Carg26471 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26471
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionBifunctional dihydrofolate reductase-thymidylate synthase
LocationCarg_Chr20: 4224537 .. 4226531 (+)
RNA-Seq ExpressionCarg26471
SyntenyCarg26471
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAAGGTAAGGCGCTATGCTATCACTAGCACTTAAAGCTTTACTTCACTAGTTTGTGGTTGTGATTGTACAACCCCGCACCCCGCACCCCGCACGTTGAGTTAGAATTTCTTGGTTTAGGAATTAGGAACTAAGCGATTAAGTGTTTTGCTTTCTTGGTTTAGTGGTTGTGGTGTTTCGGTTCATAGCTTGGGGCCTTGTATGATTTGTGTTTCGGTTCACAGTCTCCCTAGTGTTCCTCTGTGAAATCCCACGTCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCGCTAGTACATGCGTTTTAAAAACCTTGAGGGGAAGCCTGGAAGGGAAAGCCCAAAGATGACGATATCTGCTAGCGGTGGGCTTGGGCTTGAGCTGTTCTCAACGTAGGGCTAGACCTACTCCATTGTGGTTTATCTTGCATTGGCATCTTGTTTTGGCCTACAAGTGAGGCCTTATTTTCGACATTATCAACAGGTTCTTCAGGAGAAAGGCATTCATATATGGGATGGCAACGCGTCGAGAGATTACCTCGATAGGCAATTACTTATACCTTCGAAATTTGATTCTTTTTAATATGAAACTAGAGAAGTTTGAGTTTTTTTTGTATATAATTTGAGCTATAATTCTGTTTTTTTTTTGGGTAATCTTAGTATTGGTTTAAGAGAAAGGGAGGAAGGCGACTTGGGACCCGTATACGGGTTTCAGTGGCGACACTTTGGTGCTAGGTTCGTCGATAACTTCGATGATCTCTTAAGTTTGTTTTTATTTTATTGCGGATTCGAGCTTTAATGCTTTCGACGCTTCTTCTACAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGGTTGGACTCAAGGGCATGATGTTCCGTGTCTTTAACTTGTTTCGTTAATGTTGTTTATTGATCGCCTTTTGCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGGTGTTCCATTTAATATTGCGTCGTATGCTCTTCTGACGTGCATGATTGCTCATGTTTGTGGTATGCTACTCTATATTGATCAGTAGTTTGTTTTCTACTATTGTGAGATCCCACATCGATTGGAGAGGGGAACGAATCATTCTTTATAAGGGTGTAGAAACCTCTCCGTAATAAACACGTTTTAAAACCGTGAGGCTGACGACGATACGTAATGGGTCAAAGTGGACAATATCTGTTAGTAGTAGGCTTGGGTTGTTACAAATGGTATCAGAACTAGACACCGGGCAGTGTGCCAGCGAGGTCGCTGGGCTCCCAAGGGGGTGGATAGTGAGATCCCACATCGGTTGGAGACGGGAACGAAGCATTGTTTATAAGGGTGTGGAAACCTCTCCCTAACAAACGCGTTTTAAAACCGTGAGGCTGATGGCAATACGTAACTAGACAACCGGACGATTATACACATCTATATGGCTCTTTAAACATTCCGAACCTACATTTATACTTTTAGTTACATAAAAAAGTTTATTCTTCCTACGTGCAGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTAAGTCGGAAAACTATAATTACATGGTTCTTCCATGCTCGCGTTCACTAACTTGCTGTTTTTAATACTACACCGGCCTTACCTTATTATAGGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

mRNA sequence

ATGCAAAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

Coding sequence (CDS)

ATGCAAAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

Protein sequence

MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Homology
[Close]
BLAST of Carg26471 vs. NCBI nr
Match: KAG7010921.1 (Bifunctional dihydrofolate reductase-thymidylate synthase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.9 bits (816), Expect = 2.3e-83
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0 

Query: 1   MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY 60
           MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY
Sbjct: 1   MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY 60

Query: 61  VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN 120
           VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN
Sbjct: 61  VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN 120

Query: 121 PEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           PEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 121 PEKKDIDSFVAADFELTGYDPHEKIAMKMAV 151

[Close]
BLAST of Carg26471 vs. NCBI nr
Match: XP_022944443.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata] >XP_022944444.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata])

HSP 1 Score: 303.1 bits (775), Expect = 1.3e-78
Identity = 149/169 (88.17%), Postives = 149/169 (88.17%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 528

[Close]
BLAST of Carg26471 vs. NCBI nr
Match: XP_022986497.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 5.0e-78
Identity = 148/169 (87.57%), Postives = 149/169 (88.17%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 357 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 416

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 417 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 476

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 477 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 525

[Close]
BLAST of Carg26471 vs. NCBI nr
Match: XP_023513067.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo] >XP_023513068.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.8 bits (769), Expect = 6.5e-78
Identity = 148/169 (87.57%), Postives = 148/169 (87.57%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDS VAADFELTGYDPHEKIAMKMAV
Sbjct: 481 LQEQLQKLPKPFPVLKINPEKKDIDSLVAADFELTGYDPHEKIAMKMAV 529

[Close]
BLAST of Carg26471 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 292.7 bits (748), Expect = 1.8e-75
Identity = 143/169 (84.62%), Postives = 146/169 (86.39%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFV+ DFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPILKINPEKKDIDSFVSDDFELTGYDPHEKIAMKMAV 528

[Close]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q05763 (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 5.9e-74
Identity = 135/169 (79.88%), Postives = 141/169 (83.43%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 397 KYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 456

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DL  GDFIHVIGDAHVY+ HVRP
Sbjct: 457 NGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRP 516

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL+  PKPFPVLKINPEKKDIDSFVA DFEL GYDPH+KI MKMAV
Sbjct: 517 LQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

[Close]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 274.6 bits (701), Expect = 6.5e-73
Identity = 131/169 (77.51%), Postives = 142/169 (84.02%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 351 KYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 410

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
            GELSCQMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRP
Sbjct: 411 EGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRP 470

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL  LPKPFPV+KINPEKK IDSFVA+DF+LTGYDPH+KI MKMAV
Sbjct: 471 LQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

[Close]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 4.0e-70
Identity = 128/169 (75.74%), Postives = 137/169 (81.07%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMH DY+GQGFDQLLDVINKIK NPDDRRI+LSAWNP DLKLMALPPCHMFAQFYVA
Sbjct: 362 RYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMALPPCHMFAQFYVA 421

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           +GELSCQMYQRSADMGL                    DL  GDFIHVIGDAH+YR HVRP
Sbjct: 422 HGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHVRP 481

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL   PKPFP LKINP+KKDIDSFVAADF+L GYDPH+KI MK++V
Sbjct: 482 LQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIGYDPHQKIDMKLSV 530

[Close]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 8.8e-70
Identity = 124/168 (73.81%), Postives = 140/168 (83.33%), Query Frame = 0 

Query: 4   YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVAN 63
           YTDMHADYTG+GFDQL+DVI+KIKN+P+DRRI+LSAWNPSDLK MALPPCHMFAQFYV N
Sbjct: 354 YTDMHADYTGKGFDQLMDVIDKIKNDPEDRRIILSAWNPSDLKKMALPPCHMFAQFYVEN 413

Query: 64  GELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPL 123
           GELSCQMYQRSADMGL                    DL+ GDF+HVIGDAHVYR HVR L
Sbjct: 414 GELSCQMYQRSADMGLGVPFNIASYSLLTYMIAQVCDLSPGDFVHVIGDAHVYRNHVRAL 473

Query: 124 QEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           +EQ+QK+PKPFP+LKINP KKDIDSF+A+DF+L GYDPH+KI MKMAV
Sbjct: 474 EEQIQKMPKPFPILKINPSKKDIDSFMASDFKLVGYDPHQKIEMKMAV 521

[Close]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q2QRX6 (Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0446900 PE=3 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 1.8e-67
Identity = 122/168 (72.62%), Postives = 135/168 (80.36%), Query Frame = 0 

Query: 4   YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVAN 63
           YTDMHADY G+GFDQL+DVI+KIKNNPDDRRI+LSAWNP+DLK MALPPCHMFAQFYV N
Sbjct: 327 YTDMHADYVGKGFDQLMDVIDKIKNNPDDRRIILSAWNPTDLKKMALPPCHMFAQFYVEN 386

Query: 64  GELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPL 123
           GELSCQMYQRSADMGL                    DL+ GDF+HVIGDAHVYRTHV  L
Sbjct: 387 GELSCQMYQRSADMGLGVPFNIASYSLLTCMIAQVCDLSPGDFVHVIGDAHVYRTHVEAL 446

Query: 124 QEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           +EQ++K PKPFP+LKINP KKDIDSFV +DF+L  YDPH KI MKMAV
Sbjct: 447 EEQMRKQPKPFPILKINPVKKDIDSFVTSDFKLVRYDPHHKIEMKMAV 494

[Close]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.3e-79
Identity = 149/169 (88.17%), Postives = 149/169 (88.17%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 528

[Close]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1J7P4 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111484218 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.4e-78
Identity = 148/169 (87.57%), Postives = 149/169 (88.17%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 357 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 416

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 417 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 476

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 477 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 525

[Close]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1CYC5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia OX=3673 GN=LOC111015400 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.6e-76
Identity = 142/169 (84.02%), Postives = 146/169 (86.39%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 369 RYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 428

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLA GDFIHVIGDAHVY THVRP
Sbjct: 429 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRP 488

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSF+AADFELTGYDPHEKIAMKMAV
Sbjct: 489 LQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYDPHEKIAMKMAV 537

[Close]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A1S3CE37 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=3656 GN=LOC103499691 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.6e-75
Identity = 142/169 (84.02%), Postives = 145/169 (85.80%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFVA DFELTGYDPH KIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 528

[Close]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A5D3DYK8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00260 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.6e-75
Identity = 142/169 (84.02%), Postives = 145/169 (85.80%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVA
Sbjct: 408 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVA 467

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 468 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 527

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFVA DFELTGYDPH KIAMKMAV
Sbjct: 528 LQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 576

[Close]
BLAST of Carg26471 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 278.1 bits (710), Expect = 4.2e-75
Identity = 135/169 (79.88%), Postives = 141/169 (83.43%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 397 KYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 456

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DL  GDFIHVIGDAHVY+ HVRP
Sbjct: 457 NGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRP 516

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL+  PKPFPVLKINPEKKDIDSFVA DFEL GYDPH+KI MKMAV
Sbjct: 517 LQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

[Close]
BLAST of Carg26471 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 274.6 bits (701), Expect = 4.6e-74
Identity = 131/169 (77.51%), Postives = 142/169 (84.02%), Query Frame = 0 

Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 351 KYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 410

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
            GELSCQMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRP
Sbjct: 411 EGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRP 470

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL  LPKPFPV+KINPEKK IDSFVA+DF+LTGYDPH+KI MKMAV
Sbjct: 471 LQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

[Close]
BLAST of Carg26471 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 155.2 bits (391), Expect = 4.1e-38
Identity = 79/148 (53.38%), Postives = 99/148 (66.89%), Query Frame = 0 

Query: 9   ADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSC 68
           A +  Q F QL DVINKIKNNP D+RI+L+A NP D KL ++ PCH F QFYVANGE+SC
Sbjct: 343 ARFVIQEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYVANGEVSC 402

Query: 69  QMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQ 128
           Q+YQ S +  +                    DL  GDFIHVIG A++ + HV+ +Q+QLQ
Sbjct: 403 QIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVKAIQKQLQ 462

Query: 129 KLPKPFPVLKINPEKKDIDSFVAADFEL 137
             PKPFP+LKINPEKK +D+F A+D EL
Sbjct: 463 ISPKPFPILKINPEKKKMDNFEASDLEL 489

The following BLAST results are available for this feature:
BLAST of Carg26471 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7010921.12.3e-83100.00Bifunctional dihydrofolate reductase-thymidylate synthase 2, partial [Cucurbita ... [more]
XP_022944443.11.3e-7888.17bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moscha... [more]
XP_022986497.15.0e-7887.57bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima... [more]
XP_023513067.16.5e-7887.57bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo s... [more]
XP_038901596.11.8e-7584.62bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp... [more]
BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q057635.9e-7479.88Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thali... [more]
Q057626.5e-7377.51Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali... [more]
P518204.0e-7075.74Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847... [more]
O813958.8e-7073.81Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN... [more]
Q2QRX61.8e-6772.62Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sati... [more]
BLAST of Carg26471 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FWP86.3e-7988.17Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ... [more]
A0A6J1J7P42.4e-7887.57Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX... [more]
A0A6J1CYC58.6e-7684.02Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia... [more]
A0A1S3CE375.6e-7584.02Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=365... [more]
A0A5D3DYK85.6e-7584.02Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m... [more]
BLAST of Carg26471 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G34570.14.2e-7579.88thymidylate synthase 2 [more]
AT2G16370.14.6e-7477.51thymidylate synthase 1 [more]
AT2G21550.14.1e-3853.38Bifunctional dihydrofolate reductase/thymidylate synthase [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000398Thymidylate synthasePRINTSPR00108THYMDSNTHASEcoord: 66..92
score: 41.77
coord: 22..41
score: 60.96
coord: 48..63
score: 73.54
IPR023451Thymidylate synthase/dCMP hydroxymethylase domainPFAMPF00303Thymidylat_syntcoord: 80..151
e-value: 1.6E-20
score: 73.4
coord: 7..82
e-value: 4.3E-29
score: 101.4
IPR023451Thymidylate synthase/dCMP hydroxymethylase domainCDDcd00351TS_Pyrimidine_HMasecoord: 8..104
e-value: 3.99108E-48
score: 152.815
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3D3.30.572.10Thymidylate synthase/dCMP hydroxymethylase domaincoord: 1..80
e-value: 8.0E-37
score: 128.6
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilyGENE3D3.30.572.10Thymidylate synthase/dCMP hydroxymethylase domaincoord: 81..151
e-value: 3.5E-25
score: 90.7
IPR036926Thymidylate synthase/dCMP hydroxymethylase superfamilySUPERFAMILY55831Thymidylate synthase/dCMP hydroxymethylasecoord: 3..151
IPR045097Thymidylate synthase/dCMP hydroxymethylasePANTHERPTHR11548THYMIDYLATE SYNTHASE 1coord: 3..81
IPR045097Thymidylate synthase/dCMP hydroxymethylasePANTHERPTHR11548THYMIDYLATE SYNTHASE 1coord: 83..151
NoneNo IPR availablePANTHERPTHR11548:SF14BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASEcoord: 3..81
NoneNo IPR availablePANTHERPTHR11548:SF14BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASEcoord: 83..151
IPR020940Thymidylate synthase, active sitePROSITEPS00091THYMIDYLATE_SYNTHASEcoord: 33..61

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26471-RACarg26471-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006231 dTMP biosynthetic process
biological_process GO:0032259 methylation
biological_process GO:0006730 one-carbon metabolic process
biological_process GO:0046654 tetrahydrofolate biosynthetic process
cellular_component GO:0005829 cytosol
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004146 dihydrofolate reductase activity
molecular_function GO:0004799 thymidylate synthase activity
molecular_function GO:0016741 transferase activity, transferring one-carbon groups