLAST of Carg26883 vs. NCBI nr
Match: XP_004150914.1 (adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical protein Csa_005709 [Cucumis sativus])

HSP 1 Score: 512.7 bits (1319), Expect = 2.3e-141
Identity = 270/326 (82.82%), Postives = 292/326 (89.57%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSL FSH        SRRPR PL+L SS+PS +L+LLLGATGSGKSSLSIDLA+RFPS
Sbjct: 1   MTLSLPFSH----SFLLSRRPRCPLIL-SSAPSDSLLLLLGATGSGKSSLSIDLAARFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFRSHA+AVISGV
Sbjct: 61  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSF+HALV ADFD + DVF  SRNRVSTEF YRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FRRHHPKGR+Y+
Sbjct: 181 RYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYR 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EG DP+ A A++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA+L SE 
Sbjct: 241 EG-DPLQARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEV 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWE +VVE SVKIVK FI +
Sbjct: 301 AEKRSEIWETQVVEPSVKIVKGFIQE 320

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BLAST of Carg26883 vs. ExPASy Swiss-Prot
Match: Q5GHF7 (Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 4.7e-84
Identity = 173/296 (58.45%), Postives = 214/296 (72.30%), Query Frame = 0


Query: 36  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 95
           L++L+GATG+GKS LSIDLA+ FP E+INSDKMQ+Y GLDITTNK+ + DR  VPHHLLG
Sbjct: 32  LLVLMGATGTGKSRLSIDLAAHFPLEVINSDKMQVYKGLDITTNKISVPDRGGVPHHLLG 91

Query: 96  DFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPS-HDVF-G 155
           + DP  GE++ + FRS A   +S +  R  LP++VGGSNSFIHAL+   FD S   VF  
Sbjct: 92  EVDPARGELTPADFRSLAGKAVSEITGRRKLPVLVGGSNSFIHALLVDRFDSSGPGVFEE 151

Query: 156 CSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPE----IAD 215
            S + VS+E RY CCFLWVDVS  VL  YL+KRVD+MLE GM  ELAEFY PE      D
Sbjct: 152 GSHSVVSSELRYDCCFLWVDVSVKVLTDYLAKRVDDMLELGMFDELAEFYSPEDEDHDED 211

Query: 216 SETRVGLRKAIGVAEFEEFFRRHHPKGREYKE-GRDPVVASAYKEAVRKIKENTWKLTKR 275
           S TR GLRKAIGV EF+ +F +  P   E ++ GRD V   A++EAVR IKENT  L KR
Sbjct: 212 SATRTGLRKAIGVPEFDRYFEKFRPGDVEGEDPGRDRVRRGAFEEAVRAIKENTCHLAKR 271

Query: 276 QLGKIGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFI 325
           Q+GKI RL+ AGWDL+R+DAT +  A + S++ EK +EIWE +V+E SVKIV RF+
Sbjct: 272 QIGKILRLKGAGWDLRRLDATESFRAAMTSDSGEKCTEIWEKQVLEPSVKIVSRFL 327

[Close]

BLAST of Carg26883 vs. ExPASy Swiss-Prot
Match: Q94ID3 (Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT1 PE=1 SV=2)

HSP 1 Score: 304.7 bits (779), Expect = 1.3e-81
Identity = 167/338 (49.41%), Postives = 227/338 (67.16%), Query Frame = 0


Query: 2   TLSLSFSHFNAPFS-----CSSRRPRSPLVLS-------SSSPSHNLILLLGATGSGKSS 61
           T S SFS  ++  S        RR   PLV S       S +    ++++LGATG+GKS 
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSR 80

Query: 62  LSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAF 121
           LS+DLA+RFPSEIINSDK+Q+Y GL+ITTN++ L DRR VPHHLLG  +P  GE++A  F
Sbjct: 81  LSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEF 140

Query: 122 RSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCF 181
           RS A+ V+  + SR  +P++ GGSNSF+HAL+   FDP  D F      +S++ RY CCF
Sbjct: 141 RSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCF 200

Query: 182 LWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFF 241
           +WVDVS  VL  YL +RVDEM+++GM  EL+ FYDP  +  ETR G+RKAIGV EF+ +F
Sbjct: 201 IWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYF 260

Query: 242 RRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDAT 301
           + + P+ +  K   D +  +AY +AV  IK NTW L KRQ+ KI  L+ AGW+++RVDAT
Sbjct: 261 KEYPPEKKMIK--WDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDAT 320

Query: 302 AAVVAMLGSEAAEKR-SEIWEAEVVEASVKIVKRFIAQ 327
           A+  A++   ++EK+  E WE +V+E SVKIVKR + Q
Sbjct: 321 ASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQ 356

[Close]

BLAST of Carg26883 vs. ExPASy Swiss-Prot
Match: Q9LJL4 (Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT8 PE=2 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 5.7e-74
Identity = 144/302 (47.68%), Postives = 200/302 (66.23%), Query Frame = 0


Query: 26  VLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSD 85
           V    S    +++++GATGSGKS LSIDLA+RF  EI+NSDK+Q Y GL +TTN++ + +
Sbjct: 35  VCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILE 94

Query: 86  RRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADF 145
           R  VPHHLLG+  P   E++ S FRS A+  IS + +RGNLP++ GGSNSFIHAL+   F
Sbjct: 95  RCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRF 154

Query: 146 DPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDP 205
           DP    F  S   +S+  RY CCFLWVDVS  VL  YLSKRVD+M+E+GM  ELA FYDP
Sbjct: 155 DPKTYPFS-SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDP 214

Query: 206 EIADSETRV-GLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWK 265
             + S  R  G+ K IG+ EF+ +F  + P+ ++     D     AY EAV++IKENTW+
Sbjct: 215 RYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWR 274

Query: 266 LTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFI 325
           L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+VKRF+
Sbjct: 275 LAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 327

Query: 326 AQ 327
            +
Sbjct: 335 VK 327

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BLAST of Carg26883 vs. ExPASy Swiss-Prot
Match: Q9C6L1 (Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPT6 PE=2 SV=1)

HSP 1 Score: 273.1 bits (697), Expect = 4.1e-72
Identity = 150/298 (50.34%), Postives = 206/298 (69.13%), Query Frame = 0


Query: 36  LILLLGATGSGKSSLSIDLASR-FPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLL 95
           ++L+ G TG+GKS LS+DLA+R FP+EIINSDKMQ+Y G +I TN +PL ++  VPHHLL
Sbjct: 47  VVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLL 106

Query: 96  GDFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVF-- 155
           G F P  GE++ + FRS A   IS +IS   LP+VVGGSNSF HAL+   FDP  D F  
Sbjct: 107 GQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSP 166

Query: 156 GCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSET 215
           G S + + ++ RY+CC LWVDV  PVL ++L  RVD+M+E+G+V +LAE YDP + DS  
Sbjct: 167 GSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGR 226

Query: 216 RVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGK 275
           R+G+RK IGV EF+ +F R +PK  + K   D    +AY+E V+ +KE T +L K+Q  K
Sbjct: 227 RLGVRKTIGVEEFDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEK 286

Query: 276 IGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRS----EIWEAEVVEASVKIVKRFIAQ 327
           I +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK+F+ +
Sbjct: 287 IMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Carg26883 vs. ExPASy Swiss-Prot
Match: Q9SB60 (Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 9.1e-72
Identity = 145/296 (48.99%), Postives = 201/296 (67.91%), Query Frame = 0


Query: 36  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 95
           +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  + DRR VPHHLLG
Sbjct: 7   MVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLLG 66

Query: 96  DFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCS 155
           + +P AGEV+A+ FR  AA  IS +  R  LP++ GGSNS+IHAL+   +DP +  F   
Sbjct: 67  ELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSDH 126

Query: 156 RNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVG 215
           +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  E  +G
Sbjct: 127 KGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP-LG 186

Query: 216 LRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGR 275
           + KAIGV EF+++ + +  K     +  DP+   AY++AVR IKENT++LTK Q+ KI +
Sbjct: 187 IWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 246

Query: 276 LRRAGWDLKRVDATAAV-----VAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIAQ 327
           LR AGWD+K+VDATA+       A  G   AE + +IW  EV+E  VKIV+  + Q
Sbjct: 247 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Carg26883 vs. ExPASy TrEMBL
Match: A0A6J1FLX4 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445165 PE=4 SV=1)

HSP 1 Score: 624.8 bits (1610), Expect = 2.1e-175
Identity = 324/331 (97.89%), Postives = 325/331 (98.19%), Query Frame = 0


Query: 1   MTLSLSF------SHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 60
           MTLSLSF      SHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL
Sbjct: 43  MTLSLSFSHSSLHSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDL 102

Query: 61  ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 120
           ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA
Sbjct: 103 ASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAA 162

Query: 121 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 180
           AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV
Sbjct: 163 AVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDV 222

Query: 181 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 240
           SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP
Sbjct: 223 SFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHP 282

Query: 241 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 300
           KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA
Sbjct: 283 KGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVA 342

Query: 301 MLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 326
           +LGSEAAEKRSEIWEAEVVEASVKIVKRFIA
Sbjct: 343 VLGSEAAEKRSEIWEAEVVEASVKIVKRFIA 373

[Close]

BLAST of Carg26883 vs. ExPASy TrEMBL
Match: A0A5D3CF13 (Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold383G00180 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.3e-142
Identity = 272/326 (83.44%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSL FSH + P    SRRPR PLVL SS+PS +L+LLLGATGSGKSSLSIDLA+RFPS
Sbjct: 1   MTLSLPFSH-SFPL---SRRPRCPLVL-SSAPSDSLLLLLGATGSGKSSLSIDLAARFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFRSHA+AVISGV
Sbjct: 61  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSFIHALV ADFD + DVF  SRNRVS EFRYRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FRRHHPKGR+Y+
Sbjct: 181 RYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYR 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA+L SEA
Sbjct: 241 EG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEA 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWE +VVE SVKIVK FI +
Sbjct: 301 AEKRSEIWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Carg26883 vs. ExPASy TrEMBL
Match: A0A1S3BD73 (adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE=4 SV=1)

HSP 1 Score: 515.0 bits (1325), Expect = 2.3e-142
Identity = 272/326 (83.44%), Postives = 295/326 (90.49%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSL FSH + P    SRRPR PLVL SS+PS +L+LLLGATGSGKSSLSIDLA+RFPS
Sbjct: 1   MTLSLPFSH-SFPL---SRRPRCPLVL-SSAPSDSLLLLLGATGSGKSSLSIDLAARFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGD+DPLAGEVS +AFRSHA+AVISGV
Sbjct: 61  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDYDPLAGEVSPAAFRSHASAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSFIHALV ADFD + DVF  SRNRVS EFRYRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFIHALVAADFDDTQDVFCGSRNRVSAEFRYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEML+ GM+ ELA+FYDPE+AD ETRVGLRKAIGV EFEE+FRRHHPKGR+Y+
Sbjct: 181 RYLSKRVDEMLDGGMLEELAQFYDPEMADLETRVGLRKAIGVPEFEEYFRRHHPKGRDYR 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EG DP+ ASA++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA+L SEA
Sbjct: 241 EG-DPLQASAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEA 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWE +VVE SVKIVK FI +
Sbjct: 301 AEKRSEIWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Carg26883 vs. ExPASy TrEMBL
Match: A0A0A0K943 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.1e-141
Identity = 270/326 (82.82%), Postives = 292/326 (89.57%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           MTLSL FSH        SRRPR PL+L SS+PS +L+LLLGATGSGKSSLSIDLA+RFPS
Sbjct: 1   MTLSLPFSH----SFLLSRRPRCPLIL-SSAPSDSLLLLLGATGSGKSSLSIDLAARFPS 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDKMQLY GLDITTNKLPLS RRAVPHHLLGDFDPLAGEVS +AFRSHA+AVISGV
Sbjct: 61  EIINSDKMQLYRGLDITTNKLPLSHRRAVPHHLLGDFDPLAGEVSPAAFRSHASAVISGV 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
           ISRGNLPLVVGGSNSF+HALV ADFD + DVF  SRNRVSTEF YRCCFLWVDVSFPVLK
Sbjct: 121 ISRGNLPLVVGGSNSFVHALVAADFDATQDVFCGSRNRVSTEFLYRCCFLWVDVSFPVLK 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
           RYLSKRVDEML+ GMV ELA+FYDPE+ADSETRVGLRKAIGV EFEE+FRRHHPKGR+Y+
Sbjct: 181 RYLSKRVDEMLDGGMVEELAKFYDPEMADSETRVGLRKAIGVPEFEEYFRRHHPKGRDYR 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEA 300
           EG DP+ A A++EAVRKIKENTW+LTKRQL KIGRLRRAGWDLKRVDATAAVVA+L SE 
Sbjct: 241 EG-DPLQARAFEEAVRKIKENTWQLTKRQLWKIGRLRRAGWDLKRVDATAAVVAVLASEV 300

Query: 301 AEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AEKRSEIWE +VVE SVKIVK FI +
Sbjct: 301 AEKRSEIWETQVVEPSVKIVKGFIQE 320

[Close]

BLAST of Carg26883 vs. ExPASy TrEMBL
Match: A0A6J1HF40 (adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463593 PE=4 SV=1)

HSP 1 Score: 498.8 bits (1283), Expect = 1.7e-137
Identity = 265/336 (78.87%), Postives = 291/336 (86.61%), Query Frame = 0


Query: 1   MTLSLSFSHFNAP----------FSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSL 60
           MTLSL FSH +            F   SRR + P +L SS PS NL+LLLGATGSGKSSL
Sbjct: 1   MTLSLPFSHSSLHYHHHHHPFPLFLSPSRRFQWPRML-SSLPSDNLLLLLGATGSGKSSL 60

Query: 61  SIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFR 120
           SID+A+RFPSEIINSDKMQLY GLD+TTNKLP SDRR VPHHLLGDFDPLAGEVSA+AFR
Sbjct: 61  SIDIAARFPSEIINSDKMQLYRGLDVTTNKLPFSDRRGVPHHLLGDFDPLAGEVSAAAFR 120

Query: 121 SHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFL 180
           S A+AVISGV+SRGNLP+VVGGSNS+IHALVTA FD  +DVF    NRVS EFRYRCCFL
Sbjct: 121 SQASAVISGVVSRGNLPVVVGGSNSYIHALVTAGFDQDYDVFDGPLNRVSAEFRYRCCFL 180

Query: 181 WVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFR 240
           WVDVSFPVLK+YLSKRVD+MLEAGMV ELAEFYDPE+A++E RVGLRKAIGV EFEEFFR
Sbjct: 181 WVDVSFPVLKQYLSKRVDDMLEAGMVGELAEFYDPEMAETEPRVGLRKAIGVPEFEEFFR 240

Query: 241 RHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDATA 300
           R+HPKGREY+EG DP+ A AY+EAVRKIKENTW+LTKRQLGKIGRLR AGWDLKRVDATA
Sbjct: 241 RYHPKGREYREG-DPLRAGAYEEAVRKIKENTWQLTKRQLGKIGRLRMAGWDLKRVDATA 300

Query: 301 AVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIAQ 327
           AVVA+L SEAAEKRSEIWEA+VVE SVKIVKRFI +
Sbjct: 301 AVVAVLASEAAEKRSEIWEAQVVEPSVKIVKRFIEE 334

[Close]

BLAST of Carg26883 vs. TAIR 10
Match: AT1G68460.1 (isopentenyltransferase 1 )

HSP 1 Score: 304.7 bits (779), Expect = 9.0e-83
Identity = 167/338 (49.41%), Postives = 227/338 (67.16%), Query Frame = 0


Query: 2   TLSLSFSHFNAPFS-----CSSRRPRSPLVLS-------SSSPSHNLILLLGATGSGKSS 61
           T S SFS  ++  S        RR   PLV S       S +    ++++LGATG+GKS 
Sbjct: 21  TTSPSFSSHSSSSSSLLSFTKRRRKHQPLVSSIRMEQSRSRNRKDKVVVILGATGAGKSR 80

Query: 62  LSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAF 121
           LS+DLA+RFPSEIINSDK+Q+Y GL+ITTN++ L DRR VPHHLLG  +P  GE++A  F
Sbjct: 81  LSVDLATRFPSEIINSDKIQVYEGLEITTNQITLQDRRGVPHHLLGVINPEHGELTAGEF 140

Query: 122 RSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCF 181
           RS A+ V+  + SR  +P++ GGSNSF+HAL+   FDP  D F      +S++ RY CCF
Sbjct: 141 RSAASNVVKEITSRQKVPIIAGGSNSFVHALLAQRFDPKFDPFSSGSCLISSDLRYECCF 200

Query: 182 LWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFF 241
           +WVDVS  VL  YL +RVDEM+++GM  EL+ FYDP  +  ETR G+RKAIGV EF+ +F
Sbjct: 201 IWVDVSETVLYEYLLRRVDEMMDSGMFEELSRFYDPVKSGLETRFGIRKAIGVPEFDGYF 260

Query: 242 RRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGWDLKRVDAT 301
           + + P+ +  K   D +  +AY +AV  IK NTW L KRQ+ KI  L+ AGW+++RVDAT
Sbjct: 261 KEYPPEKKMIK--WDALRKAAYDKAVDDIKRNTWTLAKRQVKKIEMLKDAGWEIERVDAT 320

Query: 302 AAVVAMLGSEAAEKR-SEIWEAEVVEASVKIVKRFIAQ 327
           A+  A++   ++EK+  E WE +V+E SVKIVKR + Q
Sbjct: 321 ASFKAVMMKSSSEKKWRENWEEQVLEPSVKIVKRHLVQ 356

[Close]

BLAST of Carg26883 vs. TAIR 10
Match: AT3G19160.1 (ATP/ADP isopentenyltransferases )

HSP 1 Score: 279.3 bits (713), Expect = 4.1e-75
Identity = 144/302 (47.68%), Postives = 200/302 (66.23%), Query Frame = 0


Query: 26  VLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSD 85
           V    S    +++++GATGSGKS LSIDLA+RF  EI+NSDK+Q Y GL +TTN++ + +
Sbjct: 35  VCMEQSYKQKVVVIMGATGSGKSCLSIDLATRFSGEIVNSDKIQFYDGLKVTTNQMSILE 94

Query: 86  RRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADF 145
           R  VPHHLLG+  P   E++ S FRS A+  IS + +RGNLP++ GGSNSFIHAL+   F
Sbjct: 95  RCGVPHHLLGELPPDDSELTTSEFRSLASRSISEITARGNLPIIAGGSNSFIHALLVDRF 154

Query: 146 DPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDP 205
           DP    F  S   +S+  RY CCFLWVDVS  VL  YLSKRVD+M+E+GM  ELA FYDP
Sbjct: 155 DPKTYPFS-SETSISSGLRYECCFLWVDVSVSVLFEYLSKRVDQMMESGMFEELAGFYDP 214

Query: 206 EIADSETRV-GLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWK 265
             + S  R  G+ K IG+ EF+ +F  + P+ ++     D     AY EAV++IKENTW+
Sbjct: 215 RYSGSAIRAHGIHKTIGIPEFDRYFSLYPPERKQKMSEWDQARKGAYDEAVQEIKENTWR 274

Query: 266 LTKRQLGKIGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRSEIWEAEVVEASVKIVKRFI 325
           L K+Q+ +I +L+ +GWD++R+DAT +              EIW+  V++ S+K+VKRF+
Sbjct: 275 LAKKQIERIMKLKSSGWDIQRLDATPSF--------GRSSREIWDNTVLDESIKVVKRFL 327

Query: 326 AQ 327
            +
Sbjct: 335 VK 327

[Close]

BLAST of Carg26883 vs. TAIR 10
Match: AT1G25410.1 (isopentenyltransferase 6 )

HSP 1 Score: 273.1 bits (697), Expect = 2.9e-73
Identity = 150/298 (50.34%), Postives = 206/298 (69.13%), Query Frame = 0


Query: 36  LILLLGATGSGKSSLSIDLASR-FPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLL 95
           ++L+ G TG+GKS LS+DLA+R FP+EIINSDKMQ+Y G +I TN +PL ++  VPHHLL
Sbjct: 47  VVLITGTTGTGKSRLSVDLATRFFPAEIINSDKMQIYKGFEIVTNLIPLHEQGGVPHHLL 106

Query: 96  GDFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVF-- 155
           G F P  GE++ + FRS A   IS +IS   LP+VVGGSNSF HAL+   FDP  D F  
Sbjct: 107 GQFHPQDGELTPAEFRSLATLSISKLISSKKLPIVVGGSNSFNHALLAERFDPDIDPFSP 166

Query: 156 GCSRNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSET 215
           G S + + ++ RY+CC LWVDV  PVL ++L  RVD+M+E+G+V +LAE YDP + DS  
Sbjct: 167 GSSLSTICSDLRYKCCILWVDVLEPVLFQHLCNRVDQMIESGLVEQLAELYDP-VVDSGR 226

Query: 216 RVGLRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGK 275
           R+G+RK IGV EF+ +F R +PK  + K   D    +AY+E V+ +KE T +L K+Q  K
Sbjct: 227 RLGVRKTIGVEEFDRYF-RVYPKEMD-KGIWDLARKAAYEETVKGMKERTCRLVKKQKEK 286

Query: 276 IGRLRRAGWDLKRVDATAAVVAMLGSEAAEKRS----EIWEAEVVEASVKIVKRFIAQ 327
           I +L R GW++KR+DATAA++A L    A+       EIWE  +V+ SV+IVK+F+ +
Sbjct: 287 IMKLIRGGWEIKRLDATAAIMAELNQSTAKGEGKNGREIWEKHIVDESVEIVKKFLLE 341

[Close]

BLAST of Carg26883 vs. TAIR 10
Match: AT4G24650.1 (isopentenyltransferase 4 )

HSP 1 Score: 271.9 bits (694), Expect = 6.5e-73
Identity = 145/296 (48.99%), Postives = 201/296 (67.91%), Query Frame = 0


Query: 36  LILLLGATGSGKSSLSIDLASRFPSEIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLG 95
           +++++GATGSGKSSLS+DLA  F +EIINSDKMQ Y GL ITTN+  + DRR VPHHLLG
Sbjct: 7   MVVIMGATGSGKSSLSVDLALHFKAEIINSDKMQFYDGLKITTNQSTIEDRRGVPHHLLG 66

Query: 96  DFDPLAGEVSASAFRSHAAAVISGVISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCS 155
           + +P AGEV+A+ FR  AA  IS +  R  LP++ GGSNS+IHAL+   +DP +  F   
Sbjct: 67  ELNPEAGEVTAAEFRVMAAEAISEITQRKKLPILAGGSNSYIHALLAKSYDPENYPFSDH 126

Query: 156 RNRVSTEFRYRCCFLWVDVSFPVLKRYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVG 215
           +  + +E +Y CCF+W+DV   VL  YLS R+D M+++GM  E+AEF+  + A  E  +G
Sbjct: 127 KGSICSELKYDCCFIWIDVDQSVLFEYLSLRLDLMMKSGMFEEIAEFHRSKKAPKEP-LG 186

Query: 216 LRKAIGVAEFEEFFRRHHPKGREYKEGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGR 275
           + KAIGV EF+++ + +  K     +  DP+   AY++AVR IKENT++LTK Q+ KI +
Sbjct: 187 IWKAIGVQEFDDYLKMY--KWDNDMDKWDPMRKEAYEKAVRAIKENTFQLTKDQITKINK 246

Query: 276 LRRAGWDLKRVDATAAV-----VAMLGSEAAEKRSEIWEAEVVEASVKIVKRFIAQ 327
           LR AGWD+K+VDATA+       A  G   AE + +IW  EV+E  VKIV+  + Q
Sbjct: 247 LRNAGWDIKKVDATASFREAIRAAKEGEGVAEMQRKIWNKEVLEPCVKIVRSHLDQ 299

[Close]

BLAST of Carg26883 vs. TAIR 10
Match: AT3G23630.1 (isopentenyltransferase 7 )

HSP 1 Score: 202.6 bits (514), Expect = 4.8e-52
Identity = 135/325 (41.54%), Postives = 180/325 (55.38%), Query Frame = 0


Query: 1   MTLSLSFSHFNAPFSCSSRRPRSPLVLSSSSPSHNLILLLGATGSGKSSLSIDLASRFPS 60
           M  S+S      P  C   +     V S   P   +I ++GATGSGKS L+IDLA+RF  
Sbjct: 1   MKFSISSLKQVQPILCFKNKLSKVNVNSFLHPKEKVIFVMGATGSGKSRLAIDLATRFQG 60

Query: 61  EIINSDKMQLYTGLDITTNKLPLSDRRAVPHHLLGDFDPLAGEVSASAFRSHAAAVISGV 120
           EIINSDK+QLY GLD+ TNK+   + R VPHHLLG FD  AG ++A+ +   A+  IS +
Sbjct: 61  EIINSDKIQLYKGLDVLTNKVTPKECRGVPHHLLGVFDSEAGNLTATQYSRLASQAISKL 120

Query: 121 ISRGNLPLVVGGSNSFIHALVTADFDPSHDVFGCSRNRVSTEFRYRCCFLWVDVSFPVLK 180
            +   LP+V GGSNS+I ALV      +H       N       Y CCF+WVDVS PVL 
Sbjct: 121 SANNKLPIVAGGSNSYIEALV------NHSSGFLLNN-------YDCCFIWVDVSLPVLN 180

Query: 181 RYLSKRVDEMLEAGMVRELAEFYDPEIADSETRVGLRKAIGVAEFEEFFRRHHPKGREYK 240
            ++SKRVD M+EAG++ E+ E ++P+   +   VG+R+AIGV E  E+ R      R  K
Sbjct: 181 SFVSKRVDRMMEAGLLEEVREVFNPK---ANYSVGIRRAIGVPELHEYLRNESLVDRATK 240

Query: 241 EGRDPVVASAYKEAVRKIKENTWKLTKRQLGKIGRLRRAGW--DLKRVDATAAVVAMLGS 300
                  +     AV+ IK+NT  L  RQL KI RL +  W   + RVDAT   +     
Sbjct: 241 -------SKMLDVAVKNIKKNTEILACRQLKKIQRLHKK-WKMSMHRVDATEVFL----K 297

Query: 301 EAAEKRSEIWEAEVVEASVKIVKRF 324
              E++ E WE  V   S +IV +F
Sbjct: 301 RNVEEQDEAWENLVARPSERIVDKF 297

The following BLAST results are available for this feature:

BLAST of Carg26883 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6579205.1	3.1e-178	100.00	hypothetical protein SDJN03_23653, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022939170.1	4.2e-175	97.89	adenylate isopentenyltransferase-like [Cucurbita moschata]	[more]
XP_023551170.1	3.0e-173	95.52	adenylate isopentenyltransferase-like [Cucurbita pepo subsp. pepo]	[more]
XP_008445640.1	4.7e-142	83.44	PREDICTED: adenylate isopentenyltransferase-like [Cucumis melo] >KAA0049650.1 ad...	[more]
XP_004150914.1	2.3e-141	82.82	adenylate isopentenyltransferase [Cucumis sativus] >KGN45943.1 hypothetical prot...	[more]

BLAST of Carg26883 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5GHF7	4.7e-84	58.45	Adenylate isopentenyltransferase OS=Humulus lupulus OX=3486 PE=1 SV=1	[more]
Q94ID3	1.3e-81	49.41	Adenylate isopentenyltransferase 1, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9LJL4	5.7e-74	47.68	Adenylate isopentenyltransferase 8, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9C6L1	4.1e-72	50.34	Adenylate isopentenyltransferase 6, chloroplastic OS=Arabidopsis thaliana OX=370...	[more]
Q9SB60	9.1e-72	48.99	Adenylate isopentenyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=IPT4 PE=1 ...	[more]

BLAST of Carg26883 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FLX4	2.1e-175	97.89	adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111445...	[more]
A0A5D3CF13	2.3e-142	83.44	Adenylate isopentenyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=...	[more]
A0A1S3BD73	2.3e-142	83.44	adenylate isopentenyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103488599 PE...	[more]
A0A0A0K943	1.1e-141	82.82	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G030440 PE=4 SV=1	[more]
A0A6J1HF40	1.7e-137	78.87	adenylate isopentenyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111463...	[more]

BLAST of Carg26883 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68460.1	9.0e-83	49.41	isopentenyltransferase 1	[more]
AT3G19160.1	4.1e-75	47.68	ATP/ADP isopentenyltransferases	[more]
AT1G25410.1	2.9e-73	50.34	isopentenyltransferase 6	[more]
AT4G24650.1	6.5e-73	48.99	isopentenyltransferase 4	[more]
AT3G23630.1	4.8e-52	41.54	isopentenyltransferase 7	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF01715	IPPT	coord: 161..270 e-value: 2.1E-12 score: 47.3 coord: 69..143 e-value: 4.7E-16 score: 59.2
None	No IPR available	GENE3D	1.10.287.890	Crystal structure of tRNA isopentenylpyrophosphate transferase (bh2366) domain	coord: 176..283 e-value: 3.5E-121 score: 405.5
None	No IPR available	PANTHER	PTHR11088:SF65	ADENYLATE ISOPENTENYLTRANSFERASE 4-RELATED	coord: 29..324
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 35..324 e-value: 3.5E-121 score: 405.5
IPR027417	P-loop containing nucleoside triphosphate hydrolase	SUPERFAMILY	52540	P-loop containing nucleoside triphosphate hydrolases	coord: 32..210
IPR039657	Dimethylallyltransferase	PANTHER	PTHR11088	TRNA DIMETHYLALLYLTRANSFERASE	coord: 29..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26883-RA	Carg26883-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006400	tRNA modification
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0009824	AMP dimethylallyltransferase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0052381	tRNA dimethylallyltransferase activity