Carg26781 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26781
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein kinase domain-containing protein
LocationCarg_Chr15: 6693723 .. 6697316 (+)
RNA-Seq ExpressionCarg26781
SyntenyCarg26781
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACACCACTGTAGTAAAAGCGTTTCTGATATCAGTAACCATGCTGTAATTTCCGGTGGCAGCCATCCAACCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTGGAATAGCGCCGTCGCCAGCCAGAACTCCGAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCACCAGCAAAGCCCATCATGGCCATTCTCCGGCGGAAATCACGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTAGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGATGAACTTTGGAGCGAAGTTCGAGCTCGGGAAGGAGGTGGGGAGGGGCCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAAGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAATCTAAGGTTGGTTTAGTTCAATACTCATTTCTTCACCTCAATCTGTTCCTTCGTTTTGTTCATCTAAAACGTGAGATCCTAATCTAAATCTGCTATAAAATCCATTTACATAAGTGGGAACGAAAGACTTACATTATTTTGATGCCAACTCGCTGCTCGGACTTATTTGGTTTGGCAGATGACATCGACTGTTGGAATCGAAGATGTTCGCCGGGAGGTAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGTGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAGTATGTGCTTGTAGATATCTGTTTTTGCCAATAGCCCTGCATTTTCTGATAGCTCAATCAAGTTAATATCAACCCTTTTTCTGAATTTGGTATCAGATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGGTAAAAATGGTTTGATTCATTTACTCTTGAGAAAAAACGTCGATCAACTCGATTACAAGTTCATAACGTATACCTGCAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGGTACACATCTTCTTGTAGCTTCAAGTCATTCTTTGGCTTTGAATAAAGTTCCCTATTACTTGTTGTAACTAGCTTTATCCAAATTCACTGTACCAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCGGACTTCGTCAAGCCAGGTAAATACATGTTGCCCTTGATAATATGTTCTTGCCTCTGTTCAGTTCATTAAACATTTGCATATATTATGAAAGATTGGTGGAGAGAGATTCATATATTACCATCCAAGCTTCTGGGCTGTCCTTGAGAAGTTGCTGCTTTCAATTCCTACTTCATTTTCACTTTGCCATCTCATTAGGGGTGAGACTGTTTTCACAACACCTATAAGTTCTTCTACAGATGAACGTCTGAACGATGTCGTCGGTAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCGACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCACAAGCTCTAAGTAAAATCTTCAGGCTCACTTTTTCTAGTACCAATAAAATGTATACAAGTATATCTGTTGATAAGCAAACAATTTCGCTCCTTTGATTCACAGCACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTTTGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGTAATCTGTCACTGCAATTGAACATATCACAAATGGTGCATCAATAACTTTAACGACATCTCTACATTATTGTAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGATCCACAAAATGGATTTGTAACCATTGATAACTACAAAGCGGTTAGGCTCCCGCCATTCTTTTTGCTTCGACGTGTACTTTGTAATCTTTACTGTTGATGCATAAATATTATGAGTTGACCTTATGCAGGCTCTAGTGAGAAACGTAACTGATGCAATGAGAGAATCCAATGTTGCTGACATTCTGAAGATGGTGAGATCACTTTTACTTCTTGGCCTTCAATTTAGACACCTTTTGTGAGATCCCCTATCGATGGGAGAGGACGAGAACGAAATAACCTTTATAAGAGTGTGGAAACCTCTCCTTAGCACGTTTTAAAGCCTTGAGGAGGAAGCTCGAAAGGGAAAGCCCAAAGAGGACAATATCAGCTAGCAATGGGCTTGATTTCCTACAAATGATATCAGAGTCAAACACCAAGCGATGTACCAACGAAGAGGTTGAACTCCGAAGGGGGTGGACAAAAAAAGGCGGTGTGCCAACAAGGACGCTAGGCCCTTAGGGGGTGGATTTGGGGGTCCTACATCGATTGGAGAAGGGAATGAACGCCAGTAAAGACGTTGGGCCCTGAAAGGCAGTAAACACGTTGGGCCCTGAAGGGAGGTGGATTGTTAGATCCACGTCGATTGGGGAGGAGAACGAAATACCCTTTATAAGGGTGTGGAAACCTCTCCCTATAAAGGGAAGTCCAAAGATGACAATACCTGCTAGCAGTGGGCTTGGTTGTTACACCTTTTATGCATATACCAAAATCAAGTTACATTCTCTGCAACTTTGGCATAATGCTCTCCGGCCGTGGTTGATATTCTTTTTCACTAACATTTCCAACTTGTATTTAGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTAGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGATGGAAATCGAGTCATTTCAGTACATGAGTTAGTTCAGGTAAGAAACAATTCGACGCTTGGAATTACTACATTTTACACAACTAACAACAGTGAATTGAAGTGCAAGTTTAAAATGTGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAGATTTCTACATGGTGTTACAATCCGCTACTCTAATACAAGACATTAACACACAGAAGAAGCAGATAGTTCAATAGCTGTACACTTGATTCATTTTTCCTCCTTATTGTATTATCAATTTGATTCCTAGCAGCTTCAAAACACTGAATAAAAGAGGGCCATGCGATTGAGAAACATAAAAAGAAACGAGGATGTACCATGTATTGCATTCTTTCCCATTTCACCGCCCAGTTCTTTATAATCCTTCCTACATGTAAAAACAAGGCAGAGATCAGAATACAGATTTTACTG

mRNA sequence

ATGGGACACCACTGTAGTAAAAGCGTTTCTGATATCAGTAACCATGCTGTAATTTCCGGTGGCAGCCATCCAACCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTGGAATAGCGCCGTCGCCAGCCAGAACTCCGAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCACCAGCAAAGCCCATCATGGCCATTCTCCGGCGGAAATCACGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTAGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGATGAACTTTGGAGCGAAGTTCGAGCTCGGGAAGGAGGTGGGGAGGGGCCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAAGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAATCTAAGATGACATCGACTGTTGGAATCGAAGATGTTCGCCGGGAGGTAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGTGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGCTTTATCCAAATTCACTGTACCAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCGGACTTCGTCAAGCCAGAGAGATTCATATATTACCATCCAAGCTTCTGGGCTGTCCTTGAGAAGTTGCTGCTTTCAATTCCTACTTCATTTTCACTTTGCCATCTCATTAGGGGTGAGACTGTTTTCACAACACCTATAAGTTCTTCTACAGATGAACGTCTGAACGATGTCGTCGGTAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCGACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCACAAGCTCTAACACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTTTGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGATCCACAAAATGGATTTGTAACCATTGATAACTACAAAGCGGCTCTAGTGAGAAACGTAACTGATGCAATGAGAGAATCCAATGTTGCTGACATTCTGAAGATGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTAGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGATGGAAATCGAGTCATTTCAGTACATGAGTTAGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAGATTTCTACATGGTGTTACAATCCGCTACTCTAATACAAGACATTAACACACAGAAGAAGCAGATAGTTCATTTTCCTCCTTATTGTATTATCAATTTGATTCCTAGCAGCTTCAAAACACTGAATAAAAGAGGGCCATGCGATTGAGAAACATAAAAAGAAACGAGGATGTACCATGTATTGCATTCTTTCCCATTTCACCGCCCAGTTCTTTATAATCCTTCCTACATGTAAAAACAAGGCAGAGATCAGAATACAGATTTTACTG

Coding sequence (CDS)

ATGGGACACCACTGTAGTAAAAGCGTTTCTGATATCAGTAACCATGCTGTAATTTCCGGTGGCAGCCATCCAACCGAAGATACCCGGCACTCCAACTCTGTCTCTGCAACAGCGAGCCCATTTTCTAGTCCTCTGCCTTCTGGAATAGCGCCGTCGCCAGCCAGAACTCCGAGGAGGAAATTCCGATGGCCTCTACCGCCTCCTTCACCAGCAAAGCCCATCATGGCCATTCTCCGGCGGAAATCACGCAAGGCTTCGATACCAGAGGAAAACCAAATTGGGGAAGAGGGGTTAGGAGAGGTTCAGTTGGATAAAAGCTTTGGGTACTCGATGAACTTTGGAGCGAAGTTCGAGCTCGGGAAGGAGGTGGGGAGGGGCCATTTTGGACATACTTTCTGGGCTAAGGGTAAAAAAGGCGACCTCAAAGGAAAGCCTGTGGCCGTCAAAATCATCTCTAAATCTAAGATGACATCGACTGTTGGAATCGAAGATGTTCGCCGGGAGGTAAAGATACTGAAAGCATTGTCCGGGCACAAGAATCTGGTCCATTTCCACGGTGCATTTGAAGATGCCAATAACATTTACATAGTTATGGAATTGTGTGAAGGCGGAGAACTGCTAGAAAGAATAGTGTCAAGAGGAGGAAGATATCAAGAACAAGAATCGAAAACGATCATTGTGCAGATACTCTGTGTTGTTGCATTTTGTCATCTACAAGGGATAGTGCACCGTGACTTGAAGCCAGAGCTTTATCCAAATTCACTGTACCAGAATTTCCTTTTTATGAAGAAAGATGAGAACACAGGGCTCAAACTCATTGATTTTGGTCTCTCGGACTTCGTCAAGCCAGAGAGATTCATATATTACCATCCAAGCTTCTGGGCTGTCCTTGAGAAGTTGCTGCTTTCAATTCCTACTTCATTTTCACTTTGCCATCTCATTAGGGGTGAGACTGTTTTCACAACACCTATAAGTTCTTCTACAGATGAACGTCTGAACGATGTCGTCGGTAGTGCATACTATGTTGCTCCTGAAGTACTCTACAGATCCTACAGTTTTGAAGCAGACATGTGGAGTATTGGTGTCATAGCATATATACTGTTGTGTGGGAGCAGACCTTTCTGGGCAAGAACTGAATCAGGAATCTTCCGCTCTGTGCTAAGAGCCGATCCTAACTTCGATGATTCCCCTTGGCCGACCATCTCTCCAGAAGCTAAAGATTTTGTGAAACGCCTTCTGAATAAAGACCATAGAAAAAGAATGACAGCAGCACAAGCTCTAACACACCCGTGGTTACGAGATGAAGATCTTGCCGTGCCTTTGGATAACTTCATATACAAGTCAGTTAAGGCATATCTTTGTGCAACTCCTTTCAAGCGTGCAGCATTGAAGGCACTTGCAAAAGCTTTGACAGAAGATGAACTTTTCTACCTCAGGACCCAATTCAAGCTTTTGGATCCACAAAATGGATTTGTAACCATTGATAACTACAAAGCGGCTCTAGTGAGAAACGTAACTGATGCAATGAGAGAATCCAATGTTGCTGACATTCTGAAGATGATGGAACCTCTCGCTAACAAAAGAATGGGCTTTGAGGAGTTCTGCGCAGCTGCAATCAGTGTATATCAGCTGGAGGCTGTAGCAGGATGGGAAAGCATCGCCACCCGAGCTTTCGAGTATTTCGAGCAGGATGGAAATCGAGTCATTTCAGTACATGAGTTAGTTCAGGAAATGAATCTGGGTCCTATAGCTCATTCTTTTCTCCAGAATTGGACAAGAAGTTCTGATGGGAAACTCAGTTTTTTCGGATATACAAGATTTCTACATGGTGTTACAATCCGCTACTCTAATACAAGACATTAA

Protein sequence

MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH
Homology
BLAST of Carg26781 vs. NCBI nr
Match: KAG7016799.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1246.9 bits (3225), Expect = 0.0e+00
Identity = 620/620 (100.00%), Postives = 620/620 (100.00%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
           MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60

Query: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
           FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG
Sbjct: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120

Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
           KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180

Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
           LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240

Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
           IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL
Sbjct: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300

Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
           LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360

Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
           GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420

Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
           MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480

Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
           QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540

Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
           VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600

Query: 601 FFGYTRFLHGVTIRYSNTRH 621
           FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 620

BLAST of Carg26781 vs. NCBI nr
Match: XP_022922250.1 (CDPK-related kinase 4 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 562/620 (90.65%), Postives = 564/620 (90.97%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
           MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60

Query: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
           FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120

Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
           +EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180

Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
           LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240

Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
           IVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP                 
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300

Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
                                       DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360

Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
           GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420

Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
           MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480

Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
           QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540

Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
           VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 567

Query: 601 FFGYTRFLHGVTIRYSNTRH 621
           FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 567

BLAST of Carg26781 vs. NCBI nr
Match: XP_023549645.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1078.2 bits (2787), Expect = 0.0e+00
Identity = 554/625 (88.64%), Postives = 560/625 (89.60%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
           MGHHCSKSVSD+SNHAVISGGS P+     EDTRHSNSVSATASPFSSPLPSGIAPSPAR
Sbjct: 1   MGHHCSKSVSDVSNHAVISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSGIAPSPAR 60

Query: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
           TPRRKFRWPLPPPSPAKPIMAILRRKSRKASI EENQI EEGLGEVQLDKSFGYS NFGA
Sbjct: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIQEENQIREEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
           KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTST+ IEDVRREVKILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTIAIEDVRREVKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
           CHLQGIVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP            
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300

Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
                                            DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360

Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
           DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420

Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
           DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480

Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
           FYLRTQFKLL+PQNGFVTIDNYK ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKTALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540

Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
           AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 572

Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
           DGKLSFFGYTRFLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 572

BLAST of Carg26781 vs. NCBI nr
Match: XP_022974330.1 (CDPK-related kinase 6-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1069.3 bits (2764), Expect = 1.2e-308
Identity = 551/625 (88.16%), Postives = 560/625 (89.60%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
           T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61  T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
           KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
           CHLQGIVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP            
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300

Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
                                            DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360

Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
           DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420

Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
           DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480

Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
           FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540

Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
           AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 571

Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
           DGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTKFLHGVTIRYSNTRH 571

BLAST of Carg26781 vs. NCBI nr
Match: KAG6579299.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1016.9 bits (2628), Expect = 7.3e-293
Identity = 523/579 (90.33%), Postives = 523/579 (90.33%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
           MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60

Query: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
           FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120

Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
           KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180

Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
           LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240

Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
           IVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP                 
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300

Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
                                       DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360

Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
           GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420

Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
           MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480

Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
           QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 526

Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
           VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQ  N
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQVRN 526

BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match: Q9FIM9 (CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1)

HSP 1 Score: 708.0 bits (1826), Expect = 9.5e-203
Identity = 369/646 (57.12%), Postives = 467/646 (72.29%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISG----GSHPTEDTRHSNSV------------SATASPFSSP 60
           MGH  S+++S + +  + +G     + P+++ RH++              S   SPF SP
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60

Query: 61  LPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQI----- 120
           LP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+       R   IPE+++      
Sbjct: 61  LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120

Query: 121 GEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
           G+ G GE +LDK+FG+  NF  K+ELGKEVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180

Query: 181 SKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
           +KMTST+ IEDVRREVK+LKALSGH+++V F+  +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240

Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLI 300
           GGRY E ++K I+VQIL   AF HLQG+VHRDLKPE        NFLF  ++E+  LK+I
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSRNEDAILKVI 300

Query: 301 DFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLN 360
           DFGLSDF++                                              D+RLN
Sbjct: 301 DFGLSDFIR---------------------------------------------YDQRLN 360

Query: 361 DVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNF 420
           DVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNF
Sbjct: 361 DVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNF 420

Query: 421 DDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL 480
           +D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  +YK VK+Y+
Sbjct: 421 EDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVYKLVKSYI 480

Query: 481 CATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESN 540
            A+PF+R+ALKAL+KA+ ++EL +L+ QF LLDP++G ++++ +  AL R  TDAM ES 
Sbjct: 481 RASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYATDAMMESR 540

Query: 541 VADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHE 600
           + DIL  M+PLA K++ FEEFCAAA+SVYQLEA+  WE IAT AFE+FE +GNR+ISV E
Sbjct: 541 LPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGNRIISVQE 592

Query: 601 LVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
           L  EM++GP A+  L++W RSSDGKLSF GY +FLHGVT+R S++R
Sbjct: 601 LAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match: Q9SG12 (CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 1.8e-201
Identity = 369/645 (57.21%), Postives = 461/645 (71.47%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGS----HPTEDTRHSNSVSAT--------------ASPFS 60
           MGH  S+++S + +   I   +    H +    H  S S++               SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQIGEE 120
           SPLP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+       R   IPE+++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --GLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKS 180
             G+GE +LDK+FG++ NF  K+ELG+EVGRGHFGHT WAK KKG +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
           KMTS + IEDVRREVK+LKALSGH ++V F+  FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLID 300
           GRY E E+K I+VQIL   AF HLQG+VHRDLKPE        NFLF  K+E+  LK+ID
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSKNEDAVLKVID 300

Query: 301 FGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLND 360
           FGLSD+ +                                              D+RLND
Sbjct: 301 FGLSDYAR---------------------------------------------FDQRLND 360

Query: 361 VVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFD 420
           VVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNFD
Sbjct: 361 VVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNFD 420

Query: 421 DSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLC 480
           D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK VK+Y+ 
Sbjct: 421 DLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYKLVKSYIR 480

Query: 481 ATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNV 540
           A+PF+RAALK+L+KA+ E+EL +L+ QF LL+P++G + + N+  AL R  TDAM ES +
Sbjct: 481 ASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATDAMIESRL 540

Query: 541 ADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHEL 600
            DIL MM+PLA+K++ FEEFCAA++SVYQLEA+  WE IAT AFE+FE +G+R ISV EL
Sbjct: 541 PDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSRAISVQEL 591

Query: 601 VQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
            +EM+LGP A+  L++W RS DGKL+F GY +FLHGVT+R S++R
Sbjct: 601 AEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match: Q9ZUZ2 (CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1)

HSP 1 Score: 700.7 bits (1807), Expect = 1.5e-200
Identity = 367/600 (61.17%), Postives = 445/600 (74.17%), Query Frame = 0

Query: 31  SNSVSATASPF----SSPLPSGIAPSPAR--TPRRKFRWPLPPPSPAKPIMAILRR---- 90
           S++ S   SPF    +SPLPSG++PSPAR  TPRR FR P PPPSPAK I A L +    
Sbjct: 51  SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110

Query: 91  KSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGD 150
           K ++  IPEE   G E   E  LDKSFGY  NFGAK+ELGKEVGRGHFGHT   +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170

Query: 151 LKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMEL 210
           +K  P+AVKIISK+KMT+ + IEDVRREVK+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230

Query: 211 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFL 270
           C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPE        NFL
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPE--------NFL 290

Query: 271 FMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVF 330
           F    E++ LKLIDFGLSDF++P                                     
Sbjct: 291 FTSSREDSDLKLIDFGLSDFIRP------------------------------------- 350

Query: 331 TTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTES 390
                   DERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRPFWARTES
Sbjct: 351 --------DERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTES 410

Query: 391 GIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVP 450
           GIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLRD+   +P
Sbjct: 411 GIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLRDDSRVIP 470

Query: 451 LDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKA 510
           LD  IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V+++N+K 
Sbjct: 471 LDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSVSLENFKT 530

Query: 511 ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFE 570
           AL++N TDAMRES V +IL  ME LA ++M FEEFCAAAIS++QLEAV  WE IAT  F+
Sbjct: 531 ALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEEIATAGFQ 590

Query: 571 YFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
           +FE +GNRVI++ EL +E+N+G  A+  L++W RSSDGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 591 HFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVTLRAAHAR 593

BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match: Q9SCS2 (CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 1.4e-177
Identity = 329/596 (55.20%), Postives = 421/596 (70.64%), Query Frame = 0

Query: 40  PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
           PF SP P+         A SPA      TP+R F+ P PPPSPAK I A+L R     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
             ++IPE ++    G+G   LDKSFG+S +F +K+ELG EVGRGHFG+T  AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
           G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
           GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE        NFLF 
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296

Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
            K++ + LK IDFGLSD+V+P                                       
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRP--------------------------------------- 356

Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
                 DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 ------DERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416

Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
           FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D  D  VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476

Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
           D  ++K ++AYL ++  ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536

Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
           L++  TDAM++S + + L  +  L  +RM FEEFCAAA+SV+QLEA+  WE  A  A+E 
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596

Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
           FE++GNR I + EL  E+ LGP    H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 596

BLAST of Carg26781 vs. ExPASy Swiss-Prot
Match: P53681 (CDPK-related protein kinase OS=Daucus carota OX=4039 GN=CRK PE=2 SV=1)

HSP 1 Score: 620.2 bits (1598), Expect = 2.6e-176
Identity = 340/639 (53.21%), Postives = 428/639 (66.98%), Query Frame = 0

Query: 3   HHCSKSVSDIS-----NHAVISGGSHPTEDTRHSNSVSATASPFSSPLP----------- 62
           HH S  V+D S     N       + P +D        +   PF SP P           
Sbjct: 17  HHTSIPVNDTSLPPQDNSIPPKDIAIPAQDNNKPPGKKSPFLPFYSPSPAHFLFSKKSPA 76

Query: 63  --SGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRR-----KSRKASIPEENQIGEEGLG 122
             S  A S   TP+R F  P PPPSPAK I A   R     K  +A+IPE N++     G
Sbjct: 77  VGSPAAGSSNSTPKRLF--PFPPPSPAKHIKAAWARRHGSVKPNEAAIPENNEVD----G 136

Query: 123 EVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTST 182
              LDKSFG+S  FG+KFE+G+EVGRGHFG+T  AK KKG+ KG+ VAVK+I K+KMT+ 
Sbjct: 137 GAGLDKSFGFSKKFGSKFEVGEEVGRGHFGYTCRAKFKKGEFKGQDVAVKVIPKAKMTTA 196

Query: 183 VGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQE 242
           + IEDVRREVKIL+AL+GH NLV F+ AFED  N+Y+VMELCEGGELL+RI+SRGG+Y E
Sbjct: 197 IAIEDVRREVKILRALTGHNNLVQFYDAFEDHTNVYVVMELCEGGELLDRILSRGGKYTE 256

Query: 243 QESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSD 302
            ++K +++QIL VVAFCHLQG+VHRDLKPE        NFLF  KDE++ LK IDFGLSD
Sbjct: 257 DDAKAVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFKSKDEDSQLKAIDFGLSD 316

Query: 303 FVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSA 362
           +VKP                                             DERLND+VGSA
Sbjct: 317 YVKP---------------------------------------------DERLNDIVGSA 376

Query: 363 YYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWP 422
           YYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPFWARTESGIFR+VL+A+ +FD+ PWP
Sbjct: 377 YYVAPEVLHRSYSTEADVWSIGVISYILLCGSRPFWARTESGIFRAVLKANLSFDEPPWP 436

Query: 423 TISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPLDNFIYKSVKAYLCATPF 482
           ++S EAKDFVKRLLNKD RKRMTAAQAL H W+++  D+  PLD  ++K +K Y+ ++P 
Sbjct: 437 SVSSEAKDFVKRLLNKDPRKRMTAAQALCHSWIKNSNDIKFPLDILVFKLMKVYMRSSPL 496

Query: 483 KRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAALVRNVTDAMRESNVADI 542
           ++AAL+AL+K LT DELFYL+ QF LL+P +NG ++++N K AL+RN TDAM++S V D+
Sbjct: 497 RKAALRALSKTLTVDELFYLKEQFVLLEPTKNGTISLENIKQALMRNSTDAMKDSRVLDL 556

Query: 543 LKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQE 602
           L  +  L  +RM FEEFCAAA+SV+QLEA+  WE  A  A++ FE+DGNR I + EL  E
Sbjct: 557 LVSLNALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYDLFEKDGNRAIMIEELASE 596

Query: 603 MNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
           + LGP    H+ L +W R +DGKLSF GY + LHGV+ R
Sbjct: 617 LGLGPSIPVHAVLHDWIRHTDGKLSFLGYVKLLHGVSTR 596

BLAST of Carg26781 vs. ExPASy TrEMBL
Match: A0A6J1E874 (CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)

HSP 1 Score: 1098.2 bits (2839), Expect = 0.0e+00
Identity = 562/620 (90.65%), Postives = 564/620 (90.97%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
           MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60

Query: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
           FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120

Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
           +EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180

Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
           LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240

Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
           IVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP                 
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300

Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
                                       DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360

Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
           GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420

Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
           MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480

Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
           QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540

Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 600
           VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLS 567

Query: 601 FFGYTRFLHGVTIRYSNTRH 621
           FFGYTRFLHGVTIRYSNTRH
Sbjct: 601 FFGYTRFLHGVTIRYSNTRH 567

BLAST of Carg26781 vs. ExPASy TrEMBL
Match: A0A6J1IDP0 (CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)

HSP 1 Score: 1069.3 bits (2764), Expect = 6.0e-309
Identity = 551/625 (88.16%), Postives = 560/625 (89.60%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
           T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61  T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
           KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
           CHLQGIVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP            
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300

Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
                                            DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360

Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
           DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420

Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
           DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480

Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
           FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540

Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 600
           AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQEMNLGPIAHSFLQNWTRSS
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPIAHSFLQNWTRSS 571

Query: 601 DGKLSFFGYTRFLHGVTIRYSNTRH 621
           DGKLSFFGYT+FLHGVTIRYSNTRH
Sbjct: 601 DGKLSFFGYTKFLHGVTIRYSNTRH 571

BLAST of Carg26781 vs. ExPASy TrEMBL
Match: A0A6J1E2V0 (CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)

HSP 1 Score: 1006.5 bits (2601), Expect = 4.8e-290
Identity = 519/579 (89.64%), Postives = 521/579 (89.98%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTPRRK 60
           MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPART RRK
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHPTEDTRHSNSVSATASPFSSPLPSGIAPSPARTTRRK 60

Query: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELG 120
           FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGAKFELG
Sbjct: 61  FRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGAKFELG 120

Query: 121 KEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180
           +EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN
Sbjct: 121 REVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKN 180

Query: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240
           LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG
Sbjct: 181 LVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQG 240

Query: 241 IVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKL 300
           IVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP                 
Sbjct: 241 IVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP----------------- 300

Query: 301 LLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360
                                       DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI
Sbjct: 301 ----------------------------DERLNDVVGSAYYVAPEVLYRSYSFEADMWSI 360

Query: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420
           GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR
Sbjct: 361 GVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKR 420

Query: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRT 480
           MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDELFYLRT
Sbjct: 421 MTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRT 480

Query: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 540
           QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS
Sbjct: 481 QFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAIS 526

Query: 541 VYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
           VYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQ  N
Sbjct: 541 VYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 526

BLAST of Carg26781 vs. ExPASy TrEMBL
Match: A0A6J1IHA7 (CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)

HSP 1 Score: 978.8 bits (2529), Expect = 1.1e-281
Identity = 509/584 (87.16%), Postives = 517/584 (88.53%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHPT-----EDTRHSNSVSATASPFSSPLPSGIAPSPAR 60
           MGHHCSKSVSD+S+HA ISGGS P+     EDTRHSNSVSATASPFSSPLPS IAPSPAR
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSSSPIPEDTRHSNSVSATASPFSSPLPSRIAPSPAR 60

Query: 61  TPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGA 120
           T RRKFRWPLPPPSPAKPIMAILR+KSRKASIPEENQIGEEGLGEVQLDKSFGYS NFGA
Sbjct: 61  T-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQLDKSFGYSTNFGA 120

Query: 121 KFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGIEDVRREVKILKAL 180
           KFELG+EVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK+KMTSTVGIEDVRRE KILKAL
Sbjct: 121 KFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGIEDVRREEKILKAL 180

Query: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240
           SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF
Sbjct: 181 SGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQESKTIIVQILCVVAF 240

Query: 241 CHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWA 300
           CHLQGIVHRDLKPE        NFLFMKKDENTGLKLIDFGLSDFVKP            
Sbjct: 241 CHLQGIVHRDLKPE--------NFLFMKKDENTGLKLIDFGLSDFVKP------------ 300

Query: 301 VLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEA 360
                                            DERLNDVVGSAYYVAPEVLYRSYSFEA
Sbjct: 301 ---------------------------------DERLNDVVGSAYYVAPEVLYRSYSFEA 360

Query: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420
           DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK
Sbjct: 361 DMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNK 420

Query: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAALKALAKALTEDEL 480
           DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL ATPFKRAALKALAKALTEDEL
Sbjct: 421 DHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDEL 480

Query: 481 FYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 540
           FYLRTQFKLL+PQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC
Sbjct: 481 FYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFC 530

Query: 541 AAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMN 580
           AAAISVYQLEAVAGWESIATRAFEYFEQ+GNRVISVHELVQ  N
Sbjct: 541 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQVRN 530

BLAST of Carg26781 vs. ExPASy TrEMBL
Match: A0A6J1K6B2 (CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1)

HSP 1 Score: 974.5 bits (2518), Expect = 2.0e-280
Identity = 505/638 (79.15%), Postives = 530/638 (83.07%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGSHP------------------TEDTRHSNSVSATASPFS 60
           MGH CSKSVSD+++   ISGGSH                    E+ R S SVSAT SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60

Query: 61  SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQ 120
           SPLP+GIAPSPARTP RKFRWPLPPPSPAKPIMAILRRKSRKASIPEEN+IGEEG+GE+Q
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGI 180
           LDKSFGYS NFGAKFELGKE+GRGHFGHTFWA+GKKGDLKGKPVAVKIISKSKMTS VGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQES 240
           EDVRREVKILKALSGHKNLV FH AFEDANNIY+VMELCEGGELLERIVSRGGRY EQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLIDFGLSDFVK 300
           KTI+VQIL VVAF HLQG+VHRDLKPE        NFLF+KKDENTGLK+IDFGLSDFVK
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPE--------NFLFVKKDENTGLKVIDFGLSDFVK 300

Query: 301 PERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLNDVVGSAYYV 360
           P                                             DE LNDVVGSAYYV
Sbjct: 301 P---------------------------------------------DEHLNDVVGSAYYV 360

Query: 361 APEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDDSPWPTIS 420
           APEVLYRSYS EAD+WSIGVIAYILLCG RPFWARTESGIFRSVLRADPNFDDSPWPTIS
Sbjct: 361 APEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTIS 420

Query: 421 PEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLCATPFKRAA 480
            EAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYL  TPFKRAA
Sbjct: 421 AEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRVTPFKRAA 480

Query: 481 LKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVADILKMME 540
           LKALAKALTEDELFYLR QFKLL+PQNGFVT+DN+K ALVRN TDAMRESNV DILKM E
Sbjct: 481 LKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVLDILKMTE 540

Query: 541 PLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHELVQEMNLGP 600
            +A +RM FEEFCAAAISVYQLEAVAGWE IATRAFEYFEQDGNRVISVHELVQEMNL P
Sbjct: 541 SIAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELVQEMNLSP 585

Query: 601 IAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 621
            AHSFLQNWTRSSDGKLSFFGYTRFLHGVT+R SNTRH
Sbjct: 601 AAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Carg26781 vs. TAIR 10
Match: AT5G24430.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 708.0 bits (1826), Expect = 6.7e-204
Identity = 369/646 (57.12%), Postives = 467/646 (72.29%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISG----GSHPTEDTRHSNSV------------SATASPFSSP 60
           MGH  S+++S + +  + +G     + P+++ RH++              S   SPF SP
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVNSYNISPFQSP 60

Query: 61  LPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQI----- 120
           LP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+       R   IPE+++      
Sbjct: 61  LPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSEDVVDHG 120

Query: 121 GEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISK 180
           G+ G GE +LDK+FG+  NF  K+ELGKEVGRGHFGHT WAK KKG +K + VAVKIISK
Sbjct: 121 GDSGGGE-RLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAVKIISK 180

Query: 181 SKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSR 240
           +KMTST+ IEDVRREVK+LKALSGH+++V F+  +EDA+N+++VMELCEGGELL+RI++R
Sbjct: 181 AKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLDRILAR 240

Query: 241 GGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLI 300
           GGRY E ++K I+VQIL   AF HLQG+VHRDLKPE        NFLF  ++E+  LK+I
Sbjct: 241 GGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSRNEDAILKVI 300

Query: 301 DFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLN 360
           DFGLSDF++                                              D+RLN
Sbjct: 301 DFGLSDFIR---------------------------------------------YDQRLN 360

Query: 361 DVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNF 420
           DVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNF
Sbjct: 361 DVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNF 420

Query: 421 DDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYL 480
           +D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  +YK VK+Y+
Sbjct: 421 EDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSVYKLVKSYI 480

Query: 481 CATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESN 540
            A+PF+R+ALKAL+KA+ ++EL +L+ QF LLDP++G ++++ +  AL R  TDAM ES 
Sbjct: 481 RASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMALTRYATDAMMESR 540

Query: 541 VADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHE 600
           + DIL  M+PLA K++ FEEFCAAA+SVYQLEA+  WE IAT AFE+FE +GNR+ISV E
Sbjct: 541 LPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHFEHEGNRIISVQE 592

Query: 601 LVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
           L  EM++GP A+  L++W RSSDGKLSF GY +FLHGVT+R S++R
Sbjct: 601 LAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of Carg26781 vs. TAIR 10
Match: AT3G49370.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 703.7 bits (1815), Expect = 1.3e-202
Identity = 369/645 (57.21%), Postives = 461/645 (71.47%), Query Frame = 0

Query: 1   MGHHCSKSVSDISNHAVISGGS----HPTEDTRHSNSVSAT--------------ASPFS 60
           MGH  S+++S + +   I   +    H +    H  S S++               SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPSGIAPSPARTPRRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEENQIGEE 120
           SPLP+G+APSPARTP RKF+WP PPPSPAKPIMA LRR+       R   IPE+++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --GLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKS 180
             G+GE +LDK+FG++ NF  K+ELG+EVGRGHFGHT WAK KKG +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRG 240
           KMTS + IEDVRREVK+LKALSGH ++V F+  FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFMKKDENTGLKLID 300
           GRY E E+K I+VQIL   AF HLQG+VHRDLKPE        NFLF  K+E+  LK+ID
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPE--------NFLFTSKNEDAVLKVID 300

Query: 301 FGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTTPISSSTDERLND 360
           FGLSD+ +                                              D+RLND
Sbjct: 301 FGLSDYAR---------------------------------------------FDQRLND 360

Query: 361 VVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFD 420
           VVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCGSRPF+ RTES IFR VLRA+PNFD
Sbjct: 361 VVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVLRANPNFD 420

Query: 421 DSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLC 480
           D PWP+ISP AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK VK+Y+ 
Sbjct: 421 DLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYKLVKSYIR 480

Query: 481 ATPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNV 540
           A+PF+RAALK+L+KA+ E+EL +L+ QF LL+P++G + + N+  AL R  TDAM ES +
Sbjct: 481 ASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATDAMIESRL 540

Query: 541 ADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQDGNRVISVHEL 600
            DIL MM+PLA+K++ FEEFCAA++SVYQLEA+  WE IAT AFE+FE +G+R ISV EL
Sbjct: 541 PDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSRAISVQEL 591

Query: 601 VQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
            +EM+LGP A+  L++W RS DGKL+F GY +FLHGVT+R S++R
Sbjct: 601 AEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of Carg26781 vs. TAIR 10
Match: AT2G46700.1 (CDPK-related kinase 3 )

HSP 1 Score: 700.7 bits (1807), Expect = 1.1e-201
Identity = 367/600 (61.17%), Postives = 445/600 (74.17%), Query Frame = 0

Query: 31  SNSVSATASPF----SSPLPSGIAPSPAR--TPRRKFRWPLPPPSPAKPIMAILRR---- 90
           S++ S   SPF    +SPLPSG++PSPAR  TPRR FR P PPPSPAK I A L +    
Sbjct: 51  SSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPPPSPAKHIKASLIKRLGV 110

Query: 91  KSRKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGD 150
           K ++  IPEE   G E   E  LDKSFGY  NFGAK+ELGKEVGRGHFGHT   +GKKGD
Sbjct: 111 KPKEGPIPEER--GTE--PEQSLDKSFGYGKNFGAKYELGKEVGRGHFGHTCSGRGKKGD 170

Query: 151 LKGKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMEL 210
           +K  P+AVKIISK+KMT+ + IEDVRREVK+LK+LSGHK L+ ++ A EDANN+YIVMEL
Sbjct: 171 IKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIKYYDACEDANNVYIVMEL 230

Query: 211 CEGGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFL 270
           C+GGELL+RI++RGG+Y E ++K I+VQIL VV+FCHLQG+VHRDLKPE        NFL
Sbjct: 231 CDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVHRDLKPE--------NFL 290

Query: 271 FMKKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVF 330
           F    E++ LKLIDFGLSDF++P                                     
Sbjct: 291 FTSSREDSDLKLIDFGLSDFIRP------------------------------------- 350

Query: 331 TTPISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTES 390
                   DERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI YILLCGSRPFWARTES
Sbjct: 351 --------DERLNDIVGSAYYVAPEVLHRSYSLEADIWSIGVITYILLCGSRPFWARTES 410

Query: 391 GIFRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVP 450
           GIFR+VLR +PN+DD PWP+ S E KDFVKRLLNKD+RKRM+A QALTHPWLRD+   +P
Sbjct: 411 GIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKDYRKRMSAVQALTHPWLRDDSRVIP 470

Query: 451 LDNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKA 510
           LD  IYK VKAYL ATP +RAALKALAKALTE+EL YLR QF LL P ++G V+++N+K 
Sbjct: 471 LDILIYKLVKAYLHATPLRRAALKALAKALTENELVYLRAQFMLLGPNKDGSVSLENFKT 530

Query: 511 ALVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFE 570
           AL++N TDAMRES V +IL  ME LA ++M FEEFCAAAIS++QLEAV  WE IAT  F+
Sbjct: 531 ALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFCAAAISIHQLEAVDAWEEIATAGFQ 590

Query: 571 YFEQDGNRVISVHELVQEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTR 620
           +FE +GNRVI++ EL +E+N+G  A+  L++W RSSDGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 591 HFETEGNRVITIEELARELNVGASAYGHLRDWVRSSDGKLSYLGFTKFLHGVTLRAAHAR 593

BLAST of Carg26781 vs. TAIR 10
Match: AT3G50530.2 (CDPK-related kinase )

HSP 1 Score: 636.0 bits (1639), Expect = 3.3e-182
Identity = 334/596 (56.04%), Postives = 434/596 (72.82%), Query Frame = 0

Query: 40  PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
           PF SP P+         A SPA      TP+R F+ P PPPSPAK I A+L R     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
             ++IPE ++    G+G   LDKSFG+S +F +K+ELG EVGRGHFG+T  AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
           G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
           GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE        NFLF 
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296

Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
            K++ + LK IDFGLSD+V+P + +      +A+ +    ++ TS  L  L         
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRPGKAL----RLYAICKLRFQNLETSICLYAL--------- 356

Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
              +  DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 -TIAFADERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416

Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
           FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D  D  VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476

Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
           D  ++K ++AYL ++  ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536

Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
           L++  TDAM++S + + L  +  L  +RM FEEFCAAA+SV+QLEA+  WE  A  A+E 
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596

Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
           FE++GNR I + EL  E+ LGP    H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 627

BLAST of Carg26781 vs. TAIR 10
Match: AT3G50530.1 (CDPK-related kinase )

HSP 1 Score: 624.4 bits (1609), Expect = 9.8e-179
Identity = 329/596 (55.20%), Postives = 421/596 (70.64%), Query Frame = 0

Query: 40  PFSSPLPSGI-------APSPA-----RTPRRKFRWPLPPPSPAKPIMAILRR-----KS 99
           PF SP P+         A SPA      TP+R F+ P PPPSPAK I A+L R     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 100 RKASIPEENQIGEEGLGEVQLDKSFGYSMNFGAKFELGKEVGRGHFGHTFWAKGKKGDLK 159
             ++IPE ++    G+G   LDKSFG+S +F +K+ELG EVGRGHFG+T  AK KKGD K
Sbjct: 117 NSSAIPEGSEAEGGGVG---LDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 160 GKPVAVKIISKSKMTSTVGIEDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCE 219
           G+ VAVK+I K+KMT+ + IEDVRREVKIL+ALSGH NL HF+ A+ED +N+YIVMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 220 GGELLERIVSRGGRYQEQESKTIIVQILCVVAFCHLQGIVHRDLKPELYPNSLYQNFLFM 279
           GGELL+RI+SRGG+Y E+++KT+++QIL VVAFCHLQG+VHRDLKPE        NFLF 
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPE--------NFLFT 296

Query: 280 KKDENTGLKLIDFGLSDFVKPERFIYYHPSFWAVLEKLLLSIPTSFSLCHLIRGETVFTT 339
            K++ + LK IDFGLSD+V+P                                       
Sbjct: 297 SKEDTSQLKAIDFGLSDYVRP--------------------------------------- 356

Query: 340 PISSSTDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGI 399
                 DERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCGSRPFWARTESGI
Sbjct: 357 ------DERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFWARTESGI 416

Query: 400 FRSVLRADPNFDDSPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRD-EDLAVPL 459
           FR+VL+ADP+FDD PWP +S EA+DFVKRLLNKD RKR+TAAQAL+HPW++D  D  VP+
Sbjct: 417 FRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDSNDAKVPM 476

Query: 460 DNFIYKSVKAYLCATPFKRAALKALAKALTEDELFYLRTQFKLLDP-QNGFVTIDNYKAA 519
           D  ++K ++AYL ++  ++AAL+AL+K LT DELFYLR QF LL+P +NG ++++N K+A
Sbjct: 477 DILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTISLENIKSA 536

Query: 520 LVRNVTDAMRESNVADILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEY 579
           L++  TDAM++S + + L  +  L  +RM FEEFCAAA+SV+QLEA+  WE  A  A+E 
Sbjct: 537 LMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQHARCAYEL 596

Query: 580 FEQDGNRVISVHELVQEMNLGPI--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 615
           FE++GNR I + EL  E+ LGP    H+ L +W R +DGKLSF G+ + LHGV+ R
Sbjct: 597 FEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGVSSR 596

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7016799.10.0e+00100.00CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022922250.10.0e+0090.65CDPK-related kinase 4 isoform X2 [Cucurbita moschata][more]
XP_023549645.10.0e+0088.64CDPK-related kinase 6-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022974330.11.2e-30888.16CDPK-related kinase 6-like isoform X2 [Cucurbita maxima][more]
KAG6579299.17.3e-29390.33CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9FIM99.5e-20357.12CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1[more]
Q9SG121.8e-20157.21CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1[more]
Q9ZUZ21.5e-20061.17CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1[more]
Q9SCS21.4e-17755.20CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1[more]
P536812.6e-17653.21CDPK-related protein kinase OS=Daucus carota OX=4039 GN=CRK PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1E8740.0e+0090.65CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... [more]
A0A6J1IDP06.0e-30988.16CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11147296... [more]
A0A6J1E2V04.8e-29089.64CDPK-related kinase 4 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430287 P... [more]
A0A6J1IHA71.1e-28187.16CDPK-related kinase 6-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11147296... [more]
A0A6J1K6B22.0e-28079.15CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G24430.16.7e-20457.12Calcium-dependent protein kinase (CDPK) family protein [more]
AT3G49370.11.3e-20257.21Calcium-dependent protein kinase (CDPK) family protein [more]
AT2G46700.11.1e-20161.17CDPK-related kinase 3 [more]
AT3G50530.23.3e-18256.04CDPK-related kinase [more]
AT3G50530.19.8e-17955.20CDPK-related kinase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.238.10coord: 449..602
e-value: 5.0E-16
score: 61.0
NoneNo IPR availableGENE3D1.10.238.10coord: 471..539
e-value: 5.0E-16
score: 61.0
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 208..448
e-value: 3.1E-54
score: 185.9
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 110..207
e-value: 2.0E-22
score: 81.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..68
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 7..45
NoneNo IPR availablePANTHERPTHR24349SERINE/THREONINE-PROTEIN KINASEcoord: 39..298
coord: 329..614
NoneNo IPR availablePANTHERPTHR24349:SF361CDPK-RELATED KINASE 3coord: 329..614
NoneNo IPR availablePANTHERPTHR24349:SF361CDPK-RELATED KINASE 3coord: 39..298
NoneNo IPR availableCDDcd05117STKc_CAMKcoord: 116..431
e-value: 1.16726E-111
score: 333.289
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 117..432
e-value: 1.8E-61
score: 207.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 117..432
score: 46.406883
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 123..149
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 464..610
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 116..445

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26781-RACarg26781-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0018105 peptidyl-serine phosphorylation
biological_process GO:0046777 protein autophosphorylation
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0009931 calcium-dependent protein serine/threonine kinase activity
molecular_function GO:0005516 calmodulin binding
molecular_function GO:0004683 calmodulin-dependent protein kinase activity
molecular_function GO:0004672 protein kinase activity