Carg25891 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25891
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	B3 domain-containing protein
Location	Carg_Chr06: 2421078 .. 2423022 (+)
RNA-Seq Expression	Carg25891
Synteny	Carg25891

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTTAAACCACTTCTCTTCAGACTGCCATCTCCACCACCCCAAATCCTCCGCCTCCGCCGTCGACCACCACCTCATCCACCACCACCGCAAGCACCCCATGTTCGAAAAGCCCTTGACTCCCAGCGACGTTGGCAAGCTCAACCGCCTCGTCATCCCCAAGCAATATGCTGAAAAATATTTCCCACTTTCCCCCGGCGGCGACACGGCGGACAAGGGCTTGCTTCTTAGCTTCGAAGATGAGTCCGGTAAGATGTGGCGCTTCCGTTACTCGTATTGGAATAGTAGCCAGAGCTATGTGCTCACTAAAGGGTGGACCCGCTTTGTCAAAGAGAAGCGCCTTGACGCCGGTGATGTCGTCGTCTTTGAACGCCACCGCCGTGATGGGGATAGGTTGTTCATTGGGTGGAAGCGGCGCTCGCCCGTGTCCACGGCGGAGACGGCGGCGGCGACTAGAATCTTCTGTTCTTCAAACCCATATCCTTCTCATCATCATCATCATAATCATAATCATCTTCCTTCACCGCCATACCAATCTTATGATTGTCCTCATGCAGGTATTTTTGTCAAATTTTCACCCTTTTATTTATCCATTAATTACCCAGAAATTAATGTAATATCTTTTATTTTAATTTCTTTATTCCATAAAAAAAGGATTTAAAAAATGAAAGCTGGATGTATGTGCATTGTTTTCAGAGTCCTCTGTTCAGAGTCAAACAGTGTCGGTTGGGAATTCAAAGATACTGCGTCTGTTCGGGGTGGACTTGGAGTGCCAAACCGACATGTCGGAACCCGAACAACCCTCGACGCTCGGTGGCGGATCGGGTCTATCGGGTCAAGATCCAACCCGTCACAGCTTCTACACACACTGACACTATTTCTCATCACATGGTATGACAACTCAACTCCATATTATCACTATTATTATTTTTCCCCACCTTTGAATTTGTTTCATCTAATCAGACAGACAGATTAGAGTCAAAGTGAGATAGGAAAGGAAGAAAAAAAAGAAAAAAGAAAAAAGAAAAAAAGGGAGTGCCATGGGTTATTGTTGCATTTATTAAATGGAATGGTCTCCTCTTTTGGCAACAAAAATCTGTGAATTAATTTAGAAATGTACCTGCAAAGATTGCTCATTTTAATGATATGATTCAATGTCATATGCTTACCCATTTTAAGGACAAAACTATAATTAAATGAAAAGAAATAACATGTGAGTCACATATTGGCTAAAATAAAATGAATATAAACCCACAAAAGATGATTTAGGCTTCTAACCTTGTAGCAATCTGGGATCAATACAAGGTCAACCAGTTCACTATTTTGTAGTCTGTGGTCTATAATAAAGAAAACCCAAATCAACGAATAAGAAAGATAATACAAATTTAGTTTTTTTTTTTTTTTTTTTTTTTTGGAGAGAGAGAGAGAGAGAGAGAGAGTTGAAGAAGAAAAGGAGAAAGGAGATGGAAGGTTTCCAGATTGTAAGGGGAACATCGTACATGGACAGAAGCATCAGAGAAATGATATCGGACCAAAAACTCTGGCACAAATGATATCACACTCTTCCTTTCTTCACTTTCAGAAATCATATGCACAAAAAGTTCAAAGCCAACTCTTCTTATTAGCAGAGCATTACACACTATGGACCAACCAACCCCTTTCAGTTTTTATTTCTACAACACCTCTCTCTCTCTCTCTCTCTCAAGCCTAACAAATCACAGGAAAATCTGCTTGATTATCTACACAAACACACACATATTTGTATTTTACTGTAACAGGTGTGTCATTTTATGGGAGTTCTAAGATGAGGTTGTAACAGGATTCCACTTTCCCACAAGATGTGAAGCAGGCGAAATATGAAGGATAGAGATGTGTGGATGTGACGTCCCTGTAATGCAAAACACACTTTTTCCCACTACACTTGAGAAGAGTCATCCCCACTA

mRNA sequence

ATGTCCTTAAACCACTTCTCTTCAGACTGCCATCTCCACCACCCCAAATCCTCCGCCTCCGCCGTCGACCACCACCTCATCCACCACCACCGCAAGCACCCCATGTTCGAAAAGCCCTTGACTCCCAGCGACGTTGGCAAGCTCAACCGCCTCGTCATCCCCAAGCAATATGCTGAAAAATATTTCCCACTTTCCCCCGGCGGCGACACGGCGGACAAGGGCTTGCTTCTTAGCTTCGAAGATGAGTCCGGTAAGATGTGGCGCTTCCGTTACTCGTATTGGAATAGTAGCCAGAGCTATGTGCTCACTAAAGGGTGGACCCGCTTTGTCAAAGAGAAGCGCCTTGACGCCGGTGATGTCGTCGTCTTTGAACGCCACCGCCGTATTTTTGTCAAATTTTCACCCTTTTATTTATCCATTAATTACCCAGAAATTAATAGTCCTCTGTTCAGAGTCAAACAGTGTCGGTTGGGAATTCAAAGATACTGCGTCTGTTCGGGGTGGACTTGGAGTGCCAAACCGACATGTCGGAACCCGAACAACCCTCGACGCTCGGTGGCGGATCGGGTCTATCGGGTCAAGATCCAACCCGTCACAGCTTCTACACACACTGACACTATTTCTCATCACATGGATTCCACTTTCCCACAAGATGTGAAGCAGGCGAAATATGAAGGATAGAGATGTGTGGATGTGACGTCCCTGTAATGCAAAACACACTTTTTCCCACTACACTTGAGAAGAGTCATCCCCACTA

Coding sequence (CDS)

ATGTCCTTAAACCACTTCTCTTCAGACTGCCATCTCCACCACCCCAAATCCTCCGCCTCCGCCGTCGACCACCACCTCATCCACCACCACCGCAAGCACCCCATGTTCGAAAAGCCCTTGACTCCCAGCGACGTTGGCAAGCTCAACCGCCTCGTCATCCCCAAGCAATATGCTGAAAAATATTTCCCACTTTCCCCCGGCGGCGACACGGCGGACAAGGGCTTGCTTCTTAGCTTCGAAGATGAGTCCGGTAAGATGTGGCGCTTCCGTTACTCGTATTGGAATAGTAGCCAGAGCTATGTGCTCACTAAAGGGTGGACCCGCTTTGTCAAAGAGAAGCGCCTTGACGCCGGTGATGTCGTCGTCTTTGAACGCCACCGCCGTATTTTTGTCAAATTTTCACCCTTTTATTTATCCATTAATTACCCAGAAATTAATAGTCCTCTGTTCAGAGTCAAACAGTGTCGGTTGGGAATTCAAAGATACTGCGTCTGTTCGGGGTGGACTTGGAGTGCCAAACCGACATGTCGGAACCCGAACAACCCTCGACGCTCGGTGGCGGATCGGGTCTATCGGGTCAAGATCCAACCCGTCACAGCTTCTACACACACTGACACTATTTCTCATCACATGGATTCCACTTTCCCACAAGATGTGAAGCAGGCGAAATATGAAGGATAG

Protein sequence

MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDVVVFERHRRIFVKFSPFYLSINYPEINSPLFRVKQCRLGIQRYCVCSGWTWSAKPTCRNPNNPRRSVADRVYRVKIQPVTASTHTDTISHHMDSTFPQDVKQAKYEG

Homology

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BLAST of Carg25891 vs. NCBI nr
Match: KAG7028129.1 (B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 481.9 bits (1239), Expect = 3.1e-132
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRRIFVKFSPFYLSINYPEINSPLFRVKQCRLGIQRYCVCSGWTWSAKPTCRNPN 180
           VVFERHRRIFVKFSPFYLSINYPEINSPLFRVKQCRLGIQRYCVCSGWTWSAKPTCRNPN
Sbjct: 121 VVFERHRRIFVKFSPFYLSINYPEINSPLFRVKQCRLGIQRYCVCSGWTWSAKPTCRNPN 180

Query: 181 NPRRSVADRVYRVKIQPVTASTHTDTISHHMDSTFPQDVKQAKYEG 227
           NPRRSVADRVYRVKIQPVTASTHTDTISHHMDSTFPQDVKQAKYEG
Sbjct: 181 NPRRSVADRVYRVKIQPVTASTHTDTISHHMDSTFPQDVKQAKYEG 226

[Close]

BLAST of Carg25891 vs. NCBI nr
Match: KAG6596592.1 (B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.1 bits (697), Expect = 2.2e-69
Identity = 128/128 (100.00%), Postives = 128/128 (100.00%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRR 129
           VVFERHRR
Sbjct: 121 VVFERHRR 128

[Close]

BLAST of Carg25891 vs. NCBI nr
Match: XP_022938099.1 (B3 domain-containing protein At5g06250-like [Cucurbita moschata] >XP_022938108.1 B3 domain-containing protein At5g06250-like [Cucurbita moschata])

HSP 1 Score: 271.2 bits (692), Expect = 8.3e-69
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHH KHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHHKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRR 129
           VVFERHRR
Sbjct: 121 VVFERHRR 128

[Close]

BLAST of Carg25891 vs. NCBI nr
Match: XP_023005128.1 (B3 domain-containing protein At5g06250-like [Cucurbita maxima] >XP_023005129.1 B3 domain-containing protein At5g06250-like [Cucurbita maxima])

HSP 1 Score: 271.2 bits (692), Expect = 8.3e-69
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHH KHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHHKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRR 129
           VVFERHRR
Sbjct: 121 VVFERHRR 128

[Close]

BLAST of Carg25891 vs. NCBI nr
Match: XP_023539022.1 (B3 domain-containing protein At2g36080-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 266.5 bits (680), Expect = 2.0e-67
Identity = 127/129 (98.45%), Postives = 127/129 (98.45%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLI-HHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAE 60
           MSLNHFSSDCHLHHPKSSASAVDHHLI HHH KHPMFEKPLTPSDVGKLNRLVIPKQYAE
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHHHKHPMFEKPLTPSDVGKLNRLVIPKQYAE 60

Query: 61  KYFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGD 120
           KYFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGD
Sbjct: 61  KYFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGD 120

Query: 121 VVVFERHRR 129
           VVVFERHRR
Sbjct: 121 VVVFERHRR 129

[Close]

BLAST of Carg25891 vs. ExPASy Swiss-Prot
Match: Q53QI0 (B3 domain-containing protein Os11g0156000 OS=Oryza sativa subsp. japonica OX=39947 GN=Os11g0156000 PE=2 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 1.1e-42
Identity = 90/132 (68.18%), Postives = 101/132 (76.52%), Query Frame = 0


Query: 1   MSLNH--FSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYA 60
           M++NH  FS +     P   A   + H  HH+ K  MFEKPLTPSDVGKLNRLVIPKQ+A
Sbjct: 1   MAMNHPLFSQEQPQSWPWGVAMYANFHYHHHYEKEHMFEKPLTPSDVGKLNRLVIPKQHA 60

Query: 61  EKYFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAG 120
           E+YFPL   GD ADKGL+LSFEDE+G  WRFRYSYW SSQSYVLTKGW+R+VKEKRLDAG
Sbjct: 61  ERYFPLG-AGDAADKGLILSFEDEAGAPWRFRYSYWTSSQSYVLTKGWSRYVKEKRLDAG 120

Query: 121 DVVVFERHRRIF 131
           DVV FER R  F
Sbjct: 121 DVVHFERVRGSF 131

[Close]

BLAST of Carg25891 vs. ExPASy Swiss-Prot
Match: Q8GYJ2 (B3 domain-containing protein At2g36080 OS=Arabidopsis thaliana OX=3702 GN=ARF31 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 9.9e-41
Identity = 85/130 (65.38%), Postives = 96/130 (73.85%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHP--KSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYA 60
           MS+N +SSD H H    +       H       K  +FEKPLTPSDVGKLNRLVIPKQ+A
Sbjct: 1   MSINQYSSDFHYHSLMWQQQQQQQQHQNDVVEEKEALFEKPLTPSDVGKLNRLVIPKQHA 60

Query: 61  EKYFPL-SPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDA 120
           E+YFPL +   D  +KGLLL FEDE GK WRFRYSYWNSSQSYVLTKGW+R+VKEK LDA
Sbjct: 61  ERYFPLAAAAADAVEKGLLLCFEDEEGKPWRFRYSYWNSSQSYVLTKGWSRYVKEKHLDA 120

Query: 121 GDVVVFERHR 128
           GDVV+F RHR
Sbjct: 121 GDVVLFHRHR 130

[Close]

BLAST of Carg25891 vs. ExPASy Swiss-Prot
Match: Q9FNI3 (B3 domain-containing protein At5g06250 OS=Arabidopsis thaliana OX=3702 GN=At5g06250 PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 3.8e-40
Identity = 90/153 (58.82%), Postives = 107/153 (69.93%), Query Frame = 0


Query: 1   MSLNHFSSD----------CHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNR 60
           MS+NH+S+D           H H   +S +      +H   K  +FEK LTPSDVGKLNR
Sbjct: 1   MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNR 60

Query: 61  LVIPKQYAEKYFPL------SPGGDT--ADKGLLLSFEDESGKMWRFRYSYWNSSQSYVL 120
           LVIPKQ+AEKYFPL      S   DT  ++KG+LLSFEDESGK WRFRYSYWNSSQSYVL
Sbjct: 61  LVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDESGKSWRFRYSYWNSSQSYVL 120

Query: 121 TKGWTRFVKEKRLDAGDVVVFERH----RRIFV 132
           TKGW+RFVK+K+LD GDVV F+RH    RR+F+
Sbjct: 121 TKGWSRFVKDKQLDPGDVVFFQRHRSDSRRLFI 153

[Close]

BLAST of Carg25891 vs. ExPASy Swiss-Prot
Match: Q8RYD3 (B3 domain-containing protein At3g11580 OS=Arabidopsis thaliana OX=3702 GN=ARF32 PE=2 SV=1)

HSP 1 Score: 165.6 bits (418), Expect = 6.4e-40
Identity = 86/144 (59.72%), Postives = 105/144 (72.92%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MS+NH+ +   LHH   +  A+        ++  +FEK LTPSDVGKLNRLVIPKQ+AEK
Sbjct: 1   MSVNHYHNTLSLHHHHQNDVAI-------AQRESLFEKSLTPSDVGKLNRLVIPKQHAEK 60

Query: 61  YFPLS-------PGGD--TADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVK 120
           YFPL+        G D  T +KG+LLSFEDESGK W+FRYSYWNSSQSYVLTKGW+R+VK
Sbjct: 61  YFPLNNNNNNGGSGDDVATTEKGMLLSFEDESGKCWKFRYSYWNSSQSYVLTKGWSRYVK 120

Query: 121 EKRLDAGDVVVFERHR----RIFV 132
           +K LDAGDVV F+RHR    R+F+
Sbjct: 121 DKHLDAGDVVFFQRHRFDLHRLFI 137

[Close]

BLAST of Carg25891 vs. ExPASy Swiss-Prot
Match: Q6EU30 (B3 domain-containing protein Os02g0683500 OS=Oryza sativa subsp. japonica OX=39947 GN=Os02g0683500 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 4.3e-36
Identity = 72/94 (76.60%), Postives = 80/94 (85.11%), Query Frame = 0


Query: 32  KHPMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLSPGGDTADKGLLLSFEDESGKMWRFRY 91
           K  MF+K +TPSDVGKLNRLVIPKQYAEKYFPL    +  +KGLLL+FED +GK WRFRY
Sbjct: 92  KEHMFDKVVTPSDVGKLNRLVIPKQYAEKYFPLDAAAN--EKGLLLNFEDRAGKPWRFRY 151

Query: 92  SYWNSSQSYVLTKGWTRFVKEKRLDAGDVVVFER 126
           SYWNSSQSYV+TKGW+RFVKEKRLDAGD V F R
Sbjct: 152 SYWNSSQSYVMTKGWSRFVKEKRLDAGDTVSFSR 183

[Close]

BLAST of Carg25891 vs. ExPASy TrEMBL
Match: A0A6J1L1C4 (B3 domain-containing protein At5g06250-like OS=Cucurbita maxima OX=3661 GN=LOC111498261 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.0e-69
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHH KHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHHKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRR 129
           VVFERHRR
Sbjct: 121 VVFERHRR 128

[Close]

BLAST of Carg25891 vs. ExPASy TrEMBL
Match: A0A6J1FHX6 (B3 domain-containing protein At5g06250-like OS=Cucurbita moschata OX=3662 GN=LOC111444287 PE=4 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 4.0e-69
Identity = 127/128 (99.22%), Postives = 127/128 (99.22%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MSLNHFSSDCHLHHPKSSASAVDHHLIHHH KHPMFEKPLTPSDVGKLNRLVIPKQYAEK
Sbjct: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHHKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60

Query: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120
           YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV
Sbjct: 61  YFPLSPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDV 120

Query: 121 VVFERHRR 129
           VVFERHRR
Sbjct: 121 VVFERHRR 128

[Close]

BLAST of Carg25891 vs. ExPASy TrEMBL
Match: A0A6J1F8D4 (B3 domain-containing protein At2g36080-like OS=Cucurbita moschata OX=3662 GN=LOC111441788 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 6.0e-49
Identity = 133/291 (45.70%), Postives = 160/291 (54.98%), Query Frame = 0


Query: 1   MSLNHFSSDCH-------------LHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGK 60
           MS+NHFS D H              ++PKSS S         H K PMFEKPLTPSDVGK
Sbjct: 1   MSINHFSQDFHQPLFSSPHLPIMLSNNPKSSLS--------DHLKQPMFEKPLTPSDVGK 60

Query: 61  LNRLVIPKQYAEKYFPLSPGGD--------TADKGLLLSFEDESGKMWRFRYSYWNSSQS 120
           LNRLVIPKQYAE+YFPLSP           +ADK LLL+FEDESGK+WRFRYSYWNSSQS
Sbjct: 61  LNRLVIPKQYAERYFPLSPSSSSAAAATAHSADKALLLTFEDESGKLWRFRYSYWNSSQS 120

Query: 121 YVLTKGWTRFVKEKRLDAGDVVVFERHR----RIFVKF------SP-------------- 180
           YVLTKGW+RFVKEKRLDAGD VVFERHR    R+F+ +      SP              
Sbjct: 121 YVLTKGWSRFVKEKRLDAGDCVVFERHRSDGDRLFIGWKRRSAPSPAETAVAPSGSGGGC 180

Query: 181 ----FYLSINYP---EINSPLFRVKQCRL--GIQRYCVCSGWT-----WSAKPTCR---- 227
               FY +  YP    + SP ++     L   +Q   V  G +     +     C+    
Sbjct: 181 WTRMFYSANPYPSHHHLPSPPYQSSGSTLPESVQSQTVSVGNSKILRLFGVDLECQTDMS 240

[Close]

BLAST of Carg25891 vs. ExPASy TrEMBL
Match: A0A6J1IY80 (B3 domain-containing protein At2g36080-like OS=Cucurbita maxima OX=3661 GN=LOC111481716 PE=4 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 3.0e-48
Identity = 132/292 (45.21%), Postives = 160/292 (54.79%), Query Frame = 0


Query: 1   MSLNHFSSDCH-------------LHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGK 60
           MS+NHFS D H              ++PKSS S         + K PMFEKPLTPSDVGK
Sbjct: 1   MSINHFSQDFHQPLFSSPHLPIMLSNNPKSSLS--------DNLKQPMFEKPLTPSDVGK 60

Query: 61  LNRLVIPKQYAEKYFPLSPGGD--------TADKGLLLSFEDESGKMWRFRYSYWNSSQS 120
           LNRLVIPKQYAE+YFPLSP           +ADK LLL+FEDESGK+WRFRYSYWNSSQS
Sbjct: 61  LNRLVIPKQYAERYFPLSPSSSAAAAATAHSADKALLLTFEDESGKLWRFRYSYWNSSQS 120

Query: 121 YVLTKGWTRFVKEKRLDAGDVVVFERHR----RIFVKF------SP-------------- 180
           YVLTKGW+RFVKEKRLDAGD VVFERHR    R+F+ +      SP              
Sbjct: 121 YVLTKGWSRFVKEKRLDAGDCVVFERHRSDGDRLFIGWKRRSAPSPAETAVAPSGSGGGC 180

Query: 181 ----FYLSINYP---EINSPLFRVKQCRL---GIQRYCVCSGWT-----WSAKPTCR--- 227
               FY +  YP    + SP ++     L    +Q   V  G +     +     C+   
Sbjct: 181 WTRMFYSANPYPSHHHLPSPPYQSSGSTLQESSVQSQTVSVGNSKILRLFGVDLECQTDM 240

[Close]

BLAST of Carg25891 vs. ExPASy TrEMBL
Match: A0A6J1CU42 (B3 domain-containing protein At2g36080 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014360 PE=4 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 2.8e-46
Identity = 126/265 (47.55%), Postives = 142/265 (53.58%), Query Frame = 0


Query: 12  LHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLSPGGDT- 71
           L H  S+ SA DH         PMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLS    T 
Sbjct: 12  LRHQPSAMSAADH--------QPMFEKPLTPSDVGKLNRLVIPKQYAEKYFPLSGAAATA 71

Query: 72  ADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDAGDVVVFERHR--- 131
           ADKGLLLSFEDESGK+WRFRYSYWNSSQSYVLTKGW+R+VKEKRLDAGDVVVFERHR   
Sbjct: 72  ADKGLLLSFEDESGKLWRFRYSYWNSSQSYVLTKGWSRYVKEKRLDAGDVVVFERHRLDG 131

Query: 132 -RIFV---KFSPFYLSI----NYPEINSPLFRVKQCRLGIQRYCVCSGWTWSAKPTCRNP 191
            R+F+   + SP   +       P +  P      C  G      CSG  +   P     
Sbjct: 132 DRLFIGWKRRSPAAAAAADGGRSPGVAPP------CSRG-WTSMFCSGHPYHPSPPPYQS 191

Query: 192 NNPRRSVADRVYRV------KIQPVTASTHTDT--------------------------- 225
           ++P  SV  R   V      ++  V     T+                            
Sbjct: 192 SHPESSVQSRTVSVGNSKILRLFGVNLECQTNVSEPEPPPSGGGGGFDLSGRNDPTRHHS 251

[Close]

BLAST of Carg25891 vs. TAIR 10
Match: AT2G36080.2 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 168.3 bits (425), Expect = 7.0e-42
Identity = 85/130 (65.38%), Postives = 96/130 (73.85%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHP--KSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYA 60
           MS+N +SSD H H    +       H       K  +FEKPLTPSDVGKLNRLVIPKQ+A
Sbjct: 1   MSINQYSSDFHYHSLMWQQQQQQQQHQNDVVEEKEALFEKPLTPSDVGKLNRLVIPKQHA 60

Query: 61  EKYFPL-SPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDA 120
           E+YFPL +   D  +KGLLL FEDE GK WRFRYSYWNSSQSYVLTKGW+R+VKEK LDA
Sbjct: 61  ERYFPLAAAAADAVEKGLLLCFEDEEGKPWRFRYSYWNSSQSYVLTKGWSRYVKEKHLDA 120

Query: 121 GDVVVFERHR 128
           GDVV+F RHR
Sbjct: 121 GDVVLFHRHR 130

[Close]

BLAST of Carg25891 vs. TAIR 10
Match: AT2G36080.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 168.3 bits (425), Expect = 7.0e-42
Identity = 85/130 (65.38%), Postives = 96/130 (73.85%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHP--KSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYA 60
           MS+N +SSD H H    +       H       K  +FEKPLTPSDVGKLNRLVIPKQ+A
Sbjct: 1   MSINQYSSDFHYHSLMWQQQQQQQQHQNDVVEEKEALFEKPLTPSDVGKLNRLVIPKQHA 60

Query: 61  EKYFPL-SPGGDTADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVKEKRLDA 120
           E+YFPL +   D  +KGLLL FEDE GK WRFRYSYWNSSQSYVLTKGW+R+VKEK LDA
Sbjct: 61  ERYFPLAAAAADAVEKGLLLCFEDEEGKPWRFRYSYWNSSQSYVLTKGWSRYVKEKHLDA 120

Query: 121 GDVVVFERHR 128
           GDVV+F RHR
Sbjct: 121 GDVVLFHRHR 130

[Close]

BLAST of Carg25891 vs. TAIR 10
Match: AT5G06250.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.7e-41
Identity = 90/153 (58.82%), Postives = 107/153 (69.93%), Query Frame = 0


Query: 1   MSLNHFSSD----------CHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNR 60
           MS+NH+S+D           H H   +S +      +H   K  +FEK LTPSDVGKLNR
Sbjct: 1   MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNR 60

Query: 61  LVIPKQYAEKYFPL------SPGGDT--ADKGLLLSFEDESGKMWRFRYSYWNSSQSYVL 120
           LVIPKQ+AEKYFPL      S   DT  ++KG+LLSFEDESGK WRFRYSYWNSSQSYVL
Sbjct: 61  LVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDESGKSWRFRYSYWNSSQSYVL 120

Query: 121 TKGWTRFVKEKRLDAGDVVVFERH----RRIFV 132
           TKGW+RFVK+K+LD GDVV F+RH    RR+F+
Sbjct: 121 TKGWSRFVKDKQLDPGDVVFFQRHRSDSRRLFI 153

[Close]

BLAST of Carg25891 vs. TAIR 10
Match: AT5G06250.2 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 166.4 bits (420), Expect = 2.7e-41
Identity = 90/153 (58.82%), Postives = 107/153 (69.93%), Query Frame = 0


Query: 1   MSLNHFSSD----------CHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNR 60
           MS+NH+S+D           H H   +S +      +H   K  +FEK LTPSDVGKLNR
Sbjct: 1   MSVNHYSTDHHHTLLWQQQQHRHTTDTSETTTTATWLHDDLKESLFEKSLTPSDVGKLNR 60

Query: 61  LVIPKQYAEKYFPL------SPGGDT--ADKGLLLSFEDESGKMWRFRYSYWNSSQSYVL 120
           LVIPKQ+AEKYFPL      S   DT  ++KG+LLSFEDESGK WRFRYSYWNSSQSYVL
Sbjct: 61  LVIPKQHAEKYFPLNAVLVSSAAADTSSSEKGMLLSFEDESGKSWRFRYSYWNSSQSYVL 120

Query: 121 TKGWTRFVKEKRLDAGDVVVFERH----RRIFV 132
           TKGW+RFVK+K+LD GDVV F+RH    RR+F+
Sbjct: 121 TKGWSRFVKDKQLDPGDVVFFQRHRSDSRRLFI 153

[Close]

BLAST of Carg25891 vs. TAIR 10
Match: AT3G11580.1 (AP2/B3-like transcriptional factor family protein )

HSP 1 Score: 165.6 bits (418), Expect = 4.5e-41
Identity = 86/144 (59.72%), Postives = 105/144 (72.92%), Query Frame = 0


Query: 1   MSLNHFSSDCHLHHPKSSASAVDHHLIHHHRKHPMFEKPLTPSDVGKLNRLVIPKQYAEK 60
           MS+NH+ +   LHH   +  A+        ++  +FEK LTPSDVGKLNRLVIPKQ+AEK
Sbjct: 1   MSVNHYHNTLSLHHHHQNDVAI-------AQRESLFEKSLTPSDVGKLNRLVIPKQHAEK 60

Query: 61  YFPLS-------PGGD--TADKGLLLSFEDESGKMWRFRYSYWNSSQSYVLTKGWTRFVK 120
           YFPL+        G D  T +KG+LLSFEDESGK W+FRYSYWNSSQSYVLTKGW+R+VK
Sbjct: 61  YFPLNNNNNNGGSGDDVATTEKGMLLSFEDESGKCWKFRYSYWNSSQSYVLTKGWSRYVK 120

Query: 121 EKRLDAGDVVVFERHR----RIFV 132
           +K LDAGDVV F+RHR    R+F+
Sbjct: 121 DKHLDAGDVVFFQRHRFDLHRLFI 137

The following BLAST results are available for this feature:

BLAST of Carg25891 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028129.1	3.1e-132	100.00	B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
KAG6596592.1	2.2e-69	100.00	B3 domain-containing protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022938099.1	8.3e-69	99.22	B3 domain-containing protein At5g06250-like [Cucurbita moschata] >XP_022938108.1...	[more]
XP_023005128.1	8.3e-69	99.22	B3 domain-containing protein At5g06250-like [Cucurbita maxima] >XP_023005129.1 B...	[more]
XP_023539022.1	2.0e-67	98.45	B3 domain-containing protein At2g36080-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg25891 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q53QI0	1.1e-42	68.18	B3 domain-containing protein Os11g0156000 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q8GYJ2	9.9e-41	65.38	B3 domain-containing protein At2g36080 OS=Arabidopsis thaliana OX=3702 GN=ARF31 ...	[more]
Q9FNI3	3.8e-40	58.82	B3 domain-containing protein At5g06250 OS=Arabidopsis thaliana OX=3702 GN=At5g06...	[more]
Q8RYD3	6.4e-40	59.72	B3 domain-containing protein At3g11580 OS=Arabidopsis thaliana OX=3702 GN=ARF32 ...	[more]
Q6EU30	4.3e-36	76.60	B3 domain-containing protein Os02g0683500 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of Carg25891 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L1C4	4.0e-69	99.22	B3 domain-containing protein At5g06250-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1FHX6	4.0e-69	99.22	B3 domain-containing protein At5g06250-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1F8D4	6.0e-49	45.70	B3 domain-containing protein At2g36080-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IY80	3.0e-48	45.21	B3 domain-containing protein At2g36080-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1CU42	2.8e-46	47.55	B3 domain-containing protein At2g36080 isoform X1 OS=Momordica charantia OX=3673...	[more]

BLAST of Carg25891 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G36080.2	7.0e-42	65.38	AP2/B3-like transcriptional factor family protein	[more]
AT2G36080.1	7.0e-42	65.38	AP2/B3-like transcriptional factor family protein	[more]
AT5G06250.1	2.7e-41	58.82	AP2/B3-like transcriptional factor family protein	[more]
AT5G06250.2	2.7e-41	58.82	AP2/B3-like transcriptional factor family protein	[more]
AT3G11580.1	4.5e-41	59.72	AP2/B3-like transcriptional factor family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003340	B3 DNA binding domain	SMART	SM01019	B3_2	coord: 36..139 e-value: 2.9E-27 score: 106.6
IPR003340	B3 DNA binding domain	PFAM	PF02362	B3	coord: 36..127 e-value: 6.7E-29 score: 99.7
IPR003340	B3 DNA binding domain	PROSITE	PS50863	B3	coord: 36..144 score: 15.171043
IPR003340	B3 DNA binding domain	CDD	cd10017	B3_DNA	coord: 34..128 e-value: 1.87493E-31 score: 108.954
IPR015300	DNA-binding pseudobarrel domain superfamily	GENE3D	2.40.330.10		coord: 30..135 e-value: 8.6E-43 score: 146.8
IPR015300	DNA-binding pseudobarrel domain superfamily	SUPERFAMILY	101936	DNA-binding pseudobarrel domain	coord: 31..132
IPR044800	B3 domain-containing transcription factor LEC2-like	PANTHER	PTHR31140	B3 DOMAIN-CONTAINING TRANSCRIPTION FACTOR ABI3	coord: 20..131
None	No IPR available	PANTHER	PTHR31140:SF70	B3 DOMAIN-CONTAINING PROTEIN OS11G0156000	coord: 20..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25891-RA	Carg25891-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity