Carg25820 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25820
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	aspartic proteinase-like protein 2
Location	Carg_Chr05: 5965855 .. 5970924 (-)
RNA-Seq Expression	Carg25820
Synteny	Carg25820

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CCAAATCCAAGTCCAAATTTACTGTTTATTTGATTATTCAAAATCAAGTGAAGATTGCGACCAATTCTTTTCTCTTGGCTCAACGCGGTTCATTCTATTCCCCTGTTTCTAATTTTGTTTCTTCACCTTATTCTCTACTTCGAAGAGTTTTCTAGGTACACCCATTGCTGTTAGCCCATAAATCTGAGCTGGGCGTCGTTTTTTTTTTTCAAATTAGGTTCTGGGTTTTGCTTGAATCTCTGTTCTATTTGTTGGGGGTTTGGTTTTGATTATGGAGATTGCAAGACTTGCCGTAGTGAGCTTCTTGCTGGCGATTTCGTTTTTGTCAACTGGGGATTGCAATTTGGTGTTCAATGTTCATCACAAGTTCAAGGGCCGCGAGAGGTCCTTGGAAGCCTTCAAAGCCCACGATGTTCTCCGTCGCGGTAGATTTCTTTCTGCTATTGATCTCAACTTGGGCGGCAACGGACACCCTTCTGAATCTGGGTTAGTCTTGATTTCTGTTGTGGTTATTTAGCATACCTGAAATTTCTGTCGTTTTTGTGCGGTGATCTTGTATGTAACTGCTTGTTTAGTGGACAATTTACTACATATCTCAGGTATTCCGTTGTGATCTTTCAAACAAGACCTGTATGATTGGATCCTGTTCTACCTCTTTTAAGTACATACCATATGGAATTTGGATCTTTACCTTTTTTGTTTTGACTTTATGAAATCACTGCTCAAGTTTTTAGTTGCTTATGGATTTGGATTATTGTTCCTCGTTTTAATGGATTTGTTCGAGTCGGCCTGTTCCTTTTTCTTGTTTGATAAAAGAGAGTATTATTGCTCACTTTATCATATTCGTTGTTGGATGGTGGAAACTGGAAATACTTATATGTTCGATCTTCTCTATCGGTTGGATTAGGCTGTATTTCGCTAAAATCGGGCTTGGGACACCAGCAAAGGACTATTATGTACAAGTGGATACAGGAAGTGACCTCCTATGGGTCAATTGTGCAGGCTGTACAAACTGTCCAAGGAAAAGTGATCTTGGTGTAAGTCTCTTGAACTTTAGATTCTTAATTATATTCAGAACTACATCAAACATTTGTCGCTTGTCATCGTCTTCGATGGACCAAAGCTTCACAAGCAATACTCTGATTATGTTACAGATAGAACTGACACTATACAATCCATCGAGCTCCAGTACTTCAAACTTGGTAACTTGTGGTCAAGATTTTTGCACTTCCACATACGACGGTCCGATTCCTGGCTGTAGGCCTGAACTACTCTGTGAATATAAAGTTGCATATGGAGATGGAAGCTCAACTACAGGATATTTTGTGAAGGATCATGTAGTACTCGATCGAGTGACGGGAAATTTTCAAACTGAATCTACAAATGGGAGTATAGTATTTGGGTAAGAAAAGCATTTTCTGCATTTTCTACATTTTCTTTTATTTTTGCTCTGTGTCCATCAATTGATCTTGTGGGATTATATCGAAAAGTTCAAGTTCAAGTTCTTTTATATGAATTTCTATTTCATGGCATCATGAAATTTTATACATCTACAATGAATTAGTGAAAAAATGTTTTTAGATAACTATTAGTAAACCTAACGAGTCGTCTGTTTGACGTTTGACGTATTTTATAGTATATATTGTATATTTAACGATGAATAAAGTATCTACCAAGGAAGAATCGTTATTATTTCCCATTCTCCTCTCAAGTTGTTCCTCTGCTTTAGTTATTTGTTTCGACATATTTCGATCATGGATAATTTTCCACACCTACTCAATGACTTTGTGAAATGCATCGATCATTCTGGTTTTCTGTTTTTTTTTTCTTTTTCGTTCATACTTATTTTGTCTTCCTTGATGGACGGACGGATAGTTGTGGCGCTCAACAATCTGGTCAACTCGGTGCAACATCTGCAGCACTTGATGGGATACTTGGTTTCGGACAAGCAAATTCATCCATGATTTCGCAGCTGGCTTCATCAGGAAAAATTAAAAGAATTTTTGCACATTGCTTGGATAATATTAATGGAGGTGGAATTTTCGCCATTGGAGAAGTGGTGCAGCCAAAAGTTCGCACCACTCCATTAGTGCAGCAACAGTATGTTACACTTCCTTGCAATTAGTACTTTTCGACACCTTCAATCGACAACGTTCTTCTTCCTTTTTTTAAGTAGTAGCCTTAGACTTAGTTATTTCATGGTGCACTGTAGGGCACATTACAATGTGTTTATGAAGGCAATTGAGGTTGGCAATGAAGTGCTGAATCTCCCCACGGATGTTTTCGACACTGATTTAAAGAAAGGAACAATTATCGACAGTGGCACGACGTTGGCTTATTTACCAGATGTGATTTATGAACCATTGATAGCGAAGGTACACTATATAAAAAATATTATTTCTAGTTTTAAGTTTGATAGCGTTAAGTGTGTTTCAGAAACTGAAGCTGTATTTACTATGCTAGATTTTTGCAAGGCAGACTGGATTGAAGTTGCATACTGTTGAAGAACAGTTCACCTGCTTCGAATACGACGGAAAGTAAGATGGCATGATACCCTACCTGGCTAAGTTCTATCGCTCTTCGTCGAACATTTAAAATAGCGATCTCGTTGTTCTGGAATCGATATTCAATTAGGGATCCTCCTTTTACAAGATTGTTAAATGTGTTGCTAGTTTGTCCTCTTAATTTGGTCTGGTTTTTATTGATTCATGAGTAAAGATTTATTAGCTTTATCTTAGGAGATACCCTCTAGGATCCCGGGTTCATGTTCATGTCGAAAACTTAATAACTCGAAACTTCGTTATCCCTAAGAGTTGGTCTTGGTATAAGAGAAGCCTTTCGGGACGTGATCACGAGCATAATAATGTAAGAAACGGGGCCCTTGACAAGAGTTTAGCATGCTGCATAGTATTCTTTACCTGATGAAAACATTCTTTACCTGCATAGTGTTCACTATCTGGAAATTTCTTAAGTTTCTCATTTGGGATTTATGTTCTTCATGCAGTGTTGACGATGGATTTCCTACTATTACATTTCATTTCGAAGATTCGCTATCTTTGACCGTTCATCCTCACGAGTATCTATTTGATATTGCGGTGAGTGAAATGAGCTTGGAACGACAAGCATCTTAGCAACTAGTTTTTAAATCAAATAATTGATATTACTTAGGAAGATCAAATTTGAAAATCTCTGCGTGTAAAGGGCGATACCGATAACGATACCGATACCGATACCGGGGTAGCCTTAAAAGCGTATTTACAGAAACTTCCCTGACATAATTTTTTGTTAACCTTTCTGATCAAATTTGCAGAGTAATAGATGGTGCGTCGGTTGGCAGAACAGTGGTGCCCAATCTAGAGATGGAAAGGATATGATTCTGTTGGGAGGTCAGTGAAAAGACAATGAACATTTCTATTATATTTCATCGCTTCTGCTCATGAAACTTCGTCATCTGTGACGGCCTAGGCCCACCGTTAGTAGATATTGTCCTCTTTGGGCTTTCCCTTTCGAGTTTCCCTCAAAACTTTGAAATGCGTCTGCTAGGGAAAAGTTTTCACACCCTTATAAAGAGTGTTTTGTTCGGGGATCTCACATTATCACTCCCTTAAGACTTGAAACTTGAGTGAATCCCTACTTTAATACTACACCGTTGAACACTCCGACACTTTAGTTTGGCTAGCATGTCCATGTCTAACACATGTCACACTTGAACACTCCCAACACTTGTTAGCACAATGAATGTGTTAGACACTAGCGGTACAAAGTTGATATACGTCCAATGTTTATTAAACACATAACAAACACATAGAATTTGAATAAATTTCGAGAGTGAACGGTATTAAGTTCATTTTTTTGAATTTTCTTGTATGAAAATGATATATTTTAACATGTTCGTGTTGTGTTGTATCGTATCCATGCTTCTTAGTAACCACCCCCAAGATATAAAACTAAAACGGTGTTCCTTTTCGGGATGTGCATATTATTTTTAATCCGTTTTTCCCATTTGTTTCTGATTTCAGATTTGGTGCTTCAAAATAGACTCGTACTGTACGACTTGGAAAATCAGACCATCGGCTGGACGGAGTATAATTGTGAGTTATTTATTTAGCACGGAGTATAACTGTGAGTTATTTATTTAGCATTAGAAAACTTTACATAACAGCCTAATCTCACAACTAGCCGATATCGTCCTCTTTGGGCTTTTCCTTTCGGGCTTCCCCTCAAGGTTTTTAAAACGCATCTATTAGGGAGAGGTTTTCACACCCGTATAAAGAATGTTTCGTTCCCCTCTCCCAACCGATGTCGTATTTCACAATCCACCCTCCTTCAGGGCCCAGCTCCTCACTAACACAAGTGATCTAAGAACGGACCCCAATCCAAATTTGATAATAAGAGTAGGAAAACTAGAAAAGTCGGTTCGTGTCGTTAATATCTTGATCGAAACAGCAGTGCGACAGGTTGGGTAGAGTAATAAATAATGAAAAGTTAGATTCCTTTTGCTCACATGGCCTCCTTAGTGTATTAAATATTCAGAAATTGGTGTGAATCTGTTCCAATGGGGCATTGTTGCAGGCTCTTCAAGCATTAAGGTGAGGGATGAACACAGTGGAGCCATATACACAGTGGGGCCTCATAACCTTTCTTCAGCTTCTACTGTAAGAGTTGGAACAATATCATCAATGCTGCTCTCAATACTTCTACTGCTTACTGTTCTTCATTCTTTTACAAATTAAAAGGGATTCTTTTCAAACTCGTATCAATTTTTTCAATGTACATATTATGCTCTGCTGCCCATTTCATTCTTTTTCATTCTTTAACATCATCCATTTGGAAGTACTGAATGATTCTTTGGGCGTTTGTGAAAAACTTGTATCGTTCTTATTGATATTCATAACTTCAACTGTCTTTCCTTTAACATCTGCTCATTGGCCTCAACTATGGAAACAATATTGTAATGTTTATTGGTGTATTATTGATCGAGTGTTTAGTTCACTGTTTTGGGAGCGGAGAGTGTTGGATGAAGTCTCACGTCGACTCATTTAGGGAATGATCTTGGGTTTATAATCAATT

mRNA sequence

CCAAATCCAAGTCCAAATTTACTGTTTATTTGATTATTCAAAATCAAGTGAAGATTGCGACCAATTCTTTTCTCTTTCTCTACTTCGAAGAGTTTTCTAGGTACACCCATTGCTGTTAGCCCATAAATCTGAGCTGGGCGTCGTTTTTTTTTTTCAAATTAGGTTCTGGGTTTTGCTTGAATCTCTGTTCTATTTGTTGGGGGTTTGGTTTTGATTATGGAGATTGCAAGACTTGCCGTAGTGAGCTTCTTGCTGGCGATTTCGTTTTTGTCAACTGGGGATTGCAATTTGGTGTTCAATGTTCATCACAAGTTCAAGGGCCGCGAGAGGTCCTTGGAAGCCTTCAAAGCCCACGATGTTCTCCGTCGCGGTAGATTTCTTTCTGCTATTGATCTCAACTTGGGCGGCAACGGACACCCTTCTGAATCTGGGCTGTATTTCGCTAAAATCGGGCTTGGGACACCAGCAAAGGACTATTATGTACAAGTGGATACAGGAAGTGACCTCCTATGGGTCAATTGTGCAGGCTGTACAAACTGTCCAAGGAAAAGTGATCTTGGTATAGAACTGACACTATACAATCCATCGAGCTCCAGTACTTCAAACTTGGTAACTTGTGGTCAAGATTTTTGCACTTCCACATACGACGGTCCGATTCCTGGCTGTAGGCCTGAACTACTCTGTGAATATAAAGTTGCATATGGAGATGGAAGCTCAACTACAGGATATTTTGTGAAGGATCATGTAGTACTCGATCGAGTGACGGGAAATTTTCAAACTGAATCTACAAATGGGAGTATAGTATTTGGTTGTGGCGCTCAACAATCTGGTCAACTCGGTGCAACATCTGCAGCACTTGATGGGATACTTGGTTTCGGACAAGCAAATTCATCCATGATTTCGCAGCTGGCTTCATCAGGAAAAATTAAAAGAATTTTTGCACATTGCTTGGATAATATTAATGGAGGTGGAATTTTCGCCATTGGAGAAGTGGTGCAGCCAAAAGTTCGCACCACTCCATTAGTGCAGCAACATAGCCTTAGACTTAGTTATTTCATGGTGCACTGTAGGGCACATTACAATGTGTTTATGAAGGCAATTGAGGTTGGCAATGAAGTGCTGAATCTCCCCACGGATGTTTTCGACACTGATTTAAAGAAAGGAACAATTATCGACAGTGGCACGACGTTGGCTTATTTACCAGATGTGATTTATGAACCATTGATAGCGAAGATTTTTGCAAGGCAGACTGGATTGAAGTTGCATACTGTTGAAGAACAGTTCACCTGCTTCGAATACGACGGAAATGTTGACGATGGATTTCCTACTATTACATTTCATTTCGAAGATTCGCTATCTTTGACCGTTCATCCTCACGAGTATCTATTTGATATTGCGAGTAATAGATGGTGCGTCGGTTGGCAGAACAGTGGCTCTTCAAGCATTAAGGTGAGGGATGAACACAGTGGAGCCATATACACAGTGGGGCCTCATAACCTTTCTTCAGCTTCTACTGTAAGAGTTGGAACAATATCATCAATGCTGCTCTCAATACTTCTACTGCTTACTGTTCTTCATTCTTTTACAAATTAAAAGGGATTCTTTTCAAACTCGTATCAATTTTTTCAATGTACATATTATGCTCTGCTGCCCATTTCATTCTTTTTCATTCTTTAACATCATCCATTTGGAAGTACTGAATGATTCTTTGGGCGTTTGTGAAAAACTTGTATCGTTCTTATTGATATTCATAACTTCAACTGTCTTTCCTTTAACATCTGCTCATTGGCCTCAACTATGGAAACAATATTGTAATGTTTATTGGTGTATTATTGATCGAGTGTTTAGTTCACTGTTTTGGGAGCGGAGAGTGTTGGATGAAGTCTCACGTCGACTCATTTAGGGAATGATCTTGGGTTTATAATCAATT

Coding sequence (CDS)

ATGGAGATTGCAAGACTTGCCGTAGTGAGCTTCTTGCTGGCGATTTCGTTTTTGTCAACTGGGGATTGCAATTTGGTGTTCAATGTTCATCACAAGTTCAAGGGCCGCGAGAGGTCCTTGGAAGCCTTCAAAGCCCACGATGTTCTCCGTCGCGGTAGATTTCTTTCTGCTATTGATCTCAACTTGGGCGGCAACGGACACCCTTCTGAATCTGGGCTGTATTTCGCTAAAATCGGGCTTGGGACACCAGCAAAGGACTATTATGTACAAGTGGATACAGGAAGTGACCTCCTATGGGTCAATTGTGCAGGCTGTACAAACTGTCCAAGGAAAAGTGATCTTGGTATAGAACTGACACTATACAATCCATCGAGCTCCAGTACTTCAAACTTGGTAACTTGTGGTCAAGATTTTTGCACTTCCACATACGACGGTCCGATTCCTGGCTGTAGGCCTGAACTACTCTGTGAATATAAAGTTGCATATGGAGATGGAAGCTCAACTACAGGATATTTTGTGAAGGATCATGTAGTACTCGATCGAGTGACGGGAAATTTTCAAACTGAATCTACAAATGGGAGTATAGTATTTGGTTGTGGCGCTCAACAATCTGGTCAACTCGGTGCAACATCTGCAGCACTTGATGGGATACTTGGTTTCGGACAAGCAAATTCATCCATGATTTCGCAGCTGGCTTCATCAGGAAAAATTAAAAGAATTTTTGCACATTGCTTGGATAATATTAATGGAGGTGGAATTTTCGCCATTGGAGAAGTGGTGCAGCCAAAAGTTCGCACCACTCCATTAGTGCAGCAACATAGCCTTAGACTTAGTTATTTCATGGTGCACTGTAGGGCACATTACAATGTGTTTATGAAGGCAATTGAGGTTGGCAATGAAGTGCTGAATCTCCCCACGGATGTTTTCGACACTGATTTAAAGAAAGGAACAATTATCGACAGTGGCACGACGTTGGCTTATTTACCAGATGTGATTTATGAACCATTGATAGCGAAGATTTTTGCAAGGCAGACTGGATTGAAGTTGCATACTGTTGAAGAACAGTTCACCTGCTTCGAATACGACGGAAATGTTGACGATGGATTTCCTACTATTACATTTCATTTCGAAGATTCGCTATCTTTGACCGTTCATCCTCACGAGTATCTATTTGATATTGCGAGTAATAGATGGTGCGTCGGTTGGCAGAACAGTGGCTCTTCAAGCATTAAGGTGAGGGATGAACACAGTGGAGCCATATACACAGTGGGGCCTCATAACCTTTCTTCAGCTTCTACTGTAAGAGTTGGAACAATATCATCAATGCTGCTCTCAATACTTCTACTGCTTACTGTTCTTCATTCTTTTACAAATTAA

Protein sequence

MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDLNLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSGSSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSMLLSILLLLTVLHSFTN

Homology

[Close]

BLAST of Carg25820 vs. NCBI nr
Match: KAG7029946.1 (Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 929.1 bits (2400), Expect = 1.5e-266
Identity = 458/458 (100.00%), Postives = 458/458 (100.00%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSGSSSIKVRDEHSGAI 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSGSSSIKVRDEHSGAI
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSGSSSIKVRDEHSGAI 420

Query: 421 YTVGPHNLSSASTVRVGTISSMLLSILLLLTVLHSFTN 459
           YTVGPHNLSSASTVRVGTISSMLLSILLLLTVLHSFTN
Sbjct: 421 YTVGPHNLSSASTVRVGTISSMLLSILLLLTVLHSFTN 458

[Close]

BLAST of Carg25820 vs. NCBI nr
Match: KAG6598979.1 (Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 872.8 bits (2254), Expect = 1.3e-249
Identity = 444/496 (89.52%), Postives = 446/496 (89.92%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVGNE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. NCBI nr
Match: XP_022946441.1 (aspartic proteinase-like protein 2 [Cucurbita moschata])

HSP 1 Score: 866.7 bits (2238), Expect = 9.0e-248
Identity = 441/496 (88.91%), Postives = 443/496 (89.31%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           M IARLAVVSFLL IS LSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MAIARLAVVSFLLVISLLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVGNE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. NCBI nr
Match: XP_023547349.1 (aspartic proteinase-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 865.5 bits (2235), Expect = 2.0e-247
Identity = 440/496 (88.71%), Postives = 443/496 (89.31%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIARLAVVSFLL ISFLS GDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MEIARLAVVSFLLVISFLSIGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVGNE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNLSSA +VRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLSSAPSVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. NCBI nr
Match: XP_022972843.1 (aspartic proteinase-like protein 2 [Cucurbita maxima])

HSP 1 Score: 857.1 bits (2213), Expect = 7.1e-245
Identity = 436/496 (87.90%), Postives = 442/496 (89.11%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIARLAVVSFLL IS LSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MEIARLAVVSFLLVISLLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNF+TESTNGSIVFGCGAQQS QLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFKTESTNGSIVFGCGAQQSVQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVG+E
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGDE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDL+KGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLRKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNL SAS+VRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLYSASSVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. ExPASy Swiss-Prot
Match: Q4V3D2 (Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 6.7e-121
Identity = 235/495 (47.47%), Postives = 306/495 (61.82%), Query Frame = 0


Query: 3   IARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDLNL 62
           I+R+  V F+L I  +S    N VFNV HKF G+E+ L   K+HD  R  R L+ IDL L
Sbjct: 10  ISRIVAVVFVLVIQVVSG---NFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPL 69

Query: 63  GGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYN 122
           GG+      GLYF KI LG+P K+YYVQVDTGSD+LWVNCA C  CP K+DLGI L+LY+
Sbjct: 70  GGDSRADSIGLYFTKIKLGSPPKEYYVQVDTGSDILWVNCAPCPKCPVKTDLGIPLSLYD 129

Query: 123 PSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRV 182
             +SSTS  V C  DFC+        G +    C Y V YGDGS++ G F+KD++ L++V
Sbjct: 130 SKTSSTSKNVGCEDDFCSFIMQSETCGAKKP--CSYHVVYGDGSTSDGDFIKDNITLEQV 189

Query: 183 TGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFA 242
           TGN +T      +VFGCG  QSGQLG T +A+DGI+GFGQ+N+S+ISQLA+ G  KRIF+
Sbjct: 190 TGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFS 249

Query: 243 HCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVL 302
           HCLDN+NGGGIFA+GEV  P V+TTP+V              + HYNV +K ++V  + +
Sbjct: 250 HCLDNMNGGGIFAVGEVESPVVKTTPIVPN------------QVHYNVILKGMDVDGDPI 309

Query: 303 NLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYD 362
           +LP  +  T+   GTIIDSGTTLAYLP  +Y  LI KI A+Q  +KLH V+E F CF + 
Sbjct: 310 DLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKITAKQQ-VKLHMVQETFACFSFT 369

Query: 363 GNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG---------------- 422
            N D  FP +  HFEDSL L+V+PH+YLF +  + +C GWQ+ G                
Sbjct: 370 SNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLV 429

Query: 423 ----------------------SSSIKVRDEHSGAIYTVGPHNL-SSASTVRVGTISSML 459
                                 SSSIKV+D  SGA Y +G  NL S+AS+V  GT+ ++L
Sbjct: 430 LSNKLVVYDLENEVIGWADHNCSSSIKVKD-GSGAAYQLGAENLISAASSVMNGTLVTLL 482

[Close]

BLAST of Carg25820 vs. ExPASy Swiss-Prot
Match: Q9S9K4 (Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2)

HSP 1 Score: 402.5 bits (1033), Expect = 6.3e-111
Identity = 213/491 (43.38%), Postives = 297/491 (60.49%), Query Frame = 0


Query: 1   MEIAR---LAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSA 60
           ME+ R   + V  F++ I F S    N VF   HKF G++++LE FK+HD  R  R L++
Sbjct: 1   MELRRKLCIVVAVFVIVIEFAS---ANFVFKAQHKFAGKKKNLEHFKSHDTRRHSRMLAS 60

Query: 61  IDLNLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIE 120
           IDL LGG+      GLYF KI LG+P K+Y+VQVDTGSD+LW+NC  C  CP K++L   
Sbjct: 61  IDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNLNFR 120

Query: 121 LTLYNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHV 180
           L+L++ ++SSTS  V C  DFC  ++      C+P L C Y + Y D S++ G F++D +
Sbjct: 121 LSLFDMNASSTSKKVGCDDDFC--SFISQSDSCQPALGCSYHIVYADESTSDGKFIRDML 180

Query: 181 VLDRVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKI 240
            L++VTG+ +T      +VFGCG+ QSGQLG   +A+DG++GFGQ+N+S++SQLA++G  
Sbjct: 181 TLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDA 240

Query: 241 KRIFAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEV 300
           KR+F+HCLDN+ GGGIFA+G V  PKV+TTP+V              + HYNV +  ++V
Sbjct: 241 KRVFSHCLDNVKGGGIFAVGVVDSPKVKTTPMVPN------------QMHYNVMLMGMDV 300

Query: 301 GNEVLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFT 360
               L+LP  +       GTI+DSGTTLAY P V+Y+ LI  I ARQ  +KLH VEE F 
Sbjct: 301 DGTSLDLPRSIVRNG---GTIVDSGTTLAYFPKVLYDSLIETILARQP-VKLHIVEETFQ 360

Query: 361 CFEYDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG----------- 420
           CF +  NVD+ FP ++F FEDS+ LTV+PH+YLF +    +C GWQ  G           
Sbjct: 361 CFSFSTNVDEAFPPVSFEFEDSVKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVIL 420

Query: 421 ---------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTI 451
                                      SSSIK++D  SG +Y+VG  NLSSA  + + T 
Sbjct: 421 LGDLVLSNKLVVYDLDNEVIGWADHNCSSSIKIKD-GSGGVYSVGADNLSSAPRLLMITK 469

[Close]

BLAST of Carg25820 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.5e-30
Identity = 103/352 (29.26%), Postives = 157/352 (44.60%), Query Frame = 0


Query: 65  NGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYNPS 124
           +G    SG YF++IG+GTPAK+ Y+ +DTGSD+ W+ C  C +C ++SD      ++NP+
Sbjct: 153 SGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSD-----PVFNPT 212

Query: 125 SSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRVTG 184
           SSST   +TC    C+         CR    C Y+V+YGDGS T G    D       T 
Sbjct: 213 SSSTYKSLTCSAPQCSLL---ETSACRSN-KCLYQVSYGDGSFTVGELATD-------TV 272

Query: 185 NFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFAHC 244
            F       ++  GCG    G    T AA  G+LG G    S+ +Q+ ++      F++C
Sbjct: 273 TFGNSGKINNVALGCGHDNEGLF--TGAA--GLLGLGGGVLSITNQMKATS-----FSYC 332

Query: 245 LDNINGGGIFAI---GEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEV 304
           L + + G   ++      +     T PL++   +   Y+         V +    VG E 
Sbjct: 333 LVDRDSGKSSSLDFNSVQLGGGDATAPLLRNKKIDTFYY---------VGLSGFSVGGEK 392

Query: 305 LNLPTDVFDTDL--KKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLK--LHTVEEQFT 364
           + LP  +FD D     G I+D GT +  L    Y  L          LK    ++    T
Sbjct: 393 VVLPDAIFDVDASGSGGVILDCGTAVTRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDT 452

Query: 365 CFEYDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDI-ASNRWCVGWQNSGSS 409
           C+++        PT+ FHF    SL +    YL  +  S  +C  +  + SS
Sbjct: 453 CYDFSSLSTVKVPTVAFHFTGGKSLDLPAKNYLIPVDDSGTFCFAFAPTSSS 470

[Close]

BLAST of Carg25820 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.0e-28
Identity = 109/396 (27.53%), Postives = 175/396 (44.19%), Query Frame = 0


Query: 28  NVHHKFKGRERSLEAFKAHDVLRR--GRFLSAIDLNLGGNGHPSE--------SGLYFAK 87
           N HH+   R R  +  +   +LRR  G+ + + D     N   S+        SG YF +
Sbjct: 76  NHHHRLHARMRR-DTDRVSAILRRISGKVIPSSDSRYEVNDFGSDIVSGMDQGSGEYFVR 135

Query: 88  IGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYNPSSSSTSNLVTCGQD 147
           IG+G+P +D Y+ +D+GSD++WV C  C  C ++SD      +++P+ S +   V+CG  
Sbjct: 136 IGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSD-----PVFDPAKSGSYTGVSCGSS 195

Query: 148 FCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRVTGNFQTESTNGSIVF 207
            C    +    GC     C Y+V YGDGS T G    + +           ++   ++  
Sbjct: 196 VCDRIENS---GCHSG-GCRYEVMYGDGSYTKGTLALETLTF--------AKTVVRNVAM 255

Query: 208 GCGAQQSGQ-LGATSAALDGILGFGQANSSMISQLASSGKIKRIFAHCL--DNINGGGIF 267
           GCG +  G  +GA      G+LG G  + S + QL  SG+    F +CL     +  G  
Sbjct: 256 GCGHRNRGMFIGAA-----GLLGIGGGSMSFVGQL--SGQTGGAFGYCLVSRGTDSTGSL 315

Query: 268 AIG-EVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVLNLPTDVFD-TD 327
             G E +       PLV+              + Y V +K + VG   + LP  VFD T+
Sbjct: 316 VFGREALPVGASWVPLVRN---------PRAPSFYYVGLKGLGVGGVRIPLPDGVFDLTE 375

Query: 328 L-KKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQF-TCFEYDGNVDDGFP 387
               G ++D+GT +  LP   Y        ++   L   +    F TC++  G V    P
Sbjct: 376 TGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVP 435

Query: 388 TITFHFEDSLSLTVHPHEYLFDI-ASNRWCVGWQNS 406
           T++F+F +   LT+    +L  +  S  +C  +  S
Sbjct: 436 TVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAAS 437

[Close]

BLAST of Carg25820 vs. ExPASy Swiss-Prot
Match: Q9LX20 (Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.7e-28
Identity = 103/350 (29.43%), Postives = 160/350 (45.71%), Query Frame = 0


Query: 73  LYFAKIGLGTPAKDYYVQVDTGSDLLW-----VNCAGCTNCPRKSDLGIELTLYNPSSSS 132
           L++  I +GTP+  + V +DTGS+LLW     V CA  T+    S    +L  YNPSSSS
Sbjct: 99  LHYTWIDIGTPSVSFLVALDTGSNLLWIPCNCVQCAPLTSTYYSSLATKDLNEYNPSSSS 158

Query: 133 TSNLVTCGQDFCTSTYDGPIPGCR-PELLCEYKVAYGDG-SSTTGYFVKDHVVLDRVTGN 192
           TS +  C    C S  D     C  P+  C Y V Y  G +S++G  V+D + L   T N
Sbjct: 159 TSKVFLCSHKLCDSASD-----CESPKEQCPYTVNYLSGNTSSSGLLVEDILHLTYNTNN 218

Query: 193 ---FQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFA 252
                + S    +V GCG +QSG       A DG++G G A  S+ S L+ +G ++  F+
Sbjct: 219 RLMNGSSSVKARVVIGCGKKQSGDY-LDGVAPDGLMGLGPAEISVPSFLSKAGLMRNSFS 278

Query: 253 HCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVL 312
            C D  + G I+  G++     ++TP +Q  + + S         Y V ++A  +GN  L
Sbjct: 279 LCFDEEDSGRIY-FGDMGPSIQQSTPFLQLDNNKYS--------GYIVGVEACCIGNSCL 338

Query: 313 NLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYD 372
              +          T IDSG +  YLP+ IY  +  +I  R         E     + Y+
Sbjct: 339 KQTSFT--------TFIDSGQSFTYLPEEIYRKVALEI-DRHINATSKNFEGVSWEYCYE 398

Query: 373 GNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN--RWCVGWQNSGSSSI 411
            + +   P I   F  + +  +H   ++F  +    ++C+    SG   I
Sbjct: 399 SSAEPKVPAIKLKFSHNNTFVIHKPLFVFQQSQGLVQFCLPISPSGQEGI 424

[Close]

BLAST of Carg25820 vs. ExPASy TrEMBL
Match: A0A6J1G3Q0 (aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111450495 PE=3 SV=1)

HSP 1 Score: 866.7 bits (2238), Expect = 4.4e-248
Identity = 441/496 (88.91%), Postives = 443/496 (89.31%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           M IARLAVVSFLL IS LSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MAIARLAVVSFLLVISLLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVGNE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. ExPASy TrEMBL
Match: A0A6J1IBA4 (aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111471359 PE=3 SV=1)

HSP 1 Score: 857.1 bits (2213), Expect = 3.5e-245
Identity = 436/496 (87.90%), Postives = 442/496 (89.11%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIARLAVVSFLL IS LSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL
Sbjct: 1   MEIARLAVVSFLLVISLLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
           NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL
Sbjct: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD
Sbjct: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNF+TESTNGSIVFGCGAQQS QLGATSAALDGILGFGQANSSMISQLASSGKIKRI
Sbjct: 181 RVTGNFKTESTNGSIVFGCGAQQSVQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ            +AHYNVFMKAIEVG+E
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQ------------QAHYNVFMKAIEVGDE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDL+KGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLRKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVGPHNL SAS+VRVGTISSM
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGPHNLYSASSVRVGTISSM 480

[Close]

BLAST of Carg25820 vs. ExPASy TrEMBL
Match: A0A0A0LJW2 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G099480 PE=3 SV=1)

HSP 1 Score: 783.5 bits (2022), Expect = 4.9e-223
Identity = 395/496 (79.64%), Postives = 419/496 (84.48%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEIAR AVVSF L ISF S+GDCNLV  V HKFKGRERSLEAFKAHD+ RRGRFLSAIDL
Sbjct: 1   MEIARFAVVSFFLVISFFSSGDCNLVLKVQHKFKGRERSLEAFKAHDIQRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
            LGGNGHPSESGLYFAKIGLGTP +DYYVQVDTGSD+LWVNCAGCTNCP+KSDLGIEL+L
Sbjct: 61  QLGGNGHPSESGLYFAKIGLGTPVQDYYVQVDTGSDILWVNCAGCTNCPKKSDLGIELSL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           Y+PSSSSTSN VTC QDFCTSTYDGPIPGC PELLCEY+VAYGDGSST GYFV+DHVVLD
Sbjct: 121 YSPSSSSTSNRVTCNQDFCTSTYDGPIPGCTPELLCEYRVAYGDGSSTAGYFVRDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQT STNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGK+KR+
Sbjct: 181 RVTGNFQTTSTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKVKRV 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEVVQPKVRTTPLV Q            +AHYNVFMKAIEV NE
Sbjct: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVPQ------------QAHYNVFMKAIEVDNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDL+KGTIIDSGTTLAY PDVIYEPLI+KIFARQ+ LKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLRKGTIIDSGTTLAYFPDVIYEPLISKIFARQSTLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPT+TFHFEDSLSLTV+PHEYLFDI SN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTVTFHFEDSLSLTVYPHEYLFDIDSNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVG H+LSSAS++RV  IS +
Sbjct: 421 LVLQNRLVMYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGSHDLSSASSLRVERISVL 480

[Close]

BLAST of Carg25820 vs. ExPASy TrEMBL
Match: A0A1S3B4Q6 (aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103485799 PE=3 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 2.5e-219
Identity = 390/496 (78.63%), Postives = 418/496 (84.27%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDL 60
           MEI R AVVSF L   FLS+ DCNLVF V HKFKGRERSL+AFKAHD+ RRGRFLSAIDL
Sbjct: 1   MEIPRFAVVSFFLV--FLSSVDCNLVFKVQHKFKGRERSLQAFKAHDIHRRGRFLSAIDL 60

Query: 61  NLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTL 120
            LGGNGHPSESGLYFAKIGLGTP +DYYVQVDTGSD+LWVNCAGCTNCP+KSDLGIEL+L
Sbjct: 61  QLGGNGHPSESGLYFAKIGLGTPVQDYYVQVDTGSDILWVNCAGCTNCPKKSDLGIELSL 120

Query: 121 YNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLD 180
           Y+PSSSSTSN VTC QDFCTSTYDGPIPGC P+LLCEY+VAYGDGSST GYFV+DHVVLD
Sbjct: 121 YSPSSSSTSNRVTCNQDFCTSTYDGPIPGCTPDLLCEYRVAYGDGSSTAGYFVRDHVVLD 180

Query: 181 RVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRI 240
           RVTGNFQT STNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGK+KR+
Sbjct: 181 RVTGNFQTTSTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKVKRV 240

Query: 241 FAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNE 300
           FAHCLDNINGGGIFAIGEV+QPKVRTTPLV Q            +AHYNVFMKAIEV NE
Sbjct: 241 FAHCLDNINGGGIFAIGEVLQPKVRTTPLVPQ------------QAHYNVFMKAIEVDNE 300

Query: 301 VLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFE 360
           VLNLPTDVFDTDL+KGTIIDSGTTLAY PDVIYEPLI+KIFARQ+ LKLHTVEEQFTCFE
Sbjct: 301 VLNLPTDVFDTDLRKGTIIDSGTTLAYFPDVIYEPLISKIFARQSTLKLHTVEEQFTCFE 360

Query: 361 YDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG-------------- 420
           YDGNVDDGFPT+TFHFEDSLSLTV+PHEYLFDI SN+WCVGWQNSG              
Sbjct: 361 YDGNVDDGFPTVTFHFEDSLSLTVYPHEYLFDIDSNKWCVGWQNSGAQSRDGKDMILLGD 420

Query: 421 ------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSM 459
                                   SSSIKVRDEHSGAIYTVG H+LSSAS++RV  IS  
Sbjct: 421 LVLQNRLVLYDLENQTIGWTEYNCSSSIKVRDEHSGAIYTVGSHDLSSASSLRVERIS-- 480

[Close]

BLAST of Carg25820 vs. ExPASy TrEMBL
Match: A0A6J1CTW5 (aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC111014180 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 3.2e-214
Identity = 381/498 (76.51%), Postives = 414/498 (83.13%), Query Frame = 0


Query: 1   MEIARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSA--I 60
           MEIAR +VVS L+ IS L++GDCNLVF V HKFKGRE+SL AF+ HD+ RRGRFLS   I
Sbjct: 1   MEIARFSVVSCLVVISLLASGDCNLVFQVEHKFKGREKSLSAFRVHDLHRRGRFLSTSPI 60

Query: 61  DLNLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIEL 120
           DL LGG+G+PSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIEL
Sbjct: 61  DLQLGGSGNPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIEL 120

Query: 121 TLYNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVV 180
           TLYNPSSSSTSN+VTC QDFCTSTYDGPIPGCRP+LLCEYKVAYGDGSST GYFVKDHV+
Sbjct: 121 TLYNPSSSSTSNIVTCNQDFCTSTYDGPIPGCRPDLLCEYKVAYGDGSSTAGYFVKDHVL 180

Query: 181 LDRVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIK 240
           LDRVTGNFQT STNGSI+FGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGK+K
Sbjct: 181 LDRVTGNFQTASTNGSIIFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKVK 240

Query: 241 RIFAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVG 300
           RIFAHCLDNINGGGIFAIGEVVQPKV TT LV+Q            +AHYNV MKAIEVG
Sbjct: 241 RIFAHCLDNINGGGIFAIGEVVQPKVSTTSLVKQ------------QAHYNVVMKAIEVG 300

Query: 301 NEVLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTC 360
           ++VLNLPTDVF+TD +KGTIIDSGTTLAY PDVIY+PLI KIFARQ+GLKLHTVEEQFTC
Sbjct: 301 SDVLNLPTDVFETDFRKGTIIDSGTTLAYFPDVIYDPLITKIFARQSGLKLHTVEEQFTC 360

Query: 361 FEYDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG------------ 420
           F Y GNVDDGFPT+TFHFEDSLSL V+PHEYLFDIA+ +WCVGWQNSG            
Sbjct: 361 FLYHGNVDDGFPTVTFHFEDSLSLIVYPHEYLFDIANGKWCVGWQNSGSQSRDGKDMILL 420

Query: 421 --------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTIS 459
                                     SSSIKVRDE SGA Y+VGPHNLSSAS +R+G IS
Sbjct: 421 GDLVLQNRLVLYDLENQAIGWTEYNCSSSIKVRDESSGATYSVGPHNLSSASAMRIGRIS 480

[Close]

BLAST of Carg25820 vs. TAIR 10
Match: AT1G05840.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 474.6 bits (1220), Expect = 9.3e-134
Identity = 248/488 (50.82%), Postives = 317/488 (64.96%), Query Frame = 0


Query: 5   RLAVVSFLLAISFLSTGDCNL-VFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDLNLG 64
           R  ++ FL A+  +S   CN  VFNV +++   + SL A K HD  R+   L+ IDL LG
Sbjct: 13  RFTLIWFLTALVSVS---CNPGVFNVKYRYPRLQGSLTALKEHDDRRQLTILAGIDLPLG 72

Query: 65  GNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYNP 124
           G G P   GLY+AKIG+GTPAK YYVQVDTGSD++WVNC  C  CPR+S LGIELTLYN 
Sbjct: 73  GTGRPDIPGLYYAKIGIGTPAKSYYVQVDTGSDIMWVNCIQCKQCPRRSTLGIELTLYNI 132

Query: 125 SSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRVT 184
             S +  LV+C  DFC     GP+ GC+  + C Y   YGDGSST GYFVKD V  D V 
Sbjct: 133 DESDSGKLVSCDDDFCYQISGGPLSGCKANMSCPYLEIYGDGSSTAGYFVKDVVQYDSVA 192

Query: 185 GNFQTESTNGSIVFGCGAQQSGQLGATS-AALDGILGFGQANSSMISQLASSGKIKRIFA 244
           G+ +T++ NGS++FGCGA+QSG L +++  ALDGILGFG+ANSSMISQLASSG++K+IFA
Sbjct: 193 GDLKTQTANGSVIFGCGARQSGDLDSSNEEALDGILGFGKANSSMISQLASSGRVKKIFA 252

Query: 245 HCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVL 304
           HCLD  NGGGIFAIG VVQPKV  TPLV              + HYNV M A++VG E L
Sbjct: 253 HCLDGRNGGGIFAIGRVVQPKVNMTPLVPN------------QPHYNVNMTAVQVGQEFL 312

Query: 305 NLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYD 364
            +P D+F    +KG IIDSGTTLAYLP++IYEPL+ KI +++  LK+H V++ + CF+Y 
Sbjct: 313 TIPADLFQPGDRKGAIIDSGTTLAYLPEIIYEPLVKKITSQEPALKVHIVDKDYKCFQYS 372

Query: 365 GNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG---------------- 424
           G VD+GFP +TFHFE+S+ L V+PH+YLF      WC+GWQNS                 
Sbjct: 373 GRVDEGFPNVTFHFENSVFLRVYPHDYLFP-HEGMWCIGWQNSAMQSRDRRNMTLLGDLV 432

Query: 425 ----------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSMLL 453
                                 SSSIKV+DE +G ++ VG H +SSA  + + T   +L 
Sbjct: 433 LSNKLVLYDLENQLIGWTEYNCSSSIKVKDEGTGTVHLVGSHFISSA--LPLDTSMCLLF 482

[Close]

BLAST of Carg25820 vs. TAIR 10
Match: AT3G02740.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 473.8 bits (1218), Expect = 1.6e-133
Identity = 252/474 (53.16%), Postives = 310/474 (65.40%), Query Frame = 0


Query: 16  SFLSTGDCNLVFNVHHKFKG-RERSLEAFKAHDVLRRGRFLSAIDLNLGGNGHPSESGLY 75
           S  +T   NLVF V  KF G R + L A +AHDV R  R LSAID+ LGG+  P   GLY
Sbjct: 26  STAATASENLVFEVRSKFAGKRVKDLGALRAHDVHRHSRLLSAIDIPLGGDSQPESIGLY 85

Query: 76  FAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYNPSSSSTSNLVTC 135
           FAKIGLGTP++D++VQVDTGSD+LWVNCAGC  CPRKSDL +ELT Y+  +SST+  V+C
Sbjct: 86  FAKIGLGTPSRDFHVQVDTGSDILWVNCAGCIRCPRKSDL-VELTPYDVDASSTAKSVSC 145

Query: 136 GQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRVTGNFQTESTNGS 195
             +FC  +Y      C     C+Y + YGDGSST GY VKD V LD VTGN QT STNG+
Sbjct: 146 SDNFC--SYVNQRSECHSGSTCQYVIMYGDGSSTNGYLVKDVVHLDLVTGNRQTGSTNGT 205

Query: 196 IVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFAHCLDNINGGGIF 255
           I+FGCG++QSGQLG + AA+DGI+GFGQ+NSS ISQLAS GK+KR FAHCLDN NGGGIF
Sbjct: 206 IIFGCGSKQSGQLGESQAAVDGIMGFGQSNSSFISQLASQGKVKRSFAHCLDNNNGGGIF 265

Query: 256 AIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVLNLPTDVFDTDLK 315
           AIGEVV PKV+TTP++ +             AHY+V + AIEVGN VL L ++ FD+   
Sbjct: 266 AIGEVVSPKVKTTPMLSK------------SAHYSVNLNAIEVGNSVLELSSNAFDSGDD 325

Query: 316 KGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYDGNVDDGFPTITF 375
           KG IIDSGTTL YLPD +Y PL+ +I A    L LHTV+E FTCF Y   + D FPT+TF
Sbjct: 326 KGVIIDSGTTLVYLPDAVYNPLLNEILASHPELTLHTVQESFTCFHYTDKL-DRFPTVTF 385

Query: 376 HFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG---------------------------- 435
            F+ S+SL V+P EYLF +  + WC GWQN G                            
Sbjct: 386 QFDKSVSLAVYPREYLFQVREDTWCFGWQNGGLQTKGGASLTILGDMALSNKLVVYDIEN 445

Query: 436 ----------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTISSMLLSILLLL 451
                     S  I+V+DE SGAIYTVG HNLS +S++ +    + LL+++ LL
Sbjct: 446 QVIGWTNHNCSGGIQVKDEESGAIYTVGAHNLSWSSSLAI----TKLLTLVSLL 479

[Close]

BLAST of Carg25820 vs. TAIR 10
Match: AT5G36260.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 435.6 bits (1119), Expect = 4.8e-122
Identity = 235/495 (47.47%), Postives = 306/495 (61.82%), Query Frame = 0


Query: 3   IARLAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSAIDLNL 62
           I+R+  V F+L I  +S    N VFNV HKF G+E+ L   K+HD  R  R L+ IDL L
Sbjct: 10  ISRIVAVVFVLVIQVVSG---NFVFNVTHKFAGKEKQLSELKSHDSFRHARMLANIDLPL 69

Query: 63  GGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIELTLYN 122
           GG+      GLYF KI LG+P K+YYVQVDTGSD+LWVNCA C  CP K+DLGI L+LY+
Sbjct: 70  GGDSRADSIGLYFTKIKLGSPPKEYYVQVDTGSDILWVNCAPCPKCPVKTDLGIPLSLYD 129

Query: 123 PSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHVVLDRV 182
             +SSTS  V C  DFC+        G +    C Y V YGDGS++ G F+KD++ L++V
Sbjct: 130 SKTSSTSKNVGCEDDFCSFIMQSETCGAKKP--CSYHVVYGDGSTSDGDFIKDNITLEQV 189

Query: 183 TGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKIKRIFA 242
           TGN +T      +VFGCG  QSGQLG T +A+DGI+GFGQ+N+S+ISQLA+ G  KRIF+
Sbjct: 190 TGNLRTAPLAQEVVFGCGKNQSGQLGQTDSAVDGIMGFGQSNTSIISQLAAGGSTKRIFS 249

Query: 243 HCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVL 302
           HCLDN+NGGGIFA+GEV  P V+TTP+V              + HYNV +K ++V  + +
Sbjct: 250 HCLDNMNGGGIFAVGEVESPVVKTTPIVPN------------QVHYNVILKGMDVDGDPI 309

Query: 303 NLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFTCFEYD 362
           +LP  +  T+   GTIIDSGTTLAYLP  +Y  LI KI A+Q  +KLH V+E F CF + 
Sbjct: 310 DLPPSLASTNGDGGTIIDSGTTLAYLPQNLYNSLIEKITAKQQ-VKLHMVQETFACFSFT 369

Query: 363 GNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG---------------- 422
            N D  FP +  HFEDSL L+V+PH+YLF +  + +C GWQ+ G                
Sbjct: 370 SNTDKAFPVVNLHFEDSLKLSVYPHDYLFSLREDMYCFGWQSGGMTTQDGADVILLGDLV 429

Query: 423 ----------------------SSSIKVRDEHSGAIYTVGPHNL-SSASTVRVGTISSML 459
                                 SSSIKV+D  SGA Y +G  NL S+AS+V  GT+ ++L
Sbjct: 430 LSNKLVVYDLENEVIGWADHNCSSSIKVKD-GSGAAYQLGAENLISAASSVMNGTLVTLL 482

[Close]

BLAST of Carg25820 vs. TAIR 10
Match: AT1G65240.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 402.5 bits (1033), Expect = 4.5e-112
Identity = 213/491 (43.38%), Postives = 297/491 (60.49%), Query Frame = 0


Query: 1   MEIAR---LAVVSFLLAISFLSTGDCNLVFNVHHKFKGRERSLEAFKAHDVLRRGRFLSA 60
           ME+ R   + V  F++ I F S    N VF   HKF G++++LE FK+HD  R  R L++
Sbjct: 1   MELRRKLCIVVAVFVIVIEFAS---ANFVFKAQHKFAGKKKNLEHFKSHDTRRHSRMLAS 60

Query: 61  IDLNLGGNGHPSESGLYFAKIGLGTPAKDYYVQVDTGSDLLWVNCAGCTNCPRKSDLGIE 120
           IDL LGG+      GLYF KI LG+P K+Y+VQVDTGSD+LW+NC  C  CP K++L   
Sbjct: 61  IDLPLGGDSRVDSVGLYFTKIKLGSPPKEYHVQVDTGSDILWINCKPCPKCPTKTNLNFR 120

Query: 121 LTLYNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRPELLCEYKVAYGDGSSTTGYFVKDHV 180
           L+L++ ++SSTS  V C  DFC  ++      C+P L C Y + Y D S++ G F++D +
Sbjct: 121 LSLFDMNASSTSKKVGCDDDFC--SFISQSDSCQPALGCSYHIVYADESTSDGKFIRDML 180

Query: 181 VLDRVTGNFQTESTNGSIVFGCGAQQSGQLGATSAALDGILGFGQANSSMISQLASSGKI 240
            L++VTG+ +T      +VFGCG+ QSGQLG   +A+DG++GFGQ+N+S++SQLA++G  
Sbjct: 181 TLEQVTGDLKTGPLGQEVVFGCGSDQSGQLGNGDSAVDGVMGFGQSNTSVLSQLAATGDA 240

Query: 241 KRIFAHCLDNINGGGIFAIGEVVQPKVRTTPLVQQHSLRLSYFMVHCRAHYNVFMKAIEV 300
           KR+F+HCLDN+ GGGIFA+G V  PKV+TTP+V              + HYNV +  ++V
Sbjct: 241 KRVFSHCLDNVKGGGIFAVGVVDSPKVKTTPMVPN------------QMHYNVMLMGMDV 300

Query: 301 GNEVLNLPTDVFDTDLKKGTIIDSGTTLAYLPDVIYEPLIAKIFARQTGLKLHTVEEQFT 360
               L+LP  +       GTI+DSGTTLAY P V+Y+ LI  I ARQ  +KLH VEE F 
Sbjct: 301 DGTSLDLPRSIVRNG---GTIVDSGTTLAYFPKVLYDSLIETILARQP-VKLHIVEETFQ 360

Query: 361 CFEYDGNVDDGFPTITFHFEDSLSLTVHPHEYLFDIASNRWCVGWQNSG----------- 420
           CF +  NVD+ FP ++F FEDS+ LTV+PH+YLF +    +C GWQ  G           
Sbjct: 361 CFSFSTNVDEAFPPVSFEFEDSVKLTVYPHDYLFTLEEELYCFGWQAGGLTTDERSEVIL 420

Query: 421 ---------------------------SSSIKVRDEHSGAIYTVGPHNLSSASTVRVGTI 451
                                      SSSIK++D  SG +Y+VG  NLSSA  + + T 
Sbjct: 421 LGDLVLSNKLVVYDLDNEVIGWADHNCSSSIKIKD-GSGGVYSVGADNLSSAPRLLMITK 469

[Close]

BLAST of Carg25820 vs. TAIR 10
Match: AT5G22850.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 273.9 bits (699), Expect = 2.4e-73
Identity = 149/388 (38.40%), Postives = 210/388 (54.12%), Query Frame = 0


Query: 37  ERSLEAFKAHDVLRRGRFLSA----IDLNLGGNGHPSESGLYFAKIGLGTPAKDYYVQVD 96
           E  L   KA D  R GR L +    ID  + G   P   GLY+ K+ LGTP +D+YVQVD
Sbjct: 40  EMELSQLKARDEARHGRLLQSLGGVIDFPVDGTFDPFVVGLYYTKLRLGTPPRDFYVQVD 99

Query: 97  TGSDLLWVNCAGCTNCPRKSDLGIELTLYNPSSSSTSNLVTCGQDFCTSTYDGPIPGCRP 156
           TGSD+LWV+CA C  CP+ S L I+L  ++P SS T++ ++C    C+        GC  
Sbjct: 100 TGSDVLWVSCASCNGCPQTSGLQIQLNFFDPGSSVTASPISCSDQRCSWGIQSSDSGCSV 159

Query: 157 E-LLCEYKVAYGDGSSTTGYFVKDHVVLDRVTGNFQTESTNGSIVFGCGAQQSGQLGATS 216
           +  LC Y   YGDGS T+G++V D +  D + G+    ++   +VFGC   Q+G L  + 
Sbjct: 160 QNNLCAYTFQYGDGSGTSGFYVSDVLQFDMIVGSSLVPNSTAPVVFGCSTSQTGDLVKSD 219

Query: 217 AALDGILGFGQANSSMISQLASSGKIKRIFAHCLDNIN-GGGIFAIGEVVQPKVRTTPLV 276
            A+DGI GFGQ   S+ISQLAS G   R+F+HCL   N GGGI  +GE+V+P +  TPLV
Sbjct: 220 RAVDGIFGFGQQGMSVISQLASQGIAPRVFSHCLKGENGGGGILVLGEIVEPNMVFTPLV 279

Query: 277 QQHSLRLSYFMVHCRAHYNVFMKAIEVGNEVLNLPTDVFDTDLKKGTIIDSGTTLAYLPD 336
                         + HYNV + +I V  + L +   VF T   +GTIID+GTTLAYL +
Sbjct: 280 PS------------QPHYNVNLLSISVNGQALPINPSVFSTSNGQGTIIDTGTTLAYLSE 339

Query: 337 VIYEPLIAKIFARQTGLKLHTVEEQFTCFEYDGNVDDGFPTITFHFEDSLSLTVHPHEYL 396
             Y P +  I    +      V +   C+    +V D FP ++ +F    S+ ++P +YL
Sbjct: 340 AAYVPFVEAITNAVSQSVRPVVSKGNQCYVITTSVGDIFPPVSLNFAGGASMFLNPQDYL 399

Query: 397 FDIASNR------WCVGWQNSGSSSIKV 413
             I  N       WC+G+Q   +  I +
Sbjct: 400 --IQQNNVGGTAVWCIGFQRIQNQGITI 413

The following BLAST results are available for this feature:

BLAST of Carg25820 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029946.1	1.5e-266	100.00	Aspartic proteinase-like protein 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6598979.1	1.3e-249	89.52	Aspartic proteinase-like protein 2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022946441.1	9.0e-248	88.91	aspartic proteinase-like protein 2 [Cucurbita moschata]	[more]
XP_023547349.1	2.0e-247	88.71	aspartic proteinase-like protein 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022972843.1	7.1e-245	87.90	aspartic proteinase-like protein 2 [Cucurbita maxima]	[more]

BLAST of Carg25820 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4V3D2	6.7e-121	47.47	Aspartic proteinase 36 OS=Arabidopsis thaliana OX=3702 GN=A36 PE=1 SV=1	[more]
Q9S9K4	6.3e-111	43.38	Aspartic proteinase 39 OS=Arabidopsis thaliana OX=3702 GN=A39 PE=1 SV=2	[more]
Q9LS40	2.5e-30	29.26	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LHE3	1.0e-28	27.53	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LX20	6.7e-28	29.43	Aspartic proteinase-like protein 1 OS=Arabidopsis thaliana OX=3702 GN=At5g10080 ...	[more]

BLAST of Carg25820 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G3Q0	4.4e-248	88.91	aspartic proteinase-like protein 2 OS=Cucurbita moschata OX=3662 GN=LOC111450495...	[more]
A0A6J1IBA4	3.5e-245	87.90	aspartic proteinase-like protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111471359 P...	[more]
A0A0A0LJW2	4.9e-223	79.64	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G09948...	[more]
A0A1S3B4Q6	2.5e-219	78.63	aspartic proteinase-like protein 2 OS=Cucumis melo OX=3656 GN=LOC103485799 PE=3 ...	[more]
A0A6J1CTW5	3.2e-214	76.51	aspartic proteinase-like protein 2 OS=Momordica charantia OX=3673 GN=LOC11101418...	[more]

BLAST of Carg25820 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05840.1	9.3e-134	50.82	Eukaryotic aspartyl protease family protein	[more]
AT3G02740.1	1.6e-133	53.16	Eukaryotic aspartyl protease family protein	[more]
AT5G36260.1	4.8e-122	47.47	Eukaryotic aspartyl protease family protein	[more]
AT1G65240.1	4.5e-112	43.38	Eukaryotic aspartyl protease family protein	[more]
AT5G22850.1	2.4e-73	38.40	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 80..100 score: 48.68 coord: 317..328 score: 42.62
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 9..411
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 64..257 e-value: 3.2E-50 score: 172.8
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 258..423 e-value: 1.1E-29 score: 105.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 69..410
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 74..257 e-value: 1.1E-40 score: 139.7
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 287..407 e-value: 3.7E-16 score: 59.3
None	No IPR available	PANTHER	PTHR13683:SF685	EUKARYOTIC ASPARTYL PROTEASE FAMILY PROTEIN	coord: 9..411
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 74..447 score: 35.208248
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 74..409 e-value: 6.06481E-50 score: 168.981

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25820-RA	Carg25820-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004190	aspartic-type endopeptidase activity