Carg26376 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26376
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DNL-type domain-containing protein
Location	Carg_Chr11: 11331783 .. 11332968 (-)
RNA-Seq Expression	Carg26376
Synteny	Carg26376

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGTGGCTTTTACTTGTAACATTTGTGGGCAACGTACAACACGGGCTATTAACCCCCATGCTTATACCGATGGCACTGTGTTTGTGCAGGTAAGATTTCACAACACTTATGGAATATTCGATGTAAGCGCATATATTGAGTTGGCAGAGTAGAAGAATACAGTTACCATTTTTTTGTTCGGTTTTCTATTGAAATGGTTTCAGAAATATTGGGGGAAGAATTGATGTCAAAAGAAATGGCCTAAGCCCACCGCTAACAAGATATTGTCTTATTTGGATTTTCCCTTCTAGATTTTTAGAACGCGTCTGCTAGGGAGAGGTTTCCACACTCATATAAAAAATGCTTTGTTCCCCTCTTCAACCAATGTGGGATTCCGCAATCCATCTCCCTTCTGGGCCCAACGTTCTCGTTAGCACTCGTTCCCTTCTCCAACCGATAGATTGGGACTCCCCAATCCATCCTTTTTGAGGCCCAGTGTCCTTGTTGGCACACGGCCTCGTGTCCACCCCCTTATATCATCTGTAATGGCTTAAGTCCATTGCTAAAACACGTTTTTCATGGAGAGGTTTGCACACCCTTATAAAGAATGCTTCATTCCCCTCCCCGACTAATGTGAGATCTCACACATCATCTTTGAATTCAGCTACCAACCCAGTGAGACTTCTGCATGTAATTGGCTGATATTCTTGCAGCTATTCTATCTTAGTTGTTAGGTCCAATAAAGTGTGTTGCCTAAAGCAAGCTTGAGTAGACAAATCTGATTTAAATTTGCGGGTAAACAATTTTATTACAAACTGACACAAAACTGATCATCTTAATCTTTTGTTTTGTTGTTGCAGTGTTGTGGATGCAGTGCATACCACAAGCTTGTGGACAACTTAAACCTGTTTCATGAGATGAAATGCTACGTCAACCCGAGTTTTAACTATGGGAGTAATGGGTGGGGCGATTTCAACTTCAAATATCTGGACATGGAAGAAGATGGAAGCAACGACGATATATTCCCAACACAATGAGGTAAATGCCCTGTGCTCATGGAGTATGTACATTCTGGACTCATCTGTACAAGAACCATAGAACCATTATTTGTTAGGCTAAATTATAAAAAATACTCCTTTGTGTGTTTCAAAAGTATCTTCTTTCAAAAATTGCAATTTTACTCTTTGATAAATCGTAACCATT

mRNA sequence

ATGCTTGTGGCTTTTACTTGTAACATTTGTGGGCAACGTACAACACGGGCTATTAACCCCCATGCTTATACCGATGGCACTGTGTTTGTGCAGTGTTGTGGATGCAGTGCATACCACAAGCTTGTGGACAACTTAAACCTGTTTCATGAGATGAAATGCTACGTCAACCCGAGTTTTAACTATGGGAGTAATGGGTGGGGCGATTTCAACTTCAAATATCTGGACATGGAAGAAGATGGAAGCAACGACGATATATTCCCAACACAATGAGGTAAATGCCCTGTGCTCATGGAGTATGTACATTCTGGACTCATCTGTACAAGAACCATAGAACCATTATTTGTTAGGCTAAATTATAAAAAATACTCCTTTGTGTGTTTCAAAAGTATCTTCTTTCAAAAATTGCAATTTTACTCTTTGATAAATCGTAACCATT

Coding sequence (CDS)

ATGCTTGTGGCTTTTACTTGTAACATTTGTGGGCAACGTACAACACGGGCTATTAACCCCCATGCTTATACCGATGGCACTGTGTTTGTGCAGTGTTGTGGATGCAGTGCATACCACAAGCTTGTGGACAACTTAAACCTGTTTCATGAGATGAAATGCTACGTCAACCCGAGTTTTAACTATGGGAGTAATGGGTGGGGCGATTTCAACTTCAAATATCTGGACATGGAAGAAGATGGAAGCAACGACGATATATTCCCAACACAATGA

Protein sequence

MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFNYGSNGWGDFNFKYLDMEEDGSNDDIFPTQ

Homology

[Close]

BLAST of Carg26376 vs. NCBI nr
Match: KAG7022738.1 (ZIM17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 200.3 bits (508), Expect = 7.0e-48
Identity = 89/89 (100.00%), Postives = 89/89 (100.00%), Query Frame = 0


Query: 1  MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
          MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 1  MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60

Query: 61 YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
          YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ
Sbjct: 61 YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 89

[Close]

BLAST of Carg26376 vs. NCBI nr
Match: XP_022928140.1 (uncharacterized protein LOC111435053 [Cucurbita moschata] >XP_022928141.1 uncharacterized protein LOC111435053 [Cucurbita moschata])

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 87/89 (97.75%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHE+KCYVNPSFN
Sbjct: 129 MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHELKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSNDDIFPTQ
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSNDDIFPTQ 217

[Close]

BLAST of Carg26376 vs. NCBI nr
Match: XP_023529786.1 (uncharacterized protein LOC111792506 [Cucurbita pepo subsp. pepo] >XP_023529787.1 uncharacterized protein LOC111792506 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 189.1 bits (479), Expect = 1.6e-44
Identity = 86/89 (96.63%), Postives = 86/89 (96.63%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 129 MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YG NGWGD NFKYLDMEEDGSN DIFPTQ
Sbjct: 189 YGRNGWGDVNFKYLDMEEDGSN-DIFPTQ 216

[Close]

BLAST of Carg26376 vs. NCBI nr
Match: XP_022989270.1 (uncharacterized protein LOC111486391 isoform X1 [Cucurbita maxima])

HSP 1 Score: 186.8 bits (473), Expect = 8.1e-44
Identity = 85/89 (95.51%), Postives = 85/89 (95.51%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTC ICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 129 MLVAFTCKICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSN DIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSN-DIFPNQ 216

[Close]

BLAST of Carg26376 vs. NCBI nr
Match: XP_022989271.1 (uncharacterized protein LOC111486391 isoform X2 [Cucurbita maxima])

HSP 1 Score: 186.8 bits (473), Expect = 8.1e-44
Identity = 85/89 (95.51%), Postives = 85/89 (95.51%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTC ICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 129 MLVAFTCKICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSN DIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSN-DIFPNQ 216

[Close]

BLAST of Carg26376 vs. ExPASy Swiss-Prot
Match: Q9D113 (DNL-type zinc finger protein OS=Mus musculus OX=10090 GN=Dnlz PE=1 SV=1)

HSP 1 Score: 53.1 bits (126), Expect = 1.8e-06
Identity = 20/50 (40.00%), Postives = 32/50 (64.00%), Query Frame = 0


Query: 3   VAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMK 53
           + +TC +CG R+++ I+  AY  G V V C GC  +H + DNL+ F ++K
Sbjct: 75  LVYTCKVCGTRSSKRISKLAYHQGVVIVTCPGCQNHHIIADNLSWFSDLK 124

[Close]

BLAST of Carg26376 vs. ExPASy Swiss-Prot
Match: Q5SXM8 (DNL-type zinc finger protein OS=Homo sapiens OX=9606 GN=DNLZ PE=1 SV=1)

HSP 1 Score: 50.8 bits (120), Expect = 9.1e-06
Identity = 19/49 (38.78%), Postives = 30/49 (61.22%), Query Frame = 0


Query: 3   VAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEM 52
           + +TC +CG R+++ I+  AY  G V V C GC  +H + DNL  F ++
Sbjct: 71  LVYTCKVCGTRSSKRISKLAYHQGVVIVTCPGCQNHHIIADNLGWFSDL 119

[Close]

BLAST of Carg26376 vs. ExPASy Swiss-Prot
Match: Q09759 (Uncharacterized protein C24H6.02c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC24H6.02c PE=4 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 2.6e-05
Identity = 20/48 (41.67%), Postives = 28/48 (58.33%), Query Frame = 0


Query: 3   VAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHE 51
           V+FTC +C  R+    +  AY +GTV VQC  C   H + D+L +F E
Sbjct: 78  VSFTCTVCNTRSNHNFSKQAYHNGTVLVQCPKCKNRHLMADHLKIFSE 125

[Close]

BLAST of Carg26376 vs. ExPASy TrEMBL
Match: A0A6J1EQU5 (uncharacterized protein LOC111435053 OS=Cucurbita moschata OX=3662 GN=LOC111435053 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 4.9e-47
Identity = 87/89 (97.75%), Postives = 88/89 (98.88%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHE+KCYVNPSFN
Sbjct: 129 MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHELKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSNDDIFPTQ
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSNDDIFPTQ 217

[Close]

BLAST of Carg26376 vs. ExPASy TrEMBL
Match: A0A6J1JJL2 (uncharacterized protein LOC111486391 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111486391 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-44
Identity = 85/89 (95.51%), Postives = 85/89 (95.51%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTC ICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 129 MLVAFTCKICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSN DIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSN-DIFPNQ 216

[Close]

BLAST of Carg26376 vs. ExPASy TrEMBL
Match: A0A6J1JLW6 (uncharacterized protein LOC111486391 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111486391 PE=4 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.9e-44
Identity = 85/89 (95.51%), Postives = 85/89 (95.51%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTC ICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN
Sbjct: 129 MLVAFTCKICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLDMEEDGSN DIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDMEEDGSN-DIFPNQ 216

[Close]

BLAST of Carg26376 vs. ExPASy TrEMBL
Match: A0A5D3D712 (Mitochondrial protein import protein ZIM17 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold3037G00120 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.7e-44
Identity = 83/89 (93.26%), Postives = 86/89 (96.63%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGC+AYHKLVDNLNLFHEMKCY+NPSFN
Sbjct: 129 MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCNAYHKLVDNLNLFHEMKCYINPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLD+EEDG NDDIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDVEEDG-NDDIFPIQ 216

[Close]

BLAST of Carg26376 vs. ExPASy TrEMBL
Match: A0A1S3BII6 (uncharacterized protein LOC103490262 OS=Cucumis melo OX=3656 GN=LOC103490262 PE=4 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 6.7e-44
Identity = 83/89 (93.26%), Postives = 86/89 (96.63%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYVNPSFN 60
           MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGC+AYHKLVDNLNLFHEMKCY+NPSFN
Sbjct: 129 MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCNAYHKLVDNLNLFHEMKCYINPSFN 188

Query: 61  YGSNGWGDFNFKYLDMEEDGSNDDIFPTQ 90
           YGSNGWGD NFKYLD+EEDG NDDIFP Q
Sbjct: 189 YGSNGWGDVNFKYLDVEEDG-NDDIFPIQ 216

[Close]

BLAST of Carg26376 vs. TAIR 10
Match: AT5G27280.1 (Zim17-type zinc finger protein )

HSP 1 Score: 125.2 bits (313), Expect = 2.7e-29
Identity = 59/91 (64.84%), Postives = 69/91 (75.82%), Query Frame = 0


Query: 1   MLVAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHEMKCYV-NPSF 60
           M VAFTCN+CGQRTTRAINPHAYTDGTVFVQCCGC+ +HKLVDNLNLFHE+K YV + SF
Sbjct: 121 MRVAFTCNVCGQRTTRAINPHAYTDGTVFVQCCGCNVFHKLVDNLNLFHEVKYYVSSSSF 180

Query: 61  NYGSNGWGDFNFKYLDMEED---GSNDDIFP 88
           +Y    W        D E+D   G ++D+FP
Sbjct: 181 DYTDAKWDVSGLNLFDDEDDDNAGDSNDVFP 211

[Close]

BLAST of Carg26376 vs. TAIR 10
Match: AT1G68730.1 (Zim17-type zinc finger protein )

HSP 1 Score: 71.6 bits (174), Expect = 3.5e-13
Identity = 32/48 (66.67%), Postives = 34/48 (70.83%), Query Frame = 0


Query: 3   VAFTCNICGQRTTRAINPHAYTDGTVFVQCCGCSAYHKLVDNLNLFHE 51
           V FTCN CG+RT R IN HAY  G VFVQC GC  +HKLVDNL L  E
Sbjct: 108 VEFTCNSCGERTKRLINRHAYEKGLVFVQCAGCLKHHKLVDNLGLIVE 155

The following BLAST results are available for this feature:

BLAST of Carg26376 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022738.1	7.0e-48	100.00	ZIM17, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022928140.1	1.0e-46	97.75	uncharacterized protein LOC111435053 [Cucurbita moschata] >XP_022928141.1 unchar...	[more]
XP_023529786.1	1.6e-44	96.63	uncharacterized protein LOC111792506 [Cucurbita pepo subsp. pepo] >XP_023529787....	[more]
XP_022989270.1	8.1e-44	95.51	uncharacterized protein LOC111486391 isoform X1 [Cucurbita maxima]	[more]
XP_022989271.1	8.1e-44	95.51	uncharacterized protein LOC111486391 isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg26376 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9D113	1.8e-06	40.00	DNL-type zinc finger protein OS=Mus musculus OX=10090 GN=Dnlz PE=1 SV=1	[more]
Q5SXM8	9.1e-06	38.78	DNL-type zinc finger protein OS=Homo sapiens OX=9606 GN=DNLZ PE=1 SV=1	[more]
Q09759	2.6e-05	41.67	Uncharacterized protein C24H6.02c OS=Schizosaccharomyces pombe (strain 972 / ATC...	[more]

BLAST of Carg26376 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EQU5	4.9e-47	97.75	uncharacterized protein LOC111435053 OS=Cucurbita moschata OX=3662 GN=LOC1114350...	[more]
A0A6J1JJL2	3.9e-44	95.51	uncharacterized protein LOC111486391 isoform X2 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1JLW6	3.9e-44	95.51	uncharacterized protein LOC111486391 isoform X1 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A5D3D712	6.7e-44	93.26	Mitochondrial protein import protein ZIM17 OS=Cucumis melo var. makuwa OX=119469...	[more]
A0A1S3BII6	6.7e-44	93.26	uncharacterized protein LOC103490262 OS=Cucumis melo OX=3656 GN=LOC103490262 PE=...	[more]

BLAST of Carg26376 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27280.1	2.7e-29	64.84	Zim17-type zinc finger protein	[more]
AT1G68730.1	3.5e-13	66.67	Zim17-type zinc finger protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR007853	Zinc finger, DNL-type	PFAM	PF05180	zf-DNL	coord: 3..52 e-value: 5.0E-17 score: 61.6
IPR007853	Zinc finger, DNL-type	PROSITE	PS51501	ZF_DNL	coord: 1..89 score: 13.936144
IPR024158	Mitochondrial import protein TIM15	PANTHER	PTHR20922	UNCHARACTERIZED	coord: 1..88
None	No IPR available	PANTHER	PTHR20922:SF15	A_TM021B04.14 PROTEIN	coord: 1..88

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26376-RA	Carg26376-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006457	protein folding
biological_process	GO:0030150	protein import into mitochondrial matrix
biological_process	GO:0050821	protein stabilization
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051087	chaperone binding
molecular_function	GO:0008270	zinc ion binding