Carg26033 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg26033
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionN-acetyltransferase domain-containing protein
LocationCarg_Chr13: 238075 .. 239473 (-)
RNA-Seq ExpressionCarg26033
SyntenyCarg26033
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTTTGTGTCAACGAAGAAGTGGGGAGAGAAAAATGGGATGCGAAGAAGAGATTTTGATAATAAGAAGCTATGATGGACAATATGCAGATAGAGTTAGAGTGGAAGATCTAGAGAGAAGATGTGAGGTAGGCCCATCTGAAAGAGTTTTTCTCTTCACAGACACTATGGGAGACCCCATTTGTAGGATCAGAAACAGTCCCTTGTACAAGATGCTGGTATTTCCTCTCACCACTTCCCTTCCTTCTTCTTTATATATACACATCCTAGTTTACAACTTAGCTTTTCGTTACTTTAGGTGGCGGAAGTTGACAACCAGTTGGTCGGTGTGATTCAAGGCTCGATTAAGGTAGTCACGGTTCATCAAGCACAGAAAGACCGGGCCAAGGTTGGCTATGTTTTAGGTCTTCGAGTTGCGCCGTCGTTTCGCCGTAGAGGGATTGGTTGCAACCTTGTCCTGCGTCTCGAGGAATGGTTCGTGGCCAATGATGTAGAGTATACTTATATGGCAACGGAGAAAGACAATGAAGCCTCTGTGAAACTATTCATCTACAAGCTTGGATACACCAACTTTAGAGTTCCGGCGATTTTGGTGAACCCAGTGAAACATTACCGATCATATCACCTCCCTTCCAATATCCAAATCTCTCGTCTTAAAGTCGGGGTTGCCGAGTATCTCTACCGAAAATTCATGGCCTCTACCGAGTTTTTCCCCCACGACATTGATCAAGTTCTCAGACACAAGCTAAGCCTCGGTACATGGGTTGCTTACTATAGAGACGACACCGACGATGCCGAGGACGCCAATTTTGAAACAAGTGGCAGCAAATCAGAAATGACAATACCAAAGAGCTGGGCAATGCTGAGCGTATGGAATAGTGGAGAGGTGAGTGTTAAACTACAACTTAACCAAAGAAGTTTGCTTGCTAAGCCAATAAGTTATTTACTCATTTCATTAATTTTCTCGCCAAGGTATTCAAGTTGCGGCTTGGGAAGGCACCATTGTCATGCTTGATATACACAGAGAGCTCAAAGGTGATAGACAAGATCTTCCCATGCCTAAAGTTACCATCAATACCAAATTTCTACGAACCATTCGGATTCTACTTTATGTACGGGGTTCACCGAGAGGGGGCAAGGACAGGGAAGCTGGTGAGGGCATTGTGCCAGTACGTGCACAACGTGGCGGCCACAACAAGGGACTGTAAAGCAATAGTAACAGAGATTGGAGGAGAAGATTCACTAAGAGAGGAGATTCCACATTGGAAATTGCTATCATGCCCTGAAGATTTATGGTGCATAAAGGGATTGAAGAAAGAAGCAAGAAATAGCCTTCACGAGTTGACAAAAACCCCATCAACTACAAGACCAGCCCTTTTTGTAGACCCAAGAGAAGTATGA

mRNA sequence

TTTTTGTGTCAACGAAGAAGTGGGGAGAGAAAAATGGGATGCGAAGAAGAGATTTTGATAATAAGAAGCTATGATGGACAATATGCAGATAGAGTTAGAGTGGAAGATCTAGAGAGAAGATGTGAGGTAGGCCCATCTGAAAGAGTTTTTCTCTTCACAGACACTATGGGAGACCCCATTTGTAGGATCAGAAACAGTCCCTTGTACAAGATGCTGGTGGCGGAAGTTGACAACCAGTTGGTCGGTGTGATTCAAGGCTCGATTAAGGTAGTCACGGTTCATCAAGCACAGAAAGACCGGGCCAAGGTTGGCTATGTTTTAGGTCTTCGAGTTGCGCCGTCGTTTCGCCGTAGAGGGATTGGTTGCAACCTTGTCCTGCGTCTCGAGGAATGGTTCGTGGCCAATGATGTAGAGTATACTTATATGGCAACGGAGAAAGACAATGAAGCCTCTGTGAAACTATTCATCTACAAGCTTGGATACACCAACTTTAGAGTTCCGGCGATTTTGGTGAACCCAGTGAAACATTACCGATCATATCACCTCCCTTCCAATATCCAAATCTCTCGTCTTAAAGTCGGGGTTGCCGAGTATCTCTACCGAAAATTCATGGCCTCTACCGAGTTTTTCCCCCACGACATTGATCAAGTTCTCAGACACAAGCTAAGCCTCGGTACATGGGTTGCTTACTATAGAGACGACACCGACGATGCCGAGGACGCCAATTTTGAAACAAGTGGCAGCAAATCAGAAATGACAATACCAAAGAGCTGGGCAATGCTGAGCGTATGGAATAGTGGAGAGGTATTCAAGTTGCGGCTTGGGAAGGCACCATTGTCATGCTTGATATACACAGAGAGCTCAAAGGTGATAGACAAGATCTTCCCATGCCTAAAGTTACCATCAATACCAAATTTCTACGAACCATTCGGATTCTACTTTATGTACGGGGTTCACCGAGAGGGGGCAAGGACAGGGAAGCTGGTGAGGGCATTGTGCCAGTACGTGCACAACGTGGCGGCCACAACAAGGGACTGTAAAGCAATAGTAACAGAGATTGGAGGAGAAGATTCACTAAGAGAGGAGATTCCACATTGGAAATTGCTATCATGCCCTGAAGATTTATGGTGCATAAAGGGATTGAAGAAAGAAGCAAGAAATAGCCTTCACGAGTTGACAAAAACCCCATCAACTACAAGACCAGCCCTTTTTGTAGACCCAAGAGAAGTATGA

Coding sequence (CDS)

TTTTTGTGTCAACGAAGAAGTGGGGAGAGAAAAATGGGATGCGAAGAAGAGATTTTGATAATAAGAAGCTATGATGGACAATATGCAGATAGAGTTAGAGTGGAAGATCTAGAGAGAAGATGTGAGGTAGGCCCATCTGAAAGAGTTTTTCTCTTCACAGACACTATGGGAGACCCCATTTGTAGGATCAGAAACAGTCCCTTGTACAAGATGCTGGTGGCGGAAGTTGACAACCAGTTGGTCGGTGTGATTCAAGGCTCGATTAAGGTAGTCACGGTTCATCAAGCACAGAAAGACCGGGCCAAGGTTGGCTATGTTTTAGGTCTTCGAGTTGCGCCGTCGTTTCGCCGTAGAGGGATTGGTTGCAACCTTGTCCTGCGTCTCGAGGAATGGTTCGTGGCCAATGATGTAGAGTATACTTATATGGCAACGGAGAAAGACAATGAAGCCTCTGTGAAACTATTCATCTACAAGCTTGGATACACCAACTTTAGAGTTCCGGCGATTTTGGTGAACCCAGTGAAACATTACCGATCATATCACCTCCCTTCCAATATCCAAATCTCTCGTCTTAAAGTCGGGGTTGCCGAGTATCTCTACCGAAAATTCATGGCCTCTACCGAGTTTTTCCCCCACGACATTGATCAAGTTCTCAGACACAAGCTAAGCCTCGGTACATGGGTTGCTTACTATAGAGACGACACCGACGATGCCGAGGACGCCAATTTTGAAACAAGTGGCAGCAAATCAGAAATGACAATACCAAAGAGCTGGGCAATGCTGAGCGTATGGAATAGTGGAGAGGTATTCAAGTTGCGGCTTGGGAAGGCACCATTGTCATGCTTGATATACACAGAGAGCTCAAAGGTGATAGACAAGATCTTCCCATGCCTAAAGTTACCATCAATACCAAATTTCTACGAACCATTCGGATTCTACTTTATGTACGGGGTTCACCGAGAGGGGGCAAGGACAGGGAAGCTGGTGAGGGCATTGTGCCAGTACGTGCACAACGTGGCGGCCACAACAAGGGACTGTAAAGCAATAGTAACAGAGATTGGAGGAGAAGATTCACTAAGAGAGGAGATTCCACATTGGAAATTGCTATCATGCCCTGAAGATTTATGGTGCATAAAGGGATTGAAGAAAGAAGCAAGAAATAGCCTTCACGAGTTGACAAAAACCCCATCAACTACAAGACCAGCCCTTTTTGTAGACCCAAGAGAAGTATGA

Protein sequence

FLCQRRSGERKMGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV
Homology
BLAST of Carg26033 vs. NCBI nr
Match: KAG7019119.1 (putative N-acetyltransferase HLS1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 845.9 bits (2184), Expect = 1.5e-241
Identity = 410/410 (100.00%), Postives = 410/410 (100.00%), Query Frame = 0

Query: 1   FLCQRRSGERKMGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPI 60
           FLCQRRSGERKMGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPI
Sbjct: 1   FLCQRRSGERKMGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPI 60

Query: 61  CRIRNSPLYKMLVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGI 120
           CRIRNSPLYKMLVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGI
Sbjct: 61  CRIRNSPLYKMLVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGI 120

Query: 121 GCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSY 180
           GCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSY
Sbjct: 121 GCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSY 180

Query: 181 HLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAED 240
           HLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAED
Sbjct: 181 HLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAED 240

Query: 241 ANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKL 300
           ANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKL
Sbjct: 241 ANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKL 300

Query: 301 PSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLR 360
           PSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLR
Sbjct: 301 PSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLR 360

Query: 361 EEIPHWKLLSCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           EEIPHWKLLSCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV
Sbjct: 361 EEIPHWKLLSCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 410

BLAST of Carg26033 vs. NCBI nr
Match: KAG6583349.1 (putative N-acetyltransferase HLS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 822.8 bits (2124), Expect = 1.3e-234
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL
Sbjct: 121 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE
Sbjct: 181 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG
Sbjct: 241 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV
Sbjct: 361 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 399

BLAST of Carg26033 vs. NCBI nr
Match: XP_022964994.1 (probable N-acetyltransferase HLS1 [Cucurbita moschata])

HSP 1 Score: 807.4 bits (2084), Expect = 5.8e-230
Identity = 391/399 (97.99%), Postives = 393/399 (98.50%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQAQKD AKVGYVLGLRV PSFRRRGIGC+LVLRLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAQKDWAKVGYVLGLRVVPSFRRRGIGCSLVLRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FVANDV+YTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL
Sbjct: 121 FVANDVDYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE
Sbjct: 181 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG
Sbjct: 241 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREGARTGKLVR LC YVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGARTGKLVRTLCHYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 399

BLAST of Carg26033 vs. NCBI nr
Match: XP_008457342.1 (PREDICTED: probable N-acetyltransferase HLS1 [Cucumis melo] >KAA0060026.1 putative N-acetyltransferase HLS1 [Cucumis melo var. makuwa] >TYJ97283.1 putative N-acetyltransferase HLS1 [Cucumis melo var. makuwa])

HSP 1 Score: 753.1 bits (1943), Expect = 1.3e-213
Identity = 366/399 (91.73%), Postives = 376/399 (94.24%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQSADRGRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIGC+LV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGCSLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FV NDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYRSYHLPSNIQI+RL
Sbjct: 121 FVINDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRSYHLPSNIQIARL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLGTWVAYY+D  DD   A FET+ SKSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGTWVAYYKD--DDVSSAKFETNSSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           +TIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 ITIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREG  TGKLVRALCQYVHN+AA  RDCK IVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGTGTGKLVRALCQYVHNMAAAARDCKVIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 397

BLAST of Carg26033 vs. NCBI nr
Match: XP_011658711.1 (probable N-acetyltransferase HLS1 [Cucumis sativus])

HSP 1 Score: 749.6 bits (1934), Expect = 1.4e-212
Identity = 364/399 (91.23%), Postives = 375/399 (93.98%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQSADRGRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIGC+LV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGCSLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           F+ NDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYRSY LPSNIQI+RL
Sbjct: 121 FMINDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRSYQLPSNIQIARL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLGTWVAYY+D  DD     FET+GSKSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGTWVAYYKD--DDVSSTKFETNGSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           +TIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 ITIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREG  TGKLVRALCQYVHN+AA  RDCK IVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGTGTGKLVRALCQYVHNMAAAARDCKVIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 397

BLAST of Carg26033 vs. ExPASy Swiss-Prot
Match: Q42381 (Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 7.9e-97
Identity = 195/406 (48.03%), Postives = 261/406 (64.29%), Query Frame = 0

Query: 20  IIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAEV--- 79
           ++R YD    D V VED+ERRCEVGPS ++ LFTD +GDPICRIR+SP Y MLVAE+   
Sbjct: 3   VVREYD-PTRDLVGVEDVERRCEVGPSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGTE 62

Query: 80  DNQLVGVIQGSIKVVTV-------HQAQKD-----RAKVGYVLGLRVAPSFRRRGIGCNL 139
             ++VG+I+G IK VT        H++Q D       K+ YVLGLRV+P  RR+GIG  L
Sbjct: 63  KKEIVGMIRGCIKTVTCGQKLDLNHKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGIGFKL 122

Query: 140 VLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPS 199
           V  +EEWF  N  EY+Y+ATE DN+ASV LF  K GY+ FR P+ILVNPV  +R  ++  
Sbjct: 123 VKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHR-VNVSR 182

Query: 200 NIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFE 259
            + + +L+   AE LYR   ++TEFFP DID VL +KLSLGT+VA  R     +   ++ 
Sbjct: 183 RVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSGSWP 242

Query: 260 TSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP 319
            S    E   P+SWA+LSVWN  + F L +  A     +  ++++V+DK  P LKLPSIP
Sbjct: 243 GSAKFLEYP-PESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPSIP 302

Query: 320 NFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIP 379
           + +EPFG +FMYG+  EG R  K+V++LC + HN+ A    C  +  E+ GED LR  IP
Sbjct: 303 SVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNL-AKAGGCGVVAAEVAGEDPLRRGIP 362

Query: 380 HWKLLSCPEDLWCIKGLKKEARNS-LHELTKTPSTTRPALFVDPRE 410
           HWK+LSC EDLWCIK L  +  +  + + TK+P     ++FVDPRE
Sbjct: 363 HWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGV--SIFVDPRE 402

BLAST of Carg26033 vs. ExPASy Swiss-Prot
Match: O64815 (Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23060 PE=2 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 4.8e-94
Identity = 188/412 (45.63%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 21  IRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAEV---- 80
           +R YD    D   VED+ERRCEVGP+ ++ LFTD +GDPICR+R+SP Y MLVAE+    
Sbjct: 7   VREYDPS-KDLATVEDVERRCEVGPAGKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPKE 66

Query: 81  DNQLVGVIQGSIKVVTV-----------HQAQKD-------RAKVGYVLGLRVAPSFRRR 140
             +LVG+I+G IK VT            +++Q D         K+ Y+LGLRV+P+ RR+
Sbjct: 67  KKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSPTHRRQ 126

Query: 141 GIGCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYR 200
           GIG  LV  +E+WF  N  EY+Y ATE DN ASV LF  K GY  FR P+ILVNPV  +R
Sbjct: 127 GIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVYAHR 186

Query: 201 SYHLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDA 260
             ++   + + +L+   AE LYR   ++TEFFP DID VL +KLSLGT+VA  R     +
Sbjct: 187 -VNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGS 246

Query: 261 EDANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCL 320
              ++  S    E   P SWA+LSVWN  + F+L +  A     + +++++++DK  P L
Sbjct: 247 GSRSWPGSAKFLEYP-PDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPFL 306

Query: 321 KLPSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDS 380
           K+PSIP  + PFG +FMYG+  EG R  K+V+ALC + HN+ A    C  +  E+ GE+ 
Sbjct: 307 KIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNL-AKEGGCGVVAAEVAGEEP 366

Query: 381 LREEIPHWKLLSCPEDLWCIKGLKKE-ARNSLHELTKTPSTTRPALFVDPRE 410
           LR  IPHWK+LSC EDLWCIK L ++ +  S+ + TK+P     ++FVDPRE
Sbjct: 367 LRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSP--PGDSIFVDPRE 412

BLAST of Carg26033 vs. ExPASy TrEMBL
Match: A0A6J1HPS7 (probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111464938 PE=4 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 2.8e-230
Identity = 391/399 (97.99%), Postives = 393/399 (98.50%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQAQKD AKVGYVLGLRV PSFRRRGIGC+LVLRLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAQKDWAKVGYVLGLRVVPSFRRRGIGCSLVLRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FVANDV+YTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL
Sbjct: 121 FVANDVDYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE
Sbjct: 181 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG
Sbjct: 241 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREGARTGKLVR LC YVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGARTGKLVRTLCHYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 399

BLAST of Carg26033 vs. ExPASy TrEMBL
Match: A0A5D3BD96 (Putative N-acetyltransferase HLS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold194G00770 PE=4 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.3e-214
Identity = 366/399 (91.73%), Postives = 376/399 (94.24%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQSADRGRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIGC+LV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGCSLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FV NDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYRSYHLPSNIQI+RL
Sbjct: 121 FVINDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRSYHLPSNIQIARL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLGTWVAYY+D  DD   A FET+ SKSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGTWVAYYKD--DDVSSAKFETNSSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           +TIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 ITIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREG  TGKLVRALCQYVHN+AA  RDCK IVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGTGTGKLVRALCQYVHNMAAAARDCKVIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 397

BLAST of Carg26033 vs. ExPASy TrEMBL
Match: A0A1S3C5Y9 (probable N-acetyltransferase HLS1 OS=Cucumis melo OX=3656 GN=LOC103497055 PE=4 SV=1)

HSP 1 Score: 753.1 bits (1943), Expect = 6.3e-214
Identity = 366/399 (91.73%), Postives = 376/399 (94.24%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQSADRGRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIGC+LV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGCSLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FV NDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYRSYHLPSNIQI+RL
Sbjct: 121 FVINDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRSYHLPSNIQIARL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLGTWVAYY+D  DD   A FET+ SKSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGTWVAYYKD--DDVSSAKFETNSSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           +TIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 ITIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREG  TGKLVRALCQYVHN+AA  RDCK IVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGTGTGKLVRALCQYVHNMAAAARDCKVIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 397

BLAST of Carg26033 vs. ExPASy TrEMBL
Match: A0A0A0M0V6 (N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G533380 PE=4 SV=1)

HSP 1 Score: 749.6 bits (1934), Expect = 7.0e-213
Identity = 364/399 (91.23%), Postives = 375/399 (93.98%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MGCEEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MGCEEEILIIRSYDGQSADRGRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIGC+LV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGCSLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           F+ NDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYRSY LPSNIQI+RL
Sbjct: 121 FMINDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRSYQLPSNIQIARL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLGTWVAYY+D  DD     FET+GSKSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGTWVAYYKD--DDVSSTKFETNGSKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           +TIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 ITIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLLSC 371
           FYFMYGVHREG  TGKLVRALCQYVHN+AA  RDCK IVTEIGGEDSLREEIPHWKLLSC
Sbjct: 301 FYFMYGVHREGTGTGKLVRALCQYVHNMAAAARDCKVIVTEIGGEDSLREEIPHWKLLSC 360

Query: 372 PEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           PEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 PEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 397

BLAST of Carg26033 vs. ExPASy TrEMBL
Match: A0A6J1K8Q3 (probable N-acetyltransferase HLS1 OS=Cucurbita maxima OX=3661 GN=LOC111493289 PE=4 SV=1)

HSP 1 Score: 728.0 bits (1878), Expect = 2.2e-206
Identity = 358/401 (89.28%), Postives = 369/401 (92.02%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           M  EEEILIIRSYDGQ ADR RVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM
Sbjct: 1   MRYEEEILIIRSYDGQSADRARVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEW 131
           LVAEVDNQLVGVIQGSIKVVTVHQA KDRAKVGYVLGLRVAPSFRRRGIG NLV RLEEW
Sbjct: 61  LVAEVDNQLVGVIQGSIKVVTVHQAPKDRAKVGYVLGLRVAPSFRRRGIGYNLVRRLEEW 120

Query: 132 FVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRL 191
           FVANDV+Y YMATEKDNEASVKLFI KLGYTNFRVPAILVNPVKHYR YHLPSNIQIS L
Sbjct: 121 FVANDVDYAYMATEKDNEASVKLFINKLGYTNFRVPAILVNPVKHYRPYHLPSNIQISSL 180

Query: 192 KVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSE 251
           KV VAE+LYRKFMASTEFFPHDID VL+HKLSLG+WVAYY+D  DD+    FET+G KSE
Sbjct: 181 KVDVAEFLYRKFMASTEFFPHDIDHVLKHKLSLGSWVAYYKD--DDSTTPKFETNGGKSE 240

Query: 252 MTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFG 311
           M IPK WAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP+FYEPFG
Sbjct: 241 MVIPKCWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPDFYEPFG 300

Query: 312 FYFMYGVHREGARTG--KLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIPHWKLL 371
           FYFMYGVHREG   G  KLV+ALCQYVHN+AA  RDCK IVTEIGGEDSLR+EIPHWKLL
Sbjct: 301 FYFMYGVHREGKGMGTRKLVKALCQYVHNMAAAARDCKVIVTEIGGEDSLRDEIPHWKLL 360

Query: 372 SCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
           SCPEDLWCIK LKKEARNSLHELTKTP TTRPALFVDPREV
Sbjct: 361 SCPEDLWCIKALKKEARNSLHELTKTPPTTRPALFVDPREV 399

BLAST of Carg26033 vs. TAIR 10
Match: AT2G30090.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 403.7 bits (1036), Expect = 1.8e-112
Identity = 216/403 (53.60%), Postives = 276/403 (68.49%), Query Frame = 0

Query: 16  EEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAE 75
           +E ++IR YD +  DR+++  +E+ CE+G   +  LFTDT+GDPICRIRNSP + MLVA 
Sbjct: 10  DEEVVIRCYDDR-RDRIQMGRMEKSCEIGHDHQTLLFTDTLGDPICRIRNSPFFIMLVAG 69

Query: 76  VDNQLVGVIQGSIKVVTVHQAQKDRAKVGYVLGLRVAPSFRRRGIGCNLVLRLEEWFVAN 135
           V N+LVG IQGS+K V  H       +VGYVLGLRV PS+RRRGIG  LV +LEEWF ++
Sbjct: 70  VGNKLVGSIQGSVKPVEFHDKS---VRVGYVLGLRVVPSYRRRGIGSILVRKLEEWFESH 129

Query: 136 DVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPSNIQISRLKVGV 195
           + +Y YMATEKDNEAS  LFI +LGY  FR PAILVNPV   R   LPS+I I +LKV  
Sbjct: 130 NADYAYMATEKDNEASHGLFIGRLGYVVFRNPAILVNPVNPGRGLKLPSDIGIRKLKVKE 189

Query: 196 AEYLYRK-FMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFETSGSKSEMTI 255
           AE LYR+   A+TEFFP DI+++LR+KLS+GTWVAYY ++ D+                 
Sbjct: 190 AESLYRRNVAATTEFFPDDINKILRNKLSIGTWVAYY-NNVDNT---------------- 249

Query: 256 PKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIPNFYEPFGFYF 315
            +SWAMLSVW+S +VFKLR+ +APLS L+ T+ SK+       L L  +P+ + PFGFYF
Sbjct: 250 -RSWAMLSVWDSSKVFKLRIERAPLSYLLLTKVSKLFGNFLSLLGLTVLPDLFTPFGFYF 309

Query: 316 MYGVHREGARTGKLVRALCQYVHNVAATTRD--CKAIVTEI----GGEDSLREEIPHWKL 375
           +YGVH EG   GKLVRALC++VHN+AA      CK +V E+     G+DSL+  IPHWK+
Sbjct: 310 LYGVHSEGPHCGKLVRALCEHVHNMAALNDGCACKVVVVEVDKGSNGDDSLQRCIPHWKM 369

Query: 376 LSCPEDLWCIKGLKKEARN-SLHELTKTPSTTRPALFVDPREV 411
           LSC +D+WCIK LK E     L E +K    +R +LFVDPREV
Sbjct: 370 LSCDDDMWCIKPLKCEKNKFDLSERSK----SRSSLFVDPREV 386

BLAST of Carg26033 vs. TAIR 10
Match: AT4G37580.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 355.5 bits (911), Expect = 5.6e-98
Identity = 195/406 (48.03%), Postives = 261/406 (64.29%), Query Frame = 0

Query: 20  IIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAEV--- 79
           ++R YD    D V VED+ERRCEVGPS ++ LFTD +GDPICRIR+SP Y MLVAE+   
Sbjct: 3   VVREYD-PTRDLVGVEDVERRCEVGPSGKLSLFTDLLGDPICRIRHSPSYLMLVAEMGTE 62

Query: 80  DNQLVGVIQGSIKVVTV-------HQAQKD-----RAKVGYVLGLRVAPSFRRRGIGCNL 139
             ++VG+I+G IK VT        H++Q D       K+ YVLGLRV+P  RR+GIG  L
Sbjct: 63  KKEIVGMIRGCIKTVTCGQKLDLNHKSQNDVVKPLYTKLAYVLGLRVSPFHRRQGIGFKL 122

Query: 140 VLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYHLPS 199
           V  +EEWF  N  EY+Y+ATE DN+ASV LF  K GY+ FR P+ILVNPV  +R  ++  
Sbjct: 123 VKMMEEWFRQNGAEYSYIATENDNQASVNLFTGKCGYSEFRTPSILVNPVYAHR-VNVSR 182

Query: 200 NIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDANFE 259
            + + +L+   AE LYR   ++TEFFP DID VL +KLSLGT+VA  R     +   ++ 
Sbjct: 183 RVTVIKLEPVDAETLYRIRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGSGSGSWP 242

Query: 260 TSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLPSIP 319
            S    E   P+SWA+LSVWN  + F L +  A     +  ++++V+DK  P LKLPSIP
Sbjct: 243 GSAKFLEYP-PESWAVLSVWNCKDSFLLEVRGASRLRRVVAKTTRVVDKTLPFLKLPSIP 302

Query: 320 NFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLREEIP 379
           + +EPFG +FMYG+  EG R  K+V++LC + HN+ A    C  +  E+ GED LR  IP
Sbjct: 303 SVFEPFGLHFMYGIGGEGPRAVKMVKSLCAHAHNL-AKAGGCGVVAAEVAGEDPLRRGIP 362

Query: 380 HWKLLSCPEDLWCIKGLKKEARNS-LHELTKTPSTTRPALFVDPRE 410
           HWK+LSC EDLWCIK L  +  +  + + TK+P     ++FVDPRE
Sbjct: 363 HWKVLSCDEDLWCIKRLGDDYSDGVVGDWTKSPPGV--SIFVDPRE 402

BLAST of Carg26033 vs. TAIR 10
Match: AT2G23060.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 3.4e-95
Identity = 188/412 (45.63%), Postives = 258/412 (62.62%), Query Frame = 0

Query: 21  IRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKMLVAEV---- 80
           +R YD    D   VED+ERRCEVGP+ ++ LFTD +GDPICR+R+SP Y MLVAE+    
Sbjct: 7   VREYDPS-KDLATVEDVERRCEVGPAGKLSLFTDLLGDPICRVRHSPSYLMLVAEIGPKE 66

Query: 81  DNQLVGVIQGSIKVVTV-----------HQAQKD-------RAKVGYVLGLRVAPSFRRR 140
             +LVG+I+G IK VT            +++Q D         K+ Y+LGLRV+P+ RR+
Sbjct: 67  KKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILGLRVSPTHRRQ 126

Query: 141 GIGCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYR 200
           GIG  LV  +E+WF  N  EY+Y ATE DN ASV LF  K GY  FR P+ILVNPV  +R
Sbjct: 127 GIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPSILVNPVYAHR 186

Query: 201 SYHLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDA 260
             ++   + + +L+   AE LYR   ++TEFFP DID VL +KLSLGT+VA  R     +
Sbjct: 187 -VNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFVAVPRGSCYGS 246

Query: 261 EDANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCL 320
              ++  S    E   P SWA+LSVWN  + F+L +  A     + +++++++DK  P L
Sbjct: 247 GSRSWPGSAKFLEYP-PDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKATRMVDKTLPFL 306

Query: 321 KLPSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDS 380
           K+PSIP  + PFG +FMYG+  EG R  K+V+ALC + HN+ A    C  +  E+ GE+ 
Sbjct: 307 KIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNL-AKEGGCGVVAAEVAGEEP 366

Query: 381 LREEIPHWKLLSCPEDLWCIKGLKKE-ARNSLHELTKTPSTTRPALFVDPRE 410
           LR  IPHWK+LSC EDLWCIK L ++ +  S+ + TK+P     ++FVDPRE
Sbjct: 367 LRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSP--PGDSIFVDPRE 412

BLAST of Carg26033 vs. TAIR 10
Match: AT5G67430.1 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 317.8 bits (813), Expect = 1.3e-86
Identity = 178/409 (43.52%), Postives = 254/409 (62.10%), Query Frame = 0

Query: 12  MGCEEEILIIRSYDGQYADRVRVEDLERRCEVGPSERVFLFTDTMGDPICRIRNSPLYKM 71
           MG    ++++R YD +  D   VE+LE  CEVG      L  D MGDP+ RIR SP + M
Sbjct: 1   MGKGFNVVVVREYDPK-RDLTSVEELEESCEVGS-----LLVDLMGDPLARIRQSPSFHM 60

Query: 72  LVAEVDNQLVGVIQGSIKVVT-----VHQA-----QKDRAKVGYVLGLRVAPSFRRRGIG 131
           LVAE+ N++VG+I+G+IK+VT     + QA     + +  K+ +V GLRV+P +RR GIG
Sbjct: 61  LVAEIGNEIVGMIRGTIKMVTRGVNALRQADDVSPEINTTKLAFVSGLRVSPFYRRMGIG 120

Query: 132 CNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPAILVNPVKHYRSYH 191
             LV RLEEWF+ ND  Y+Y+ TE DN ASVKLF  K GY+ FR P  LVNPV ++R   
Sbjct: 121 LKLVQRLEEWFLRNDAVYSYVQTENDNIASVKLFTEKSGYSKFRTPTFLVNPVFNHR-VT 180

Query: 192 LPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWVAYYRDDTDDAEDA 251
           +   ++I +L    AE LYR   ++TEFFP DI+ +L +KLSLGT++A  R   +     
Sbjct: 181 VSRRVKIIKLAPSDAESLYRNRFSTTEFFPSDINSILTNKLSLGTYLAVPRGGDN----- 240

Query: 252 NFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESSKVIDKIFPCLKLP 311
               SGS  + T   SWA++S+WNS +V++L++  A     +  +S++V D  FP LK+P
Sbjct: 241 ---VSGSLPDQT--GSWAVISIWNSKDVYRLQVKGASRLKRMLAKSTRVFDGAFPFLKIP 300

Query: 312 SIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKAIVTEIGGEDSLRE 371
           S PN ++ F  +FMYG+  EG R  ++V ALC + HN+A  +  C  +  E+   + LR 
Sbjct: 301 SFPNLFKSFAMHFMYGIGGEGPRAAEMVEALCSHAHNLARKS-GCAVVAAEVASCEPLRV 360

Query: 372 EIPHWKLLSCPEDLWCIKGLKKEARNSLHELTKTPSTTRPALFVDPREV 411
            IPHWK+LS PEDLWC+K L+ +  +   + TK+P     ++FVDPRE+
Sbjct: 361 GIPHWKVLS-PEDLWCLKRLRYD--DDGVDWTKSPPGL--SIFVDPREI 386

BLAST of Carg26033 vs. TAIR 10
Match: AT2G23060.2 (Acyl-CoA N-acyltransferases (NAT) superfamily protein )

HSP 1 Score: 285.4 bits (729), Expect = 7.1e-77
Identity = 159/362 (43.92%), Postives = 221/362 (61.05%), Query Frame = 0

Query: 71  MLVAEV----DNQLVGVIQGSIKVVTV-----------HQAQKD-------RAKVGYVLG 130
           MLVAE+      +LVG+I+G IK VT            +++Q D         K+ Y+LG
Sbjct: 1   MLVAEIGPKEKKELVGMIRGCIKTVTCGITTKRLDLTHNKSQNDVVITKPLYTKLAYILG 60

Query: 131 LRVAPSFRRRGIGCNLVLRLEEWFVANDVEYTYMATEKDNEASVKLFIYKLGYTNFRVPA 190
           LRV+P+ RR+GIG  LV  +E+WF  N  EY+Y ATE DN ASV LF  K GY  FR P+
Sbjct: 61  LRVSPTHRRQGIGFKLVKAMEDWFSQNGAEYSYFATENDNHASVNLFTGKCGYAEFRTPS 120

Query: 191 ILVNPVKHYRSYHLPSNIQISRLKVGVAEYLYRKFMASTEFFPHDIDQVLRHKLSLGTWV 250
           ILVNPV  +R  ++   + + +L+   AE LYR   ++TEFFP DID VL +KLSLGT+V
Sbjct: 121 ILVNPVYAHR-VNISRRVTVIKLEPSDAELLYRLRFSTTEFFPRDIDSVLNNKLSLGTFV 180

Query: 251 AYYRDDTDDAEDANFETSGSKSEMTIPKSWAMLSVWNSGEVFKLRLGKAPLSCLIYTESS 310
           A  R     +   ++  S    E   P SWA+LSVWN  + F+L +  A     + ++++
Sbjct: 181 AVPRGSCYGSGSRSWPGSAKFLEYP-PDSWAVLSVWNCKDSFRLEVRGASRLRRVVSKAT 240

Query: 311 KVIDKIFPCLKLPSIPNFYEPFGFYFMYGVHREGARTGKLVRALCQYVHNVAATTRDCKA 370
           +++DK  P LK+PSIP  + PFG +FMYG+  EG R  K+V+ALC + HN+ A    C  
Sbjct: 241 RMVDKTLPFLKIPSIPAVFRPFGLHFMYGIGGEGPRAEKMVKALCDHAHNL-AKEGGCGV 300

Query: 371 IVTEIGGEDSLREEIPHWKLLSCPEDLWCIKGLKKE-ARNSLHELTKTPSTTRPALFVDP 410
           +  E+ GE+ LR  IPHWK+LSC EDLWCIK L ++ +  S+ + TK+P     ++FVDP
Sbjct: 301 VAAEVAGEEPLRRGIPHWKVLSCAEDLWCIKRLGEDYSDGSVGDWTKSP--PGDSIFVDP 357

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019119.11.5e-241100.00putative N-acetyltransferase HLS1, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6583349.11.3e-234100.00putative N-acetyltransferase HLS1, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022964994.15.8e-23097.99probable N-acetyltransferase HLS1 [Cucurbita moschata][more]
XP_008457342.11.3e-21391.73PREDICTED: probable N-acetyltransferase HLS1 [Cucumis melo] >KAA0060026.1 putati... [more]
XP_011658711.11.4e-21291.23probable N-acetyltransferase HLS1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q423817.9e-9748.03Probable N-acetyltransferase HLS1 OS=Arabidopsis thaliana OX=3702 GN=HLS1 PE=1 S... [more]
O648154.8e-9445.63Probable N-acetyltransferase HLS1-like OS=Arabidopsis thaliana OX=3702 GN=At2g23... [more]
Match NameE-valueIdentityDescription
A0A6J1HPS72.8e-23097.99probable N-acetyltransferase HLS1 OS=Cucurbita moschata OX=3662 GN=LOC111464938 ... [more]
A0A5D3BD966.3e-21491.73Putative N-acetyltransferase HLS1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A1S3C5Y96.3e-21491.73probable N-acetyltransferase HLS1 OS=Cucumis melo OX=3656 GN=LOC103497055 PE=4 S... [more]
A0A0A0M0V67.0e-21391.23N-acetyltransferase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
A0A6J1K8Q32.2e-20689.28probable N-acetyltransferase HLS1 OS=Cucurbita maxima OX=3661 GN=LOC111493289 PE... [more]
Match NameE-valueIdentityDescription
AT2G30090.11.8e-11253.60Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT4G37580.15.6e-9848.03Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.13.4e-9545.63Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT5G67430.11.3e-8643.52Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
AT2G23060.27.1e-7743.92Acyl-CoA N-acyltransferases (NAT) superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000182GNAT domainPFAMPF00583Acetyltransf_1coord: 37..161
e-value: 4.0E-14
score: 52.8
IPR000182GNAT domainPROSITEPS51186GNATcoord: 19..205
score: 15.721779
NoneNo IPR availableGENE3D3.40.630.30coord: 44..167
e-value: 3.9E-17
score: 64.6
NoneNo IPR availablePANTHERPTHR47370ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEINcoord: 17..410
NoneNo IPR availablePANTHERPTHR47370:SF1ACYL-COA N-ACYLTRANSFERASES (NAT) SUPERFAMILY PROTEINcoord: 17..410
NoneNo IPR availableCDDcd04301NAT_SFcoord: 71..139
e-value: 2.52099E-7
score: 45.7297
IPR016181Acyl-CoA N-acyltransferaseSUPERFAMILY55729Acyl-CoA N-acyltransferases (Nat)coord: 49..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg26033-RACarg26033-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0008080 N-acetyltransferase activity