Carg26471 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26471
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Bifunctional dihydrofolate reductase-thymidylate synthase
Location	Carg_Chr20: 4224537 .. 4226531 (+)
RNA-Seq Expression	Carg26471
Synteny	Carg26471

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAGGTAAGGCGCTATGCTATCACTAGCACTTAAAGCTTTACTTCACTAGTTTGTGGTTGTGATTGTACAACCCCGCACCCCGCACCCCGCACGTTGAGTTAGAATTTCTTGGTTTAGGAATTAGGAACTAAGCGATTAAGTGTTTTGCTTTCTTGGTTTAGTGGTTGTGGTGTTTCGGTTCATAGCTTGGGGCCTTGTATGATTTGTGTTTCGGTTCACAGTCTCCCTAGTGTTCCTCTGTGAAATCCCACGTCGGTTGGGGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCGCTAGTACATGCGTTTTAAAAACCTTGAGGGGAAGCCTGGAAGGGAAAGCCCAAAGATGACGATATCTGCTAGCGGTGGGCTTGGGCTTGAGCTGTTCTCAACGTAGGGCTAGACCTACTCCATTGTGGTTTATCTTGCATTGGCATCTTGTTTTGGCCTACAAGTGAGGCCTTATTTTCGACATTATCAACAGGTTCTTCAGGAGAAAGGCATTCATATATGGGATGGCAACGCGTCGAGAGATTACCTCGATAGGCAATTACTTATACCTTCGAAATTTGATTCTTTTTAATATGAAACTAGAGAAGTTTGAGTTTTTTTTGTATATAATTTGAGCTATAATTCTGTTTTTTTTTTGGGTAATCTTAGTATTGGTTTAAGAGAAAGGGAGGAAGGCGACTTGGGACCCGTATACGGGTTTCAGTGGCGACACTTTGGTGCTAGGTTCGTCGATAACTTCGATGATCTCTTAAGTTTGTTTTTATTTTATTGCGGATTCGAGCTTTAATGCTTTCGACGCTTCTTCTACAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGGTTGGACTCAAGGGCATGATGTTCCGTGTCTTTAACTTGTTTCGTTAATGTTGTTTATTGATCGCCTTTTGCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGGTGTTCCATTTAATATTGCGTCGTATGCTCTTCTGACGTGCATGATTGCTCATGTTTGTGGTATGCTACTCTATATTGATCAGTAGTTTGTTTTCTACTATTGTGAGATCCCACATCGATTGGAGAGGGGAACGAATCATTCTTTATAAGGGTGTAGAAACCTCTCCGTAATAAACACGTTTTAAAACCGTGAGGCTGACGACGATACGTAATGGGTCAAAGTGGACAATATCTGTTAGTAGTAGGCTTGGGTTGTTACAAATGGTATCAGAACTAGACACCGGGCAGTGTGCCAGCGAGGTCGCTGGGCTCCCAAGGGGGTGGATAGTGAGATCCCACATCGGTTGGAGACGGGAACGAAGCATTGTTTATAAGGGTGTGGAAACCTCTCCCTAACAAACGCGTTTTAAAACCGTGAGGCTGATGGCAATACGTAACTAGACAACCGGACGATTATACACATCTATATGGCTCTTTAAACATTCCGAACCTACATTTATACTTTTAGTTACATAAAAAAGTTTATTCTTCCTACGTGCAGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTAAGTCGGAAAACTATAATTACATGGTTCTTCCATGCTCGCGTTCACTAACTTGCTGTTTTTAATACTACACCGGCCTTACCTTATTATAGGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

mRNA sequence

ATGCAAAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

Coding sequence (CDS)

ATGCAAAGGTATACTGACATGCATGCTGACTACACTGGTCAAGGATTTGATCAGTTGCTAGACGTTATTAACAAGATAAAAAATAATCCCGATGATCGGAGGATCGTTCTTTCTGCGTGGAATCCATCTGATCTCAAACTGATGGCACTCCCGCCTTGCCACATGTTCGCTCAGTTTTATGTAGCGAACGGGGAGTTATCGTGTCAAATGTATCAGCGCTCTGCTGATATGGGCCTTGATCTTGCTTACGGAGACTTCATACATGTGATCGGAGACGCTCATGTTTACCGTACTCACGTGCGGCCATTGCAGGAACAGCTTCAGAAACTCCCGAAACCGTTCCCTGTTTTGAAGATAAACCCCGAGAAGAAGGATATCGATTCGTTCGTGGCAGCTGATTTCGAGCTAACAGGTTATGACCCTCATGAGAAGATAGCAATGAAAATGGCAGTATAG

Protein sequence

MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV

Homology

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BLAST of Carg26471 vs. NCBI nr
Match: KAG7010921.1 (Bifunctional dihydrofolate reductase-thymidylate synthase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 318.9 bits (816), Expect = 2.3e-83
Identity = 151/151 (100.00%), Postives = 151/151 (100.00%), Query Frame = 0


Query: 1   MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY 60
           MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY
Sbjct: 1   MQRYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFY 60

Query: 61  VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN 120
           VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN
Sbjct: 61  VANGELSCQMYQRSADMGLDLAYGDFIHVIGDAHVYRTHVRPLQEQLQKLPKPFPVLKIN 120

Query: 121 PEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           PEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 121 PEKKDIDSFVAADFELTGYDPHEKIAMKMAV 151

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BLAST of Carg26471 vs. NCBI nr
Match: XP_022944443.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata] >XP_022944444.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moschata])

HSP 1 Score: 303.1 bits (775), Expect = 1.3e-78
Identity = 149/169 (88.17%), Postives = 149/169 (88.17%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 528

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BLAST of Carg26471 vs. NCBI nr
Match: XP_022986497.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima])

HSP 1 Score: 301.2 bits (770), Expect = 5.0e-78
Identity = 148/169 (87.57%), Postives = 149/169 (88.17%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 357 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 416

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 417 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 476

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 477 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 525

[Close]

BLAST of Carg26471 vs. NCBI nr
Match: XP_023513067.1 (bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo] >XP_023513068.1 bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 300.8 bits (769), Expect = 6.5e-78
Identity = 148/169 (87.57%), Postives = 148/169 (87.57%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 361 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 420

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 421 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 480

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDS VAADFELTGYDPHEKIAMKMAV
Sbjct: 481 LQEQLQKLPKPFPVLKINPEKKDIDSLVAADFELTGYDPHEKIAMKMAV 529

[Close]

BLAST of Carg26471 vs. NCBI nr
Match: XP_038901596.1 (bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida] >XP_038901597.1 bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hispida])

HSP 1 Score: 292.7 bits (748), Expect = 1.8e-75
Identity = 143/169 (84.62%), Postives = 146/169 (86.39%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFV+ DFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPILKINPEKKDIDSFVSDDFELTGYDPHEKIAMKMAV 528

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BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q05763 (Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thaliana OX=3702 GN=THY-2 PE=2 SV=2)

HSP 1 Score: 278.1 bits (710), Expect = 5.9e-74
Identity = 135/169 (79.88%), Postives = 141/169 (83.43%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 397 KYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 456

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DL  GDFIHVIGDAHVY+ HVRP
Sbjct: 457 NGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRP 516

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL+  PKPFPVLKINPEKKDIDSFVA DFEL GYDPH+KI MKMAV
Sbjct: 517 LQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

[Close]

BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q05762 (Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thaliana OX=3702 GN=THY-1 PE=1 SV=2)

HSP 1 Score: 274.6 bits (701), Expect = 6.5e-73
Identity = 131/169 (77.51%), Postives = 142/169 (84.02%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 351 KYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 410

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
            GELSCQMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRP
Sbjct: 411 EGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRP 470

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL  LPKPFPV+KINPEKK IDSFVA+DF+LTGYDPH+KI MKMAV
Sbjct: 471 LQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

[Close]

BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: P51820 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 4.0e-70
Identity = 128/169 (75.74%), Postives = 137/169 (81.07%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMH DY+GQGFDQLLDVINKIK NPDDRRI+LSAWNP DLKLMALPPCHMFAQFYVA
Sbjct: 362 RYTDMHHDYSGQGFDQLLDVINKIKRNPDDRRIILSAWNPVDLKLMALPPCHMFAQFYVA 421

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           +GELSCQMYQRSADMGL                    DL  GDFIHVIGDAH+YR HVRP
Sbjct: 422 HGELSCQMYQRSADMGLGIPFNIASYALLTCMIAHVCDLIPGDFIHVIGDAHIYRNHVRP 481

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL   PKPFP LKINP+KKDIDSFVAADF+L GYDPH+KI MK++V
Sbjct: 482 LQEQLHNQPKPFPTLKINPKKKDIDSFVAADFKLIGYDPHQKIDMKLSV 530

[Close]

BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: O81395 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN=DRTS PE=2 SV=1)

HSP 1 Score: 264.2 bits (674), Expect = 8.8e-70
Identity = 124/168 (73.81%), Postives = 140/168 (83.33%), Query Frame = 0


Query: 4   YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVAN 63
           YTDMHADYTG+GFDQL+DVI+KIKN+P+DRRI+LSAWNPSDLK MALPPCHMFAQFYV N
Sbjct: 354 YTDMHADYTGKGFDQLMDVIDKIKNDPEDRRIILSAWNPSDLKKMALPPCHMFAQFYVEN 413

Query: 64  GELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPL 123
           GELSCQMYQRSADMGL                    DL+ GDF+HVIGDAHVYR HVR L
Sbjct: 414 GELSCQMYQRSADMGLGVPFNIASYSLLTYMIAQVCDLSPGDFVHVIGDAHVYRNHVRAL 473

Query: 124 QEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           +EQ+QK+PKPFP+LKINP KKDIDSF+A+DF+L GYDPH+KI MKMAV
Sbjct: 474 EEQIQKMPKPFPILKINPSKKDIDSFMASDFKLVGYDPHQKIEMKMAV 521

[Close]

BLAST of Carg26471 vs. ExPASy Swiss-Prot
Match: Q2QRX6 (Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os12g0446900 PE=3 SV=2)

HSP 1 Score: 256.5 bits (654), Expect = 1.8e-67
Identity = 122/168 (72.62%), Postives = 135/168 (80.36%), Query Frame = 0


Query: 4   YTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVAN 63
           YTDMHADY G+GFDQL+DVI+KIKNNPDDRRI+LSAWNP+DLK MALPPCHMFAQFYV N
Sbjct: 327 YTDMHADYVGKGFDQLMDVIDKIKNNPDDRRIILSAWNPTDLKKMALPPCHMFAQFYVEN 386

Query: 64  GELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPL 123
           GELSCQMYQRSADMGL                    DL+ GDF+HVIGDAHVYRTHV  L
Sbjct: 387 GELSCQMYQRSADMGLGVPFNIASYSLLTCMIAQVCDLSPGDFVHVIGDAHVYRTHVEAL 446

Query: 124 QEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           +EQ++K PKPFP+LKINP KKDIDSFV +DF+L  YDPH KI MKMAV
Sbjct: 447 EEQMRKQPKPFPILKINPVKKDIDSFVTSDFKLVRYDPHHKIEMKMAV 494

[Close]

BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1FWP8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata OX=3662 GN=LOC111448895 PE=3 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.3e-79
Identity = 149/169 (88.17%), Postives = 149/169 (88.17%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 528

[Close]

BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1J7P4 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX=3661 GN=LOC111484218 PE=3 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 2.4e-78
Identity = 148/169 (87.57%), Postives = 149/169 (88.17%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNP+DRRIVLSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 357 RYTDMHADYTGQGFDQLLDVINKIKNNPNDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 416

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLAYGDFIHVIGDAHVYRTHVRP
Sbjct: 417 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAYGDFIHVIGDAHVYRTHVRP 476

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV
Sbjct: 477 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 525

[Close]

BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A6J1CYC5 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia OX=3673 GN=LOC111015400 PE=3 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.6e-76
Identity = 142/169 (84.02%), Postives = 146/169 (86.39%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIK NP+DRRI+LSAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 369 RYTDMHADYTGQGFDQLLDVINKIKTNPNDRRIILSAWNPSDLKLMALPPCHMFAQFYVA 428

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DLA GDFIHVIGDAHVY THVRP
Sbjct: 429 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCDLAQGDFIHVIGDAHVYHTHVRP 488

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSF+AADFELTGYDPHEKIAMKMAV
Sbjct: 489 LQEQLQKLPKPFPILKINPEKKDIDSFIAADFELTGYDPHEKIAMKMAV 537

[Close]

BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A1S3CE37 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=3656 GN=LOC103499691 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.6e-75
Identity = 142/169 (84.02%), Postives = 145/169 (85.80%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVA
Sbjct: 360 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVA 419

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 420 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 479

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFVA DFELTGYDPH KIAMKMAV
Sbjct: 480 LQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 528

[Close]

BLAST of Carg26471 vs. ExPASy TrEMBL
Match: A0A5D3DYK8 (Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00260 PE=3 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 5.6e-75
Identity = 142/169 (84.02%), Postives = 145/169 (85.80%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRI+LSAWNPSDLKLMALPPCHMF+QFYVA
Sbjct: 408 RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIILSAWNPSDLKLMALPPCHMFSQFYVA 467

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                     LA GDFIHVIGDAHVYRTHVRP
Sbjct: 468 NGELSCQMYQRSADMGLGVPFNIASYALLTCMIAHVCGLARGDFIHVIGDAHVYRTHVRP 527

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQLQKLPKPFP+LKINPEKKDIDSFVA DFELTGYDPH KIAMKMAV
Sbjct: 528 LQEQLQKLPKPFPILKINPEKKDIDSFVADDFELTGYDPHVKIAMKMAV 576

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BLAST of Carg26471 vs. TAIR 10
Match: AT4G34570.1 (thymidylate synthase 2 )

HSP 1 Score: 278.1 bits (710), Expect = 4.2e-75
Identity = 135/169 (79.88%), Postives = 141/169 (83.43%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQLLDVINKIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 397 KYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 456

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
           NGELSCQMYQRSADMGL                    DL  GDFIHVIGDAHVY+ HVRP
Sbjct: 457 NGELSCQMYQRSADMGLGVPFNIASYSLLTCILAHVCDLVPGDFIHVIGDAHVYKNHVRP 516

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL+  PKPFPVLKINPEKKDIDSFVA DFEL GYDPH+KI MKMAV
Sbjct: 517 LQEQLENPPKPFPVLKINPEKKDIDSFVADDFELIGYDPHKKIDMKMAV 565

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BLAST of Carg26471 vs. TAIR 10
Match: AT2G16370.1 (thymidylate synthase 1 )

HSP 1 Score: 274.6 bits (701), Expect = 4.6e-74
Identity = 131/169 (77.51%), Postives = 142/169 (84.02%), Query Frame = 0


Query: 3   RYTDMHADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVA 62
           +YTDMHADYTGQGFDQL+DVI+KIKNNPDDRRI++SAWNPSDLKLMALPPCHMFAQFYVA
Sbjct: 351 KYTDMHADYTGQGFDQLVDVIDKIKNNPDDRRIIMSAWNPSDLKLMALPPCHMFAQFYVA 410

Query: 63  NGELSCQMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRP 122
            GELSCQMYQRSADMGL                    DL  GDFIHV+GDAHVY+THVRP
Sbjct: 411 EGELSCQMYQRSADMGLGVPFNIASYSLLTCMLAHVCDLVPGDFIHVLGDAHVYKTHVRP 470

Query: 123 LQEQLQKLPKPFPVLKINPEKKDIDSFVAADFELTGYDPHEKIAMKMAV 152
           LQEQL  LPKPFPV+KINPEKK IDSFVA+DF+LTGYDPH+KI MKMAV
Sbjct: 471 LQEQLLNLPKPFPVMKINPEKKQIDSFVASDFDLTGYDPHKKIEMKMAV 519

[Close]

BLAST of Carg26471 vs. TAIR 10
Match: AT2G21550.1 (Bifunctional dihydrofolate reductase/thymidylate synthase )

HSP 1 Score: 155.2 bits (391), Expect = 4.1e-38
Identity = 79/148 (53.38%), Postives = 99/148 (66.89%), Query Frame = 0


Query: 9   ADYTGQGFDQLLDVINKIKNNPDDRRIVLSAWNPSDLKLMALPPCHMFAQFYVANGELSC 68
           A +  Q F QL DVINKIKNNP D+RI+L+A NP D KL ++ PCH F QFYVANGE+SC
Sbjct: 343 ARFVIQEFSQLSDVINKIKNNPHDQRIMLAACNPLDFKL-SVSPCHTFTQFYVANGEVSC 402

Query: 69  QMYQRSADMGL--------------------DLAYGDFIHVIGDAHVYRTHVRPLQEQLQ 128
           Q+YQ S +  +                    DL  GDFIHVIG A++ + HV+ +Q+QLQ
Sbjct: 403 QIYQSSTEASIGIPFSIATYSLLTCIIAHVCDLGAGDFIHVIGQAYINKAHVKAIQKQLQ 462

Query: 129 KLPKPFPVLKINPEKKDIDSFVAADFEL 137
             PKPFP+LKINPEKK +D+F A+D EL
Sbjct: 463 ISPKPFPILKINPEKKKMDNFEASDLEL 489

The following BLAST results are available for this feature:

BLAST of Carg26471 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7010921.1	2.3e-83	100.00	Bifunctional dihydrofolate reductase-thymidylate synthase 2, partial [Cucurbita ...	[more]
XP_022944443.1	1.3e-78	88.17	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita moscha...	[more]
XP_022986497.1	5.0e-78	87.57	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita maxima...	[more]
XP_023513067.1	6.5e-78	87.57	bifunctional dihydrofolate reductase-thymidylate synthase-like [Cucurbita pepo s...	[more]
XP_038901596.1	1.8e-75	84.62	bifunctional dihydrofolate reductase-thymidylate synthase 1-like [Benincasa hisp...	[more]

BLAST of Carg26471 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05763	5.9e-74	79.88	Bifunctional dihydrofolate reductase-thymidylate synthase 2 OS=Arabidopsis thali...	[more]
Q05762	6.5e-73	77.51	Bifunctional dihydrofolate reductase-thymidylate synthase 1 OS=Arabidopsis thali...	[more]
P51820	4.0e-70	75.74	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Glycine max OX=3847...	[more]
O81395	8.8e-70	73.81	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Zea mays OX=4577 GN...	[more]
Q2QRX6	1.8e-67	72.62	Putative bifunctional dihydrofolate reductase-thymidylate synthase OS=Oryza sati...	[more]

BLAST of Carg26471 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWP8	6.3e-79	88.17	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita moschata ...	[more]
A0A6J1J7P4	2.4e-78	87.57	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucurbita maxima OX...	[more]
A0A6J1CYC5	8.6e-76	84.02	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Momordica charantia...	[more]
A0A1S3CE37	5.6e-75	84.02	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo OX=365...	[more]
A0A5D3DYK8	5.6e-75	84.02	Bifunctional dihydrofolate reductase-thymidylate synthase OS=Cucumis melo var. m...	[more]

BLAST of Carg26471 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G34570.1	4.2e-75	79.88	thymidylate synthase 2	[more]
AT2G16370.1	4.6e-74	77.51	thymidylate synthase 1	[more]
AT2G21550.1	4.1e-38	53.38	Bifunctional dihydrofolate reductase/thymidylate synthase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000398	Thymidylate synthase	PRINTS	PR00108	THYMDSNTHASE	coord: 66..92 score: 41.77 coord: 22..41 score: 60.96 coord: 48..63 score: 73.54
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	PFAM	PF00303	Thymidylat_synt	coord: 80..151 e-value: 1.6E-20 score: 73.4 coord: 7..82 e-value: 4.3E-29 score: 101.4
IPR023451	Thymidylate synthase/dCMP hydroxymethylase domain	CDD	cd00351	TS_Pyrimidine_HMase	coord: 8..104 e-value: 3.99108E-48 score: 152.815
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 1..80 e-value: 8.0E-37 score: 128.6
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	GENE3D	3.30.572.10	Thymidylate synthase/dCMP hydroxymethylase domain	coord: 81..151 e-value: 3.5E-25 score: 90.7
IPR036926	Thymidylate synthase/dCMP hydroxymethylase superfamily	SUPERFAMILY	55831	Thymidylate synthase/dCMP hydroxymethylase	coord: 3..151
IPR045097	Thymidylate synthase/dCMP hydroxymethylase	PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	coord: 3..81
IPR045097	Thymidylate synthase/dCMP hydroxymethylase	PANTHER	PTHR11548	THYMIDYLATE SYNTHASE 1	coord: 83..151
None	No IPR available	PANTHER	PTHR11548:SF14	BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE	coord: 3..81
None	No IPR available	PANTHER	PTHR11548:SF14	BIFUNCTIONAL DIHYDROFOLATE REDUCTASE-THYMIDYLATE SYNTHASE	coord: 83..151
IPR020940	Thymidylate synthase, active site	PROSITE	PS00091	THYMIDYLATE_SYNTHASE	coord: 33..61

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26471-RA	Carg26471-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006231	dTMP biosynthetic process
biological_process	GO:0032259	methylation
biological_process	GO:0006730	one-carbon metabolic process
biological_process	GO:0046654	tetrahydrofolate biosynthetic process
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004146	dihydrofolate reductase activity
molecular_function	GO:0004799	thymidylate synthase activity
molecular_function	GO:0016741	transferase activity, transferring one-carbon groups