Carg25767 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25767
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	monooxygenase 2-like
Location	Carg_Chr19: 7235101 .. 7235929 (-)
RNA-Seq Expression	Carg25767
Synteny	Carg25767

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGAAAAAAGGTGTTGATTGGGTGTGATGGAGTGAAGTCTGTGGTTGCTAAGTGGCTGGGCTTCAAGAAGCCTTCTTTGTCAGGCAGAAACGCTACTAGAGGGATTACAACATACGAGAATGGCCATGGATTTGAGAATAATTTCATGTGGTTTTTCAGCAAAGGCCTTCGCTTTGGTGTTATGCCATGTAATGCCAACACTGTCTATTGGTTTAGCACTTGGCATTCTTCCAAACAAGGTTAGCCCCTTGGAACACGAGAAATCGTTAGACACACCTTTCTTAACGTCGTAATAATACTCGTGATATCTTCTTGTATTGTATTCCTAACACATTGTGAAACTCGAAGGTAAGGAGATTGAAGAGAACTCAGTTAGACTAAAGCAACATATTTTGAGCAAGCTCGAAAAGGTACCTGATCGAGTAAGGGCCGTTGTTGAAGACACTAAAGTTGATTCTTTTGTGTCGTTGCCATTGAGATGTAGACATCCTTGGGAGCTGATTTGGAATGACTTTTCTCGAGGTAACGTTTGTATTGCAGGCGACGCGCTTCACTCGATGACCCCTGATCTCGGACAAGGAGGTTGTTCTGCATTGGAAGATGGCGTCGTTCTTGCTCGATGTTTAGCCGAAGCGATGTTGAGGAATCCAATGGGAGGAGGAGAAGAGGAATATAAGAGAATTGAGAAGGGGTTAGAGAAATATGCAAAAGAAAGGAGATGGAGATGCATTAAGCTCATTGCTGCATCTTATGTGGTTGGTTCAATTCAAGAGAGTGAAGGGAAATTAATGAATTACTTGAGGGATAACATCTTGGCTGATTTTCTTGTT

mRNA sequence

TGAAAAAAGGTGTTGATTGGGTGTGATGGAGTGAAGTCTGTGGTTGCTAAGTGGCTGGGCTTCAAGAAGCCTTCTTTGTCAGGCAGAAACGCTACTAGAGGGATTACAACATACGAGAATGGCCATGGATTTGAGAATAATTTCATGTGGTTTTTCAGCAAAGGCCTTCGCTTTGGTGTTATGCCATGTAATGCCAACACTGTCTATTGGTTTAGCACTTGGCATTCTTCCAAACAAGGTAAGGAGATTGAAGAGAACTCAGTTAGACTAAAGCAACATATTTTGAGCAAGCTCGAAAAGGTACCTGATCGAGTAAGGGCCGTTGTTGAAGACACTAAAGTTGATTCTTTTGTGTCGTTGCCATTGAGATGTAGACATCCTTGGGAGCTGATTTGGAATGACTTTTCTCGAGGTAACGTTTGTATTGCAGGCGACGCGCTTCACTCGATGACCCCTGATCTCGGACAAGGAGGTTGTTCTGCATTGGAAGATGGCGTCGTTCTTGCTCGATGTTTAGCCGAAGCGATGTTGAGGAATCCAATGGGAGGAGGAGAAGAGGAATATAAGAGAATTGAGAAGGGGTTAGAGAAATATGCAAAAGAAAGGAGATGGAGATGCATTAAGCTCATTGCTGCATCTTATGTGGTTGGTTCAATTCAAGAGAGTGAAGGGAAATTAATGAATTACTTGAGGGATAACATCTTGGCTGATTTTCTTGTT

Coding sequence (CDS)

ATGTGGTTTTTCAGCAAAGGCCTTCGCTTTGGTGTTATGCCATGTAATGCCAACACTGTCTATTGGTTTAGCACTTGGCATTCTTCCAAACAAGGTAAGGAGATTGAAGAGAACTCAGTTAGACTAAAGCAACATATTTTGAGCAAGCTCGAAAAGGTACCTGATCGAGTAAGGGCCGTTGTTGAAGACACTAAAGTTGATTCTTTTGTGTCGTTGCCATTGAGATGTAGACATCCTTGGGAGCTGATTTGGAATGACTTTTCTCGAGGTAACGTTTGTATTGCAGGCGACGCGCTTCACTCGATGACCCCTGATCTCGGACAAGGAGGTTGTTCTGCATTGGAAGATGGCGTCGTTCTTGCTCGATGTTTAGCCGAAGCGATGTTGAGGAATCCAATGGGAGGAGGAGAAGAGGAATATAAGAGAATTGAGAAGGGGTTAGAGAAATATGCAAAAGAAAGGAGATGGAGATGCATTAAGCTCATTGCTGCATCTTATGTGGTTGGTTCAATTCAAGAGAGTGAAGGGAAATTAATGAATTACTTGAGGGATAACATCTTGGCTGATTTTCTTGTT

Protein sequence

MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNYLRDNILADFLV

Homology

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BLAST of Carg25767 vs. NCBI nr
Match: KAG7011775.1 (hpxO, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 401.0 bits (1029), Expect = 5.9e-108
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV
Sbjct: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN
Sbjct: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 181 YLRDNILADFLV 192

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BLAST of Carg25767 vs. NCBI nr
Match: XP_022968891.1 (monooxygenase 2-like, partial [Cucurbita maxima])

HSP 1 Score: 389.8 bits (1000), Expect = 1.4e-104
Identity = 185/192 (96.35%), Postives = 188/192 (97.92%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 221 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 280

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VED+KVDSFVSLPLRCRHPWEL+WNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 281 VEDSKVDSFVSLPLRCRHPWELVWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 340

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLA+AMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLI  SYVVGSIQESEGKLMN
Sbjct: 341 ARCLAKAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLITTSYVVGSIQESEGKLMN 400

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 401 YLRDNILADFLV 412

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BLAST of Carg25767 vs. NCBI nr
Match: XP_023554695.1 (monooxygenase 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 389.0 bits (998), Expect = 2.3e-104
Identity = 186/192 (96.88%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 198 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 257

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDTKVDSFVSLPLRCR PWEL+WNDF RGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 258 VEDTKVDSFVSLPLRCRQPWELVWNDFFRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 317

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIA SYVVGSIQESEGKLMN
Sbjct: 318 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIATSYVVGSIQESEGKLMN 377

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 378 YLRDNILADFLV 389

[Close]

BLAST of Carg25767 vs. NCBI nr
Match: KAG6572121.1 (Monooxygenase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 389.0 bits (998), Expect = 2.3e-104
Identity = 186/192 (96.88%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 198 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 257

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDTKVDSFVSLPLRCR PWEL+WNDF RGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 258 VEDTKVDSFVSLPLRCRQPWELVWNDFFRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 317

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLI ASYVVGSIQESEGKLMN
Sbjct: 318 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLITASYVVGSIQESEGKLMN 377

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 378 YLRDNILADFLV 389

[Close]

BLAST of Carg25767 vs. NCBI nr
Match: XP_022953131.1 (monooxygenase 2-like isoform X1 [Cucurbita moschata] >XP_022953132.1 monooxygenase 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 385.6 bits (989), Expect = 2.6e-103
Identity = 184/192 (95.83%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 198 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 257

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDTKVDSFVSLPLRCR PWEL+WNDF RGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 258 VEDTKVDSFVSLPLRCRQPWELVWNDFFRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 317

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLAEAMLR+P+GGGEEEYKRIEKGLEKYAKERRWRCIKLIA SYVVGSIQESEGKLMN
Sbjct: 318 ARCLAEAMLRSPIGGGEEEYKRIEKGLEKYAKERRWRCIKLIATSYVVGSIQESEGKLMN 377

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 378 YLRDNILADFLV 389

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BLAST of Carg25767 vs. ExPASy Swiss-Prot
Match: O81816 (Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 1.1e-53
Identity = 103/194 (53.09%), Postives = 137/194 (70.62%), Query Frame = 0


Query: 3   FFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVE 62
           F+  G+R G +PC+ NTVYWF T H+S    E E NS  LK+ +L+K++ +P+ ++ VVE
Sbjct: 199 FYGDGVRSGFIPCDHNTVYWFLT-HTSTDIDE-ETNSEILKEFVLNKIKDLPENIKNVVE 258

Query: 63  DTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLAR 122
            T +DS V   L+ R PWEL+W++ ++ NVC+AGDALH MTPD+GQGGCSA+EDGV+LAR
Sbjct: 259 TTDLDSMVMSQLKYRPPWELLWSNITKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILAR 318

Query: 123 CLAEAMLRNPMGGGEEE-----YKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGK 182
           CL EA+    + G  EE     YKRIE+GL+KYA ER+WR I LI  +Y VG IQ+S GK
Sbjct: 319 CLGEAIKAKSLKGETEENEEEGYKRIEEGLKKYAGERKWRSIDLITTAYTVGFIQQSRGK 378

Query: 183 LMNYLRDNILADFL 192
            MN  RD  L+ +L
Sbjct: 379 WMNMFRDRFLSSYL 390

[Close]

BLAST of Carg25767 vs. ExPASy Swiss-Prot
Match: Q9FLC2 (Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 2.8e-52
Identity = 98/193 (50.78%), Postives = 132/193 (68.39%), Query Frame = 0


Query: 3   FFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVE 62
           F+  G+R G + C+ NTVYWF T  S+   K   +N  ++KQ +L+K++ +PD +++++E
Sbjct: 199 FYGNGVRSGFISCDQNTVYWFLTHTSTDLDK---KNHQKIKQFVLTKIKDLPDNIKSILE 258

Query: 63  DTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLAR 122
            T +DS V  PL  R PWEL+W + ++ NVC+AGDALH MTPD+GQGGCSA+EDGV+LAR
Sbjct: 259 TTDLDSLVMNPLMYRPPWELLWANIAKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILAR 318

Query: 123 CLAEAMLRNPMGGGEEE----YKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKL 182
           CL EAM    M G  E+    Y+RIE GL+KYA  R+WR I LI  SY VG IQ+S GK 
Sbjct: 319 CLGEAMKAKNMKGETEDENESYRRIEDGLKKYAGSRKWRSIDLITTSYTVGFIQQSRGKW 378

Query: 183 MNYLRDNILADFL 192
           M   RD  ++ FL
Sbjct: 379 MTLFRDKFMSSFL 388

[Close]

BLAST of Carg25767 vs. ExPASy Swiss-Prot
Match: O81815 (Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 1.3e-17
Identity = 57/176 (32.39%), Postives = 90/176 (51.14%), Query Frame = 0


Query: 11  GVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSK-LEKVPDRVRAVVEDTKVDSF 70
           G +P   N V+WF            ++ S+    ++  K  + + +  + +V+   V+S 
Sbjct: 203 GRLPLTDNQVFWFLVHMQDNNHNGKDQESI---ANLCRKWADDLSEDWKEMVKICNVESL 262

Query: 71  VSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLARCLAEAML 130
               LR R P E++   F RG V +AGDA+H M P L QGG +ALED VVLARCLA  + 
Sbjct: 263 TLTHLRYRAPSEIMLGKFRRGTVTVAGDAMHVMGPFLAQGGSAALEDAVVLARCLARKV- 322

Query: 131 RNPMGGG---EEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNYL 183
             P  G    +   K IE+ +++Y  ERR R + L   +Y+ G   ++  K++  +
Sbjct: 323 -GPDHGDLLKDCSMKNIEEAIDEYVDERRMRLLGLSVQTYLTGRSLQTSSKVLRLM 373

[Close]

BLAST of Carg25767 vs. ExPASy Swiss-Prot
Match: H1ZZA4 (Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.2e-15
Identity = 54/182 (29.67%), Postives = 85/182 (46.70%), Query Frame = 0


Query: 7   GLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVEDTKV 66
           G+RFGV+P      YWF+T  + + G +  +    L Q        +P     ++E+T  
Sbjct: 203 GMRFGVVPIGEGQTYWFATATAPEGGVDHPDARTELLQRFSGWHAPIPQ----LIENTPS 262

Query: 67  DSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLARCLA- 126
            + +   +  R P       + +G   + GDA H MTP++GQGGC A+ED VVLARCL+ 
Sbjct: 263 SAIMRTDIHDRVP----IRQWVQGRAVLLGDAAHPMTPNMGQGGCQAVEDAVVLARCLSL 322

Query: 127 EAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNYLRDN 186
           EA               +   L +Y   R  R    +A SY +G I + E     ++R+ 
Sbjct: 323 EA--------------ELPAALARYQAVRVERANDFVAGSYRIGQIGQWENAFACWVREK 362

Query: 187 IL 188
           ++
Sbjct: 383 LM 362

[Close]

BLAST of Carg25767 vs. ExPASy Swiss-Prot
Match: Q6F6Y2 (FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=hpxO PE=1 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 2.5e-08
Identity = 43/160 (26.88%), Postives = 71/160 (44.38%), Query Frame = 0


Query: 3   FFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVE 62
           +  +G R  +MP   N  Y+F           +E +  + K+ + S  +    +V+ ++E
Sbjct: 198 YVGEGKRASLMPVADNRFYFFL---DVPLEAGLENDKCKYKETLQSYFKGWCPQVQTLIE 257

Query: 63  DTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLAR 122
                    + +    P+      F +G V + GDA HS TPD+GQGGC A+ED + LAR
Sbjct: 258 RLDPQKTNRVEICDIEPFA----QFYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLAR 317

Query: 123 CLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLI 163
            L    L             +E  L +Y ++R  R  +L+
Sbjct: 318 SLQINTL------------SVEDALRRYQEKRNQRANELV 338

[Close]

BLAST of Carg25767 vs. ExPASy TrEMBL
Match: A0A6J1HW45 (monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111468075 PE=3 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 6.5e-105
Identity = 185/192 (96.35%), Postives = 188/192 (97.92%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 221 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 280

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VED+KVDSFVSLPLRCRHPWEL+WNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 281 VEDSKVDSFVSLPLRCRHPWELVWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 340

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLA+AMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLI  SYVVGSIQESEGKLMN
Sbjct: 341 ARCLAKAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLITTSYVVGSIQESEGKLMN 400

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 401 YLRDNILADFLV 412

[Close]

BLAST of Carg25767 vs. ExPASy TrEMBL
Match: A0A6J1GMD2 (monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455625 PE=3 SV=1)

HSP 1 Score: 385.6 bits (989), Expect = 1.2e-103
Identity = 184/192 (95.83%), Postives = 187/192 (97.40%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEEN VRLKQHILSKL KVPDRVRAV
Sbjct: 198 MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAV 257

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDTKVDSFVSLPLRCR PWEL+WNDF RGNVCIAGDALHSMTPDLGQGGCSALEDGVVL
Sbjct: 258 VEDTKVDSFVSLPLRCRQPWELVWNDFFRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 317

Query: 121 ARCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMN 180
           ARCLAEAMLR+P+GGGEEEYKRIEKGLEKYAKERRWRCIKLIA SYVVGSIQESEGKLMN
Sbjct: 318 ARCLAEAMLRSPIGGGEEEYKRIEKGLEKYAKERRWRCIKLIATSYVVGSIQESEGKLMN 377

Query: 181 YLRDNILADFLV 193
           YLRDNILADFLV
Sbjct: 378 YLRDNILADFLV 389

[Close]

BLAST of Carg25767 vs. ExPASy TrEMBL
Match: A0A5A7U5X4 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G001200 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.8e-86
Identity = 161/194 (82.99%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCN+NTVYWF+TW  SKQ +EIEEN  +LK+HILSKL KVPD+ RAV
Sbjct: 200 MWFFSKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAV 259

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDT+VD+FVSLPLR R PWEL+ N+FSRGNV IAGDALHSMTPDLGQGGCSALEDGV+L
Sbjct: 260 VEDTQVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVIL 319

Query: 121 ARCLAEAMLRNPMGGGE--EEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKL 180
           ARCLAEAM RNP G  E  EEYKRIEKGLEKYAKERRWR IKLI ASYVVGSIQES+GK+
Sbjct: 320 ARCLAEAMSRNPNGEVEDKEEYKRIEKGLEKYAKERRWRSIKLIIASYVVGSIQESKGKV 379

Query: 181 MNYLRDNILADFLV 193
           MNYLRDNILAD LV
Sbjct: 380 MNYLRDNILADSLV 393

[Close]

BLAST of Carg25767 vs. ExPASy TrEMBL
Match: A0A5D3CL17 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00440 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.8e-86
Identity = 161/194 (82.99%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCN+NTVYWF+TW  SKQ +EIEEN  +LK+HILSKL KVPD+ RAV
Sbjct: 180 MWFFSKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAV 239

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDT+VD+FVSLPLR R PWEL+ N+FSRGNV IAGDALHSMTPDLGQGGCSALEDGV+L
Sbjct: 240 VEDTQVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVIL 299

Query: 121 ARCLAEAMLRNPMGGGE--EEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKL 180
           ARCLAEAM RNP G  E  EEYKRIEKGLEKYAKERRWR IKLI ASYVVGSIQES+GK+
Sbjct: 300 ARCLAEAMSRNPNGEVEDKEEYKRIEKGLEKYAKERRWRSIKLIIASYVVGSIQESKGKV 359

Query: 181 MNYLRDNILADFLV 193
           MNYLRDNILAD LV
Sbjct: 360 MNYLRDNILADSLV 373

[Close]

BLAST of Carg25767 vs. ExPASy TrEMBL
Match: A0A1S3BIK9 (FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=3 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 6.8e-86
Identity = 161/194 (82.99%), Postives = 174/194 (89.69%), Query Frame = 0


Query: 1   MWFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAV 60
           MWFFSKGLRFGVMPCN+NTVYWF+TW  SKQ +EIEEN  +LK+HILSKL KVPD+ RAV
Sbjct: 200 MWFFSKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAV 259

Query: 61  VEDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVL 120
           VEDT+VD+FVSLPLR R PWEL+ N+FSRGNV IAGDALHSMTPDLGQGGCSALEDGV+L
Sbjct: 260 VEDTQVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVIL 319

Query: 121 ARCLAEAMLRNPMGGGE--EEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKL 180
           ARCLAEAM RNP G  E  EEYKRIEKGLEKYAKERRWR IKLI ASYVVGSIQES+GK+
Sbjct: 320 ARCLAEAMSRNPNGEVEDKEEYKRIEKGLEKYAKERRWRSIKLIIASYVVGSIQESKGKV 379

Query: 181 MNYLRDNILADFLV 193
           MNYLRDNILAD LV
Sbjct: 380 MNYLRDNILADSLV 393

[Close]

BLAST of Carg25767 vs. TAIR 10
Match: AT4G38540.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 211.1 bits (536), Expect = 8.0e-55
Identity = 103/194 (53.09%), Postives = 137/194 (70.62%), Query Frame = 0


Query: 3   FFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVE 62
           F+  G+R G +PC+ NTVYWF T H+S    E E NS  LK+ +L+K++ +P+ ++ VVE
Sbjct: 199 FYGDGVRSGFIPCDHNTVYWFLT-HTSTDIDE-ETNSEILKEFVLNKIKDLPENIKNVVE 258

Query: 63  DTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLAR 122
            T +DS V   L+ R PWEL+W++ ++ NVC+AGDALH MTPD+GQGGCSA+EDGV+LAR
Sbjct: 259 TTDLDSMVMSQLKYRPPWELLWSNITKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILAR 318

Query: 123 CLAEAMLRNPMGGGEEE-----YKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGK 182
           CL EA+    + G  EE     YKRIE+GL+KYA ER+WR I LI  +Y VG IQ+S GK
Sbjct: 319 CLGEAIKAKSLKGETEENEEEGYKRIEEGLKKYAGERKWRSIDLITTAYTVGFIQQSRGK 378

Query: 183 LMNYLRDNILADFL 192
            MN  RD  L+ +L
Sbjct: 379 WMNMFRDRFLSSYL 390

[Close]

BLAST of Carg25767 vs. TAIR 10
Match: AT5G05320.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 206.5 bits (524), Expect = 2.0e-53
Identity = 98/193 (50.78%), Postives = 132/193 (68.39%), Query Frame = 0


Query: 3   FFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVVE 62
           F+  G+R G + C+ NTVYWF T  S+   K   +N  ++KQ +L+K++ +PD +++++E
Sbjct: 199 FYGNGVRSGFISCDQNTVYWFLTHTSTDLDK---KNHQKIKQFVLTKIKDLPDNIKSILE 258

Query: 63  DTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLAR 122
            T +DS V  PL  R PWEL+W + ++ NVC+AGDALH MTPD+GQGGCSA+EDGV+LAR
Sbjct: 259 TTDLDSLVMNPLMYRPPWELLWANIAKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILAR 318

Query: 123 CLAEAMLRNPMGGGEEE----YKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKL 182
           CL EAM    M G  E+    Y+RIE GL+KYA  R+WR I LI  SY VG IQ+S GK 
Sbjct: 319 CLGEAMKAKNMKGETEDENESYRRIEDGLKKYAGSRKWRSIDLITTSYTVGFIQQSRGKW 378

Query: 183 MNYLRDNILADFL 192
           M   RD  ++ FL
Sbjct: 379 MTLFRDKFMSSFL 388

[Close]

BLAST of Carg25767 vs. TAIR 10
Match: AT2G29720.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 110.5 bits (275), Expect = 1.5e-24
Identity = 64/187 (34.22%), Postives = 104/187 (55.61%), Query Frame = 0


Query: 2   WFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVV 61
           + F +GLR G +P +A  VYWF T++S   G ++ + ++ L++     +   P+ ++ ++
Sbjct: 235 YIFGRGLRAGYVPVSATKVYWFITFNSPSLGPQMMDPAI-LRKEAKELVSTWPEDLQNLI 294

Query: 62  EDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLA 121
           + T  ++    PL  R  W  I    S+G V + GDA H MTP+LGQG C ALED V+LA
Sbjct: 295 DLTPDEAISRTPLADRWLWPGIAPSASKGRVVLVGDAWHPMTPNLGQGACCALEDSVLLA 354

Query: 122 RCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNY 181
             LA A     + GG E    +E  +E Y  ER  +  +L   + +VG + +S+  L+  
Sbjct: 355 NKLASA-----INGGTES---VEGAMESYRSERWSQVFRLTVLANLVGKLLQSDNPLVCS 412

Query: 182 LRDNILA 189
           +RDNI++
Sbjct: 415 VRDNIVS 412

[Close]

BLAST of Carg25767 vs. TAIR 10
Match: AT2G35660.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 65/186 (34.95%), Postives = 100/186 (53.76%), Query Frame = 0


Query: 2   WFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVV 61
           + + KG+R G +P +   VYWF  ++S   G +I + ++ LK+     +   P+ ++ ++
Sbjct: 237 YIYGKGIRAGYVPVSTTKVYWFICFNSPSLGPKITDPAI-LKKQAKELVSTWPEDLQNLI 296

Query: 62  EDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLA 121
           + T  ++    PL  R  W  I    S+G V + GDA H MTP+LGQG C ALED VVLA
Sbjct: 297 DLTPDETISRTPLVDRWLWPGIAPPASKGRVVLVGDAWHPMTPNLGQGACCALEDSVVLA 356

Query: 122 RCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNY 181
             LA     N + GG E    IE  +E Y  ER  R   L   + +VG++ + E  L+  
Sbjct: 357 NKLA-----NAINGGTES---IEVAMESYGSERWSRAFPLTVRANLVGALLQWENPLVCS 413

Query: 182 LRDNIL 188
           +R+NI+
Sbjct: 417 IRNNIV 413

[Close]

BLAST of Carg25767 vs. TAIR 10
Match: AT2G35660.2 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.2e-23
Identity = 65/186 (34.95%), Postives = 100/186 (53.76%), Query Frame = 0


Query: 2   WFFSKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENSVRLKQHILSKLEKVPDRVRAVV 61
           + + KG+R G +P +   VYWF  ++S   G +I + ++ LK+     +   P+ ++ ++
Sbjct: 123 YIYGKGIRAGYVPVSTTKVYWFICFNSPSLGPKITDPAI-LKKQAKELVSTWPEDLQNLI 182

Query: 62  EDTKVDSFVSLPLRCRHPWELIWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLA 121
           + T  ++    PL  R  W  I    S+G V + GDA H MTP+LGQG C ALED VVLA
Sbjct: 183 DLTPDETISRTPLVDRWLWPGIAPPASKGRVVLVGDAWHPMTPNLGQGACCALEDSVVLA 242

Query: 122 RCLAEAMLRNPMGGGEEEYKRIEKGLEKYAKERRWRCIKLIAASYVVGSIQESEGKLMNY 181
             LA     N + GG E    IE  +E Y  ER  R   L   + +VG++ + E  L+  
Sbjct: 243 NKLA-----NAINGGTES---IEVAMESYGSERWSRAFPLTVRANLVGALLQWENPLVCS 299

Query: 182 LRDNIL 188
           +R+NI+
Sbjct: 303 IRNNIV 299

The following BLAST results are available for this feature:

BLAST of Carg25767 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011775.1	5.9e-108	100.00	hpxO, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022968891.1	1.4e-104	96.35	monooxygenase 2-like, partial [Cucurbita maxima]	[more]
XP_023554695.1	2.3e-104	96.88	monooxygenase 2-like [Cucurbita pepo subsp. pepo]	[more]
KAG6572121.1	2.3e-104	96.88	Monooxygenase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022953131.1	2.6e-103	95.83	monooxygenase 2-like isoform X1 [Cucurbita moschata] >XP_022953132.1 monooxygena...	[more]

BLAST of Carg25767 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81816	1.1e-53	53.09	Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1	[more]
Q9FLC2	2.8e-52	50.78	Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1	[more]
O81815	1.3e-17	32.39	Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1	[more]
H1ZZA4	1.2e-15	29.67	Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 ...	[more]
Q6F6Y2	2.5e-08	26.88	FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD4...	[more]

BLAST of Carg25767 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HW45	6.5e-105	96.35	monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111468075 PE=3 SV=1	[more]
A0A6J1GMD2	1.2e-103	95.83	monooxygenase 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111455625 PE...	[more]
A0A5A7U5X4	6.8e-86	82.99	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5D3CL17	6.8e-86	82.99	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BIK9	6.8e-86	82.99	FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=...	[more]

BLAST of Carg25767 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38540.1	8.0e-55	53.09	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G05320.1	2.0e-53	50.78	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G29720.1	1.5e-24	34.22	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G35660.1	1.2e-23	34.95	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G35660.2	1.2e-23	34.95	FAD/NAD(P)-binding oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 11..128 e-value: 6.1E-10 score: 38.9
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..189 e-value: 6.9E-27 score: 96.6
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 76..187
IPR044560	Monooxygenase/2-heptyl-3-hydroxy-4(1H)-quinolone synthase	PANTHER	PTHR45934	FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN	coord: 1..191
None	No IPR available	PANTHER	PTHR45934:SF20	MONOOXYGENASE 2-RELATED	coord: 1..191

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25767-RA	Carg25767-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0004497	monooxygenase activity