Carg25963 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25963
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPentatricopeptide repeat-containing protein
LocationCarg_Chr20: 3896200 .. 3897630 (+)
RNA-Seq ExpressionCarg25963
SyntenyCarg25963
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGAGCCCTCACCATTTTCATATTAGACGGAGTCTCGATTCCTTCGCCAGTTTTTTGCGCCATGCTTCCCAAATCCAAAATCTCAAAGCCATTAAGAAGCTTCACGCCCAACTGCTTAGAACTCACTTCCAATTTTCCTCCCCGGATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCTAAGCTTAAGCAGCTTCTTCAAATGCATAATCCCCAGAAGTTCTTTAACCTTCAACGTAATGATTTCTGACTTTTATCGACATGGGTTTCCAGATTTTTCGTTAATGGCGTTGTCTTTCATGCATTCCAATGGGGTCCCTATCGATACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTGTTCAGAATCCCGTGTATGGAAAACAGATGCACGCCTTCGTGGGGAAATCAGGTTGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTCGATATGTATGCAAAAAATTCGCTGATTAATGATGCAGTGAAAGTGTTTGATGAAATTCCTGTGAAGAATACGGTGTGCGCAAATGCATTATTGGCTGGATATGGCGACGCTAGCATGTGGATTGATGGACTTGAATTGCTACGGAAGATGTACGTTTTGGATTTGAAGTATGACCAGTTCACTCTATCAGCTTCTTTACGTGCGTGCACTGGTTTATCTGCAATTGCTTTAGGTAAGCAAGTGCATGCTTATCTACTACGAACGGTTTATGATATTTCGAGTGATGTTCATCTACAAAGCACATTGATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTGTTTGTTTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGCATATGGTAGAAACGGATGTTACATAAGAGTAATTGAATTGTACAACCAGATGTTGATGGAAGGGATCAAACCAGATAGGGTGGCTTTTGTGACAGTGATATCAGCTTGTGGTCACACTGGTCAGGTGAATCTTGGAGTCAAGTATTTTGACTCCATGCAGAGTGTTTTTGGGTTGGAACCTGGTCAAGAGCACTATAGTTGTTTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTTGTAAGTGGTATGAAAAGTAGCGATTACAATGTACCTCTATGGGGTGCTTTACTCCGAGCTTGCTTGGAACATGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTACGAACGCTGGGATATTCGTTATGCTATCAAATTTGTATTCTGGTTTTGGCTTGTGGAATGAGATAGAGCATTTGAGGGGATTTGTGAGAAGGGAAGGGCTGCATAAAGGTGTTGGATGTAGCTGGATAGAGACCACAAGTTGA

mRNA sequence

ATGTCGAGCCCTCACCATTTTCATATTAGACGGAGTCTCGATTCCTTCGCCAGTTTTTTGCGCCATGCTTCCCAAATCCAAAATCTCAAAGCCATTAAGAAGCTTCACGCCCAACTGCTTAGAACTCACTTCCAATTTTCCTCCCCGGATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCTAAGCTTAAGCAGCTTCTTCAAATGCATAATCCCCAGAAGTTCTTTAACCTTCAACGTAATGATTTCTGACTTTTATCGACATGGGTTTCCAGATTTTTCGTTAATGGCGTTGTCTTTCATGCATTCCAATGGGGTCCCTATCGATACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTGTTCAGAATCCCGTGTATGGAAAACAGATGCACGCCTTCGTGGGGAAATCAGGTTGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTCGATATGTATGCAAAAAATTCGCTGATTAATGATGCAGTGAAAGTGTTTGATGAAATTCCTGTGAAGAATACGGTGTGCGCAAATGCATTATTGGCTGGATATGGCGACGCTAGCATGTGGATTGATGGACTTGAATTGCTACGGAAGATGTACGTTTTGGATTTGAAGTATGACCAGTTCACTCTATCAGCTTCTTTACGTGCGTGCACTGGTTTATCTGCAATTGCTTTAGGTAAGCAAGTGCATGCTTATCTACTACGAACGGTTTATGATATTTCGAGTGATGTTCATCTACAAAGCACATTGATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTGTTTGTTTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGCATATGGTAGAAACGGATGTTACATAAGAGTAATTGAATTGTACAACCAGATGTTGATGGAAGGGATCAAACCAGATAGGGTGGCTTTTGTGACAGTGATATCAGCTTGTGGTCACACTGGTCAGGTGAATCTTGGAGTCAAGTATTTTGACTCCATGCAGAGTGTTTTTGGGTTGGAACCTGGTCAAGAGCACTATAGTTGTTTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTTGTAAGTGGTATGAAAAGTAGCGATTACAATGTACCTCTATGGGGTGCTTTACTCCGAGCTTGCTTGGAACATGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTACGAACGCTGGGATATTCGTTATGCTATCAAATTTGTATTCTGGTTTTGGCTTGTGGAATGAGATAGAGCATTTGAGGGGATTTGTGAGAAGGGAAGGGCTGCATAAAGGTGTTGGATGTAGCTGGATAGAGACCACAAGTTGA

Coding sequence (CDS)

ATGTCGAGCCCTCACCATTTTCATATTAGACGGAGTCTCGATTCCTTCGCCAGTTTTTTGCGCCATGCTTCCCAAATCCAAAATCTCAAAGCCATTAAGAAGCTTCACGCCCAACTGCTTAGAACTCACTTCCAATTTTCCTCCCCGGATCTTCACTCTAAGCTTATACTATCCTACTCCCAAATCATCCCCAATTTTGATGCGCTAAGCTTAAGCAGCTTCTTCAAATGCATAATCCCCAGAAGTTCTTTAACCTTCAACGTAATGATTTCTGACTTTTATCGACATGGGTTTCCAGATTTTTCGTTAATGGCGTTGTCTTTCATGCATTCCAATGGGGTCCCTATCGATACGTATGCCCTCTGCAGCTCTTTGAGTGCTTGTTCTCTTGTTCAGAATCCCGTGTATGGAAAACAGATGCACGCCTTCGTGGGGAAATCAGGTTGGTTATGTAGTGTTTTTGTGGGGAGTGCGATTGTCGATATGTATGCAAAAAATTCGCTGATTAATGATGCAGTGAAAGTGTTTGATGAAATTCCTGTGAAGAATACGGTGTGCGCAAATGCATTATTGGCTGGATATGGCGACGCTAGCATGTGGATTGATGGACTTGAATTGCTACGGAAGATGTACGTTTTGGATTTGAAGTATGACCAGTTCACTCTATCAGCTTCTTTACGTGCGTGCACTGGTTTATCTGCAATTGCTTTAGGTAAGCAAGTGCATGCTTATCTACTACGAACGGTTTATGATATTTCGAGTGATGTTCATCTACAAAGCACATTGATTGAAATGTATGGGAAGAGTGGCATGGTGGAAAAAGCATGGAATGTGTTTGTTTTCGCAGGACTTGGACAAAAAGAAGATGGGAAAAGGGATATTATAATGTGGACTTCTATGCTTAGTGCATATGGTAGAAACGGATGTTACATAAGAGTAATTGAATTGTACAACCAGATGTTGATGGAAGGGATCAAACCAGATAGGGTGGCTTTTGTGACAGTGATATCAGCTTGTGGTCACACTGGTCAGGTGAATCTTGGAGTCAAGTATTTTGACTCCATGCAGAGTGTTTTTGGGTTGGAACCTGGTCAAGAGCACTATAGTTGTTTGGTTGACTTGCTCTGTAGAGCTGGGGAATTGGAAAAAGCATGGAAGCTTGTAAGTGGTATGAAAAGTAGCGATTACAATGTACCTCTATGGGGTGCTTTACTCCGAGCTTGCTTGGAACATGGAAATATTAAGTTGGGTAATTTGGCAGCTCGTAGGGCACTTGAATTGGATCCTACGAACGCTGGGATATTCGTTATGCTATCAAATTTGTATTCTGGTTTTGGCTTGTGGAATGAGATAGAGCATTTGAGGGGATTTGTGAGAAGGGAAGGGCTGCATAAAGGTGTTGGATGTAGCTGGATAGAGACCACAAGTTGA

Protein sequence

MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS
Homology
BLAST of Carg25963 vs. NCBI nr
Match: XP_022944055.1 (pentatricopeptide repeat-containing protein At1g50270-like [Cucurbita moschata] >KAG7010880.1 Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 967.2 bits (2499), Expect = 5.1e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 476

BLAST of Carg25963 vs. NCBI nr
Match: KAG6571067.1 (Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 964.1 bits (2491), Expect = 4.3e-277
Identity = 475/476 (99.79%), Postives = 475/476 (99.79%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLR FVRREGLHKGVGCSWIETTS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLREFVRREGLHKGVGCSWIETTS 476

BLAST of Carg25963 vs. NCBI nr
Match: XP_023512242.1 (pentatricopeptide repeat-containing protein At2g13600-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 928.7 bits (2399), Expect = 2.0e-266
Identity = 456/476 (95.80%), Postives = 468/476 (98.32%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MS+ HHFHIRRSLDSFASFLRH+SQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS
Sbjct: 14  MSNLHHFHIRRSLDSFASFLRHSSQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 73

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           QI+PNFDA SLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSL+ALSFMHSNGVPIDTYA
Sbjct: 74  QIVPNFDAQSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLIALSFMHSNGVPIDTYA 133

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCSSLSACSLVQN VYG+QMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP
Sbjct: 134 LCSSLSACSLVQNAVYGQQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 193

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           VKNTVCANALLAGYGDA MWIDGLELLRKM+VL+LKYDQFTLSASLRACTGLSAIALGKQ
Sbjct: 194 VKNTVCANALLAGYGDAGMWIDGLELLRKMHVLNLKYDQFTLSASLRACTGLSAIALGKQ 253

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLG KEDGKRDIIMWTSM
Sbjct: 254 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGHKEDGKRDIIMWTSM 313

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG
Sbjct: 314 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 373

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGEL+KAWKLVS +KSSD+NVPLWGALLRACLEHGNIKLGNLA
Sbjct: 374 LEPGQEHYSCLVDLLCRAGELDKAWKLVSDIKSSDHNVPLWGALLRACLEHGNIKLGNLA 433

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           AR ALELDPTNAGIFVMLSNLY+GFGLWNEIEHLR FVRREGL+KGVGCSWIETTS
Sbjct: 434 ARMALELDPTNAGIFVMLSNLYAGFGLWNEIEHLREFVRREGLYKGVGCSWIETTS 489

BLAST of Carg25963 vs. NCBI nr
Match: XP_038901137.1 (pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901138.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901139.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901140.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901141.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida] >XP_038901143.1 pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benincasa hispida])

HSP 1 Score: 847.8 bits (2189), Expect = 4.5e-242
Identity = 416/476 (87.39%), Postives = 441/476 (92.65%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MSSPHHF+IRRSLDSFASF RH+ Q +NLKAIK++HA LLRT FQF SP+LHSKLILSYS
Sbjct: 1   MSSPHHFYIRRSLDSFASFFRHSCQSKNLKAIKRIHAHLLRTRFQFFSPNLHSKLILSYS 60

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           QI PNFDA SL+S FKCIIPR SL FNV+ISDF RHGFPDF+LMA SFMHSNGVPIDTYA
Sbjct: 61  QINPNFDAQSLNSIFKCIIPRRSLNFNVIISDFSRHGFPDFALMAFSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCSSL++CSLVQN VYGKQ+HAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAV+VFDEIP
Sbjct: 121 LCSSLTSCSLVQNGVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVEVFDEIP 180

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLR+M  LDLKYDQFTLSASLRAC GLSAIALGKQ
Sbjct: 181 MKNTVCANALLAGYGEARMWVEGLELLREMQALDLKYDQFTLSASLRACAGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDI+ DVHLQSTLIEMYGKSGMVEKAWNVF FAGL QKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDITCDVHLQSTLIEMYGKSGMVEKAWNVFNFAGLRQKEDGKRDIIMWTSM 300

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LS YGRNGCY RVIELYNQMLMEG KPDRVAFVTVISACGHTG+VNLGVKYFDSMQS FG
Sbjct: 301 LSVYGRNGCYKRVIELYNQMLMEGNKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLV+ +KS DYNV LWGALLRACLE GNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVNDIKSWDYNVSLWGALLRACLEQGNIKLGNLA 420

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           A RALELDP NAGIFVMLSNLY+GFGLWNEIE LR FVR+EGL+K VGCSWIE TS
Sbjct: 421 AHRALELDPQNAGIFVMLSNLYAGFGLWNEIERLREFVRKEGLYKDVGCSWIEITS 476

BLAST of Carg25963 vs. NCBI nr
Match: XP_008457622.1 (PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >XP_008457623.1 PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >XP_016902170.1 PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis melo] >KAA0045664.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa] >TYJ99619.1 pentatricopeptide repeat-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 837.4 bits (2162), Expect = 6.1e-239
Identity = 406/466 (87.12%), Postives = 434/466 (93.13%), Query Frame = 0

Query: 11  RSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALS 70
           RSLDSFASF  H+SQI+NLK IKK+HA LLRTHFQF SP+LHSKLILSYSQIIPNFDA S
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSL 130
           L+S FKCIIPR+SLTFNVMISDF RHGFPDF+LM  SFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 VQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANAL 190
           VQN VYGKQ+HAFVGKSGWLCSVFVGSAIVDMYAKNSLI DAV+VFDE+P+KNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVY 250
           LAGYG+A MW++GLELLRKM  LDLKYDQFTLSASLRAC GLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCY 310
           DI+SDVHLQSTL+EMYGK+GMVEKAWNVF FAGLG +EDGKRDIIMWTSMLS YGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 IRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSC 370
            RVIELYNQMLMEGIKPDRVAFVTVISACGHTG+VNLGVKYFDSMQS FGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPT 430
           L DLLCRAGELEKAWKLV+ +KS DYNV LWGALLRACLE GNIKLGNLAARRALELDP 
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           NAGIF+MLSNLY+GFGLWNEIEHLR FVR+EGL+K VGCSWIE TS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Carg25963 vs. ExPASy Swiss-Prot
Match: Q9SN39 (Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DOT4 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.2e-65
Identity = 130/409 (31.78%), Postives = 229/409 (55.99%), Query Frame = 0

Query: 67  DALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLS 126
           D  S  + F+ +  RS +++  MI+ + R G    ++     M   G+  D Y + + L+
Sbjct: 346 DLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLN 405

Query: 127 ACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVC 186
            C+  +    GK++H ++ ++     +FV +A++DMYAK   + +A  VF E+ VK+ + 
Sbjct: 406 CCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIIS 465

Query: 187 ANALLAGYGDASMWIDGLELLRKMYVLDLKY--DQFTLSASLRACTGLSAIALGKQVHAY 246
            N ++ GY       + L L   + + + ++  D+ T++  L AC  LSA   G+++H Y
Sbjct: 466 WNTIIGGYSKNCYANEALSLF-NLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGY 525

Query: 247 LLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAY 306
           ++R  Y   SD H+ ++L++MY K G +  A  +F        +   +D++ WT M++ Y
Sbjct: 526 IMRNGY--FSDRHVANSLVDMYAKCGALLLAHMLF-------DDIASKDLVSWTVMIAGY 585

Query: 307 GRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPG 366
           G +G     I L+NQM   GI+ D ++FV+++ AC H+G V+ G ++F+ M+    +EP 
Sbjct: 586 GMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPT 645

Query: 367 QEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRA 426
            EHY+C+VD+L R G+L KA++ +  M     +  +WGALL  C  H ++KL    A + 
Sbjct: 646 VEHYACIVDMLARTGDLIKAYRFIENMPIPP-DATIWGALLCGCRIHHDVKLAEKVAEKV 705

Query: 427 LELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
            EL+P N G +V+++N+Y+    W +++ LR  + + GL K  GCSWIE
Sbjct: 706 FELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIE 743

BLAST of Carg25963 vs. ExPASy Swiss-Prot
Match: Q9SX45 (Pentatricopeptide repeat-containing protein At1g50270 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E42 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 4.2e-65
Identity = 154/454 (33.92%), Postives = 250/454 (55.07%), Query Frame = 0

Query: 29  LKAIKKL--------HAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSFFKCIIP 88
           LKA+ KL        HA +++     S P + + LI  YS     FD    S  F     
Sbjct: 111 LKAVFKLRDSNPFQFHAHIVKFGLD-SDPFVRNSLISGYSS-SGLFD--FASRLFDGAED 170

Query: 89  RSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNPVYGKQM 148
           +  +T+  MI  F R+G    +++    M   GV  +   + S L A   V++  +G+ +
Sbjct: 171 KDVVTWTAMIDGFVRNGSASEAMVYFVEMKKTGVAANEMTVVSVLKAAGKVEDVRFGRSV 230

Query: 149 HAFVGKSGWL-CSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGYGDASM 208
           H    ++G + C VF+GS++VDMY K S  +DA KVFDE+P +N V   AL+AGY  +  
Sbjct: 231 HGLYLETGRVKCDVFIGSSLVDMYGKCSCYDDAQKVFDEMPSRNVVTWTALIAGYVQSRC 290

Query: 209 WIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISSDVHLQ 268
           +  G+ +  +M   D+  ++ TLS+ L AC  + A+  G++VH Y+++   +I++     
Sbjct: 291 FDKGMLVFEEMLKSDVAPNEKTLSSVLSACAHVGALHRGRRVHCYMIKNSIEINTTA--G 350

Query: 269 STLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQ 328
           +TLI++Y K G +E+A  + VF  L +K     ++  WT+M++ +  +G      +L+  
Sbjct: 351 TTLIDLYVKCGCLEEA--ILVFERLHEK-----NVYTWTAMINGFAAHGYARDAFDLFYT 410

Query: 329 MLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAG 388
           ML   + P+ V F+ V+SAC H G V  G + F SM+  F +EP  +HY+C+VDL  R G
Sbjct: 411 MLSSHVSPNEVTFMAVLSACAHGGLVEEGRRLFLSMKGRFNMEPKADHYACMVDLFGRKG 470

Query: 389 ELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLS 448
            LE+A  L+  M     NV +WGAL  +CL H + +LG  AA R ++L P+++G + +L+
Sbjct: 471 LLEEAKALIERMPMEPTNV-VWGALFGSCLLHKDYELGKYAASRVIKLQPSHSGRYTLLA 530

Query: 449 NLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
           NLYS    W+E+  +R  ++ + + K  G SWIE
Sbjct: 531 NLYSESQNWDEVARVRKQMKDQQVVKSPGFSWIE 550

BLAST of Carg25963 vs. ExPASy Swiss-Prot
Match: P93005 (Pentatricopeptide repeat-containing protein At2g33680 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E19 PE=3 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.6e-64
Identity = 135/393 (34.35%), Postives = 219/393 (55.73%), Query Frame = 0

Query: 81  RSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNPVYGKQM 140
           R+S+T++ M++ + ++G    ++   S M S G+    Y +   L+ACS +     GKQ+
Sbjct: 285 RNSITWSAMVTGYSQNGESLEAVKLFSRMFSAGIKPSEYTIVGVLNACSDICYLEEGKQL 344

Query: 141 HAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGYGDASMW 200
           H+F+ K G+   +F  +A+VDMYAK   + DA K FD +  ++     +L++GY   S  
Sbjct: 345 HSFLLKLGFERHLFATTALVDMYAKAGCLADARKGFDCLQERDVALWTSLISGYVQNSDN 404

Query: 201 IDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISSDVHLQS 260
            + L L R+M    +  +  T+++ L+AC+ L+ + LGKQVH + ++  +    +V + S
Sbjct: 405 EEALILYRRMKTAGIIPNDPTMASVLKACSSLATLELGKQVHGHTIK--HGFGLEVPIGS 464

Query: 261 TLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQM 320
            L  MY K G +E    VF       +    +D++ W +M+S    NG     +EL+ +M
Sbjct: 465 ALSTMYSKCGSLEDGNLVF-------RRTPNKDVVSWNAMISGLSHNGQGDEALELFEEM 524

Query: 321 LMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAGE 380
           L EG++PD V FV +ISAC H G V  G  YF+ M    GL+P  +HY+C+VDLL RAG+
Sbjct: 525 LAEGMEPDDVTFVNIISACSHKGFVERGWFYFNMMSDQIGLDPKVDHYACMVDLLSRAGQ 584

Query: 381 LEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLSN 440
           L++A + +    + D+ + LW  LL AC  HG  +LG  A  + + L    +  +V LS 
Sbjct: 585 LKEAKEFIES-ANIDHGLCLWRILLSACKNHGKCELGVYAGEKLMALGSRESSTYVQLSG 644

Query: 441 LYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
           +Y+  G   ++E +   +R  G+ K VGCSWIE
Sbjct: 645 IYTALGRMRDVERVWKHMRANGVSKEVGCSWIE 667

BLAST of Carg25963 vs. ExPASy Swiss-Prot
Match: Q9SIT7 (Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E76 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.5e-64
Identity = 157/495 (31.72%), Postives = 262/495 (52.93%), Query Frame = 0

Query: 15  SFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSF 74
           SFAS L   S + ++    ++H+ + ++ F  S   + S L+  YS+     DA      
Sbjct: 154 SFASVLSACSGLNDMNKGVQVHSLIAKSPF-LSDVYIGSALVDMYSKCGNVNDA---QRV 213

Query: 75  FKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNP 134
           F  +  R+ +++N +I+ F ++G    +L     M  + V  D   L S +SAC+ +   
Sbjct: 214 FDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAI 273

Query: 135 VYGKQMHAFVGKSGWLCS-VFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAG 194
             G+++H  V K+  L + + + +A VDMYAK S I +A  +FD +P++N +   ++++G
Sbjct: 274 KVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISG 333

Query: 195 YGDASMWIDGLELLRKM--------YVLDLKYDQ-----------------------FTL 254
           Y  A+       +  KM          L   Y Q                       ++ 
Sbjct: 334 YAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSF 393

Query: 255 SASLRACTGLSAIALGKQVHAYLLRTVYDISS----DVHLQSTLIEMYGKSGMVEKAWNV 314
           +  L+AC  L+ + LG Q H ++L+  +   S    D+ + ++LI+MY K G VE+ + V
Sbjct: 394 ANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLV 453

Query: 315 FVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISA 374
           F       ++  +RD + W +M+  + +NG     +EL+ +ML  G KPD +  + V+SA
Sbjct: 454 F-------RKMMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSA 513

Query: 375 CGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNV 434
           CGH G V  G  YF SM   FG+ P ++HY+C+VDLL RAG LE+A  ++  M     +V
Sbjct: 514 CGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSV 573

Query: 435 PLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFV 474
            +WG+LL AC  H NI LG   A + LE++P+N+G +V+LSN+Y+  G W ++ ++R  +
Sbjct: 574 -IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSM 633

BLAST of Carg25963 vs. ExPASy Swiss-Prot
Match: Q9STE1 (Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana OX=3702 GN=PCMP-E36 PE=3 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 7.9e-64
Identity = 141/460 (30.65%), Postives = 259/460 (56.30%), Query Frame = 0

Query: 15  SFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSF 74
           +F+S L   S+ +NL+  K++H  ++R H       L S LI +Y +      +++ + F
Sbjct: 342 TFSSLLPSVSKFENLEYCKQIHCYIMR-HSISLDIFLTSALIDAYFKC--RGVSMAQNIF 401

Query: 75  FKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNP 134
            +C      + F  MIS +  +G    SL    ++    +  +   L S L    ++   
Sbjct: 402 SQC-NSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLAL 461

Query: 135 VYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGY 194
             G+++H F+ K G+     +G A++DMYAK   +N A ++F+ +  ++ V  N+++   
Sbjct: 462 KLGRELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRC 521

Query: 195 GDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISS 254
             +      +++ R+M V  + YD  ++SA+L AC  L + + GK +H ++++  + ++S
Sbjct: 522 AQSDNPSAAIDIFRQMGVSGICYDCVSISAALSACANLPSESFGKAIHGFMIK--HSLAS 581

Query: 255 DVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVI 314
           DV+ +STLI+MY K G ++ A NVF       K   +++I+ W S+++A G +G     +
Sbjct: 582 DVYSESTLIDMYAKCGNLKAAMNVF-------KTMKEKNIVSWNSIIAACGNHGKLKDSL 641

Query: 315 ELYNQML-MEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVD 374
            L+++M+   GI+PD++ F+ +IS+C H G V+ GV++F SM   +G++P QEHY+C+VD
Sbjct: 642 CLFHEMVEKSGIRPDQITFLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHYACVVD 701

Query: 375 LLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAG 434
           L  RAG L +A++ V  M     +  +WG LL AC  H N++L  +A+ + ++LDP+N+G
Sbjct: 702 LFGRAGRLTEAYETVKSMPFPP-DAGVWGTLLGACRLHKNVELAEVASSKLMDLDPSNSG 761

Query: 435 IFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
            +V++SN ++    W  +  +R  ++   + K  G SWIE
Sbjct: 762 YYVLISNAHANAREWESVTKVRSLMKEREVQKIPGYSWIE 787

BLAST of Carg25963 vs. ExPASy TrEMBL
Match: A0A6J1FYF9 (pentatricopeptide repeat-containing protein At1g50270-like OS=Cucurbita moschata OX=3662 GN=LOC111448605 PE=4 SV=1)

HSP 1 Score: 967.2 bits (2499), Expect = 2.5e-278
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS
Sbjct: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA
Sbjct: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP
Sbjct: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ
Sbjct: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM
Sbjct: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG
Sbjct: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA
Sbjct: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS
Sbjct: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 476

BLAST of Carg25963 vs. ExPASy TrEMBL
Match: A0A1S3C6K8 (pentatricopeptide repeat-containing protein At2g13600-like OS=Cucumis melo OX=3656 GN=LOC103497272 PE=4 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.9e-239
Identity = 406/466 (87.12%), Postives = 434/466 (93.13%), Query Frame = 0

Query: 11  RSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALS 70
           RSLDSFASF  H+SQI+NLK IKK+HA LLRTHFQF SP+LHSKLILSYSQIIPNFDA S
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSL 130
           L+S FKCIIPR+SLTFNVMISDF RHGFPDF+LM  SFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 VQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANAL 190
           VQN VYGKQ+HAFVGKSGWLCSVFVGSAIVDMYAKNSLI DAV+VFDE+P+KNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVY 250
           LAGYG+A MW++GLELLRKM  LDLKYDQFTLSASLRAC GLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCY 310
           DI+SDVHLQSTL+EMYGK+GMVEKAWNVF FAGLG +EDGKRDIIMWTSMLS YGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 IRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSC 370
            RVIELYNQMLMEGIKPDRVAFVTVISACGHTG+VNLGVKYFDSMQS FGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPT 430
           L DLLCRAGELEKAWKLV+ +KS DYNV LWGALLRACLE GNIKLGNLAARRALELDP 
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           NAGIF+MLSNLY+GFGLWNEIEHLR FVR+EGL+K VGCSWIE TS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Carg25963 vs. ExPASy TrEMBL
Match: A0A5D3BKX2 (Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001930 PE=4 SV=1)

HSP 1 Score: 837.4 bits (2162), Expect = 2.9e-239
Identity = 406/466 (87.12%), Postives = 434/466 (93.13%), Query Frame = 0

Query: 11  RSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALS 70
           RSLDSFASF  H+SQI+NLK IKK+HA LLRTHFQF SP+LHSKLILSYSQIIPNFDA S
Sbjct: 4   RSLDSFASFFGHSSQIKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAQS 63

Query: 71  LSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSL 130
           L+S FKCIIPR+SLTFNVMISDF RHGFPDF+LM  SFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNSIFKCIIPRTSLTFNVMISDFSRHGFPDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 VQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANAL 190
           VQN VYGKQ+HAFVGKSGWLCSVFVGSAIVDMYAKNSLI DAV+VFDE+P+KNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVY 250
           LAGYG+A MW++GLELLRKM  LDLKYDQFTLSASLRAC GLSAIALGKQ+HAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACAGLSAIALGKQLHAYILRTVY 243

Query: 251 DISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCY 310
           DI+SDVHLQSTL+EMYGK+GMVEKAWNVF FAGLG +EDGKRDIIMWTSMLS YGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFNFAGLGHREDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 IRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSC 370
            RVIELYNQMLMEGIKPDRVAFVTVISACGHTG+VNLGVKYFDSMQS FGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVKYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPT 430
           L DLLCRAGELEKAWKLV+ +KS DYNV LWGALLRACLE GNIKLGNLAARRALELDP 
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           NAGIF+MLSNLY+GFGLWNEIEHLR FVR+EGL+K VGCSWIE TS
Sbjct: 424 NAGIFIMLSNLYAGFGLWNEIEHLREFVRKEGLYKDVGCSWIEITS 469

BLAST of Carg25963 vs. ExPASy TrEMBL
Match: A0A0A0LLD6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G258600 PE=4 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 4.4e-235
Identity = 400/466 (85.84%), Postives = 429/466 (92.06%), Query Frame = 0

Query: 11  RSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALS 70
           RSLDSFASF  H+SQ +NLK IKK+HA LLRTHFQF SP+LHSKLILSYSQIIPNFDA S
Sbjct: 4   RSLDSFASFFHHSSQFKNLKTIKKIHAHLLRTHFQFFSPNLHSKLILSYSQIIPNFDAKS 63

Query: 71  LSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSL 130
           L+  F+CIIPR+SLTFNVMISDF RHGF DF+LM  SFMHSNGVPIDTYALCSSL+ CS 
Sbjct: 64  LNGIFQCIIPRTSLTFNVMISDFSRHGFQDFALMGFSFMHSNGVPIDTYALCSSLNGCSF 123

Query: 131 VQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANAL 190
           VQN VYGKQ+HAFVGKSGWLCSVFVGSAIVDMYAKNSLI DAV+VFDE+P+KNTVCANAL
Sbjct: 124 VQNVVYGKQIHAFVGKSGWLCSVFVGSAIVDMYAKNSLIYDAVEVFDEMPMKNTVCANAL 183

Query: 191 LAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVY 250
           LAGYG+A MW++GLELLRKM  LDLKYDQFTLSASLRACTGLSAIALGKQVHAY+LRTVY
Sbjct: 184 LAGYGEARMWVEGLELLRKMQGLDLKYDQFTLSASLRACTGLSAIALGKQVHAYILRTVY 243

Query: 251 DISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCY 310
           DI+SDVHLQSTL+EMYGK+GMVEKAWNVF FAGLG++EDGKRDIIMWTSMLS YGRNGCY
Sbjct: 244 DITSDVHLQSTLVEMYGKNGMVEKAWNVFKFAGLGRQEDGKRDIIMWTSMLSVYGRNGCY 303

Query: 311 IRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSC 370
            RVIELYNQMLMEGIKPDRVAFVTVISACGHTG+VNLGV+YFDSMQS FGLEPGQEHYSC
Sbjct: 304 KRVIELYNQMLMEGIKPDRVAFVTVISACGHTGEVNLGVQYFDSMQSDFGLEPGQEHYSC 363

Query: 371 LVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPT 430
           L DLLCRAGELEKAWKLV+ +KS DYNV LWGALLRACLE GNIKLGNLAARRALELDP 
Sbjct: 364 LADLLCRAGELEKAWKLVNDIKSRDYNVSLWGALLRACLEQGNIKLGNLAARRALELDPQ 423

Query: 431 NAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           N GI VMLSNLY+GFGLWNEIEHLR  VR+EGL+K V CSWIE TS
Sbjct: 424 NTGILVMLSNLYAGFGLWNEIEHLRESVRKEGLYKDVACSWIEITS 469

BLAST of Carg25963 vs. ExPASy TrEMBL
Match: A0A6J1CYG8 (pentatricopeptide repeat-containing protein At4g21300-like OS=Momordica charantia OX=3673 GN=LOC111015438 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 1.9e-225
Identity = 386/476 (81.09%), Postives = 427/476 (89.71%), Query Frame = 0

Query: 1   MSSPHHFHIRRSLDSFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYS 60
           MS  HHFHI++SLD F+SFLRH+++ +NL  IKKLHA L+RTHFQF SP LH+KLILSYS
Sbjct: 1   MSRSHHFHIKQSLDFFSSFLRHSTETKNLTPIKKLHAHLIRTHFQFFSPSLHTKLILSYS 60

Query: 61  QIIPNFDALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYA 120
           +  PNF A SLSS FK IIPRSSLTFN++I+DF R+G  DF+LMA SFMHSNGVPID+YA
Sbjct: 61  RANPNFGAQSLSSLFKFIIPRSSLTFNIIIADFCRNGSADFALMAFSFMHSNGVPIDSYA 120

Query: 121 LCSSLSACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIP 180
           LCS+LSACSLVQN ++GKQMHAFVGKSGWL SVFVGSAIVD+YAKN LI DAV+VFDEIP
Sbjct: 121 LCSTLSACSLVQNVMFGKQMHAFVGKSGWLSSVFVGSAIVDLYAKNGLIGDAVEVFDEIP 180

Query: 181 VKNTVCANALLAGYGDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQ 240
           +KNTVCANALLAGYG+A MW++GLELLRKMYVLDLKYD FTLSASLR C  LSAIALGKQ
Sbjct: 181 MKNTVCANALLAGYGEAKMWVEGLELLRKMYVLDLKYDHFTLSASLRTCACLSAIALGKQ 240

Query: 241 VHAYLLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSM 300
           VHAYLLR +YDI SDVHLQSTLIEMYGKSGMVEKAWNVF FAGL QKEDGKRDII+WTS+
Sbjct: 241 VHAYLLRMIYDIPSDVHLQSTLIEMYGKSGMVEKAWNVFNFAGLEQKEDGKRDIIVWTSI 300

Query: 301 LSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFG 360
           LS YGRNGCY RVIELY+QMLMEGIKPDRVAFVTVISACGHTG+V+LG+KYFDSMQS FG
Sbjct: 301 LSVYGRNGCYKRVIELYSQMLMEGIKPDRVAFVTVISACGHTGEVDLGIKYFDSMQSDFG 360

Query: 361 LEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLA 420
           L+PGQEHYSCLVDLLCRAGEL+KAWKLV+ MKS   NV LWGALLRACLEHG I+LGNLA
Sbjct: 361 LQPGQEHYSCLVDLLCRAGELDKAWKLVNDMKSQHCNVSLWGALLRACLEHGKIELGNLA 420

Query: 421 ARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIETTS 477
           A++ALELDP N GIFVMLSNLY+ FGLWNEIEHLR FV +EG+ K VGCSWIE TS
Sbjct: 421 AQKALELDPQNTGIFVMLSNLYAIFGLWNEIEHLREFVSKEGMCKDVGCSWIEITS 476

BLAST of Carg25963 vs. TAIR 10
Match: AT1G50270.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 154/454 (33.92%), Postives = 250/454 (55.07%), Query Frame = 0

Query: 29  LKAIKKL--------HAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSFFKCIIP 88
           LKA+ KL        HA +++     S P + + LI  YS     FD    S  F     
Sbjct: 111 LKAVFKLRDSNPFQFHAHIVKFGLD-SDPFVRNSLISGYSS-SGLFD--FASRLFDGAED 170

Query: 89  RSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNPVYGKQM 148
           +  +T+  MI  F R+G    +++    M   GV  +   + S L A   V++  +G+ +
Sbjct: 171 KDVVTWTAMIDGFVRNGSASEAMVYFVEMKKTGVAANEMTVVSVLKAAGKVEDVRFGRSV 230

Query: 149 HAFVGKSGWL-CSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGYGDASM 208
           H    ++G + C VF+GS++VDMY K S  +DA KVFDE+P +N V   AL+AGY  +  
Sbjct: 231 HGLYLETGRVKCDVFIGSSLVDMYGKCSCYDDAQKVFDEMPSRNVVTWTALIAGYVQSRC 290

Query: 209 WIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISSDVHLQ 268
           +  G+ +  +M   D+  ++ TLS+ L AC  + A+  G++VH Y+++   +I++     
Sbjct: 291 FDKGMLVFEEMLKSDVAPNEKTLSSVLSACAHVGALHRGRRVHCYMIKNSIEINTTA--G 350

Query: 269 STLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQ 328
           +TLI++Y K G +E+A  + VF  L +K     ++  WT+M++ +  +G      +L+  
Sbjct: 351 TTLIDLYVKCGCLEEA--ILVFERLHEK-----NVYTWTAMINGFAAHGYARDAFDLFYT 410

Query: 329 MLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAG 388
           ML   + P+ V F+ V+SAC H G V  G + F SM+  F +EP  +HY+C+VDL  R G
Sbjct: 411 MLSSHVSPNEVTFMAVLSACAHGGLVEEGRRLFLSMKGRFNMEPKADHYACMVDLFGRKG 470

Query: 389 ELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLS 448
            LE+A  L+  M     NV +WGAL  +CL H + +LG  AA R ++L P+++G + +L+
Sbjct: 471 LLEEAKALIERMPMEPTNV-VWGALFGSCLLHKDYELGKYAASRVIKLQPSHSGRYTLLA 530

Query: 449 NLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
           NLYS    W+E+  +R  ++ + + K  G SWIE
Sbjct: 531 NLYSESQNWDEVARVRKQMKDQQVVKSPGFSWIE 550

BLAST of Carg25963 vs. TAIR 10
Match: AT4G18750.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-66
Identity = 130/409 (31.78%), Postives = 229/409 (55.99%), Query Frame = 0

Query: 67  DALSLSSFFKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLS 126
           D  S  + F+ +  RS +++  MI+ + R G    ++     M   G+  D Y + + L+
Sbjct: 346 DLDSAKAVFREMSDRSVVSYTSMIAGYAREGLAGEAVKLFEEMEEEGISPDVYTVTAVLN 405

Query: 127 ACSLVQNPVYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVC 186
            C+  +    GK++H ++ ++     +FV +A++DMYAK   + +A  VF E+ VK+ + 
Sbjct: 406 CCARYRLLDEGKRVHEWIKENDLGFDIFVSNALMDMYAKCGSMQEAELVFSEMRVKDIIS 465

Query: 187 ANALLAGYGDASMWIDGLELLRKMYVLDLKY--DQFTLSASLRACTGLSAIALGKQVHAY 246
            N ++ GY       + L L   + + + ++  D+ T++  L AC  LSA   G+++H Y
Sbjct: 466 WNTIIGGYSKNCYANEALSLF-NLLLEEKRFSPDERTVACVLPACASLSAFDKGREIHGY 525

Query: 247 LLRTVYDISSDVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAY 306
           ++R  Y   SD H+ ++L++MY K G +  A  +F        +   +D++ WT M++ Y
Sbjct: 526 IMRNGY--FSDRHVANSLVDMYAKCGALLLAHMLF-------DDIASKDLVSWTVMIAGY 585

Query: 307 GRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPG 366
           G +G     I L+NQM   GI+ D ++FV+++ AC H+G V+ G ++F+ M+    +EP 
Sbjct: 586 GMHGFGKEAIALFNQMRQAGIEADEISFVSLLYACSHSGLVDEGWRFFNIMRHECKIEPT 645

Query: 367 QEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRA 426
            EHY+C+VD+L R G+L KA++ +  M     +  +WGALL  C  H ++KL    A + 
Sbjct: 646 VEHYACIVDMLARTGDLIKAYRFIENMPIPP-DATIWGALLCGCRIHHDVKLAEKVAEKV 705

Query: 427 LELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
            EL+P N G +V+++N+Y+    W +++ LR  + + GL K  GCSWIE
Sbjct: 706 FELEPENTGYYVLMANIYAEAEKWEQVKRLRKRIGQRGLRKNPGCSWIE 743

BLAST of Carg25963 vs. TAIR 10
Match: AT2G33680.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-65
Identity = 135/393 (34.35%), Postives = 219/393 (55.73%), Query Frame = 0

Query: 81  RSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNPVYGKQM 140
           R+S+T++ M++ + ++G    ++   S M S G+    Y +   L+ACS +     GKQ+
Sbjct: 285 RNSITWSAMVTGYSQNGESLEAVKLFSRMFSAGIKPSEYTIVGVLNACSDICYLEEGKQL 344

Query: 141 HAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGYGDASMW 200
           H+F+ K G+   +F  +A+VDMYAK   + DA K FD +  ++     +L++GY   S  
Sbjct: 345 HSFLLKLGFERHLFATTALVDMYAKAGCLADARKGFDCLQERDVALWTSLISGYVQNSDN 404

Query: 201 IDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISSDVHLQS 260
            + L L R+M    +  +  T+++ L+AC+ L+ + LGKQVH + ++  +    +V + S
Sbjct: 405 EEALILYRRMKTAGIIPNDPTMASVLKACSSLATLELGKQVHGHTIK--HGFGLEVPIGS 464

Query: 261 TLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQM 320
            L  MY K G +E    VF       +    +D++ W +M+S    NG     +EL+ +M
Sbjct: 465 ALSTMYSKCGSLEDGNLVF-------RRTPNKDVVSWNAMISGLSHNGQGDEALELFEEM 524

Query: 321 LMEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAGE 380
           L EG++PD V FV +ISAC H G V  G  YF+ M    GL+P  +HY+C+VDLL RAG+
Sbjct: 525 LAEGMEPDDVTFVNIISACSHKGFVERGWFYFNMMSDQIGLDPKVDHYACMVDLLSRAGQ 584

Query: 381 LEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLSN 440
           L++A + +    + D+ + LW  LL AC  HG  +LG  A  + + L    +  +V LS 
Sbjct: 585 LKEAKEFIES-ANIDHGLCLWRILLSACKNHGKCELGVYAGEKLMALGSRESSTYVQLSG 644

Query: 441 LYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
           +Y+  G   ++E +   +R  G+ K VGCSWIE
Sbjct: 645 IYTALGRMRDVERVWKHMRANGVSKEVGCSWIE 667

BLAST of Carg25963 vs. TAIR 10
Match: AT2G13600.1 (Pentatricopeptide repeat (PPR) superfamily protein )

HSP 1 Score: 247.3 bits (630), Expect = 2.5e-65
Identity = 157/495 (31.72%), Postives = 262/495 (52.93%), Query Frame = 0

Query: 15  SFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSF 74
           SFAS L   S + ++    ++H+ + ++ F  S   + S L+  YS+     DA      
Sbjct: 154 SFASVLSACSGLNDMNKGVQVHSLIAKSPF-LSDVYIGSALVDMYSKCGNVNDA---QRV 213

Query: 75  FKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNP 134
           F  +  R+ +++N +I+ F ++G    +L     M  + V  D   L S +SAC+ +   
Sbjct: 214 FDEMGDRNVVSWNSLITCFEQNGPAVEALDVFQMMLESRVEPDEVTLASVISACASLSAI 273

Query: 135 VYGKQMHAFVGKSGWLCS-VFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAG 194
             G+++H  V K+  L + + + +A VDMYAK S I +A  +FD +P++N +   ++++G
Sbjct: 274 KVGQEVHGRVVKNDKLRNDIILSNAFVDMYAKCSRIKEARFIFDSMPIRNVIAETSMISG 333

Query: 195 YGDASMWIDGLELLRKM--------YVLDLKYDQ-----------------------FTL 254
           Y  A+       +  KM          L   Y Q                       ++ 
Sbjct: 334 YAMAASTKAARLMFTKMAERNVVSWNALIAGYTQNGENEEALSLFCLLKRESVCPTHYSF 393

Query: 255 SASLRACTGLSAIALGKQVHAYLLRTVYDISS----DVHLQSTLIEMYGKSGMVEKAWNV 314
           +  L+AC  L+ + LG Q H ++L+  +   S    D+ + ++LI+MY K G VE+ + V
Sbjct: 394 ANILKACADLAELHLGMQAHVHVLKHGFKFQSGEEDDIFVGNSLIDMYVKCGCVEEGYLV 453

Query: 315 FVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVIELYNQMLMEGIKPDRVAFVTVISA 374
           F       ++  +RD + W +M+  + +NG     +EL+ +ML  G KPD +  + V+SA
Sbjct: 454 F-------RKMMERDCVSWNAMIIGFAQNGYGNEALELFREMLESGEKPDHITMIGVLSA 513

Query: 375 CGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVDLLCRAGELEKAWKLVSGMKSSDYNV 434
           CGH G V  G  YF SM   FG+ P ++HY+C+VDLL RAG LE+A  ++  M     +V
Sbjct: 514 CGHAGFVEEGRHYFSSMTRDFGVAPLRDHYTCMVDLLGRAGFLEEAKSMIEEMPMQPDSV 573

Query: 435 PLWGALLRACLEHGNIKLGNLAARRALELDPTNAGIFVMLSNLYSGFGLWNEIEHLRGFV 474
            +WG+LL AC  H NI LG   A + LE++P+N+G +V+LSN+Y+  G W ++ ++R  +
Sbjct: 574 -IWGSLLAACKVHRNITLGKYVAEKLLEVEPSNSGPYVLLSNMYAELGKWEDVMNVRKSM 633

BLAST of Carg25963 vs. TAIR 10
Match: AT4G21300.1 (Tetratricopeptide repeat (TPR)-like superfamily protein )

HSP 1 Score: 246.1 bits (627), Expect = 5.6e-65
Identity = 141/460 (30.65%), Postives = 259/460 (56.30%), Query Frame = 0

Query: 15  SFASFLRHASQIQNLKAIKKLHAQLLRTHFQFSSPDLHSKLILSYSQIIPNFDALSLSSF 74
           +F+S L   S+ +NL+  K++H  ++R H       L S LI +Y +      +++ + F
Sbjct: 342 TFSSLLPSVSKFENLEYCKQIHCYIMR-HSISLDIFLTSALIDAYFKC--RGVSMAQNIF 401

Query: 75  FKCIIPRSSLTFNVMISDFYRHGFPDFSLMALSFMHSNGVPIDTYALCSSLSACSLVQNP 134
            +C      + F  MIS +  +G    SL    ++    +  +   L S L    ++   
Sbjct: 402 SQC-NSVDVVVFTAMISGYLHNGLYIDSLEMFRWLVKVKISPNEITLVSILPVIGILLAL 461

Query: 135 VYGKQMHAFVGKSGWLCSVFVGSAIVDMYAKNSLINDAVKVFDEIPVKNTVCANALLAGY 194
             G+++H F+ K G+     +G A++DMYAK   +N A ++F+ +  ++ V  N+++   
Sbjct: 462 KLGRELHGFIIKKGFDNRCNIGCAVIDMYAKCGRMNLAYEIFERLSKRDIVSWNSMITRC 521

Query: 195 GDASMWIDGLELLRKMYVLDLKYDQFTLSASLRACTGLSAIALGKQVHAYLLRTVYDISS 254
             +      +++ R+M V  + YD  ++SA+L AC  L + + GK +H ++++  + ++S
Sbjct: 522 AQSDNPSAAIDIFRQMGVSGICYDCVSISAALSACANLPSESFGKAIHGFMIK--HSLAS 581

Query: 255 DVHLQSTLIEMYGKSGMVEKAWNVFVFAGLGQKEDGKRDIIMWTSMLSAYGRNGCYIRVI 314
           DV+ +STLI+MY K G ++ A NVF       K   +++I+ W S+++A G +G     +
Sbjct: 582 DVYSESTLIDMYAKCGNLKAAMNVF-------KTMKEKNIVSWNSIIAACGNHGKLKDSL 641

Query: 315 ELYNQML-MEGIKPDRVAFVTVISACGHTGQVNLGVKYFDSMQSVFGLEPGQEHYSCLVD 374
            L+++M+   GI+PD++ F+ +IS+C H G V+ GV++F SM   +G++P QEHY+C+VD
Sbjct: 642 CLFHEMVEKSGIRPDQITFLEIISSCCHVGDVDEGVRFFRSMTEDYGIQPQQEHYACVVD 701

Query: 375 LLCRAGELEKAWKLVSGMKSSDYNVPLWGALLRACLEHGNIKLGNLAARRALELDPTNAG 434
           L  RAG L +A++ V  M     +  +WG LL AC  H N++L  +A+ + ++LDP+N+G
Sbjct: 702 LFGRAGRLTEAYETVKSMPFPP-DAGVWGTLLGACRLHKNVELAEVASSKLMDLDPSNSG 761

Query: 435 IFVMLSNLYSGFGLWNEIEHLRGFVRREGLHKGVGCSWIE 474
            +V++SN ++    W  +  +R  ++   + K  G SWIE
Sbjct: 762 YYVLISNAHANAREWESVTKVRSLMKEREVQKIPGYSWIE 787

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944055.15.1e-278100.00pentatricopeptide repeat-containing protein At1g50270-like [Cucurbita moschata] ... [more]
KAG6571067.14.3e-27799.79Pentatricopeptide repeat-containing protein, partial [Cucurbita argyrosperma sub... [more]
XP_023512242.12.0e-26695.80pentatricopeptide repeat-containing protein At2g13600-like [Cucurbita pepo subsp... [more]
XP_038901137.14.5e-24287.39pentatricopeptide repeat-containing protein At5g04780, mitochondrial-like [Benin... [more]
XP_008457622.16.1e-23987.12PREDICTED: pentatricopeptide repeat-containing protein At2g13600-like [Cucumis m... [more]
Match NameE-valueIdentityDescription
Q9SN394.2e-6531.78Pentatricopeptide repeat-containing protein DOT4, chloroplastic OS=Arabidopsis t... [more]
Q9SX454.2e-6533.92Pentatricopeptide repeat-containing protein At1g50270 OS=Arabidopsis thaliana OX... [more]
P930051.6e-6434.35Pentatricopeptide repeat-containing protein At2g33680 OS=Arabidopsis thaliana OX... [more]
Q9SIT73.5e-6431.72Pentatricopeptide repeat-containing protein At2g13600 OS=Arabidopsis thaliana OX... [more]
Q9STE17.9e-6430.65Pentatricopeptide repeat-containing protein At4g21300 OS=Arabidopsis thaliana OX... [more]
Match NameE-valueIdentityDescription
A0A6J1FYF92.5e-278100.00pentatricopeptide repeat-containing protein At1g50270-like OS=Cucurbita moschata... [more]
A0A1S3C6K82.9e-23987.12pentatricopeptide repeat-containing protein At2g13600-like OS=Cucumis melo OX=36... [more]
A0A5D3BKX22.9e-23987.12Pentatricopeptide repeat-containing protein OS=Cucumis melo var. makuwa OX=11946... [more]
A0A0A0LLD64.4e-23585.84Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G258600 PE=4 SV=1[more]
A0A6J1CYG81.9e-22581.09pentatricopeptide repeat-containing protein At4g21300-like OS=Momordica charanti... [more]
Match NameE-valueIdentityDescription
AT1G50270.13.0e-6633.92Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G18750.13.0e-6631.78Pentatricopeptide repeat (PPR) superfamily protein [more]
AT2G33680.11.1e-6534.35Tetratricopeptide repeat (TPR)-like superfamily protein [more]
AT2G13600.12.5e-6531.72Pentatricopeptide repeat (PPR) superfamily protein [more]
AT4G21300.15.6e-6530.65Tetratricopeptide repeat (TPR)-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002885Pentatricopeptide repeatPFAMPF13041PPR_2coord: 293..339
e-value: 1.8E-7
score: 31.2
IPR002885Pentatricopeptide repeatTIGRFAMTIGR00756TIGR00756coord: 368..395
e-value: 1.0E-5
score: 23.4
coord: 295..328
e-value: 7.2E-8
score: 30.1
coord: 331..363
e-value: 7.5E-4
score: 17.5
IPR002885Pentatricopeptide repeatPFAMPF01535PPRcoord: 158..180
e-value: 0.28
score: 11.5
coord: 186..210
e-value: 0.43
score: 10.9
coord: 260..279
e-value: 0.055
score: 13.7
coord: 85..114
e-value: 0.24
score: 11.7
IPR002885Pentatricopeptide repeatPFAMPF12854PPR_1coord: 360..392
e-value: 9.4E-7
score: 28.5
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 293..327
score: 11.520384
IPR002885Pentatricopeptide repeatPROSITEPS51375PPRcoord: 364..398
score: 9.317163
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 131..235
e-value: 3.9E-13
score: 51.1
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 250..473
e-value: 3.4E-37
score: 130.5
NoneNo IPR availablePANTHERPTHR47928REPEAT-CONTAINING PROTEIN, PUTATIVE-RELATEDcoord: 29..472
NoneNo IPR availablePANTHERPTHR47928:SF115OS02G0126500 PROTEINcoord: 29..472

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25963-RACarg25963-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0009416 response to light stimulus
biological_process GO:0009451 RNA modification
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding