Carg25814 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25814
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionRING/FYVE/PHD zinc finger superfamily protein
LocationCarg_Chr05: 6026369 .. 6030018 (+)
RNA-Seq ExpressionCarg25814
SyntenyCarg25814
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTCCGCACCTAACGCGTTTTCTCTTCCTCTTCCACCTTTCTTTTTCCTTTTCTCCTTCATTTTTTGTCTTCTCAATTCACTCTATTCCTCTTCTTTCTAGTTTCATCCCTGCAATTCTTACCATTCGCCTCTGTAGGCTTTCTTTCTTTTCTTGAATTGATCGCCTGAGTTGGGTTTTTGGTGATATAGGGGTTTTGAGGACAATGAGCGATCATCTTGTCCTGTATGTTGACCATTTGATTCCACCAGTTGCTCCAGTTCAGCCTGTGTCCGACCCGGTTGACCCGGTTCCTTCCGCACCGCGTGAGGGTGTTTCTGACTCGGCTGCGGCTATTCCTTCCTGTTCTACTGCCACTGCGGATGATCGGACGGTGGAGCAGGATGCACCCAATGAGGAGGACCCTCTGATTCAGGCGGCGGAGTGTCGCATTTGTCAGGAAGAAGACACTCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGGTATTGACGCCTCACCCTTTTCTGTTGCTTCTTAAATTTTTACGTGAATACACAGGGGACACCCTTTTACCCTAGTTTGTTGATTTGACTCTGTTTTCTTTCATGGCGTTGGATTTATGAATGCTGTTTTTGGAAACTTCGGATATAGTTCTTTCATACTGGTTCATTTGGGTTTAGTAACTTTCTTCTTTTTACTGTCTTTGGAATGGGTTCAAGTCTATGTTTATTGCTTTGATTTTTGTTTTTTTTTGGTCAATTTTTGTGTTGCTTCCAATGTGGTTTAGTTTTGGCGGAAGCAAGCTGGAAAAGGACATAAAAGAACAAGTTTATCTAGTATTTTCCAAAACTGTGATGGATGAGAGTTTGCCTGAATTCTACAGTGTCTTGTTTAGAATTGACATGCTCATATCTTGAGAGGATTTATATCGTTGATGTGGGGAGGATTTTTGTGTTGTTTGGTGGTCATTTGTTTTACTCTTTGTTTGGTCAATTGAATATTTCTTTATAAAATTTTCCCCAATGCTTCGTTTGTGACGTTTAATATCATCTCTCGTCTAATAGTTTCGTCACAAGAGTAACGCTTTTGCCTAAACTCTTCTGTTTGCATAAATTTCTATGATCGATGGGCAATTTTATGAGCTGTATGTGGGGGTATGTGCTGCGTCTGAGTTATAAAGTGGCTTGATAAAATTTGACACGAATTATGGTTATCATTCATGATGTCATGGTAGAATTATAGTGATCCATGAACTCTATTTTTTTTTTCTCTCCAGAATGTAACTAAGTTTGGTTATGCCCTTAGAATTGACCAATACGATCTAGTATAGGAAAATTTATCGGGCGTGCTTGTTTATTAATCTTTTGGCACGTGTCTGATGAATGTTTGTGTTCAGTATGCTCATAGGAAGTGCGTTCAACATTGGTGCAATGAGAAAGGAGACATAACTTGTGAGATATGTCATCAGGTATGCATGATGTTTTGATTTGATATTCGGCTATTATAGTATTATTTTGTAAAAAAATTTCAAATAAGTTGCTAATATTATCTTTATTCTTTAGCTTCCGTGCAAGTTTTCCTCCTAGTATATTTTTGTTGAACTCATTACTCTTTGAATTTTGAAAAAAGGCATGCTTTACACCGGTAGTTCAAAATTTTCATTATTAGTGATTTAAAATGTGCATAAAAAATGAACGCGTACGAATGAGAGGGAGAAAACCATATGATTCGCAATTTGCTAGGGTTGCTGTTGTAGGAGAAAACTGTAGGACTCGAGAACGAGACTTTGCTACTTTAGAAACCTCATGGATGATTGGAGACTCTTGCCCGTTTACTTCTCTTCGTACTTGTCTGATTGTCTCTACTCTCGACCAGTCTCTTTTGTCTATCCTCTCCGAGCCATACCTTTTGTTCGTCTTTTCCTTTGACACATCCATCTCTGAGAAAAGCATAACAGTGGTCAGTGGTTTACTATAGCGCACACAATGATTTGGATCTTTTTGCAATGGGCTGGATTCCTTTTCGGATAGTTTGAATCTGACATCATAGAAGATTGTTCCAAGTCATTGAGGAGAAGATGGGCAGCATGTGAATTGGTGGATTCTTCCCTTCCTCATGCTCAACCAAAAGTAATGTGAATGCTACTTCGAATATGCTGTCTGTCAGAGATGGTAGAGGTAAGGAGTGTCTACCATGAAGATGCAGGTTCATTAGTGTTGAAGTGTGATGATGGATGAGGCATTGATGTTTCCTCAGACTTATGTCTTAACACATATCTCTCGCTGTTGGAGCTTCGTTTGTTAAATGAAAAGTTGGTTCGATGTTTTAGTCTTTCCAAATTTTGAGCGTGGGGCTCTCGATTCAGGACATTGGACTCTTTTTTCTGCTCTGTCTGTGATTCTTTCCCCTTTATCTTTACGTGTTTCATGTTATGTTGAATGGTATCAATATCATGTGATTTTCATTTGCAGTTTCTTTTCTGATGATGTTTTTCTTGTTTCAATTCCTCATTGATTGATATTCCGGTACGCTTTATTCAATTGCCAGTCTTATCGACCTGGATATACTGCACCCCCTCGTTCTGAAGAAACTGTGATTGATATGGAGTAAGTTCAACCTTCTTGTTATTTGCAATTTGCATGTGAATGACTTTATCAATAATCATGCATCTGAACACCCACAACCGTTTTATTTTCATGTAATTTGTACACGCATTTGAAATATTGACCTGGATGTGGATATGCACAGGGGACGATGGACTATTGCTGGTAACCCGTTAGACCTTAGAGATACTCGGGTACTGGCCATTGCGGAGGCAGAACGTAATTTCTTGGAAGCCGAATATGATGATTATACTGCTTCAAATGATAGTGGAGCTGCATTTTGCCGATCAGCTGCCCTTATTGTAAGTTTTCTTATGTTAGTTCATAGAACTCTTGTATTTTCCATGAAACTCTGATGATTGATGAAGTATTTGATTTTACTGGCTAGCTATTAGCCCTTCTCCTTCTGCGACACGCATTGACCATAACTGATCCAGACGGTGATGATGACGCATCTGCCTTTTTCTCTGTAAGCGAGATGGGAATTCGTTTTCTTAAATTTGAAAGAACAATAGTATAATCGGAAGGATATTTATAATCTGGATGTACCTTTGACAGATTTTCTTGCTTCGTGCTGCTGGTTTTCTTCTACCATGTTACATCATGGCTTGGGCCATCAGTATCCTGCAACGTCGTCGACAAAGACAGGTTGGTTAGCGATTAAGTTCTTCGGGTGTATTTTTGAAAATTCTGTCTTAACAACTCTAGGTGTTGCCATCTCTTAAATAAGTAGATATTTTGGCATATATTGTAGAAAGCAAGGATGTCTGTGTAGGGGGTAGGCAAAATTGTTGGTCTCTAAGAATGTTGTTGACACTTAAAGTTTGGTGGTTGTGAACCAACAGGAGGCGGCAGCACTGGCTGCAACACAAGTGGCGTTTGTGCTACAACATGGACAGCATAGAGGTTTACAATTTGCAATTGCATCAGCAGGACCGCCCCTCACCCCTCACCCTCACCCTCACCCTCACCCACACCCACACCCTCATCCTCACCCTCACCCCCACCCACACCCACACCCTCCCCCTCACCCACACCCTCACCCTGCTGTTTGA

mRNA sequence

ATTCCGCACCTAACGCGTTTTCTCTTCCTCTTCCACCTTTCTTTTTCCTTTTCTCCTTCATTTTTTGTCTTCTCAATTCACTCTATTCCTCTTCTTTCTAGTTTCATCCCTGCAATTCTTACCATTCGCCTCTGTAGGCTTTCTTTCTTTTCTTGAATTGATCGCCTGAGTTGGGTTTTTGGTGATATAGGGGTTTTGAGGACAATGAGCGATCATCTTGTCCTGTATGTTGACCATTTGATTCCACCAGTTGCTCCAGTTCAGCCTGTGTCCGACCCGGTTGACCCGGTTCCTTCCGCACCGCGTGAGGGTGTTTCTGACTCGGCTGCGGCTATTCCTTCCTGTTCTACTGCCACTGCGGATGATCGGACGGTGGAGCAGGATGCACCCAATGAGGAGGACCCTCTGATTCAGGCGGCGGAGTGTCGCATTTGTCAGGAAGAAGACACTCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGTATGCTCATAGGAAGTGCGTTCAACATTGGTGCAATGAGAAAGGAGACATAACTTGTGAGATATGTCATCAGTCTCTTTTGTCTATCCTCTCCGAGCCATACCTTTTGTTCGTCTTTTCCTTTGACACATCCATCTCTGAGAAAAGCATAACAGTGTTTGAATCTGACATCATAGAAGATTGTTCCAAGTCATTGAGGAGAAGATGGGCAGCATGTGAATTGGTGGATTCTTCCCTTCCTCATGCTCAACCAAAATCTTATCGACCTGGATATACTGCACCCCCTCGTTCTGAAGAAACTGTGATTGATATGGAGGGACGATGGACTATTGCTGGTAACCCGTTAGACCTTAGAGATACTCGGGTACTGGCCATTGCGGAGGCAGAACGTAATTTCTTGGAAGCCGAATATGATGATTATACTGCTTCAAATGATAGTGGAGCTGCATTTTGCCGATCAGCTGCCCTTATTCTATTAGCCCTTCTCCTTCTGCGACACGCATTGACCATAACTGATCCAGACGGTGATGATGACGCATCTGCCTTTTTCTCTATTTTCTTGCTTCGTGCTGCTGGTTTTCTTCTACCATGTTACATCATGGCTTGGGCCATCAGTATCCTGCAACGTCGTCGACAAAGACAGGAGGCGGCAGCACTGGCTGCAACACAAGTGGCGTTTGTGCTACAACATGGACAGCATAGAGGTTTACAATTTGCAATTGCATCAGCAGGACCGCCCCTCACCCCTCACCCTCACCCTCACCCTCACCCACACCCACACCCTCATCCTCACCCTCACCCCCACCCACACCCACACCCTCCCCCTCACCCACACCCTCACCCTGCTGTTTGA

Coding sequence (CDS)

ATGAGCGATCATCTTGTCCTGTATGTTGACCATTTGATTCCACCAGTTGCTCCAGTTCAGCCTGTGTCCGACCCGGTTGACCCGGTTCCTTCCGCACCGCGTGAGGGTGTTTCTGACTCGGCTGCGGCTATTCCTTCCTGTTCTACTGCCACTGCGGATGATCGGACGGTGGAGCAGGATGCACCCAATGAGGAGGACCCTCTGATTCAGGCGGCGGAGTGTCGCATTTGTCAGGAAGAAGACACTCTCAATAATTTAGAAACTCCCTGCGCATGTAGTGGTAGTCTTAAGTATGCTCATAGGAAGTGCGTTCAACATTGGTGCAATGAGAAAGGAGACATAACTTGTGAGATATGTCATCAGTCTCTTTTGTCTATCCTCTCCGAGCCATACCTTTTGTTCGTCTTTTCCTTTGACACATCCATCTCTGAGAAAAGCATAACAGTGTTTGAATCTGACATCATAGAAGATTGTTCCAAGTCATTGAGGAGAAGATGGGCAGCATGTGAATTGGTGGATTCTTCCCTTCCTCATGCTCAACCAAAATCTTATCGACCTGGATATACTGCACCCCCTCGTTCTGAAGAAACTGTGATTGATATGGAGGGACGATGGACTATTGCTGGTAACCCGTTAGACCTTAGAGATACTCGGGTACTGGCCATTGCGGAGGCAGAACGTAATTTCTTGGAAGCCGAATATGATGATTATACTGCTTCAAATGATAGTGGAGCTGCATTTTGCCGATCAGCTGCCCTTATTCTATTAGCCCTTCTCCTTCTGCGACACGCATTGACCATAACTGATCCAGACGGTGATGATGACGCATCTGCCTTTTTCTCTATTTTCTTGCTTCGTGCTGCTGGTTTTCTTCTACCATGTTACATCATGGCTTGGGCCATCAGTATCCTGCAACGTCGTCGACAAAGACAGGAGGCGGCAGCACTGGCTGCAACACAAGTGGCGTTTGTGCTACAACATGGACAGCATAGAGGTTTACAATTTGCAATTGCATCAGCAGGACCGCCCCTCACCCCTCACCCTCACCCTCACCCTCACCCACACCCACACCCTCATCCTCACCCTCACCCCCACCCACACCCACACCCTCCCCCTCACCCACACCCTCACCCTGCTGTTTGA

Protein sequence

MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQPKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTASNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHPHPHPHPHPHPPPHPHPHPAV
Homology
BLAST of Carg25814 vs. NCBI nr
Match: KAG7029952.1 (E3 ubiquitin-protein ligase MARCH3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 743.4 bits (1918), Expect = 9.5e-211
Identity = 380/380 (100.00%), Postives = 380/380 (100.00%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ
Sbjct: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
           PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS
Sbjct: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360
           ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP
Sbjct: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360

Query: 361 HPHPHPHPHPPPHPHPHPAV 381
           HPHPHPHPHPPPHPHPHPAV
Sbjct: 361 HPHPHPHPHPPPHPHPHPAV 380

BLAST of Carg25814 vs. NCBI nr
Match: XP_022946694.1 (uncharacterized protein LOC111450687 [Cucurbita moschata])

HSP 1 Score: 587.0 bits (1512), Expect = 1.1e-163
Identity = 313/378 (82.80%), Postives = 315/378 (83.33%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360
           ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP
Sbjct: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 317

Query: 361 HPHPHPHPHPPPHPHPHP 379
           HPHPHPHPHP PHPHPHP
Sbjct: 361 HPHPHPHPHPHPHPHPHP 317

BLAST of Carg25814 vs. NCBI nr
Match: XP_023547303.1 (uncharacterized protein LOC111806165 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 580.9 bits (1496), Expect = 8.2e-162
Identity = 310/378 (82.01%), Postives = 313/378 (82.80%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDP+PSAPREGVSDSAAAIPS STATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPIPSAPREGVSDSAAAIPSSSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360
           ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPL PHPHPHPHPHPHPHP
Sbjct: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLAPHPHPHPHPHPHPHP 317

Query: 361 HPHPHPHPHPPPHPHPHP 379
           HPHPHPHPHP PHPHPHP
Sbjct: 361 HPHPHPHPHPHPHPHPHP 317

BLAST of Carg25814 vs. NCBI nr
Match: KAG6598983.1 (putative apyrase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 577.8 bits (1488), Expect = 6.9e-161
Identity = 305/370 (82.43%), Postives = 307/370 (82.97%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTAT DDRTVEQD
Sbjct: 562 MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATVDDRTVEQD 621

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 622 APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 681

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 682 Q----------------------------------------------------------- 741

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 742 --SYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 801

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 802 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 861

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360
           ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP
Sbjct: 862 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 870

Query: 361 HPHPHPHPHP 371
           HPHPHPHPHP
Sbjct: 922 HPHPHPHPHP 870

BLAST of Carg25814 vs. NCBI nr
Match: XP_022972852.1 (uncharacterized protein LOC111471364 isoform X1 [Cucurbita maxima])

HSP 1 Score: 552.4 bits (1422), Expect = 3.1e-153
Identity = 295/365 (80.82%), Postives = 300/365 (82.19%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPS STATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGY+AP RSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDG-DDDASAFFSIFLLRAAGFLLPCYIMAW 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDG DDDA+AFFSIFLLRAAGFLLPCYIMAW
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMAW 300

Query: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 360
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH
Sbjct: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 304

Query: 361 PHPHP 365
           PHP+P
Sbjct: 361 PHPNP 304

BLAST of Carg25814 vs. ExPASy TrEMBL
Match: A0A6J1G4K7 (uncharacterized protein LOC111450687 OS=Cucurbita moschata OX=3662 GN=LOC111450687 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.5e-164
Identity = 313/378 (82.80%), Postives = 315/378 (83.33%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 360
           ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP
Sbjct: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPHP 317

Query: 361 HPHPHPHPHPPPHPHPHP 379
           HPHPHPHPHP PHPHPHP
Sbjct: 361 HPHPHPHPHPHPHPHPHP 317

BLAST of Carg25814 vs. ExPASy TrEMBL
Match: A0A6J1I5Y1 (uncharacterized protein LOC111471364 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471364 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.5e-153
Identity = 295/365 (80.82%), Postives = 300/365 (82.19%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPS STATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGY+AP RSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDG-DDDASAFFSIFLLRAAGFLLPCYIMAW 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDG DDDA+AFFSIFLLRAAGFLLPCYIMAW
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMAW 300

Query: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 360
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH
Sbjct: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 304

Query: 361 PHPHP 365
           PHP+P
Sbjct: 361 PHPNP 304

BLAST of Carg25814 vs. ExPASy TrEMBL
Match: A0A6J1IBB6 (uncharacterized protein LOC111471364 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471364 PE=4 SV=1)

HSP 1 Score: 549.3 bits (1414), Expect = 1.3e-152
Identity = 293/363 (80.72%), Postives = 298/363 (82.09%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPS STATADDRTVEQD
Sbjct: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSSSTATADDRTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH
Sbjct: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
             SY+PGY+AP RSEETVIDMEGRWTIAGNPLDLRDTR+LAIAEAERNFLEAEYDDY AS
Sbjct: 181 --SYQPGYSAPLRSEETVIDMEGRWTIAGNPLDLRDTRLLAIAEAERNFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDG-DDDASAFFSIFLLRAAGFLLPCYIMAW 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDG DDDA+AFFSIFLLRAAGFLLPCYIMAW
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDDAAAFFSIFLLRAAGFLLPCYIMAW 300

Query: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 360
           AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH
Sbjct: 301 AISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHPHPHPH 302

Query: 361 PHP 363
           P+P
Sbjct: 361 PNP 302

BLAST of Carg25814 vs. ExPASy TrEMBL
Match: A0A6J1CTP2 (uncharacterized protein LOC111014120 OS=Momordica charantia OX=3673 GN=LOC111014120 PE=4 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 1.2e-142
Identity = 272/356 (76.40%), Postives = 284/356 (79.78%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTAT--ADDRTVE 60
           MSDHLVLYVDHLI PVAPV+PVS+P DPVPSAPRE V DSAAA PSCSTAT  ADDRTV+
Sbjct: 1   MSDHLVLYVDHLIRPVAPVEPVSEPADPVPSAPREAVVDSAAAGPSCSTATAVADDRTVK 60

Query: 61  QDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120
           Q APNEE+PLIQAAECRICQEED+LNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI
Sbjct: 61  QHAPNEEEPLIQAAECRICQEEDSLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEI 120

Query: 121 CHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPH 180
           CHQ                                                         
Sbjct: 121 CHQ--------------------------------------------------------- 180

Query: 181 AQPKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYT 240
               SY+PGYTAPPR+EET IDMEGRWTI+GNPLDLRDTR+LAIAEAER+FLEAEYDDY 
Sbjct: 181 ----SYQPGYTAPPRTEETAIDMEGRWTISGNPLDLRDTRLLAIAEAERHFLEAEYDDYA 240

Query: 241 ASNDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMA 300
            SNDSGAAFCRSAALILLALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIMA
Sbjct: 241 TSNDSGAAFCRSAALILLALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMA 295

Query: 301 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASAGPPLTPHPHPHPHP 355
           WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS+GPPLTPHPHPHPHP
Sbjct: 301 WAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIASSGPPLTPHPHPHPHP 295

BLAST of Carg25814 vs. ExPASy TrEMBL
Match: A0A6J1G9V2 (uncharacterized protein LOC111452273 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452273 PE=4 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 9.5e-140
Identity = 271/360 (75.28%), Postives = 281/360 (78.06%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           M DHLVLYVDHLI PVAPVQ V++PVDP+PSAPREGV DSAAA PSCSTAT D  TVEQD
Sbjct: 1   MGDHLVLYVDHLIRPVAPVQSVAEPVDPIPSAPREGVVDSAAAGPSCSTATVDHPTVEQD 60

Query: 61  APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCEICH 120
           APNEE+PLIQ AECRICQEED +NNLETPCACSGSLKYAHRKCVQHWCNEKGDI CEICH
Sbjct: 61  APNEEEPLIQVAECRICQEEDGINNLETPCACSGSLKYAHRKCVQHWCNEKGDIICEICH 120

Query: 121 QSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLPHAQ 180
           Q                                                           
Sbjct: 121 Q----------------------------------------------------------- 180

Query: 181 PKSYRPGYTAPPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYDDYTAS 240
              Y+PGYTAP RSEET IDMEGRWTIAGNPLDLRDTR+LAIAEAER+FLEAEYDDY AS
Sbjct: 181 --FYQPGYTAPSRSEETAIDMEGRWTIAGNPLDLRDTRLLAIAEAERHFLEAEYDDYAAS 240

Query: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGDDDASAFFSIFLLRAAGFLLPCYIMAWA 300
           NDSGAAFCRSAALILLALLLLRHALTITDPDG+DDASAFFSIFLLRAAGFLLPCYIMAWA
Sbjct: 241 NDSGAAFCRSAALILLALLLLRHALTITDPDGEDDASAFFSIFLLRAAGFLLPCYIMAWA 299

Query: 301 ISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIAS-AGPPLTPHPHPHPHPHPHPH 360
           ISILQRRRQRQEAAA+AATQVAFVLQHGQHRGLQFAIAS AG PLTPHPHP PHPHPHPH
Sbjct: 301 ISILQRRRQRQEAAAVAATQVAFVLQHGQHRGLQFAIASAAGAPLTPHPHPLPHPHPHPH 299

BLAST of Carg25814 vs. TAIR 10
Match: AT3G47550.3 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.3e-76
Identity = 172/358 (48.04%), Postives = 213/358 (59.50%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           M+DHL L  D LI         S+ +D    +   G S         ++++ ++  V ++
Sbjct: 1   MADHLSLCTDRLI--------TSESLDSEKDSDSSGESSYRPQGTDLASSSVNETEVPRE 60

Query: 61  ---APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 120
                +EE+PL+Q+ ECRICQEED+  NLE PCAC+GSLKYAHRKCVQ WCNEKGDITCE
Sbjct: 61  YYAVADEEEPLLQSVECRICQEEDSTKNLEAPCACNGSLKYAHRKCVQRWCNEKGDITCE 120

Query: 121 ICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLP 180
           ICHQ                                                        
Sbjct: 121 ICHQ-------------------------------------------------------- 180

Query: 181 HAQPKSYRPGYTA--PPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYD 240
                 Y+ GYTA  PP  +ET+I +   W   G PLDL D R+LA+A AER+FLEA+YD
Sbjct: 181 -----PYQHGYTAPPPPPPDETIIHIGDDWE-NGVPLDLTDPRILAMAAAERHFLEADYD 240

Query: 241 DYTASNDSGAAFCRSAALILLALLLLRHALTI-TDPDGDDDASAFFSIFLLRAAGFLLPC 300
           +Y+ +N SGAAFCRSAALIL+ALLLLR AL + T+PD +DD +AFFS+FLLRAAGFLLPC
Sbjct: 241 EYSENNSSGAAFCRSAALILMALLLLRDALNLTTNPDDEDDPTAFFSLFLLRAAGFLLPC 287

Query: 301 YIMAWAISILQRRRQRQEAAALAATQVAFVLQHG--QHRGLQFAIASAGPPLTPHPHP 351
           YIMAWAI ILQRRRQRQEAAALAA +V F++  G  Q RGL FA+A   PP++  P P
Sbjct: 301 YIMAWAIGILQRRRQRQEAAALAAAEVTFMIHGGGPQRRGLHFAVAPQ-PPISNVPTP 287

BLAST of Carg25814 vs. TAIR 10
Match: AT3G47550.6 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 282.0 bits (720), Expect = 7.3e-76
Identity = 172/358 (48.04%), Postives = 213/358 (59.50%), Query Frame = 0

Query: 1   MSDHLVLYVDHLIPPVAPVQPVSDPVDPVPSAPREGVSDSAAAIPSCSTATADDRTVEQD 60
           M+DHL L  D LI         S+ +D    +   G S         ++++ ++  V ++
Sbjct: 1   MADHLSLCTDRLI--------TSESLDSEKDSDSSGESSYRPQGTDLASSSVNETEVPRE 60

Query: 61  ---APNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 120
                +EE+PL+Q+ ECRICQEED+  NLE PCAC+GSLKYAHRKCVQ WCNEKGDITCE
Sbjct: 61  YYAVADEEEPLLQSVECRICQEEDSTKNLEAPCACNGSLKYAHRKCVQRWCNEKGDITCE 120

Query: 121 ICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLP 180
           ICHQ                                                        
Sbjct: 121 ICHQ-------------------------------------------------------- 180

Query: 181 HAQPKSYRPGYTA--PPRSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEYD 240
                 Y+ GYTA  PP  +ET+I +   W   G PLDL D R+LA+A AER+FLEA+YD
Sbjct: 181 -----PYQHGYTAPPPPPPDETIIHIGDDWE-NGVPLDLTDPRILAMAAAERHFLEADYD 240

Query: 241 DYTASNDSGAAFCRSAALILLALLLLRHALTI-TDPDGDDDASAFFSIFLLRAAGFLLPC 300
           +Y+ +N SGAAFCRSAALIL+ALLLLR AL + T+PD +DD +AFFS+FLLRAAGFLLPC
Sbjct: 241 EYSENNSSGAAFCRSAALILMALLLLRDALNLTTNPDDEDDPTAFFSLFLLRAAGFLLPC 287

Query: 301 YIMAWAISILQRRRQRQEAAALAATQVAFVLQHG--QHRGLQFAIASAGPPLTPHPHP 351
           YIMAWAI ILQRRRQRQEAAALAA +V F++  G  Q RGL FA+A   PP++  P P
Sbjct: 301 YIMAWAIGILQRRRQRQEAAALAAAEVTFMIHGGGPQRRGLHFAVAPQ-PPISNVPTP 287

BLAST of Carg25814 vs. TAIR 10
Match: AT2G02960.2 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.0e-74
Identity = 158/286 (55.24%), Postives = 183/286 (63.99%), Query Frame = 0

Query: 58  EQDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 117
           + D  +E +PLI + ECRIC +E  + NLE+PCACSGSLKYAHRKCVQ WCNEKG+I CE
Sbjct: 27  DDDHDDENEPLIVSGECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICE 86

Query: 118 ICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLP 177
           ICHQ                                                        
Sbjct: 87  ICHQ-------------------------------------------------------- 146

Query: 178 HAQPKSYRPGYTAPP---RSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEY 237
                 Y+PGYTAPP   + EET ID+ G WTI+G  LD+ D R+LAIAEAER +LE+EY
Sbjct: 147 -----PYQPGYTAPPPPLQPEETTIDIGGGWTISG--LDVHDPRLLAIAEAERRYLESEY 206

Query: 238 DDYTASNDSGAAFCRSAALILLALLLLRHALTIT-DPDG-DDDASAFFSIFLLRAAGFLL 297
            +YTAS+ SGAAFCRSAALIL+ALLLLRHALTIT D DG +DD S+  S+ LLRAAGFLL
Sbjct: 207 VEYTASSASGAAFCRSAALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLL 248

Query: 298 PCYIMAWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIA 339
           PCYIMAWAISILQRRRQRQEAAAL ATQ A VLQ GQ R + F ++
Sbjct: 267 PCYIMAWAISILQRRRQRQEAAAL-ATQFALVLQSGQPRTVHFTVS 248

BLAST of Carg25814 vs. TAIR 10
Match: AT2G02960.4 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.0e-74
Identity = 158/286 (55.24%), Postives = 183/286 (63.99%), Query Frame = 0

Query: 58  EQDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 117
           + D  +E +PLI + ECRIC +E  + NLE+PCACSGSLKYAHRKCVQ WCNEKG+I CE
Sbjct: 27  DDDHDDENEPLIVSGECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICE 86

Query: 118 ICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLP 177
           ICHQ                                                        
Sbjct: 87  ICHQ-------------------------------------------------------- 146

Query: 178 HAQPKSYRPGYTAPP---RSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEY 237
                 Y+PGYTAPP   + EET ID+ G WTI+G  LD+ D R+LAIAEAER +LE+EY
Sbjct: 147 -----PYQPGYTAPPPPLQPEETTIDIGGGWTISG--LDVHDPRLLAIAEAERRYLESEY 206

Query: 238 DDYTASNDSGAAFCRSAALILLALLLLRHALTIT-DPDG-DDDASAFFSIFLLRAAGFLL 297
            +YTAS+ SGAAFCRSAALIL+ALLLLRHALTIT D DG +DD S+  S+ LLRAAGFLL
Sbjct: 207 VEYTASSASGAAFCRSAALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLL 248

Query: 298 PCYIMAWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIA 339
           PCYIMAWAISILQRRRQRQEAAAL ATQ A VLQ GQ R + F ++
Sbjct: 267 PCYIMAWAISILQRRRQRQEAAAL-ATQFALVLQSGQPRTVHFTVS 248

BLAST of Carg25814 vs. TAIR 10
Match: AT2G02960.1 (RING/FYVE/PHD zinc finger superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 4.0e-74
Identity = 158/286 (55.24%), Postives = 183/286 (63.99%), Query Frame = 0

Query: 58  EQDAPNEEDPLIQAAECRICQEEDTLNNLETPCACSGSLKYAHRKCVQHWCNEKGDITCE 117
           + D  +E +PLI + ECRIC +E  + NLE+PCACSGSLKYAHRKCVQ WCNEKG+I CE
Sbjct: 27  DDDHDDENEPLIVSGECRICSDESPVENLESPCACSGSLKYAHRKCVQRWCNEKGNIICE 86

Query: 118 ICHQSLLSILSEPYLLFVFSFDTSISEKSITVFESDIIEDCSKSLRRRWAACELVDSSLP 177
           ICHQ                                                        
Sbjct: 87  ICHQ-------------------------------------------------------- 146

Query: 178 HAQPKSYRPGYTAPP---RSEETVIDMEGRWTIAGNPLDLRDTRVLAIAEAERNFLEAEY 237
                 Y+PGYTAPP   + EET ID+ G WTI+G  LD+ D R+LAIAEAER +LE+EY
Sbjct: 147 -----PYQPGYTAPPPPLQPEETTIDIGGGWTISG--LDVHDPRLLAIAEAERRYLESEY 206

Query: 238 DDYTASNDSGAAFCRSAALILLALLLLRHALTIT-DPDG-DDDASAFFSIFLLRAAGFLL 297
            +YTAS+ SGAAFCRSAALIL+ALLLLRHALTIT D DG +DD S+  S+ LLRAAGFLL
Sbjct: 207 VEYTASSASGAAFCRSAALILMALLLLRHALTITDDTDGEEDDPSSILSLVLLRAAGFLL 248

Query: 298 PCYIMAWAISILQRRRQRQEAAALAATQVAFVLQHGQHRGLQFAIA 339
           PCYIMAWAISILQRRRQRQEAAAL ATQ A VLQ GQ R + F ++
Sbjct: 267 PCYIMAWAISILQRRRQRQEAAAL-ATQFALVLQSGQPRTVHFTVS 248

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029952.19.5e-211100.00E3 ubiquitin-protein ligase MARCH3 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946694.11.1e-16382.80uncharacterized protein LOC111450687 [Cucurbita moschata][more]
XP_023547303.18.2e-16282.01uncharacterized protein LOC111806165 [Cucurbita pepo subsp. pepo][more]
KAG6598983.16.9e-16182.43putative apyrase 6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022972852.13.1e-15380.82uncharacterized protein LOC111471364 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1G4K75.5e-16482.80uncharacterized protein LOC111450687 OS=Cucurbita moschata OX=3662 GN=LOC1114506... [more]
A0A6J1I5Y11.5e-15380.82uncharacterized protein LOC111471364 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1IBB61.3e-15280.72uncharacterized protein LOC111471364 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1CTP21.2e-14276.40uncharacterized protein LOC111014120 OS=Momordica charantia OX=3673 GN=LOC111014... [more]
A0A6J1G9V29.5e-14075.28uncharacterized protein LOC111452273 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT3G47550.37.3e-7648.04RING/FYVE/PHD zinc finger superfamily protein [more]
AT3G47550.67.3e-7648.04RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.24.0e-7455.24RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.44.0e-7455.24RING/FYVE/PHD zinc finger superfamily protein [more]
AT2G02960.14.0e-7455.24RING/FYVE/PHD zinc finger superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 220..240
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 350..372
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 339..380
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 17..43
NoneNo IPR availablePANTHERPTHR23012:SF181E3 UBIQUITIN-PROTEIN LIGASE MARCH-RELATEDcoord: 1..125
coord: 182..336
NoneNo IPR availableCDDcd16495RING_CH-C4HC3_MARCHcoord: 74..120
e-value: 4.5354E-19
score: 78.138
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 72..123
IPR011016Zinc finger, RING-CH-typeSMARTSM00744ringv_2coord: 73..120
e-value: 3.7E-20
score: 83.0
IPR011016Zinc finger, RING-CH-typePFAMPF12906RINGvcoord: 74..119
e-value: 7.7E-13
score: 48.4
IPR011016Zinc finger, RING-CH-typePROSITEPS51292ZF_RING_CHcoord: 66..126
score: 21.187059
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 60..127
e-value: 1.8E-18
score: 67.9
IPR022143Protein of unknown function DUF3675PFAMPF12428DUF3675coord: 186..301
e-value: 9.4E-42
score: 142.3
IPR033275E3 ubiquitin-protein ligase MARCH-likePANTHERPTHR23012RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAININGcoord: 1..125
IPR033275E3 ubiquitin-protein ligase MARCH-likePANTHERPTHR23012RING/FYVE/PHD ZINC FINGER DOMAIN-CONTAININGcoord: 182..336

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25814-RACarg25814-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004842 ubiquitin-protein transferase activity
molecular_function GO:0008270 zinc ion binding