Carg25829 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25829
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	GDSL esterase/lipase
Location	Carg_Chr05: 5868924 .. 5870444 (+)
RNA-Seq Expression	Carg25829
Synteny	Carg25829

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGTTTTTCTTCTTCTTCTCTTATTGTGAAGGAGGAAGAGAAATAAGAAAGGGCAATGGAGGGATGATAAAGGGAATGTTTGTGTTTGGGAGCTCTCTAGTTGACAATGGCAACAATAACTTTTTAGAGAACTCAAGGGCTAAAGCGGACTTTTTACCTTACGGAATGGATTTTTCTCTCGGTCCGTTGGGACGATTTACGAACGGCAAGAATGTTATCGACCTTCTCGGAGATTATTTTCACCTTCCTTCTCCCATTCCTCCGTTCACTGATCCTTCAACAAAGGGGAAGAAGATTGTTCATGGCGTCAACTATGCTTCTGGTGGTTCTGGTATTCTAGATAACACCGGTGCCATCACGGTAAGATACAAAAACTCACACTTAGCAATGAAAACACGTAAACCAATCTTGTTTTGTTTGTGTTGCGTAGGGAAATGTAATAAGTTTGAACAGACAAATCAAAAACTTTGAAACCGTGACGTTGCCTGAATTGAGAAGAGGTATGAGCACAAGGGTATCGTTAGACGAGTATTTGTTTGTGGTTGGATCGGGAGGGAACGATTACTCATTCAACTACTTTTTGAGTAACCTCCACTCGGAACTCAGCCTCAACTCGTTCACAGCCAACCTCACAGCCACATTGTCTCAACAACTCAAAGTGGGTTGGTTTCGTTTATGATTCAAGCCACAAATTTGAACCACATTCTTTGTTTATGTCAATTAATATTGTGTTGCTTTGATCCACAGAAGCTGTATGGTTTGGGAGCAAGGAAAATAGTGTTGATATCAGTGAACCCATTAGGGTGCAGTCCAATGGTGAGAGCAAATAACAAAGGACAATGCCTTGATATACTAAACCAAGCTGCTCAAATGTTCAATCTCAACTTGAAGACATTGGTGGATGACCTAAAACCACAAATGCCACTCTCCAATCTCGTCTTCCTTAATTCGTACAAGATTATCGATGATATTATCTCTCACCATATTTCTAAAGGTACCCCACATATTTATCTTTTCTACTTTACTTCTGTCCTTTTCGCTCTACTAGCTTACGTTCAAGTTCGGATTTGGTTGTTAAGCTCATTAATAGCTTATAAACATGGCTAAAATTTATTTAGAGGGTCAATCATGAAACCGTTCTACTTATAGTATTACTAACCATAAGAAAGTTAGCCATAAGATCTAATAATGATTTTATTATTATTATGAACTTGATATTTATTTTCAAATATCAAATAATTAATTGTGTTTGGTAAAAGAGAAACAAATAGTGTGGGACAACGATCATTGATAGCATTGATAGGGCGTACCTAAATTTTATTATTTGAAGGATACGGTTCCCTAATATTTAAGTCCATTTACGATCGGTAGGACACTTCATTAATCTTTAAAGTTCATATCATAAATGTGGCATTTAGGGTAATCTCTATTTTACTTGTTCTTGCACTCATTTTATTCTCAGATTTAAGCATTAAAAGGGATTTACCACCATTAAACACTTTTTACCAAAAGAAGAAATTT

mRNA sequence

TTGTTTTTCTTCTTCTTCTCTTATTGTGAAGGAGGAAGAGAAATAAGAAAGGGCAATGGAGGGATGATAAAGGGAATGTTTGTGTTTGGGAGCTCTCTAGTTGACAATGGCAACAATAACTTTTTAGAGAACTCAAGGGCTAAAGCGGACTTTTTACCTTACGGAATGGATTTTTCTCTCGGTCCGTTGGGACGATTTACGAACGGCAAGAATGTTATCGACCTTCTCGGAGATTATTTTCACCTTCCTTCTCCCATTCCTCCGTTCACTGATCCTTCAACAAAGGGGAAGAAGATTGTTCATGGCGTCAACTATGCTTCTGGTGGTTCTGGTATTCTAGATAACACCGGTGCCATCACGGGAAATGTAATAAGTTTGAACAGACAAATCAAAAACTTTGAAACCGTGACGTTGCCTGAATTGAGAAGAGGTATGAGCACAAGGGTATCGTTAGACGAGTATTTGTTTGTGGTTGGATCGGGAGGGAACGATTACTCATTCAACTACTTTTTGAGTAACCTCCACTCGGAACTCAGCCTCAACTCGTTCACAGCCAACCTCACAGCCACATTGTCTCAACAACTCAAAAAGCTGTATGGTTTGGGAGCAAGGAAAATAGTGTTGATATCAGTGAACCCATTAGGGTGCAGTCCAATGGTGAGAGCAAATAACAAAGGACAATGCCTTGATATACTAAACCAAGCTGCTCAAATGTTCAATCTCAACTTGAAGACATTGGTGGATGACCTAAAACCACAAATGCCACTCTCCAATCTCGTCTTCCTTAATTCGTACAAGATTATCGATGATATTATCTCTCACCATATTTCTAAAGGTACCCCACATATTTATCTTTTCTACTTTACTTCTGTCCTTTTCGCTCTACTAGCTTACGTTCAAGTTCGGATTTGGTTGTTAAGCTCATTAATAGCTTATAAACATGGCTAAAATTTATTTAGAGGGTCAATCATGAAACCGTTCTACTTATAGTATTACTAACCATAAGAAAGTTAGCCATAAGATCTAATAATGATTTTATTATTATTATGAACTTGATATTTATTTTCAAATATCAAATAATTAATTGTGTTTGGTAAAAGAGAAACAAATAGTGTGGGACAACGATCATTGATAGCATTGATAGGGCGTACCTAAATTTTATTATTTGAAGGATACGGTTCCCTAATATTTAAGTCCATTTACGATCGGTAGGACACTTCATTAATCTTTAAAGTTCATATCATAAATGTGGCATTTAGGGTAATCTCTATTTTACTTGTTCTTGCACTCATTTTATTCTCAGATTTAAGCATTAAAAGGGATTTACCACCATTAAACACTTTTTACCAAAAGAAGAAATTT

Coding sequence (CDS)

ATGATAAAGGGAATGTTTGTGTTTGGGAGCTCTCTAGTTGACAATGGCAACAATAACTTTTTAGAGAACTCAAGGGCTAAAGCGGACTTTTTACCTTACGGAATGGATTTTTCTCTCGGTCCGTTGGGACGATTTACGAACGGCAAGAATGTTATCGACCTTCTCGGAGATTATTTTCACCTTCCTTCTCCCATTCCTCCGTTCACTGATCCTTCAACAAAGGGGAAGAAGATTGTTCATGGCGTCAACTATGCTTCTGGTGGTTCTGGTATTCTAGATAACACCGGTGCCATCACGGGAAATGTAATAAGTTTGAACAGACAAATCAAAAACTTTGAAACCGTGACGTTGCCTGAATTGAGAAGAGGTATGAGCACAAGGGTATCGTTAGACGAGTATTTGTTTGTGGTTGGATCGGGAGGGAACGATTACTCATTCAACTACTTTTTGAGTAACCTCCACTCGGAACTCAGCCTCAACTCGTTCACAGCCAACCTCACAGCCACATTGTCTCAACAACTCAAAAAGCTGTATGGTTTGGGAGCAAGGAAAATAGTGTTGATATCAGTGAACCCATTAGGGTGCAGTCCAATGGTGAGAGCAAATAACAAAGGACAATGCCTTGATATACTAAACCAAGCTGCTCAAATGTTCAATCTCAACTTGAAGACATTGGTGGATGACCTAAAACCACAAATGCCACTCTCCAATCTCGTCTTCCTTAATTCGTACAAGATTATCGATGATATTATCTCTCACCATATTTCTAAAGGTACCCCACATATTTATCTTTTCTACTTTACTTCTGTCCTTTTCGCTCTACTAGCTTACGTTCAAGTTCGGATTTGGTTGTTAAGCTCATTAATAGCTTATAAACATGGCTAA

Protein sequence

MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELRRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGLGARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVFLNSYKIIDDIISHHISKGTPHIYLFYFTSVLFALLAYVQVRIWLLSSLIAYKHG

Homology

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BLAST of Carg25829 vs. NCBI nr
Match: KAG7029938.1 (GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 584.7 bits (1506), Expect = 4.4e-163
Identity = 294/294 (100.00%), Postives = 294/294 (100.00%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL
Sbjct: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL
Sbjct: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240

Query: 241 LNSYKIIDDIISHHISKGTPHIYLFYFTSVLFALLAYVQVRIWLLSSLIAYKHG 295
           LNSYKIIDDIISHHISKGTPHIYLFYFTSVLFALLAYVQVRIWLLSSLIAYKHG
Sbjct: 241 LNSYKIIDDIISHHISKGTPHIYLFYFTSVLFALLAYVQVRIWLLSSLIAYKHG 294

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BLAST of Carg25829 vs. NCBI nr
Match: XP_022932048.1 (GDSL esterase/lipase At4g16230-like [Cucurbita moschata])

HSP 1 Score: 513.1 bits (1320), Expect = 1.6e-141
Identity = 256/258 (99.22%), Postives = 257/258 (99.61%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 33  MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 92

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL
Sbjct: 93  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 152

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRGM TRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSL+SFTANLTATLSQQLKKLYGL
Sbjct: 153 RRGMRTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLDSFTANLTATLSQQLKKLYGL 212

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 213 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 272

Query: 241 LNSYKIIDDIISHHISKG 259
           LNSYKIIDDIISHHISKG
Sbjct: 273 LNSYKIIDDIISHHISKG 290

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BLAST of Carg25829 vs. NCBI nr
Match: XP_023520891.1 (GDSL esterase/lipase At4g16230-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 510.0 bits (1312), Expect = 1.4e-140
Identity = 254/258 (98.45%), Postives = 257/258 (99.61%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 39  MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 98

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQI+NFETVTLPEL
Sbjct: 99  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIRNFETVTLPEL 158

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSL+SFTANLTATLSQQLKK+YGL
Sbjct: 159 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLDSFTANLTATLSQQLKKVYGL 218

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCL ILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 219 GARKIVLISVNPLGCSPMVRANNKGQCLHILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 278

Query: 241 LNSYKIIDDIISHHISKG 259
           LNSYKIIDDIISHHISKG
Sbjct: 279 LNSYKIIDDIISHHISKG 296

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BLAST of Carg25829 vs. NCBI nr
Match: XP_022972885.1 (GDSL esterase/lipase At4g16230-like [Cucurbita maxima])

HSP 1 Score: 503.1 bits (1294), Expect = 1.7e-138
Identity = 251/258 (97.29%), Postives = 253/258 (98.06%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 38  MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 97

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPF DPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLN QIKNFETVT+PEL
Sbjct: 98  LPSPIPPFADPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNTQIKNFETVTMPEL 157

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRG S RV LDEYLFVVGSGGNDYSFNYFLSNLHSELSL+SFTANLTATLSQQLKKLYGL
Sbjct: 158 RRGRSMRVLLDEYLFVVGSGGNDYSFNYFLSNLHSELSLDSFTANLTATLSQQLKKLYGL 217

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 218 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 277

Query: 241 LNSYKIIDDIISHHISKG 259
           LNSYKIIDDIISHHISKG
Sbjct: 278 LNSYKIIDDIISHHISKG 295

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BLAST of Carg25829 vs. NCBI nr
Match: KAG6607604.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 445.7 bits (1145), Expect = 3.2e-121
Identity = 223/224 (99.55%), Postives = 223/224 (99.55%), Query Frame = 0


Query: 35  MDFSLGPLGRFTNGKNVIDLLGDYFHLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDN 94
           MDFSLGPLGRFTNGKNVIDLLGDYFHLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDN
Sbjct: 1   MDFSLGPLGRFTNGKNVIDLLGDYFHLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDN 60

Query: 95  TGAITGNVISLNRQIKNFETVTLPELRRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLH 154
           TGAITGNVISLNRQIKNFETVTLPELRRGMSTRV LDEYLFVVGSGGNDYSFNYFLSNLH
Sbjct: 61  TGAITGNVISLNRQIKNFETVTLPELRRGMSTRVLLDEYLFVVGSGGNDYSFNYFLSNLH 120

Query: 155 SELSLNSFTANLTATLSQQLKKLYGLGARKIVLISVNPLGCSPMVRANNKGQCLDILNQA 214
           SELSLNSFTANLTATLSQQLKKLYGLGARKIVLISVNPLGCSPMVRANNKGQCLDILNQA
Sbjct: 121 SELSLNSFTANLTATLSQQLKKLYGLGARKIVLISVNPLGCSPMVRANNKGQCLDILNQA 180

Query: 215 AQMFNLNLKTLVDDLKPQMPLSNLVFLNSYKIIDDIISHHISKG 259
           AQMFNLNLKTLVDDLKPQMPLSNLVFLNSYKIIDDIISHHISKG
Sbjct: 181 AQMFNLNLKTLVDDLKPQMPLSNLVFLNSYKIIDDIISHHISKG 224

[Close]

BLAST of Carg25829 vs. ExPASy Swiss-Prot
Match: Q9FVV1 (GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 1.9e-47
Identity = 118/266 (44.36%), Postives = 155/266 (58.27%), Query Frame = 0


Query: 2   IKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHL 61
           +  MFV G SLVD GNNNFL+ + A+A+FLPYG+D +  P GRF+NG   IDLL     +
Sbjct: 39  VPAMFVLGDSLVDAGNNNFLQ-TVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEI 98

Query: 62  PSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR 121
           PSP PPF DP+T G +I+ GVNYAS  +GILD +G   G   SLN+Q+ N ET TL +LR
Sbjct: 99  PSP-PPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLET-TLSQLR 158

Query: 122 RGMSTRVSLD---EYLFVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLK 181
             MS +   D     L V+  G NDY  NY + NL+          F   L +  ++QL 
Sbjct: 159 TMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLL 218

Query: 182 KLYGLGARKIVLISVNPLGCSPMVRANN---KGQCLDILNQAAQMFNLNLKTLVDDLKPQ 241
            LY LG RKI +  V PLGC P  RA       +C+D +NQ    FN  LK+LVD L  +
Sbjct: 219 TLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQR 278

Query: 242 MPLSNLVFLNSYKIIDDIISHHISKG 259
            P +  V+ N+Y  I DI+++  + G
Sbjct: 279 SPGAIYVYGNTYSAIGDILNNPAAYG 301

[Close]

BLAST of Carg25829 vs. ExPASy Swiss-Prot
Match: Q9FNP2 (GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 1.9e-44
Identity = 115/272 (42.28%), Postives = 156/272 (57.35%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLG-PLGRFTNGKNVIDLLGDYF 60
           M   MFVFG SLVDNGNNN L NS A++++LPYG+DF+   P GRF+NGK ++D +G+  
Sbjct: 46  MFPAMFVFGDSLVDNGNNNHL-NSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGELL 105

Query: 61  HLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPE 120
            LP  IP F D    G  I+HGVNYAS   GIL+ TG   G   S+ RQ++NFE  TL E
Sbjct: 106 GLPE-IPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEK-TLME 165

Query: 121 LRRGMSTRVSLDEY----LFVVGSGGNDYSFNYFLSNLHSELSL---NSFTANLTATLSQ 180
           + R M  + S+ EY    L VV  G NDY  NY    L    S+    SF   L +  + 
Sbjct: 166 ISRSM-RKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTT 225

Query: 181 QLKKLYGLGARKIVLISVNPLGCSP---MVRANNKGQCLDILNQAAQMFNLNLKTLVDDL 240
            L +LYG G RK V+  V PLGC P     +A   G+C++ +N+ A++FN  L +LVD L
Sbjct: 226 HLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRL 285

Query: 241 KPQMPLSN---LVFLNSYKIIDDIISHHISKG 259
                 ++    V+ N+Y    DI+++  + G
Sbjct: 286 NSDNKTASEAIFVYGNTYGAAVDILTNPFNYG 313

[Close]

BLAST of Carg25829 vs. ExPASy Swiss-Prot
Match: O23470 (GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3 SV=2)

HSP 1 Score: 178.7 bits (452), Expect = 9.5e-44
Identity = 108/266 (40.60%), Postives = 159/266 (59.77%), Query Frame = 0


Query: 2   IKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHL 61
           I   FVFG SLVD GNNN+L  + +KA+++P G+DF   P GRFTNG+ ++D++      
Sbjct: 28  IPANFVFGDSLVDAGNNNYLA-TLSKANYVPNGIDFG-SPTGRFTNGRTIVDIVYQALGS 87

Query: 62  PSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR 121
               PP+  P+T G  I++GVNYASGGSGIL++TG + G  I+++ Q+ NF T     + 
Sbjct: 88  DELTPPYLAPTTSGSLILNGVNYASGGSGILNSTGKLFGERINVDAQLDNFATTRQDIIS 147

Query: 122 -RGMSTRVSL-DEYLFVVGSGGNDYSFNYF---LSNLHSE-LSLNSFTANLTATLSQQLK 181
             G S    L    +F V +G ND   NYF   +S L  + ++   F   + +    QL 
Sbjct: 148 WIGESEAAKLFRSAIFSVTTGSNDLINNYFTPVISTLQRKVVAPEVFVDTMISKFRLQLT 207

Query: 182 KLYGLGARKIVLISVNPLGCSPMVRANNK---GQCLDILNQAAQMFNLNLKTLVDDLKPQ 241
           +LY LGARKIV+I++ P+GC P  R ++      CL   N+ AQM+NL LKTLV++L   
Sbjct: 208 RLYQLGARKIVVINIGPIGCIPFERESDPAAGNNCLAEPNEVAQMYNLKLKTLVEELNKN 267

Query: 242 MPLSNLVFLNSYKIIDDIISHHISKG 259
           +  S  V+ + ++I+DDII ++ S G
Sbjct: 268 LQGSRFVYGDVFRIVDDIIQNYSSYG 291

[Close]

BLAST of Carg25829 vs. ExPASy Swiss-Prot
Match: Q8LFJ9 (GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 4.4e-41
Identity = 103/257 (40.08%), Postives = 137/257 (53.31%), Query Frame = 0


Query: 6   FVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHLPSPI 65
           FVFG SLVD+GNNN++  + A+A++ PYG+DF   P GRF NG+ V+D    Y  LP  +
Sbjct: 32  FVFGDSLVDSGNNNYIP-TLARANYFPYGIDFGF-PTGRFCNGRTVVDYGATYLGLPL-V 91

Query: 66  PPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELRRGMS 125
           PP+  P + G+  + GVNYAS  +GILD TG   G   + N QI  FE      LRR   
Sbjct: 92  PPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQ 151

Query: 126 TRVSLDEYL----FVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLKKLY 185
               L +YL      +  G NDY  NY +   +S     S   +   L  TLS Q+ +LY
Sbjct: 152 NPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLY 211

Query: 186 GLGARKIVLISVNPLGCSP----MVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMP 245
            LGARK+VL    PLGC P    MV  NN   C+  +N    MFN  LK L + L   +P
Sbjct: 212 NLGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTLNTTLP 271

Query: 246 LSNLVFLNSYKIIDDII 252
            S  V+ N + +  D++
Sbjct: 272 GSFFVYQNVFDLFHDMV 285

[Close]

BLAST of Carg25829 vs. ExPASy Swiss-Prot
Match: Q9C7N4 (GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.3e-40
Identity = 98/256 (38.28%), Postives = 148/256 (57.81%), Query Frame = 0


Query: 6   FVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHLPSPI 65
           FVFG SLVDNGNNN L  S A++++ PYG+DF  GP GRF+NGK  +D++ +       I
Sbjct: 34  FVFGDSLVDNGNNNGL-ISIARSNYFPYGIDFG-GPTGRFSNGKTTVDVIAELLGFNGYI 93

Query: 66  PPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR-RGM 125
           P +   +  G++I+ GVNYAS  +GI + TG   G  IS + Q++N++T     ++  G 
Sbjct: 94  PAYN--TVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQVVQLLGD 153

Query: 126 STRVS--LDEYLFVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLKKLYG 185
            TR +  L   ++ VG G NDY  NYF+   +S   + +   +  +L +  S QL  LY 
Sbjct: 154 ETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYSTQLNALYN 213

Query: 186 LGARKIVLISVNPLGCSPMVRANNKG--QCLDILNQAAQMFNLNLKTLVDDLKPQMPLSN 245
            GARK  L  +  +GCSP   A +     C+D +N A Q+FN  L++LVD L    P + 
Sbjct: 214 YGARKFALSGIGAVGCSPNALAGSPDGRTCVDRINSANQIFNNKLRSLVDQLNNNHPDAK 273

Query: 246 LVFLNSYKIIDDIISH 254
            +++N+Y I  D+I++
Sbjct: 274 FIYINAYGIFQDMITN 285

[Close]

BLAST of Carg25829 vs. ExPASy TrEMBL
Match: A0A6J1F0J0 (GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata OX=3662 GN=LOC111438381 PE=3 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 7.9e-142
Identity = 256/258 (99.22%), Postives = 257/258 (99.61%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 33  MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 92

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL
Sbjct: 93  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 152

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRGM TRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSL+SFTANLTATLSQQLKKLYGL
Sbjct: 153 RRGMRTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLDSFTANLTATLSQQLKKLYGL 212

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 213 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 272

Query: 241 LNSYKIIDDIISHHISKG 259
           LNSYKIIDDIISHHISKG
Sbjct: 273 LNSYKIIDDIISHHISKG 290

[Close]

BLAST of Carg25829 vs. ExPASy TrEMBL
Match: A0A6J1I9W6 (GDSL esterase/lipase At4g16230-like OS=Cucurbita maxima OX=3661 GN=LOC111471396 PE=3 SV=1)

HSP 1 Score: 503.1 bits (1294), Expect = 8.1e-139
Identity = 251/258 (97.29%), Postives = 253/258 (98.06%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH
Sbjct: 38  MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 97

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPSPIPPF DPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLN QIKNFETVT+PEL
Sbjct: 98  LPSPIPPFADPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNTQIKNFETVTMPEL 157

Query: 121 RRGMSTRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 180
           RRG S RV LDEYLFVVGSGGNDYSFNYFLSNLHSELSL+SFTANLTATLSQQLKKLYGL
Sbjct: 158 RRGRSMRVLLDEYLFVVGSGGNDYSFNYFLSNLHSELSLDSFTANLTATLSQQLKKLYGL 217

Query: 181 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 240
           GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF
Sbjct: 218 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 277

Query: 241 LNSYKIIDDIISHHISKG 259
           LNSYKIIDDIISHHISKG
Sbjct: 278 LNSYKIIDDIISHHISKG 295

[Close]

BLAST of Carg25829 vs. ExPASy TrEMBL
Match: A0A1S3CG14 (GDSL esterase/lipase At4g16230-like OS=Cucumis melo OX=3656 GN=LOC103500044 PE=3 SV=1)

HSP 1 Score: 405.6 bits (1041), Expect = 1.8e-109
Identity = 205/264 (77.65%), Postives = 226/264 (85.61%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           MIKGMFVFGSSLVDNGNNNFLE SRAKAD+LPYG+DF +GP GRFTNGKN+IDLLG Y  
Sbjct: 48  MIKGMFVFGSSLVDNGNNNFLEKSRAKADYLPYGIDFGVGPSGRFTNGKNIIDLLGRYLG 107

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LPS IPPF DPSTKG  IV GVNYASGGSGILD+TG+I GNV SLN+QIKNFE VTLPEL
Sbjct: 108 LPSSIPPFFDPSTKGTNIVRGVNYASGGSGILDDTGSIAGNVTSLNKQIKNFEEVTLPEL 167

Query: 121 RRGMSTRVS------LDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQL 180
           RR M  R        L+ YLFVVGSGGNDYSFNYFL+N   +L+L  FTANLTATLS QL
Sbjct: 168 RRLMGRRNGRRKGSLLENYLFVVGSGGNDYSFNYFLTNSDPQLTLPIFTANLTATLSTQL 227

Query: 181 KKLYGLGARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMP 240
           KKLY LGARK+V ISVNPLGCSPMVRANNKG+C++ILNQAAQ+FNLNLKTLVDD+KPQ+P
Sbjct: 228 KKLYSLGARKMVAISVNPLGCSPMVRANNKGECIEILNQAAQLFNLNLKTLVDDIKPQIP 287

Query: 241 LSNLVFLNSYKIIDDIISHHISKG 259
           LSN+VFLNSY II+DIIS   S+G
Sbjct: 288 LSNIVFLNSYNIINDIISQPASQG 311

[Close]

BLAST of Carg25829 vs. ExPASy TrEMBL
Match: A0A6J1II88 (GDSL esterase/lipase At5g08460-like OS=Cucurbita maxima OX=3661 GN=LOC111477152 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.9e-109
Identity = 207/258 (80.23%), Postives = 224/258 (86.82%), Query Frame = 0


Query: 2   IKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHL 61
           IKGMFVFGSSLVDNGNNN LENSRAKA++LPYGMDF LGP GRFTNGKNVID LGDY  L
Sbjct: 27  IKGMFVFGSSLVDNGNNNALENSRAKANYLPYGMDFPLGPSGRFTNGKNVIDFLGDYLGL 86

Query: 62  PSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR 121
           PS IP F D  TKG KIVHGVNYASGGSGILDNTG ++GNV +LNRQIKNFE VTLPELR
Sbjct: 87  PSSIPTFFDTLTKGMKIVHGVNYASGGSGILDNTGIVSGNVTTLNRQIKNFEEVTLPELR 146

Query: 122 RGMS-TRVSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 181
             M   + SLD YLFVVGSGGNDYSFNYFL+N  +++SL  FTANLTATLS QLKKLY L
Sbjct: 147 GLMGRKKSSLDNYLFVVGSGGNDYSFNYFLTNSDAQVSLPVFTANLTATLSHQLKKLYSL 206

Query: 182 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 241
           GARK+V+ISVNPLGCSPMVRANNKGQC++ LNQAAQ+FNLNLKTLVD LKPQMP SNLVF
Sbjct: 207 GARKMVVISVNPLGCSPMVRANNKGQCIETLNQAAQLFNLNLKTLVDVLKPQMPHSNLVF 266

Query: 242 LNSYKIIDDIISHHISKG 259
           LNSY+II+DIIS   SKG
Sbjct: 267 LNSYQIINDIISQPASKG 284

[Close]

BLAST of Carg25829 vs. ExPASy TrEMBL
Match: A0A6J1EXA8 (GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata OX=3662 GN=LOC111439041 PE=3 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 3.9e-109
Identity = 208/258 (80.62%), Postives = 225/258 (87.21%), Query Frame = 0


Query: 2   IKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHL 61
           IKGMFVFGSSLVDNGNNN LENSRAKA++LPYGMDF LGP GRFTNGKNVID LGDY  L
Sbjct: 32  IKGMFVFGSSLVDNGNNNVLENSRAKANYLPYGMDFPLGPSGRFTNGKNVIDFLGDYLGL 91

Query: 62  PSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR 121
           PS IP F+DPSTKG KIVHGVNYASGGSGILDNTG I+GNV +LNRQIKNFE  TLPELR
Sbjct: 92  PSSIPTFSDPSTKGMKIVHGVNYASGGSGILDNTGIISGNVTTLNRQIKNFEEGTLPELR 151

Query: 122 RGMSTR-VSLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNSFTANLTATLSQQLKKLYGL 181
             +  R  SLD YLFVVGSGGNDYSFNYFL+N  +++SL  FTANLTATLS QLKKLY L
Sbjct: 152 GLIGRRKSSLDNYLFVVGSGGNDYSFNYFLTNSDAQVSLPVFTANLTATLSGQLKKLYSL 211

Query: 182 GARKIVLISVNPLGCSPMVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMPLSNLVF 241
           GARK+V+ISVNPLGCSPMVRANNKGQC++ LNQAAQ+FNLNLKTLVD LK QMP SNLVF
Sbjct: 212 GARKMVVISVNPLGCSPMVRANNKGQCIETLNQAAQLFNLNLKTLVDVLKLQMPRSNLVF 271

Query: 242 LNSYKIIDDIISHHISKG 259
           LNSY+II+DIIS   SKG
Sbjct: 272 LNSYQIINDIISQPASKG 289

[Close]

BLAST of Carg25829 vs. TAIR 10
Match: AT1G71250.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 191.0 bits (484), Expect = 1.3e-48
Identity = 118/266 (44.36%), Postives = 155/266 (58.27%), Query Frame = 0


Query: 2   IKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHL 61
           +  MFV G SLVD GNNNFL+ + A+A+FLPYG+D +  P GRF+NG   IDLL     +
Sbjct: 39  VPAMFVLGDSLVDAGNNNFLQ-TVARANFLPYGIDMNYQPTGRFSNGLTFIDLLARLLEI 98

Query: 62  PSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR 121
           PSP PPF DP+T G +I+ GVNYAS  +GILD +G   G   SLN+Q+ N ET TL +LR
Sbjct: 99  PSP-PPFADPTTSGNRILQGVNYASAAAGILDVSGYNYGGRFSLNQQMVNLET-TLSQLR 158

Query: 122 RGMSTRVSLD---EYLFVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLK 181
             MS +   D     L V+  G NDY  NY + NL+          F   L +  ++QL 
Sbjct: 159 TMMSPQNFTDYLARSLVVLVFGSNDYINNYLMPNLYDSSIRFRPPDFANLLLSQYARQLL 218

Query: 182 KLYGLGARKIVLISVNPLGCSPMVRANN---KGQCLDILNQAAQMFNLNLKTLVDDLKPQ 241
            LY LG RKI +  V PLGC P  RA       +C+D +NQ    FN  LK+LVD L  +
Sbjct: 219 TLYSLGLRKIFIPGVAPLGCIPNQRARGISPPDRCVDSVNQILGTFNQGLKSLVDQLNQR 278

Query: 242 MPLSNLVFLNSYKIIDDIISHHISKG 259
            P +  V+ N+Y  I DI+++  + G
Sbjct: 279 SPGAIYVYGNTYSAIGDILNNPAAYG 301

[Close]

BLAST of Carg25829 vs. TAIR 10
Match: AT5G08460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 181.0 bits (458), Expect = 1.4e-45
Identity = 115/272 (42.28%), Postives = 156/272 (57.35%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLG-PLGRFTNGKNVIDLLGDYF 60
           M   MFVFG SLVDNGNNN L NS A++++LPYG+DF+   P GRF+NGK ++D +G+  
Sbjct: 46  MFPAMFVFGDSLVDNGNNNHL-NSLARSNYLPYGIDFAGNQPTGRFSNGKTIVDFIGELL 105

Query: 61  HLPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPE 120
            LP  IP F D    G  I+HGVNYAS   GIL+ TG   G   S+ RQ++NFE  TL E
Sbjct: 106 GLPE-IPAFMDTVDGGVDILHGVNYASAAGGILEETGRHLGERFSMGRQVENFEK-TLME 165

Query: 121 LRRGMSTRVSLDEY----LFVVGSGGNDYSFNYFLSNLHSELSL---NSFTANLTATLSQ 180
           + R M  + S+ EY    L VV  G NDY  NY    L    S+    SF   L +  + 
Sbjct: 166 ISRSM-RKESVKEYMAKSLVVVSLGNNDYINNYLKPRLFLSSSIYDPTSFADLLLSNFTT 225

Query: 181 QLKKLYGLGARKIVLISVNPLGCSP---MVRANNKGQCLDILNQAAQMFNLNLKTLVDDL 240
            L +LYG G RK V+  V PLGC P     +A   G+C++ +N+ A++FN  L +LVD L
Sbjct: 226 HLLELYGKGFRKFVIAGVGPLGCIPDQLAAQAALPGECVEAVNEMAELFNNRLVSLVDRL 285

Query: 241 KPQMPLSN---LVFLNSYKIIDDIISHHISKG 259
                 ++    V+ N+Y    DI+++  + G
Sbjct: 286 NSDNKTASEAIFVYGNTYGAAVDILTNPFNYG 313

[Close]

BLAST of Carg25829 vs. TAIR 10
Match: AT5G15720.1 (GDSL-motif lipase 7 )

HSP 1 Score: 169.9 bits (429), Expect = 3.1e-42
Identity = 103/257 (40.08%), Postives = 137/257 (53.31%), Query Frame = 0


Query: 6   FVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHLPSPI 65
           FVFG SLVD+GNNN++  + A+A++ PYG+DF   P GRF NG+ V+D    Y  LP  +
Sbjct: 32  FVFGDSLVDSGNNNYIP-TLARANYFPYGIDFGF-PTGRFCNGRTVVDYGATYLGLPL-V 91

Query: 66  PPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELRRGMS 125
           PP+  P + G+  + GVNYAS  +GILD TG   G   + N QI  FE      LRR   
Sbjct: 92  PPYLSPLSIGQNALRGVNYASAAAGILDETGRHYGARTTFNGQISQFEITIELRLRRFFQ 151

Query: 126 TRVSLDEYL----FVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLKKLY 185
               L +YL      +  G NDY  NY +   +S     S   +   L  TLS Q+ +LY
Sbjct: 152 NPADLRKYLAKSIIGINIGSNDYINNYLMPERYSTSQTYSGEDYADLLIKTLSAQISRLY 211

Query: 186 GLGARKIVLISVNPLGCSP----MVRANNKGQCLDILNQAAQMFNLNLKTLVDDLKPQMP 245
            LGARK+VL    PLGC P    MV  NN   C+  +N    MFN  LK L + L   +P
Sbjct: 212 NLGARKMVLAGSGPLGCIPSQLSMVTGNNTSGCVTKINNMVSMFNSRLKDLANTLNTTLP 271

Query: 246 LSNLVFLNSYKIIDDII 252
            S  V+ N + +  D++
Sbjct: 272 GSFFVYQNVFDLFHDMV 285

[Close]

BLAST of Carg25829 vs. TAIR 10
Match: AT1G29670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 9.1e-42
Identity = 98/256 (38.28%), Postives = 148/256 (57.81%), Query Frame = 0


Query: 6   FVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFHLPSPI 65
           FVFG SLVDNGNNN L  S A++++ PYG+DF  GP GRF+NGK  +D++ +       I
Sbjct: 34  FVFGDSLVDNGNNNGL-ISIARSNYFPYGIDFG-GPTGRFSNGKTTVDVIAELLGFNGYI 93

Query: 66  PPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPELR-RGM 125
           P +   +  G++I+ GVNYAS  +GI + TG   G  IS + Q++N++T     ++  G 
Sbjct: 94  PAYN--TVSGRQILSGVNYASAAAGIREETGRQLGQRISFSGQVRNYQTTVSQVVQLLGD 153

Query: 126 STRVS--LDEYLFVVGSGGNDYSFNYFLSNLHS---ELSLNSFTANLTATLSQQLKKLYG 185
            TR +  L   ++ VG G NDY  NYF+   +S   + +   +  +L +  S QL  LY 
Sbjct: 154 ETRAADYLKRCIYSVGLGSNDYLNNYFMPTFYSSSRQFTPEQYANDLISRYSTQLNALYN 213

Query: 186 LGARKIVLISVNPLGCSPMVRANNKG--QCLDILNQAAQMFNLNLKTLVDDLKPQMPLSN 245
            GARK  L  +  +GCSP   A +     C+D +N A Q+FN  L++LVD L    P + 
Sbjct: 214 YGARKFALSGIGAVGCSPNALAGSPDGRTCVDRINSANQIFNNKLRSLVDQLNNNHPDAK 273

Query: 246 LVFLNSYKIIDDIISH 254
            +++N+Y I  D+I++
Sbjct: 274 FIYINAYGIFQDMITN 285

[Close]

BLAST of Carg25829 vs. TAIR 10
Match: AT1G71691.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 9.1e-42
Identity = 99/265 (37.36%), Postives = 155/265 (58.49%), Query Frame = 0


Query: 1   MIKGMFVFGSSLVDNGNNNFLENSRAKADFLPYGMDFSLGPLGRFTNGKNVIDLLGDYFH 60
           ++  +FVFG SL+DNGNNN +  S AKA++ PYG+DF+ GP GRF NG  ++D +     
Sbjct: 52  IVPALFVFGDSLIDNGNNNNIP-SFAKANYFPYGIDFNGGPTGRFCNGLTMVDGIAQLLG 111

Query: 61  LPSPIPPFTDPSTKGKKIVHGVNYASGGSGILDNTGAITGNVISLNRQIKNFETVTLPEL 120
           LP  IP +++ +  G +++ GVNYAS  +GIL +TG      I  ++QI NFET TL ++
Sbjct: 112 LPL-IPAYSEAT--GDQVLRGVNYASAAAGILPDTGGNFVGRIPFDQQIHNFET-TLDQV 171

Query: 121 --RRGMSTRV--SLDEYLFVVGSGGNDYSFNYFLSNLHSELSLNS--FTANLTATLSQQL 180
             + G +  +  S+   LF +G G NDY  NY + N  +    NS  F   L    + QL
Sbjct: 172 ASKSGGAVAIADSVTRSLFFIGMGSNDYLNNYLMPNFPTRNQYNSQQFGDLLVQHYTDQL 231

Query: 181 KKLYGLGARKIVLISVNPLGCSPMVRA-NNKGQCLDILNQAAQMFNLNLKTLVDDLKPQM 240
            +LY LG RK V+  +  +GC P + A  N G+C + +NQ    FN N+KT++ +L   +
Sbjct: 232 TRLYNLGGRKFVVAGLGRMGCIPSILAQGNDGKCSEEVNQLVLPFNTNVKTMISNLNQNL 291

Query: 241 PLSNLVFLNSYKIIDDIISHHISKG 259
           P +  ++L+   + +DI+++  + G
Sbjct: 292 PDAKFIYLDIAHMFEDIVANQAAYG 311

The following BLAST results are available for this feature:

BLAST of Carg25829 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029938.1	4.4e-163	100.00	GDSL esterase/lipase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022932048.1	1.6e-141	99.22	GDSL esterase/lipase At4g16230-like [Cucurbita moschata]	[more]
XP_023520891.1	1.4e-140	98.45	GDSL esterase/lipase At4g16230-like [Cucurbita pepo subsp. pepo]	[more]
XP_022972885.1	1.7e-138	97.29	GDSL esterase/lipase At4g16230-like [Cucurbita maxima]	[more]
KAG6607604.1	3.2e-121	99.55	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg25829 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FVV1	1.9e-47	44.36	GDSL esterase/lipase At1g71250 OS=Arabidopsis thaliana OX=3702 GN=At1g71250 PE=2...	[more]
Q9FNP2	1.9e-44	42.28	GDSL esterase/lipase At5g08460 OS=Arabidopsis thaliana OX=3702 GN=At5g08460 PE=2...	[more]
O23470	9.5e-44	40.60	GDSL esterase/lipase At4g16230 OS=Arabidopsis thaliana OX=3702 GN=At4g16230 PE=3...	[more]
Q8LFJ9	4.4e-41	40.08	GDSL esterase/lipase 7 OS=Arabidopsis thaliana OX=3702 GN=GLIP7 PE=2 SV=1	[more]
Q9C7N4	1.3e-40	38.28	GDSL esterase/lipase At1g29670 OS=Arabidopsis thaliana OX=3702 GN=At1g29670 PE=2...	[more]

BLAST of Carg25829 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F0J0	7.9e-142	99.22	GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata OX=3662 GN=LOC11143838...	[more]
A0A6J1I9W6	8.1e-139	97.29	GDSL esterase/lipase At4g16230-like OS=Cucurbita maxima OX=3661 GN=LOC111471396 ...	[more]
A0A1S3CG14	1.8e-109	77.65	GDSL esterase/lipase At4g16230-like OS=Cucumis melo OX=3656 GN=LOC103500044 PE=3...	[more]
A0A6J1II88	3.9e-109	80.23	GDSL esterase/lipase At5g08460-like OS=Cucurbita maxima OX=3661 GN=LOC111477152 ...	[more]
A0A6J1EXA8	3.9e-109	80.62	GDSL esterase/lipase At4g16230-like OS=Cucurbita moschata OX=3662 GN=LOC11143904...	[more]

BLAST of Carg25829 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G71250.1	1.3e-48	44.36	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G08460.1	1.4e-45	42.28	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT5G15720.1	3.1e-42	40.08	GDSL-motif lipase 7	[more]
AT1G29670.1	9.1e-42	38.28	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G71691.2	9.1e-42	37.36	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 1..265 e-value: 6.8E-54 score: 185.4
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 6..249 e-value: 1.0E-19 score: 71.9
None	No IPR available	PANTHER	PTHR45650	GDSL-LIKE LIPASE/ACYLHYDROLASE-RELATED	coord: 1..258
None	No IPR available	PANTHER	PTHR45650:SF2	OS06G0560700 PROTEIN	coord: 1..258

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25829-RA	Carg25829-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds