Carg26004 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg26004
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	(1->3)-beta-glucan endohydrolase
Location	Carg_Chr12: 11505738 .. 11508305 (-)
RNA-Seq Expression	Carg26004
Synteny	Carg26004

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAGAAGCAAAAGAATGTCGAAGTTTGAATTACAAATCCACAGGAATTTCATACTCAGAACAATTCTTTTGTTGTTCTTGACGATTATTCCAAACGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCGACTCACCAGCTCCCACCAGAGAAGGTTGTTAAAATGCTTCAAGAAAATGGGTTTCTTAAATTGAAGCTCTTTGAAGCTGAGGATAGGATTTTGGAAGCGCTTATTGGAACTGATATTGAAGTTATGTTGGCTCTTCCCAATTCAATGCTGCAAATTATGAGTGAGGATTCTAAGGCTGCTGCTTATTGGGTGGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTTAATATCAAGTTGAGTATTGAAGATTCTTGATCTTACATTACGAACTTTGCATTTTGTGTTTTATTTTGTGCACTTTAGGTGTTTGATGAAATGCCCACTTCATGATTTCCTCTGTTTCTGCTGCTGCTTTCATTTTAGATCGATTCATTGAATACCAATCTGTTTCTTTCTCCATTTTCTTTTGTTTAGTTCTATATTTGTGCGAGATCTCGTTTCGGTTGCGGAGGAGAATGAAACATTCTTTATAAGGGTGTGGAAACCTTTCCATAGCATATGTGTTTTAAAAACCTTGAGAGGAAGCCCAAAGAGAACAATATTTGCTAGCGGTAGGCTTGAGATGTTGCAAATGGTATCAGAGCCAAACATCAGGCGATGTGCCAACGAAGAGGCTTAGCTTCAAAGGGGGTGGACACGAGGCGGTGTGCCAGCAAGGATGCTGAGCCCCGAAGGGGGTGGATTGTGGGGTCCCACGTCGATTGAAGAAGGGAATGAGTGCCAGCGAGGATGCTAGCCTTGAAGGGAAGTGGGTTGTGAGATCCCACATCGGTCGGGGAGGAGAATGAAACATTCTTTATAAGGGTGTGGAAACCTCTCTCTTAGTAGACGCGATTTAAAAACCTTGAGGGAAAACTCAAAGAGGAATAATGTTTGCTAGCGGTGGGCTTGGGCTGTTACAAATGGCATCAGAGCCAGACACAAAGCAATGTGCTAGCGAGGAGGCTTAAACCAAAAGGGGAGTGGACACGAGGCGGTGTGCTAGCAAGGATGCTGGGCCCCAAAGAGGGATGGATTGGGGGGTCCCACATTGATTGGAGAAGGGGACGAGCACCAGCCTGAAGGAAGTAGATTGCGAGATCACACATTGGTTGGGGAGGAGAACGAAACATTCTTTATAAAGGTGTGGAAACCTCTCCCTAGCAAACACGTTTTTAAAATCTTGAAGGGAAGTTCGAAAAGGAAAGCCCAAAGAAGACATTATCTACTAGCGGTGAGTTTGGGCTACAACGTTCAAAAGGTTGAACGATGAAAGAGTTGAGATTTGTTAATGCATTTTATGAAGGGTGAGGCATAAAATTCAATTGAAAACTTGTTTTGCAGGTATGTTGCAGTAGGCAATGAGCCCTTTCTGAAGTCCTACAATGGCACTTATCTTCAGTTAACATTGCCTGCCCTGAGGAATATCCAGCAAGCCTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCCGAAGTCCGAGACTTAACGATCGAGATTCTACACTTCCTATCCATAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCTATAGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGATGCCAATTTCGACACGCTCGTTTCGGCTCTAACGAAAGCAGGATATCCTGACATGAAGATCATAGTAGGGGAAATCGGGTGGCCAACGGACGGCGACAAACACGCCAATATCCAAGACGCCAAACGATTCAACCAGGGATTGTTGAGACATGCTCTAAGTGGGGAGGGAACACCAGCTCGAGTCGGCGTAATTGACGTCTATCTCTTCAGCCTGATCGATGAAAATGCCAAAAGCATAGAGCCAGGAAGCTTTGAAAGGCATTGGGGAATCTTTGAATTCGATGGTAAGCCAAAATATGAACTAGATCTGTCTGGAACAGAAGAAGAAAAGGGGTTAATAGCAGTAGAAGGAGTGAGATATATGGATAAAAAATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGATTGGCTGATAACATTGATTATGCTTGTAGCTTATCAGATTGCACTGCTTTGGAATATGGATCTTCATGCAATCAATTAACTGCTGAAGGAAATGCTTCTTATGCGTTTAATATGTACTTTCAAATGAACAGCCAAAAGAGATGGAACTGTGAGTTTTCTGGTTTGGCTATGGTGACTGAACAAGATCCTTCTTATGGGAACTGCCAGTTCCCTATTATGATCGATTATAACTCTTCTTCTTCTTCATTTCTAGATCTTCTGGTTGCAATCTTTATGGGATTTGTGGTGTTTTTGAGTTTGGAATGA

mRNA sequence

AAAGAAGCAAAAGAATGTCGAAGTTTGAATTACAAATCCACAGGAATTTCATACTCAGAACAATTCTTTTGTTGTTCTTGACGATTATTCCAAACGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCGACTCACCAGCTCCCACCAGAGAAGGTTGTTAAAATGCTTCAAGAAAATGGGTTTCTTAAATTGAAGCTCTTTGAAGCTGAGGATAGGATTTTGGAAGCGCTTATTGGAACTGATATTGAAGTTATGTTGGCTCTTCCCAATTCAATGCTGCAAATTATGAGTGAGGATTCTAAGGCTGCTGCTTATTGGGTGGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTTAATATCAAGTATGTTGCAGTAGGCAATGAGCCCTTTCTGAAGTCCTACAATGGCACTTATCTTCAGTTAACATTGCCTGCCCTGAGGAATATCCAGCAAGCCTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCCGAAGTCCGAGACTTAACGATCGAGATTCTACACTTCCTATCCATAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCTATAGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGATGCCAATTTCGACACGCTCGTTTCGGCTCTAACGAAAGCAGGATATCCTGACATGAAGATCATAGTAGGGGAAATCGGGTGGCCAACGGACGGCGACAAACACGCCAATATCCAAGACGCCAAACGATTCAACCAGGGATTGTTGAGACATGCTCTAAGTGGGGAGGGAACACCAGCTCGAGTCGGCGTAATTGACGTCTATCTCTTCAGCCTGATCGATGAAAATGCCAAAAGCATAGAGCCAGGAAGCTTTGAAAGGCATTGGGGAATCTTTGAATTCGATGGTAAGCCAAAATATGAACTAGATCTGTCTGGAACAGAAGAAGAAAAGGGGTTAATAGCAGTAGAAGGAGTGAGATATATGGATAAAAAATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGATTGGCTGATAACATTGATTATGCTTGTAGCTTATCAGATTGCACTGCTTTGGAATATGGATCTTCATGCAATCAATTAACTGCTGAAGGAAATGCTTCTTATGCGTTTAATATGTACTTTCAAATGAACAGCCAAAAGAGATGGAACTGTGAGTTTTCTGGTTTGGCTATGGTGACTGAACAAGATCCTTCTTATGGGAACTGCCAGTTCCCTATTATGATCGATTATAACTCTTCTTCTTCTTCATTTCTAGATCTTCTGGTTGCAATCTTTATGGGATTTGTGGTGTTTTTGAGTTTGGAATGA

Coding sequence (CDS)

ATGTCGAAGTTTGAATTACAAATCCACAGGAATTTCATACTCAGAACAATTCTTTTGTTGTTCTTGACGATTATTCCAAACGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCGACTCACCAGCTCCCACCAGAGAAGGTTGTTAAAATGCTTCAAGAAAATGGGTTTCTTAAATTGAAGCTCTTTGAAGCTGAGGATAGGATTTTGGAAGCGCTTATTGGAACTGATATTGAAGTTATGTTGGCTCTTCCCAATTCAATGCTGCAAATTATGAGTGAGGATTCTAAGGCTGCTGCTTATTGGGTGGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTTAATATCAAGTATGTTGCAGTAGGCAATGAGCCCTTTCTGAAGTCCTACAATGGCACTTATCTTCAGTTAACATTGCCTGCCCTGAGGAATATCCAGCAAGCCTTAAATGATGCAGGGCTTGGATCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGATTTTCGGCCCGAAGTCCGAGACTTAACGATCGAGATTCTACACTTCCTATCCATAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCTATAGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGATGCCAATTTCGACACGCTCGTTTCGGCTCTAACGAAAGCAGGATATCCTGACATGAAGATCATAGTAGGGGAAATCGGGTGGCCAACGGACGGCGACAAACACGCCAATATCCAAGACGCCAAACGATTCAACCAGGGATTGTTGAGACATGCTCTAAGTGGGGAGGGAACACCAGCTCGAGTCGGCGTAATTGACGTCTATCTCTTCAGCCTGATCGATGAAAATGCCAAAAGCATAGAGCCAGGAAGCTTTGAAAGGCATTGGGGAATCTTTGAATTCGATGGTAAGCCAAAATATGAACTAGATCTGTCTGGAACAGAAGAAGAAAAGGGGTTAATAGCAGTAGAAGGAGTGAGATATATGGATAAAAAATGGTGTGTTTTGAACCCAAATGTGAAGGATTGGGAAGGATTGGCTGATAACATTGATTATGCTTGTAGCTTATCAGATTGCACTGCTTTGGAATATGGATCTTCATGCAATCAATTAACTGCTGAAGGAAATGCTTCTTATGCGTTTAATATGTACTTTCAAATGAACAGCCAAAAGAGATGGAACTGTGAGTTTTCTGGTTTGGCTATGGTGACTGAACAAGATCCTTCTTATGGGAACTGCCAGTTCCCTATTATGATCGATTATAACTCTTCTTCTTCTTCATTTCTAGATCTTCTGGTTGCAATCTTTATGGGATTTGTGGTGTTTTTGAGTTTGGAATGA

Protein sequence

MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGFVVFLSLE

Homology

[Close]

BLAST of Carg26004 vs. NCBI nr
Match: KAG7021271.1 (Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 992.3 bits (2564), Expect = 1.5e-285
Identity = 487/487 (100.00%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFLSLE
Sbjct: 481 VVFLSLE 487

[Close]

BLAST of Carg26004 vs. NCBI nr
Match: XP_022937442.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata])

HSP 1 Score: 989.9 bits (2558), Expect = 7.5e-285
Identity = 485/487 (99.59%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMS+DSKAAAYWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFLSLE
Sbjct: 481 VVFLSLE 487

[Close]

BLAST of Carg26004 vs. NCBI nr
Match: XP_023537244.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 988.0 bits (2553), Expect = 2.8e-284
Identity = 484/487 (99.38%), Postives = 486/487 (99.79%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGV+YMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFL LE
Sbjct: 481 VVFLCLE 487

[Close]

BLAST of Carg26004 vs. NCBI nr
Match: XP_022965692.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima])

HSP 1 Score: 969.1 bits (2504), Expect = 1.4e-278
Identity = 475/487 (97.54%), Postives = 480/487 (98.56%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSK  LQIHRNF  RTILLLFLTIIPNGE+VGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMS+DSKAAA WVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFF GTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGV+YMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMY+QMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFL LE
Sbjct: 481 VVFLCLE 487

[Close]

BLAST of Carg26004 vs. NCBI nr
Match: KAG6586415.1 (Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916.0 bits (2366), Expect = 1.4e-262
Identity = 448/450 (99.56%), Postives = 449/450 (99.78%), Query Frame = 0


Query: 38  MATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSK 97
           MATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSK
Sbjct: 1   MATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSK 60

Query: 98  AAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 157
           AAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG
Sbjct: 61  AAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLG 120

Query: 158 SKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSL 217
           SKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSL
Sbjct: 121 SKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSL 180

Query: 218 YGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWP 277
           YGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWP
Sbjct: 181 YGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWP 240

Query: 278 TDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHW 337
           TDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHW
Sbjct: 241 TDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHW 300

Query: 338 GIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSL 397
           GIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSL
Sbjct: 301 GIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSL 360

Query: 398 SDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFP 457
           SDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFP
Sbjct: 361 SDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFP 420

Query: 458 IMIDYNSSSSSFLDLLVAIFMGFVVFLSLE 488
           IMIDYNSSSSSFLDLLVAIFMGFVVFL L+
Sbjct: 421 IMIDYNSSSSSFLDLLVAIFMGFVVFLCLK 450

[Close]

BLAST of Carg26004 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 552.0 bits (1421), Expect = 6.8e-156
Identity = 269/451 (59.65%), Postives = 335/451 (74.28%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           I++  L I+ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+
Sbjct: 13  IVIGHLGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 72

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            +EVM+A+PN  L++M+   +A   WV  NVT Y + GGVNI +VAVGNEPFLKSYNG++
Sbjct: 73  GLEVMVAIPNDQLKVMTSYDRAKD-WVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSF 132

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
           + LT PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRP++     +I
Sbjct: 133 INLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQI 192

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FL  NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLV
Sbjct: 193 VDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLV 252

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
           S+L   G+ DM IIVGE+GWPT+GDKHAN   A RF  GLL    + +GTP R   I+VY
Sbjct: 253 SSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVY 312

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LF L+DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  KWC 
Sbjct: 313 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 372

Query: 377 LNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNC 436
            NP  KD   LA NIDYAC+ SDCTAL YGSSCN L A GNASYAFNM+FQ+ +Q    C
Sbjct: 373 FNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESAC 432

Query: 437 EFSGLAMVTEQDPSYGNCQFPIMIDYNSSSS 468
            F GLA +T Q+ S G C FPI I  +S+SS
Sbjct: 433 YFQGLATITTQNISQGQCNFPIQIVASSASS 459

[Close]

BLAST of Carg26004 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 504.6 bits (1298), Expect = 1.3e-141
Identity = 248/446 (55.61%), Postives = 313/446 (70.18%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           +LL    +     S+G NWGT A+H LPP+ VV+ML+ENG  K+KLF+AE   L AL  +
Sbjct: 8   LLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKS 67

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            IEVM+ +PN ML  ++   KAA  WV  NV+ +     VNI+YVAVGNEPFL +YNG+Y
Sbjct: 68  GIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSY 127

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
           L  T PALRNIQ A+  AGL ++VK T P NAD+Y+S  S   PS GDFR  +RDL I I
Sbjct: 128 LSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITI 187

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FLS N  PFTVNIYP++SLY N  FP+D+AFFDG  +P+ DG   Y N+FDAN+DTLV
Sbjct: 188 VKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTLV 247

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
            AL K G+ +M II+GEIGWPTDGD +AN+  AK+FNQG + H   G+GTP R G ID Y
Sbjct: 248 HALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAY 307

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LFSLIDE+AKS++PG FERHWGIF FDG PKY L+L GT     LI  +GVRY+++KWCV
Sbjct: 308 LFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCV 367

Query: 377 LNPNVK-DWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWN 436
           + PNV+ D   +A  + YACSL DCT+L  G+SC  L  + N SYAFN Y+Q+  Q    
Sbjct: 368 MKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTA 427

Query: 437 CEFSGLAMVTEQDPSYGNCQFPIMID 462
           C+F  ++ VT+ DPS G C+FPIMI+
Sbjct: 428 CKFPNISEVTKTDPSTGTCRFPIMIE 450

[Close]

BLAST of Carg26004 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 493.4 bits (1269), Expect = 2.9e-138
Identity = 241/471 (51.17%), Postives = 329/471 (69.85%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           I LL+ +++   E +GVNWG+ A H LPP  VV++L+ENG  K+KLFEA+  IL+AL  T
Sbjct: 13  ITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKALSRT 72

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            I+VM+ +PN +L  ++    AA  WV  NV+A+    GV+I+YVAVGNEPFLK++NGT+
Sbjct: 73  GIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAFNGTF 132

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
             +TLPAL+NIQ A+  AGL ++VK T+P NAD+Y S  ++ +PS GDFRPE+RDL + I
Sbjct: 133 EGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQS--ASNLPSDGDFRPEIRDLMLNI 192

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FLS N APFT+NIYPF+SLY +  FP++FAFFDGT  PI D    Y NV DAN+DTLV
Sbjct: 193 VKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANYDTLV 252

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
            +L K G+ ++ IIVGE+GWPTDGDK+AN+  A+R+NQG +    + +GTP R G +D Y
Sbjct: 253 WSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGAMDAY 312

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LF LIDE+AKSI+PG+FERHWGIF  DG+PKY+L L       GLI  + V Y+ KKWC+
Sbjct: 313 LFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLG---SGNGLIPAKDVHYLAKKWCI 372

Query: 377 LNPNVKDWE-GLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWN 436
           L PN    +  L  ++ YAC  +DCT+L YGSSC  L    N SYAFN Y+Q+++Q    
Sbjct: 373 LAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQLDSA 432

Query: 437 CEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGFVVFLSL 487
           C+F GL++V+ +DPS G+C+F IMI    +S +   + +      ++ LS+
Sbjct: 433 CKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMMPITRSTAVLLLLSI 478

[Close]

BLAST of Carg26004 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.3e-98
Identity = 188/440 (42.73%), Postives = 272/440 (61.82%), Query Frame = 0


Query: 30  SVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSML 89
           +VG+NWGT A+H LPP KVV++L+ NG +K+KLF+A+ ++L AL G++I V + + NSML
Sbjct: 25  AVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGVTIGIQNSML 84

Query: 90  QIMSEDSKAAAYWVDANVTAYTYHGG--VNIKYVAVGNEPFLKSYNGTYLQLTLPALRNI 149
           + ++   K A  WV  NVT Y ++GG  V I+YVAVG EPFL+SY   Y    + A  NI
Sbjct: 85  KSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAMNI 144

Query: 150 QQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPF 209
           Q AL  A L ++VK  +P + D + S      PS+G FR ++    IE+L FL+ +++PF
Sbjct: 145 QNALVKANLANEVKVVVPSSFDSFLSESGR--PSSGHFRADLNKTMIELLSFLTKHHSPF 204

Query: 210 TVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDM 269
            V I PFLS + N    +DF+ F  T K  KDG  TY N FD ++DTLVSAL   G+ ++
Sbjct: 205 FVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYDTLVSALFTIGFSEV 264

Query: 270 KIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKS 329
            I+V +IGWPTDG ++A    A+ F +GL+ H      +  R  V + Y+ SL+DE+ ++
Sbjct: 265 DIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRPPV-ETYIESLLDEDQRN 324

Query: 330 IEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGL 389
           +  G+FERHWG+F FDG+ KY    +     K  +  + V+Y+  KWCV+N N KD    
Sbjct: 325 LSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNVQYLPPKWCVVNNN-KDLSNA 384

Query: 390 ADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQ 449
           +     AC+++DCT++  G SC+ +   GN SYAFN  +Q N     +C F GL ++T  
Sbjct: 385 SARALEACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLITTV 444

Query: 450 DPSYGNCQFPIMIDYNSSSS 468
           DPS  NC+F I +D + SSS
Sbjct: 445 DPSEDNCRFSIQLDTSHSSS 455

[Close]

BLAST of Carg26004 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 238.8 bits (608), Expect = 1.3e-61
Identity = 157/458 (34.28%), Postives = 246/458 (53.71%), Query Frame = 0


Query: 18  LLLFLTIIPNGES---VGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALI 77
           LL+FL+  P+  +   +GVN+G +A +  PP + VK+LQ     K++L+ A+  I++AL 
Sbjct: 10  LLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALA 69

Query: 78  GTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNG 137
           GT + +++   N  +  ++ D  AA  W+++NV    ++    I  + VGNE  L S + 
Sbjct: 70  GTGVGIVIGAANGDVPSLASDPNAATQWINSNV--LPFYPASKIMLITVGNE-ILMSNDP 129

Query: 138 TYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTI 197
             +   LPA++N+Q+AL    LG K+K +   +  +  S D    PS+G F    +    
Sbjct: 130 NLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDP---PSSGSFAAGYQTGLK 189

Query: 198 EILHFLSINNAPFTVNIYPFLSLYGNDYFP--IDFAFFDGTYKPI--KDGDLTYTNVFDA 257
            IL FLS   +PF +N YPF + Y +D  P  + F  F+     +  K G + YTN+FDA
Sbjct: 190 GILQFLSDTGSPFAINPYPFFA-YQSDPRPETLAFCLFEPNAGRVDSKTG-IKYTNMFDA 249

Query: 258 NFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKH---ANIQDAKRFNQGLLRHALSGEGTP 317
             D + SAL   G+  ++I+V E GW + GD +   A++ +AK +N  L+ H  S  GTP
Sbjct: 250 QVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTP 309

Query: 318 ARVG-VIDVYLFSLIDENAKSIEPG-SFERHWGIFEFDGKPKYELDL----SGTEEEKGL 377
              G  +D Y+F+L DEN K   PG S ER +G+F+ D    Y++ L    S ++   G 
Sbjct: 310 LMPGKPVDTYIFALYDENLK---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSGK 369

Query: 378 IAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLS-DCTALEYGSSC-NQLTAEGNAS 437
           +   G       WCV      + E L  ++D+AC    DC A++ G +C        +A+
Sbjct: 370 VTSSG-------WCVPKKGATN-EELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHAA 429

Query: 438 YAFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFP 458
           YA NMYFQ + ++  +C+FS  A VT Q+PSY NC +P
Sbjct: 430 YAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

[Close]

BLAST of Carg26004 vs. ExPASy TrEMBL
Match: A0A6J1FB83 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 PE=3 SV=1)

HSP 1 Score: 989.9 bits (2558), Expect = 3.6e-285
Identity = 485/487 (99.59%), Postives = 487/487 (100.00%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMS+DSKAAAYWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFLSLE
Sbjct: 481 VVFLSLE 487

[Close]

BLAST of Carg26004 vs. ExPASy TrEMBL
Match: A0A6J1HL09 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=3 SV=1)

HSP 1 Score: 969.1 bits (2504), Expect = 6.6e-279
Identity = 475/487 (97.54%), Postives = 480/487 (98.56%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSK  LQIHRNF  RTILLLFLTIIPNGE+VGVNWGTMATHQLPPEKVVKMLQENGFLKL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLALPNSMLQIMS+DSKAAA WVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFF GTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGV+YMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480
           AFNMY+QMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGF 480

Query: 481 VVFLSLE 488
           VVFL LE
Sbjct: 481 VVFLCLE 487

[Close]

BLAST of Carg26004 vs. ExPASy TrEMBL
Match: A0A6J1G3H9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 PE=3 SV=1)

HSP 1 Score: 892.1 bits (2304), Expect = 1.0e-255
Identity = 432/494 (87.45%), Postives = 464/494 (93.93%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MS+  L+ HRN+I   ++LL LT++PNG SVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQ+MSED +AAA WVD+NVTAYTYHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYK IKDG
Sbjct: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDLSG E +KG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGVRYM K+WCVLNPNVKDWEGLA ++DYACSLSDCTALEYGSSCNQL+AEGNASY
Sbjct: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDY-------NSSSSSFLDLL 480
           AFNMY+QMNSQK WNC+FSG+A+VT++DPSYG+CQFP+MIDY       +SSSSSFLDLL
Sbjct: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLL 480

Query: 481 VAIFMGFVVFLSLE 488
           VA FMGF+VFL LE
Sbjct: 481 VAGFMGFLVFLFLE 494

[Close]

BLAST of Carg26004 vs. ExPASy TrEMBL
Match: A0A6J1KB85 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493909 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 8.7e-255
Identity = 430/492 (87.40%), Postives = 461/492 (93.70%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MS+  L+ HRN I R ++LL LT++PNG  VGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSESVLRFHRNCIFRALILLLLTVVPNGLGVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQ+MSED +AAA WVD+NVTAYTYHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYK IKDG
Sbjct: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSALTKAGYP+MKIIVGE+GWPTDGDKHANIQ+AK+FNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPNMKIIVGEVGWPTDGDKHANIQNAKQFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSG E +KG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGIEAQKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           LIAVEGVRYM K+WCVLNPNVKDWEGLA ++DYACSLSDCTALEYGSSCNQL+AEGNASY
Sbjct: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYN-----SSSSSFLDLLVA 480
           AFNMY+QMNSQK WNC+FSG+A+VT++DPSYG+CQFP+MIDY      SS SSFLDLLVA
Sbjct: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSCSSCSSFLDLLVA 480

Query: 481 IFMGFVVFLSLE 488
            FMGF+VFL LE
Sbjct: 481 GFMGFLVFLFLE 492

[Close]

BLAST of Carg26004 vs. ExPASy TrEMBL
Match: A0A6J1DUS6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 PE=3 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 3.3e-254
Identity = 426/489 (87.12%), Postives = 458/489 (93.66%), Query Frame = 0


Query: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60
           MSK  LQIHR+ I   ++LL+ TI PNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIG+DIEVMLA+PNSMLQIMSED +AAA WVD+NVT Y+YHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240
           SAG+FR +VRDLTIEILHFLS NNAPFTVNIYPFLSLY NDYFP+DFAFFDGTYKPI+DG
Sbjct: 181 SAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSLYSNDYFPLDFAFFDGTYKPIRDG 240

Query: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300
           DL YTNVFDANFDTLVSALTKAGYP+MKIIVGE+GWPTDGDKHANIQ AKRFNQGLLRHA
Sbjct: 241 DLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGWPTDGDKHANIQYAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWG+FEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGLFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420
           L+AVEGVRYM K+WCVLNPN+ DWEGLADN+DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACSLSDCTALEYGSSCNQLTAQGNASY 420

Query: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480
           AFNMY+Q+NSQK WNC+FSGLA+VTE+DPSYGNCQFP+MIDY SSSSS       FLDLL
Sbjct: 421 AFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQFPVMIDYTSSSSSPFLVDERFLDLL 480

Query: 481 VAIFMGFVV 483
           VA+ MG +V
Sbjct: 481 VAVIMGLLV 489

[Close]

BLAST of Carg26004 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 647.5 bits (1669), Expect = 8.5e-186
Identity = 305/464 (65.73%), Postives = 378/464 (81.47%), Query Frame = 0


Query: 11  NFILRTILLLFLTII-------PNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLF 70
           +F    I+L +  II        N  +VGVNWG MA+HQLPPEKVVKML +N F KLKLF
Sbjct: 12  HFYNHQIILCYFLIISQVSIASSNTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLF 71

Query: 71  EAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAV 130
           EA+  IL+ALIG+DIEVM+ +PN  L+ M++D+  AA WV+ NVTAY+Y+GGVNIKY+AV
Sbjct: 72  EADQNILDALIGSDIEVMIGIPNRFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAV 131

Query: 131 GNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAG 190
           GNEPFL++YNGTY++ TLPAL NIQ+AL +A L   VK T+PFNADIY SP++NPVPSAG
Sbjct: 132 GNEPFLQTYNGTYVEFTLPALINIQRALEEADL-KNVKVTVPFNADIYFSPEANPVPSAG 191

Query: 191 DFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLT 250
           DFRPE+RD TIEI++FL  +++PFTVNIYPFLSLYGN YFP+DFAFFDGT K ++DG+L 
Sbjct: 192 DFRPELRDATIEIINFLYSHDSPFTVNIYPFLSLYGNAYFPLDFAFFDGTNKSLRDGNLV 251

Query: 251 YTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSG 310
           YTNVFDAN DTL+ A+ +  +  MKIIVGE+GWPTDGDK+AN++ AKRFNQG+++HA+SG
Sbjct: 252 YTNVFDANLDTLICAMERYSFLGMKIIVGEVGWPTDGDKNANVKSAKRFNQGMVKHAMSG 311

Query: 311 EGTPARVGVI-DVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLI 370
            GTPAR GVI DVYLFSL+DE+AKSI PG+FERHWGIFEFDG+PKYELDLSG   +K L+
Sbjct: 312 NGTPARKGVIMDVYLFSLVDEDAKSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLV 371

Query: 371 AVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAF 430
            VE V+Y+ K WC+L+PN  + + L DNIDYACSLSDCTAL YGSSCN LTA GN SYAF
Sbjct: 372 PVEDVKYLPKTWCILDPNAYNLDDLPDNIDYACSLSDCTALGYGSSCNHLTATGNVSYAF 431

Query: 431 NMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSS 467
           NMY+QM+ QK W+C+F GL ++T++DPS   C+FP+MID   S+
Sbjct: 432 NMYYQMHDQKTWDCDFLGLGLITDEDPSDELCEFPVMIDTGDST 474

[Close]

BLAST of Carg26004 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 556.6 bits (1433), Expect = 2.0e-158
Identity = 273/457 (59.74%), Postives = 335/457 (73.30%), Query Frame = 0


Query: 29  ESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGTDIEVMLALPNSM 88
           + +GVNWGTMATH+LPP+KVV+ML++N   K+KLF+A++  + AL G+ +EVM+A+PN  
Sbjct: 19  DGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQ 78

Query: 89  LQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQ 148
           L++M    +A   WV  NVT Y ++GGVNI +VAVGNEPFLKSYNG+++ LT PAL+NIQ
Sbjct: 79  LKVMGSYDRAKD-WVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQ 138

Query: 149 QALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEILHFLSINNAPFT 208
            ALN+AGLGS VKAT+P NAD+Y+SP SNPVPSAG FRP++     +I+ FL  N+AP T
Sbjct: 139 NALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPIT 198

Query: 209 VNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALTKAGYPDMK 268
           +NIYPFLSLYGND FP+++AFFDG  KP+ D  + YTNVFDANFDTLVSAL   G+ DM 
Sbjct: 199 INIYPFLSLYGNDDFPLNYAFFDGA-KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMP 258

Query: 269 IIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVYLFSLIDENAKSI 328
           IIVGE+GWPT+GDKHAN   A RF  GLL       GTP R   I+VYLF L+DE+AKSI
Sbjct: 259 IIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSI 318

Query: 329 EPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCVLNPNVKDWEGLA 388
            PG FERHWGIF+FDG+PK+ +DLSG  + K LI  E V Y  KKWC+ N   KD   LA
Sbjct: 319 APGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLA 378

Query: 389 DNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNCEFSGLAMVTEQD 448
            NIDYAC+ SDCTAL YGSSCN L A GNASYAFNMYFQ+ +Q    C F GLA +T ++
Sbjct: 379 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKN 438

Query: 449 PSYGNCQFPIMIDYNSSSSSFLDLLVAIFMGFVVFLS 486
            S G C FPI I   S++SSF   LV +  G    LS
Sbjct: 439 ISQGQCNFPIQI-VASTASSFSSSLVLLIAGVWFLLS 472

[Close]

BLAST of Carg26004 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 552.0 bits (1421), Expect = 4.9e-157
Identity = 269/451 (59.65%), Postives = 335/451 (74.28%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           I++  L I+ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+
Sbjct: 13  IVIGHLGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 72

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            +EVM+A+PN  L++M+   +A   WV  NVT Y + GGVNI +VAVGNEPFLKSYNG++
Sbjct: 73  GLEVMVAIPNDQLKVMTSYDRAKD-WVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSF 132

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
           + LT PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRP++     +I
Sbjct: 133 INLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQI 192

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FL  NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLV
Sbjct: 193 VDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLV 252

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
           S+L   G+ DM IIVGE+GWPT+GDKHAN   A RF  GLL    + +GTP R   I+VY
Sbjct: 253 SSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVY 312

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LF L+DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  KWC 
Sbjct: 313 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 372

Query: 377 LNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNC 436
            NP  KD   LA NIDYAC+ SDCTAL YGSSCN L A GNASYAFNM+FQ+ +Q    C
Sbjct: 373 FNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESAC 432

Query: 437 EFSGLAMVTEQDPSYGNCQFPIMIDYNSSSS 468
            F GLA +T Q+ S G C FPI I  +S+SS
Sbjct: 433 YFQGLATITTQNISQGQCNFPIQIVASSASS 459

[Close]

BLAST of Carg26004 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 552.0 bits (1421), Expect = 4.9e-157
Identity = 269/451 (59.65%), Postives = 335/451 (74.28%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           I++  L I+ NG  +GVNWGTMATH+LPP+ VV+ML++N   K+KLF+A++  + AL G+
Sbjct: 13  IVIGHLGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGS 72

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            +EVM+A+PN  L++M+   +A   WV  NVT Y + GGVNI +VAVGNEPFLKSYNG++
Sbjct: 73  GLEVMVAIPNDQLKVMTSYDRAKD-WVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSF 132

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
           + LT PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRP++     +I
Sbjct: 133 INLTFPALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQI 192

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FL  NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLV
Sbjct: 193 VDFLGKNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLV 252

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
           S+L   G+ DM IIVGE+GWPT+GDKHAN   A RF  GLL    + +GTP R   I+VY
Sbjct: 253 SSLKAVGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVY 312

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LF L+DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  KWC 
Sbjct: 313 LFGLLDEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCT 372

Query: 377 LNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWNC 436
            NP  KD   LA NIDYAC+ SDCTAL YGSSCN L A GNASYAFNM+FQ+ +Q    C
Sbjct: 373 FNPEAKDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESAC 432

Query: 437 EFSGLAMVTEQDPSYGNCQFPIMIDYNSSSS 468
            F GLA +T Q+ S G C FPI I  +S+SS
Sbjct: 433 YFQGLATITTQNISQGQCNFPIQIVASSASS 459

[Close]

BLAST of Carg26004 vs. TAIR 10
Match: AT5G58090.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 504.6 bits (1298), Expect = 8.9e-143
Identity = 248/446 (55.61%), Postives = 313/446 (70.18%), Query Frame = 0


Query: 17  ILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKLKLFEAEDRILEALIGT 76
           +LL    +     S+G NWGT A+H LPP+ VV+ML+ENG  K+KLF+AE   L AL  +
Sbjct: 8   LLLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKS 67

Query: 77  DIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTY 136
            IEVM+ +PN ML  ++   KAA  WV  NV+ +     VNI+YVAVGNEPFL +YNG+Y
Sbjct: 68  GIEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSY 127

Query: 137 LQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPEVRDLTIEI 196
           L  T PALRNIQ A+  AGL ++VK T P NAD+Y+S  S   PS GDFR  +RDL I I
Sbjct: 128 LSTTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITI 187

Query: 197 LHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDGDLTYTNVFDANFDTLV 256
           + FLS N  PFTVNIYP++SLY N  FP+D+AFFDG  +P+ DG   Y N+FDAN+DTLV
Sbjct: 188 VKFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTLV 247

Query: 257 SALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHALSGEGTPARVGVIDVY 316
            AL K G+ +M II+GEIGWPTDGD +AN+  AK+FNQG + H   G+GTP R G ID Y
Sbjct: 248 HALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAY 307

Query: 317 LFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIAVEGVRYMDKKWCV 376
           LFSLIDE+AKS++PG FERHWGIF FDG PKY L+L GT     LI  +GVRY+++KWCV
Sbjct: 308 LFSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCV 367

Query: 377 LNPNVK-DWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASYAFNMYFQMNSQKRWN 436
           + PNV+ D   +A  + YACSL DCT+L  G+SC  L  + N SYAFN Y+Q+  Q    
Sbjct: 368 MKPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTA 427

Query: 437 CEFSGLAMVTEQDPSYGNCQFPIMID 462
           C+F  ++ VT+ DPS G C+FPIMI+
Sbjct: 428 CKFPNISEVTKTDPSTGTCRFPIMIE 450

The following BLAST results are available for this feature:

BLAST of Carg26004 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021271.1	1.5e-285	100.00	Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022937442.1	7.5e-285	99.59	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita moschata]	[more]
XP_023537244.1	2.8e-284	99.38	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo]	[more]
XP_022965692.1	1.4e-278	97.54	glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima]	[more]
KAG6586415.1	1.4e-262	99.56	Glucan endo-1,3-beta-glucosidase 8, partial [Cucurbita argyrosperma subsp. soror...	[more]

BLAST of Carg26004 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NKW9	6.8e-156	59.65	Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ...	[more]
Q93Z08	1.3e-141	55.61	Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ...	[more]
Q9M088	2.9e-138	51.17	Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ...	[more]
Q9FGH4	1.3e-98	42.73	Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ...	[more]
Q9M069	1.3e-61	34.28	Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ...	[more]

BLAST of Carg26004 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FB83	3.6e-285	99.59	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 P...	[more]
A0A6J1HL09	6.6e-279	97.54	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=...	[more]
A0A6J1G3H9	1.0e-255	87.45	(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 P...	[more]
A0A6J1KB85	8.7e-255	87.40	(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111493909 PE=...	[more]
A0A6J1DUS6	3.3e-254	87.12	(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 ...	[more]

BLAST of Carg26004 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G24330.1	8.5e-186	65.73	O-Glycosyl hydrolases family 17 protein	[more]
AT2G19440.1	2.0e-158	59.74	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.1	4.9e-157	59.65	O-Glycosyl hydrolases family 17 protein	[more]
AT1G64760.2	4.9e-157	59.65	O-Glycosyl hydrolases family 17 protein	[more]
AT5G58090.1	8.9e-143	55.61	O-Glycosyl hydrolases family 17 protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012946	X8 domain	SMART	SM00768	X8_cls	coord: 373..456 e-value: 1.3E-37 score: 141.0
IPR012946	X8 domain	PFAM	PF07983	X8	coord: 373..443 e-value: 6.2E-18 score: 65.3
IPR000490	Glycoside hydrolase family 17	PFAM	PF00332	Glyco_hydro_17	coord: 31..351 e-value: 1.5E-66 score: 225.2
IPR000490	Glycoside hydrolase family 17	PROSITE	PS00587	GLYCOSYL_HYDROL_F17	coord: 267..280
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 29..359 e-value: 1.2E-109 score: 368.2
None	No IPR available	GENE3D	1.20.58.1040		coord: 368..464 e-value: 4.2E-8 score: 35.4
None	No IPR available	PANTHER	PTHR32227:SF319	BETA-1,3-GLUCANASE-LIKE PROTEIN	coord: 14..467
IPR044965	Glycoside hydrolase family 17, plant	PANTHER	PTHR32227	GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED	coord: 14..467
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 31..351

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg26004-RA	Carg26004-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0042973	glucan endo-1,3-beta-D-glucosidase activity
molecular_function	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds