Carg25704 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25704
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pyridoxal 5'-phosphate synthase
Location	Carg_Chr14: 14726474 .. 14728078 (+)
RNA-Seq Expression	Carg25704
Synteny	Carg25704

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGTACATTTCCCTCTCTCTATTTCTCTTCAACTTGAACTACCCTTTCCCTTTTTCACAGTCGGGTTTTATTTTCTTAAATGGAATTTGTCTTCAGGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGGTTCTTCTACGATGAAGCTCTATATTTCGCTTTAGATCGCTCAATTCATACGCCCATTTCTTGTTGTTCTATGGTTTCCGACGCAATCGTTATCTGGGTTTCTGGATTTTCAATTATGTAATTCATATGTTTGGCTACAATAAGATAAGCTATAAAATATGCTCAGTACTGGGAATTTGTCTGCGATGGATTAGGTCAGGTTTGTGATATTAAGCATCTGAGTTTTGTCCTGCAAATGAACTTTTCGCTTTTGGTTGATTCTGCAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGGTAACTATTCTCGTCTCTTCCTGCCTCTTAATACAGTTCATAAAACAAATGATTTGTTCTTGCTGGTCTATGTAGCTTATCTTATTTACATAACTGCAAATATGTGCCTATTTGAGTCAAATTTTGGACTTGGAATGTATTTGTTGTTCAAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGGTTGTGGGCATGACCCTTTCCTCTTATAACTTACAACTTGTTCACTTTCTTGTTCACTTCATTCAACTCAGTATAATCTTATATATTTATTAATGCAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTTTGTAGTATATATATATGTATATAGACTTTACGTTATGTTACATTTGACCGCGTTTCGCTCTCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATGTTGATAACAACAAGCTGCTACGTTGAAATGAAGTGATATAGTTAGTGAGTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTCTGATTGAACAATCATGGTTCCCTATATCTGTAGATAGGGTGTATGTAACTCAATTCTTATGCTGTTGAAGCTGAAACAAAAGGAATGAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCTCT

mRNA sequence

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAGCAATTGCACCTCGATTTGCAGGTATGTTGATAACAACAAGCTGCTACGTTGAAATGAAGTGATATAGTTAGTGAGTCATGTGTTGAAAATAATCCTCCGACCCTTTGGTTGTTAGTAGAGATGATACTCTGATTGAACAATCATGGTTCCCTATATCTGTAGATAGGGTGTATGTAACTCAATTCTTATGCTGTTGAAGCTGAAACAAAAGGAATGAAAGTTGGAATGAGTTGTTTGAAGCATGAAATATGCAAAGAAACAGAAGGATAGGAAAGGCTCT

Coding sequence (CDS)

ATGGGGCGAATACATAGCACAGCAGTAGGCGAACTAGCGGGCGATTTGGAAATGGGATCGGCAGCGGCGCCATGGAAGTCCCTTCTTCTCAGTGCTTTGGAATCTAATGCCCAAATCAAGCATTCCACATTCTTGCACCTCGCAACGATTGGAGCCAATGGAAGGCCATCAAATCGCACCGTTGTTTTCAGAGGATTTGAAGAGGGCACCGATAGGATCCATATTTACACGGATTCAAGAAGTCGCAAGATTGAAGAGCTTAAAACTTGTCCATTTGCTGAGATAAGTTGGTATTTCACGGAATCATGGGAGCAATTTCGTATCAGTGGAAGAATTGAGGTTGTTGATGCGTCATGTCTTGATGCTACAAAATTTAAGAAAAGAGCAGAAGCCTGGTCCTCCATTTCTCCAAAGTCAAGAATGCAGTATTTGGGGCCTAGTCCAAATCTTCCCTATATTGCCGAACAGCCAGCCAAGGAACCTGTTCTAGATTCTTGTTCAGGTCCCGTTGATGCATTTTGTTTGCTGGTCTTGGATCCTGATCAGGTCGATTACTTGAATCTGAAGAGTGCAGAAAGGAAGTTGTTTAAAGCTATTCCATGTGTTGGAAGGGAGACATTATGGGAATCAGAGCGGATAAACCCTTAG

Protein sequence

MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFKAIPCVGRETLWESERINP

Homology

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: KAG7019079.1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 441.0 bits (1133), Expect = 5.7e-120
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL
Sbjct: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
           DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 215

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_022924710.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata] >KAG6582681.1 Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 441.0 bits (1133), Expect = 5.7e-120
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
           DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 221

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_022980848.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 395.2 bits (1014), Expect = 3.6e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180

Query: 198 AIPCVGRETLWESERINP 216
           AIPC+GRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_023527386.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023527387.1 pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 391.0 bits (1003), Expect = 6.8e-105
Identity = 190/198 (95.96%), Postives = 191/198 (96.46%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSAAAPWKSLLLSALESNAQIKHS FLHLATIG NGRPSNRTVVFRGFEEGTD IHIYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQIKHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSIS 
Sbjct: 61  DSRSRKIEELKACPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSPNLPYIAEQPAKEPVLD CSGPVDAFCLLVLDPDQVDYLNLKS ERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDPCSGPVDAFCLLVLDPDQVDYLNLKSEERKLFK 180

Query: 198 AIPCVGRETLWESERINP 216
           AIPC+GRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

[Close]

BLAST of Carg25704 vs. NCBI nr
Match: XP_022924711.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Cucurbita moschata])

HSP 1 Score: 357.8 bits (917), Expect = 6.4e-95
Identity = 183/215 (85.12%), Postives = 183/215 (85.12%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE                          
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE-------------------------- 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
                 KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 ------KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 189

[Close]

BLAST of Carg25704 vs. ExPASy Swiss-Prot
Match: Q9ZPY1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 239.2 bits (609), Expect = 4.3e-62
Identity = 111/198 (56.06%), Postives = 150/198 (75.76%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+  APWK LL  A+E+N+ + HS+++ LATIG NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 198 AIPCVGRETLWESERINP 216
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

[Close]

BLAST of Carg25704 vs. ExPASy Swiss-Prot
Match: Q7P203 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strain ATCC 12472 / DSM 30191 / JCM 1249 / NBRC 12614 / NCIMB 9131 / NCTC 9757) OX=243365 GN=pdxH PE=3 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.5e-09
Identity = 36/102 (35.29%), Postives = 59/102 (57.84%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           AQ+   T ++LA IGA+GRPS+R V+ +G E+G  ++  YT+ +SRK + L+  P+  ++
Sbjct: 40  AQVPEPTAMNLAAIGADGRPSSRIVLLKGVEDG--QLLFYTNYQSRKGQALEANPYVALN 99

Query: 97  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 135
           +++ E   Q RI G+   V     DA      +  R  AW+S
Sbjct: 100 FFWPELERQVRIEGKAARVAPEVSDAYFASRPYTSRLGAWAS 139

[Close]

BLAST of Carg25704 vs. ExPASy Swiss-Prot
Match: Q65QT9 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Mannheimia succiniciproducens (strain MBEL55E) OX=221988 GN=pdxH PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 3.1e-07
Identity = 30/102 (29.41%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           A++   T +++AT+G NG+PS+R V+ +  E     +  +T+  S K  +L   PFA ++
Sbjct: 39  AKVNEPTAMNVATVGENGKPSSRVVLLK--EVNERGLVFFTNYHSHKGRDLAVNPFAAVN 98

Query: 97  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 135
            ++ E   Q R+ GR+E +     D    +  +  R  AW+S
Sbjct: 99  LFWAELQRQVRVEGRVERISPQASDEYFASRPYTSRIGAWAS 138

[Close]

BLAST of Carg25704 vs. ExPASy Swiss-Prot
Match: A1TQY3 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC00-1) OX=397945 GN=pdxH PE=3 SV=1)

HSP 1 Score: 56.6 bits (135), Expect = 4.0e-07
Identity = 37/102 (36.27%), Postives = 54/102 (52.94%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           AQ+     + LAT+GA+ RPS R V+ +G++E    I  YT+  SRK  +L   PFA + 
Sbjct: 52  AQVPEPNAMTLATVGADLRPSTRVVLIKGYDE--RGIVWYTNYGSRKGRQLAGNPFAALQ 111

Query: 97  WYFTESWEQFRISGRIEVVDASCLDA----TKFKKRAEAWSS 135
           +++ E     RI GR+E V  +  DA         R  AW+S
Sbjct: 112 FHWVELERVVRIEGRVEKVSDAESDAYFASRPLDSRIGAWAS 151

[Close]

BLAST of Carg25704 vs. ExPASy Swiss-Prot
Match: A9AGI1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia multivorans (strain ATCC 17616 / 249) OX=395019 GN=pdxH PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 6.8e-07
Identity = 35/102 (34.31%), Postives = 53/102 (51.96%), Query Frame = 0


Query: 37  AQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEIS 96
           A++     + LAT+GA+GRPS R V+ +G +E       +T+  SRK  +L   P+A + 
Sbjct: 39  AKLPEPNTMTLATVGADGRPSARIVLVKGVDE--RGFVFFTNYESRKGRDLAAHPYAALL 98

Query: 97  WYFTESWEQFRISGRIEVVDASCLD----ATKFKKRAEAWSS 135
           +Y+ E   Q RI GR+E   A   D    +     R  AW+S
Sbjct: 99  FYWIELERQVRIEGRVEKTSAEESDRYFASRPVGSRIGAWAS 138

[Close]

BLAST of Carg25704 vs. ExPASy TrEMBL
Match: A0A6J1EFU0 (Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 2.8e-120
Identity = 215/215 (100.00%), Postives = 215/215 (100.00%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
           DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 221

[Close]

BLAST of Carg25704 vs. ExPASy TrEMBL
Match: A0A6J1J0G7 (Pyridoxal 5'-phosphate synthase OS=Cucurbita maxima OX=3661 GN=LOC111480118 PE=4 SV=1)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-106
Identity = 192/198 (96.97%), Postives = 193/198 (97.47%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSAAAPWKSLLLSALESNAQI HS FLHLATIG NGRPSNRTVVFRGFEEGTD I IYT
Sbjct: 1   MGSAAAPWKSLLLSALESNAQITHSRFLHLATIGGNGRPSNRTVVFRGFEEGTDGIRIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP
Sbjct: 61  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK
Sbjct: 121 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 180

Query: 198 AIPCVGRETLWESERINP 216
           AIPC+GRETLWESERINP
Sbjct: 181 AIPCLGRETLWESERINP 198

[Close]

BLAST of Carg25704 vs. ExPASy TrEMBL
Match: A0A6J1EA79 (Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE=4 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 3.1e-95
Identity = 183/215 (85.12%), Postives = 183/215 (85.12%), Query Frame = 0


Query: 1   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 60
           MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT
Sbjct: 7   MGRIHSTAVGELAGDLEMGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRT 66

Query: 61  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCL 120
           VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE                          
Sbjct: 67  VVFRGFEEGTDRIHIYTDSRSRKIEELKTCPFAE-------------------------- 126

Query: 121 DATKFKKRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 180
                 KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP
Sbjct: 127 ------KRAEAWSSISPKSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDP 186

Query: 181 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 216
           DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP
Sbjct: 187 DQVDYLNLKSAERKLFKAIPCVGRETLWESERINP 189

[Close]

BLAST of Carg25704 vs. ExPASy TrEMBL
Match: A0A5A7U3K2 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G00920 PE=4 SV=1)

HSP 1 Score: 354.8 bits (909), Expect = 2.6e-94
Identity = 171/198 (86.36%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSA APWKSLLL ALESN QIKHS FL LATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAEI WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEICWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 198 AIPCVGRETLWESERINP 216
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of Carg25704 vs. ExPASy TrEMBL
Match: A0A1S4DSQ7 (Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=1)

HSP 1 Score: 354.4 bits (908), Expect = 3.4e-94
Identity = 170/198 (85.86%), Postives = 179/198 (90.40%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MGSA APWKSLLL ALESN QIKHS FL LATIG NGRPSNRTVVFR FEEGTDRIHIYT
Sbjct: 1   MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           DSRSRKIEELK CPFAE+ WYFT++WEQFRISGR+EVVDASCLDATKFK+RAEAWSSISP
Sbjct: 61  DSRSRKIEELKNCPFAEVCWYFTDTWEQFRISGRMEVVDASCLDATKFKQRAEAWSSISP 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           KSRMQYLGPSP LP++AEQPAKEP LDS SGPVDAFCLLV DPDQVDYLNLKS ER LF+
Sbjct: 121 KSRMQYLGPSPGLPHLAEQPAKEPFLDSYSGPVDAFCLLVFDPDQVDYLNLKSEERNLFR 180

Query: 198 AIPCVGRETLWESERINP 216
           AIP  G E LWE+ERINP
Sbjct: 181 AIPSFGGEKLWETERINP 198

[Close]

BLAST of Carg25704 vs. TAIR 10
Match: AT2G46580.1 (Pyridoxamine 5'-phosphate oxidase family protein )

HSP 1 Score: 239.2 bits (609), Expect = 3.1e-63
Identity = 111/198 (56.06%), Postives = 150/198 (75.76%), Query Frame = 0


Query: 18  MGSAAAPWKSLLLSALESNAQIKHSTFLHLATIGANGRPSNRTVVFRGFEEGTDRIHIYT 77
           MG+  APWK LL  A+E+N+ + HS+++ LATIG NGRPSNRTVVFRGFEE +DRI I T
Sbjct: 1   MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 78  DSRSRKIEELKTCPFAEISWYFTESWEQFRISGRIEVVDASCLDATKFKKRAEAWSSISP 137
           D RSRKIEELK CPF+E+ WYF+++WEQFRI+GRIEV+DAS  D TK ++R +AW + S 
Sbjct: 61  DLRSRKIEELKHCPFSEMCWYFSDTWEQFRINGRIEVIDASNPDQTKLQQREKAWFANSL 120

Query: 138 KSRMQYLGPSPNLPYIAEQPAKEPVLDSCSGPVDAFCLLVLDPDQVDYLNLKSAERKLFK 197
           +SR+ Y+ P+P  P  +EQ +++  LD  SGPV  +CLL+L+P++VDYLNLK+ +R  F 
Sbjct: 121 RSRLIYVCPTPGSPCNSEQSSQQVKLDPSSGPVPEYCLLLLEPEKVDYLNLKTNQRLFFS 180

Query: 198 AIPCVGRETLWESERINP 216
           ++     E  W SE++NP
Sbjct: 181 SMATGTGEKCWTSEKVNP 198

The following BLAST results are available for this feature:

BLAST of Carg25704 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019079.1	5.7e-120	100.00	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2, partial [Cucurbita argyrosperma ...	[more]
XP_022924710.1	5.7e-120	100.00	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita moschata] >...	[more]
XP_022980848.1	3.6e-106	96.97	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita maxima]	[more]
XP_023527386.1	6.8e-105	95.96	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Cucurbita pepo subsp....	[more]
XP_022924711.1	6.4e-95	85.12	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Cucurbita moschata]	[more]

BLAST of Carg25704 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZPY1	4.3e-62	56.06	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q7P203	2.5e-09	35.29	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Chromobacterium violaceum (strai...	[more]
Q65QT9	3.1e-07	29.41	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Mannheimia succiniciproducens (s...	[more]
A1TQY3	4.0e-07	36.27	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Acidovorax citrulli (strain AAC0...	[more]
A9AGI1	6.8e-07	34.31	Pyridoxine/pyridoxamine 5'-phosphate oxidase OS=Burkholderia multivorans (strain...	[more]

BLAST of Carg25704 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFU0	2.8e-120	100.00	Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE...	[more]
A0A6J1J0G7	1.7e-106	96.97	Pyridoxal 5'-phosphate synthase OS=Cucurbita maxima OX=3661 GN=LOC111480118 PE=4...	[more]
A0A6J1EA79	3.1e-95	85.12	Pyridoxal 5'-phosphate synthase OS=Cucurbita moschata OX=3662 GN=LOC111432127 PE...	[more]
A0A5A7U3K2	2.6e-94	86.36	Pyridoxal 5'-phosphate synthase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_...	[more]
A0A1S4DSQ7	3.4e-94	85.86	Pyridoxal 5'-phosphate synthase OS=Cucumis melo OX=3656 GN=LOC103483173 PE=4 SV=...	[more]

BLAST of Carg25704 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G46580.1	3.1e-63	56.06	Pyridoxamine 5'-phosphate oxidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024624	Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domain	PFAM	PF12766	Pyridox_oxase_2	coord: 23..115 e-value: 9.1E-27 score: 93.6
IPR012349	FMN-binding split barrel	GENE3D	2.30.110.10		coord: 20..215 e-value: 2.9E-54 score: 185.9
IPR024015	Pyridoxamine 5'-phosphate oxidase, probable FMN-dependent, Alr4036 family	TIGRFAM	TIGR04026	TIGR04026	coord: 23..215 e-value: 1.4E-47 score: 160.0
IPR000659	Pyridoxamine 5'-phosphate oxidase	PANTHER	PTHR10851	PYRIDOXINE-5-PHOSPHATE OXIDASE	coord: 19..215
None	No IPR available	PANTHER	PTHR10851:SF3	PYRIDOXINE/PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2	coord: 19..215
None	No IPR available	SUPERFAMILY	50475	FMN-binding split barrel	coord: 18..215

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25704-RA	Carg25704-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042823	pyridoxal phosphate biosynthetic process
biological_process	GO:0008615	pyridoxine biosynthetic process
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0004733	pyridoxamine-phosphate oxidase activity