Carg25828 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25828
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionProtein of unknown function (DUF803)
LocationCarg_Chr05: 5870498 .. 5874270 (-)
RNA-Seq ExpressionCarg25828
SyntenyCarg25828
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TGTCCACTCTGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCGTCTGCAAATTTTTGGAATTAGATGAATCTGTGCAGCAATCCATGAGCACCCATGACCCTTTCATCTCGCCCAATGATAACCTGAAAGGTTTCCTTCTTGCTATGTTATCCAGCGCCTTTATTGGCTCCAGCTTCATCATCAAGAAGTTGGGTCTTCGTCGGGCCGGGGCTTCCGGCTCTCGAGCGAGTCAGTTTTTCTTCAATTATTTTTTTCATCTGCCTGGGTTCTTCCAATTTCGTCTTCCATTTTACCTGTATGCTTGGTTTGATTTCGTTGATTTACATGTTCGAGGTGCTCGTTCTTCTTGCGATTCTGTCATTCTCATGATTGAATTTTTCAGTTCGCGAAGAATTAATTTGTTTCATTTACATTGTGAATGGTTACAATTGAGGGTTGTAGATTGTGGTTTCTCCTTTTTGTTACTAATTGGCTGGCTGCGCTGCTCACATTGTTCCTTTGGGTTTATGCTGATGAAATTTGTGTGGTTAATGAATTCTAGGTTCTGGTGGATATGGATATCTGTTAGAGCCACTTTGGTGGATCGGCATGAGTACTAGTGAGTATCAATTTCAACTGTCTTGGGACTCTTTACTCTCCCTGCTTATCTCTAAGGAATGCTCATAACTCAGAGTTACATTTAACTAAATCTCAAACCTCATTCTAGATCTATTAGATTCTTTAAAAATCCTTTTGTTCCTCTCCACTCGAATTCCGAGAGCGTCTCCAAGCATTTCAAGTCTTTTTAGCGCGTTTTCGAGTTTTTACATGCAGTTTATAAGCTACATTTGATTGATTACTTTCACCTGTGTACCATTTGCAGTGATTGTTGGAGAATTTTCTAATTTTGTGGCTTATGTTTATGCTCCTGCTATTCTGGTAACACCACTTGGAGCGATCAGTATAATTGTTAGGTAACAAGCTTCAATGAGATATTGATTTGGGATTGAATGTAATTACTTTTTATTTATGCTTCCATGTCAAAATGGGATTTACCCAAATGATTTTCATGCGTTTATTGTGTTGGCAGTGCTGTGCTTGCACATTTTTTCTTGAAGGAAAAATTGGAGAAAATGGATGTATTGGGGTGTGTATTATGTGTTGTAGGGTCTACAATGATTGTTCTTCATGCGCCTGGTGAACGTACTCCGAGTTCAGTGGACGAGATATGGGAACTAGCTACTCAACCAAGTAAAGTAAACTCTAAAACATATCCTGATGTGTGTCTTACTGCGTGGAGCTTCTTTTTGAATATTCTGATGTGTTTTGTTCAGCTTTCCTTCTCTATACGGCCTCGGTTATAGCTATTGTGTTGTTCCTCGTGTTGTACTGCGAACCCCGTTACGGACAGACAAATATACTCATTTACATTGGGATATGCTCCATAATTGGATCCTTGACGGTCTGTCTTCGTTACCCTTTAACGTGCTGAATCGTTCTGATGATTCATGGTTGATTTTTCTTTTTCTGTGAAACAGTTTTAATCTTTCTGCATTGGTTTGCAGGTAATGAGCATCAAGGCTATTGGCATTGCTATAAAACTCACAATGGAGGGTTTGAGTCAGGTTGCTCACTTCCAAACATGGGTCTTTGTAATGGTTGCAATCTCATGCATAATTGTTCAGCTGAATTATTTAAACAAGGTTAGTGTACTACTGTTTTGTTCAAATGGAGTTCGTGGATGTAGAACTTTTTTGCTCGGTAATCGTTTGGTGTAGAGTACATTTGTGGCTTCTTCATATCTCCCCTGTGACGTATTTATAGTTTCGTTATGGCTATTATTATCTTTCGTGTGTTTTACGTATGGAGAATCCAAACCTCTCGAATTTTGAGTTATATGGAACTTTCACTTGAATGTTGATGAATAATTATGTCGGAGTATCAGTTGGTACGAGAACTCTATTTTTGTCATGCTAAATATTAAGAAGATGAATACTCGTTTAGATGTAATTATGTCGAGTATCGATCCAGTTGTGTATCAATTGGCACCAGAGCTCTATTGTTGCCATGCGAAATATTAAGAAGATGAATACTCGTTTAGATATAGGCCATGGAACGGAATTGTAGAATATGCTCAGCTGCTGTTCAAGTGTGAGAGAGGGATTGTGAGAGAGAATATGGTCATCCCTTAAGGCTATTTTAAAACTTAAGCCGGAAAAGATCTTTACATGAACAGGCCTTGGACACATTTGACACCGCAGTTGTCTCACCAATTCATTATGCAATGTTCACATCCTTCACGATCTTCGCCAGTGCCATAATGTTCAAGGTACTTTTGCTACAAATTTAAACATTTCTTTTGGGCTTTTCAAAGAGATTGATTCATGTGCTTGTTATACCATTAGGACTGGTCTGGTCAGAGTGCTAGCAGCATTGCATCAGAACTTTGTGGATTTGTAACCATACTTTCTGGGACTATGGTCTTGCACGCTACAAGATCACCCGATCCCGCCTCTGTTTCAGGTATTTATTTCATTCGAAACTCTTAAAACTGGCCGAATGCAGAGTTTGTGATGATTCATTGTCTGTTATTTATGACATCTTTTGCTAGATTATTAATTGTTCTTTATACAATCACATGCTACTAGTATCTATGATCAACATATATATTGTACACGAGTAGACACTTGTTAAGCACAATGGATATGTTCTTAATACTTGTTAAGCTTGAATATGAACGAGACGATTATTTGGGCGTCGAACACGTGTTAAACACAATAGACAATGCCTACTTGTTACAGAGATGTACCTGCCTGTGTCTCCTCAAGTGTCATGGTATTTCCAAGCAAATGGTGATACCTGGAAACGGAAATCTGAAGAAATACCATTGCCAGATTTTGATGCAATCCTCAAACAAGACCATTTCACATGAGATGAAAGGTATTATTGGCTCTCTCCATTGCACACAAAACAAATGCATCCAAATTCTGGGAGAAATCTGCGTATACTTGCTTTTCTCACATTGTTTTAATGAGTTATTCGGTAGAGTGGTACACCAAAGTTCCATTTTCTTTCTTGCTCGACCGCTTGAGATTGAAGAACGGGTAGGGCGTGTGTAACTTAGTATGTTCTTTGTAACACTTTACTTTGTTCCTAAATAGGTAACTGGTATTGGAAACACAGCTTCTTCCCCTCTCTTCTTACTTTTAGGCCTTACACCTCTGATAACATTATTTTGTACAGTAGATCAGAGCCTGTGGATGGAAGATGTAATCTGCTTTTGTGTGTAGTTTTTCACTCATTTCTCTTGTCCAAGGAAAAATGTGAGATTCGATAGACGAATGTATCAATATTGGACTTTGAAATGACACGATGAAAGCTTGAAAGGTGAATAAAGTTCTAATGTTATTACTACTACACACTGACAACTTACAGAGAAAGAGCGAGATTCCCAAATTGAGCGTAACTCAATTAGTGAAGACATACTTTTAATTAAGAGGTCAGAGGTTCAAATCTCTCTACTCTACTCTACTCTACTCTACATGTCAAGAATCTATAGATTGACGAGGTGAAAAAGATTAGTGGGATAAACTTCGGTTTGAAGTTGAGAAGCATAAGCCTTAGATGCTATGAGGACATTGACAGCTTCGGTTGGATGTAACCCGTCGAAGAAGACATGGTTGGTTCTATTTGGACAAGTTTGGCCTCCATTTTTGCACAAAACTCCATTCCCACCTTCACTCGGAGATGGCACTTCACAGCAGGGCACAGCTGCCTCCATAAACCCTG

mRNA sequence

TGTCCACTCTGCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCGTCTGCAAATTTTTGGAATTAGATGAATCTGTGCAGCAATCCATGAGCACCCATGACCCTTTCATCTCGCCCAATGATAACCTGAAAGGTTTCCTTCTTGCTATGTTATCCAGCGCCTTTATTGGCTCCAGCTTCATCATCAAGAAGTTGGGTCTTCGTCGGGCCGGGGCTTCCGGCTCTCGAGCGAGTTCTGGTGGATATGGATATCTGTTAGAGCCACTTTGGTGGATCGGCATGAGTACTATGATTGTTGGAGAATTTTCTAATTTTGTGGCTTATGTTTATGCTCCTGCTATTCTGGTAACACCACTTGGAGCGATCAGTATAATTGTTAGTGCTGTGCTTGCACATTTTTTCTTGAAGGAAAAATTGGAGAAAATGGATGTATTGGGGTGTGTATTATGTGTTGTAGGGTCTACAATGATTGTTCTTCATGCGCCTGGTGAACGTACTCCGAGTTCAGTGGACGAGATATGGGAACTAGCTACTCAACCAACTTTCCTTCTCTATACGGCCTCGGTTATAGCTATTGTGTTGTTCCTCGTGTTGTACTGCGAACCCCGTTACGGACAGACAAATATACTCATTTACATTGGGATATGCTCCATAATTGGATCCTTGACGGTAATGAGCATCAAGGCTATTGGCATTGCTATAAAACTCACAATGGAGGGTTTGAGTCAGGTTGCTCACTTCCAAACATGGGTCTTTGTAATGGTTGCAATCTCATGCATAATTGTTCAGCTGAATTATTTAAACAAGAGTACATTTGTGGCTTCTTCATATCTCCCCTGTGACGTATTTATAGTTTCGTTATGGCTATTATTATCTTTCGTGTGTTTTACCCGGAAAAGATCTTTACATGAACAGGCCTTGGACACATTTGACACCGCAGTTGTCTCACCAATTCATTATGCAATGTTCACATCCTTCACGATCTTCGCCAGTGCCATAATGTTCAAGGACTGGTCTGGTCAGAGTGCTAGCAGCATTGCATCAGAACTTTGTGGATTTGTAACCATACTTTCTGGGACTATGGTCTTGCACGCTACAAGATCACCCGATCCCGCCTCTGTTTCAGAGATGTACCTGCCTGTGTCTCCTCAAGTGTCATGGTATTTCCAAGCAAATGGTGATACCTGGAAACGGAAATCTGAAGAAATACCATTGCCAGATTTTGATGCAATCCTCAAACAAGACCATTTCACATGAGATGAAAGGTATTATTGGCTCTCTCCATTGCACACAAAACAAATGCATCCAAATTCTGGGAGAAATCTGCGTATACTTGCTTTTCTCACATTGTTTTAATGAGTTATTCGGTAGAGTGGTACACCAAAGTTCCATTTTCTTTCTTGCTCGACCGCTTGAGATTGAAGAACGGGTAGGGCGTGTGTAACTTAGTATGTTCTTTGTAACACTTTACTTTGTTCCTAAATAGGTAACTGGTATTGGAAACACAGCTTCTTCCCCTCTCTTCTTACTTTTAGGCCTTACACCTCTGATAACATTATTTTGTACAGTAGATCAGAGCCTGTGGATGGAAGATGTAATCTGCTTTTGTGTGTAGTTTTTCACTCATTTCTCTTGTCCAAGGAAAAATGTGAGATTCGATAGACGAATGTATCAATATTGGACTTTGAAATGACACGATGAAAGCTTGAAAGGTGAATAAAGTTCTAATGTTATTACTACTACACACTGACAACTTACAGAGAAAGAGCGAGATTCCCAAATTGAGCGTAACTCAATTAGTGAAGACATACTTTTAATTAAGAGGTCAGAGGTTCAAATCTCTCTACTCTACTCTACTCTACTCTACATGTCAAGAATCTATAGATTGACGAGGTGAAAAAGATTAGTGGGATAAACTTCGGTTTGAAGTTGAGAAGCATAAGCCTTAGATGCTATGAGGACATTGACAGCTTCGGTTGGATGTAACCCGTCGAAGAAGACATGGTTGGTTCTATTTGGACAAGTTTGGCCTCCATTTTTGCACAAAACTCCATTCCCACCTTCACTCGGAGATGGCACTTCACAGCAGGGCACAGCTGCCTCCATAAACCCTG

Coding sequence (CDS)

ATGAGCACCCATGACCCTTTCATCTCGCCCAATGATAACCTGAAAGGTTTCCTTCTTGCTATGTTATCCAGCGCCTTTATTGGCTCCAGCTTCATCATCAAGAAGTTGGGTCTTCGTCGGGCCGGGGCTTCCGGCTCTCGAGCGAGTTCTGGTGGATATGGATATCTGTTAGAGCCACTTTGGTGGATCGGCATGAGTACTATGATTGTTGGAGAATTTTCTAATTTTGTGGCTTATGTTTATGCTCCTGCTATTCTGGTAACACCACTTGGAGCGATCAGTATAATTGTTAGTGCTGTGCTTGCACATTTTTTCTTGAAGGAAAAATTGGAGAAAATGGATGTATTGGGGTGTGTATTATGTGTTGTAGGGTCTACAATGATTGTTCTTCATGCGCCTGGTGAACGTACTCCGAGTTCAGTGGACGAGATATGGGAACTAGCTACTCAACCAACTTTCCTTCTCTATACGGCCTCGGTTATAGCTATTGTGTTGTTCCTCGTGTTGTACTGCGAACCCCGTTACGGACAGACAAATATACTCATTTACATTGGGATATGCTCCATAATTGGATCCTTGACGGTAATGAGCATCAAGGCTATTGGCATTGCTATAAAACTCACAATGGAGGGTTTGAGTCAGGTTGCTCACTTCCAAACATGGGTCTTTGTAATGGTTGCAATCTCATGCATAATTGTTCAGCTGAATTATTTAAACAAGAGTACATTTGTGGCTTCTTCATATCTCCCCTGTGACGTATTTATAGTTTCGTTATGGCTATTATTATCTTTCGTGTGTTTTACCCGGAAAAGATCTTTACATGAACAGGCCTTGGACACATTTGACACCGCAGTTGTCTCACCAATTCATTATGCAATGTTCACATCCTTCACGATCTTCGCCAGTGCCATAATGTTCAAGGACTGGTCTGGTCAGAGTGCTAGCAGCATTGCATCAGAACTTTGTGGATTTGTAACCATACTTTCTGGGACTATGGTCTTGCACGCTACAAGATCACCCGATCCCGCCTCTGTTTCAGAGATGTACCTGCCTGTGTCTCCTCAAGTGTCATGGTATTTCCAAGCAAATGGTGATACCTGGAAACGGAAATCTGAAGAAATACCATTGCCAGATTTTGATGCAATCCTCAAACAAGACCATTTCACATGA

Protein sequence

MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYFQANGDTWKRKSEEIPLPDFDAILKQDHFT
Homology
BLAST of Carg25828 vs. NCBI nr
Match: KAG7029939.1 (putative magnesium transporter NIPA6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 756.9 bits (1953), Expect = 8.5e-215
Identity = 389/389 (100.00%), Postives = 389/389 (100.00%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL
Sbjct: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK
Sbjct: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
           STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF
Sbjct: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           QANGDTWKRKSEEIPLPDFDAILKQDHFT
Sbjct: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 389

BLAST of Carg25828 vs. NCBI nr
Match: KAG6607605.1 (putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 667.5 bits (1721), Expect = 6.8e-188
Identity = 353/389 (90.75%), Postives = 353/389 (90.75%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL
Sbjct: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK
Sbjct: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF
Sbjct: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           QANGDTWKRKSEEIPLPDFDAILKQDHFT
Sbjct: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 353

BLAST of Carg25828 vs. NCBI nr
Match: XP_023520888.1 (probable magnesium transporter NIPA6 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 664.5 bits (1713), Expect = 5.8e-187
Identity = 352/389 (90.49%), Postives = 352/389 (90.49%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 67  MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 126

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL
Sbjct: 127 WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 186

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 187 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 246

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK
Sbjct: 247 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 306

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 307 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 366

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF
Sbjct: 367 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 419

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           QANGDTWKRKSEEI LPDFDAILKQDHFT
Sbjct: 427 QANGDTWKRKSEEITLPDFDAILKQDHFT 419

BLAST of Carg25828 vs. NCBI nr
Match: XP_022932049.1 (probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata])

HSP 1 Score: 662.9 bits (1709), Expect = 1.7e-186
Identity = 351/389 (90.23%), Postives = 351/389 (90.23%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           MS HDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MSPHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL
Sbjct: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK
Sbjct: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF
Sbjct: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           QANGDTWKRKSEEIP PDFDAILKQDHFT
Sbjct: 361 QANGDTWKRKSEEIPSPDFDAILKQDHFT 353

BLAST of Carg25828 vs. NCBI nr
Match: XP_038897340.1 (probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida] >XP_038897341.1 probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida])

HSP 1 Score: 642.1 bits (1655), Expect = 3.1e-180
Identity = 340/390 (87.18%), Postives = 349/390 (89.49%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           M+TH+P ISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MTTHEPSISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGM TMIVGEFSNFVAY+YAPAILVTPLGAISIIVSAVLAHFFLKEKL+KM VLGC+L
Sbjct: 61  WWIGMITMIVGEFSNFVAYIYAPAILVTPLGAISIIVSAVLAHFFLKEKLQKMGVLGCIL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEG SQVAHFQTWVFVMVAISCII+QLNYLNK
Sbjct: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGWSQVAHFQTWVFVMVAISCIIIQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQ-ALDTFDTAVVSPIHYAMFTSFTI 300
                          +S W L            H   ALDTFDTAVVSPIHYAMFTSFTI
Sbjct: 241 VK-------------ISFWSLYF--------GFHSNLALDTFDTAVVSPIHYAMFTSFTI 300

Query: 301 FASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWY 360
           FAS IMFKDWSGQSASSIASELCGFVTILSGT+VLH TRSPDPASVSEMY+ VSPQVSWY
Sbjct: 301 FASVIMFKDWSGQSASSIASELCGFVTILSGTVVLHDTRSPDPASVSEMYMSVSPQVSWY 360

Query: 361 FQANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           F ANGDTWKRKSEEI LPDFDAILKQDHFT
Sbjct: 361 FPANGDTWKRKSEEILLPDFDAILKQDHFT 369

BLAST of Carg25828 vs. ExPASy Swiss-Prot
Match: Q8GWX2 (Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g21120 PE=2 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.6e-113
Identity = 219/362 (60.50%), Postives = 267/362 (73.76%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN KG +LA+ SS FIGSSFI+KK GL+RAGA G+RA  GGY YLLEPLWW GM TMIVG
Sbjct: 4   DNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIVG 63

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NFVAY+YAPA+LVTPLGA+SII+SAVLAHF LKEKL+KM VLGCV C+VGS +IV+H
Sbjct: 64  EAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVIH 123

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+TP+SV+EIW LATQP FL+Y A  ++IVL L+L+ EP  GQTNIL+YIGICS++G
Sbjct: 124 APKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLMG 183

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           +LTVMSIKAIGIAIKLTMEG+SQ+ + QTW+FVMVA++C++ QL YLNK           
Sbjct: 184 ALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNK----------- 243

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALDTF+ A+VSP++Y MFT+ TI ASAIMFKDWSG
Sbjct: 244 -------------------------ALDTFNAAIVSPVYYVMFTTLTIVASAIMFKDWSG 303

Query: 312 QSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYFQANGDTWKRKS 371
           Q A+S+ASELCGF+T+L+GTM+LH TR  +    S      S  V WY     D+ K  +
Sbjct: 304 QDAASVASELCGFITVLTGTMILHGTREEEQQQAS------SEHVRWY-----DSRKSMN 318

Query: 372 EE 374
           EE
Sbjct: 364 EE 318

BLAST of Carg25828 vs. ExPASy Swiss-Prot
Match: Q8GYS1 (Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g38730 PE=2 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 2.1e-107
Identity = 202/338 (59.76%), Postives = 252/338 (74.56%), Query Frame = 0

Query: 11  NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIV 70
           +DN  G +LA+ SS FIGSSFI+KK GL+RA A+G+RA  GGY YLLEPLWW+G+ TM  
Sbjct: 3   SDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTMTF 62

Query: 71  GEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVL 130
           GE +NFVAYVYAPA+LVTPLGA+SII+SAVLAHF L EKL KM V GCV C+VGS MIV+
Sbjct: 63  GEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMIVI 122

Query: 131 HAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSII 190
           HAP E+TP+SV+EIW+LA QP FL+Y A  ++IVL L+LYCEP  GQTNIL+YIGICS++
Sbjct: 123 HAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICSLM 182

Query: 191 GSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLP 250
           GSLTVMSIKA+GIAIKLT EG++Q+ + +TW F MVA  C+++Q+ YLNK          
Sbjct: 183 GSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNK---------- 242

Query: 251 CDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWS 310
                                     ALDTF+ A+VSPI+Y MFT+ TI ASAIMFKDW+
Sbjct: 243 --------------------------ALDTFNAAIVSPIYYVMFTTLTIVASAIMFKDWN 302

Query: 311 GQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEM 349
           GQ+  SIASE+CGF+T+L+GT++LH+TR  + AS   M
Sbjct: 303 GQNTDSIASEICGFITVLTGTVILHSTREEEQASPRRM 304

BLAST of Carg25828 vs. ExPASy Swiss-Prot
Match: Q9LIR9 (Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g23870 PE=2 SV=1)

HSP 1 Score: 370.5 bits (950), Expect = 2.3e-101
Identity = 190/327 (58.10%), Postives = 237/327 (72.48%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN+ G +LA+ SS FIGSSFIIKK GL++AGASG RA  GGYGYL EP WW GM TMIVG
Sbjct: 7   DNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITMIVG 66

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NF AY +APAILVTPLGA+SII SAVLAHF LKEKL    +LGC+LCVVGST IVLH
Sbjct: 67  EVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCILCVVGSTTIVLH 126

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+   SV +IW+LA +P FL+Y+A ++ +V  L+ Y EPRYG+T++++Y+GICS++G
Sbjct: 127 APHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVYVGICSLMG 186

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           SLTVMS+KA+ IAIKLT  G +Q  +F TW+F++V  +C I+Q+NYLNK           
Sbjct: 187 SLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNK----------- 246

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALDTF+TAV+SP++Y MFT+FTI AS IMFKDW+ 
Sbjct: 247 -------------------------ALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWAS 297

Query: 312 QSASSIASELCGFVTILSGTMVLHATR 339
           QS   IA+ELCGFVTILSGT +LH T+
Sbjct: 307 QSGLKIATELCGFVTILSGTFLLHKTK 297

BLAST of Carg25828 vs. ExPASy Swiss-Prot
Match: Q9LNK7 (Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g34470 PE=2 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.5e-100
Identity = 200/383 (52.22%), Postives = 255/383 (66.58%), Query Frame = 0

Query: 11  NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIV 70
           +DN+KG +LA+ SS FIG+SFI+KK GL+RAGASG RA SGGY YLLEPLWW+GM TMIV
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 71  GEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVL 130
           GE +NF AY +APAILVTPLGA+SII+SA LAH  L EKL    +LGCVLCVVGS  IVL
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 131 HAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSII 190
           HAP E+   SV ++W LAT+P FLLY A+V+   + L++   P+YGQ+++++YIG+CS++
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 191 GSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLP 250
           GSL+VMS+KA+GIA+KLT  G++Q+ + QTWVF ++ ++C+I Q+NYLNK          
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNK---------- 256

Query: 251 CDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWS 310
                                     ALDTF+TAVVSPI+Y MFTS TI AS IMFKDW 
Sbjct: 257 --------------------------ALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWD 316

Query: 311 GQSASSIASELCGFVTILSGTMVLHATRSPDPASVS----EMYLPVSPQVSWYFQANGDT 370
            Q  + I +ELCGFVTILSGT +LH T+     S S     + LP   + S  F+  G  
Sbjct: 317 RQDGTQIVTELCGFVTILSGTFLLHKTKDMVDGSSSLGNLALRLPKQLEDSNGFEQEGIP 363

Query: 371 WKRKSEEI---PLPDFDAILKQD 387
              +  E    P P    IL QD
Sbjct: 377 LTLRRHECTKSPRPMRQFILPQD 363

BLAST of Carg25828 vs. ExPASy Swiss-Prot
Match: B3LFA3 (Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g13800 PE=2 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 3.3e-100
Identity = 189/327 (57.80%), Postives = 235/327 (71.87%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN+ G +LA+ SS FIGSSFIIKK GL++AG SG+RA  GGYGYL EP WW GM TMIVG
Sbjct: 7   DNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITMIVG 66

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NF AY +APAILVTPLGA+SII SAVLAHF L+EKL    +LGCVLCVVGST IVLH
Sbjct: 67  EIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGILGCVLCVVGSTTIVLH 126

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+   SV ++W LAT+P FL Y+A V+ +VL L+ Y EPRYG+T++++Y+GICS++G
Sbjct: 127 APHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVYVGICSLMG 186

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           SLTVMS+KA+ IAIKLT  G++Q  +F  W+F++V   C I+Q+NYLNK           
Sbjct: 187 SLTVMSVKAVAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNK----------- 246

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALD F+TAV+SP++Y MFT+FTI AS IMFKDW+ 
Sbjct: 247 -------------------------ALDNFNTAVISPVYYVMFTTFTILASMIMFKDWAS 297

Query: 312 QSASSIASELCGFVTILSGTMVLHATR 339
           QS   IA+ELCGFVTILSGT +LH T+
Sbjct: 307 QSGLQIATELCGFVTILSGTFLLHKTK 297

BLAST of Carg25828 vs. ExPASy TrEMBL
Match: A0A6J1EVY4 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111438382 PE=3 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 8.1e-187
Identity = 351/389 (90.23%), Postives = 351/389 (90.23%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           MS HDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MSPHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL
Sbjct: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK
Sbjct: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF
Sbjct: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
           QANGDTWKRKSEEIP PDFDAILKQDHFT
Sbjct: 361 QANGDTWKRKSEEIPSPDFDAILKQDHFT 353

BLAST of Carg25828 vs. ExPASy TrEMBL
Match: A0A0A0K4P7 (Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_7G291140 PE=3 SV=1)

HSP 1 Score: 629.4 bits (1622), Expect = 9.9e-177
Identity = 331/389 (85.09%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           M+TH+P ISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MTTHEPSISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGM TMIVGEFSNFVAY+YAPAILVTPLGAISIIVSAVLAHFFLKEKL+KM VLGC+L
Sbjct: 61  WWIGMITMIVGEFSNFVAYIYAPAILVTPLGAISIIVSAVLAHFFLKEKLQKMGVLGCIL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELA QPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELAIQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIY+GICSIIGSLTVMSIKAIGIAIKLTMEG SQVAHFQTWVF+MVAISCII+QLNYLNK
Sbjct: 181 LIYVGICSIIGSLTVMSIKAIGIAIKLTMEGWSQVAHFQTWVFLMVAISCIIIQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           AS IMFKDWSGQSASSIASELCGF+TILSGT+VLH TRS DPASVSEMY+ VSPQVSWYF
Sbjct: 301 ASVIMFKDWSGQSASSIASELCGFITILSGTVVLHDTRSSDPASVSEMYMSVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
            ANGDTWKRKSEEI LPDFDAILKQDHFT
Sbjct: 361 PANGDTWKRKSEEILLPDFDAILKQDHFT 353

BLAST of Carg25828 vs. ExPASy TrEMBL
Match: A0A1S3CF57 (Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103500043 PE=3 SV=1)

HSP 1 Score: 627.5 bits (1617), Expect = 3.8e-176
Identity = 331/389 (85.09%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           M+T++P ISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MTTYEPSISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGM TMIVGEFSNFVAY+YAPAILVTPLGAISIIVSAVLAHFFLKEKL+KM VLGC+L
Sbjct: 61  WWIGMITMIVGEFSNFVAYIYAPAILVTPLGAISIIVSAVLAHFFLKEKLQKMGVLGCIL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELA QPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELAIQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIY+GICSIIGSLTVMSIKAIGIAIKLTMEG SQVAHFQTWVF+MVAISCII+QLNYLNK
Sbjct: 181 LIYVGICSIIGSLTVMSIKAIGIAIKLTMEGWSQVAHFQTWVFLMVAISCIIIQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           AS IMFKDWSGQSASSIASELCGFVTILSGT+VLH TRSPD ASVSEMY+ VSPQVSWYF
Sbjct: 301 ASVIMFKDWSGQSASSIASELCGFVTILSGTVVLHDTRSPDSASVSEMYMSVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
            ANGDTWKRKSEEI LPDFDAILKQDHFT
Sbjct: 361 PANGDTWKRKSEEILLPDFDAILKQDHFT 353

BLAST of Carg25828 vs. ExPASy TrEMBL
Match: A0A6J1F2H7 (Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111439042 PE=3 SV=1)

HSP 1 Score: 624.0 bits (1608), Expect = 4.2e-175
Identity = 330/389 (84.83%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           M+T++P IS NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 1   MTTNEPSISANDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGM TMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKE+L+KM VLGCVL
Sbjct: 61  WWIGMITMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKERLQKMGVLGCVL 120

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIWELA QPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 121 CVVGSTMIVLHAPGERTPSSVDEIWELAIQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIY+GICSIIGSLTVMSIKAIGIAIKLT+EG SQVAHFQTWVFVMVAISCII+QLNYLNK
Sbjct: 181 LIYVGICSIIGSLTVMSIKAIGIAIKLTLEGWSQVAHFQTWVFVMVAISCIIIQLNYLNK 240

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 241 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 300

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           AS IMFKDWSGQSASSIASELCGFVTILSGT+VLH TRSPDPASVSEMY+ VSPQVSWYF
Sbjct: 301 ASVIMFKDWSGQSASSIASELCGFVTILSGTVVLHDTRSPDPASVSEMYMSVSPQVSWYF 353

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
            ANGDTWKR+SEE+ LPDFDAILKQDHFT
Sbjct: 361 PANGDTWKRQSEEMLLPDFDAILKQDHFT 353

BLAST of Carg25828 vs. ExPASy TrEMBL
Match: A0A6J1INJ5 (Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111477149 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 9.3e-175
Identity = 329/389 (84.58%), Postives = 342/389 (87.92%), Query Frame = 0

Query: 1   MSTHDPFISPNDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 60
           M+T++P IS NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL
Sbjct: 81  MTTNEPSISANDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPL 140

Query: 61  WWIGMSTMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVL 120
           WWIGM TMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKE+L+KM VLGCVL
Sbjct: 141 WWIGMITMIVGEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKERLQKMGVLGCVL 200

Query: 121 CVVGSTMIVLHAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 180
           CVVGSTMIVLHAPGERTPSSVDEIW+LA QPTFLLYTASVIAIVLFLVLYCEPRYGQTNI
Sbjct: 201 CVVGSTMIVLHAPGERTPSSVDEIWDLAIQPTFLLYTASVIAIVLFLVLYCEPRYGQTNI 260

Query: 181 LIYIGICSIIGSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNK 240
           LIY+GICSIIGSLTVMSIKAIGIAIKLT+EG SQVAHFQTWVFVMVAISCII+QLNYLNK
Sbjct: 261 LIYVGICSIIGSLTVMSIKAIGIAIKLTLEGWSQVAHFQTWVFVMVAISCIIIQLNYLNK 320

Query: 241 STFVASSYLPCDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIF 300
                                               ALDTFDTAVVSPIHYAMFTSFTIF
Sbjct: 321 ------------------------------------ALDTFDTAVVSPIHYAMFTSFTIF 380

Query: 301 ASAIMFKDWSGQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYF 360
           AS IMFKDWSGQSASSIASELCGFVTILSGT+VLH TRSPDPASVSEMY+ VSPQVSWYF
Sbjct: 381 ASVIMFKDWSGQSASSIASELCGFVTILSGTVVLHDTRSPDPASVSEMYMSVSPQVSWYF 433

Query: 361 QANGDTWKRKSEEIPLPDFDAILKQDHFT 390
            ANGDTWKR+SEE+ LPDFDAILKQDHFT
Sbjct: 441 PANGDTWKRQSEEMLLPDFDAILKQDHFT 433

BLAST of Carg25828 vs. TAIR 10
Match: AT2G21120.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 410.2 bits (1053), Expect = 1.8e-114
Identity = 219/362 (60.50%), Postives = 267/362 (73.76%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN KG +LA+ SS FIGSSFI+KK GL+RAGA G+RA  GGY YLLEPLWW GM TMIVG
Sbjct: 4   DNGKGLILAVASSVFIGSSFILKKKGLKRAGAIGTRAGYGGYTYLLEPLWWAGMVTMIVG 63

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NFVAY+YAPA+LVTPLGA+SII+SAVLAHF LKEKL+KM VLGCV C+VGS +IV+H
Sbjct: 64  EAANFVAYIYAPAVLVTPLGALSIIISAVLAHFLLKEKLKKMGVLGCVSCIVGSVVIVIH 123

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+TP+SV+EIW LATQP FL+Y A  ++IVL L+L+ EP  GQTNIL+YIGICS++G
Sbjct: 124 APKEQTPNSVEEIWNLATQPAFLIYVAITMSIVLALILHFEPLCGQTNILVYIGICSLMG 183

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           +LTVMSIKAIGIAIKLTMEG+SQ+ + QTW+FVMVA++C++ QL YLNK           
Sbjct: 184 ALTVMSIKAIGIAIKLTMEGVSQIGYPQTWLFVMVAVTCVVTQLIYLNK----------- 243

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALDTF+ A+VSP++Y MFT+ TI ASAIMFKDWSG
Sbjct: 244 -------------------------ALDTFNAAIVSPVYYVMFTTLTIVASAIMFKDWSG 303

Query: 312 QSASSIASELCGFVTILSGTMVLHATRSPDPASVSEMYLPVSPQVSWYFQANGDTWKRKS 371
           Q A+S+ASELCGF+T+L+GTM+LH TR  +    S      S  V WY     D+ K  +
Sbjct: 304 QDAASVASELCGFITVLTGTMILHGTREEEQQQAS------SEHVRWY-----DSRKSMN 318

Query: 372 EE 374
           EE
Sbjct: 364 EE 318

BLAST of Carg25828 vs. TAIR 10
Match: AT4G38730.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 390.6 bits (1002), Expect = 1.5e-108
Identity = 202/338 (59.76%), Postives = 252/338 (74.56%), Query Frame = 0

Query: 11  NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIV 70
           +DN  G +LA+ SS FIGSSFI+KK GL+RA A+G+RA  GGY YLLEPLWW+G+ TM  
Sbjct: 3   SDNEMGLVLAVSSSVFIGSSFILKKKGLKRAAANGTRAGFGGYTYLLEPLWWVGLVTMTF 62

Query: 71  GEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVL 130
           GE +NFVAYVYAPA+LVTPLGA+SII+SAVLAHF L EKL KM V GCV C+VGS MIV+
Sbjct: 63  GEIANFVAYVYAPAVLVTPLGALSIIISAVLAHFLLDEKLRKMGVWGCVCCIVGSVMIVI 122

Query: 131 HAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSII 190
           HAP E+TP+SV+EIW+LA QP FL+Y A  ++IVL L+LYCEP  GQTNIL+YIGICS++
Sbjct: 123 HAPQEQTPNSVEEIWKLAMQPAFLIYVAISMSIVLALILYCEPLCGQTNILVYIGICSLM 182

Query: 191 GSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLP 250
           GSLTVMSIKA+GIAIKLT EG++Q+ + +TW F MVA  C+++Q+ YLNK          
Sbjct: 183 GSLTVMSIKAVGIAIKLTFEGINQIWYPETWFFAMVAAICVVMQMIYLNK---------- 242

Query: 251 CDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWS 310
                                     ALDTF+ A+VSPI+Y MFT+ TI ASAIMFKDW+
Sbjct: 243 --------------------------ALDTFNAAIVSPIYYVMFTTLTIVASAIMFKDWN 302

Query: 311 GQSASSIASELCGFVTILSGTMVLHATRSPDPASVSEM 349
           GQ+  SIASE+CGF+T+L+GT++LH+TR  + AS   M
Sbjct: 303 GQNTDSIASEICGFITVLTGTVILHSTREEEQASPRRM 304

BLAST of Carg25828 vs. TAIR 10
Match: AT3G23870.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 370.5 bits (950), Expect = 1.6e-102
Identity = 190/327 (58.10%), Postives = 237/327 (72.48%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN+ G +LA+ SS FIGSSFIIKK GL++AGASG RA  GGYGYL EP WW GM TMIVG
Sbjct: 7   DNINGVILAVSSSIFIGSSFIIKKKGLKKAGASGVRAGEGGYGYLKEPWWWAGMITMIVG 66

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NF AY +APAILVTPLGA+SII SAVLAHF LKEKL    +LGC+LCVVGST IVLH
Sbjct: 67  EVANFAAYAFAPAILVTPLGALSIIFSAVLAHFILKEKLHMFGILGCILCVVGSTTIVLH 126

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+   SV +IW+LA +P FL+Y+A ++ +V  L+ Y EPRYG+T++++Y+GICS++G
Sbjct: 127 APHEQKIESVKQIWQLAIEPGFLVYSAVIVIVVAILIFYYEPRYGKTHMIVYVGICSLMG 186

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           SLTVMS+KA+ IAIKLT  G +Q  +F TW+F++V  +C I+Q+NYLNK           
Sbjct: 187 SLTVMSVKAVAIAIKLTFSGTNQFKYFNTWIFILVVATCCILQINYLNK----------- 246

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALDTF+TAV+SP++Y MFT+FTI AS IMFKDW+ 
Sbjct: 247 -------------------------ALDTFNTAVISPVYYVMFTTFTIIASMIMFKDWAS 297

Query: 312 QSASSIASELCGFVTILSGTMVLHATR 339
           QS   IA+ELCGFVTILSGT +LH T+
Sbjct: 307 QSGLKIATELCGFVTILSGTFLLHKTK 297

BLAST of Carg25828 vs. TAIR 10
Match: AT1G34470.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 367.9 bits (943), Expect = 1.0e-101
Identity = 200/383 (52.22%), Postives = 255/383 (66.58%), Query Frame = 0

Query: 11  NDNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIV 70
           +DN+KG +LA+ SS FIG+SFI+KK GL+RAGASG RA SGGY YLLEPLWW+GM TMIV
Sbjct: 17  SDNIKGLVLALSSSLFIGASFIVKKKGLKRAGASGLRAGSGGYSYLLEPLWWVGMITMIV 76

Query: 71  GEFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVL 130
           GE +NF AY +APAILVTPLGA+SII+SA LAH  L EKL    +LGCVLCVVGS  IVL
Sbjct: 77  GEIANFAAYAFAPAILVTPLGALSIIISAALAHVILHEKLHTFGLLGCVLCVVGSITIVL 136

Query: 131 HAPGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSII 190
           HAP E+   SV ++W LAT+P FLLY A+V+   + L++   P+YGQ+++++YIG+CS++
Sbjct: 137 HAPQEQEIDSVLQVWNLATEPAFLLYAAAVVGAAIILIVQFVPQYGQSHVMVYIGVCSLV 196

Query: 191 GSLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLP 250
           GSL+VMS+KA+GIA+KLT  G++Q+ + QTWVF ++ ++C+I Q+NYLNK          
Sbjct: 197 GSLSVMSVKALGIALKLTFSGMNQLIYPQTWVFTLIVLTCVITQMNYLNK---------- 256

Query: 251 CDVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWS 310
                                     ALDTF+TAVVSPI+Y MFTS TI AS IMFKDW 
Sbjct: 257 --------------------------ALDTFNTAVVSPIYYVMFTSLTILASVIMFKDWD 316

Query: 311 GQSASSIASELCGFVTILSGTMVLHATRSPDPASVS----EMYLPVSPQVSWYFQANGDT 370
            Q  + I +ELCGFVTILSGT +LH T+     S S     + LP   + S  F+  G  
Sbjct: 317 RQDGTQIVTELCGFVTILSGTFLLHKTKDMVDGSSSLGNLALRLPKQLEDSNGFEQEGIP 363

Query: 371 WKRKSEEI---PLPDFDAILKQD 387
              +  E    P P    IL QD
Sbjct: 377 LTLRRHECTKSPRPMRQFILPQD 363

BLAST of Carg25828 vs. TAIR 10
Match: AT4G13800.1 (Protein of unknown function (DUF803) )

HSP 1 Score: 366.7 bits (940), Expect = 2.3e-101
Identity = 189/327 (57.80%), Postives = 235/327 (71.87%), Query Frame = 0

Query: 12  DNLKGFLLAMLSSAFIGSSFIIKKLGLRRAGASGSRASSGGYGYLLEPLWWIGMSTMIVG 71
           DN+ G +LA+ SS FIGSSFIIKK GL++AG SG+RA  GGYGYL EP WW GM TMIVG
Sbjct: 7   DNIHGVILAVSSSIFIGSSFIIKKKGLKKAGVSGARAGEGGYGYLYEPWWWAGMITMIVG 66

Query: 72  EFSNFVAYVYAPAILVTPLGAISIIVSAVLAHFFLKEKLEKMDVLGCVLCVVGSTMIVLH 131
           E +NF AY +APAILVTPLGA+SII SAVLAHF L+EKL    +LGCVLCVVGST IVLH
Sbjct: 67  EIANFAAYAFAPAILVTPLGALSIIFSAVLAHFILEEKLHMFGILGCVLCVVGSTTIVLH 126

Query: 132 APGERTPSSVDEIWELATQPTFLLYTASVIAIVLFLVLYCEPRYGQTNILIYIGICSIIG 191
           AP E+   SV ++W LAT+P FL Y+A V+ +VL L+ Y EPRYG+T++++Y+GICS++G
Sbjct: 127 APHEQGIESVKQVWHLATEPGFLAYSAVVLVVVLALIFYYEPRYGKTHMIVYVGICSLMG 186

Query: 192 SLTVMSIKAIGIAIKLTMEGLSQVAHFQTWVFVMVAISCIIVQLNYLNKSTFVASSYLPC 251
           SLTVMS+KA+ IAIKLT  G++Q  +F  W+F++V   C I+Q+NYLNK           
Sbjct: 187 SLTVMSVKAVAIAIKLTFSGMNQFKYFHAWIFIIVVTICCILQINYLNK----------- 246

Query: 252 DVFIVSLWLLLSFVCFTRKRSLHEQALDTFDTAVVSPIHYAMFTSFTIFASAIMFKDWSG 311
                                    ALD F+TAV+SP++Y MFT+FTI AS IMFKDW+ 
Sbjct: 247 -------------------------ALDNFNTAVISPVYYVMFTTFTILASMIMFKDWAS 297

Query: 312 QSASSIASELCGFVTILSGTMVLHATR 339
           QS   IA+ELCGFVTILSGT +LH T+
Sbjct: 307 QSGLQIATELCGFVTILSGTFLLHKTK 297

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029939.18.5e-215100.00putative magnesium transporter NIPA6 [Cucurbita argyrosperma subsp. argyrosperma... [more]
KAG6607605.16.8e-18890.75putative magnesium transporter NIPA6, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_023520888.15.8e-18790.49probable magnesium transporter NIPA6 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022932049.11.7e-18690.23probable magnesium transporter NIPA6 isoform X1 [Cucurbita moschata][more]
XP_038897340.13.1e-18087.18probable magnesium transporter NIPA6 isoform X1 [Benincasa hispida] >XP_03889734... [more]
Match NameE-valueIdentityDescription
Q8GWX22.6e-11360.50Probable magnesium transporter NIPA6 OS=Arabidopsis thaliana OX=3702 GN=At2g2112... [more]
Q8GYS12.1e-10759.76Probable magnesium transporter NIPA7 OS=Arabidopsis thaliana OX=3702 GN=At4g3873... [more]
Q9LIR92.3e-10158.10Probable magnesium transporter NIPA1 OS=Arabidopsis thaliana OX=3702 GN=At3g2387... [more]
Q9LNK71.5e-10052.22Probable magnesium transporter NIPA3 OS=Arabidopsis thaliana OX=3702 GN=At1g3447... [more]
B3LFA33.3e-10057.80Probable magnesium transporter NIPA2 OS=Arabidopsis thaliana OX=3702 GN=At4g1380... [more]
Match NameE-valueIdentityDescription
A0A6J1EVY48.1e-18790.23Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111438382 PE=... [more]
A0A0A0K4P79.9e-17785.09Probable magnesium transporter OS=Cucumis sativus OX=3659 GN=Csa_7G291140 PE=3 S... [more]
A0A1S3CF573.8e-17685.09Probable magnesium transporter OS=Cucumis melo OX=3656 GN=LOC103500043 PE=3 SV=1[more]
A0A6J1F2H74.2e-17584.83Probable magnesium transporter OS=Cucurbita moschata OX=3662 GN=LOC111439042 PE=... [more]
A0A6J1INJ59.3e-17584.58Probable magnesium transporter OS=Cucurbita maxima OX=3661 GN=LOC111477149 PE=3 ... [more]
Match NameE-valueIdentityDescription
AT2G21120.11.8e-11460.50Protein of unknown function (DUF803) [more]
AT4G38730.11.5e-10859.76Protein of unknown function (DUF803) [more]
AT3G23870.11.6e-10258.10Protein of unknown function (DUF803) [more]
AT1G34470.11.0e-10152.22Protein of unknown function (DUF803) [more]
AT4G13800.12.3e-10157.80Protein of unknown function (DUF803) [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008521Magnesium transporter NIPAPFAMPF05653Mg_trans_NIPAcoord: 258..338
e-value: 3.2E-23
score: 82.3
coord: 12..242
e-value: 2.7E-100
score: 335.3
IPR008521Magnesium transporter NIPAPANTHERPTHR12570UNCHARACTERIZEDcoord: 11..389
NoneNo IPR availablePANTHERPTHR12570:SF25MAGNESIUM TRANSPORTER-RELATEDcoord: 11..389
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 59..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25828-RACarg25828-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1903830 magnesium ion transmembrane transport
biological_process GO:0015693 magnesium ion transport
cellular_component GO:0005769 early endosome
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0015095 magnesium ion transmembrane transporter activity