Carg26503 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26503
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Nitrilase/cyanide hydratase
Location	Carg_Chr13: 4839270 .. 4844755 (+)
RNA-Seq Expression	Carg26503
Synteny	Carg26503

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CGAACTATCTGAAAACCACTGGACTCCATTGCAGCCATTCATTCCACTCTCATTCTCTGCTCCAATCTCAAGCTCAAGTTCAAGCTAAGAGGGGAAAAGGGCGATTTCGAATCATCCATCCACCATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGGTTTCCTTTCCTCACGTACACTTTAATTTTATCGTTGTGTAGTTCTCTTACTGTTGTTTCTTTGCTAACCATCTCTTGGCTTGGGATGGTCATTTCAGAGTTTGGAATGAGTTCCTGGTTGTTTTTTGGTGTGATGTTAGAAGCTTATCAGTTGTTTGAATGTTTTGTTTACTCTATGAAGTTTCTTCCCTTCTATCTGCAGATTTGCGAATAATGATTTAATTCATTTAAGCTATTATATCCTCGATTTTTTTTTTTCGTTTTTCATTGGTTTTCCTGTTTGCGGTCAATGTGAATGTAACTGTCCGTCCACAACTAAAGATATGGTACTCTTTGGGCTTTCTTTAAAACATGTTTGGTAGGGAGGTTTCCATACCCTTATAAAGAATCCTCCGAACGTGGGATCTCAACAGTCCACCCCTTCAGGGCTTAACGTCCTCGTTAGCACTCGTTCTTTTTTCCAATTGATGTAGGACGTTAGGCCCCGAAGTGGGTAGATTGTGAGATCCCAAATAGCATCAAAGCTGACGATGTGCCAGCAAGGAGGCTGATCTCTAAAGAGGGTGGACACGAGACGGTGTGCTAGCAAGGACCTTGGGACCTGAAGGGGGTGGATTGGAGGGTCCCACATCGATTGGAGAAGGGAACAAGTGGCAGCAAGGATGCTGGGCCCCGAAAGAAGATGGATTGTGAGATCCTACATTGGTTGGGAAGGAGAACGAAACATTATTTAGAACAATGTGGAAATCTCTCTCTAGCAAACGCGTTTTAAAAACCTTGAAGAGAAGTCCGAAAAGAAAAGTCCTAAGAAGGCAATATCTACTAGCAGTGAGCTTGGGCCGTTACCATAAATTTGTCAATGTCACCTAACATTGTACTCCTTATTGTTTTTTTCTCCTTAATTTCAGTGGATTGGAGAAATTATTTAATTCATAAATTCCAAAAGCGATTGATAGAGTTGTGGATCATCTCACGTAGATAGCAACTATTTTAGTAGATTGCACGCCATCTACTAGCCTATGACCATGTGGGTGTTGTTTAACTTGTAGTGAAAAACTAATTCTGGTTTACATGAACAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTTAGTCTTTGTTTCCAATTCGACTTTGTTTAACTTAGTTGTGGTTGCATCTGTAAAGAGAACAATATTAGTGTCTTACCATGGATGATTTCAGGTTAAATGGACATTGTTAGGTGTAATAATAATACTCATTCTTGAAGGCCGACTTCGTTCCTCTACGAGTCTCTACCAAACTTTATTATATGGTCATATATTAATATCAAAGTCTTAAATCTTAGTATGCATCTTCTGTTCTTTATGAACTTTCAATCTTTAAGTAGATAATAGCTCATATTAGGACACTACAATAAGAACTTAGTGATGTCACTCTATTCCATCTGAAATACGAATCGGTTGTTCATGAAATGTGAAATGGGACTTTAGCCCTATTTTAATGGCTCCTGTAATTTACAATTGATGAATGAGAAACAATGCTTCAAAAATCTACAGGCATTTCCCGGGTCGAGTCTTTGCGAGTTCATCCCAAACAAAGCGAACCCGTTCTTTCTGGGTCTTGGTCAGGAAGATGTAGATAAAACGCCAATAATTTGATTCTTCTATGGCATAAGCTTTGAAGAAGAGAGTTATCAATCATCAGAAATTTCTCTTTTTTTCTCTCTTCCAAAATGAAACAAAATCCGTCATTAAATGACGAACCCCCTTATATAGATGATAGGACAAGGCTAAGGCAGTAATCTCGACGGAGATTAGAATGAACTTTGATGAATAAAAGACGAATCGGTAGAAATTCAAACCTTCCTACTCTTGCTTACACCTAAACATAATATTTTGAAGGTCTAAAAGCTATAGCCATTGTCTGTCTTAGGTGCTGCTTAGGTAGATGTAGTTCTAATTGAGTAAATGTTAATATGTTGATAACGATACATGCAGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGTTAGTTATTCTTGGCTTGAAATAATAAAATCCCTGACCTTCCCATTTTAAATCCAATTCCATTCATTTCGTTAGACTGTAAAGTGCTCATCAACTAAATTAGATGAAAATTTTCATTTAACAGAAGATTTTCCTATGGTTAAAGTTATACTGCTACCTTAACGATCAAAATTTTTACTTGAAAGTTATGTAACTTCCGTCCACACGTTCCTTTTCTTTTGCTCGACCTGTCGATCACTAGAAACTGACTTATATTTGTGGTCCTGTTTGTAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGGTATGTCATCACGTCCTTCTACTTATAGTCATTAGAGCTATAGTTATTTACTACGTTTAGTGGTTTATATAGAAAAATTGTTTAATCTTTAGGAAACCTACTATTTGATTTAAGCCACTTTTTGGGGTTCTTTCTCCTAGATGATCTAATACTCCTGTCGATCGAGTAGTATGAAAACCATCATTCTAAGCTCTTATTCTATGCTTAGCTCTTTTTTAGTTACAATACGAGTGAATATGTAACAGTCCAAACCCACCGCTAGCAAATATTGTCCTCTTTGGCATTTTCCTTTTGTGTTTTCCCTCAAGGTTTTTAAACACGGCTGATAGGGAGAGATTGTTGGGAATAAACTTAAATGAAAAGAAAAAGGTAGTTTTTTAATTTCTTTATTTTTAGTAAGGCTTTTGAAATTTGAGTCGTAGGAGAAGGAAAAGGCTAACAATAATTTTAGGAGAGAGCAAGAGTTACACTGTTATTAAAGTACAGTGTAATAGAAAAGGGTTTTGAGAATTTCTTTGTCTCCTTGTCTTTTCTAGAAGTTGGTATTGGAGCGACTTTTAATTTCAACAGAGATTTCCACTCTTATAAATAATATTTTGTTCCTCTCTCCAATCAATGTGGGATCTCACAATTCACTCCCTTTTGGGGCCAGCGTCCTTGCTGGCCCCGTTCCCTTCTCCAATCGATGTGGGACCTTTGTTCCTCTTTCCAACCGACGTGGAATCTCACATAATAGCTTGTCTTCCCTGGTACAAATTGATTTTCCAACCAAGTGGGTAAGTTATGATGAACCAACCAACCATTTAAGATAGGGATGGCCTATATGTCTCTTGCTAACTAACAAGTTCCTTGGAGAATGGGGAGATAATCCGAGGTAGTTTTGTTGAACTATCCATGCCTTGGGCTGTGAAACTTTTCCATCTTTTGTGTGAGCAAAGGTTGAACTTGAGATTCCTAAGGTAAGCCTCTTAATTAGGTTGAAGGGTTTTCCTTCACTTACTAAGGTTGGGATTCCTAAGGTAAGTCCAGCTGATTTCATATGATTTTAGGATTAGCCAATAGTCCCACTATTTACCAAAGATTCTACAATGTGCTATTCTATAAAGGGGAGCTACCCATTTAAACAAGCAACTATTGAATGGATGATGAGAAGATTGAATTATGAAGAGCAAGAATGCTCTATATATATACAAGAGAATACAAAAAGATATTTAGAAAATCTACCTAAAAATCTGGAGAAAATCTGGTAACAAACCAGTAAACAAATCTAATCAAATCAAATCAAAAGTAAATCTAACTCTAACGGAGATTTAATTAGATACTAAATGACAGCAATCTAAACAAATACGTGATCTTTAACATGGAACAATATGATATATTATTGATCCATCCCAACTCTTAACAAGTTAAACTTTTTGATTTTTGATTGTTATGGAAAGTTTTTGCTATGAATTGCTTTTATATATAATTTGGATGCATAACTGAAACAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAGTAAGTTTATTGTCGAAGCTTCAATTTAGTTCCATTAATTGTTGTAATCTACATGTGTTGTCTATGTTTTACTGCTTCCTCATGCGTTCAGTGTTGTATTATATTCATCCTAATCATTCAGAACTGTGAATATTTCTGAATTTATATCAGCGGTGGATTTTAATATCATTTTTTCCTTCATTTCTAGCCTAGTTTGATTCATGATACTGTATTTAGGTGGATCAAGATATTGGTATATTGATGTTAGTGTCTAGAGAGATGAATAGAAACTTGGCGGAAGATTATTATGATGGGAAAGGGCAAAGATCATCTGAATCACCCAAACTAATATGGATTGGTCTAGTGGTCGAAAATGTTATTGCTCAGAGAAAACCTACCTAGAATTTAATATCCTACCGGTTACGTAGGCAACTAAATGTAGTAGGGATAGAGTTGTATTATGAGACTAGTTGAGATGCGTACAAGCTGAGTTGGACACTCGTAGATGTCTAAAGCAAAATAATAATATTGCCTTGATTTAGTTCAATCATTCTCAACTATGAAACCAAAACTGGTTCGACTCATAATCAAATTGATATGGGTTGGTCTCAAAATAATA

mRNA sequence

CGAACTATCTGAAAACCACTGGACTCCATTGCAGCCATTCATTCCACTCTCATTCTCTGCTCCAATCTCAAGCTCAAGTTCAAGCTAAGAGGGGAAAAGGGCGATTTCGAATCATCCATCCACCATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAGTAAGTTTATTGTCGAAGCTTCAATTTAGTTCCATTAATTGTTGTAATCTACATGTGTTGTCTATGTTTTACTGCTTCCTCATGCGTTCAGTGTTGTATTATATTCATCCTAATCATTCAGAACTGTGAATATTTCTGAATTTATATCAGCGGTGGATTTTAATATCATTTTTTCCTTCATTTCTAGCCTAGTTTGATTCATGATACTGTATTTAGGTGGATCAAGATATTGGTATATTGATGTTAGTGTCTAGAGAGATGAATAGAAACTTGGCGGAAGATTATTATGATGGGAAAGGGCAAAGATCATCTGAATCACCCAAACTAATATGGATTGGTCTAGTGGTCGAAAATGTTATTGCTCAGAGAAAACCTACCTAGAATTTAATATCCTACCGGTTACGTAGGCAACTAAATGTAGTAGGGATAGAGTTGTATTATGAGACTAGTTGAGATGCGTACAAGCTGAGTTGGACACTCGTAGATGTCTAAAGCAAAATAATAATATTGCCTTGATTTAGTTCAATCATTCTCAACTATGAAACCAAAACTGGTTCGACTCATAATCAAATTGATATGGGTTGGTCTCAAAATAATA

Coding sequence (CDS)

ATGGCGCTTGTGCCAACCACGGCCGCCACCGATACCCAACTGATAGCGGAGGTGGATATGGGCTCCGATGTATCTGTACCGACTGTTCGAGCCACCGTTGTTCAGGCCTCCACCATCTTCTACGACACTCCTGCCACTCTAGATAAGGCCGAGAGGTTGTTGGCTGAAGCTGCTGGATTTGGATCCCAGCTAGTTCTGTTTCCAGAAGCCTTTGTTGGTGGTTATCCCCGTGGATCAAATTTTGGTGTTTCCATTGGGAATAGAACTCCCAAGGGGAAAGAGGAATTCCGTAAATACCACGCAGCAGCGATCGATGTACCTGGTCCTGAAGTCGATCGATTGGCAGCAATGGCTGGAAAGTATAGGGTGCATTTGGTAATGGGGGTAATAGAGAGAGATGGCTACACACTGTACTGTACTGTTCTATTTTTTGATCCTCAAGGTCGTTACTTGGGTAAGCACAGGAAAGTGATGCCAACTGCATTGGAGCGGATAATCTGGGGATTTGGTGATGGATCAACAATTCCTGTTTTTGAGACTTCAATTGGAAAGATAGGTGCTGCCATTTGCTGGGAAAACAGAATGCCACTCCTAAGAACGGCTATGTATGCCAAAGGCATTGAAATATATTGTGCACCTACTGCTGATTCTCGGGATACATGGCAAGCATCTATGACCCACATTGCTTTGGAGGGTGGTTGTTTTGTTTTGTCAGCCAACCAGTTCTGCCGAAGAAAAGACTACCCACCTCCACCAGATTATGTTTTTTCTGGCACAGAAGAGGAACTCAGCCCAGATTCTGTTGTTTGTGCTGGGGGTAGTGTCATCATATCGCCATCAGGTACAATCCTAGCTGGACCCAATTACGATGGAGAGGCACTTATTTCAGCAGATCTAGATCTTGGAGATATAGCACGGGCGAAATTTGATTTCGACGTGGTTGGTCATTATGCAAGGCCTGAAGTGCTTAGCTTGGTTGTGAGGGACCATCCTACAACCCCAGTTACTTTCACATCTGCAACTACAAAAACCGAAGATTCTTGTAAAAAATAG

Protein sequence

MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK

Homology

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: KAG6583706.1 (Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019353.1 Bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 711.1 bits (1834), Expect = 4.8e-201
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_023520715.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 708.4 bits (1827), Expect = 3.1e-200
Identity = 348/350 (99.43%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSA+TKTEDSCKK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASTKTEDSCKK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_022927562.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata])

HSP 1 Score: 704.1 bits (1816), Expect = 5.9e-199
Identity = 346/349 (99.14%), Postives = 347/349 (99.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSAT KTEDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_022973146.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima])

HSP 1 Score: 702.2 bits (1811), Expect = 2.2e-198
Identity = 345/350 (98.57%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSA+ KTEDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]

BLAST of Carg26503 vs. NCBI nr
Match: XP_004139500.1 (bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hypothetical protein Csa_022889 [Cucumis sativus])

HSP 1 Score: 686.8 bits (1771), Expect = 9.8e-194
Identity = 336/350 (96.00%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Carg26503 vs. ExPASy Swiss-Prot
Match: Q5QGZ8 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 GN=NIT4A PE=1 SV=1)

HSP 1 Score: 624.4 bits (1609), Expect = 7.8e-178
Identity = 299/349 (85.67%), Postives = 325/349 (93.12%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALV T    D  L AEV+M SD + PTVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVTTPTVNDGPLFAEVNMSSDFNAPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+Q+V+FPEAF+GGYPRGSNFGVSIGNRT KGKE+FRKYH+AAIDVPGPEVDRLAA+AGK
Sbjct: 61  GAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEDFRKYHSAAIDVPGPEVDRLAALAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFF  QGRYLGKHRK+MPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFGAQGRYLGKHRKLMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSR+ WQASMTHIALEGGCFVLSAN
Sbjct: 181 PIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSREVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRR+DYPPPP+YVF GTEE L+PDSVVCAGGSVIISPSG +LAGP+Y+GEALISADLD
Sbjct: 241 QFCRRRDYPPPPEYVFEGTEENLTPDSVVCAGGSVIISPSGAVLAGPSYEGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHY+RPEVLSLVV+DHPT PVTFTSA+TK ED  K
Sbjct: 301 LGEIARAKFDFDVVGHYSRPEVLSLVVKDHPTNPVTFTSASTKIEDKTK 349

[Close]

BLAST of Carg26503 vs. ExPASy Swiss-Prot
Match: Q42965 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=NIT4A PE=2 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 8.6e-177
Identity = 299/349 (85.67%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT A  +  L AEVDMG + S PTVRATVVQASTIFYDTPATL KAERLLAEAA +
Sbjct: 1   MALVPTPAVNEGPLFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLVKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLV+FPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG +LGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHFLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEE+L+PDS+VCAGGSVIISPSG +LAGPNY GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEDLTPDSIVCAGGSVIISPSGAVLAGPNYVGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTS ++K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 349

[Close]

BLAST of Carg26503 vs. ExPASy Swiss-Prot
Match: Q42966 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=NIT4B PE=2 SV=1)

HSP 1 Score: 619.0 bits (1595), Expect = 3.3e-176
Identity = 298/349 (85.39%), Postives = 324/349 (92.84%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPT    +  + AEVDMG + S PTVRATVVQASTIFYDTPATLDKAERLLAEAA +
Sbjct: 1   MALVPTPVVNEGPMFAEVDMGDNSSTPTVRATVVQASTIFYDTPATLDKAERLLAEAASY 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           G+QLV+FPEAF+GGYPRGS FGVSIGNRT KGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GAQLVVFPEAFIGGYPRGSTFGVSIGNRTAKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           Y+V+LVMGVIERDGYTLYCTVLFFD QG YLGKHRK+MPTALERIIWGFGDGSTIPV++T
Sbjct: 121 YKVYLVMGVIERDGYTLYCTVLFFDSQGHYLGKHRKIMPTALERIIWGFGDGSTIPVYDT 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
            +GKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRD WQASMTHIALEGGCFVLSAN
Sbjct: 181 PLGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDVWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGT E+L+PDS+VCAGGSVIISPSG +LAGPNY+GEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGT-EDLTPDSIVCAGGSVIISPSGAVLAGPNYEGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LG+IARAKFDFDVVGHYARPEVLSL+VRDH  +PV+FTS ++K E S K
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLIVRDHAVSPVSFTSTSSKAESSPK 348

[Close]

BLAST of Carg26503 vs. ExPASy Swiss-Prot
Match: Q3LRV4 (Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 GN=NIT4B PE=1 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 1.4e-174
Identity = 298/350 (85.14%), Postives = 322/350 (92.00%), Query Frame = 0


Query: 1   MALVPTT-AATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAG 60
           MALV TT    D  L AEVDM S  +  TVRATVVQASTIFYDTPATLDKAERLL +AA 
Sbjct: 1   MALVTTTPTVNDEPLFAEVDMASYFTSTTVRATVVQASTIFYDTPATLDKAERLLVQAAS 60

Query: 61  FGSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAG 120
           +G+Q+V+FPEAF+GGYPRGSNFGVSIGNRT KGKEEFRKYH+AAIDVPGPEVDRL+AMAG
Sbjct: 61  YGAQIVVFPEAFIGGYPRGSNFGVSIGNRTAKGKEEFRKYHSAAIDVPGPEVDRLSAMAG 120

Query: 121 KYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFE 180
           KY+V+LVMGVIERDGYTLYCTVLFFD QGRYLGKHRKVMPTALERIIWGFGDGSTIPVF+
Sbjct: 121 KYKVYLVMGVIERDGYTLYCTVLFFDSQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFQ 180

Query: 181 TSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSA 240
           T IGKIGAAICWEN+MPLLRTAMYAKG+EIYCAPTADSRD WQAS THIALEGGCFVLSA
Sbjct: 181 TPIGKIGAAICWENKMPLLRTAMYAKGVEIYCAPTADSRDLWQASTTHIALEGGCFVLSA 240

Query: 241 NQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADL 300
           NQFCRRKDYPPPP+YVFSGTEE+L+PDSVV AGGSVIISPSG +LAGPNY+GEALISADL
Sbjct: 241 NQFCRRKDYPPPPEYVFSGTEEDLTPDSVVSAGGSVIISPSGAVLAGPNYEGEALISADL 300

Query: 301 DLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           DLG+IARAKFDFDVVGHY+R EVLSL+V+DHPT PVTFTS +TK ED  K
Sbjct: 301 DLGEIARAKFDFDVVGHYSRSEVLSLIVKDHPTNPVTFTSTSTKIEDQTK 350

[Close]

BLAST of Carg26503 vs. ExPASy Swiss-Prot
Match: Q6H849 (Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX=39947 GN=NIT4 PE=2 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 1.6e-162
Identity = 281/350 (80.29%), Postives = 309/350 (88.29%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDM--GSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAA 60
           MA+VP+ +     +IAEV+M  G+D    TVRATVVQAST+FYDTPATLDKAERL+ EAA
Sbjct: 1   MAMVPSGSGGGPPVIAEVEMNGGADSGAATVRATVVQASTVFYDTPATLDKAERLIEEAA 60

Query: 61  GFGSQLVLFPEAFVGGYPRGSNFG----VSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRL 120
           G+GSQLV+FPEAFVGGYPRGS FG    +SIGN   KGKEEFRKYHAAAI+VPGPEV RL
Sbjct: 61  GYGSQLVVFPEAFVGGYPRGSTFGFGANISIGNPKDKGKEEFRKYHAAAIEVPGPEVTRL 120

Query: 121 AAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGST 180
           AAMAGKY+V LVMGVIER+GYTLYC+VLFFDP GRYLGKHRK+MPTALERIIWGFGDGST
Sbjct: 121 AAMAGKYKVFLVMGVIEREGYTLYCSVLFFDPLGRYLGKHRKLMPTALERIIWGFGDGST 180

Query: 181 IPVFETSIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGC 240
           IPV++T +GKIGA ICWEN+MPLLRTA+Y KGIEIYCAPTADSR  WQASMTHIALEGGC
Sbjct: 181 IPVYDTPLGKIGALICWENKMPLLRTALYGKGIEIYCAPTADSRQVWQASMTHIALEGGC 240

Query: 241 FVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEAL 300
           FVLSANQFCRRKDYPPPP+YVFSG  EE SPD+VVC GGSVIISPSG +LAGPNY+GEAL
Sbjct: 241 FVLSANQFCRRKDYPPPPEYVFSGLGEEPSPDTVVCPGGSVIISPSGEVLAGPNYEGEAL 300

Query: 301 ISADLDLGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKT 345
           I+ADLDLG+I RAKFDFDVVGHYARPEVLSLVV D P  PV+FTSA  KT
Sbjct: 301 ITADLDLGEIVRAKFDFDVVGHYARPEVLSLVVNDQPHLPVSFTSAAEKT 350

[Close]

BLAST of Carg26503 vs. ExPASy TrEMBL
Match: A0A6J1EHI6 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=LOC111434356 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 2.9e-199
Identity = 346/349 (99.14%), Postives = 347/349 (99.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAAADTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCK 350
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSAT KTEDSCK
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATAKTEDSCK 349

[Close]

BLAST of Carg26503 vs. ExPASy TrEMBL
Match: A0A6J1I7W0 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LOC111471660 PE=3 SV=1)

HSP 1 Score: 702.2 bits (1811), Expect = 1.1e-198
Identity = 345/350 (98.57%), Postives = 347/350 (99.14%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTAA DTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAANDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSA+ KTEDSC K
Sbjct: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSASKKTEDSCNK 350

[Close]

BLAST of Carg26503 vs. ExPASy TrEMBL
Match: A0A0A0LVI0 (CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G168360 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 4.7e-194
Identity = 336/350 (96.00%), Postives = 343/350 (98.00%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD+QLIAEVDMGSD SV  VRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDSQLIAEVDMGSDSSVMNVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHA+AIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHASAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSTSTKVEDSCKK 350

[Close]

BLAST of Carg26503 vs. ExPASy TrEMBL
Match: A0A5A7SY83 (Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002030 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.0e-193
Identity = 335/350 (95.71%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Carg26503 vs. ExPASy TrEMBL
Match: A0A1S3CLB4 (bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103501743 PE=3 SV=1)

HSP 1 Score: 683.7 bits (1763), Expect = 4.0e-193
Identity = 335/350 (95.71%), Postives = 341/350 (97.43%), Query Frame = 0


Query: 1   MALVPTTAATDTQLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60
           MALVPTTA TD QLIAEVDMG+D SV TVRATVVQASTIFYDTPATLDKAERLLAEAAGF
Sbjct: 1   MALVPTTAPTDPQLIAEVDMGTDSSVMTVRATVVQASTIFYDTPATLDKAERLLAEAAGF 60

Query: 61  GSQLVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120
           GSQLV+FPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK
Sbjct: 61  GSQLVVFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGK 120

Query: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180
           YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET
Sbjct: 121 YRVHLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFET 180

Query: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240
           SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN
Sbjct: 181 SIGKIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSAN 240

Query: 241 QFCRRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLD 300
           QFCRRKDYPPPP+YVFSGTEEEL+PDSVVCAGGS IISPSGTILAGPNYDGEALISADLD
Sbjct: 241 QFCRRKDYPPPPEYVFSGTEEELTPDSVVCAGGSAIISPSGTILAGPNYDGEALISADLD 300

Query: 301 LGDIARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDSCKK 351
           LG+IARAKFDFDVVGHYARPEVLSLVVRDHP  PVTFTS +TK EDSCKK
Sbjct: 301 LGEIARAKFDFDVVGHYARPEVLSLVVRDHPAIPVTFTSTSTKAEDSCKK 350

[Close]

BLAST of Carg26503 vs. TAIR 10
Match: AT5G22300.1 (nitrilase 4 )

HSP 1 Score: 548.9 bits (1413), Expect = 3.0e-156
Identity = 266/344 (77.33%), Postives = 302/344 (87.79%), Query Frame = 0


Query: 7   TAATDT---QLIAEVDMGSDVSVPTVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQ 66
           TAA  T   Q+  E+DM +  S   VRATVVQAST+FYDTPATLDKAERLL+EAA  GSQ
Sbjct: 11  TAAPQTNGHQIFPEIDMSAGDSSSIVRATVVQASTVFYDTPATLDKAERLLSEAAENGSQ 70

Query: 67  LVLFPEAFVGGYPRGSNFGVSIGNRTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRV 126
           LV+FPEAF+GGYPRGS F ++IG+RT KG+++FRKYHA+AIDVPGPEV+RLA MA KY+V
Sbjct: 71  LVVFPEAFIGGYPRGSTFELAIGSRTAKGRDDFRKYHASAIDVPGPEVERLALMAKKYKV 130

Query: 127 HLVMGVIERDGYTLYCTVLFFDPQGRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIG 186
           +LVMGVIER+GYTLYCTVLFFD QG +LGKHRK+MPTALER IWGFGDGSTIPVF+T IG
Sbjct: 131 YLVMGVIEREGYTLYCTVLFFDSQGLFLGKHRKLMPTALERCIWGFGDGSTIPVFDTPIG 190

Query: 187 KIGAAICWENRMPLLRTAMYAKGIEIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFC 246
           KIGAAICWENRMP LRTAMYAKGIEIYCAPTADSR+TW ASMTHIALEGGCFVLSANQFC
Sbjct: 191 KIGAAICWENRMPSLRTAMYAKGIEIYCAPTADSRETWLASMTHIALEGGCFVLSANQFC 250

Query: 247 RRKDYPPPPDYVFSGTEEELSPDSVVCAGGSVIISPSGTILAGPNYDGEALISADLDLGD 306
           RRKDYP PP+Y+FSG+EE L+PDSVVCAGGS IISP G +LAGPNY GEALI+ADLDLGD
Sbjct: 251 RRKDYPSPPEYMFSGSEESLTPDSVVCAGGSSIISPLGIVLAGPNYRGEALITADLDLGD 310

Query: 307 IARAKFDFDVVGHYARPEVLSLVVRDHPTTPVTFTSATTKTEDS 348
           IARAKFDFDVVGHY+RPEV SL +R+HP   V+F ++    ++S
Sbjct: 311 IARAKFDFDVVGHYSRPEVFSLNIREHPRKAVSFKTSKVMEDES 354

[Close]

BLAST of Carg26503 vs. TAIR 10
Match: AT3G44300.1 (nitrilase 2 )

HSP 1 Score: 471.1 bits (1211), Expect = 7.8e-133
Identity = 217/322 (67.39%), Postives = 262/322 (81.37%), Query Frame = 0


Query: 29  VRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGNR 88
           VRAT+VQAST++ DTPATL+KA + + EAA  GS+LV+FPEAF+GGYPRG  FG+ +G  
Sbjct: 18  VRATIVQASTVYNDTPATLEKANKFIVEAASKGSELVVFPEAFIGGYPRGFRFGLGVGVH 77

Query: 89  TPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQG 148
             +G++EFRKYHA+AI VPGPEV++LA +AGK  V+LVMG IE+DGYTLYCT LFF PQG
Sbjct: 78  NEEGRDEFRKYHASAIKVPGPEVEKLAELAGKNNVYLVMGAIEKDGYTLYCTALFFSPQG 137

Query: 149 RYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGIE 208
           ++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGIE
Sbjct: 138 QFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGIE 197

Query: 209 IYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDSV 268
           +YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC RKD+P  PDY+F+   ++  PDS+
Sbjct: 198 LYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCLRKDFPDHPDYLFTDWYDDKEPDSI 257

Query: 269 VCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVVR 328
           V  GGSVIISP G +LAGPN++ E LI+ADLDLGD+ARAK  FD VGHY+RP+VL L V 
Sbjct: 258 VSQGGSVIISPLGQVLAGPNFESEGLITADLDLGDVARAKLYFDSVGHYSRPDVLHLTVN 317

Query: 329 DHPTTPVTFTSATTKTEDSCKK 351
           +HP  PVTF S   K ED   K
Sbjct: 318 EHPKKPVTFISKVEKAEDDSNK 339

[Close]

BLAST of Carg26503 vs. TAIR 10
Match: AT3G44320.1 (nitrilase 3 )

HSP 1 Score: 470.7 bits (1210), Expect = 1.0e-132
Identity = 217/323 (67.18%), Postives = 262/323 (81.11%), Query Frame = 0


Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPATLDKAE+ + EAA  G++LVLFPEAF+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATLDKAEKFIVEAASKGAKLVLFPEAFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFR YHA+AI VPGPEV+RLA +AGK  VHLVMG IE+DGYTLYCT LFF PQ
Sbjct: 84  HNEEGRDEFRNYHASAIKVPGPEVERLAELAGKNNVHLVMGAIEKDGYTLYCTALFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRKVMPT+LER IWG GDGSTIPV++T IGKIGAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKVMPTSLERCIWGQGDGSTIPVYDTPIGKIGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDS 267
           EIYCAPTAD    WQASM HIA+EGGCFVLSA+QFC+R+++P  PDY+F+   +    D 
Sbjct: 204 EIYCAPTADYSLEWQASMIHIAVEGGCFVLSAHQFCKRREFPEHPDYLFNDIVDTKEHDP 263

Query: 268 VVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 327
            V  GGSVIISP G +LAGPNY+ E L++ADLDLGDIARAK  FDVVGHY++P++ +L V
Sbjct: 264 TVSGGGSVIISPLGKVLAGPNYESEGLVTADLDLGDIARAKLYFDVVGHYSKPDIFNLTV 323

Query: 328 RDHPTTPVTFTSATTKTEDSCKK 351
            +HP  PVTF +   K ED   K
Sbjct: 324 NEHPKKPVTFMTKVEKAEDESNK 346

[Close]

BLAST of Carg26503 vs. TAIR 10
Match: AT3G44310.1 (nitrilase 1 )

HSP 1 Score: 457.6 bits (1176), Expect = 9.0e-129
Identity = 211/323 (65.33%), Postives = 260/323 (80.50%), Query Frame = 0


Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LVLFPE F+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDS 267
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  PDY+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 268 VVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 327
           +V  GGSVIISP G +LAGPN++ E L++AD+DLGDIARAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 328 RDHPTTPVTFTSATTKTEDSCKK 351
            +HP   VTF +   K ED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

[Close]

BLAST of Carg26503 vs. TAIR 10
Match: AT3G44310.3 (nitrilase 1 )

HSP 1 Score: 457.6 bits (1176), Expect = 9.0e-129
Identity = 211/323 (65.33%), Postives = 260/323 (80.50%), Query Frame = 0


Query: 28  TVRATVVQASTIFYDTPATLDKAERLLAEAAGFGSQLVLFPEAFVGGYPRGSNFGVSIGN 87
           TVR T+VQ+ST++ DTPAT+DKAE+ + EAA  G++LVLFPE F+GGYPRG  FG+++G 
Sbjct: 24  TVRVTIVQSSTVYNDTPATIDKAEKYIVEAASKGAELVLFPEGFIGGYPRGFRFGLAVGV 83

Query: 88  RTPKGKEEFRKYHAAAIDVPGPEVDRLAAMAGKYRVHLVMGVIERDGYTLYCTVLFFDPQ 147
              +G++EFRKYHA+AI VPGPEV RLA +A K  V+LVMG IE++GYTLYCTVLFF PQ
Sbjct: 84  HNEEGRDEFRKYHASAIHVPGPEVARLADVARKNHVYLVMGAIEKEGYTLYCTVLFFSPQ 143

Query: 148 GRYLGKHRKVMPTALERIIWGFGDGSTIPVFETSIGKIGAAICWENRMPLLRTAMYAKGI 207
           G++LGKHRK+MPT+LER IWG GDGSTIPV++T IGK+GAAICWENRMPL RTA+YAKGI
Sbjct: 144 GQFLGKHRKLMPTSLERCIWGQGDGSTIPVYDTPIGKLGAAICWENRMPLYRTALYAKGI 203

Query: 208 EIYCAPTADSRDTWQASMTHIALEGGCFVLSANQFCRRKDYPPPPDYVFSGTEEELSPDS 267
           E+YCAPTAD    WQ+SM HIA+EGGCFVLSA QFC+RK +P  PDY+F+   ++   DS
Sbjct: 204 ELYCAPTADGSKEWQSSMLHIAIEGGCFVLSACQFCQRKHFPDHPDYLFTDWYDDKEHDS 263

Query: 268 VVCAGGSVIISPSGTILAGPNYDGEALISADLDLGDIARAKFDFDVVGHYARPEVLSLVV 327
           +V  GGSVIISP G +LAGPN++ E L++AD+DLGDIARAK  FD VGHY+RP+VL L V
Sbjct: 264 IVSQGGSVIISPLGQVLAGPNFESEGLVTADIDLGDIARAKLYFDSVGHYSRPDVLHLTV 323

Query: 328 RDHPTTPVTFTSATTKTEDSCKK 351
            +HP   VTF +   K ED   K
Sbjct: 324 NEHPRKSVTFVTKVEKAEDDSNK 346

The following BLAST results are available for this feature:

BLAST of Carg26503 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583706.1	4.8e-201	100.00	Bifunctional nitrilase/nitrile hydratase NIT4A, partial [Cucurbita argyrosperma ...	[more]
XP_023520715.1	3.1e-200	99.43	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita pepo subsp. pepo]	[more]
XP_022927562.1	5.9e-199	99.14	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita moschata]	[more]
XP_022973146.1	2.2e-198	98.57	bifunctional nitrilase/nitrile hydratase NIT4A [Cucurbita maxima]	[more]
XP_004139500.1	9.8e-194	96.00	bifunctional nitrilase/nitrile hydratase NIT4A [Cucumis sativus] >KGN64959.1 hyp...	[more]

BLAST of Carg26503 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q5QGZ8	7.8e-178	85.67	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Lupinus angustifolius OX=3871 ...	[more]
Q42965	8.6e-177	85.67	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q42966	3.3e-176	85.39	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Nicotiana tabacum OX=4097 GN=N...	[more]
Q3LRV4	1.4e-174	85.14	Bifunctional nitrilase/nitrile hydratase NIT4B OS=Lupinus angustifolius OX=3871 ...	[more]
Q6H849	1.6e-162	80.29	Bifunctional nitrilase/nitrile hydratase NIT4 OS=Oryza sativa subsp. japonica OX...	[more]

BLAST of Carg26503 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EHI6	2.9e-199	99.14	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1I7W0	1.1e-198	98.57	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucurbita maxima OX=3661 GN=LO...	[more]
A0A0A0LVI0	4.7e-194	96.00	CN hydrolase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G16836...	[more]
A0A5A7SY83	4.0e-193	95.71	Bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3CLB4	4.0e-193	95.71	bifunctional nitrilase/nitrile hydratase NIT4A OS=Cucumis melo OX=3656 GN=LOC103...	[more]

BLAST of Carg26503 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G22300.1	3.0e-156	77.33	nitrilase 4	[more]
AT3G44300.1	7.8e-133	67.39	nitrilase 2	[more]
AT3G44320.1	1.0e-132	67.18	nitrilase 3	[more]
AT3G44310.1	9.0e-129	65.33	nitrilase 1	[more]
AT3G44310.3	9.0e-129	65.33	nitrilase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003010	Carbon-nitrogen hydrolase	PFAM	PF00795	CN_hydrolase	coord: 30..308 e-value: 1.1E-68 score: 231.4
IPR003010	Carbon-nitrogen hydrolase	PROSITE	PS50263	CN_HYDROLASE	coord: 29..301 score: 57.435909
IPR036526	Carbon-nitrogen hydrolase superfamily	GENE3D	3.60.110.10		coord: 22..313 e-value: 2.1E-85 score: 288.2
IPR036526	Carbon-nitrogen hydrolase superfamily	SUPERFAMILY	56317	Carbon-nitrogen hydrolase	coord: 27..327
None	No IPR available	PANTHER	PTHR46044:SF1	NITRILASE	coord: 13..344
IPR044149	Nitrilase/Cyanide hydratase	PANTHER	PTHR46044	NITRILASE	coord: 13..344
IPR044149	Nitrilase/Cyanide hydratase	CDD	cd07564	nitrilases_CHs	coord: 29..329 e-value: 1.14943E-161 score: 451.942
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00921	NITRIL_CHT_2	coord: 186..199
IPR000132	Nitrilase/cyanide hydratase, conserved site	PROSITE	PS00920	NITRIL_CHT_1	coord: 61..76

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26503-RA	Carg26503-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051410	detoxification of nitrogen compound
biological_process	GO:0006807	nitrogen compound metabolic process
molecular_function	GO:0047427	cyanoalanine nitrilase activity
molecular_function	GO:0018822	nitrile hydratase activity
molecular_function	GO:0003824	catalytic activity