Carg25726 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25726
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING-type E3 ubiquitin transferase
Location	Carg_Chr03: 1197234 .. 1198963 (+)
RNA-Seq Expression	Carg25726
Synteny	Carg25726

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTAGTTGATTCTCATCCTTCAGTTTCAGGGCATGACATAGCAATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTTCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAACTGCTCGAGTGCCCTGTCTGCATGAATCTGATGTACCCTCCAATTTACCAGGTATGTACTTCATATCCTGTTTTTTCTCTTGTACTTCTCATTATAATTTAAGCTATGCTCGGGAGTGCTCGGAGTGCTTTCAGCGTCGAAAAAAGTACTTTTTCTGTATGGCTTAATTGCTTAATTCTTGATTCGAAGGTTGATTTGGTCTCTGTAGTGTGGCGAATTCTCCATATTACTGGTTCTTTAGTTCTTTTCTTAATATTAAGGTTGTATTGAAGACTTAGAACATCTCATTATTCTTGAATCTTTACGTCATGGAGGTGTGATATATTTTTCTGCTTCTGCTTTGAATCGAACTTTCTTGACTGGCTTTTGAACCTTAGTGCCCGAATGGCCACACGTTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTTCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCGGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCGCACGAAGTTGAAAACGCCACATGGATGCTAACGGTAGGTGCTTGGCTCTATATAGTATTAGTATACTTTCTCACCATGCTTCACTTCACATAACTTAACTATGTAAGCCATATATACAAGTTTTCTTGAAAATCAAATGTATAGATGGTCGTCCCGTGAGTATATATATAAATATATATTTCGATAAACCGTGTAATCTGAACGATGATAATTGCTAGCTAAAGGTCTGAAAAACCGGGTTGTCTTCCATCTTTGAGCATTGGAATATCTTATAGTAGTTTGCAATGACAGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGACGAGAGCTGAAGCTGAGAATCACGGGTCGAATATGGAAAGAAGAATGAGAGCAGGAGCAAGGGCCTAAGCTAAGCTCTTGTGGTGTTCAGGTGAATGTATCATTGTACAGGTAAAACAAGTTCTCCAAGTGCTGAAAAAGCTCTTAGGAGAAACTTCCAAGCTAATATAAAAAATGTAACCTACAAAATGAATCAAAGAAGCTTTTATGTATTTATAGTTCGAGATACGTTTCAATCTAGTGTCTCTGGTTTTTTATGTTATTA

mRNA sequence

ATGGCCATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTAGTTGATTCTCATCCTTCAGTTTCAGGGCATGACATAGCAATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTTCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAACTGCTCGAGTGCCCTGTCTGCATGAATCTGATGTACCCTCCAATTTACCAGTGCCCGAATGGCCACACGTTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTTCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCGGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCGCACGAAGTTGAAAACGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGACGAGAGCTGAAGCTGAGAATCACGGGTCGAATATGGAAAGAAGAATGAGAGCAGGAGCAAGGGCCTAAGCTAAGCTCTTGTGGTGTTCAGGTGAATGTATCATTGTACAGGTAAAACAAGTTCTCCAAGTGCTGAAAAAGCTCTTAGGAGAAACTTCCAAGCTAATATAAAAAATGTAACCTACAAAATGAATCAAAGAAGCTTTTATGTATTTATAGTTCGAGATACGTTTCAATCTAGTGTCTCTGGTTTTTTATGTTATTA

Coding sequence (CDS)

ATGGCCATGGCTCCAGGAAGCAGTACCTGCAAAGAAGTAGTTGATTCTCATCCTTCAGTTTCAGGGCATGACATAGCAATGTCTAAATCAGAGAATAATTCTACATCTTCAAAAGTTCTACTTGGTTCGAGTGGGAAGCTTGGAGTTTATTCAACCAATGGCGTGCAAGAACTGCTCGAGTGCCCTGTCTGCATGAATCTGATGTACCCTCCAATTTACCAGTGCCCGAATGGCCACACGTTGTGCTCAAACTGCAAGATCAGAGTTAGCAACTGCTGCCCGACCTGTCGTCATGAACTCGGAAACATAAGGTGCTTGGCATTGGAGAAGGTAGCTGAGTCGCTGGAAATCCCCTGCAGATACCAGAATTTGGGTTGCCAAGATATTTTTCCTTACTACAGTAAGCTTAAGCATGAGCAACACTGTCGATTCCGTCCTTACAACTGCCCTTATGCTGGATCGGAGTGTTCGGTCACGGGTGACATCCCGTCTCTTGTTGCACATCTCAAAGATGATCACAAGGTTGACATGCACGATGGGTGTACCTTCAATCATCGCTATGTGAAATCAAATCCGCACGAAGTTGAAAACGCCACATGGATGCTAACGGTTTTCAACTGCTTTGGAAGACAGTTCTGCTTGCATTTCGAGGCGTTCCAGCTAGGAACAGCGCCTGTTTACATGGCGTTCTTACGATTCATGGGCGAAGATAACGAAGCAAAGAAGTTCAGCTACAGTTTGGAAGTGGGTCACAACGGGCGTAAACTGATATGGCAAGGAATTCCGAGAGGCATCCGTGACAGTCACCGAAAAGTTCGTGACAGTCAAGACGGTCTCATCATTCAAAGAAACCTGGCACTGTATTTTTCGGGAGGAGAAAGACGAGAGCTGAAGCTGAGAATCACGGGTCGAATATGGAAAGAAGAATGA

Protein sequence

MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE

Homology

[Close]

BLAST of Carg25726 vs. NCBI nr
Match: KAG6603259.1 (E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033559.1 E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 659.1 bits (1699), Expect = 1.9e-185
Identity = 309/309 (100.00%), Postives = 309/309 (100.00%), Query Frame = 0


Query: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60
           MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE
Sbjct: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60

Query: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120
           CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR
Sbjct: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG
Sbjct: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240

Query: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300
           KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR
Sbjct: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300

Query: 301 ITGRIWKEE 310
           ITGRIWKEE
Sbjct: 301 ITGRIWKEE 309

[Close]

BLAST of Carg25726 vs. NCBI nr
Match: XP_022967856.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543585.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543586.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023543587.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 656.8 bits (1693), Expect = 9.5e-185
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60
           MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE
Sbjct: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60

Query: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120
           CPVC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR
Sbjct: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG
Sbjct: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240

Query: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300
           KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR
Sbjct: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300

Query: 301 ITGRIWKEE 310
           ITGRIWKEE
Sbjct: 301 ITGRIWKEE 309

[Close]

BLAST of Carg25726 vs. NCBI nr
Match: XP_022928730.1 (E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928731.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928732.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928733.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_022928734.1 E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 655.2 bits (1689), Expect = 2.8e-184
Identity = 307/309 (99.35%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60
           MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE
Sbjct: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60

Query: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120
           CPVC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR
Sbjct: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG
Sbjct: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240

Query: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300
           KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLR
Sbjct: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLR 300

Query: 301 ITGRIWKEE 310
           ITGRIWKEE
Sbjct: 301 ITGRIWKEE 309

[Close]

BLAST of Carg25726 vs. NCBI nr
Match: XP_038881778.1 (E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida])

HSP 1 Score: 632.9 bits (1631), Expect = 1.5e-177
Identity = 294/307 (95.77%), Postives = 301/307 (98.05%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPGSSTCKEVVDSHPSVSGHDIAMSK+ENNSTSSK  LG SGK+GVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKEVVDSHPSVSGHDIAMSKTENNSTSSKAALGLSGKIGVYSTNGVQELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPIYQCP+GHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ
Sbjct: 61  VCTNLMYPPIYQCPSGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCR+RPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRYRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFG+QFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGKQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 307

[Close]

BLAST of Carg25726 vs. NCBI nr
Match: XP_023518392.1 (E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >XP_023518393.1 E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >KAG6595627.1 E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027600.1 E3 ubiquitin-protein ligase SINAT2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.5 bits (1591), Expect = 6.4e-173
Identity = 291/307 (94.79%), Postives = 296/307 (96.42%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPGSSTCK+ VDSHPSVSGHDIA+SKSENN T+SK  LG  GKLGVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHPSVSGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPCRYQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 306

[Close]

BLAST of Carg25726 vs. ExPASy Swiss-Prot
Match: Q9M2P4 (E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=1 SV=1)

HSP 1 Score: 545.8 bits (1405), Expect = 3.1e-154
Identity = 252/308 (81.82%), Postives = 275/308 (89.29%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE-NNSTSSKVLLGSSGKLGVYSTNGVQELLEC 62
           MAPG S  KEV++S+ +   +++  +K E NN+  +K      GK G++S NGV ELLEC
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLEC 60

Query: 63  PVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRY 122
           PVC NLMYPPI+QCPNGHTLCSNCK+RV N CPTCR+ELGNIRCLALEKVAESLE+PCRY
Sbjct: 61  PVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRY 120

Query: 123 QNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGC 182
           QNLGC DIFPYYSKLKHEQHCRFRPY CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGC
Sbjct: 121 QNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGC 180

Query: 183 TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAK 242
           TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAK
Sbjct: 181 TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAK 240

Query: 243 KFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRI 302
           KFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+R+ELKLR+
Sbjct: 241 KFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRV 300

Query: 303 TGRIWKEE 310
           TGRIWKEE
Sbjct: 301 TGRIWKEE 308

[Close]

BLAST of Carg25726 vs. ExPASy Swiss-Prot
Match: P93748 (Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=SINAT1 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 4.2e-151
Identity = 249/307 (81.11%), Postives = 273/307 (88.93%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPG S  KE ++S+ +   +++ M+K E NS  +K   GS GK   +S+NGV ELLECP
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPI+QCPNGHTLCS+CK+RV N CPTCR+ELGNIRCLALEKVAESLE+PCRYQ
Sbjct: 61  VCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCRFR Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG ++ ELKLR+T
Sbjct: 241 FSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 305

[Close]

BLAST of Carg25726 vs. ExPASy Swiss-Prot
Match: Q10L91 (E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS1 PE=1 SV=1)

HSP 1 Score: 470.3 bits (1209), Expect = 1.7e-131
Identity = 210/266 (78.95%), Postives = 236/266 (88.72%), Query Frame = 0


Query: 44  SGKLGVYSTNGVQELLECPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNI 103
           S K  V  ++ V+ELLECPVC++ MYPPI+QC NGHTLCS CK RV N CPTCRHELGNI
Sbjct: 36  SPKPNVVVSSSVRELLECPVCLSAMYPPIHQCSNGHTLCSGCKPRVHNRCPTCRHELGNI 95

Query: 104 RCLALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIP 163
           RCLALEKVA SLE+PC+YQN GC  I+PYY KLKHE  C++RPY+CPYAGSEC+V GDIP
Sbjct: 96  RCLALEKVAASLELPCKYQNFGCVGIYPYYCKLKHESQCQYRPYSCPYAGSECTVAGDIP 155

Query: 164 SLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGT 223
            LV HLKDDHKVDMH+GCTFNHRYVKSNPHEVENATWMLTVF+CFG+ FCLHFEAFQLG 
Sbjct: 156 YLVNHLKDDHKVDMHNGCTFNHRYVKSNPHEVENATWMLTVFSCFGQYFCLHFEAFQLGM 215

Query: 224 APVYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQR 283
           APVY+AFLRFMG+D EAK +SYSLEVG  GRK+IWQG+PR IRDSHRKVRDS DGLIIQR
Sbjct: 216 APVYIAFLRFMGDDLEAKNYSYSLEVGGTGRKMIWQGVPRSIRDSHRKVRDSYDGLIIQR 275

Query: 284 NLALYFSGGERRELKLRITGRIWKEE 310
           N+AL+FSGGER+ELKLR+TGRIWKE+
Sbjct: 276 NMALFFSGGERKELKLRVTGRIWKEQ 301

[Close]

BLAST of Carg25726 vs. ExPASy Swiss-Prot
Match: Q84JL3 (E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=1 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 1.2e-129
Identity = 216/294 (73.47%), Postives = 246/294 (83.67%), Query Frame = 0


Query: 16  SHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCMNLMYPPIYQC 75
           S+ SVS H         N+ ++ V   +SG L   +T  V ELLECPVC N MYPPI+QC
Sbjct: 26  SYASVSKH------HHTNNNTTNVNAAASGLLP--TTTSVHELLECPVCTNSMYPPIHQC 85

Query: 76  PNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSK 135
            NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAESLE+PC++ +LGC +IFPYYSK
Sbjct: 86  HNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSK 145

Query: 136 LKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEV 195
           LKHE  C FRPY+CPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EV
Sbjct: 146 LKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREV 205

Query: 196 ENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRK 255
           ENATWMLTVF+CFG+ FCLHFEAFQLG APVYMAFLRFMG++ EA+ ++YSLEVG  GRK
Sbjct: 206 ENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRK 265

Query: 256 LIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 310
           LIW+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 266 LIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

[Close]

BLAST of Carg25726 vs. ExPASy Swiss-Prot
Match: Q9STN8 (E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=1 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 1.6e-126
Identity = 206/303 (67.99%), Postives = 242/303 (79.87%), Query Frame = 0


Query: 7   SSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCMN 66
           SST  E+  +      +  + +K+   + ++ V+    G         V ELLECPVC  
Sbjct: 10  SSTGNEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTY 69

Query: 67  LMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGC 126
            MYPPI+QC NGHTLCS CK+RV N CPTCR ELG+IRCLALEKVAESLE+PC++ NLGC
Sbjct: 70  SMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGC 129

Query: 127 QDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHR 186
            +IFPYYSKLKHE  C FRPY+CPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHR
Sbjct: 130 PEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHR 189

Query: 187 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYS 246
           YVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLG  PVYMAFLRFMG++ +A+ +SYS
Sbjct: 190 YVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYS 249

Query: 247 LEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIW 306
           LEVG +GRKL W+G PR IRDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IW
Sbjct: 250 LEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIW 309

Query: 307 KEE 310
           KE+
Sbjct: 310 KEQ 312

[Close]

BLAST of Carg25726 vs. ExPASy TrEMBL
Match: A0A6J1HT85 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 PE=3 SV=1)

HSP 1 Score: 656.8 bits (1693), Expect = 4.6e-185
Identity = 308/309 (99.68%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60
           MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE
Sbjct: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60

Query: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120
           CPVC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR
Sbjct: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG
Sbjct: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240

Query: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300
           KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR
Sbjct: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300

Query: 301 ITGRIWKEE 310
           ITGRIWKEE
Sbjct: 301 ITGRIWKEE 309

[Close]

BLAST of Carg25726 vs. ExPASy TrEMBL
Match: A0A6J1ESE8 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553 PE=3 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 1.3e-184
Identity = 307/309 (99.35%), Postives = 308/309 (99.68%), Query Frame = 0


Query: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60
           MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE
Sbjct: 1   MAMAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLE 60

Query: 61  CPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120
           CPVC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR
Sbjct: 61  CPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCR 120

Query: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180
           YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG
Sbjct: 121 YQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDG 180

Query: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240
           CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA
Sbjct: 181 CTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEA 240

Query: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLR 300
           KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLR
Sbjct: 241 KKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLR 300

Query: 301 ITGRIWKEE 310
           ITGRIWKEE
Sbjct: 301 ITGRIWKEE 309

[Close]

BLAST of Carg25726 vs. ExPASy TrEMBL
Match: A0A6J1EBG4 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512 PE=3 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 4.1e-173
Identity = 290/307 (94.46%), Postives = 296/307 (96.42%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPGSSTCK+ VDSHPS+SGHDIA+SKSENN T+SK  LG  GKLGVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHPSISGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPCRYQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 306

[Close]

BLAST of Carg25726 vs. ExPASy TrEMBL
Match: A0A6J1HS09 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 PE=3 SV=1)

HSP 1 Score: 613.6 bits (1581), Expect = 4.5e-172
Identity = 290/307 (94.46%), Postives = 295/307 (96.09%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPGSSTCK+ VDSH SVSGHDIA+SKSENN T+SK  LG  GKLGVYSTNGVQELLECP
Sbjct: 1   MAPGSSTCKK-VDSHLSVSGHDIAISKSENNPTTSKASLGLGGKLGVYSTNGVQELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPIYQCPNGHTLCSNCKIRVSN CPTCRHELGNIRCLALEKVAESLEIPCRYQ
Sbjct: 61  VCTNLMYPPIYQCPNGHTLCSNCKIRVSNSCPTCRHELGNIRCLALEKVAESLEIPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDI SLVAHLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIQSLVAHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG+DNEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDDNEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG NGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER+ELKLRIT
Sbjct: 241 FSYSLEVGGNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERQELKLRIT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 306

[Close]

BLAST of Carg25726 vs. ExPASy TrEMBL
Match: A0A6J1DL03 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111022066 PE=3 SV=1)

HSP 1 Score: 612.5 bits (1578), Expect = 1.0e-171
Identity = 288/315 (91.43%), Postives = 296/315 (93.97%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE--------NNSTSSKVLLGSSGKLGVYSTNG 62
           MAPG+S CKEVVDSHPSVSGHDIA+SKSE        +NS S+KVLLG  GKLGVYSTNG
Sbjct: 1   MAPGTSACKEVVDSHPSVSGHDIAVSKSESNSNSNSNSNSASTKVLLGLGGKLGVYSTNG 60

Query: 63  VQELLECPVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 122
           VQELLECPVC NLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES
Sbjct: 61  VQELLECPVCTNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAES 120

Query: 123 LEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHK 182
           LEIPCRY +LGC DIFPYYSKLKHEQHCRFRPYNCPYAGSECSV+GDIPSLV HLKDDHK
Sbjct: 121 LEIPCRYHSLGCLDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVSGDIPSLVTHLKDDHK 180

Query: 183 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFM 242
           VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFM
Sbjct: 181 VDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFM 240

Query: 243 GEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 302
           G+DNEAKKFSYSLEVG NGRKLIW GIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER
Sbjct: 241 GDDNEAKKFSYSLEVGGNGRKLIWHGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGER 300

Query: 303 RELKLRITGRIWKEE 310
           +ELKLRITGRIWKEE
Sbjct: 301 QELKLRITGRIWKEE 315

[Close]

BLAST of Carg25726 vs. TAIR 10
Match: AT3G58040.1 (seven in absentia of Arabidopsis 2 )

HSP 1 Score: 545.8 bits (1405), Expect = 2.2e-155
Identity = 252/308 (81.82%), Postives = 275/308 (89.29%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSE-NNSTSSKVLLGSSGKLGVYSTNGVQELLEC 62
           MAPG S  KEV++S+ +   +++  +K E NN+  +K      GK G++S NGV ELLEC
Sbjct: 1   MAPGGSALKEVMESNSTGMDYEVKTAKVEVNNNKPTKPGSAGIGKYGIHSNNGVYELLEC 60

Query: 63  PVCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRY 122
           PVC NLMYPPI+QCPNGHTLCSNCK+RV N CPTCR+ELGNIRCLALEKVAESLE+PCRY
Sbjct: 61  PVCTNLMYPPIHQCPNGHTLCSNCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRY 120

Query: 123 QNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGC 182
           QNLGC DIFPYYSKLKHEQHCRFRPY CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGC
Sbjct: 121 QNLGCHDIFPYYSKLKHEQHCRFRPYTCPYAGSECSVTGDIPTLVVHLKDDHKVDMHDGC 180

Query: 183 TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAK 242
           TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAK
Sbjct: 181 TFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAK 240

Query: 243 KFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRI 302
           KFSYSLEVG +GRKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSGG+R+ELKLR+
Sbjct: 241 KFSYSLEVGAHGRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGGDRQELKLRV 300

Query: 303 TGRIWKEE 310
           TGRIWKEE
Sbjct: 301 TGRIWKEE 308

[Close]

BLAST of Carg25726 vs. TAIR 10
Match: AT2G41980.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 535.4 bits (1378), Expect = 3.0e-152
Identity = 249/307 (81.11%), Postives = 273/307 (88.93%), Query Frame = 0


Query: 3   MAPGSSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECP 62
           MAPG S  KE ++S+ +   +++ M+K E NS  +K   GS GK   +S+NGV ELLECP
Sbjct: 1   MAPGGSALKEALESNSTGVDYEVKMAKVEANSKPTKSGSGSIGKF--HSSNGVYELLECP 60

Query: 63  VCMNLMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQ 122
           VC NLMYPPI+QCPNGHTLCS+CK+RV N CPTCR+ELGNIRCLALEKVAESLE+PCRYQ
Sbjct: 61  VCTNLMYPPIHQCPNGHTLCSSCKLRVQNTCPTCRYELGNIRCLALEKVAESLEVPCRYQ 120

Query: 123 NLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCT 182
           NLGCQDIFPYYSKLKHEQHCRFR Y+CPYAGSECSVTGDIP+LV HLKDDHKVDMHDGCT
Sbjct: 121 NLGCQDIFPYYSKLKHEQHCRFRSYSCPYAGSECSVTGDIPTLVDHLKDDHKVDMHDGCT 180

Query: 183 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKK 242
           FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLG APVYMAFLRFMG++NEAKK
Sbjct: 181 FNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGMAPVYMAFLRFMGDENEAKK 240

Query: 243 FSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRIT 302
           FSYSLEVG + RKL WQGIPR IRDSHRKVRDSQDGLII RNLALYFSG ++ ELKLR+T
Sbjct: 241 FSYSLEVGAHSRKLTWQGIPRSIRDSHRKVRDSQDGLIIPRNLALYFSGSDKEELKLRVT 300

Query: 303 GRIWKEE 310
           GRIWKEE
Sbjct: 301 GRIWKEE 305

[Close]

BLAST of Carg25726 vs. TAIR 10
Match: AT3G61790.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 464.2 bits (1193), Expect = 8.5e-131
Identity = 216/294 (73.47%), Postives = 246/294 (83.67%), Query Frame = 0


Query: 16  SHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCMNLMYPPIYQC 75
           S+ SVS H         N+ ++ V   +SG L   +T  V ELLECPVC N MYPPI+QC
Sbjct: 26  SYASVSKH------HHTNNNTTNVNAAASGLLP--TTTSVHELLECPVCTNSMYPPIHQC 85

Query: 76  PNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGCQDIFPYYSK 135
            NGHTLCS CK RV N CPTCR ELG+IRCLALEKVAESLE+PC++ +LGC +IFPYYSK
Sbjct: 86  HNGHTLCSTCKARVHNRCPTCRQELGDIRCLALEKVAESLELPCKHMSLGCPEIFPYYSK 145

Query: 136 LKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHRYVKSNPHEV 195
           LKHE  C FRPY+CPYAGSECSVTGDIP LVAHL+DDHKVDMH GCTFNHRYVKSNP EV
Sbjct: 146 LKHETVCNFRPYSCPYAGSECSVTGDIPFLVAHLRDDHKVDMHSGCTFNHRYVKSNPREV 205

Query: 196 ENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYSLEVGHNGRK 255
           ENATWMLTVF+CFG+ FCLHFEAFQLG APVYMAFLRFMG++ EA+ ++YSLEVG  GRK
Sbjct: 206 ENATWMLTVFHCFGQYFCLHFEAFQLGMAPVYMAFLRFMGDETEARNYNYSLEVGGYGRK 265

Query: 256 LIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIWKEE 310
           LIW+G PR +RDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TGRIWKE+
Sbjct: 266 LIWEGTPRSVRDSHRKVRDSHDGLIIQRNMALFFSGGDRKELKLRVTGRIWKEQ 311

[Close]

BLAST of Carg25726 vs. TAIR 10
Match: AT4G27880.1 (Protein with RING/U-box and TRAF-like domains )

HSP 1 Score: 453.8 bits (1166), Expect = 1.1e-127
Identity = 206/303 (67.99%), Postives = 242/303 (79.87%), Query Frame = 0


Query: 7   SSTCKEVVDSHPSVSGHDIAMSKSENNSTSSKVLLGSSGKLGVYSTNGVQELLECPVCMN 66
           SST  E+  +      +  + +K+   + ++ V+    G         V ELLECPVC  
Sbjct: 10  SSTGNEIHQNGNGHQSYQFSSTKTHGGAAAAAVVTNIVGPTATAPATSVYELLECPVCTY 69

Query: 67  LMYPPIYQCPNGHTLCSNCKIRVSNCCPTCRHELGNIRCLALEKVAESLEIPCRYQNLGC 126
            MYPPI+QC NGHTLCS CK+RV N CPTCR ELG+IRCLALEKVAESLE+PC++ NLGC
Sbjct: 70  SMYPPIHQCHNGHTLCSTCKVRVHNRCPTCRQELGDIRCLALEKVAESLELPCKFYNLGC 129

Query: 127 QDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSLVAHLKDDHKVDMHDGCTFNHR 186
            +IFPYYSKLKHE  C FRPY+CPYAGSEC + GDIP LVAHL+DDHKVDMH G TFNHR
Sbjct: 130 PEIFPYYSKLKHESLCNFRPYSCPYAGSECGIVGDIPFLVAHLRDDHKVDMHAGSTFNHR 189

Query: 187 YVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAPVYMAFLRFMGEDNEAKKFSYS 246
           YVKSNP EVENATWMLTVF+CFG+ FCLHFEAFQLG  PVYMAFLRFMG++ +A+ +SYS
Sbjct: 190 YVKSNPREVENATWMLTVFHCFGQYFCLHFEAFQLGMGPVYMAFLRFMGDEEDARSYSYS 249

Query: 247 LEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNLALYFSGGERRELKLRITGRIW 306
           LEVG +GRKL W+G PR IRDSHRKVRDS DGLIIQRN+AL+FSGG+R+ELKLR+TG+IW
Sbjct: 250 LEVGGSGRKLTWEGTPRSIRDSHRKVRDSNDGLIIQRNMALFFSGGDRKELKLRVTGKIW 309

Query: 307 KEE 310
           KE+
Sbjct: 310 KEQ 312

[Close]

BLAST of Carg25726 vs. TAIR 10
Match: AT5G53360.1 (TRAF-like superfamily protein )

HSP 1 Score: 360.9 bits (925), Expect = 1.0e-99
Identity = 158/204 (77.45%), Postives = 182/204 (89.22%), Query Frame = 0


Query: 106 LALEKVAESLEIPCRYQNLGCQDIFPYYSKLKHEQHCRFRPYNCPYAGSECSVTGDIPSL 165
           +ALEKVAESLE+PC+Y NLGC  IFPYYSKLKHE  C FRPY+CPYAGSEC+  GDI  L
Sbjct: 15  IALEKVAESLELPCKYYNLGCLGIFPYYSKLKHESQCNFRPYSCPYAGSECAAVGDITFL 74

Query: 166 VAHLKDDHKVDMHDGCTFNHRYVKSNPHEVENATWMLTVFNCFGRQFCLHFEAFQLGTAP 225
           VAHL+DDHKVDMH GCTFNHRYVKSNP EVENATWMLTVF CFG+ FCLHFEAFQLG AP
Sbjct: 75  VAHLRDDHKVDMHTGCTFNHRYVKSNPREVENATWMLTVFQCFGQYFCLHFEAFQLGMAP 134

Query: 226 VYMAFLRFMGEDNEAKKFSYSLEVGHNGRKLIWQGIPRGIRDSHRKVRDSQDGLIIQRNL 285
           VYMAFLRFMG++++A+ ++YSLEVG +GRK  W+G PR +RDSHRKVRDS DGLIIQRN+
Sbjct: 135 VYMAFLRFMGDEDDARNYTYSLEVGGSGRKQTWEGTPRSVRDSHRKVRDSHDGLIIQRNM 194

Query: 286 ALYFSGGERRELKLRITGRIWKEE 310
           AL+FSGG+++ELKLR+TGRIWKE+
Sbjct: 195 ALFFSGGDKKELKLRVTGRIWKEQ 218

The following BLAST results are available for this feature:

BLAST of Carg25726 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6603259.1	1.9e-185	100.00	E3 ubiquitin-protein ligase SINAT2, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022967856.1	9.5e-185	99.68	E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita maxima] >XP_023543...	[more]
XP_022928730.1	2.8e-184	99.35	E3 ubiquitin-protein ligase SINAT2-like isoform X1 [Cucurbita moschata] >XP_0229...	[more]
XP_038881778.1	1.5e-177	95.77	E3 ubiquitin-protein ligase SINAT2-like [Benincasa hispida] >XP_038881779.1 E3 u...	[more]
XP_023518392.1	6.4e-173	94.79	E3 ubiquitin-protein ligase SINAT2-like [Cucurbita pepo subsp. pepo] >XP_0235183...	[more]

BLAST of Carg25726 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2P4	3.1e-154	81.82	E3 ubiquitin-protein ligase SINAT2 OS=Arabidopsis thaliana OX=3702 GN=SINAT2 PE=...	[more]
P93748	4.2e-151	81.11	Putative E3 ubiquitin-protein ligase SINAT1 OS=Arabidopsis thaliana OX=3702 GN=S...	[more]
Q10L91	1.7e-131	78.95	E3 ubiquitin-protein ligase DIS1 OS=Oryza sativa subsp. japonica OX=39947 GN=DIS...	[more]
Q84JL3	1.2e-129	73.47	E3 ubiquitin-protein ligase SINAT3 OS=Arabidopsis thaliana OX=3702 GN=SINAT3 PE=...	[more]
Q9STN8	1.6e-126	67.99	E3 ubiquitin-protein ligase SINAT4 OS=Arabidopsis thaliana OX=3702 GN=SINAT4 PE=...	[more]

BLAST of Carg25726 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HT85	4.6e-185	99.68	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111467239 P...	[more]
A0A6J1ESE8	1.3e-184	99.35	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111435553...	[more]
A0A6J1EBG4	4.1e-173	94.46	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111432512...	[more]
A0A6J1HS09	4.5e-172	94.46	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111466063 P...	[more]
A0A6J1DL03	1.0e-171	91.43	RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC11102206...	[more]

BLAST of Carg25726 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G58040.1	2.2e-155	81.82	seven in absentia of Arabidopsis 2	[more]
AT2G41980.1	3.0e-152	81.11	Protein with RING/U-box and TRAF-like domains	[more]
AT3G61790.1	8.5e-131	73.47	Protein with RING/U-box and TRAF-like domains	[more]
AT4G27880.1	1.1e-127	67.99	Protein with RING/U-box and TRAF-like domains	[more]
AT5G53360.1	1.0e-99	77.45	TRAF-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 114..168 e-value: 1.9E-18 score: 68.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 22..113 e-value: 4.9E-9 score: 37.8
IPR018121	Seven-in-absentia protein, TRAF-like domain	PFAM	PF03145	Sina	coord: 103..302 e-value: 2.8E-80 score: 269.0
IPR008974	TRAF-like	GENE3D	2.60.210.10	Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A	coord: 169..302 e-value: 6.3E-37 score: 128.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 23..37
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..37
None	No IPR available	PANTHER	PTHR10315	E3 UBIQUITIN PROTEIN LIGASE SIAH	coord: 3..309
None	No IPR available	PANTHER	PTHR10315:SF95	E3 UBIQUITIN-PROTEIN LIGASE SINAT1-RELATED	coord: 3..309
None	No IPR available	CDD	cd16571	RING-HC_SIAHs	coord: 59..97 e-value: 1.76154E-14 score: 64.2282
None	No IPR available	SUPERFAMILY	49599	TRAF domain-like	coord: 114..306
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 60..102
IPR013010	Zinc finger, SIAH-type	PROSITE	PS51081	ZF_SIAH	coord: 114..174 score: 14.50975
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 61..97 score: 9.568184

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25726-RA	Carg25726-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007275	multicellular organism development
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0016874	ligase activity
molecular_function	GO:0005515	protein binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0008270	zinc ion binding