Carg26377 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26377
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	methylesterase 2-like
Location	Carg_Chr11: 8365211 .. 8367181 (+)
RNA-Seq Expression	Carg26377
Synteny	Carg26377

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAAAAGCTTTTGCTATCGTTTTTGGTTCTCATTCTGGTTTTGTTCTCCTCTCCAACGCGGAATTCTGATCAATCTGAATCGACCTATTCGCACTTTGTGCTTGTTCATGGGGCATGTCTTGGCGCTTGGTCATGGTACAAGGTCATTACCCTTTTGCAAACGGCTGGCCACAAAGTGACTGCACTTGACATGGCCGCTGCCGGGATCAACCAGACGCAGCCCGAGTCGTTGGCATCGCTTACTGAGTACTTCGAGCCATTGTTGAGCTTCATGGAAGCGTTGCCTTCAGATGAGCGGATTGTTCTCGTCGGCCACAGCCTTGGCGGGCTCGGCATCTCCATGGCCATGGAGAGTTTCCCTGAGAAAGTGTCTGTTGCCATTTTTGTGACTGCAGCCATGCCTGGTCCCATCGTGGGGTCTTTTACCGTTGAGGAACTGGTTTGAAATCTAACTCAATTTTGTTGGGACAATGAAAGTCCCACGTCGATTAATTTAAGTGATAAAGTTTATTTATTTATTTTTTTTTGTTAGATGAAAATACTTGAAATGCTAAGAGCCTTCTACTCCGAAGAGGATTCCAAAGCTTCTGATTCGAAGCTATTCCTATTCTCCCAAGAAGAATTGAAAACCAAGCTTTTCCCTCTGTCGCCACCTCAGGTTTGTAGTTCTTCCTTTGACTTAATGCTTGAGCTGAGTGAAGAACGGAGGGTTTATAAGTAAGAAATACATGTCGATTGTATGGGCGTTTTGGAAAACTAAAAACAAAGCCATGTGAGCTAATGCTCGAAGTGGACAATATCATATCATTGTGGAGGGTCATGGTTCCTAACATGGTATCAGGGCTATGTCCTTAACCTAGCTATGTCAATAAAATCCTCAAATGTCGAACAAATAAGTTGTGAGTCTCGAAGGTGTAATCAAAAGAGATTCAAGTGTTGAACAAATGGTGTACTTTGTTCGAGAGCTTTAGAGAAAGAGTTGAGCCTCAATTAAGGGGAGACTATTCGAGTACTTCATAGGCCTTAGGGGAGGCTCTATGGTGTACTTTGTTCGAGGAGAGCTGTTCTAGAGCTCCATAGATATCAGGAGAGGCTCTTTGGTGTACTTGTTCAAGGAGAGCTGTTCTAGGGTTCCATAGATCTCAAAAGAGGCTCTATGGTGTACTTCGTTCGAGGGGAGCCGTTCTAGGGCTCCATAGATCTCAGGAGAGGGTCTATGGTGTACTTTGTTCGAGAAGAGCTGTTCTAGGGCTCCATAGATCTCAAGAGAGGTTCTACAGTGTACTTTGTTCAAGGAGAGCTATTCTAGGGCTCCATAGATCTCACAGGGCTCCATAGATCTCACAGAAAGCTCTATGGTGTATTTTGTGCAAGGAAAGGAATTGTTAAGAGATGAGTCCCACCTTGGCTAATTAAGAGGTTGATCATGAGTTTATAAGTAAGGAATACATCTCGTACTTTTTGAAAAAACTAAAAGTAAAGTCATGTGAGCTAACATTCAAATCCGGCAATATCATACCATTATGGAGGGTCGTGGTTCCTAACAAATATCATTTATAACCCGCCAAGTTGAACATTCACACACAAGCTGCGAGACTAAATTGTATAATAAACGTCATGTAAGCTGTAGTATTGACGTGGGTATGTATGTTGGTATATATAGGATGTGGCATTGGCAACAACACTGGAGAGGCCCCAAGCGATGTTCGGCATCTTGGAGTCGATGAAGGAGCTGAGGCTGACAAGAGACAAATACGGGTCGGTGAAAAGGGCTTTCATCATATCAGAAAAGGACGAGATGACATCCAAGATCATGGTGTGGGCAATGCTCACATTCAACAAGCCCGATCGTCAGGCCGAGGTTAAGGGATCAGATCACATGGTCATGGCTTCCAAGCCCCTTGAACTTGCCCAACAACTCACCACCATCGCTCAAGACTTCGCTTCTTCTTCCACATATCATATGTAA

mRNA sequence

ATGCAAAAGCTTTTGCTATCGTTTTTGGTTCTCATTCTGGTTTTGTTCTCCTCTCCAACGCGGAATTCTGATCAATCTGAATCGACCTATTCGCACTTTGTGCTTGTTCATGGGGCATGTCTTGGCGCTTGGTCATGGTACAAGGTCATTACCCTTTTGCAAACGGCTGGCCACAAAGTGACTGCACTTGACATGGCCGCTGCCGGGATCAACCAGACGCAGCCCGAGTCGTTGGCATCGCTTACTGAGTACTTCGAGCCATTGTTGAGCTTCATGGAAGCGTTGCCTTCAGATGAGCGGATTGTTCTCGTCGGCCACAGCCTTGGCGGGCTCGGCATCTCCATGGCCATGGAGAGTTTCCCTGAGAAAGTGTCTGTTGCCATTTTTGTGACTGCAGCCATGCCTGGTCCCATCGTGGGGTCTTTTACCGTTGAGGAACTGATGAAAATACTTGAAATGCTAAGAGCCTTCTACTCCGAAGAGGATTCCAAAGCTTCTGATTCGAAGCTATTCCTATTCTCCCAAGAAGAATTGAAAACCAAGCTTTTCCCTCTGTCGCCACCTCAGGTTTGTAGTTCTTCCTTTGACTTAATGCTTGAGCTGAGTGAAGAACGGAGGGTTTATAAGAGAGCTGTTCTAGGGTTCCATAGATCTCAAAAGAGGCTCTATGGTGTACTTCGTTCGAGGGGAGCCGTTCTAGGGCTCCATAGATCTCAGGAGAGGGTCTATGGTGTACTTTGTTCGAGAAGAGCTGTTCTAGGGCTCCATAGATCTCAAGAGAGGTTCTACAGTGATGTGGCATTGGCAACAACACTGGAGAGGCCCCAAGCGATGTTCGGCATCTTGGAGTCGATGAAGGAGCTGAGGCTGACAAGAGACAAATACGGGTCGGTGAAAAGGGCTTTCATCATATCAGAAAAGGACGAGATGACATCCAAGATCATGGTGTGGGCAATGCTCACATTCAACAAGCCCGATCGTCAGGCCGAGGTTAAGGGATCAGATCACATGGTCATGGCTTCCAAGCCCCTTGAACTTGCCCAACAACTCACCACCATCGCTCAAGACTTCGCTTCTTCTTCCACATATCATATGTAA

Coding sequence (CDS)

ATGCAAAAGCTTTTGCTATCGTTTTTGGTTCTCATTCTGGTTTTGTTCTCCTCTCCAACGCGGAATTCTGATCAATCTGAATCGACCTATTCGCACTTTGTGCTTGTTCATGGGGCATGTCTTGGCGCTTGGTCATGGTACAAGGTCATTACCCTTTTGCAAACGGCTGGCCACAAAGTGACTGCACTTGACATGGCCGCTGCCGGGATCAACCAGACGCAGCCCGAGTCGTTGGCATCGCTTACTGAGTACTTCGAGCCATTGTTGAGCTTCATGGAAGCGTTGCCTTCAGATGAGCGGATTGTTCTCGTCGGCCACAGCCTTGGCGGGCTCGGCATCTCCATGGCCATGGAGAGTTTCCCTGAGAAAGTGTCTGTTGCCATTTTTGTGACTGCAGCCATGCCTGGTCCCATCGTGGGGTCTTTTACCGTTGAGGAACTGATGAAAATACTTGAAATGCTAAGAGCCTTCTACTCCGAAGAGGATTCCAAAGCTTCTGATTCGAAGCTATTCCTATTCTCCCAAGAAGAATTGAAAACCAAGCTTTTCCCTCTGTCGCCACCTCAGGTTTGTAGTTCTTCCTTTGACTTAATGCTTGAGCTGAGTGAAGAACGGAGGGTTTATAAGAGAGCTGTTCTAGGGTTCCATAGATCTCAAAAGAGGCTCTATGGTGTACTTCGTTCGAGGGGAGCCGTTCTAGGGCTCCATAGATCTCAGGAGAGGGTCTATGGTGTACTTTGTTCGAGAAGAGCTGTTCTAGGGCTCCATAGATCTCAAGAGAGGTTCTACAGTGATGTGGCATTGGCAACAACACTGGAGAGGCCCCAAGCGATGTTCGGCATCTTGGAGTCGATGAAGGAGCTGAGGCTGACAAGAGACAAATACGGGTCGGTGAAAAGGGCTTTCATCATATCAGAAAAGGACGAGATGACATCCAAGATCATGGTGTGGGCAATGCTCACATTCAACAAGCCCGATCGTCAGGCCGAGGTTAAGGGATCAGATCACATGGTCATGGCTTCCAAGCCCCTTGAACTTGCCCAACAACTCACCACCATCGCTCAAGACTTCGCTTCTTCTTCCACATATCATATGTAA

Protein sequence

MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSSTYHM

Homology

[Close]

BLAST of Carg26377 vs. NCBI nr
Match: KAG7022435.1 (Methylesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 698.0 bits (1800), Expect = 4.4e-197
Identity = 365/365 (100.00%), Postives = 365/365 (100.00%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF
Sbjct: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT
Sbjct: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE
Sbjct: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
           RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS
Sbjct: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360

Query: 361 STYHM 366
           STYHM
Sbjct: 361 STYHM 365

[Close]

BLAST of Carg26377 vs. NCBI nr
Match: KAG6588637.1 (Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 517.7 bits (1332), Expect = 8.2e-143
Identity = 288/365 (78.90%), Postives = 289/365 (79.18%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLLSFLVLILVLFSSPTR+SDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAME F
Sbjct: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMERF 120

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT
Sbjct: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPPQ                                                   
Sbjct: 181 KLFPLSPPQ--------------------------------------------------- 240

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 241 ------------------------DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 290

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS
Sbjct: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 290

Query: 361 STYHM 366
           STYHM
Sbjct: 361 STYHM 290

[Close]

BLAST of Carg26377 vs. NCBI nr
Match: XP_022931632.1 (methylesterase 2-like [Cucurbita moschata])

HSP 1 Score: 517.3 bits (1331), Expect = 1.1e-142
Identity = 287/365 (78.63%), Postives = 289/365 (79.18%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLLSFLVLILVLFSSPTR+SDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF
Sbjct: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFT+EELMKILEMLRAFYSEEDSKASDS LFLFSQEELKT
Sbjct: 121 PEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAFYSEEDSKASDSNLFLFSQEELKT 180

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPPQ                                                   
Sbjct: 181 KLFPLSPPQ--------------------------------------------------- 240

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 241 ------------------------DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 290

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS
Sbjct: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 290

Query: 361 STYHM 366
           STYHM
Sbjct: 361 STYHM 290

[Close]

BLAST of Carg26377 vs. NCBI nr
Match: XP_022988863.1 (probable esterase PIR7A [Cucurbita maxima])

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-132
Identity = 270/365 (73.97%), Postives = 279/365 (76.44%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLL FLVLILV FSSPT +SDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 10  MQKLLLPFLVLILVSFSSPTWSSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 69

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAME F
Sbjct: 70  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMERF 129

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFT+E+LMKIL+MLRAFYSEEDSKAS+S LFLFSQEEL+ 
Sbjct: 130 PEKVSVAIFVTAAMPGPIVGSFTIEKLMKILQMLRAFYSEEDSKASNSNLFLFSQEELEA 189

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPP+                                                   
Sbjct: 190 KLFPLSPPE--------------------------------------------------- 249

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   D+ LATTL RPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 250 ------------------------DLTLATTLVRPQAMFGILESMKELRLTRDKYGSVKR 299

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTS IMVWAMLTFNKPDRQA VKGSDHMVMASKPL+LAQQLTTIAQDFASS
Sbjct: 310 AFIISEKDEMTSMIMVWAMLTFNKPDRQAVVKGSDHMVMASKPLQLAQQLTTIAQDFASS 299

Query: 361 STYHM 366
           STY M
Sbjct: 370 STYDM 299

[Close]

BLAST of Carg26377 vs. NCBI nr
Match: XP_023530119.1 (methylesterase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 477.2 bits (1227), Expect = 1.2e-130
Identity = 265/365 (72.60%), Postives = 275/365 (75.34%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           M KLLL FLVLI+V FSSPTR+SDQSE TYSHFVLVHGACLGAWSWYKV T LQTAGHKV
Sbjct: 1   MHKLLLPFLVLIVVSFSSPTRSSDQSELTYSHFVLVHGACLGAWSWYKVTTFLQTAGHKV 60

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFME LPSDER+VLVGHSLGGLGISMAME F
Sbjct: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEVLPSDERVVLVGHSLGGLGISMAMERF 120

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFT+E LMKILE LR FYSEEDSKAS+S LFLFSQEEL+T
Sbjct: 121 PEKVSVAIFVTAAMPGPIVGSFTIEHLMKILETLRGFYSEEDSKASNSNLFLFSQEELET 180

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPP+                                                   
Sbjct: 181 KLFPLSPPK--------------------------------------------------- 240

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   DVALATTL RPQAMFGILESMKELRLTR+KYGSVKR
Sbjct: 241 ------------------------DVALATTLVRPQAMFGILESMKELRLTREKYGSVKR 290

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTSKIMVWAMLTFNKPDR+AEVKGSDHMVMASKPL+LAQQLTTIAQDFASS
Sbjct: 301 AFIISEKDEMTSKIMVWAMLTFNKPDREAEVKGSDHMVMASKPLQLAQQLTTIAQDFASS 290

Query: 361 STYHM 366
           S Y M
Sbjct: 361 SKYDM 290

[Close]

BLAST of Carg26377 vs. ExPASy Swiss-Prot
Match: O80475 (Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 1.3e-34
Identity = 107/355 (30.14%), Postives = 167/355 (47.04%), Query Frame = 0


Query: 6   LSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDM 65
           +SF++ +L+     +      E    HFVLVHG+CLGAW WYKV  LL+ +GH+VTALD+
Sbjct: 6   ISFIISLLICGCVKS-----EEMMKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDL 65

Query: 66  AAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVS 125
           AA GI+      +++  +Y EPL+  M +LP+DE++VLVGHS GGL +++AM+ FP+K+S
Sbjct: 66  AACGIDTRSITDISTCEQYSEPLIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKIS 125

Query: 126 VAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLF-LFSQEELKTKLFP 185
           V++FVT+ MP       T      +LE   +  + ED   S+ + + +FS E  K ++  
Sbjct: 126 VSVFVTSFMPD------TKNSPSFVLEKFASTMTPEDWMGSELEPYVVFSAEFTKHRILQ 185

Query: 186 LSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYG 245
           LSP                                                         
Sbjct: 186 LSP--------------------------------------------------------- 245

Query: 246 VLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDK-YGSVKRAFI 305
                               D+ L   L+RP ++F  L  +  ++   +K YGSV RA+I
Sbjct: 246 ------------------IEDLELRLLLKRPGSLF--LNDLSRMKNFSEKGYGSVPRAYI 272

Query: 306 ISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFA 359
           +S+ D   S+     M+    P+   E++G+DH+ +  KP  L+  L  IA  F+
Sbjct: 306 VSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQLLSDHLLAIADKFS 272

[Close]

BLAST of Carg26377 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.7e-34
Identity = 104/324 (32.10%), Postives = 160/324 (49.38%), Query Frame = 0


Query: 32  HFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSF 91
           HFVLVHG CLGAW WYK+  LL++AGHKVTA+D++AAGIN  + + + +  +Y EPL+  
Sbjct: 12  HFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIHTFRDYSEPLMEV 71

Query: 92  MEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKIL 151
           M ++P DE++VL+GHS GG+ + +AME++PEK+SVA+F++A MP P              
Sbjct: 72  MASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDP-------------- 131

Query: 152 EMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRA 211
                         + S  + F +   K           C +  D+ML+           
Sbjct: 132 --------------NHSLTYPFEKYNEK-----------CPA--DMMLD----------- 191

Query: 212 VLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATT 271
                 SQ   YG   + G  + L                 + L   Q     D+ LA  
Sbjct: 192 ------SQFSTYGNPENPGMSMILGPQ-------------FMALKMFQNCSVEDLELAKM 251

Query: 272 LERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDE-MTSKIMVWAMLTFNKPDRQAE 331
           L RP ++F   +  K  + + ++YGSVKRA+I   +D+    +   W + +    D+  E
Sbjct: 252 LTRPGSLF-FQDLAKAKKFSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVG-ADKVKE 262

Query: 332 VKGSDHMVMASKPLELAQQLTTIA 355
           +K +DHM M S+P E+ + L  I+
Sbjct: 312 IKEADHMGMLSQPREVCKCLLDIS 262

[Close]

BLAST of Carg26377 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.2e-34
Identity = 107/335 (31.94%), Postives = 158/335 (47.16%), Query Frame = 0


Query: 27  ESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQP-ESLASLTEYF 86
           E    HFVLVHGAC GAW WYKV  LL+  GH+VTALD+AA+GI+ T+    +++  +Y 
Sbjct: 4   EKRKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYS 63

Query: 87  EPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVG-SFTV 146
           EPL+  M +LP+DE++VLVGHS GGL +++AM+ FP+K+SV++FVTA MP      SF  
Sbjct: 64  EPLMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVE 123

Query: 147 EELMKILEMLRAFYSEEDSKASDSK--LFLFSQEELKTKLFPLSPPQVCSSSFDLMLELS 206
           E+    +       SE ++  SD+      FS + +K +L+ LSP +             
Sbjct: 124 EKFASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVE------------- 183

Query: 207 EERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERF 266
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 267 YSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTF 326
             D+ L   L+RP ++F I E  K    +   YGSV RA+I+ ++D + S+     M+  
Sbjct: 244 --DLELGLLLKRPSSLF-INELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHN 262

Query: 327 NKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDF 358
              +   E++ +DHM M  KP  L+  L  IA +F
Sbjct: 304 YPANLVIEMEETDHMPMFCKPQLLSDHLLAIADNF 262

[Close]

BLAST of Carg26377 vs. ExPASy Swiss-Prot
Match: O80477 (Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.1e-33
Identity = 110/338 (32.54%), Postives = 157/338 (46.45%), Query Frame = 0


Query: 27  ESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQP-ESLASLTEYF 86
           E    H VLVHGAC GAW WYKV   L+ +GH+VTA+D+AA+GI+ T+    +++  +Y 
Sbjct: 4   EERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYS 63

Query: 87  EPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVG-SFTV 146
           EPL+  M +LP DE++VLVGHSLGGL ++MAM+ FP K+SV++FVTA MP      SF  
Sbjct: 64  EPLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVW 123

Query: 147 EELMKIL---EMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLEL 206
           ++L K     E L   ++ E      S+ ++F  E +   L+ LSP Q            
Sbjct: 124 DKLRKETSREEWLDTVFTSE-KPDFPSEFWIFGPEFMAKNLYQLSPVQ------------ 183

Query: 207 SEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQER 266
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 267 FYSDVALATTLERPQAMFGILESMKELR-LTRDKYGSVKRAFIISEKDEMTSKIMVWAML 326
              D+ LA  L R   +  I + M E R  + + YGSV R FI+  KD ++ +    +M+
Sbjct: 244 ---DLELAKMLVRANPL--IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMI 263

Query: 327 TFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFA 359
           +   P    E+K +DHM M SKP +L   L  IA  +A
Sbjct: 304 SNFPPKEVMEIKDADHMPMFSKPQQLCALLLEIANKYA 263

[Close]

BLAST of Carg26377 vs. ExPASy Swiss-Prot
Match: A2WYS8 (Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 2.5e-33
Identity = 109/329 (33.13%), Postives = 151/329 (45.90%), Query Frame = 0


Query: 32  HFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSF 91
           HFV VHG   GAW WY+V+  L+ AGH+ TALDMAAAG +  + + + SL EY  PLL  
Sbjct: 7   HFVFVHGLGHGAWCWYRVVAALRAAGHRATALDMAAAGAHPARADEVGSLEEYSRPLLDA 66

Query: 92  MEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMP--GPIVGSFTVEELMK 151
           + A    ER+VLVGHSLGGL +++AME FP+KV+ A+F+ A MP  G  +G  T+EE M+
Sbjct: 67  VAAAAPGERLVLVGHSLGGLSLALAMERFPDKVAAAVFLAACMPAAGKHMG-ITLEEFMR 126

Query: 152 ILEMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYK 211
            ++                                   P     S  ++L  ++E R   
Sbjct: 127 RIK-----------------------------------PDFFMDSKTIVLNTNQEPRT-- 186

Query: 212 RAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALA 271
                                 +LG     E++Y            +RS      D+ LA
Sbjct: 187 --------------------AVLLGPKLLAEKLY------------NRSPP---EDLTLA 246

Query: 272 TTLERPQAMFGILESMK-ELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQ 331
           T L RP   +     MK E  LT   YGSVKR F+++  D  + + M    +  +     
Sbjct: 247 TMLVRPGTNYIDDPIMKDETLLTEGNYGSVKRVFLVAMDDASSDEEMQRWTIDLSPGVEV 262

Query: 332 AEVKGSDHMVMASKPLELAQQLTTIAQDF 358
            E+ G+DHM M SKP EL   L  IA  +
Sbjct: 307 EELAGADHMAMCSKPRELCDLLLRIAAKY 262

[Close]

BLAST of Carg26377 vs. ExPASy TrEMBL
Match: A0A6J1EU76 (methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1)

HSP 1 Score: 517.3 bits (1331), Expect = 5.2e-143
Identity = 287/365 (78.63%), Postives = 289/365 (79.18%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLLSFLVLILVLFSSPTR+SDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF
Sbjct: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFT+EELMKILEMLRAFYSEEDSKASDS LFLFSQEELKT
Sbjct: 121 PEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAFYSEEDSKASDSNLFLFSQEELKT 180

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPPQ                                                   
Sbjct: 181 KLFPLSPPQ--------------------------------------------------- 240

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 241 ------------------------DVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 290

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS
Sbjct: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 290

Query: 361 STYHM 366
           STYHM
Sbjct: 361 STYHM 290

[Close]

BLAST of Carg26377 vs. ExPASy TrEMBL
Match: A0A6J1JNJ9 (probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.3e-133
Identity = 270/365 (73.97%), Postives = 279/365 (76.44%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 60
           MQKLLL FLVLILV FSSPT +SDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV
Sbjct: 10  MQKLLLPFLVLILVSFSSPTWSSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKV 69

Query: 61  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESF 120
           TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAME F
Sbjct: 70  TALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMERF 129

Query: 121 PEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLFLFSQEELKT 180
           PEKVSVAIFVTAAMPGPIVGSFT+E+LMKIL+MLRAFYSEEDSKAS+S LFLFSQEEL+ 
Sbjct: 130 PEKVSVAIFVTAAMPGPIVGSFTIEKLMKILQMLRAFYSEEDSKASNSNLFLFSQEELEA 189

Query: 181 KLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQE 240
           KLFPLSPP+                                                   
Sbjct: 190 KLFPLSPPE--------------------------------------------------- 249

Query: 241 RVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKR 300
                                   D+ LATTL RPQAMFGILESMKELRLTRDKYGSVKR
Sbjct: 250 ------------------------DLTLATTLVRPQAMFGILESMKELRLTRDKYGSVKR 299

Query: 301 AFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASS 360
           AFIISEKDEMTS IMVWAMLTFNKPDRQA VKGSDHMVMASKPL+LAQQLTTIAQDFASS
Sbjct: 310 AFIISEKDEMTSMIMVWAMLTFNKPDRQAVVKGSDHMVMASKPLQLAQQLTTIAQDFASS 299

Query: 361 STYHM 366
           STY M
Sbjct: 370 STYDM 299

[Close]

BLAST of Carg26377 vs. ExPASy TrEMBL
Match: A0A0A0K4V8 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074970 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 6.8e-95
Identity = 207/378 (54.76%), Postives = 243/378 (64.29%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNS------------DQSESTYSHFVLVHGACLGAWSWYK 60
           MQKLLL F +LI + FS+ T+ S              S+S  SHFVLVHGACLGAWSWY+
Sbjct: 1   MQKLLLPFFLLIFLSFSTQTQTSFLFHHGHHDNDGHDSQSNKSHFVLVHGACLGAWSWYQ 60

Query: 61  VITLLQTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSL 120
           V T LQTAGHKVTA+DMAAAGI+ TQPESL SLT+YF+PLL+F EAL +D++IVLVGHSL
Sbjct: 61  VTTFLQTAGHKVTAVDMAAAGIDPTQPESLTSLTDYFQPLLNFTEALQADDKIVLVGHSL 120

Query: 121 GGLGISMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDS 180
           GGLGISMAME FPEK+SVAIFVTAAMPGPI+G  ++EE    L M +AF+  +D+K+  S
Sbjct: 121 GGLGISMAMERFPEKISVAIFVTAAMPGPIIGFQSIEEQKTTLGMYKAFHKGDDTKSEVS 180

Query: 181 K----LFLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYG 240
           K    LF+FS+EEL+TKLFPLSPPQ                                   
Sbjct: 181 KKPPRLFMFSEEELETKLFPLSPPQ----------------------------------- 240

Query: 241 VLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILES 300
                                                   D+ LA TL RPQAMFG+LES
Sbjct: 241 ----------------------------------------DLTLARTLVRPQAMFGLLES 300

Query: 301 MKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPL 360
           MKELRL+++ YGSVKRAFIIS+ D+MTSK MVWAML  NKPDR  EV GSDHMVM SKPL
Sbjct: 301 MKELRLSKENYGSVKRAFIISQNDKMTSKFMVWAMLLLNKPDRVEEVHGSDHMVMTSKPL 303

Query: 361 ELAQQLTTIAQDFASSST 363
           ELAQ L TIAQD+A+ S+
Sbjct: 361 ELAQLLGTIAQDYAAFSS 303

[Close]

BLAST of Carg26377 vs. ExPASy TrEMBL
Match: A0A1S3C0U9 (methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 4.4e-94
Identity = 206/372 (55.38%), Postives = 246/372 (66.13%), Query Frame = 0


Query: 1   MQKLLLS-FLVLILVLFSSPTRNS------DQSESTYSHFVLVHGACLGAWSWYKVITLL 60
           MQKLLL  F +LI + FS+ T+ S        S+ST SHFVLVHGACLGAWSWYKV T L
Sbjct: 1   MQKLLLPLFFLLIFLSFSTQTQTSFDLLHHHYSQSTKSHFVLVHGACLGAWSWYKVTTFL 60

Query: 61  QTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGI 120
           QTAGH VTALDMAAAGI+ TQP+SL ++T+YF+PLL+FMEAL +DE+IVLVGHSLGGLGI
Sbjct: 61  QTAGHNVTALDMAAAGIDPTQPQSLKTITDYFQPLLNFMEALQADEKIVLVGHSLGGLGI 120

Query: 121 SMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEED--SKASD-SKL 180
           SMAME FPEK+SVAIFVTAAMPGPI   F++EE  K+L M   FY+EED  SK SD S L
Sbjct: 121 SMAMERFPEKISVAIFVTAAMPGPIPWLFSMEEQRKVLGMYGGFYTEEDFKSKGSDNSNL 180

Query: 181 FLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRG 240
           F+FS+EEL+TKLFPLSPP+                                         
Sbjct: 181 FMFSEEELETKLFPLSPPE----------------------------------------- 240

Query: 241 AVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRL 300
                                             D+ LA TL RPQAMFG+ ES+KEL+L
Sbjct: 241 ----------------------------------DLTLAKTLVRPQAMFGLFESVKELKL 297

Query: 301 TRDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQL 360
           +++ YGSVKRAFIISEKD+M +KIMVWAM+  N+P    EV GSDHMVM SKPLELA+QL
Sbjct: 301 SKENYGSVKRAFIISEKDKMATKIMVWAMIISNRPHHVGEVHGSDHMVMTSKPLELARQL 297

Query: 361 TTIAQDFASSST 363
           +TIAQDFA++++
Sbjct: 361 STIAQDFAAAAS 297

[Close]

BLAST of Carg26377 vs. ExPASy TrEMBL
Match: A0A6J1C2S8 (methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 2.9e-93
Identity = 207/371 (55.80%), Postives = 239/371 (64.42%), Query Frame = 0


Query: 1   MQKLLLSFLVLILVLFSSPTRNSD-------QSESTYSHFVLVHGACLGAWSWYKVITLL 60
           +  LL   L+LIL+  SSPT++S        QS S   HFVLVHGACLGAWSWYKV T L
Sbjct: 15  LHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYL 74

Query: 61  QTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGI 120
           Q AGH VTALDMA AGIN TQP+SL+SLT+Y+EPLL FM+ALPSDE++VLVGHSLGGLGI
Sbjct: 75  QLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGI 134

Query: 121 SMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAF--YSEEDSKASDSKLF 180
           SMAME FPEK+SVAIFVTAAMPGPI     +EE  KILEM   F    EE SK SD+KLF
Sbjct: 135 SMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLF 194

Query: 181 LFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGA 240
           +F++E+L TKLFPLSPP+                                          
Sbjct: 195 IFTEEQLATKLFPLSPPE------------------------------------------ 254

Query: 241 VLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLT 300
                                            D+ LATTL RPQ MF +++SM+EL LT
Sbjct: 255 ---------------------------------DLRLATTLVRPQPMFDLIKSMEELDLT 307

Query: 301 RDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLT 360
           RD YGSVKRAFIISE DEM  +IMVWAML  NKPDR AE+KGSDHMVMASKPL+LAQQL+
Sbjct: 315 RDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLS 307

Query: 361 TIAQDFASSST 363
           TIA +FASS++
Sbjct: 375 TIALEFASSTS 307

[Close]

BLAST of Carg26377 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 148.7 bits (374), Expect = 9.3e-36
Identity = 107/355 (30.14%), Postives = 167/355 (47.04%), Query Frame = 0


Query: 6   LSFLVLILVLFSSPTRNSDQSESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDM 65
           +SF++ +L+     +      E    HFVLVHG+CLGAW WYKV  LL+ +GH+VTALD+
Sbjct: 6   ISFIISLLICGCVKS-----EEMMKQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDL 65

Query: 66  AAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVS 125
           AA GI+      +++  +Y EPL+  M +LP+DE++VLVGHS GGL +++AM+ FP+K+S
Sbjct: 66  AACGIDTRSITDISTCEQYSEPLIQLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKIS 125

Query: 126 VAIFVTAAMPGPIVGSFTVEELMKILEMLRAFYSEEDSKASDSKLF-LFSQEELKTKLFP 185
           V++FVT+ MP       T      +LE   +  + ED   S+ + + +FS E  K ++  
Sbjct: 126 VSVFVTSFMPD------TKNSPSFVLEKFASTMTPEDWMGSELEPYVVFSAEFTKHRILQ 185

Query: 186 LSPPQVCSSSFDLMLELSEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYG 245
           LSP                                                         
Sbjct: 186 LSP--------------------------------------------------------- 245

Query: 246 VLCSRRAVLGLHRSQERFYSDVALATTLERPQAMFGILESMKELRLTRDK-YGSVKRAFI 305
                               D+ L   L+RP ++F  L  +  ++   +K YGSV RA+I
Sbjct: 246 ------------------IEDLELRLLLKRPGSLF--LNDLSRMKNFSEKGYGSVPRAYI 272

Query: 306 ISEKDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFA 359
           +S+ D   S+     M+    P+   E++G+DH+ +  KP  L+  L  IA  F+
Sbjct: 306 VSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQLLSDHLLAIADKFS 272

[Close]

BLAST of Carg26377 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 147.9 bits (372), Expect = 1.6e-35
Identity = 107/335 (31.94%), Postives = 158/335 (47.16%), Query Frame = 0


Query: 27  ESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQP-ESLASLTEYF 86
           E    HFVLVHGAC GAW WYKV  LL+  GH+VTALD+AA+GI+ T+    +++  +Y 
Sbjct: 4   EKRKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYS 63

Query: 87  EPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVG-SFTV 146
           EPL+  M +LP+DE++VLVGHS GGL +++AM+ FP+K+SV++FVTA MP      SF  
Sbjct: 64  EPLMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVE 123

Query: 147 EELMKILEMLRAFYSEEDSKASDSK--LFLFSQEELKTKLFPLSPPQVCSSSFDLMLELS 206
           E+    +       SE ++  SD+      FS + +K +L+ LSP +             
Sbjct: 124 EKFASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVE------------- 183

Query: 207 EERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERF 266
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 267 YSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTF 326
             D+ L   L+RP ++F I E  K    +   YGSV RA+I+ ++D + S+     M+  
Sbjct: 244 --DLELGLLLKRPSSLF-INELSKMENFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHN 262

Query: 327 NKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDF 358
              +   E++ +DHM M  KP  L+  L  IA +F
Sbjct: 304 YPANLVIEMEETDHMPMFCKPQLLSDHLLAIADNF 262

[Close]

BLAST of Carg26377 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 145.6 bits (366), Expect = 7.9e-35
Identity = 110/338 (32.54%), Postives = 157/338 (46.45%), Query Frame = 0


Query: 27  ESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQP-ESLASLTEYF 86
           E    H VLVHGAC GAW WYKV   L+ +GH+VTA+D+AA+GI+ T+    +++  +Y 
Sbjct: 4   EERKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYS 63

Query: 87  EPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVG-SFTV 146
           EPL+  M +LP DE++VLVGHSLGGL ++MAM+ FP K+SV++FVTA MP      SF  
Sbjct: 64  EPLMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVW 123

Query: 147 EELMKIL---EMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLEL 206
           ++L K     E L   ++ E      S+ ++F  E +   L+ LSP Q            
Sbjct: 124 DKLRKETSREEWLDTVFTSE-KPDFPSEFWIFGPEFMAKNLYQLSPVQ------------ 183

Query: 207 SEERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQER 266
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 267 FYSDVALATTLERPQAMFGILESMKELR-LTRDKYGSVKRAFIISEKDEMTSKIMVWAML 326
              D+ LA  L R   +  I + M E R  + + YGSV R FI+  KD ++ +    +M+
Sbjct: 244 ---DLELAKMLVRANPL--IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMI 263

Query: 327 TFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFA 359
           +   P    E+K +DHM M SKP +L   L  IA  +A
Sbjct: 304 SNFPPKEVMEIKDADHMPMFSKPQQLCALLLEIANKYA 263

[Close]

BLAST of Carg26377 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 136.7 bits (343), Expect = 3.7e-32
Identity = 103/326 (31.60%), Postives = 153/326 (46.93%), Query Frame = 0


Query: 32  HFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSF 91
           HFV VHG+C GAW W+K+   L+  GH+VTA+D+  +G++  Q   +  ++ Y EPL+SF
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 92  MEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVGSFTVEELMKIL 151
           ME+LP +E++VLVGHS GG+G S+AME FP KVSV IF++A MP                
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMP---------------- 153

Query: 152 EMLRAFYSEEDSKASDSKLFLFSQEELKTKLFPLSPPQVCSSSFDLMLELSEERRVYKRA 211
                          DS   +  QE   T+L P      C  +F+  LE           
Sbjct: 154 -------------HHDSPPAVLIQEYF-TRL-PEGFAMDCEFTFEEGLE----------- 213

Query: 212 VLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERFYSDVALATT 271
               H     L+G              +E+ Y   C                 D+ LA  
Sbjct: 214 ----HPPSSVLFGT----------SFLKEKAYS-NCQ--------------LEDLELAMA 273

Query: 272 LERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTFNKPDRQAEV 331
           L +P  ++   E   E  +T+++YGS KR FI+ E D +  + +   M++  +P     +
Sbjct: 274 LMKPSWLY-TKEMGGEDLITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRI 287

Query: 332 KGSDHMVMASKPLELAQQLTTIAQDF 358
           + + HM M +KP EL+Q L  IA  +
Sbjct: 334 EEAGHMAMLTKPHELSQLLQEIAAKY 287

[Close]

BLAST of Carg26377 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 136.0 bits (341), Expect = 6.2e-32
Identity = 101/335 (30.15%), Postives = 152/335 (45.37%), Query Frame = 0


Query: 27  ESTYSHFVLVHGACLGAWSWYKVITLLQTAGHKVTALDMAAAGINQTQP-ESLASLTEYF 86
           E    HFVLVHG+C GAW WYKV  LL+  GH+VTA+D+AA+GI+ T+    + +  +Y 
Sbjct: 4   EKRKQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYS 63

Query: 87  EPLLSFMEALPSDERIVLVGHSLGGLGISMAMESFPEKVSVAIFVTAAMPGPIVG-SFTV 146
           EPL   + +LP+DE++VLVGHS GGL +++AME FPEK+SVA+F+TA MP      SF +
Sbjct: 64  EPLTKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVL 123

Query: 147 EELMKILEMLRAFYSEEDSKASDSK--LFLFSQEELKTKLFPLSPPQVCSSSFDLMLELS 206
           ++    +       +E +   SD+      FS + +K  L+ LSP +             
Sbjct: 124 DKFGSNMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVE------------- 183

Query: 207 EERRVYKRAVLGFHRSQKRLYGVLRSRGAVLGLHRSQERVYGVLCSRRAVLGLHRSQERF 266
                                                                       
Sbjct: 184 ------------------------------------------------------------ 243

Query: 267 YSDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDEMTSKIMVWAMLTF 326
             D+ L   L RP ++F I +  K    + + YGSV R FI+ ++D+   +     M+  
Sbjct: 244 --DLELGLLLMRPGSLF-INDLSKMKNFSDEGYGSVPRVFIVCKEDKAIPEERQRWMIDN 262

Query: 327 NKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDF 358
              +   E++ +DHM M  KP +L+     IA  F
Sbjct: 304 FPVNLVMEMEETDHMPMFCKPQQLSDYFLKIADKF 262

The following BLAST results are available for this feature:

BLAST of Carg26377 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022435.1	4.4e-197	100.00	Methylesterase 8, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6588637.1	8.2e-143	78.90	Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022931632.1	1.1e-142	78.63	methylesterase 2-like [Cucurbita moschata]	[more]
XP_022988863.1	1.3e-132	73.97	probable esterase PIR7A [Cucurbita maxima]	[more]
XP_023530119.1	1.2e-130	72.60	methylesterase 3-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg26377 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80475	1.3e-34	30.14	Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1	[more]
Q9SE93	1.7e-34	32.10	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]
O80476	2.2e-34	31.94	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]
O80477	1.1e-33	32.54	Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1	[more]
A2WYS8	2.5e-33	33.13	Probable esterase PIR7A OS=Oryza sativa subsp. indica OX=39946 GN=PIR7A PE=3 SV=...	[more]

BLAST of Carg26377 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EU76	5.2e-143	78.63	methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1	[more]
A0A6J1JNJ9	6.3e-133	73.97	probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1	[more]
A0A0A0K4V8	6.8e-95	54.76	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074...	[more]
A0A1S3C0U9	4.4e-94	55.38	methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1	[more]
A0A6J1C2S8	2.9e-93	55.80	methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1	[more]

BLAST of Carg26377 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23590.1	9.3e-36	30.14	methyl esterase 8	[more]
AT2G23600.1	1.6e-35	31.94	acetone-cyanohydrin lyase	[more]
AT2G23610.1	7.9e-35	32.54	methyl esterase 3	[more]
AT3G50440.1	3.7e-32	31.60	methyl esterase 10	[more]
AT2G23620.1	6.2e-32	30.15	methyl esterase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 33..348 e-value: 1.2E-12 score: 48.9
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 29..358 e-value: 7.6E-50 score: 172.1
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 30..352
None	No IPR available	PANTHER	PTHR10992:SF1002	ESTERASE PIR7A-RELATED	coord: 19..194 coord: 264..356
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 19..194 coord: 264..356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26377-RA	Carg26377-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032787	monocarboxylic acid metabolic process
molecular_function	GO:0052689	carboxylic ester hydrolase activity