Carg25727 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25727 vs. NCBI nr
Match: KAG7033560.1 (Gamma-tubulin complex component 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1607.4 bits (4161), Expect = 0.0e+00
Identity = 815/815 (100.00%), Postives = 815/815 (100.00%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
           QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHFLRWEGYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAV 420
           RVYALHFLRWEGYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAV
Sbjct: 361 RVYALHFLRWEGYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAV 420

Query: 421 AAGTTTKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQG 480
           AAGTTTKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQG
Sbjct: 421 AAGTTTKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQG 480

Query: 481 DFVQYLMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTG 540
           DFVQYLMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTG
Sbjct: 481 DFVQYLMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTG 540

Query: 541 DRGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITS 600
           DRGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITS
Sbjct: 541 DRGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITS 600

Query: 601 CSLTKLHHGVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMD 660
           CSLTKLHHGVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMD
Sbjct: 601 CSLTKLHHGVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMD 660

Query: 661 LDDLLAAHEKYLHSIFEKSLLGEQSQPLCKSLFVLFDLILRFRSNADRLYEGIHELQCRT 720
           LDDLLAAHEKYLHSIFEKSLLGEQSQPLCKSLFVLFDLILRFRSNADRLYEGIHELQCRT
Sbjct: 661 LDDLLAAHEKYLHSIFEKSLLGEQSQPLCKSLFVLFDLILRFRSNADRLYEGIHELQCRT 720

Query: 721 IESSLPSRGKSTNIRSTEKSLETASWLADGKKALTQRAREFLRNVEQDLAALAKEYSSLL 780
           IESSLPSRGKSTNIRSTEKSLETASWLADGKKALTQRAREFLRNVEQDLAALAKEYSSLL
Sbjct: 721 IESSLPSRGKSTNIRSTEKSLETASWLADGKKALTQRAREFLRNVEQDLAALAKEYSSLL 780

Query: 781 EGFISQLPLQQHVDLKFLLFRLDFTEFYSQLQPRV 816
           EGFISQLPLQQHVDLKFLLFRLDFTEFYSQLQPRV
Sbjct: 781 EGFISQLPLQQHVDLKFLLFRLDFTEFYSQLQPRV 815

BLAST of Carg25727 vs. NCBI nr
Match: XP_022928720.1 (gamma-tubulin complex component 3 [Cucurbita moschata])

HSP 1 Score: 1571.6 bits (4068), Expect = 0.0e+00
Identity = 809/845 (95.74%), Postives = 810/845 (95.86%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
           QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. NCBI nr
Match: XP_023543538.1 (gamma-tubulin complex component 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1569.3 bits (4062), Expect = 0.0e+00
Identity = 808/845 (95.62%), Postives = 809/845 (95.74%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
           QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPA SISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPATSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. NCBI nr
Match: KAG6603260.1 (Gamma-tubulin complex component 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1568.1 bits (4059), Expect = 0.0e+00
Identity = 807/845 (95.50%), Postives = 808/845 (95.62%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDLIKDLVLRLLSHNP SNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPASNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
           QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLL AHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLTAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. NCBI nr
Match: XP_022967714.1 (gamma-tubulin complex component 3 [Cucurbita maxima])

HSP 1 Score: 1553.9 bits (4022), Expect = 0.0e+00
Identity = 800/845 (94.67%), Postives = 804/845 (95.15%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDL+KDLVLRLLSHNPTSNSS TSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLVKDLVLRLLSHNPTSNSSATSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
            FESSVLLPNLVASDPVLGKKS GAEK+WQKGVLLV+KDPENLRDVAFKEFANLLKEENE
Sbjct: 121 LFESSVLLPNLVASDPVLGKKSGGAEKKWQKGVLLVSKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSN VKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNFVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLD LLAAHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDALLAAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRA EFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAGEFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. ExPASy Swiss-Prot
Match: Q9FG37 (Gamma-tubulin complex component 3 OS=Arabidopsis thaliana OX=3702 GN=GCP3 PE=1 SV=1)

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 614/847 (72.49%), Postives = 698/847 (82.41%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNP-TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDA 60
           ME+DD  K  DL+++LVLRL+S NP T N  P S  F K+LRYA RIL+SR+TPS+ PDA
Sbjct: 1   MEDDDQQKAADLVQELVLRLVSQNPQTPNLDPNSPAFLKTLRYAFRILSSRLTPSVLPDA 60

Query: 61  AAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ 120
            AIAES+KRRLAT+GKSS AL FADLYTKFASKTGPGSVNNKWA++YLLKIV++DRK   
Sbjct: 61  TAIAESLKRRLATQGKSSDALAFADLYTKFASKTGPGSVNNKWALVYLLKIVSDDRKSAI 120

Query: 121 TQFESSVLLPNLVASDPVLGKKSSG--AEKEWQKGVLLVAKDPENLRDVAFKEFANLLKE 180
              +SSVLLPNL   D   G  S G   +K+W  GVLLV+KDPENLRD+AF+E+A L+KE
Sbjct: 121 NGLDSSVLLPNLGIGDTGNGVLSRGEAKKKDWSNGVLLVSKDPENLRDIAFREYAILVKE 180

Query: 181 ENEVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLF 240
           ENEV+EEVLVRDVLYA QGIDGKYVKF++  DGY +   VK PRATR MVR L E+GWLF
Sbjct: 181 ENEVTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLF 240

Query: 241 RKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAAS 300
           RKVK +I+E+M+RFPAEDVGTVG AFCAALQDELS+YYKLLAV+EAQ+MNPIPLVSE+AS
Sbjct: 241 RKVKTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESAS 300

Query: 301 SGNYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRR 360
           S NYLSLRRL+VWFAEPMVKMRLMAVLVDKC+VL+GGAMAGAIHLHAQHGDPLV +FM  
Sbjct: 301 SNNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDFMMS 360

Query: 361 LLRRVYALHF-----------------------------LRW-EGYRLHSGMLPSFISQS 420
           LLR V +  F                             L W EGY+LH  MLPSFIS S
Sbjct: 361 LLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVGQPVKVDLLWREGYKLHPAMLPSFISPS 420

Query: 421 LAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAA 480
           LAQRILRTGKSINFLRVCC+D GWADAA+EAA A+GTTT+RG LGYGETDALE LV  AA
Sbjct: 421 LAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLGYGETDALEHLVTEAA 480

Query: 481 KRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLS 540
           KRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN+IS F+L+
Sbjct: 481 KRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELA 540

Query: 541 GLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 600
           G LE AIR+SNAQYDD D+LDRL+VKMMPHG+GDRGWDVFSLEYEARVPLDTVFTESV+S
Sbjct: 541 GFLEAAIRASNAQYDDRDMLDRLRVKMMPHGSGDRGWDVFSLEYEARVPLDTVFTESVLS 600

Query: 601 KYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVE 660
           KYLR+FNFLWKL+RVEHALIG WKTMKPNCITS S  KL   VKLQLLS LRRCQVLW E
Sbjct: 601 KYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLWNE 660

Query: 661 MNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQP 720
           MNHFV+N QYYIMFEVLEVSWS+FS EMEAA DLDDLLAAHEKYL++I  KSLLGEQSQ 
Sbjct: 661 MNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQSQT 720

Query: 721 LCKSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWL 780
           + +SLFVLF+LILRFRS+ADRLYEGIHELQ R+ ES           R   KS E  SW+
Sbjct: 721 IRESLFVLFELILRFRSHADRLYEGIHELQIRSKESG----------REKNKSQEPGSWI 780

Query: 781 ADGKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEF 815
           ++G+K LTQRA EFL+++ QD+ ++AKEY+S L+GF+S LPLQQ VDLKFL FRLDFTEF
Sbjct: 781 SEGRKGLTQRAGEFLQSMSQDMDSIAKEYTSSLDGFLSLLPLQQSVDLKFLFFRLDFTEF 837

BLAST of Carg25727 vs. ExPASy Swiss-Prot
Match: O73787 (Gamma-tubulin complex component 3 homolog OS=Xenopus laevis OX=8355 GN=tubgcp3 PE=1 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 1.2e-117
Identity = 285/911 (31.28%), Postives = 444/911 (48.74%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L            +D  +  +YA+R++ S   P++  D   + E IK+   
Sbjct: 12  LLQNLCCRILGKG--------EADVAQQFQYAVRVIGSNFAPTVERDEFLVTEKIKKEFV 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQTQFES-----SV 131
            + + +    F++L+ K  S+   G + N+W++LYLL  ++ED + +  +  S     + 
Sbjct: 72  RQRREADGALFSELHRKLQSQ---GVLKNRWSILYLLLSLSEDPRKQPNKTSSFAALFAQ 131

Query: 132 LLPNLVASDPVLGKKSSGAEKEWQ---------------KGVLLV--------------- 191
            LP    S P    +       +Q                G+  +               
Sbjct: 132 ALPRDAHSTPYYYARPQSLPLSYQDRNVQCAQNAASIGSSGISSIGMYALNGPTPQSIIQ 191

Query: 192 --AKDPENLRDVAFKEFANLL-------------------------------KEEN---- 251
             +    N+ D   ++  + L                               + E     
Sbjct: 192 GQSNQTPNMGDALRQQLGSRLAWTLAAGQQPSQQSTTTKGLPNTVSRNVPRTRREGDSSG 251

Query: 252 --EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLF 311
             E++E  LVRD+LY  QGIDGK+VK  N+ + Y +   V   ++ + +  KL E+GWL 
Sbjct: 252 SVEITETSLVRDLLYVFQGIDGKFVKMCNSENCYKVDGKVAVSKSLKDITSKLSELGWLH 311

Query: 312 RKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQ-SMNPIPLVSEAA 371
            K+K Y     +R      G VG +FCAAL  EL EYY+LL+V+ +Q  +     V+   
Sbjct: 312 NKIKKYTD---QRSLDRAFGLVGQSFCAALHQELKEYYRLLSVLHSQLQVEDDQGVNLGV 371

Query: 372 SSGNYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMR 431
            S   L+LRRL VW  +P ++++ +A LVD C+  KGG +A A+H + + GDP +   ++
Sbjct: 372 ESS--LTLRRLLVWTFDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLVQ 431

Query: 432 RLLRRVY--ALHFL-RW----------------------------EGYRLHSGMLPSFIS 491
            +L  V    L+FL RW                            + Y L   M+PSF++
Sbjct: 432 HILGLVAYPILNFLYRWIYDGELEDTYHEFFVASDPVVKTDRLWHDKYSLRKSMIPSFMT 491

Query: 492 QSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDG 551
              ++++L  GKSINFL   C D   A  A     +A +      L     +A ++ +D 
Sbjct: 492 MDQSRKVLLIGKSINFLHQVCHDQTPASKAMAVGKSAESPKDAAELFTDLENAFQTKIDA 551

Query: 552 AAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFK 611
           A     K+LLDV++K Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA ++    
Sbjct: 552 AYFDTSKYLLDVLNKNYNLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQHN 611

Query: 612 LSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESV 671
           L+G+LETA+R++NAQ+D+P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT   
Sbjct: 612 LTGILETAVRATNAQFDNPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTREC 671

Query: 672 MSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLW 731
           MS YLR+FNFLW+ +R+E+ L   WK    N        KL  G+  +L   L +C +L 
Sbjct: 672 MSHYLRVFNFLWRAKRMEYILTDIWKGHMCN-------AKLLKGMP-ELSGVLHQCHILA 731

Query: 732 VEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQS 791
            EM HF+  +QYYI FEVLE SW +  N++  A DLD ++AAH+ +L +I  + LL  +S
Sbjct: 732 SEMVHFIHQMQYYITFEVLECSWDELWNKVLKAQDLDHIIAAHDVFLDTIISRCLLDSES 791

Query: 792 QPLCKSLFVLFDLILRFRSNADRLYE-GIHELQCRTIESSLPSRGKSTNIRSTEKSLETA 816
           + L   L  +FD I+ F++  D LY   + ELQ R        R K    R +E      
Sbjct: 792 RALLNQLRAVFDQIIEFQNAQDALYRAALEELQQRL---QFEERKKE---RESEGEWGVT 851

BLAST of Carg25727 vs. ExPASy Swiss-Prot
Match: Q96CW5 (Gamma-tubulin complex component 3 OS=Homo sapiens OX=9606 GN=TUBGCP3 PE=1 SV=2)

HSP 1 Score: 417.2 bits (1071), Expect = 4.4e-115
Identity = 283/917 (30.86%), Postives = 437/917 (47.66%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L          + +D  +  +YA+R++ S   P++  D   +AE IK+ L 
Sbjct: 12  LLQNLCCRILGR--------SEADVAQQFQYAVRVIGSNFAPTVERDEFLVAEKIKKELI 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAE------------------ 131
            + + + A  F++L+ K  S+   G + NKW++LYLL  ++E                  
Sbjct: 72  RQRREADAALFSELHRKLHSQ---GVLKNKWSILYLLLSLSEDPRRQPSKVSSYATLFAQ 131

Query: 132 ------------------------DRKCRQTQFESSV----------------------L 191
                                   DR  +  Q   SV                      L
Sbjct: 132 ALPRDAHSTPYYYARPQTLPLSYQDRSAQSAQSSGSVGSSGISSIGLCALSGPAPAPQSL 191

Query: 192 LPNLVASDPVLG---KKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN----- 251
           LP      P +G   ++  G+   W       +      + V      N+ +        
Sbjct: 192 LPGQSNQAPGVGDCLRQQLGSRLAWTLTANQPSSQATTSKGVPSAVSRNMTRSRREGDTG 251

Query: 252 ---EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWL 311
              E++E  LVRD+LY  QGIDGK +K +N  + Y +       R+ R    +L E+GWL
Sbjct: 252 GTMEITEAALVRDILYVFQGIDGKNIKMNNTENCYKVEGKANLSRSLRDTAVRLSELGWL 311

Query: 312 FRKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQ-SMNPIPLVSEA 371
             K++ Y     +R      G VG +FCAAL  EL EYY+LL+V+ +Q  +     V+  
Sbjct: 312 HNKIRRYTD---QRSLDRSFGLVGQSFCAALHQELREYYRLLSVLHSQLQLEDDQGVNLG 371

Query: 372 ASSGNYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFM 431
             S   L+LRRL VW  +P ++++ +A LVD C+  KGG +A A+H + + GDP +   +
Sbjct: 372 LESS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMRSLV 431

Query: 432 RRLLRRVY--ALHFL-RW----------------------------EGYRLHSGMLPSFI 491
           + +L  V    L FL RW                            + Y L   M+PSF+
Sbjct: 432 QHILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIPSFM 491

Query: 492 SQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVD 551
           +   ++++L  GKSINFL   C D            +A +      L     +A +  +D
Sbjct: 492 TMDQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPQDAADLFTDLENAFQGKID 551

Query: 552 GAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLF 611
            A     K+LLDV++K+Y   DH  A++RYLLLGQGDF+++LMD++ PEL  PA ++   
Sbjct: 552 AAYFETSKYLLDVLNKKYSLLDHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTLYQH 611

Query: 612 KLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTES 671
            L+G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVFT  
Sbjct: 612 NLTGILETAVRATNAQFDSPEILRRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVFTRE 671

Query: 672 VMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVL 731
            MS YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C +L
Sbjct: 672 CMSHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQCHIL 731

Query: 732 WVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQ 791
             EM HF+  +QYYI FEVLE SW +  N+++ A DLD ++AAHE +L +I  + LL   
Sbjct: 732 ASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHEVFLDTIISRCLLDSD 791

Query: 792 SQPLCKSLFVLFDLILRFRSNADRLYE-GIHELQCRTIESSLPSRGKSTNIRSTEKSLET 816
           S+ L   L  +FD I+  ++  D +Y   + ELQ          R      +  ++ +E 
Sbjct: 792 SRALLNQLRAVFDQIIELQNAQDAIYRAALEELQ----------RRLQFEEKKKQREIE- 851

BLAST of Carg25727 vs. ExPASy Swiss-Prot
Match: P58854 (Gamma-tubulin complex component 3 OS=Mus musculus OX=10090 GN=Tubgcp3 PE=1 SV=2)

HSP 1 Score: 410.6 bits (1054), Expect = 4.1e-113
Identity = 286/920 (31.09%), Postives = 439/920 (47.72%), Query Frame = 0

Query: 12  LIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAAAIAESIKRRLA 71
           L+++L  R+L          + +D  +  ++A+R++ S   P++  D   +AE IK+ L 
Sbjct: 12  LLQNLCCRILGR--------SEADVAQQFQFAVRVIGSNFAPTVERDEFLVAEKIKKELI 71

Query: 72  TEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRK--------------- 131
            + + + A  F++L+ K  S+   G + NKW++LYLL  ++ED +               
Sbjct: 72  RQRREADAALFSELHRKLHSQ---GVLKNKWSILYLLLNLSEDPRKQASKVTSYASLFAQ 131

Query: 132 ------------------------CRQTQFESS-----------------------VLLP 191
                                    R TQ +SS                         LP
Sbjct: 132 ALPRDAHSTPYYYARPQTLPLNYQDRSTQAQSSGSLGSSGISSIGMCGLSGPTPVQPFLP 191

Query: 192 NLVASDPVLG---KKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEE-------- 251
                 P +G   ++  G    W     L    P +    + K F N L           
Sbjct: 192 GQSHQAPGVGDGLRQQLGPRLAW----TLTGNQPSSQTPTS-KGFPNALSRNLTRSRREG 251

Query: 252 -----NEVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEI 311
                 EV+E  LVRD+LY  QGIDGK +K  +  + Y +       ++ R    +L E+
Sbjct: 252 DPGGTLEVTEAALVRDILYVFQGIDGKNIKMSSTENCYKVEAKANLNKSLRDTAVRLAEL 311

Query: 312 GWLFRKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQ-SMNPIPLV 371
           GWL  K++ Y     +R      G VG +FCAAL  EL EYY+LL+V+ +Q  +     V
Sbjct: 312 GWLHNKIRKYAD---QRSLDRSFGLVGQSFCAALHQELKEYYRLLSVLHSQLQLEDDQGV 371

Query: 372 SEAASSGNYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVL 431
           +    S   L+LRRL VW  +P ++++ +A LVD C+  KGG +A A+H + + GDP + 
Sbjct: 372 NLGLESS--LTLRRLLVWTYDPKIRLKTLAALVDHCQGRKGGELASAVHAYTKTGDPYMK 431

Query: 432 EFMRRLLRRVY--ALHFL-RW----------------------------EGYRLHSGMLP 491
             ++ +L  V    L FL RW                            + Y L   M+P
Sbjct: 432 SLVQHILSLVSHPVLSFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYTLRKSMIP 491

Query: 492 SFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALES 551
           SFI+   ++++L  GKSINFL   C D            +A +      L     +A + 
Sbjct: 492 SFITMDQSRKVLLIGKSINFLHQVCHDQTPTTKMIAVTKSAESPRDAADLFTDLENAFQG 551

Query: 552 LVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSI 611
            +D A     K+LLDV++K+Y   +H  A++RYLLLGQGDF+++LMD++ PEL  PA ++
Sbjct: 552 KIDAAYFETSKYLLDVLNKKYSLLEHMQAMRRYLLLGQGDFIRHLMDLLKPELVRPATTL 611

Query: 612 SLFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVF 671
               L+G+LETA+R++NAQ+D P+IL RL V+++    GD GWDVFSL+Y    P+ TVF
Sbjct: 612 YQHNLTGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVDGPIATVF 671

Query: 672 TESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRC 731
           T   MS YLR+FNFLW+ +R+E+ L    K    N        KL   +  +    L +C
Sbjct: 672 TRECMSHYLRVFNFLWRAKRMEYILTDIRKGHMCN-------AKLLRNMP-EFSGVLHQC 731

Query: 732 QVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLL 791
            +L  EM HF+  +QYYI FEVLE SW +  N ++ A DLD ++AAHE +L++I  + LL
Sbjct: 732 HILASEMVHFIHQMQYYITFEVLECSWDELWNRVQQAQDLDHIIAAHEAFLNTITSRCLL 791

Query: 792 GEQSQPLCKSLFVLFDLILRFRSNADRLYE-GIHELQCRTIESSLPSRGKSTNIRSTEKS 816
              S+ L   L  +FD I+  ++  D +Y   + ELQ          R      +  ++ 
Sbjct: 792 DSNSRVLLNQLRAVFDQIIELQNAQDVMYRAALEELQ----------RRLQFEEKKKQRE 851

BLAST of Carg25727 vs. ExPASy Swiss-Prot
Match: Q95ZG4 (Spindle pole body component 98 OS=Dictyostelium discoideum OX=44689 GN=spc98 PE=1 SV=2)

HSP 1 Score: 309.7 bits (792), Expect = 9.9e-83
Identity = 214/750 (28.53%), Postives = 366/750 (48.80%), Query Frame = 0

Query: 179 NEVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVL-----SNIVKAPRA-----TRTMVR 238
           NE+ E +L+RD++Y  QGIDG Y+K++  +D + +     + +V    A      R +V 
Sbjct: 102 NEIPEHLLIRDIIYVFQGIDGTYIKYNKQSDSFKIDENTSNTLVNGEPAYISKPKRDLVY 161

Query: 239 KLCEIGWLFRKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNP 298
           +LCE GWLF+KV+ +I+ N      +  G    +FC+A+ DEL E Y+++A++E Q    
Sbjct: 162 RLCEFGWLFKKVRLFITNN----DFKKTGLTNQSFCSAINDELIELYRIIAILETQVYKK 221

Query: 299 IPLVS-----------------EAASS----------------GNYLSLRRLAVWFAEPM 358
             +V+                 E+ SS                G+ L+L RL VW   P+
Sbjct: 222 FDMVNYGGGGGGSGGSGSGSGLESPSSVSSGGTTTSTEIPFIDGDSLTLIRLFVWIQSPL 281

Query: 359 VKMRLMAVLVDKCRV-LKGGAMAGAIHLHAQHGDPLVLEFMRRLLRRV------------ 418
            +++++   VD   V +KGG +   I   ++HGD  +   +  ++ ++            
Sbjct: 282 KRLKVLGTCVDSITVDMKGGEILSKIDTLSKHGDQDIRILIHNIMFKICQPLFSMIRLWM 341

Query: 419 --------YALHFLR----------W-EGYRLHSGMLPSFISQSLAQRILRTGKSINFLR 478
                   Y   F+R          W E + + + +LPSFIS  L++RIL  GKSIN+++
Sbjct: 342 FKGEINDPYQEFFIRQYESVQLEKTWKEKFAIVARLLPSFISLPLSKRILIIGKSINYMK 401

Query: 479 VCC-----------------------------------EDMGWADAATEAAVAAGTT--- 538
             C                                   E+ G  D   E  +    +   
Sbjct: 402 QFCNNFKEDKNDRYYYYNQEDDDDDDEDHDDNDDDDENENQGEDDEIIERKLLIKESKII 461

Query: 539 -TKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQ 598
             K   L Y   + L+ +++  +++  + LL ++  R+KF +H  A+K+YLLLGQGDF+Q
Sbjct: 462 KEKTKELNYINKEVLQEIIELVSRQSSERLLKIVLNRFKFMNHVKALKKYLLLGQGDFIQ 521

Query: 599 YLMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGW 658
           YLMD++G +L +P + I   KL G ++TAIR+SNAQ+++ DI++RL + ++P   G+ GW
Sbjct: 522 YLMDLIGEDLLKPTSQIQRHKLVGWMDTAIRNSNAQFEEQDIVNRLDIALLPERPGNIGW 581

Query: 659 DVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLT 718
           D+FSL+Y    PL+T+ + + + +Y +IF+F+W ++RVE++L   W+ ++ +  TS S+ 
Sbjct: 582 DIFSLDYHVDTPLNTILSPNDILRYKKIFHFMWGIKRVEYSLASIWRKIRSS--TSLSIL 641

Query: 719 KLHHGVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEM-EAAMDLDD 778
               G        + +  ++  EM HF+SN QYY+MFEVLE SW +    + + A DLD 
Sbjct: 642 SPIGG-------DIHKSHLIMNEMVHFISNFQYYLMFEVLECSWKNLEKFIDQEATDLDQ 701

Query: 779 LLAAHEKYLHSIFEKSLLGEQSQPLCKSLFVLFDLILRFRSNADRLYEGIHELQCRTIES 811
           L+ AH +YL             Q +C  +F+         SN+D  YE   +L    I+ 
Sbjct: 702 LIEAHHQYL-------------QDICNKMFL---------SNSDSCYECFKKLLSIIIKF 761

BLAST of Carg25727 vs. ExPASy TrEMBL
Match: A0A6J1ESD9 (Gamma-tubulin complex component OS=Cucurbita moschata OX=3662 GN=LOC111435547 PE=3 SV=1)

HSP 1 Score: 1571.6 bits (4068), Expect = 0.0e+00
Identity = 809/845 (95.74%), Postives = 810/845 (95.86%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
           QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE
Sbjct: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. ExPASy TrEMBL
Match: A0A6J1HXI7 (Gamma-tubulin complex component OS=Cucurbita maxima OX=3661 GN=LOC111467159 PE=3 SV=1)

HSP 1 Score: 1553.9 bits (4022), Expect = 0.0e+00
Identity = 800/845 (94.67%), Postives = 804/845 (95.15%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDDPSKVLDL+KDLVLRLLSHNPTSNSS TSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDPSKVLDLVKDLVLRLLSHNPTSNSSATSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGAEKEWQKGVLLVAKDPENLRDVAFKEFANLLKEENE 180
            FESSVLLPNLVASDPVLGKKS GAEK+WQKGVLLV+KDPENLRDVAFKEFANLLKEENE
Sbjct: 121 LFESSVLLPNLVASDPVLGKKSGGAEKKWQKGVLLVSKDPENLRDVAFKEFANLLKEENE 180

Query: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRKV 240
           VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSN VKAPRATRTMVRKLCEIGWLFRKV
Sbjct: 181 VSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNFVKAPRATRTMVRKLCEIGWLFRKV 240

Query: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300
           KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN
Sbjct: 241 KGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSGN 300

Query: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360
           YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR
Sbjct: 301 YLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLLR 360

Query: 361 RVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLAQ 420
           RV +  F                              L  EGYRLHSGMLPSFISQSLAQ
Sbjct: 361 RVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHSGMLPSFISQSLAQ 420

Query: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480
           RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI
Sbjct: 421 RILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKRI 480

Query: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540
           DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL
Sbjct: 481 DKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGLL 540

Query: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600
           ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL
Sbjct: 541 ETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKYL 600

Query: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660
           RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH
Sbjct: 601 RIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMNH 660

Query: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCK 720
           FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLD LLAAHEKYLHSIFEKSLLGEQSQPLCK
Sbjct: 661 FVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDALLAAHEKYLHSIFEKSLLGEQSQPLCK 720

Query: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780
           SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG
Sbjct: 721 SLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLADG 780

Query: 781 KKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 816
           KKALTQRA EFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ
Sbjct: 781 KKALTQRAGEFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYSQ 840

BLAST of Carg25727 vs. ExPASy TrEMBL
Match: A0A0A0KYU4 (Gamma-tubulin complex component OS=Cucumis sativus OX=3659 GN=Csa_4G561690 PE=3 SV=1)

HSP 1 Score: 1502.6 bits (3889), Expect = 0.0e+00
Identity = 771/846 (91.13%), Postives = 792/846 (93.62%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDD +KVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGA-EKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180
           QFESS+LLPNLVASDPVLGKKS  A EKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSMLLPNLVASDPVLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRK 240
           EV+EEVLVRDVLYACQGIDGKYVKFDNN+DGYVLSN+VKA RATRTMVRKLCE+GWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSG 300
           VKGYISE+MERFPAED+GTVGHAFCAALQDELSEYYKLLA++EAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPM KMRLMAVLVDKCRVLKGG MAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGTMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLA 420
           RRV +  F                              L  EGYRLH+GMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRG LGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPAN+IS FKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLC 720
           HFV+NLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ LC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLAD 780
           KSLFVLFD+ILRFRS+ADRLYEGIHELQCRTIESSLPSR KS   R+TE+SLETASW+AD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKSRTTERSLETASWIAD 780

Query: 781 GKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYS 816
           GKKALTQRA EFLRNVEQDLAALAKEYSSLLE FISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

BLAST of Carg25727 vs. ExPASy TrEMBL
Match: A0A5A7THP1 (Gamma-tubulin complex component OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold128G00670 PE=3 SV=1)

HSP 1 Score: 1499.2 bits (3880), Expect = 0.0e+00
Identity = 769/846 (90.90%), Postives = 793/846 (93.74%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDD +KVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGA-EKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180
           QFESS+LLP+LVASDP+LGKKS  A EKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRK 240
           EV+EEVLVRDVLYACQGIDGKYVKFDNN+DGYVLSN+VKA RATRTMVRKLCE+GWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSG 300
           VKGYISE+MERFPAED+GTVGHAFCAALQDELSEYYKLLA++EAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPM KMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLA 420
           RRV +  F                              L  EGYRLH+GMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRG LGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN+IS FKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLC 720
           HFV+NLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ LC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLAD 780
           KSLFVLFD+ILRFRS+ADRLYEGIHELQCRTIESSLPSR KS   R+TEKSLETASW+AD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYS 816
           GKKALTQRA EFLRNVEQDLAALAKEYSSLLE FISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

BLAST of Carg25727 vs. ExPASy TrEMBL
Match: A0A1S3B579 (Gamma-tubulin complex component OS=Cucumis melo OX=3656 GN=LOC103486142 PE=3 SV=1)

HSP 1 Score: 1499.2 bits (3880), Expect = 0.0e+00
Identity = 769/846 (90.90%), Postives = 793/846 (93.74%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60
           MEEDD +KVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA
Sbjct: 1   MEEDDSTKVLDLIKDLVLRLLSHNPTSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDAA 60

Query: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQT 120
           AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ+
Sbjct: 61  AIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQS 120

Query: 121 QFESSVLLPNLVASDPVLGKKSSGA-EKEWQKGVLLVAKDPENLRDVAFKEFANLLKEEN 180
           QFESS+LLP+LVASDP+LGKKS  A EKEWQKGVLLVAKDPENLRDVAFKEF+NLLKEEN
Sbjct: 121 QFESSMLLPSLVASDPLLGKKSGAAPEKEWQKGVLLVAKDPENLRDVAFKEFSNLLKEEN 180

Query: 181 EVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLFRK 240
           EV+EEVLVRDVLYACQGIDGKYVKFDNN+DGYVLSN+VKA RATRTMVRKLCE+GWLFRK
Sbjct: 181 EVTEEVLVRDVLYACQGIDGKYVKFDNNSDGYVLSNLVKASRATRTMVRKLCEMGWLFRK 240

Query: 241 VKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAASSG 300
           VKGYISE+MERFPAED+GTVGHAFCAALQDELSEYYKLLA++EAQSMNPIPLVSEAASSG
Sbjct: 241 VKGYISESMERFPAEDIGTVGHAFCAALQDELSEYYKLLAILEAQSMNPIPLVSEAASSG 300

Query: 301 NYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360
           NYLSLRRLAVWFAEPM KMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL
Sbjct: 301 NYLSLRRLAVWFAEPMAKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRRLL 360

Query: 361 RRVYALHF------------------------------LRWEGYRLHSGMLPSFISQSLA 420
           RRV +  F                              L  EGYRLH+GMLPSFISQSLA
Sbjct: 361 RRVCSPLFEMVRSWVLEGELEDIFSEFFVVGQQVKAESLWREGYRLHAGMLPSFISQSLA 420

Query: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAAKR 480
           QRILRTGKSINFLRVCCEDMGWADAATEAA AAGTTTKRG LGYGETDALESLVD AAKR
Sbjct: 421 QRILRTGKSINFLRVCCEDMGWADAATEAAAAAGTTTKRGGLGYGETDALESLVDEAAKR 480

Query: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 540
           IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN+IS FKLSGL
Sbjct: 481 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPDLSEPANAISSFKLSGL 540

Query: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600
           LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY
Sbjct: 541 LETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMSKY 600

Query: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660
           LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN
Sbjct: 601 LRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVEMN 660

Query: 661 HFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQPLC 720
           HFV+NLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQ LC
Sbjct: 661 HFVTNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQTLC 720

Query: 721 KSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWLAD 780
           KSLFVLFD+ILRFRS+ADRLYEGIHELQCRTIESSLPSR KS   R+TEKSLETASW+AD
Sbjct: 721 KSLFVLFDIILRFRSHADRLYEGIHELQCRTIESSLPSRDKSKKNRTTEKSLETASWIAD 780

Query: 781 GKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEFYS 816
           GKKALTQRA EFLRNVEQDLAALAKEYSSLLE FISQLPLQQHVDLKFLLFRLDFTEFYS
Sbjct: 781 GKKALTQRAGEFLRNVEQDLAALAKEYSSLLEEFISQLPLQQHVDLKFLLFRLDFTEFYS 840

BLAST of Carg25727 vs. TAIR 10
Match: AT5G06680.1 (spindle pole body component 98 )

HSP 1 Score: 1187.6 bits (3071), Expect = 0.0e+00
Identity = 614/847 (72.49%), Postives = 698/847 (82.41%), Query Frame = 0

Query: 1   MEEDDPSKVLDLIKDLVLRLLSHNP-TSNSSPTSSDFQKSLRYAIRILTSRMTPSIAPDA 60
           ME+DD  K  DL+++LVLRL+S NP T N  P S  F K+LRYA RIL+SR+TPS+ PDA
Sbjct: 1   MEDDDQQKAADLVQELVLRLVSQNPQTPNLDPNSPAFLKTLRYAFRILSSRLTPSVLPDA 60

Query: 61  AAIAESIKRRLATEGKSSQALTFADLYTKFASKTGPGSVNNKWAVLYLLKIVAEDRKCRQ 120
            AIAES+KRRLAT+GKSS AL FADLYTKFASKTGPGSVNNKWA++YLLKIV++DRK   
Sbjct: 61  TAIAESLKRRLATQGKSSDALAFADLYTKFASKTGPGSVNNKWALVYLLKIVSDDRKSAI 120

Query: 121 TQFESSVLLPNLVASDPVLGKKSSG--AEKEWQKGVLLVAKDPENLRDVAFKEFANLLKE 180
              +SSVLLPNL   D   G  S G   +K+W  GVLLV+KDPENLRD+AF+E+A L+KE
Sbjct: 121 NGLDSSVLLPNLGIGDTGNGVLSRGEAKKKDWSNGVLLVSKDPENLRDIAFREYAILVKE 180

Query: 181 ENEVSEEVLVRDVLYACQGIDGKYVKFDNNADGYVLSNIVKAPRATRTMVRKLCEIGWLF 240
           ENEV+EEVLVRDVLYA QGIDGKYVKF++  DGY +   VK PRATR MVR L E+GWLF
Sbjct: 181 ENEVTEEVLVRDVLYASQGIDGKYVKFNSEIDGYAVQESVKVPRATRIMVRMLSELGWLF 240

Query: 241 RKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSEAAS 300
           RKVK +I+E+M+RFPAEDVGTVG AFCAALQDELS+YYKLLAV+EAQ+MNPIPLVSE+AS
Sbjct: 241 RKVKTFITESMDRFPAEDVGTVGQAFCAALQDELSDYYKLLAVLEAQAMNPIPLVSESAS 300

Query: 301 SGNYLSLRRLAVWFAEPMVKMRLMAVLVDKCRVLKGGAMAGAIHLHAQHGDPLVLEFMRR 360
           S NYLSLRRL+VWFAEPMVKMRLMAVLVDKC+VL+GGAMAGAIHLHAQHGDPLV +FM  
Sbjct: 301 SNNYLSLRRLSVWFAEPMVKMRLMAVLVDKCKVLRGGAMAGAIHLHAQHGDPLVHDFMMS 360

Query: 361 LLRRVYALHF-----------------------------LRW-EGYRLHSGMLPSFISQS 420
           LLR V +  F                             L W EGY+LH  MLPSFIS S
Sbjct: 361 LLRCVCSPLFEMVRSWVLEGELEDTFGEFFVVGQPVKVDLLWREGYKLHPAMLPSFISPS 420

Query: 421 LAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRGSLGYGETDALESLVDGAA 480
           LAQRILRTGKSINFLRVCC+D GWADAA+EAA A+GTTT+RG LGYGETDALE LV  AA
Sbjct: 421 LAQRILRTGKSINFLRVCCDDHGWADAASEAAAASGTTTRRGGLGYGETDALEHLVTEAA 480

Query: 481 KRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLS 540
           KRIDKHLLDV++KRYKFK+HCLAIKRYLLLGQGDFVQYLMDIVGP+LSEPAN+IS F+L+
Sbjct: 481 KRIDKHLLDVLYKRYKFKEHCLAIKRYLLLGQGDFVQYLMDIVGPKLSEPANNISSFELA 540

Query: 541 GLLETAIRSSNAQYDDPDILDRLKVKMMPHGTGDRGWDVFSLEYEARVPLDTVFTESVMS 600
           G LE AIR+SNAQYDD D+LDRL+VKMMPHG+GDRGWDVFSLEYEARVPLDTVFTESV+S
Sbjct: 541 GFLEAAIRASNAQYDDRDMLDRLRVKMMPHGSGDRGWDVFSLEYEARVPLDTVFTESVLS 600

Query: 601 KYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHHGVKLQLLSTLRRCQVLWVE 660
           KYLR+FNFLWKL+RVEHALIG WKTMKPNCITS S  KL   VKLQLLS LRRCQVLW E
Sbjct: 601 KYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNSFVKLQSSVKLQLLSALRRCQVLWNE 660

Query: 661 MNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAHEKYLHSIFEKSLLGEQSQP 720
           MNHFV+N QYYIMFEVLEVSWS+FS EMEAA DLDDLLAAHEKYL++I  KSLLGEQSQ 
Sbjct: 661 MNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLDDLLAAHEKYLNAIVGKSLLGEQSQT 720

Query: 721 LCKSLFVLFDLILRFRSNADRLYEGIHELQCRTIESSLPSRGKSTNIRSTEKSLETASWL 780
           + +SLFVLF+LILRFRS+ADRLYEGIHELQ R+ ES           R   KS E  SW+
Sbjct: 721 IRESLFVLFELILRFRSHADRLYEGIHELQIRSKESG----------REKNKSQEPGSWI 780

Query: 781 ADGKKALTQRAREFLRNVEQDLAALAKEYSSLLEGFISQLPLQQHVDLKFLLFRLDFTEF 815
           ++G+K LTQRA EFL+++ QD+ ++AKEY+S L+GF+S LPLQQ VDLKFL FRLDFTEF
Sbjct: 781 SEGRKGLTQRAGEFLQSMSQDMDSIAKEYTSSLDGFLSLLPLQQSVDLKFLFFRLDFTEF 837

BLAST of Carg25727 vs. TAIR 10
Match: AT5G17410.2 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 127.9 bits (320), Expect = 3.8e-29
Identity = 138/575 (24.00%), Postives = 237/575 (41.22%), Query Frame = 0

Query: 178 ENEVSEEVLVRDVLYACQGIDGKYV---KFDNNADGYVLSNIVKAPRATRTMVRKLCEIG 237
           +  V E +++ D+L A  GI+G+Y+   +F    D            A + + +++  + 
Sbjct: 60  DTPVQELIVIDDLLSALVGIEGRYISIKRFHGKEDSIAFQVDPSMDLALQELAKRIFPLC 119

Query: 238 WLFRKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSE 297
             +  +  ++ E+  +F     G V HAF AAL+  L +Y  ++A +E Q          
Sbjct: 120 EYYLLIDQFV-ESSSQFKN---GLVNHAFAAALRALLLDYQAMVAQLEHQ---------- 179

Query: 298 AASSGNYLSLRRLAV---WF-AEPMV-KMRLMAVLVDKCRVLKGGAMAGAIHL-----HA 357
                    L RL++   WF  +PM+  MR +A ++ +    K    +G ++L      A
Sbjct: 180 -------FRLGRLSIQGLWFYCQPMMGSMRALAAVIQQAST-KQFVGSGVLNLLQSQAKA 239

Query: 358 QHGDPLV---LEFMRRLLRRVYALHFLRW------------------------------- 417
             GD  V   LE M       Y     RW                               
Sbjct: 240 MAGDNSVRSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIAENRSLKKESLSQDST 299

Query: 418 -----EGYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTT 477
                + Y L    +P F++ ++A  IL TGK +N +R C  +           V    +
Sbjct: 300 AKYWSQRYSL-KDTIPGFLA-NIAATILTTGKYLNVMRECGHN-----------VQVPIS 359

Query: 478 TKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQY 537
            +     +G        +  A +     L++++  +Y       +IK YLLL QGDF+ +
Sbjct: 360 ERSKLTIFGSNHHYLECIKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVH 419

Query: 538 LMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPD-----ILDR--LKVKMMPHG 597
            MDI   EL++  + IS+ KL  LL+ A+R++ A  D         +DR  L   +  H 
Sbjct: 420 FMDIAREELNKKVHEISVEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHK 479

Query: 598 TGD----------RGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIG 657
             D           G + FSL Y+ + PL  V ++  +SKY  IF FL+  + VE  L G
Sbjct: 480 DTDSNSIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCG 539

Query: 658 TWKTMKPNCITSCSLTKLHHGVKLQLL--STLRRCQVLWVEMNHFVSNLQYYIMFEVLEV 682
            W              ++H G++      + + R  +L   M  F+S+L +Y+ FEVLE 
Sbjct: 540 AW--------------QIHQGIRSMNSKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEP 585

BLAST of Carg25727 vs. TAIR 10
Match: AT5G17410.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 127.9 bits (320), Expect = 3.8e-29
Identity = 138/575 (24.00%), Postives = 237/575 (41.22%), Query Frame = 0

Query: 178 ENEVSEEVLVRDVLYACQGIDGKYV---KFDNNADGYVLSNIVKAPRATRTMVRKLCEIG 237
           +  V E +++ D+L A  GI+G+Y+   +F    D            A + + +++  + 
Sbjct: 59  DTPVQELIVIDDLLSALVGIEGRYISIKRFHGKEDSIAFQVDPSMDLALQELAKRIFPLC 118

Query: 238 WLFRKVKGYISENMERFPAEDVGTVGHAFCAALQDELSEYYKLLAVVEAQSMNPIPLVSE 297
             +  +  ++ E+  +F     G V HAF AAL+  L +Y  ++A +E Q          
Sbjct: 119 EYYLLIDQFV-ESSSQFKN---GLVNHAFAAALRALLLDYQAMVAQLEHQ---------- 178

Query: 298 AASSGNYLSLRRLAV---WF-AEPMV-KMRLMAVLVDKCRVLKGGAMAGAIHL-----HA 357
                    L RL++   WF  +PM+  MR +A ++ +    K    +G ++L      A
Sbjct: 179 -------FRLGRLSIQGLWFYCQPMMGSMRALAAVIQQAST-KQFVGSGVLNLLQSQAKA 238

Query: 358 QHGDPLV---LEFMRRLLRRVYALHFLRW------------------------------- 417
             GD  V   LE M       Y     RW                               
Sbjct: 239 MAGDNSVRSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIAENRSLKKESLSQDST 298

Query: 418 -----EGYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTT 477
                + Y L    +P F++ ++A  IL TGK +N +R C  +           V    +
Sbjct: 299 AKYWSQRYSL-KDTIPGFLA-NIAATILTTGKYLNVMRECGHN-----------VQVPIS 358

Query: 478 TKRGSLGYGETDALESLVDGAAKRIDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQY 537
            +     +G        +  A +     L++++  +Y       +IK YLLL QGDF+ +
Sbjct: 359 ERSKLTIFGSNHHYLECIKAAHEFASIELVNLIKDKYDLVGRLRSIKHYLLLDQGDFLVH 418

Query: 538 LMDIVGPELSEPANSISLFKLSGLLETAIRSSNAQYDDPD-----ILDR--LKVKMMPHG 597
            MDI   EL++  + IS+ KL  LL+ A+R++ A  D         +DR  L   +  H 
Sbjct: 419 FMDIAREELNKKVHEISVEKLQSLLDLALRTTAAAADPRHEDLTCCVDRASLLTTLGMHK 478

Query: 598 TGD----------RGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIG 657
             D           G + FSL Y+ + PL  V ++  +SKY  IF FL+  + VE  L G
Sbjct: 479 DTDSNSIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCG 538

Query: 658 TWKTMKPNCITSCSLTKLHHGVKLQLL--STLRRCQVLWVEMNHFVSNLQYYIMFEVLEV 682
            W              ++H G++      + + R  +L   M  F+S+L +Y+ FEVLE 
Sbjct: 539 AW--------------QIHQGIRSMNSKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEP 584

BLAST of Carg25727 vs. TAIR 10
Match: AT3G53760.1 (GAMMA-TUBULIN COMPLEX PROTEIN 4 )

HSP 1 Score: 89.0 bits (219), Expect = 2.0e-17
Identity = 112/467 (23.98%), Postives = 178/467 (38.12%), Query Frame = 0

Query: 372 GYRLHSGMLPSFISQSLAQRILRTGKSINFLRVCCEDMGWADAATEAAVAAGTTTKRG-- 431
           G+ +   MLP +I   L + IL  GK+I  LR       +    +      G+   RG  
Sbjct: 270 GFHISLDMLPDYIPMRLGESILFAGKAIRVLRNPSPAFQFQKDKSFQQTMRGSQRIRGFM 329

Query: 432 -------------------SLGYGETDALESL------------------VDGAAKRIDK 491
                               L   E D +E++                  VD        
Sbjct: 330 HSDFPETETELDADLTGGELLPQSEADKIEAMLKDLKESSEFHKRSFECTVDSVRAIAAS 389

Query: 492 HLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQ-------YLMDIVGPELSEPANSISLFK 551
           HL  ++  R     H  A+K Y LL +GDF Q        LM +   + +  ++ +  F+
Sbjct: 390 HLWQLVVVRADLNGHLKALKDYFLLEKGDFFQCFLEESRQLMRLPPRQSTGESDLMVPFQ 449

Query: 552 LSGLLETA-------------------IRSSNAQYDDPDILDRLKVKM-----MPHGTGD 611
           L+     A                   +RSS A       + R KV +     +   T  
Sbjct: 450 LAATKTIAEEDKYFSRVSLRMPSFGVTVRSSQAD------MVRSKVSLTGKANLTSDTSV 509

Query: 612 RGWDVFSLEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTW-KTMKPNCITS 671
            GWD  +LEY    P+   FT+ V+SKYL++F +L +L+R +  L  +W   M  + I S
Sbjct: 510 DGWDAIALEYSVDWPMQLFFTQEVLSKYLKVFQYLIRLKRTQMELEKSWASVMHQDHIES 569

Query: 672 CSLTK--LHHGVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAA 731
               K  L+     Q    +R    +   M   + NLQ+YI  +V+E  W      +  +
Sbjct: 570 AQHRKDGLNGSTSQQRRQGIRPMWRVREHMAFLIRNLQFYIQVDVIESQWKVLQTHIHDS 629

Query: 732 MDLDDLLAAHEKYLHSIFEKSLLGEQSQPLCKSLFVLFDLILRFRSNADRLYEGIHELQC 764
            D  +L+  H++YL ++  +S L   S  + + L  +  L L+F  N +           
Sbjct: 630 QDFTELVGFHQEYLSALISQSFLDIGS--VSRILDSIMKLCLQFCWNIEN---------- 689

BLAST of Carg25727 vs. TAIR 10
Match: AT1G80260.1 (Spc97 / Spc98 family of spindle pole body (SBP) component )

HSP 1 Score: 64.7 bits (156), Expect = 3.9e-10
Identity = 60/286 (20.98%), Postives = 116/286 (40.56%), Query Frame = 0

Query: 450 IDKHLLDVMHKRYKFKDHCLAIKRYLLLGQGDFVQYLMDIVGPELSEPANSISLFKLSGL 509
           I K +L  +   +K       ++   LLG GD +Q+ + ++   L +  +S   F+L+ +
Sbjct: 640 IGKVILSKLMNDWKLMHELAVLRAIYLLGSGDLLQHFLTVIFDRLGKGESSNDDFELNII 699

Query: 510 LETAIRSS--------------NAQYDDPDILDRLKV-------KMMPHGTGDRGWDVFS 569
           L+ +IR+S              +   +D D  D+  +       K   +  G    +   
Sbjct: 700 LQESIRNSADAMLLSSPDSLVVSISREDRDKDDKGDIIPLSSTRKSRVNSFGIDCLESLK 759

Query: 570 LEYEARVPLDTVFTESVMSKYLRIFNFLWKLRRVEHALIGTWKTMKPNCITSCSLTKLHH 629
             Y+   PL+ +     + KY ++  FL K++R ++ L    + M     ++  + K H 
Sbjct: 760 FTYKVPWPLELIANSEAIKKYNQVMGFLLKVKRAKYVLDKARRWMWKGKGSATKIRKHHW 819

Query: 630 GVKLQLLSTLRRCQVLWVEMNHFVSNLQYYIMFEVLEVSWSDFSNEMEAAMDLDDLLAAH 689
            ++ +LL              +FV     Y+M  V   +W +    M  A  LD+++  H
Sbjct: 820 LLEQKLL--------------NFVDAFHQYVMDRVYHTAWRELCEAMVKAGSLDEVIYVH 879

Query: 690 EKYLHSIFEKSLLGEQS--QPLCKSLFVLFDLILRFRSNADRLYEG 713
           E YL SI  +  + ++     +   + ++  L L F S    L  G
Sbjct: 880 ETYLLSIQRQCFVVQEKLWAIIASRINMILGLALEFYSIQQTLSSG 911