Carg25813 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25813
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	E3 ubiquitin-protein ligase RING1-like
Location	Carg_Chr05: 6032145 .. 6032579 (+)
RNA-Seq Expression	Carg25813
Synteny	Carg25813

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAACGAAAATGGAAGCGAGAGGATATTGGTGGTGAACCTTGTTACACAGAGTATGATTGTGATCGAGGGACGTTCGAGGTTGGAAGCGCTATTGCGCGATTTGGGTGGGAAGGAGGGCTACCCATCGGCCTCGAAGGCGTCCATACAAGCGCTTCCCACCGTTCAGCTCAAGGCAGAGGAGGTGCCTTGCGAGTGCCCCATCTGTTTGAACGAGTGGGAGATGGGTTGTTTAGCCAAGGAGCTGCCTTGCACACACAAATTCCATCGCGATTGTATACAGAAATGGCTTGGCATCCATGGGACATGTCCGGTTTGTCGGTACAAGATGCCTGTTGATGAGAAGGATAACGACGGCGGAGTGGAGGCAAGGAGGGCGAGGGAGATTTGGGTTGCCTTTTCTTTCAATAACGATAGGAGAGCTGAGGGTTAG

mRNA sequence

ATGGAAAACGAAAATGGAAGCGAGAGGATATTGGTGGTGAACCTTGTTACACAGAGTATGATTGTGATCGAGGGACGTTCGAGGTTGGAAGCGCTATTGCGCGATTTGGGTGGGAAGGAGGGCTACCCATCGGCCTCGAAGGCGTCCATACAAGCGCTTCCCACCGTTCAGCTCAAGGCAGAGGAGGTGCCTTGCGAGTGCCCCATCTGTTTGAACGAGTGGGAGATGGGTTGTTTAGCCAAGGAGCTGCCTTGCACACACAAATTCCATCGCGATTGTATACAGAAATGGCTTGGCATCCATGGGACATGTCCGGTTTGTCGGTACAAGATGCCTGTTGATGAGAAGGATAACGACGGCGGAGTGGAGGCAAGGAGGGCGAGGGAGATTTGGGTTGCCTTTTCTTTCAATAACGATAGGAGAGCTGAGGGTTAG

Coding sequence (CDS)

ATGGAAAACGAAAATGGAAGCGAGAGGATATTGGTGGTGAACCTTGTTACACAGAGTATGATTGTGATCGAGGGACGTTCGAGGTTGGAAGCGCTATTGCGCGATTTGGGTGGGAAGGAGGGCTACCCATCGGCCTCGAAGGCGTCCATACAAGCGCTTCCCACCGTTCAGCTCAAGGCAGAGGAGGTGCCTTGCGAGTGCCCCATCTGTTTGAACGAGTGGGAGATGGGTTGTTTAGCCAAGGAGCTGCCTTGCACACACAAATTCCATCGCGATTGTATACAGAAATGGCTTGGCATCCATGGGACATGTCCGGTTTGTCGGTACAAGATGCCTGTTGATGAGAAGGATAACGACGGCGGAGTGGAGGCAAGGAGGGCGAGGGAGATTTGGGTTGCCTTTTCTTTCAATAACGATAGGAGAGCTGAGGGTTAG

Protein sequence

MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAEEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDGGVEARRAREIWVAFSFNNDRRAEG

Homology

[Close]

BLAST of Carg25813 vs. NCBI nr
Match: KAG6598984.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029953.1 E3 ubiquitin-protein ligase RING1-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305.4 bits (781), Expect = 2.5e-79
Identity = 144/144 (100.00%), Postives = 144/144 (100.00%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG
Sbjct: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNNDRRAEG
Sbjct: 121 GVEARRAREIWVAFSFNNDRRAEG 144

[Close]

BLAST of Carg25813 vs. NCBI nr
Match: XP_022946696.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata])

HSP 1 Score: 293.9 bits (751), Expect = 7.6e-76
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEG+PSASKASIQALPTVQLKA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGHPSASKASIQALPTVQLKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EE+PCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEK+N G
Sbjct: 61  EELPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKENAG 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNND R EG
Sbjct: 121 GVEARRAREIWVAFSFNNDARVEG 144

[Close]

BLAST of Carg25813 vs. NCBI nr
Match: XP_022972862.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima])

HSP 1 Score: 290.8 bits (743), Expect = 6.4e-75
Identity = 134/144 (93.06%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEG+PSASKASIQALPTVQ+KA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGHPSASKASIQALPTVQVKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EE+PCECP+CLNEWE+GCLAKELPCTHKFHR+CIQKWLGIHGTCPVCRYKMPVDE++ND 
Sbjct: 61  EELPCECPVCLNEWEIGCLAKELPCTHKFHRECIQKWLGIHGTCPVCRYKMPVDEEENDA 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNNDRR EG
Sbjct: 121 GVEARRAREIWVAFSFNNDRRVEG 144

[Close]

BLAST of Carg25813 vs. NCBI nr
Match: XP_023546717.1 (E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.8 bits (730), Expect = 2.1e-73
Identity = 134/144 (93.06%), Postives = 139/144 (96.53%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEG+PSASKASIQALPTVQ+KA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGHPSASKASIQALPTVQVKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EE+PCECPICLNEWEMGCLA +LPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEK+N  
Sbjct: 61  EELPCECPICLNEWEMGCLANQLPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKENGV 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNND R EG
Sbjct: 121 GVEARRAREIWVAFSFNNDTRFEG 144

[Close]

BLAST of Carg25813 vs. NCBI nr
Match: XP_023523370.1 (E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 216.5 bits (550), Expect = 1.5e-52
Identity = 101/145 (69.66%), Postives = 121/145 (83.45%), Query Frame = 0


Query: 2   ENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAE 61
           +N N S+RI +VN  TQSM+VIEGRS LEAL+RDLGGK+G P AS+ASI+ALPTVQ+ AE
Sbjct: 88  QNANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAE 147

Query: 62  EVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMP--VDEKDND 121
           E+ CEC ICL+EWE+G +AKELPC HKFH DCI+KWLG+HG CP+CR K+P  VD K+N+
Sbjct: 148 ELGCECVICLDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNE 207

Query: 122 GGVEARRAREIWVAFSFNNDRRAEG 145
           G    RRAREIWV FSFNNDRR+EG
Sbjct: 208 G----RRAREIWVGFSFNNDRRSEG 228

[Close]

BLAST of Carg25813 vs. ExPASy Swiss-Prot
Match: Q9LQX2 (E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 1.3e-33
Identity = 76/141 (53.90%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 8   ERILVVNLVTQSMIVIEGRSRLEALLRDL--GGKEGYPSASKASIQALPTVQLKAEEVPC 67
           ERI++VN  TQ MIV+EG S +  LLR L    +EG P ASKASI A+P V++   E   
Sbjct: 54  ERIILVNPFTQGMIVLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEIDGCE--G 113

Query: 68  ECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVD-----EKDNDG 127
           EC ICL EW+     KE+PC H+FH  CI+KWLG HG+CPVCRY+MPVD     +K NDG
Sbjct: 114 ECVICLEEWKSEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRNDG 173

Query: 128 GVEARRAREIWVAFSFNNDRR 142
                   EIWV FSFN+ RR
Sbjct: 174 -------NEIWVRFSFNDGRR 185

[Close]

BLAST of Carg25813 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 4.4e-18
Identity = 40/96 (41.67%), Postives = 63/96 (65.62%), Query Frame = 0


Query: 29  LEALLRDLG----GKEGYPSASKASIQALPTVQLKAEEVPCE---CPICLNEWEMGCLAK 88
           LE L++ L      + G P ASK++I ALPTV++  + +  E   C +C++E+E G   K
Sbjct: 171 LEQLIQQLAENDPNRYGTPPASKSAIDALPTVKVTKDMLKSEMNQCAVCMDEFEDGSDVK 230

Query: 89  ELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKD 118
           ++PC H FH+DC+  WL +H +CPVCR+++P D+ D
Sbjct: 231 QMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPD 266

[Close]

BLAST of Carg25813 vs. ExPASy Swiss-Prot
Match: P0CH30 (E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-17
Identity = 38/96 (39.58%), Postives = 62/96 (64.58%), Query Frame = 0


Query: 29  LEALLRDLG----GKEGYPSASKASIQALPTVQLKAEEVPCE---CPICLNEWEMGCLAK 88
           LE L++ L      + G P ASK++I+ALP V +    +  E   C +C++++E G  AK
Sbjct: 179 LEQLIQQLAENDPNRYGTPPASKSAIEALPLVNITKSNLNSEFNQCAVCMDDFEEGTEAK 238

Query: 89  ELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKD 118
           ++PC H +H+DC+  WL +H +CPVCR+++P D+ D
Sbjct: 239 QMPCKHLYHKDCLLPWLELHNSCPVCRHELPTDDPD 274

[Close]

BLAST of Carg25813 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-17
Identity = 40/87 (45.98%), Postives = 57/87 (65.52%), Query Frame = 0


Query: 29  LEALLRDLGGKEGYPSASKASIQALPTVQLKAEEVPCECPICLNEWEMGCLAKELPCTHK 88
           L+ L      + G P A K +++ALPTV  K EEV   C +CL++ E+G  AK++PC HK
Sbjct: 164 LQHLAESDPSRNGTPPAKKEAVEALPTV--KIEEV-VSCSVCLDDLEVGSQAKQMPCEHK 223

Query: 89  FHRDCIQKWLGIHGTCPVCRYKMPVDE 116
           FH  CI  WL +H +CPVCR+++P +E
Sbjct: 224 FHSSCILPWLELHSSCPVCRFELPSEE 247

[Close]

BLAST of Carg25813 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 9.2e-16
Identity = 41/112 (36.61%), Postives = 61/112 (54.46%), Query Frame = 0


Query: 22  VIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAEEVPCE--CPICLNEWEMGCL 81
           ++E  S++EA    + G+ G P ASK++I++LP V++       E  C +C   +E G  
Sbjct: 169 LLEQLSQIEASGNGI-GRSGNPPASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAGIE 228

Query: 82  AKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDGGVEARRAREIW 132
            +E+PC H FH DCI  WL I  +CPVCR+++P D        E      IW
Sbjct: 229 GREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRSNEEEHAVGMTIW 279

[Close]

BLAST of Carg25813 vs. ExPASy TrEMBL
Match: A0A6J1G4D3 (E3 ubiquitin-protein ligase RING1-like OS=Cucurbita moschata OX=3662 GN=LOC111450688 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.7e-76
Identity = 138/144 (95.83%), Postives = 141/144 (97.92%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEG+PSASKASIQALPTVQLKA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGHPSASKASIQALPTVQLKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EE+PCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEK+N G
Sbjct: 61  EELPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKENAG 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNND R EG
Sbjct: 121 GVEARRAREIWVAFSFNNDARVEG 144

[Close]

BLAST of Carg25813 vs. ExPASy TrEMBL
Match: A0A6J1I5Z1 (E3 ubiquitin-protein ligase RING1-like OS=Cucurbita maxima OX=3661 GN=LOC111471372 PE=4 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 3.1e-75
Identity = 134/144 (93.06%), Postives = 142/144 (98.61%), Query Frame = 0


Query: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKA 60
           MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEG+PSASKASIQALPTVQ+KA
Sbjct: 1   MENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGHPSASKASIQALPTVQVKA 60

Query: 61  EEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDG 120
           EE+PCECP+CLNEWE+GCLAKELPCTHKFHR+CIQKWLGIHGTCPVCRYKMPVDE++ND 
Sbjct: 61  EELPCECPVCLNEWEIGCLAKELPCTHKFHRECIQKWLGIHGTCPVCRYKMPVDEEENDA 120

Query: 121 GVEARRAREIWVAFSFNNDRRAEG 145
           GVEARRAREIWVAFSFNNDRR EG
Sbjct: 121 GVEARRAREIWVAFSFNNDRRVEG 144

[Close]

BLAST of Carg25813 vs. ExPASy TrEMBL
Match: A0A6J1K8F1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492631 PE=4 SV=1)

HSP 1 Score: 216.5 bits (550), Expect = 7.4e-53
Identity = 101/145 (69.66%), Postives = 121/145 (83.45%), Query Frame = 0


Query: 2   ENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAE 61
           +N N S+RI +VN  TQSM+VIEGRS LEAL+RDLGGK+G P AS+ASI+ALPTVQ+ AE
Sbjct: 88  QNANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAE 147

Query: 62  EVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMP--VDEKDND 121
           E+ CEC ICL+EWE+G +AKELPC HKFH DCI+KWLG+HG CP+CR K+P  VD K+N+
Sbjct: 148 ELGCECVICLDEWEIGAVAKELPCGHKFHPDCIEKWLGVHGNCPLCRCKLPDDVDRKNNE 207

Query: 122 GGVEARRAREIWVAFSFNNDRRAEG 145
           G    RRAREIWV FSFNNDRR+EG
Sbjct: 208 G----RRAREIWVGFSFNNDRRSEG 228

[Close]

BLAST of Carg25813 vs. ExPASy TrEMBL
Match: A0A6J1GCB1 (E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC111452865 PE=4 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 3.7e-52
Identity = 101/145 (69.66%), Postives = 120/145 (82.76%), Query Frame = 0


Query: 2   ENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAE 61
           +N N S+RI +VN  TQSM+VIEGRS LEAL+RDLGGK+G P AS+ASI+ALPTVQ+ AE
Sbjct: 51  QNANRSDRIFLVNPFTQSMVVIEGRSSLEALMRDLGGKDGQPPASRASIEALPTVQIMAE 110

Query: 62  EVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMP--VDEKDND 121
           E  CEC ICL+EW++G +AKELPC HKFH DCI+KWLGIHG CP+CR K+P  VD K+N+
Sbjct: 111 ESGCECVICLDEWKIGAVAKELPCGHKFHPDCIEKWLGIHGNCPLCRCKLPDDVDRKNNE 170

Query: 122 GGVEARRAREIWVAFSFNNDRRAEG 145
           G    RRAREIWV FSFNNDRR+EG
Sbjct: 171 G----RRAREIWVGFSFNNDRRSEG 191

[Close]

BLAST of Carg25813 vs. ExPASy TrEMBL
Match: A0A6J1DR69 (E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC111023495 PE=4 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 2.8e-44
Identity = 92/140 (65.71%), Postives = 107/140 (76.43%), Query Frame = 0


Query: 7   SERILVVNLVTQSMIVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQLKAEEVPCE 66
           SER  +VN  TQSM+VIEG S LE+LLRDLG K+G P ASKASI+ALP VQL  E    E
Sbjct: 44  SERFFLVNPFTQSMVVIEGSSALESLLRDLGSKDGPPPASKASIEALPAVQLLEEG---E 103

Query: 67  CPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDE---KDNDGGVE 126
           C ICL+EWE+G  AKELPC HKFH +CI+KWLGIHG+CP+CR+KMPVD+   K ND    
Sbjct: 104 CVICLDEWEIGATAKELPCKHKFHPNCIEKWLGIHGSCPICRHKMPVDDGDGKKNDVVGR 163

Query: 127 ARRAREIWVAFSFNNDRRAE 144
            RR REIWV FSFNND R++
Sbjct: 164 GRRLREIWVGFSFNNDIRSD 180

[Close]

BLAST of Carg25813 vs. TAIR 10
Match: AT1G26800.1 (RING/U-box superfamily protein )

HSP 1 Score: 144.1 bits (362), Expect = 9.0e-35
Identity = 76/141 (53.90%), Postives = 92/141 (65.25%), Query Frame = 0


Query: 8   ERILVVNLVTQSMIVIEGRSRLEALLRDL--GGKEGYPSASKASIQALPTVQLKAEEVPC 67
           ERI++VN  TQ MIV+EG S +  LLR L    +EG P ASKASI A+P V++   E   
Sbjct: 54  ERIILVNPFTQGMIVLEGSSGMNPLLRSLLESREEGRPPASKASIDAMPIVEIDGCE--G 113

Query: 68  ECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVD-----EKDNDG 127
           EC ICL EW+     KE+PC H+FH  CI+KWLG HG+CPVCRY+MPVD     +K NDG
Sbjct: 114 ECVICLEEWKSEETVKEMPCKHRFHGGCIEKWLGFHGSCPVCRYEMPVDGDEIGKKRNDG 173

Query: 128 GVEARRAREIWVAFSFNNDRR 142
                   EIWV FSFN+ RR
Sbjct: 174 -------NEIWVRFSFNDGRR 185

[Close]

BLAST of Carg25813 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-24
Identity = 54/124 (43.55%), Postives = 75/124 (60.48%), Query Frame = 0


Query: 21  IVIEGRSRLEALLRDLGGKEGYPSASKASIQALPTVQL--KAEEVPCECPICLNEWEMGC 80
           ++I  RS L+    D GGK+G   A K+ ++ +P V +    E+    C ICL+EW  G 
Sbjct: 62  VMIGSRSGLDDFFSD-GGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGD 121

Query: 81  LAKELPCTHKFHRDCIQKWLGIHGTCPVCRYKMPVDEKDNDGGVEARRAREIWVAFSFN- 140
           +A E+PC HKFH  C+++WLG H TCP+CRY+MPV+E      VE  +   IW+ FS N 
Sbjct: 122 VAAEMPCKHKFHSKCVEEWLGRHATCPMCRYEMPVEE------VEEEKKIGIWIGFSINA 178

Query: 141 NDRR 142
            DRR
Sbjct: 182 GDRR 178

[Close]

BLAST of Carg25813 vs. TAIR 10
Match: AT5G56340.1 (RING/U-box superfamily protein )

HSP 1 Score: 98.2 bits (243), Expect = 5.7e-21
Identity = 46/138 (33.33%), Postives = 76/138 (55.07%), Query Frame = 0


Query: 2   ENENGSERILVVNLVTQSMIVIEGRSRLEALLRDLG--------------------GKEG 61
           +N    +R++++N   QS++V   +++    L  LG                     ++G
Sbjct: 177 DNSRERDRVIMINPYNQSLVVPSDQNQNHPSLTSLGDYFIGPGLDLLLQHLAENDPNRQG 236

Query: 62  YPSASKASIQALPTVQLKAEEVPCECPICLNEWEMGCLAKELPCTHKFHRDCIQKWLGIH 120
            P A K +++ALPTV++     P +C +CL+++E G  AKE+PC HKFH  CI  WL +H
Sbjct: 237 TPPARKEAVEALPTVKIME---PLQCSVCLDDFEKGTEAKEMPCKHKFHVRCIVPWLELH 296

[Close]

BLAST of Carg25813 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 95.5 bits (236), Expect = 3.7e-20
Identity = 40/91 (43.96%), Postives = 60/91 (65.93%), Query Frame = 0


Query: 29  LEALLRDLGGKEGYPSASKASIQALPTVQLKAEEVPCECPICLNEWEMGCLAKELPCTHK 88
           L+ L  +   + G P A K +++AL TV++   E   +C +CL+++E+G  AK +PCTHK
Sbjct: 188 LQRLAENDPNRYGTPPAKKEAVEALATVKI---EETLQCSVCLDDFEIGTEAKLMPCTHK 247

Query: 89  FHRDCIQKWLGIHGTCPVCRYKMPVDEKDND 120
           FH DC+  WL +H +CPVCRY++P DE   D
Sbjct: 248 FHSDCLLPWLELHSSCPVCRYQLPADEAKTD 275

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BLAST of Carg25813 vs. TAIR 10
Match: AT3G13430.1 (RING/U-box superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-19
Identity = 38/83 (45.78%), Postives = 56/83 (67.47%), Query Frame = 0


Query: 35  DLGGKEGYPSASKASIQALPTVQLKAEEVPCECPICLNEWEMGCLAKELPCTHKFHRDCI 94
           DL  + G P A+K +++AL  V  K E+   +C +CL+++E+G  AKE+PC HKFH DC+
Sbjct: 195 DLNNRYGTPPATKEAVEALAMV--KIEDSLLQCSVCLDDFEIGMEAKEMPCKHKFHSDCL 254

Query: 95  QKWLGIHGTCPVCRYKMPVDEKD 118
             WL +H +CPVCRY +P  + D
Sbjct: 255 LPWLELHSSCPVCRYLLPTGDDD 275

The following BLAST results are available for this feature:

BLAST of Carg25813 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6598984.1	2.5e-79	100.00	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022946696.1	7.6e-76	95.83	E3 ubiquitin-protein ligase RING1-like [Cucurbita moschata]	[more]
XP_022972862.1	6.4e-75	93.06	E3 ubiquitin-protein ligase RING1-like [Cucurbita maxima]	[more]
XP_023546717.1	2.1e-73	93.06	E3 ubiquitin-protein ligase RING1-like [Cucurbita pepo subsp. pepo]	[more]
XP_023523370.1	1.5e-52	69.66	E3 ubiquitin-protein ligase RNF181-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg25813 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQX2	1.3e-33	53.90	E3 ubiquitin-protein ligase MPSR1 OS=Arabidopsis thaliana OX=3702 GN=MPSR1 PE=1 ...	[more]
Q8LPN7	4.4e-18	41.67	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]
P0CH30	1.7e-17	39.58	E3 ubiquitin-protein ligase RING1 OS=Gossypium hirsutum OX=3635 GN=RING1 PE=1 SV...	[more]
Q6AVN2	1.7e-17	45.98	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q9SNB6	9.2e-16	36.61	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]

BLAST of Carg25813 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4D3	3.7e-76	95.83	E3 ubiquitin-protein ligase RING1-like OS=Cucurbita moschata OX=3662 GN=LOC11145...	[more]
A0A6J1I5Z1	3.1e-75	93.06	E3 ubiquitin-protein ligase RING1-like OS=Cucurbita maxima OX=3661 GN=LOC1114713...	[more]
A0A6J1K8F1	7.4e-53	69.66	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita maxima OX=3661 GN=LOC111492...	[more]
A0A6J1GCB1	3.7e-52	69.66	E3 ubiquitin-protein ligase RNF181-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DR69	2.8e-44	65.71	E3 ubiquitin-protein ligase RNF181 OS=Momordica charantia OX=3673 GN=LOC11102349...	[more]

BLAST of Carg25813 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G26800.1	9.0e-35	53.90	RING/U-box superfamily protein	[more]
AT1G14200.1	1.4e-24	43.55	RING/U-box superfamily protein	[more]
AT5G56340.1	5.7e-21	33.33	RING/U-box superfamily protein	[more]
AT1G55530.1	3.7e-20	43.96	RING/U-box superfamily protein	[more]
AT3G13430.1	1.8e-19	45.78	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 67..107 e-value: 6.8E-7 score: 38.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 66..108 e-value: 1.1E-11 score: 44.9
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 67..108 score: 12.223724
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 36..114 e-value: 7.3E-21 score: 75.6
None	No IPR available	PANTHER	PTHR15710:SF100	RING/U-BOX SUPERFAMILY PROTEIN-RELATED	coord: 6..141
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 6..141
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 56..114

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25813-RA	Carg25813-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity