Carg26540 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26540
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ubiquitin carboxyl-terminal hydrolase 15
Location	Carg_Chr12: 9180576 .. 9181159 (-)
RNA-Seq Expression	Carg26540
Synteny	Carg26540

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATTCCACTTTCTCGACTTGAAAATCTTCTCAAAACCTCTCGAATTTCTCCATTTTTCTCAGAATGTTGCGTTATGGCGTCGTATTTCGCCTCATTTTTGTGCTGTTTCTTGCCCACGACGCCATTGAAGCGACCTCAAACCACGTGAGTAATCGAAAATCAGAACCTCATTTGGTTCCGATTTGCTACAAACGATTAGTTCAGTGAGTTGATTATGTGTTTTTTTGTTGTCTTTTAGGGTAATCGGTGGTTCGATCAAGAACCGATGATGGTGCCATTAATGGAGAACGTTGAGACGATGACGGCGGTGGCGATGAATGGCAATCGGCGGCAGCTGAGGAGCTTCCAGGTTTGTGCTCTCTGTACCTGCTGCGGCGGCGCTAGAGGAATGTGCCTTCCTTCTCCCTGTTGCTATGCCATAAACTGTAACATTCCTAATCGGCCCTTTGGCTTTTGCTCGTTTACTCCCAAGACCTGTAACTGCTTTGGCTGCCATATTTGAGCTTAGTTCTTATATCTATCTCTGTTTATGACATTGAAAATTGTTCTTAATTGTTCTTAATTGTTCTTAATTGTTCTTAAT

mRNA sequence

AAATTCCACTTTCTCGACTTGAAAATCTTCTCAAAACCTCTCGAATTTCTCCATTTTTCTCAGAATGTTGCGTTATGGCGTCGTATTTCGCCTCATTTTTGTGCTGTTTCTTGCCCACGACGCCATTGAAGCGACCTCAAACCACGGTAATCGGTGGTTCGATCAAGAACCGATGATGGTGCCATTAATGGAGAACGTTGAGACGATGACGGCGGTGGCGATGAATGGCAATCGGCGGCAGCTGAGGAGCTTCCAGGTTTGTGCTCTCTGTACCTGCTGCGGCGGCGCTAGAGGAATGTGCCTTCCTTCTCCCTGTTGCTATGCCATAAACTGTAACATTCCTAATCGGCCCTTTGGCTTTTGCTCGTTTACTCCCAAGACCTGTAACTGCTTTGGCTGCCATATTTGAGCTTAGTTCTTATATCTATCTCTGTTTATGACATTGAAAATTGTTCTTAATTGTTCTTAATTGTTCTTAATTGTTCTTAAT

Coding sequence (CDS)

ATGTTGCGTTATGGCGTCGTATTTCGCCTCATTTTTGTGCTGTTTCTTGCCCACGACGCCATTGAAGCGACCTCAAACCACGGTAATCGGTGGTTCGATCAAGAACCGATGATGGTGCCATTAATGGAGAACGTTGAGACGATGACGGCGGTGGCGATGAATGGCAATCGGCGGCAGCTGAGGAGCTTCCAGGTTTGTGCTCTCTGTACCTGCTGCGGCGGCGCTAGAGGAATGTGCCTTCCTTCTCCCTGTTGCTATGCCATAAACTGTAACATTCCTAATCGGCCCTTTGGCTTTTGCTCGTTTACTCCCAAGACCTGTAACTGCTTTGGCTGCCATATTTGA

Protein sequence

MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQLRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI

Homology

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BLAST of Carg26540 vs. NCBI nr
Match: XP_022937710.1 (uncharacterized protein LOC111444031 [Cucurbita moschata] >KAG7020979.1 hypothetical protein SDJN02_17667 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 250.4 bits (638), Expect = 7.6e-63
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

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BLAST of Carg26540 vs. NCBI nr
Match: KAG6586147.1 (hypothetical protein SDJN03_18880, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 247.3 bits (630), Expect = 6.4e-62
Identity = 113/114 (99.12%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           MLRYGVVFRLIFVLFLAHDAIEATSNHGNRW DQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg26540 vs. NCBI nr
Match: XP_023536878.1 (uncharacterized protein LOC111798131 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 246.1 bits (627), Expect = 1.4e-61
Identity = 112/114 (98.25%), Postives = 113/114 (99.12%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           MLRYGVVFRLIFVLFLAHDAIEATSNHGNRW DQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           +SFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  KSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg26540 vs. NCBI nr
Match: XP_022965498.1 (uncharacterized protein LOC111465383 [Cucurbita maxima])

HSP 1 Score: 242.3 bits (617), Expect = 2.1e-60
Identity = 111/114 (97.37%), Postives = 111/114 (97.37%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           M  YGVVFRLIFVLFLAHDAIEATSNHGNRW DQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MSPYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg26540 vs. NCBI nr
Match: XP_023546744.1 (uncharacterized protein LOC111805758 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 196.4 bits (498), Expect = 1.3e-46
Identity = 92/114 (80.70%), Postives = 102/114 (89.47%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           M  YGV+F LIF+LFLAH+AIEATS H N   DQEPMMVPLME +ET TA+ +NG RRQL
Sbjct: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVND-SDQEPMMVPLMEKMET-TAMVINGGRRQL 71

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQ+CALCTCCGGARG+C+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 72  RSFQICALCTCCGGARGVCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 123

[Close]

BLAST of Carg26540 vs. ExPASy TrEMBL
Match: A0A6J1FHK2 (uncharacterized protein LOC111444031 OS=Cucurbita moschata OX=3662 GN=LOC111444031 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.7e-63
Identity = 114/114 (100.00%), Postives = 114/114 (100.00%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg26540 vs. ExPASy TrEMBL
Match: A0A6J1HP40 (uncharacterized protein LOC111465383 OS=Cucurbita maxima OX=3661 GN=LOC111465383 PE=4 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 1.0e-60
Identity = 111/114 (97.37%), Postives = 111/114 (97.37%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           M  YGVVFRLIFVLFLAHDAIEATSNHGNRW DQEPMMVPLMENVETMTAVAMNGNRRQL
Sbjct: 1   MSPYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg26540 vs. ExPASy TrEMBL
Match: A0A6J1D9H7 (uncharacterized protein LOC111018147 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018147 PE=4 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 3.8e-44
Identity = 93/115 (80.87%), Postives = 98/115 (85.22%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETM-TAVAMNGNRRQ 60
           M  +GV F LIFVLFLA +AIEATS + N   DQEPMMVPLM   E M TAV M+G RR+
Sbjct: 2   MPSHGVAFSLIFVLFLAQNAIEATSIYVN-VSDQEPMMVPLMGLGEKMETAVVMSGGRRR 61

Query: 61  LRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           LRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 62  LRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115

[Close]

BLAST of Carg26540 vs. ExPASy TrEMBL
Match: A0A0A0LJJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G074210 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 2.5e-43
Identity = 85/114 (74.56%), Postives = 93/114 (81.58%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60
           M  YGV+F  I +LFLA +AIE TS H N   DQEP+MVPLME   T   +  +G RRQL
Sbjct: 1   MSSYGVLFSFILLLFLAQNAIEGTSIHVNE-SDQEPIMVPLMETTTTTAMMMNSGGRRQL 60

Query: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           +SFQ+CALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  KSFQICALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 113

[Close]

BLAST of Carg26540 vs. ExPASy TrEMBL
Match: A0A5D3C6S7 (Ubiquitin carboxyl-terminal hydrolase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold105G00010 PE=4 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.0e-42
Identity = 88/115 (76.52%), Postives = 95/115 (82.61%), Query Frame = 0


Query: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMN-GNRRQ 60
           M  YGV+F  IF+L LA +AIE TS   N   DQEP+MVPLME   T TA+ MN G RRQ
Sbjct: 1   MSSYGVLFSFIFLLILAQNAIEGTSILVNE-SDQEPIMVPLMET--TTTAMMMNSGGRRQ 60

Query: 61  LRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           L+SFQ+CALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI
Sbjct: 61  LKSFQICALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 112

[Close]

BLAST of Carg26540 vs. TAIR 10
Match: AT1G65295.1 (unknown protein; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G01015.1); Has 90 Blast hits to 90 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 90; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 141.7 bits (356), Expect = 3.5e-34
Identity = 67/107 (62.62%), Postives = 79/107 (73.83%), Query Frame = 0


Query: 10  LIFVLFL--AHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQLRSFQVCA 69
           L+F +F+  +H A E     G    D E MMVPL E       + MN  RR+L SFQ+C+
Sbjct: 17  LLFSIFISSSHQANEVLPAEG----DNEMMMVPLGEE----KFMVMNETRRKLGSFQICS 76

Query: 70  LCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           +CTCCGGA+G+CLPSPCCYAINCNIPNRPFG+CSFTPKTCNCFGCHI
Sbjct: 77  VCTCCGGAKGLCLPSPCCYAINCNIPNRPFGYCSFTPKTCNCFGCHI 115

[Close]

BLAST of Carg26540 vs. TAIR 10
Match: AT5G01015.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G65295.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 66.2 bits (160), Expect = 1.9e-11
Identity = 25/58 (43.10%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 57  RRQLRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115
           R+ +   ++C  C CC      C   PCC+ INC +PN+PFG C+F PK+C+C  C I
Sbjct: 49  RKLMTPLELCLECKCCSST--TCATMPCCFGINCQLPNKPFGVCAFVPKSCHCNSCSI 104

The following BLAST results are available for this feature:

BLAST of Carg26540 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022937710.1	7.6e-63	100.00	uncharacterized protein LOC111444031 [Cucurbita moschata] >KAG7020979.1 hypothet...	[more]
KAG6586147.1	6.4e-62	99.12	hypothetical protein SDJN03_18880, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023536878.1	1.4e-61	98.25	uncharacterized protein LOC111798131 [Cucurbita pepo subsp. pepo]	[more]
XP_022965498.1	2.1e-60	97.37	uncharacterized protein LOC111465383 [Cucurbita maxima]	[more]
XP_023546744.1	1.3e-46	80.70	uncharacterized protein LOC111805758 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg26540 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg26540 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FHK2	3.7e-63	100.00	uncharacterized protein LOC111444031 OS=Cucurbita moschata OX=3662 GN=LOC1114440...	[more]
A0A6J1HP40	1.0e-60	97.37	uncharacterized protein LOC111465383 OS=Cucurbita maxima OX=3661 GN=LOC111465383...	[more]
A0A6J1D9H7	3.8e-44	80.87	uncharacterized protein LOC111018147 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0LJJ1	2.5e-43	74.56	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G074210 PE=4 SV=1	[more]
A0A5D3C6S7	8.0e-42	76.52	Ubiquitin carboxyl-terminal hydrolase 15 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]

BLAST of Carg26540 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65295.1	3.5e-34	62.62	unknown protein; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structu...	[more]
AT5G01015.1	1.9e-11	43.10	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33786:SF2	UBIQUITIN CARBOXYL-TERMINAL HYDROLASE	coord: 4..114
None	No IPR available	PANTHER	PTHR33786	FAMILY NOT NAMED	coord: 4..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26540-RA	Carg26540-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity