Carg26539 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26539
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Tryptophan synthase
Location	Carg_Chr18: 1745312 .. 1747633 (-)
RNA-Seq Expression	Carg26539
Synteny	Carg26539

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTATTTTGTTCGAGGGAAGGATTGTGCTCAAAATGGACATTATCATGATATGAATAGAGTTGTGATTCCTACAATAGTATTCGTGATTAATTTGGGTGTTGTGGCTGTGCAGGAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAAGTATCTACAAGACGAGGAGGAAAAGGCCCCGAGATTTACAAAAAGAGAGAGGATCTGAACCATTGCGGTGCACACAAGATGAACAATGCAATAGCGCAGTTATGATTGCAAGCGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTTGGGGCTGGCCAGCATGGTGGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGCTGCTCTGACTATTCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGTGCTCAGGTACTCCCATTAATTTGTCAAAAGTATTGTTGGAAGGGAATCCAAATCGACTAATTAGGAATGATCATGAATTTATACAAAGAATATATGTCCATGATGTATGAGGCCTTTTTTGGATGTCCAAAGCCACGAGAGCTTTATGCTCAAAGTGGACAATATCATATATTATCATCTCGTGGAGATTCGTGATTCCTAACAGATTTAATATTCAGGTGTAAAAATCTGTAGAAGGAAACTTCAAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATAGAAGAAGAAGATGTGAGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGTTACCTTTTGCAAGATGAGGAAGGCCAAATCCTTGTTCCTCATTCCGTTGGTATAGGGTAAGTATGTTAGAAAGCCTCGTACTAATGGATATGTATTTCTTAGGTAAGTATGTTAGAAAGCCTCGTACTAATGAATATGTATTTCTTACTTATAAACTCATTATAAACCTCTTAATTAGCCGATGTGGCACTCATCTCCCAACAATCCTCAACAATCCTCAACAATCTTCCCCTCAGATAAAGTACACCATAGAGATTCCCCTGAGACCTATGGAATCCTCGAACAGCCTCCTCATCCGGAGCTCTCAACAATTCTTCCCTCAAATAAAGTACACTATAGAGCCTTCTCTGAAGTCTATGGAACTCTCAAACAACCTCCCTTTAATTCTCTCCTCAAAGAAAGTACACCATAGAACCTCATTTGAGGGCCTGCGAAACCCTCGAACAATCTCTCCTTAATCCTCCCCTCAATTAAAGTATACCATAGAGCTTCCTCTAAGGCCTATGGAACCCTCGAACAACCTCCCCTTAATCGAGGCTCGACTCCTTCTCTGGAGCCCCTCAAAGATCCTCTCCTCGGATGAAGTACACCATAAAGCTTCTCTTGAGGCTTATAGAGCCCTTGAACAGCCTTTCCTTAATCAAGACTCAACTCCTTTGGGGCCCTCGAACAAAATACACCATTTGTTCAACATTTAGTCACTTTTTACTACACTTTCAAGGCTCACAACTTCTTTATTCGACATTTGAGAATTCTATTGACATGGCTAAGTTAAGGACATGGCTCTGATACCGTGTTAGAAATCACGAATCTCCACAATTGTATGATATTATCCACTTTGAGTATTACCAATGAAGATGTATTCCTTACTTATAAACCCATGATCAATTTCTTAATTAGCCGATATGGGACTCTTCAACAATCTATTTTAAATTTCGAACTTTTAAGTTGAAGAACATGCAAATTTTGAATTGCAGACTGGAGTACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGTAGAGCTGAATTTCACACAGCAATAGACAAAGAAGCGGTGGAAGCTTACAAACGGGTATGCAGATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTTGACAAGCTTTGCCCCACCCTAACTGACGGTTGCAAAGTGGTGGTCAACTGTAGCGGTCGTGGTGATAAAGATGCTGCAATTGTTTTCAACTATGGGCACCACCAAGAGTGCTAG

mRNA sequence

ATGGTGTATTTTGTTCGAGGGAAGGATTGTGCTCAAAATGGACATTATCATGATATGAATAGAGTTGTGATTCCTACAATAGTATTCGTGATTAATTTGGGTGTTGTGGCTGTGCAGGAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAACGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTTGGGGCTGGCCAGCATGGTGGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGCTGCTCTGACTATTCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGTGCTCAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATAGAAGAAGAAGATGTGAGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGTTACCTTTTGCAAGATGAGGAAGGCCAAATCCTTGTTCCTCATTCCGTTGGTATAGGACTGGAGTACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGTAGAGCTGAATTTCACACAGCAATAGACAAAGAAGCGGTGGAAGCTTACAAACGGGTATGCAGATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTTGACAAGCTTTGCCCCACCCTAACTGACGGTTGCAAAGTGGTGGTCAACTGTAGCGGTCGTGGTGATAAAGATGCTGCAATTGTTTTCAACTATGGGCACCACCAAGAGTGCTAG

Coding sequence (CDS)

ATGGTGTATTTTGTTCGAGGGAAGGATTGTGCTCAAAATGGACATTATCATGATATGAATAGAGTTGTGATTCCTACAATAGTATTCGTGATTAATTTGGGTGTTGTGGCTGTGCAGGAGGAGCTGGAGGTGGCGCTGAGGGACTTTGTTGGGCGAGAGACGCCGCTGTATCACGCTGAGAGGCTGACAACGCATGGGGCGGAGGCGTGTGGTTGCCGCCACTTGGGGCTGGCCAGCATGGTGGTTGCCACCGCAGCTGCCTGCGCCAAACATGATTTAGAGTGCACCATTTTGATGGCTGCTCTGACTATTCAAGAAGCAATCCTCAAATGTTGTCTTAATCAAGCTACTCGTGCTCAGGATGCGTCGTCGGAGGCGATAAGAGAGTGGGTCGGGGACTTGGAAACGAGCTATTACTTGACGGGGACAGTGGTAGGGCCGCACCCATGTCCAGCTATGGTGAGGGAGTTTCAGTCAGTGATAGGAAAAGAGACGAGAAAACAAGCCATGGAGAAATGGGGAGGCTTACCAGACGTGCTTGTGGCATGCATAGGGAGTGGCTCAAACGCGTTGGGACTGTTCAATGAGTTCATAGAAGAAGAAGATGTGAGGTTGATTGGAGTTGAGGCTGCTGGGTTTGGATTGGATAGTGGAAAGCACTCAGCTACTTTGTCTAAAGGACATGTTGGGGTTTACCATGGAGCTTTGAGTTACCTTTTGCAAGATGAGGAAGGCCAAATCCTTGTTCCTCATTCCGTTGGTATAGGACTGGAGTACCCAGGAGTAGGACCAGAGCTGAGCTTCTTAAAAGAGAGTGGTAGAGCTGAATTTCACACAGCAATAGACAAAGAAGCGGTGGAAGCTTACAAACGGGTATGCAGATTGGAAGGCATTTTTCCATCATTGGAAGCTTCTCATGCCTTTGCTTATCTTGACAAGCTTTGCCCCACCCTAACTGACGGTTGCAAAGTGGTGGTCAACTGTAGCGGTCGTGGTGATAAAGATGCTGCAATTGTTTTCAACTATGGGCACCACCAAGAGTGCTAG

Protein sequence

MVYFVRGKDCAQNGHYHDMNRVVIPTIVFVINLGVVAVQEELEVALRDFVGRETPLYHAERLTTHGAEACGCRHLGLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC

Homology

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BLAST of Carg26539 vs. NCBI nr
Match: KAG7012380.1 (Tryptophan synthase beta chain 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 712.2 bits (1837), Expect = 2.2e-201
Identity = 348/348 (100.00%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 1   MVYFVRGKDCAQNGHYHDMNRVVIPTIVFVINLGVVAVQEELEVALRDFVGRETPLYHAE 60
           MVYFVRGKDCAQNGHYHDMNRVVIPTIVFVINLGVVAVQEELEVALRDFVGRETPLYHAE
Sbjct: 1   MVYFVRGKDCAQNGHYHDMNRVVIPTIVFVINLGVVAVQEELEVALRDFVGRETPLYHAE 60

Query: 61  RLTTHGAEACGCRHLGLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 120
           RLTTHGAEACGCRHLGLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ
Sbjct: 61  RLTTHGAEACGCRHLGLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 120

Query: 121 DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVL 180
           DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVL
Sbjct: 121 DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVL 180

Query: 181 VACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLL 240
           VACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLL
Sbjct: 181 VACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVYHGALSYLL 240

Query: 241 QDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRVCRLEGIFP 300
           QDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRVCRLEGIFP
Sbjct: 241 QDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRVCRLEGIFP 300

Query: 301 SLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 349
           SLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC
Sbjct: 301 SLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 348

[Close]

BLAST of Carg26539 vs. NCBI nr
Match: KAG6573205.1 (Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 527.3 bits (1357), Expect = 9.8e-146
Identity = 277/357 (77.59%), Postives = 286/357 (80.11%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLYHAERLT +       G E          CG   +       
Sbjct: 104 QEELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 163

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTILM +  I++      L +   AQ
Sbjct: 164 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQ 223

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVG+LETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 224 VKSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 283

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLPDVLVACIGSGSNALGLFNEF++EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 284 KWGGLPDVLVACIGSGSNALGLFNEFMKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 343

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 344 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 403

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 349
           VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC
Sbjct: 404 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 460

[Close]

BLAST of Carg26539 vs. NCBI nr
Match: XP_022954968.1 (tryptophan synthase beta chain 1-like [Cucurbita moschata])

HSP 1 Score: 521.2 bits (1341), Expect = 7.0e-144
Identity = 273/357 (76.47%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLY+AERLT H       G E          CG   +       
Sbjct: 104 QEELEVALRDFVGRETPLYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 163

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTI M +  I++      L +   AQ
Sbjct: 164 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQ 223

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 224 VKSVEGNFKDASSEAIREWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 283

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLPDVLVACIGSGSNALGLFNEFI EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 284 KWGGLPDVLVACIGSGSNALGLFNEFINEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 343

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 344 YHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 403

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 349
           VCRLEGIFPSLEASHAFAYLD+LCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC
Sbjct: 404 VCRLEGIFPSLEASHAFAYLDELCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 460

[Close]

BLAST of Carg26539 vs. NCBI nr
Match: XP_023542665.1 (tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 3.5e-143
Identity = 271/357 (75.91%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLYHAERLT +       G E          CG   +       
Sbjct: 104 QEELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 163

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTI M +  I++      L +   AQ
Sbjct: 164 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQ 223

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 224 VKSVEGNFKDASSEAIREWVGNLEMSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 283

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLP+VLVACIGSGSNALGLFNEF+ EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 284 KWGGLPEVLVACIGSGSNALGLFNEFMNEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 343

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 344 YHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 403

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 349
           VC+LEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC
Sbjct: 404 VCKLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 460

[Close]

BLAST of Carg26539 vs. NCBI nr
Match: KAG7012379.1 (Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 512.7 bits (1319), Expect = 2.5e-141
Identity = 270/355 (76.06%), Postives = 281/355 (79.15%), Query Frame = 0


Query: 40  EELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL-------- 99
           EELEVALRDFVGRETPLYHAERLT +       G E          CG   +        
Sbjct: 1   EELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQVM 60

Query: 100 --------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ- 159
                         G     VATAAACAKHDLECTILM +  I++      L +   AQ 
Sbjct: 61  IAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSDDIKKQSSNVVLIKLLGAQV 120

Query: 160 --------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 219
                   DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK
Sbjct: 121 KSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEK 180

Query: 220 WGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 279
           WGGLPDVLVACIGSGSNALGLFNEFI+EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY
Sbjct: 181 WGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGVY 240

Query: 280 HGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRV 339
           HGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGR EFHTAIDKEAVEAYKRV
Sbjct: 241 HGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRGEFHTAIDKEAVEAYKRV 300

Query: 340 CRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 348
           C+LEGIFPSLEASHAFAYLDKLCPTL DGCKVVVNCSGRGDKDAAIVFNY H+QE
Sbjct: 301 CKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNYQHYQE 355

[Close]

BLAST of Carg26539 vs. ExPASy Swiss-Prot
Match: O50046 (Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=16922 GN=TSB PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.3e-100
Identity = 189/347 (54.47%), Postives = 242/347 (69.74%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-----------------------------GAEA 98
           Q+EL+  L+D+VGRE+PLY AERLT H                              A+A
Sbjct: 110 QKELDGILKDYVGRESPLYFAERLTLHYKRPNGEGPEIYLKREDLNHTGAHKINNAVAQA 169

Query: 99  CGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKC----CLNQA 158
              + LG   ++         VATA  CA+  L+C I M A  ++   L       L   
Sbjct: 170 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCVIYMGAQDMERQALNVFRMRLLGAE 229

Query: 159 TRA--------QDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
            RA        +DA+SEAIR+WV ++E+++Y+ G+V GPHP P MVREF +VIGKETRKQ
Sbjct: 230 VRAVHSGTATLKDATSEAIRDWVTNVESTHYILGSVAGPHPYPMMVREFHAVIGKETRKQ 289

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           A+EKWGG PDVLVAC+G GSNA+GLF+EF++++DVR+IGVEAAGFGLDSGKH+ATL+KG 
Sbjct: 290 ALEKWGGKPDVLVACVGGGSNAMGLFHEFVDDKDVRMIGVEAAGFGLDSGKHAATLTKGE 349

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 336
           VGV HGA+SYLLQD++GQI+ PHS+  GL+YPGVGPE SFLK+ GRAE++   D+EA+EA
Sbjct: 350 VGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDIGRAEYYCCTDEEALEA 409

[Close]

BLAST of Carg26539 vs. ExPASy Swiss-Prot
Match: P14671 (Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 365.2 bits (936), Expect = 8.5e-100
Identity = 189/354 (53.39%), Postives = 239/354 (67.51%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-----------------------------GAEA 98
           Q EL   L+D+VGRE+PLY AERLT H                              A+A
Sbjct: 114 QRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQA 173

Query: 99  CGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
              + LG   ++         VATA  CA+  LEC I M A  ++   L     +   A+
Sbjct: 174 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAE 233

Query: 159 ------------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
                       DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQ
Sbjct: 234 VRGVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQ 293

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           A+EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG 
Sbjct: 294 ALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGD 353

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HGA+SYLLQD++GQI+ PHS+  GL+YPGVGPE SF K+ GRAE+++  D+EA+EA
Sbjct: 354 VGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEA 413

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA AYL+KLCPTL+DG +VV+N SGRGDKD   V  Y
Sbjct: 414 FKRVSRLEGIIPALETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKY 467

[Close]

BLAST of Carg26539 vs. ExPASy Swiss-Prot
Match: P25269 (Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TSB2 PE=1 SV=2)

HSP 1 Score: 363.6 bits (932), Expect = 2.5e-99
Identity = 188/354 (53.11%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-----------------------------GAEA 98
           Q EL   L+D+VGRE+PLY AERLT H                              A+A
Sbjct: 119 QRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQA 178

Query: 99  CGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
              + LG   ++         VATA  CA+  L+C I M A  ++   L     +   A+
Sbjct: 179 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAE 238

Query: 159 ------------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
                       DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQ
Sbjct: 239 VRGVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQ 298

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           AMEKWGG PDVLVAC+G GSNA+GLF+EF+++ +VR+IGVEAAGFGLDSGKH+ATL+KG 
Sbjct: 299 AMEKWGGKPDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGD 358

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HGA+SYLLQD++GQI+ PHS+  GL+YPGVGPE SFLK+ GRAE+ +  D+EA+EA
Sbjct: 359 VGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEA 418

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA A+L+KLCPTL DG +VV+N SGRGDKD      Y
Sbjct: 419 FKRVSRLEGIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKY 472

[Close]

BLAST of Carg26539 vs. ExPASy Swiss-Prot
Match: P43283 (Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 3.2e-99
Identity = 187/354 (52.82%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTHGAEACG--------------------------- 98
           Q+EL+  L+D+VGRE+PLY AERLT H   A G                           
Sbjct: 33  QKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHRGAHKINNAVAQA 92

Query: 99  --CRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKC----CLNQA 158
              + LG   ++         VATA  CA+  L+C I M A  ++   L       L   
Sbjct: 93  LLAKRLGKQRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMKLLGAE 152

Query: 159 TRA--------QDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
            RA        +DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVREF  VIGKETR+Q
Sbjct: 153 VRAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQ 212

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           AM KWGG PDVLVAC+G GSNA+GLF+EF+E++DVRLIGVEAAG G+D+ KH+ATL+KG 
Sbjct: 213 AMHKWGGKPDVLVACVGGGSNAMGLFHEFVEDQDVRLIGVEAAGHGVDTDKHAATLTKGQ 272

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HG++SYLLQD++GQ++ PHS+  GL+YPGVGPE SFLK+ GRAE+ +  D+EA++A
Sbjct: 273 VGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDA 332

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA AYL+KLCPTL DG +VV+NCSGRGDKD      Y
Sbjct: 333 FKRVSRLEGIIPALETSHALAYLEKLCPTLPDGVRVVLNCSGRGDKDVHTASKY 386

[Close]

BLAST of Carg26539 vs. ExPASy Swiss-Prot
Match: P43284 (Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 GN=TSB2 PE=2 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 7.2e-99
Identity = 185/354 (52.26%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTHGAEACG--------------------------- 98
           Q+EL+  L+D+VGRE+PLY AERLT H   A G                           
Sbjct: 87  QKELDGILKDYVGRESPLYFAERLTEHYKRADGTGPLIYLKREDLNHTGAHKINNAVAQA 146

Query: 99  --CRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKC----CLNQA 158
              + LG   ++         VATA  C +  L+C I M A  ++   L       L   
Sbjct: 147 LLAKRLGKQRIIAETGAGQHGVATATVCRRFGLQCIIYMGAQDMERQALNVFRMRLLGAE 206

Query: 159 TRA--------QDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
            RA        +DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVREF  VIGKETR+Q
Sbjct: 207 VRAVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVREFHKVIGKETRRQ 266

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           AM+KWGG PDVLVAC+G GSNA+GLF+EF+E++DVRL+G+EAAG G+D+ KH+ATL+KG 
Sbjct: 267 AMDKWGGKPDVLVACVGGGSNAMGLFHEFVEDQDVRLVGLEAAGHGVDTDKHAATLTKGQ 326

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HG++SYLLQD++GQ++ PHS+  GL+YPGVGPE SFLK+ GRAE+ +  D+EA++A
Sbjct: 327 VGVLHGSMSYLLQDDDGQVIEPHSISAGLDYPGVGPEHSFLKDIGRAEYDSVTDQEALDA 386

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA AYL+KLCPTL DG +VVVNCSGRGDKD      Y
Sbjct: 387 FKRVSRLEGIIPALETSHALAYLEKLCPTLADGVRVVVNCSGRGDKDVHTASKY 440

[Close]

BLAST of Carg26539 vs. ExPASy TrEMBL
Match: A0A6J1GSL9 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.4e-144
Identity = 273/357 (76.47%), Postives = 283/357 (79.27%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLY+AERLT H       G E          CG   +       
Sbjct: 104 QEELEVALRDFVGRETPLYYAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 163

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTI M +  I++      L +   AQ
Sbjct: 164 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLIKLLGAQ 223

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVG+LE SYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 224 VKSVEGNFKDASSEAIREWVGNLERSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 283

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLPDVLVACIGSGSNALGLFNEFI EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 284 KWGGLPDVLVACIGSGSNALGLFNEFINEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 343

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 344 YHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 403

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 349
           VCRLEGIFPSLEASHAFAYLD+LCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC
Sbjct: 404 VCRLEGIFPSLEASHAFAYLDELCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQEC 460

[Close]

BLAST of Carg26539 vs. ExPASy TrEMBL
Match: A0A6J1GTV6 (Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1)

HSP 1 Score: 510.8 bits (1314), Expect = 4.6e-141
Identity = 270/351 (76.92%), Postives = 279/351 (79.49%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLYHAERLT +       G E          CG   +       
Sbjct: 103 QEELEVALRDFVGRETPLYHAERLTKYYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 162

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTILM +  I++      L +   AQ
Sbjct: 163 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTILMGSEDIKKQSSNVVLIKLLGAQ 222

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 223 VKSVEGNFKDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 282

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLPDVLVACIGSGSNALGLFNEFI+EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 283 KWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 342

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQILVPHSVG+GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 343 YHGALSYLLQDDEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 402

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           VCRLEGIFPSLEASHAFAYLDKLCPTL DGCKVVVNCSGRGDKDAAIVFNY
Sbjct: 403 VCRLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 453

[Close]

BLAST of Carg26539 vs. ExPASy TrEMBL
Match: A0A6J1K0T4 (Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1)

HSP 1 Score: 504.6 bits (1298), Expect = 3.3e-139
Identity = 267/351 (76.07%), Postives = 275/351 (78.35%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CGCRHL------- 98
           QEELEVALRDFVGRETPLYHAERLT H       G E          CG   +       
Sbjct: 103 QEELEVALRDFVGRETPLYHAERLTRHYKDEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 162

Query: 99  ---------------GLASMVVATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
                          G     VATAAACAKHDLECTI M +  I++      L     AQ
Sbjct: 163 MIAKRMGRRRVVAATGAGQHGVATAAACAKHDLECTIFMGSEDIKKQSSNVVLINLLGAQ 222

Query: 159 ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                    DASSEAIREWVG+LE SYYL GTVVGPHPCPAMVREFQSVIGKETRKQAME
Sbjct: 223 VKSVEGNFKDASSEAIREWVGNLEMSYYLMGTVVGPHPCPAMVREFQSVIGKETRKQAME 282

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWGGLPDVLVACIGSGSNALGLFNEFI+EEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 283 KWGGLPDVLVACIGSGSNALGLFNEFIKEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 342

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQDEEGQILVPHSVG+GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR
Sbjct: 343 YHGALSYLLQDEEGQILVPHSVGVGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 402

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           VC+LEGIFPSLEASHAFAYLDKLCPTL DGCKVVVNCSGRGDKDAAIVFNY
Sbjct: 403 VCKLEGIFPSLEASHAFAYLDKLCPTLRDGCKVVVNCSGRGDKDAAIVFNY 453

[Close]

BLAST of Carg26539 vs. ExPASy TrEMBL
Match: A0A5D3BMJ6 (Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005960 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.4e-129
Identity = 254/356 (71.35%), Postives = 274/356 (76.97%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CG----------- 98
           QEELEVALRDFVGRETPLY+AERLT H       G E          CG           
Sbjct: 101 QEELEVALRDFVGRETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 160

Query: 99  --CRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRA- 158
              + +G  S+V         VATAAACAKHDL+CTI M +  I +      L +   A 
Sbjct: 161 MIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDINKQSSNVLLIKLLGAK 220

Query: 159 --------QDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                   +DASSEAIREWVG+LETSYYLTGTVVGP+PCPAMVREFQSVIGKETR+QA E
Sbjct: 221 VKSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPYPCPAMVREFQSVIGKETRRQAKE 280

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWG  PDVL+ACIGSGSNALGLF+EFI E+DVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 281 KWGAKPDVLLACIGSGSNALGLFHEFINEKDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 340

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQIL PHSVG+GLEYPGVGPELSFLKESGRAEF TA D EAVEAYKR
Sbjct: 341 YHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPELSFLKESGRAEFETASDTEAVEAYKR 400

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 348
           + +LEGIFPSLEASHAFAYL KLCPTL DGCKVVVNCSGRGDKDAAIVFNY  HQ+
Sbjct: 401 LAKLEGIFPSLEASHAFAYLHKLCPTLPDGCKVVVNCSGRGDKDAAIVFNY--HQQ 454

[Close]

BLAST of Carg26539 vs. ExPASy TrEMBL
Match: A0A1S3B3A1 (Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 1.4e-129
Identity = 254/356 (71.35%), Postives = 274/356 (76.97%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-------GAEA---------CG----------- 98
           QEELEVALRDFVGRETPLY+AERLT H       G E          CG           
Sbjct: 100 QEELEVALRDFVGRETPLYYAERLTKHYKNEEGKGPEIYIKREDLNHCGAHKMNNAIAQV 159

Query: 99  --CRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRA- 158
              + +G  S+V         VATAAACAKHDL+CTI M +  I +      L +   A 
Sbjct: 160 MIAKRMGRKSVVAATGAGQHGVATAAACAKHDLDCTIFMGSEDINKQSSNVLLIKLLGAK 219

Query: 159 --------QDASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAME 218
                   +DASSEAIREWVG+LETSYYLTGTVVGP+PCPAMVREFQSVIGKETR+QA E
Sbjct: 220 VKSVEGNFKDASSEAIREWVGNLETSYYLTGTVVGPYPCPAMVREFQSVIGKETRRQAKE 279

Query: 219 KWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 278
           KWG  PDVL+ACIGSGSNALGLF+EFI E+DVRLIGVEAAGFGLDSGKHSATLSKGHVGV
Sbjct: 280 KWGAKPDVLLACIGSGSNALGLFHEFINEKDVRLIGVEAAGFGLDSGKHSATLSKGHVGV 339

Query: 279 YHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKR 338
           YHGALSYLLQD+EGQIL PHSVG+GLEYPGVGPELSFLKESGRAEF TA D EAVEAYKR
Sbjct: 340 YHGALSYLLQDDEGQILNPHSVGVGLEYPGVGPELSFLKESGRAEFETASDTEAVEAYKR 399

Query: 339 VCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYGHHQE 348
           + +LEGIFPSLEASHAFAYL KLCPTL DGCKVVVNCSGRGDKDAAIVFNY  HQ+
Sbjct: 400 LAKLEGIFPSLEASHAFAYLHKLCPTLPDGCKVVVNCSGRGDKDAAIVFNY--HQQ 453

[Close]

BLAST of Carg26539 vs. TAIR 10
Match: AT5G28237.1 (Pyridoxal-5'-phosphate-dependent enzyme family protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.5e-112
Identity = 214/355 (60.28%), Postives = 250/355 (70.42%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTHG-------------------------------- 98
           QEEL  ALRD+VGRETPLY AERLT H                                 
Sbjct: 105 QEELTTALRDYVGRETPLYFAERLTEHYKNIVPTIEGGPEIYLKREDLSHCGSHKINNAL 164

Query: 99  AEACGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQAT 158
           A+A   R LG + +V         VATAAACAK  LECT+ M A  I++        +  
Sbjct: 165 AQAMISRRLGCSRVVAATGAGQHGVATAAACAKLSLECTVFMGAADIEKQSFNVLSMKLL 224

Query: 159 RAQ---------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
            AQ         DASSEAIR WV +L T+YYL+GTVVGPHPCP +VREFQSVIGKETR+Q
Sbjct: 225 GAQVISVEGTFKDASSEAIRNWVENLYTTYYLSGTVVGPHPCPIIVREFQSVIGKETRRQ 284

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           A + WGG PDVLVAC+GSGSNALGLF+EF+ +EDVRL+GVEAAG GLDSGKHSATL+ G 
Sbjct: 285 AKQLWGGKPDVLVACVGSGSNALGLFHEFVGDEDVRLVGVEAAGLGLDSGKHSATLAFGD 344

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGVYHG++SYLLQD++GQIL PHSVG+GLEYPGVGPE+SF+KE+GRAEF+TA D+EA++A
Sbjct: 345 VGVYHGSMSYLLQDDQGQILKPHSVGVGLEYPGVGPEISFMKETGRAEFYTATDEEAIQA 404

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNYG 344
             R+ RLEGI P+LEASHA A+LDKL PTL DG KVVVNCSGRGDKD   +   G
Sbjct: 405 CMRLSRLEGIIPALEASHALAFLDKLVPTLRDGAKVVVNCSGRGDKDLDTLIQRG 459

[Close]

BLAST of Carg26539 vs. TAIR 10
Match: AT5G54810.1 (tryptophan synthase beta-subunit 1 )

HSP 1 Score: 365.2 bits (936), Expect = 6.0e-101
Identity = 189/354 (53.39%), Postives = 239/354 (67.51%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-----------------------------GAEA 98
           Q EL   L+D+VGRE+PLY AERLT H                              A+A
Sbjct: 114 QRELAGILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQA 173

Query: 99  CGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
              + LG   ++         VATA  CA+  LEC I M A  ++   L     +   A+
Sbjct: 174 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLECIIYMGAQDMERQALNVFRMRLLGAE 233

Query: 159 ------------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
                       DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQ
Sbjct: 234 VRGVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQ 293

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           A+EKWGG PDVLVAC+G GSNA+GLF+EF+ + +VR+IGVEAAGFGLDSGKH+ATL+KG 
Sbjct: 294 ALEKWGGKPDVLVACVGGGSNAMGLFHEFVNDTEVRMIGVEAAGFGLDSGKHAATLTKGD 353

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HGA+SYLLQD++GQI+ PHS+  GL+YPGVGPE SF K+ GRAE+++  D+EA+EA
Sbjct: 354 VGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFFKDMGRAEYYSITDEEALEA 413

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA AYL+KLCPTL+DG +VV+N SGRGDKD   V  Y
Sbjct: 414 FKRVSRLEGIIPALETSHALAYLEKLCPTLSDGTRVVLNFSGRGDKDVQTVAKY 467

[Close]

BLAST of Carg26539 vs. TAIR 10
Match: AT4G27070.1 (tryptophan synthase beta-subunit 2 )

HSP 1 Score: 363.6 bits (932), Expect = 1.8e-100
Identity = 188/354 (53.11%), Postives = 238/354 (67.23%), Query Frame = 0


Query: 39  QEELEVALRDFVGRETPLYHAERLTTH-----------------------------GAEA 98
           Q EL   L+D+VGRE+PLY AERLT H                              A+A
Sbjct: 119 QRELAEILKDYVGRESPLYFAERLTEHYRRENGEGPLIYLKREDLNHTGAHKINNAVAQA 178

Query: 99  CGCRHLGLASMV---------VATAAACAKHDLECTILMAALTIQEAILKCCLNQATRAQ 158
              + LG   ++         VATA  CA+  L+C I M A  ++   L     +   A+
Sbjct: 179 LLAKRLGKKRIIAETGAGQHGVATATVCARFGLQCIIYMGAQDMERQALNVFRMRLLGAE 238

Query: 159 ------------DASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQ 218
                       DA+SEAIR+WV ++ET++Y+ G+V GPHP P MVR+F +VIGKETRKQ
Sbjct: 239 VRGVHSGTATLKDATSEAIRDWVTNVETTHYILGSVAGPHPYPMMVRDFHAVIGKETRKQ 298

Query: 219 AMEKWGGLPDVLVACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKGH 278
           AMEKWGG PDVLVAC+G GSNA+GLF+EF+++ +VR+IGVEAAGFGLDSGKH+ATL+KG 
Sbjct: 299 AMEKWGGKPDVLVACVGGGSNAMGLFHEFVDDTEVRMIGVEAAGFGLDSGKHAATLTKGD 358

Query: 279 VGVYHGALSYLLQDEEGQILVPHSVGIGLEYPGVGPELSFLKESGRAEFHTAIDKEAVEA 338
           VGV HGA+SYLLQD++GQI+ PHS+  GL+YPGVGPE SFLK+ GRAE+ +  D+EA+EA
Sbjct: 359 VGVLHGAMSYLLQDDDGQIIEPHSISAGLDYPGVGPEHSFLKDVGRAEYFSVTDEEALEA 418

Query: 339 YKRVCRLEGIFPSLEASHAFAYLDKLCPTLTDGCKVVVNCSGRGDKDAAIVFNY 343
           +KRV RLEGI P+LE SHA A+L+KLCPTL DG +VV+N SGRGDKD      Y
Sbjct: 419 FKRVSRLEGIIPALETSHALAHLEKLCPTLPDGARVVLNFSGRGDKDVQTAIKY 472

[Close]

BLAST of Carg26539 vs. TAIR 10
Match: AT5G38530.1 (tryptophan synthase beta type 2 )

HSP 1 Score: 64.3 bits (155), Expect = 2.2e-10
Identity = 58/193 (30.05%), Postives = 85/193 (44.04%), Query Frame = 0


Query: 122 ASSEAIREWVGDLETSYYLTGTVVGPHPCPAMVREFQSVIGKETRKQAMEKWGGLPDVLV 181
           A SEA+     + +T Y L G+V+        V   Q++IG+E  +Q ME +G  PD+++
Sbjct: 263 AISEAVEVAARNEDTKYCL-GSVLN------HVLLHQTIIGEECIQQ-MENFGETPDLII 322

Query: 182 ACIGSGSNALGLFNEFIEEEDVRLIGVEAAGFGLDSGKHSATLSKG----HVGVYHGALS 241
            C G GSN  GL   FI E   +L G               +L+KG      G   G   
Sbjct: 323 GCTGGGSNFAGLSFPFIRE---KLKGKINPVIRAVEPSACPSLTKGVYAYDFGDTAGLTP 382

Query: 242 YLLQDEEGQILVPHSVGI-GLEYPGVGPELSFLKESGRAEFHTAIDKEAVEAYKRVCRLE 301
            +     G   +P  +   GL Y G+ P +S + E G  E  +    E  +   +  R E
Sbjct: 383 LMKMHTLGHDFIPDPIHAGGLRYHGMAPLISHVYEQGFMEAISIPQIECFQGAIQFARTE 442

Query: 302 GIFPSLEASHAFA 310
           GI P+ E +HA A
Sbjct: 443 GIIPAPEPTHAIA 444

The following BLAST results are available for this feature:

BLAST of Carg26539 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012380.1	2.2e-201	100.00	Tryptophan synthase beta chain 1, partial [Cucurbita argyrosperma subsp. argyros...	[more]
KAG6573205.1	9.8e-146	77.59	Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma...	[more]
XP_022954968.1	7.0e-144	76.47	tryptophan synthase beta chain 1-like [Cucurbita moschata]	[more]
XP_023542665.1	3.5e-143	75.91	tryptophan synthase beta chain 1-like [Cucurbita pepo subsp. pepo]	[more]
KAG7012379.1	2.5e-141	76.06	Tryptophan synthase beta chain 1, chloroplastic, partial [Cucurbita argyrosperma...	[more]

BLAST of Carg26539 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O50046	1.3e-100	54.47	Tryptophan synthase beta chain 2, chloroplastic OS=Camptotheca acuminata OX=1692...	[more]
P14671	8.5e-100	53.39	Tryptophan synthase beta chain 1, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P25269	2.5e-99	53.11	Tryptophan synthase beta chain 2, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
P43283	3.2e-99	52.82	Tryptophan synthase beta chain 1 (Fragment) OS=Zea mays OX=4577 GN=TSB1 PE=2 SV=...	[more]
P43284	7.2e-99	52.26	Tryptophan synthase beta chain 2, chloroplastic (Fragment) OS=Zea mays OX=4577 G...	[more]

BLAST of Carg26539 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GSL9	3.4e-144	76.47	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457067 PE=3 SV=1	[more]
A0A6J1GTV6	4.6e-141	76.92	Tryptophan synthase OS=Cucurbita moschata OX=3662 GN=LOC111457069 PE=3 SV=1	[more]
A0A6J1K0T4	3.3e-139	76.07	Tryptophan synthase OS=Cucurbita maxima OX=3661 GN=LOC111490065 PE=3 SV=1	[more]
A0A5D3BMJ6	1.4e-129	71.35	Tryptophan synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G...	[more]
A0A1S3B3A1	1.4e-129	71.35	Tryptophan synthase OS=Cucumis melo OX=3656 GN=LOC103485497 PE=3 SV=1	[more]

BLAST of Carg26539 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G28237.1	1.5e-112	60.28	Pyridoxal-5'-phosphate-dependent enzyme family protein	[more]
AT5G54810.1	6.0e-101	53.39	tryptophan synthase beta-subunit 1	[more]
AT4G27070.1	1.8e-100	53.11	tryptophan synthase beta-subunit 2	[more]
AT5G38530.1	2.2e-10	30.05	tryptophan synthase beta type 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 35..154 e-value: 3.8E-26 score: 93.9
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	GENE3D	3.40.50.1100		coord: 155..347 e-value: 1.2E-74 score: 252.6
IPR036052	Tryptophan synthase beta subunit-like PLP-dependent enzyme	SUPERFAMILY	53686	Tryptophan synthase beta subunit-like PLP-dependent enzymes	coord: 34..340
IPR023026	Tryptophan synthase beta chain/beta chain-like	PIRSF	PIRSF001413	Trp_syn_beta	coord: 34..69 e-value: 0.079 score: 10.0 coord: 80..347 e-value: 6.9E-77 score: 257.2
IPR001926	Pyridoxal-phosphate dependent enzyme	PFAM	PF00291	PALP	coord: 81..330 e-value: 1.9E-18 score: 66.9
None	No IPR available	PANTHER	PTHR48077	TRYPTOPHAN SYNTHASE-RELATED	coord: 79..346
None	No IPR available	PANTHER	PTHR48077	TRYPTOPHAN SYNTHASE-RELATED	coord: 36..65
None	No IPR available	PANTHER	PTHR48077:SF4	TRYPTOPHAN SYNTHASE	coord: 79..346 coord: 36..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26539-RA	Carg26539-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0000162	tryptophan biosynthetic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004834	tryptophan synthase activity