Carg25846 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25846
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein NRT1/ PTR FAMILY 4.5-like
Location	Carg_Chr12: 3033492 .. 3036744 (-)
RNA-Seq Expression	Carg25846
Synteny	Carg25846

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTGGATTAGGTGCTAAAAATGGGGACAAAAAGGGACCCAAACGAGGGGGAAACAGAGCTGCTGGTTTTATTTATGGTACTGTGTGCTTATATGCTTTGGTTTTAGAGTATGGATTTGAGTAATTGGTTTATTTGATTGATTGATTTGGGGATTACAGTGACTGAAGGACTGGAAAATATGGCGTTTGTGGCGTTGGCCTTGAGCTTGATGACATATTTCAATGGGTATATGAACTTCAGCTTGACAAAATCGGCTACCACTTTGACCAACTTCATGGGAACTACGTTCCTTCTCACGCTCGTCGGCGGCTTTGTCTCCGACACGTATCTCTCCCGATTCAAAACCTGCGTCCTCTTCGCTTGCTTTGAGTTACTGGTTAGTGCCTCATTCTAGTATCGAGCCCACTGCTAACAGATGTTGATGTGTATTGTCCTTTTCTGGGCTTTTTCTTTTGTGTTTTCTCTCGACTTTTTTAAAACTCGTGTGTTATGGAGAGGTTTCGACATCTTTATAAAGGGTGTTTCGTTCTCCTTCCCAACCGATGTGGGATCTCACAATCCACCCTCTTTCGAGGCTAGCGTTCTCTCTGGCACTCGTTCCCTTCTTCAATCGATGTGAGACCCCCTAATCCACCCCTCCTTTGAGGCTTAGTGTCCTTACTGGCACACCGCCTCATGTCCACCCTCCTTCAGGGCTTAGCCTCCTCACTGGCACAGCGCTCAGTGTCTACCTTTGATATCATTAGTAACTGTCCAAGCCCACCGCTAGTAGATATTGTCTACTTTGGACTTTTCTACCGGTTCTCTCCAATGTTTTTAAAATGCATATGCTAGGGAGCGGTTTACACACTTTTATAAAAATGTTTCATTCTCGTTATAGATGTGTGATCTCACAATTGTCTTTTGTTCTTAAAAAACGTAAATGTTTTTGTTGTCTAAGATAGTTAAAGAGCTTGAATTTTTTAATTTTAGTGTCATTTTTTATACAAGTTGCTACCCGTGTAGAAACAGGAGATTTGAACCACTAAATCTTTGTGTCTCTTTGTTTAATTTTTGAAGGGATATTGTCTACTAACGGCACAGGCGCATTTCCGGGAGCTAAGGCCAGAGCCGTGCAAAGGGGCGGCGGCGGGGAGTGAGTGCAAGGCGGCGAACGGCGGGCAGGAGGCGATTCTGTACGTGGGAATTTACCTGATAGCGCTGGGAGCGAGTGGGGTAAAAGTGGCGCTGCCGGCGTTGGGAGCGGATCAGTTCGAGGAGGGAGATCCGGCGGAGGCGGGGAAACTGTCGAGCTTTTTCAACTGGTTCTTGTTCAGCCTGACCACAGGGTCGACCGTGGGGTTGACATTGATAGTGTGGATAAACACGGAGGTGGGCTGGGACTGGGCGTTTGTGGTGTGCACCACCGCCGTTCTCGCCGCCATTCTTGTTGTGTGCGCCGGCAAGTCCTTCTTTCGCAATAATGTGCCTAAAGGAAGCCCCATTCTTAGAATTCTACAGGTATCTTCATTTTCCTCCATTTAAATCTCAAATATCTCTATTTTTTTTTTTATTATTAATTTTTAATTTGAGTTCGTGGAAAGTAAAATAAAATATTTAGACTTACAATACTTTAATTATGAAGTTATCTCGACCATTAATATATAGTTAAAAATCCATTTTATCTTATATATAATGGGTGTTCATATGACTCGATAACTCGATCAACTTAAACTACTTAATTTAAACCATAAAGGTTGGGTTGAGTTAGATTTTTTTTAGATCCCCTACCAAATTTATATATTGTGATTGTGACAAGTGGTTGGGTTGAATTCAGTTGAGTTTAGGGTGATATCAGTTGACCCAAACCGAAAATAGAGTTACTATCCAATCCTATGAACTTTAGTCATATTGCTCGAATCACCAACTCAATTGACTCGATTCAAAAGATTTTTACAACCAAACCCATTTATATCCCTACTCTAAACTTGGATGGTCGTATACTCTAAACTTTTATTTTCATCACGTTTCAATGAATCATAAACTAAAAACTAAATGTATCAAATTGTTCATATTTTTCTGAGTTAATTTTCAATTTTATCATATGGGTCTCCTTAAATGCTATTAAAATCATTCCAAACACGTTCCTAATCTTATTTTATCATCATCATTCATTGTGTAATGAATGTTTGTTGAATTTTCTTCTTATGATGCATAGGTCATTGTAGCTTCAATCCGTAACAGGAAACTTCCACTTCCAAATAATGCTGATGAACTACATGAGATTCGTGGCAAAGAAGCAGATATCCCAATTGAAATCCTTGAGAAAACCGATCAATTCAGGTGTGATATCTTTGAAAATCTTTCTCTAAAAACAGCTAATGGTTTGGAATTCAATATAATATCGAAGGAAAATGGTGAAATTTTATAGATTCTTAGAACGAGCAGCAATTATAAGGAATGATACAAGAACACCATGGAGACTCTGCACAGTGACACAGGTTGAAGAGACAAAGATTCTAATTCGAATGCTCCCGATCATAGTCAGCACCATTTTCATGGCTACCTGCATGGCTCAACTCCAGACATTCTCCATTCAACAAAGCTTAACTATGGACTGCAATTTCATAGGATTCAAAATTCCCGGCCCCTCCATCCCTGTTATCCCCTTGCTCTTCATGTTCTTCTTCATCCCATTTTACGAGCGCGTTTTTGTTCCTCTGGCCCGTAAAATCACTGGAATTCCCACGGGAATTCGCCACCTCCAAAGGATTGGTTTCGGACTGATACTCACTGCAGCATCCATGGCCATTGCTGGGTTCGTGGAGACCGACGCAAGAACGTCGCGATTCAACACAACATGGTGGATAGTGAGGAGCCTCTACCAATGAGTGTGTTTTGGTTGTCATTTCAGTTCTGTGTATTTGGAATGGCTGATATATTCACTATGGTAGGTCTGCTGGAATTCTTTTACGCCGAGAGCTCGGCAGAGATGAAATCGTTGAGCACAGCCATCGCTTGGTGCTCAATCGCTTTCGGTTACTTCACGAGTACGGTGGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGGTGGTTGGCGAGCAACAACCTCAACAGGGACAATTTGAACTACTTCTACTGGTTACTGTCAGTGTTGAGTGTGGTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTGGAGGTCCTACAGAAACAAGGTGAAGAAGGGTTTGACATAGTGCAGGGGCCCTAA

mRNA sequence

ATGTGTGGATTAGGTGCTAAAAATGGGGACAAAAAGGGACCCAAACGAGGGGGAAACAGAGCTGCTGTGACTGAAGGACTGGAAAATATGGCGTTTGTGGCGTTGGCCTTGAGCTTGATGACATATTTCAATGGGTATATGAACTTCAGCTTGACAAAATCGGCTACCACTTTGACCAACTTCATGGGAACTACGTTCCTTCTCACGCTCGTCGGCGGCTTTGTCTCCGACACTGAGTGCAAGGCGGCGAACGGCGGGCAGGAGGCGATTCTGTACGTGGGAATTTACCTGATAGCGCTGGGAGCGAGTGGGGTAAAAGTGGCGCTGCCGGCGTTGGGAGCGGATCAGTTCGAGGAGGGAGATCCGGCGGAGGCGGGGAAACTGTCGAGCTTTTTCAACTGGTTCTTGTTCAGCCTGACCACAGGGTCGACCGTGGGGTTGACATTGATAGTGTGGATAAACACGGAGGTGGGCTGGGACTGGGCGTTTGTGGTGTGCACCACCGCCGTTCTCGCCGCCATTCTTGTTGTGTGCGCCGGCAAGTCCTTCTTTCGCAATAATGTGCCTAAAGGAAGCCCCATTCTTAGAATTCTACAGGTCATTGTAGCTTCAATCCGTAACAGGAAACTTCCACTTCCAAATAATGCTGATGAACTACATGAGATTCGTGGCAAAGAAGCAGATATCCCAATTGAAATCCTTGAGAAAACCGATCAATTCAGCTTAACTATGGACTGCAATTTCATAGGATTCAAAATTCCCGGCCCCTCCATCCCTGTTATCCCCTTGCTCTTCATGTTCTTCTTCATCCCATTTTACGAGCGCGTTTTTGTTCCTCTGGCCCGTAAAATCACTGGAATTCCCACGGGAATTCGCCACCTCCAAAGGATTGGTTTCGGACTGATACTCACTGCAGCATCCATGGCCATTGCTGGGTTCGTGGAGACCGACGCAAGAACGTCGCGATTCAACACAACATGGTGGATATTCTGTGTATTTGGAATGGCTGATATATTCACTATGGTAGGTCTGCTGGAATTCTTTTACGCCGAGAGCTCGGCAGAGATGAAATCGTTGAGCACAGCCATCGCTTGGTGCTCAATCGCTTTCGGTTACTTCACGAGTACGGTGGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGGTGGTTGGCGAGCAACAACCTCAACAGGGACAATTTGAACTACTTCTACTGGTTACTGTCAGTGTTGAGTGTGGTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTGGAGGTCCTACAGAAACAAGGTGAAGAAGGGTTTGACATAGTGCAGGGGCCCTAA

Coding sequence (CDS)

ATGTGTGGATTAGGTGCTAAAAATGGGGACAAAAAGGGACCCAAACGAGGGGGAAACAGAGCTGCTGTGACTGAAGGACTGGAAAATATGGCGTTTGTGGCGTTGGCCTTGAGCTTGATGACATATTTCAATGGGTATATGAACTTCAGCTTGACAAAATCGGCTACCACTTTGACCAACTTCATGGGAACTACGTTCCTTCTCACGCTCGTCGGCGGCTTTGTCTCCGACACTGAGTGCAAGGCGGCGAACGGCGGGCAGGAGGCGATTCTGTACGTGGGAATTTACCTGATAGCGCTGGGAGCGAGTGGGGTAAAAGTGGCGCTGCCGGCGTTGGGAGCGGATCAGTTCGAGGAGGGAGATCCGGCGGAGGCGGGGAAACTGTCGAGCTTTTTCAACTGGTTCTTGTTCAGCCTGACCACAGGGTCGACCGTGGGGTTGACATTGATAGTGTGGATAAACACGGAGGTGGGCTGGGACTGGGCGTTTGTGGTGTGCACCACCGCCGTTCTCGCCGCCATTCTTGTTGTGTGCGCCGGCAAGTCCTTCTTTCGCAATAATGTGCCTAAAGGAAGCCCCATTCTTAGAATTCTACAGGTCATTGTAGCTTCAATCCGTAACAGGAAACTTCCACTTCCAAATAATGCTGATGAACTACATGAGATTCGTGGCAAAGAAGCAGATATCCCAATTGAAATCCTTGAGAAAACCGATCAATTCAGCTTAACTATGGACTGCAATTTCATAGGATTCAAAATTCCCGGCCCCTCCATCCCTGTTATCCCCTTGCTCTTCATGTTCTTCTTCATCCCATTTTACGAGCGCGTTTTTGTTCCTCTGGCCCGTAAAATCACTGGAATTCCCACGGGAATTCGCCACCTCCAAAGGATTGGTTTCGGACTGATACTCACTGCAGCATCCATGGCCATTGCTGGGTTCGTGGAGACCGACGCAAGAACGTCGCGATTCAACACAACATGGTGGATATTCTGTGTATTTGGAATGGCTGATATATTCACTATGGTAGGTCTGCTGGAATTCTTTTACGCCGAGAGCTCGGCAGAGATGAAATCGTTGAGCACAGCCATCGCTTGGTGCTCAATCGCTTTCGGTTACTTCACGAGTACGGTGGTGGTGGAGGTGGTGAACAAGGCGAGCGGAGGGTGGTTGGCGAGCAACAACCTCAACAGGGACAATTTGAACTACTTCTACTGGTTACTGTCAGTGTTGAGTGTGGTGAATTTTGGGTTTTATTTGGTTTGTGCTTCTTGGTATAGGTATAAGAATGTGGAGGTCCTACAGAAACAAGGTGAAGAAGGGTTTGACATAGTGCAGGGGCCCTAA

Protein sequence

MCGLGAKNGDKKGPKRGGNRAAVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDTECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQFSLTMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDARTSRFNTTWWIFCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLVCASWYRYKNVEVLQKQGEEGFDIVQGP

Homology

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BLAST of Carg25846 vs. NCBI nr
Match: KAG7020549.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 885.6 bits (2287), Expect = 1.8e-253
Identity = 447/447 (100.00%), Postives = 447/447 (100.00%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAAVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTN 60
           MCGLGAKNGDKKGPKRGGNRAAVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTN
Sbjct: 1   MCGLGAKNGDKKGPKRGGNRAAVTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTN 60

Query: 61  FMGTTFLLTLVGGFVSDTECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEG 120
           FMGTTFLLTLVGGFVSDTECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEG
Sbjct: 61  FMGTTFLLTLVGGFVSDTECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEG 120

Query: 121 DPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAG 180
           DPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAG
Sbjct: 121 DPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAG 180

Query: 181 KSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF 240
           KSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF
Sbjct: 181 KSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF 240

Query: 241 SLTMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFG 300
           SLTMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFG
Sbjct: 241 SLTMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFG 300

Query: 301 LILTAASMAIAGFVETDARTSRFNTTWWIFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 360
           LILTAASMAIAGFVETDARTSRFNTTWWIFCVFGMADIFTMVGLLEFFYAESSAEMKSLS
Sbjct: 301 LILTAASMAIAGFVETDARTSRFNTTWWIFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 360

Query: 361 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLV 420
           TAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLV
Sbjct: 361 TAIAWCSIAFGYFTSTVVVEVVNKASGGWLASNNLNRDNLNYFYWLLSVLSVVNFGFYLV 420

Query: 421 CASWYRYKNVEVLQKQGEEGFDIVQGP 448
           CASWYRYKNVEVLQKQGEEGFDIVQGP
Sbjct: 421 CASWYRYKNVEVLQKQGEEGFDIVQGP 447

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BLAST of Carg25846 vs. NCBI nr
Match: XP_022951878.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 784.3 bits (2024), Expect = 5.7e-223
Identity = 432/567 (76.19%), Postives = 437/567 (77.07%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           MCGLGAKNGDKKGPKRGGNRAA    VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 1   MCGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           TLTNFMGTTFLLTLVGGFVSDT                                      
Sbjct: 61  TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYCLLTAQAHFRELRPEPCKG 120

Query: 121 -----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180
                ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 121 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180

Query: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSP 240
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCAGKSFFRNNVPKGSP 240

Query: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF------------- 300
           ILRILQVIVASIRNRKLPLPNNADELHEIR KEADIPIEILEKTDQF             
Sbjct: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 300

Query: 301 ---------------------------------------------SLTMDCNFIGFKIPG 360
                                                        SLTMD NFIGFKIPG
Sbjct: 301 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPG 360

Query: 361 PSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420
           PS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 361 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420

Query: 421 T------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLS 448
           T            D+      + +W+   FCVFGMADIFTMVGLLEFFYAESSAEMKSLS
Sbjct: 421 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 480

[Close]

BLAST of Carg25846 vs. NCBI nr
Match: KAG6585639.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 783.1 bits (2021), Expect = 1.3e-222
Identity = 429/559 (76.74%), Postives = 435/559 (77.82%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           MCGLGAKNGDKKGPKRGGNRAA    V EGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 250 MCGLGAKNGDKKGPKRGGNRAAGFIYVIEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 309

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-----------------------------------ECK 120
           TLTNFMGTTFLLTLVGGFVSDT                                   ECK
Sbjct: 310 TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLAHFRELRPEPCKGTAAGSECK 369

Query: 121 AANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTT 180
           AANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTT
Sbjct: 370 AANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTT 429

Query: 181 GSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVI 240
           GSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVC+GKSFFRNNVPKGSPILRILQVI
Sbjct: 430 GSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCSGKSFFRNNVPKGSPILRILQVI 489

Query: 241 VASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF--------------------- 300
           VASIRNRKLPLPNN DELHEIRGKEADIPIEILEKTDQF                     
Sbjct: 490 VASIRNRKLPLPNNVDELHEIRGKEADIPIEILEKTDQFRFLERAAIIRNDTRTPWRLCT 549

Query: 301 -------------------------------------SLTMDCNFIGFKIPGPSIPVIPL 360
                                                SLTMDCNFIGFKIPGPS+PVIPL
Sbjct: 550 VTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDCNFIGFKIPGPSVPVIPL 609

Query: 361 LFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVET------- 420
           LFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVET       
Sbjct: 610 LFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETRRKNVAI 669

Query: 421 -----DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSI 448
                D+      + +W+   FCVFGMADIFTMVGLLEFFYAESSA MKSLSTAIAWCSI
Sbjct: 670 QHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLSTAIAWCSI 729

[Close]

BLAST of Carg25846 vs. NCBI nr
Match: XP_023002887.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 769.2 bits (1985), Expect = 1.9e-218
Identity = 424/566 (74.91%), Postives = 433/566 (76.50%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           +CGLGAKNGDKKGPKRGGNRAA    VTEGLENMAFVALALSLMTYF+GYMNFSLTKSAT
Sbjct: 16  ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFSGYMNFSLTKSAT 75

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           T+TNFMGTTFLLTLVGGFVSDT                                      
Sbjct: 76  TMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYFLLSAQAHFRELRPEPCKG 135

Query: 121 -----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180
                ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 136 ALAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 195

Query: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSP 240
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 196 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 255

Query: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF------------- 300
           ILRILQVIVASI NRKLPLP NADELHEIR KEADIPIEILEKTDQF             
Sbjct: 256 ILRILQVIVASIHNRKLPLPENADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 315

Query: 301 ---------------------------------------------SLTMDCNFIGFKIPG 360
                                                        SLTMD NF+GFKIPG
Sbjct: 316 RTLWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPG 375

Query: 361 PSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420
           PS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 376 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 435

Query: 421 T------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLS 447
           T            D+      + +W+   FCVFGMADIFTMVGLLEFFYAESSA MKSLS
Sbjct: 436 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLS 495

[Close]

BLAST of Carg25846 vs. NCBI nr
Match: XP_023537870.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 763.8 bits (1971), Expect = 8.0e-217
Identity = 422/567 (74.43%), Postives = 432/567 (76.19%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           +CGLGAKNGDKKGPKRGGNRAA    VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 17  ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 76

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           TLTNFMGTTFLLTLVGGFVSDT                                      
Sbjct: 77  TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVVFACFELLGYCLLTAQAHFRELRPKPCKG 136

Query: 121 -----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180
                ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 137 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 196

Query: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSP 240
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 197 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 256

Query: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF------------- 300
           ILRILQVIVASI NRKLPLP+NADELHEI  KEADIPIEILEKTDQF             
Sbjct: 257 ILRILQVIVASIHNRKLPLPDNADELHEICDKEADIPIEILEKTDQFRFLERAAIIRNDT 316

Query: 301 ---------------------------------------------SLTMDCNFIGFKIPG 360
                                                        SLTMD NF+GFKIPG
Sbjct: 317 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPG 376

Query: 361 PSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420
           PS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 377 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 436

Query: 421 T------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLS 448
           T            D++     + +W+   FCVFGMADIFTMVGLLEFFYAESSA MKSLS
Sbjct: 437 TRRKNVAIQHNMVDSQEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLS 496

[Close]

BLAST of Carg25846 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 5.1e-73
Identity = 182/556 (32.73%), Postives = 276/556 (49.64%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA    V E LEN+A++A A +L+ Y   YM+ S +KSA  +TNFMGT FLL L
Sbjct: 25  RHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLAL 84

Query: 75  VGGFVSDT---------------------------------------ECKAANGGQEAIL 134
           +GGF+SD                                         C+  +G + A+L
Sbjct: 85  LGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAML 144

Query: 135 YVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIV 194
           +VG+YL+ALG  G+K +L + GA+QF+E  P    + S+FFN+F+F L  G+ V +T +V
Sbjct: 145 FVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVV 204

Query: 195 WINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIRNRKL 254
           W+    GW+W F V T A+  +IL+  +G  F+RN +P GSP+  IL+V++ AS++    
Sbjct: 205 WLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSS 264

Query: 255 PLPNNA-----------------------DELHEIRGKEA-------------------- 314
              +NA                        EL + R +EA                    
Sbjct: 265 GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADE 324

Query: 315 ---------------DIPIEI---------------LEKTDQFSL----TMDCNFIGFKI 374
                          D+ I +               L +   FS+    +M+      KI
Sbjct: 325 KPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKI 384

Query: 375 PGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 430
           P  S+P+ P++F+    P Y+ + +P ARK T   TG+ HLQRIG GL+L+  +MA+A  
Sbjct: 385 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 444

[Close]

BLAST of Carg25846 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 6.7e-73
Identity = 180/535 (33.64%), Postives = 271/535 (50.65%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA      E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 27  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 86

Query: 75  VGGFVSDT------------------------------------------ECKAANGGQE 134
           +GGF++D                                            C+   G + 
Sbjct: 87  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKA 146

Query: 135 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 194
           A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +T
Sbjct: 147 AFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVT 206

Query: 195 LIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVA----- 254
            +VWI    GW+W F V T ++  +ILV   G  F++N +P+GSP+  I +V++A     
Sbjct: 207 FVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS 266

Query: 255 ----------------SIRNRKLP---LPNNADELHE-IRGKEADIPIE----------- 314
                           S    K P   L N+   L++ I GK   I +E           
Sbjct: 267 CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKI 326

Query: 315 ----------------ILEKTDQFSL----TMDCNFIGFKIPGPSIPVIPLLFMFFFIPF 374
                            L +   +S+    TM+   + F +P  S+PV P++FM    P 
Sbjct: 327 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPT 386

Query: 375 YERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE------------TDART 428
           Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE             D+  
Sbjct: 387 YDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEE 446

[Close]

BLAST of Carg25846 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.5e-69
Identity = 186/533 (34.90%), Postives = 263/533 (49.34%), Query Frame = 0


Query: 15  KRGGNRAAV----TEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           K GG RAA+     +  E MA  A+  +L+TY    M+F L+KSA  +TNF+GT FLL+L
Sbjct: 38  KHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSL 97

Query: 75  VGGFVSDT-------------------------------------------ECKAANGGQ 134
           +GGF+SD+                                            C  ANG +
Sbjct: 98  LGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYK 157

Query: 135 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 194
            A LY  + L+ALG+  +K  + + GA+QF+  D     KLSSFFN   F+ + G  + L
Sbjct: 158 AATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIAL 217

Query: 195 TLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRN 254
           TL+VW+ T  G D  F V    + A ++ + AG SF+RN  P GS    I QV VA+I  
Sbjct: 218 TLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITK 277

Query: 255 RKLPLPNNADELHE--------------------------IRGKEADIPIEI--LEKTDQ 314
           RK   P+N + +H+                           +GK  + P  +  +E+  Q
Sbjct: 278 RKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQ 337

Query: 315 ----------FSLTMDCNFI---------------------GFKIPGPSIPVIPLLFMFF 374
                     F+ T+  N I                      F+IP  S+  IP + + F
Sbjct: 338 VKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIF 397

Query: 375 FIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDARTSRFN---- 426
           F+P YE  FVPLARK+TG  +GI  LQRIG GL L   SM  A  VE   R S       
Sbjct: 398 FVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM 457

[Close]

BLAST of Carg25846 vs. ExPASy Swiss-Prot
Match: Q9FM20 (Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-65
Identity = 172/531 (32.39%), Postives = 265/531 (49.91%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA    V E LEN+AF+A A +L+ Y +  M FS + +A  +T FMGT F L L
Sbjct: 56  RHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANAVTAFMGTAFFLAL 115

Query: 75  VGGFVSD--------------------------TECKAANGGQEAILYVGIYLIALGASG 134
           +GGF++D                              +        L+VG+YL+ALG  G
Sbjct: 116 LGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEPWSRVFLFVGLYLVALGVGG 175

Query: 135 VKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFV 194
           +K +LP  GA+QF+E   +   + S FFN+F+FSL+ G+ + +T++VW+    GW + F 
Sbjct: 176 IKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFG 235

Query: 195 VCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVAS-------------------- 254
           V T A+L ++ V  AG   +R  VP GSPI  + +V+ A+                    
Sbjct: 236 VSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTR 295

Query: 255 --------------------------IRNRK---LPLPNNADELHEIRGKEADIPIEI-- 314
                                     +R R+    PL    +++ +++     +PI +  
Sbjct: 296 NDCDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMST 355

Query: 315 ------LEKTDQFSL----TMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARK 374
                 L +   FS+    TM+     F +P  ++PV P++FM    P Y  + +PLARK
Sbjct: 356 IMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARK 415

Query: 375 ITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDAR-----------TSRFN------TT 430
            T   TGI HLQRIG GL+L+  +MA+A  VET  +           +S ++      T 
Sbjct: 416 STKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITF 475

[Close]

BLAST of Carg25846 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 2.1e-63
Identity = 174/533 (32.65%), Postives = 255/533 (47.84%), Query Frame = 0


Query: 15  KRGGNRAAV----TEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           K GG RAA+     +  E M   A+  +L+TY    M+F L+K+A  +TNF+GT F+  L
Sbjct: 41  KHGGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFAL 100

Query: 75  VGGFVSDT------------------------------------------ECKAANGGQE 134
           +GG++SD                                            C+ A G + 
Sbjct: 101 LGGYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKA 160

Query: 135 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 194
            I ++ +YL+ALG+  VK  + A GADQF +  P ++ +LSS+FN   F+ + G  + LT
Sbjct: 161 MIFFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALT 220

Query: 195 LIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNR 254
           L+VW+ T  G D  F V   A+   I+ + +G  +FRN  P+ S    I  VIVA+I  R
Sbjct: 221 LLVWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKR 280

Query: 255 KLPLPNNADELH--------------------------------EIRGKEAD-------- 314
           KL  P++   LH                                +   KE+         
Sbjct: 281 KLASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQ 340

Query: 315 ----------IPI--------EILEKTDQFSLTMDCNF-----IGFKIPGPSIPVIPLLF 374
                     +PI         IL +   FS+    +        F IP  S+  IP + 
Sbjct: 341 VEQVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNSFHIPPASLQAIPYIM 400

Query: 375 MFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDARTS---- 425
           + F +P Y+   VP ARK+TG  +GI  L RIG GL L+  SM  A  +E   R S    
Sbjct: 401 LIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRRDSSVLD 460

[Close]

BLAST of Carg25846 vs. ExPASy TrEMBL
Match: A0A6J1GIY5 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.8e-223
Identity = 432/567 (76.19%), Postives = 437/567 (77.07%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           MCGLGAKNGDKKGPKRGGNRAA    VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT
Sbjct: 1   MCGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           TLTNFMGTTFLLTLVGGFVSDT                                      
Sbjct: 61  TLTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYCLLTAQAHFRELRPEPCKG 120

Query: 121 -----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180
                ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 121 AAAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180

Query: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSP 240
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTAAVLAAILVVCAGKSFFRNNVPKGSP 240

Query: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF------------- 300
           ILRILQVIVASIRNRKLPLPNNADELHEIR KEADIPIEILEKTDQF             
Sbjct: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 300

Query: 301 ---------------------------------------------SLTMDCNFIGFKIPG 360
                                                        SLTMD NFIGFKIPG
Sbjct: 301 RTPWRLCTVTQVEETKILIRMLPIIVSTIFMASCMAQLQTFSIQQSLTMDSNFIGFKIPG 360

Query: 361 PSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420
           PS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 361 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420

Query: 421 T------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLS 448
           T            D+      + +W+   FCVFGMADIFTMVGLLEFFYAESSAEMKSLS
Sbjct: 421 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAEMKSLS 480

[Close]

BLAST of Carg25846 vs. ExPASy TrEMBL
Match: A0A6J1KKU7 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 9.2e-219
Identity = 424/566 (74.91%), Postives = 433/566 (76.50%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           +CGLGAKNGDKKGPKRGGNRAA    VTEGLENMAFVALALSLMTYF+GYMNFSLTKSAT
Sbjct: 16  ICGLGAKNGDKKGPKRGGNRAAGFIYVTEGLENMAFVALALSLMTYFSGYMNFSLTKSAT 75

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           T+TNFMGTTFLLTLVGGFVSDT                                      
Sbjct: 76  TMTNFMGTTFLLTLVGGFVSDTYLSRFKTCVLFACFELLGYFLLSAQAHFRELRPEPCKG 135

Query: 121 -----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 180
                ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN
Sbjct: 136 ALAGSECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFN 195

Query: 181 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSP 240
           WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT AVLAAILVVCAGKSFFRNNVPKGSP
Sbjct: 196 WFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTIAVLAAILVVCAGKSFFRNNVPKGSP 255

Query: 241 ILRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF------------- 300
           ILRILQVIVASI NRKLPLP NADELHEIR KEADIPIEILEKTDQF             
Sbjct: 256 ILRILQVIVASIHNRKLPLPENADELHEIRDKEADIPIEILEKTDQFRFLERAAIIRNDT 315

Query: 301 ---------------------------------------------SLTMDCNFIGFKIPG 360
                                                        SLTMD NF+GFKIPG
Sbjct: 316 RTLWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSLTMDSNFLGFKIPG 375

Query: 361 PSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 420
           PS+PVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE
Sbjct: 376 PSVPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE 435

Query: 421 T------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLS 447
           T            D+      + +W+   FCVFGMADIFTMVGLLEFFYAESSA MKSLS
Sbjct: 436 TRRKNVAIQHNMVDSEEPLPMSVFWLSFQFCVFGMADIFTMVGLLEFFYAESSAGMKSLS 495

[Close]

BLAST of Carg25846 vs. ExPASy TrEMBL
Match: A0A6J1CR11 (protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 672.2 bits (1733), Expect = 1.5e-189
Identity = 370/569 (65.03%), Postives = 404/569 (71.00%), Query Frame = 0


Query: 1   MCGLGAKNGDKKGPKRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSAT 60
           +CGLG +NG+KK  KRGG+RAA    VTEGLENMAFV+LALSL+TYF+GYMNFSLTKSAT
Sbjct: 4   ICGLGNENGEKKEAKRGGSRAAVFVCVTEGLENMAFVSLALSLVTYFSGYMNFSLTKSAT 63

Query: 61  TLTNFMGTTFLLTLVGGFVSDT-------------------------------------- 120
           TLTNFMGTTFLLTL+GGF+SDT                                      
Sbjct: 64  TLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIFASIEFMGYGLLTAQAHFRELRPAPCKG 123

Query: 121 ----ECKAANGGQEAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNW 180
               EC+AA+GGQEAILY+GIYLIALG SGVK ALPALGADQF+ GDPAEAGKLSSFFNW
Sbjct: 124 AVARECEAADGGQEAILYLGIYLIALGTSGVKSALPALGADQFDGGDPAEAGKLSSFFNW 183

Query: 181 FLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPI 240
           FLFSLTTGS VGLTLIVWINT+VGWDWAFVVC+ AV+AAI+ VC+GK F+RNN PKGSPI
Sbjct: 184 FLFSLTTGSIVGLTLIVWINTDVGWDWAFVVCSLAVVAAIVAVCSGKKFYRNNAPKGSPI 243

Query: 241 LRILQVIVASIRNRKLPLPNNADELHEIRGKEADIPIEILEKTDQF-------------- 300
            RI QV VASIRNRKLPLP NADELHEIR KEA +P EILEKTDQF              
Sbjct: 244 QRIFQVFVASIRNRKLPLPKNADELHEIRDKEAAMPYEILEKTDQFRFLDRAAIIRNYTS 303

Query: 301 ----------------------------------------------------SLTMDCNF 360
                                                               S+TMD NF
Sbjct: 304 ASMAAEQQGPWRLCTVTQVEETKILIRMLPIIVSTIFMATCMAQLQTFSIQQSITMDSNF 363

Query: 361 IGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASM 420
           +GFKIPGPS+PVIPLLFM F IPFYERVFVPLARKITGIPTGIRHLQRIG GL+LTAASM
Sbjct: 364 LGFKIPGPSVPVIPLLFMCFLIPFYERVFVPLARKITGIPTGIRHLQRIGIGLVLTAASM 423

Query: 421 AIAGFVET------------DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESS 443
           AIAGFVET            D+      + +W+   FC+FGM D+FT+VGLLEFFYAESS
Sbjct: 424 AIAGFVETRRKNIAIEHDMVDSEEPLPMSVFWLSFQFCIFGMGDMFTLVGLLEFFYAESS 483

[Close]

BLAST of Carg25846 vs. ExPASy TrEMBL
Match: A0A6J1CSN2 (protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111013958 PE=3 SV=1)

HSP 1 Score: 634.8 bits (1636), Expect = 2.7e-178
Identity = 348/536 (64.93%), Postives = 378/536 (70.52%), Query Frame = 0


Query: 30  MAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVGGFVSDT----------- 89
           MAFV+LALSL+TYF+GYMNFSLTKSATTLTNFMGTTFLLTL+GGF+SDT           
Sbjct: 1   MAFVSLALSLVTYFSGYMNFSLTKSATTLTNFMGTTFLLTLLGGFISDTYLSRFKTCLIF 60

Query: 90  -------------------------------ECKAANGGQEAILYVGIYLIALGASGVKV 149
                                          EC+AA+GGQEAILY+GIYLIALG SGVK 
Sbjct: 61  ASIEFMGYGLLTAQAHFRELRPAPCKGAVARECEAADGGQEAILYLGIYLIALGTSGVKS 120

Query: 150 ALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFVVCT 209
           ALPALGADQF+ GDPAEAGKLSSFFNWFLFSLTTGS VGLTLIVWINT+VGWDWAFVVC+
Sbjct: 121 ALPALGADQFDGGDPAEAGKLSSFFNWFLFSLTTGSIVGLTLIVWINTDVGWDWAFVVCS 180

Query: 210 TAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRNRKLPLPNNADELHEIRGKEA 269
            AV+AAI+ VC+GK F+RNN PKGSPI RI QV VASIRNRKLPLP NADELHEIR KEA
Sbjct: 181 LAVVAAIVAVCSGKKFYRNNAPKGSPIQRIFQVFVASIRNRKLPLPKNADELHEIRDKEA 240

Query: 270 DIPIEILEKTDQF----------------------------------------------- 329
            +P EILEKTDQF                                               
Sbjct: 241 AMPYEILEKTDQFRFLDRAAIIRNYTSASMAAEQQGPWRLCTVTQVEETKILIRMLPIIV 300

Query: 330 -------------------SLTMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLA 389
                              S+TMD NF+GFKIPGPS+PVIPLLFM F IPFYERVFVPLA
Sbjct: 301 STIFMATCMAQLQTFSIQQSITMDSNFLGFKIPGPSVPVIPLLFMCFLIPFYERVFVPLA 360

Query: 390 RKITGIPTGIRHLQRIGFGLILTAASMAIAGFVET------------DARTSRFNTTWWI 443
           RKITGIPTGIRHLQRIG GL+LTAASMAIAGFVET            D+      + +W+
Sbjct: 361 RKITGIPTGIRHLQRIGIGLVLTAASMAIAGFVETRRKNIAIEHDMVDSEEPLPMSVFWL 420

[Close]

BLAST of Carg25846 vs. ExPASy TrEMBL
Match: I1K7W4 (Uncharacterized protein OS=Glycine max OX=3847 GN=100781215 PE=3 SV=2)

HSP 1 Score: 562.0 bits (1447), Expect = 2.2e-156
Identity = 304/554 (54.87%), Postives = 359/554 (64.80%), Query Frame = 0


Query: 17  GGNRAAV----TEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTLVG 76
           GGNRAA+     EGLENMAFVA A+SL+TYF GYMNFSLTKSATTLTNF+GT FLL LVG
Sbjct: 18  GGNRAALFVYAMEGLENMAFVANAVSLVTYFFGYMNFSLTKSATTLTNFLGTAFLLALVG 77

Query: 77  GFVSDT---------------------------------------------ECKAANGGQ 136
           G +SDT                                             +C+AA GG 
Sbjct: 78  GLISDTYLSRFKTCVLFACMELLGYGILTVQARFHQLRPIPCKDLAPTQMSQCEAATGGH 137

Query: 137 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 196
            AILY G+YL+ALG  G+K ALPALGADQF+E DP EA +LSSFFNWFLFSLT G+ +G+
Sbjct: 138 AAILYTGLYLVALGTGGIKAALPALGADQFDEKDPKEAAQLSSFFNWFLFSLTIGAIIGV 197

Query: 197 TLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRN 256
           T IVWI   +GWDW+F+VCT  +L AI+ +C G S +RNNVPKGSP++RI+QV VA+ RN
Sbjct: 198 TFIVWIGVNLGWDWSFIVCTLTILFAIVFICMGNSLYRNNVPKGSPLIRIIQVFVAAFRN 257

Query: 257 RKLPLPNNADELHEIRGKEADIPIEILEKTDQF--------------------------- 316
           RKLP+P+N D+LHEI  K      EIL++TDQF                           
Sbjct: 258 RKLPIPDNTDKLHEIHEKRGGDNFEILKRTDQFRFFDRAAIARSSTGAATNSGPWRLCTV 317

Query: 317 ------------------------------------SLTMDCNFIGFKIPGPSIPVIPLL 376
                                               S TM+ N  GFK+PGPS+PVIPL+
Sbjct: 318 TQVEETKILIRMLPIIVSTIFMNTCLAQLQTFTIQQSTTMNTNLGGFKVPGPSVPVIPLM 377

Query: 377 FMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVET-------- 436
           FMF  IP Y+RVFVPLAR+ITGIPTGIRHLQRIG GL+L+A SMA+AGFVET        
Sbjct: 378 FMFVLIPLYDRVFVPLARRITGIPTGIRHLQRIGIGLVLSAVSMAVAGFVETHRKSVAIK 437

Query: 437 ----DARTSRFNTTWWI---FCVFGMADIFTMVGLLEFFYAESSAEMKSLSTAIAWCSIA 444
               D+R     + +W+   + +FG AD+FT++GLLEFFYAESSA MKSL TAI+WCS+A
Sbjct: 438 HNMVDSREPLPISVFWLGFQYAIFGAADMFTLIGLLEFFYAESSAGMKSLGTAISWCSVA 497

[Close]

BLAST of Carg25846 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 276.6 bits (706), Expect = 3.6e-74
Identity = 182/556 (32.73%), Postives = 276/556 (49.64%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA    V E LEN+A++A A +L+ Y   YM+ S +KSA  +TNFMGT FLL L
Sbjct: 25  RHGGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLAL 84

Query: 75  VGGFVSDT---------------------------------------ECKAANGGQEAIL 134
           +GGF+SD                                         C+  +G + A+L
Sbjct: 85  LGGFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMPPSCDSPTCEEVSGSKAAML 144

Query: 135 YVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIV 194
           +VG+YL+ALG  G+K +L + GA+QF+E  P    + S+FFN+F+F L  G+ V +T +V
Sbjct: 145 FVGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVV 204

Query: 195 WINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIV-ASIRNRKL 254
           W+    GW+W F V T A+  +IL+  +G  F+RN +P GSP+  IL+V++ AS++    
Sbjct: 205 WLEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSS 264

Query: 255 PLPNNA-----------------------DELHEIRGKEA-------------------- 314
              +NA                        EL + R +EA                    
Sbjct: 265 GSSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAADE 324

Query: 315 ---------------DIPIEI---------------LEKTDQFSL----TMDCNFIGFKI 374
                          D+ I +               L +   FS+    +M+      KI
Sbjct: 325 KPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAASMNTKIGSLKI 384

Query: 375 PGPSIPVIPLLFMFFFIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGF 430
           P  S+P+ P++F+    P Y+ + +P ARK T   TG+ HLQRIG GL+L+  +MA+A  
Sbjct: 385 PPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMAVAAL 444

[Close]

BLAST of Carg25846 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.7e-74
Identity = 180/535 (33.64%), Postives = 271/535 (50.65%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA      E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 23  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 82

Query: 75  VGGFVSDT------------------------------------------ECKAANGGQE 134
           +GGF++D                                            C+   G + 
Sbjct: 83  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKA 142

Query: 135 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 194
           A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +T
Sbjct: 143 AFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVT 202

Query: 195 LIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVA----- 254
            +VWI    GW+W F V T ++  +ILV   G  F++N +P+GSP+  I +V++A     
Sbjct: 203 FVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS 262

Query: 255 ----------------SIRNRKLP---LPNNADELHE-IRGKEADIPIE----------- 314
                           S    K P   L N+   L++ I GK   I +E           
Sbjct: 263 CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKI 322

Query: 315 ----------------ILEKTDQFSL----TMDCNFIGFKIPGPSIPVIPLLFMFFFIPF 374
                            L +   +S+    TM+   + F +P  S+PV P++FM    P 
Sbjct: 323 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPT 382

Query: 375 YERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE------------TDART 428
           Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE             D+  
Sbjct: 383 YDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEE 442

[Close]

BLAST of Carg25846 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.7e-74
Identity = 180/535 (33.64%), Postives = 271/535 (50.65%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA      E LEN+AF+A A +L+ Y   +M+ SL +S++ +T FM T FLL L
Sbjct: 27  RHGGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLAL 86

Query: 75  VGGFVSDT------------------------------------------ECKAANGGQE 134
           +GGF++D                                            C+   G + 
Sbjct: 87  LGGFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMPPPCKSSAALRCEVVGGSKA 146

Query: 135 AILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLT 194
           A L+VG+YL++LG  G+K +LP+ GA+QF+EG P    + S+FFN+++F L+ G+ V +T
Sbjct: 147 AFLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVT 206

Query: 195 LIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVA----- 254
            +VWI    GW+W F V T ++  +ILV   G  F++N +P+GSP+  I +V++A     
Sbjct: 207 FVVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVS 266

Query: 255 ----------------SIRNRKLP---LPNNADELHE-IRGKEADIPIE----------- 314
                           S    K P   L N+   L++ I GK   I +E           
Sbjct: 267 CSSKTSSNHFTSREVQSEHEEKTPSQSLTNSLTCLNKAIEGKTHHIWLECTVQQVEDVKI 326

Query: 315 ----------------ILEKTDQFSL----TMDCNFIGFKIPGPSIPVIPLLFMFFFIPF 374
                            L +   +S+    TM+   + F +P  S+PV P++FM    P 
Sbjct: 327 VLKMLPIFGCTIMLNCCLAQLSTYSVHQAATMNRKIVNFNVPSASLPVFPVVFMLILAPT 386

Query: 375 YERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVE------------TDART 428
           Y+ + +P ARK+T    GI HLQRIG GL+L+  +MA+A  VE             D+  
Sbjct: 387 YDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVAREAGLLDSEE 446

[Close]

BLAST of Carg25846 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-70
Identity = 186/533 (34.90%), Postives = 263/533 (49.34%), Query Frame = 0


Query: 15  KRGGNRAAV----TEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           K GG RAA+     +  E MA  A+  +L+TY    M+F L+KSA  +TNF+GT FLL+L
Sbjct: 38  KHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFLLSL 97

Query: 75  VGGFVSDT-------------------------------------------ECKAANGGQ 134
           +GGF+SD+                                            C  ANG +
Sbjct: 98  LGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRPPECNMKSTTIHCVEANGYK 157

Query: 135 EAILYVGIYLIALGASGVKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGL 194
            A LY  + L+ALG+  +K  + + GA+QF+  D     KLSSFFN   F+ + G  + L
Sbjct: 158 AATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQLIAL 217

Query: 195 TLIVWINTEVGWDWAFVVCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVASIRN 254
           TL+VW+ T  G D  F V    + A ++ + AG SF+RN  P GS    I QV VA+I  
Sbjct: 218 TLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVAAITK 277

Query: 255 RKLPLPNNADELHE--------------------------IRGKEADIPIEI--LEKTDQ 314
           RK   P+N + +H+                           +GK  + P  +  +E+  Q
Sbjct: 278 RKQICPSNPNMVHQPSTDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRLCTIEQVHQ 337

Query: 315 ----------FSLTMDCNFI---------------------GFKIPGPSIPVIPLLFMFF 374
                     F+ T+  N I                      F+IP  S+  IP + + F
Sbjct: 338 VKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKTFQIPPASLQAIPYIILIF 397

Query: 375 FIPFYERVFVPLARKITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDARTSRFN---- 426
           F+P YE  FVPLARK+TG  +GI  LQRIG GL L   SM  A  VE   R S       
Sbjct: 398 FVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEKKRRESFLEQNVM 457

[Close]

BLAST of Carg25846 vs. TAIR 10
Match: AT5G62730.1 (Major facilitator superfamily protein )

HSP 1 Score: 251.5 bits (641), Expect = 1.2e-66
Identity = 172/531 (32.39%), Postives = 265/531 (49.91%), Query Frame = 0


Query: 15  KRGGNRAA----VTEGLENMAFVALALSLMTYFNGYMNFSLTKSATTLTNFMGTTFLLTL 74
           + GG  AA    V E LEN+AF+A A +L+ Y +  M FS + +A  +T FMGT F L L
Sbjct: 56  RHGGMLAASFVLVVEVLENLAFLANASNLVLYLSTKMGFSPSGAANAVTAFMGTAFFLAL 115

Query: 75  VGGFVSD--------------------------TECKAANGGQEAILYVGIYLIALGASG 134
           +GGF++D                              +        L+VG+YL+ALG  G
Sbjct: 116 LGGFLADAFFTTFHIYLVSAAIEFLGLMVLTVQAHEHSTEPWSRVFLFVGLYLVALGVGG 175

Query: 135 VKVALPALGADQFEEGDPAEAGKLSSFFNWFLFSLTTGSTVGLTLIVWINTEVGWDWAFV 194
           +K +LP  GA+QF+E   +   + S FFN+F+FSL+ G+ + +T++VW+    GW + F 
Sbjct: 176 IKGSLPPHGAEQFDEETSSGRRQRSFFFNYFIFSLSCGALIAVTVVVWLEDNKGWSYGFG 235

Query: 195 VCTTAVLAAILVVCAGKSFFRNNVPKGSPILRILQVIVAS-------------------- 254
           V T A+L ++ V  AG   +R  VP GSPI  + +V+ A+                    
Sbjct: 236 VSTAAILISVPVFLAGSRVYRLKVPSGSPITTLFKVLTAALYAKYKKRRTSRIVVTCHTR 295

Query: 255 --------------------------IRNRK---LPLPNNADELHEIRGKEADIPIEI-- 314
                                     +R R+    PL    +++ +++     +PI +  
Sbjct: 296 NDCDDSVTKQNCDGDDGFLGSFLGEVVRERESLPRPLRCTEEQVKDVKIVIKILPIFMST 355

Query: 315 ------LEKTDQFSL----TMDCNFIGFKIPGPSIPVIPLLFMFFFIPFYERVFVPLARK 374
                 L +   FS+    TM+     F +P  ++PV P++FM    P Y  + +PLARK
Sbjct: 356 IMLNCCLAQLSTFSVQQASTMNTKLGSFTVPPAALPVFPVVFMMILAPTYNHLLLPLARK 415

Query: 375 ITGIPTGIRHLQRIGFGLILTAASMAIAGFVETDAR-----------TSRFN------TT 430
            T   TGI HLQRIG GL+L+  +MA+A  VET  +           +S ++      T 
Sbjct: 416 STKTETGITHLQRIGTGLVLSIVAMAVAALVETKRKHVVVSCCSNNNSSSYSSSPLPITF 475

The following BLAST results are available for this feature:

BLAST of Carg25846 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020549.1	1.8e-253	100.00	Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. argyrosperm...	[more]
XP_022951878.1	5.7e-223	76.19	protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata]	[more]
KAG6585639.1	1.3e-222	76.74	Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023002887.1	1.9e-218	74.91	protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima]	[more]
XP_023537870.1	8.0e-217	74.43	protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg25846 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8H157	5.1e-73	32.73	Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1	[more]
Q8VYE4	6.7e-73	33.64	Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1	[more]
Q56XQ6	1.5e-69	34.90	Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1	[more]
Q9FM20	1.8e-65	32.39	Protein NRT1/ PTR FAMILY 4.7 OS=Arabidopsis thaliana OX=3702 GN=NPF4.7 PE=2 SV=2	[more]
Q93VV5	2.1e-63	32.65	Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1	[more]

BLAST of Carg25846 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GIY5	2.8e-223	76.19	protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111454615 ...	[more]
A0A6J1KKU7	9.2e-219	74.91	protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111496623 PE...	[more]
A0A6J1CR11	1.5e-189	65.03	protein NRT1/ PTR FAMILY 4.5-like isoform X1 OS=Momordica charantia OX=3673 GN=L...	[more]
A0A6J1CSN2	2.7e-178	64.93	protein NRT1/ PTR FAMILY 4.5-like isoform X2 OS=Momordica charantia OX=3673 GN=L...	[more]
I1K7W4	2.2e-156	54.87	Uncharacterized protein OS=Glycine max OX=3847 GN=100781215 PE=3 SV=2	[more]

BLAST of Carg25846 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G69850.1	3.6e-74	32.73	nitrate transporter 1:2	[more]
AT1G27040.2	4.7e-74	33.64	Major facilitator superfamily protein	[more]
AT1G27040.1	4.7e-74	33.64	Major facilitator superfamily protein	[more]
AT1G33440.1	1.1e-70	34.90	Major facilitator superfamily protein	[more]
AT5G62730.1	1.2e-66	32.39	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 237..434 e-value: 2.7E-53 score: 182.9 coord: 5..236 e-value: 1.2E-54 score: 187.3
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 89..431
IPR000109	Proton-dependent oligopeptide transporter family	PFAM	PF00854	PTR2	coord: 240..386 e-value: 7.4E-25 score: 87.6 coord: 77..209 e-value: 1.1E-32 score: 113.4
IPR000109	Proton-dependent oligopeptide transporter family	PANTHER	PTHR11654	OLIGOPEPTIDE TRANSPORTER-RELATED	coord: 239..434 coord: 79..240 coord: 11..78
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..20
None	No IPR available	PANTHER	PTHR11654:SF110	PEPTIDE/NITRATE TRANSPORTER	coord: 239..434 coord: 79..240 coord: 11..78

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25846-RA	Carg25846-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity