Carg25699 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25699
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	aspartyl protease family protein 2-like
Location	Carg_Chr14: 14691667 .. 14693091 (-)
RNA-Seq Expression	Carg25699
Synteny	Carg25699

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGCGAAAACCAGTCCATTTACCTTTATCTTCGTCCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTCCTTAGCCCCGGAATCCAATGAGGATTCCGACTCCTTCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCGTTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGTCTCTCCAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGTGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACATGGTGCTCGACACCGGCAGTGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCTTTCTCCAAGGTTCTCTGCCGTACGCCGCTCTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGGACCAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTGTTCGTTGGTGCGGCAGGACTTTTAGGGCTGGGCCGGGGTGGGTTGTCGTTTCCTTCGCAAACCGGCCGGACTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAACGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTGCGTGACGCCTTCCGTGCCGGAGCTTCAAGTTTGAAGTCGGCGGCGGAGTTTTCTCTTTTCGACACATGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGACGTGTCGTTACCAGCGTCCAACTATCTGATCCCGGTCGACGGAAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTAGGATTCTCTCCTCGTGGTTGCGCCTAA

mRNA sequence

ATGGTGGCGAAAACCAGTCCATTTACCTTTATCTTCGTCCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTCCTTAGCCCCGGAATCCAATGAGGATTCCGACTCCTTCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCGTTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGTCTCTCCAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGTGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACATGGTGCTCGACACCGGCAGTGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCTTTCTCCAAGGTTCTCTGCCGTACGCCGCTCTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGGACCAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTGTTCGTTGGTGCGGCAGGACTTTTAGGGCTGGGCCGGGGTGGGTTGTCGTTTCCTTCGCAAACCGGCCGGACTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAACGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTGCGTGACGCCTTCCGTGCCGGAGCTTCAAGTTTGAAGTCGGCGGCGGAGTTTTCTCTTTTCGACACATGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGACGTGTCGTTACCAGCGTCCAACTATCTGATCCCGGTCGACGGAAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTAGGATTCTCTCCTCGTGGTTGCGCCTAA

Coding sequence (CDS)

ATGGTGGCGAAAACCAGTCCATTTACCTTTATCTTCGTCCTTCTCACTCTTCTCTCTCTCTCCACCGCCTTCTCCGATTTCCAAACCCTAGTCCCCAGACCTCTTCCCACTTCACCTTCCTCCTTAGCCCCGGAATCCAATGAGGATTCCGACTCCTTCTTCTCATCTGAGGCCACAGAATCGGAGCCTGGTTTAGCGTTGCACCTTCACCATTTGGACTCCCTCTCTCTCAGCCGAACGCCGGAGGAGCTCTTCCATCTCCGCCTTCAAAGGGACGCTCTCCGAGTCAACAAGCTCAGTTTACTTGCTGCTGTCTCTCCAAATGTGAGCCGAGCGAGTGGGACTGGGTTCAGTAGCTCCGTGATCTCCGGACTCGCTCAGGGCAGTGGTGAGTACTTCACGCGCATCGGCGTCGGCACTCCGCCCAGGTATGTTTACATGGTGCTCGACACCGGCAGTGACATAGTTTGGCTACAGTGCGCTCCTTGCAAGAATTGCTACTCTCAGACCGACCCGGTTTTCGACCCGGTTAAGTCTGGATCTTTCTCCAAGGTTCTCTGCCGTACGCCGCTCTGCGGCCGGCTCGAATCTCCGGGGTGCAACCAGCGGCAGACGTGTCTCTACCAAGTTTCTTACGGCGACGGTTCCTACACCACCGGCGAGTTCGTCACCGAAACCTTGACCTTCCGGCGGACCAAAGTGGAGCGCGTAGCCCTAGGTTGCGGCCACGATAATGAGGGCTTGTTCGTTGGTGCGGCAGGACTTTTAGGGCTGGGCCGGGGTGGGTTGTCGTTTCCTTCGCAAACCGGCCGGACTTTCAACCAGAAATTCTCCTACTGCTTGGTGGACCGGTCCGCCTCTTCCAAACCGTCCTCCGTCGTGTTCGGCAACTCCGCCGTGTCTCGAACCGCCCGGTTCACTCCTCTCCTCACAAACCCCAGACTGGATACGTTTTACTACGTCGAACTTCTAGGGATCAGCGTTGGAGGTAGGCCCGTCTCCGGCATCTCCCCTTTACATTTCAAGCTCGATTCGACCGGTAACGGCGGAGTCATCATCGATTGCGGTACTTCTGTGACTCGGTTGAACCGACCGGCGTACATTGCCCTGCGTGACGCCTTCCGTGCCGGAGCTTCAAGTTTGAAGTCGGCGGCGGAGTTTTCTCTTTTCGACACATGCTACGACCTGTCCGGGAAGACGACGGTGAAGGTCCCAACGGTGGTGCTGCATTTCAGAAACGCCGACGTGTCGTTACCAGCGTCCAACTATCTGATCCCGGTCGACGGAAGCGGGCGATTCTGCTTCGCCTTCGCCGGAACGACCAGTGGGCTGTCGATCATCGGCAACATTCAGCAGCAAGGATTTCGGGTGGTGTACGATTTGGCAGGTTCTCGGGTAGGATTCTCTCCTCGTGGTTGCGCCTAA

Protein sequence

MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA

Homology

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BLAST of Carg25699 vs. NCBI nr
Match: XP_022924595.1 (aspartyl protease family protein 2-like [Cucurbita moschata] >KAG6582675.1 Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019074.1 Aspartyl protease family protein 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 940.3 bits (2429), Expect = 6.6e-270
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE
Sbjct: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

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BLAST of Carg25699 vs. NCBI nr
Match: XP_023527618.1 (aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 929.9 bits (2402), Expect = 9.0e-267
Identity = 469/474 (98.95%), Postives = 470/474 (99.16%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           MVAKTSPF FIF LLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESN+DSDSFFSSEATE
Sbjct: 28  MVAKTSPFPFIFFLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNQDSDSFFSSEATE 87

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAA SPNVSRASGTGFSSS
Sbjct: 88  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAGSPNVSRASGTGFSSS 147

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG
Sbjct: 148 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 207

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 208 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 267

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPS VVFGNSAV
Sbjct: 268 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSFVVFGNSAV 327

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT
Sbjct: 328 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 387

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA
Sbjct: 388 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 447

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 448 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 501

[Close]

BLAST of Carg25699 vs. NCBI nr
Match: XP_022980038.1 (aspartyl protease family protein 2-like [Cucurbita maxima])

HSP 1 Score: 911.8 bits (2355), Expect = 2.5e-261
Identity = 463/474 (97.68%), Postives = 464/474 (97.89%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           MVAKTSPF FIF LLTLL LSTAFSDFQTLVPRPLPTSPS LAPES E SDS FSSEATE
Sbjct: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDS-FSSEATE 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAA S NVSRASGTGFSSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPRYVY+VLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGR FNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

[Close]

BLAST of Carg25699 vs. NCBI nr
Match: XP_038877929.1 (aspartyl protease family protein 2-like [Benincasa hispida])

HSP 1 Score: 839.7 bits (2168), Expect = 1.2e-239
Identity = 427/474 (90.08%), Postives = 440/474 (92.83%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           M A T  F+FIF LLTLLSLSTA SDFQTL+P  LP+SPS L      DS+SF SS+ TE
Sbjct: 1   MEAMTKLFSFIFFLLTLLSLSTAISDFQTLIPTSLPSSPSFLP----SDSESFISSDTTE 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           S+ GL LHLHHLD+LSL+RTPEELFHLRLQRDALRV KLS L   S NVS+ SGTGFSSS
Sbjct: 61  SDLGLTLHLHHLDALSLNRTPEELFHLRLQRDALRVKKLSSLHDSSQNVSQTSGTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SF+KVLCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SFAKVLCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGN+AV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNAAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 470

[Close]

BLAST of Carg25699 vs. NCBI nr
Match: XP_008437888.1 (PREDICTED: aspartyl protease family protein 2 [Cucumis melo] >KAA0048886.1 protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa] >TYK20836.1 protein ASPARTIC PROTEASE IN GUARD CELL 2-like [Cucumis melo var. makuwa])

HSP 1 Score: 827.4 bits (2136), Expect = 6.3e-236
Identity = 422/469 (89.98%), Postives = 437/469 (93.18%), Query Frame = 0


Query: 7   PFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEPGLA 66
           PF F F+LL +LSLSTAFSDFQTL+ R LP+SPS L      DS+SF SSEATE+E GL 
Sbjct: 11  PFIF-FLLLAILSLSTAFSDFQTLILRSLPSSPSFLP----SDSNSFLSSEATETELGLE 70

Query: 67  LHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASG-TGFSSSVISGL 126
           LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SG TGFSSSVISGL
Sbjct: 71  LHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSVISGL 130

Query: 127 AQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKV 186
           AQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSGSF+KV
Sbjct: 131 AQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKV 190

Query: 187 LCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDN 246
           LCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALGCGHDN
Sbjct: 191 LCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHDN 250

Query: 247 EGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 306
           EGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR
Sbjct: 251 EGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 310

Query: 307 FTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRP 366
           FTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVTRLN+P
Sbjct: 311 FTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKP 370

Query: 367 AYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLI 426
           AYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPASNYLI
Sbjct: 371 AYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLI 430

Query: 427 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 431 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

[Close]

BLAST of Carg25699 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 632.9 bits (1631), Expect = 3.0e-180
Identity = 331/477 (69.39%), Postives = 381/477 (79.87%), Query Frame = 0


Query: 8   FTFIFVLLTLLSLSTAFSDFQTLVP--RPLP-TSPSSLAPESNEDS---DSFFSSEATES 67
           F+  F  L+L S S +   FQTL P    LP  SP S  P+S+ +S     F S   +ES
Sbjct: 10  FSLCFFFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 69

Query: 68  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSP--NVSRASGT-GFS 127
              + L+L H+D+LS ++TP+ELF  RLQRD+ RV  ++ LAA  P  NV+ A    GFS
Sbjct: 70  SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFS 129

Query: 128 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 187
           SSV+SGL+QGSGEYFTR+GVGTP RYVYMVLDTGSDIVWLQCAPC+ CYSQ+DP+FDP K
Sbjct: 130 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRK 189

Query: 188 SGSFSKVLCRTPLCGRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERV 247
           S +++ + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR +V+ V
Sbjct: 190 SKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGV 249

Query: 248 ALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGN 307
           ALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPSSVVFGN
Sbjct: 250 ALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGN 309

Query: 308 SAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGT 367
           +AVSR ARFTPLL+NP+LDTFYYV LLGISVGG  V G++   FKLD  GNGGVIID GT
Sbjct: 310 AAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGT 369

Query: 368 SVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVS 427
           SVTRL RPAYIA+RDAFR GA +LK A +FSLFDTC+DLS    VKVPTVVLHFR ADVS
Sbjct: 370 SVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVS 429

Query: 428 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           LPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GCA
Sbjct: 430 LPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Carg25699 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 2.5e-110
Identity = 224/476 (47.06%), Postives = 297/476 (62.39%), Query Frame = 0


Query: 7   PFTFIFVLLTL---LSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEP 66
           P  F F+ L L    S S +F DFQ +     P + ++  P+ N   ++ FS E++    
Sbjct: 4   PLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN---NTHFSDESSSKYT 63

Query: 67  GLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNV-----SRASGTGFS 126
              LH     S++  R      H R++RD  RV+  ++L  +S  V     SR     F 
Sbjct: 64  LRLLHRDRFPSVTY-RNHHHRLHARMRRDTDRVS--AILRRISGKVIPSSDSRYEVNDFG 123

Query: 127 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 186
           S ++SG+ QGSGEYF RIGVG+PPR  YMV+D+GSD+VW+QC PCK CY Q+DPVFDP K
Sbjct: 124 SDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAK 183

Query: 187 SGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 246
           SGS++ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA
Sbjct: 184 SGSYTGVSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVA 243

Query: 247 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNS 306
           +GCGH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  
Sbjct: 244 MGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGRE 303

Query: 307 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 366
           A+   A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+
Sbjct: 304 ALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTA 363

Query: 367 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADV-S 426
           VTRL   AY+A RD F++  ++L  A+  S+FDTCYDLSG  +V+VPTV  +F    V +
Sbjct: 364 VTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLT 423

Query: 427 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           LPA N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 424 LPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Carg25699 vs. ExPASy Swiss-Prot
Match: Q9LS40 (Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASPG1 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.0e-108
Identity = 228/476 (47.90%), Postives = 294/476 (61.76%), Query Frame = 0


Query: 17  LLSLSTAFSDFQTLVP-RPLPTSPSSLAPESNEDSDSFFSSEATESEPGLALHLHHLDSL 76
           +L + ++    QT++   P  +S ++  PES  D   F SS        L+L LH  D+ 
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSP------LSLELHSRDTF 96

Query: 77  SLSRTPE--ELFHLRLQRDALRVNKL-------------SLLAAVSPNVSRASGTGFSSS 136
             S+  +   L   RL+RD+ RV  +             S L  V    +R      ++ 
Sbjct: 97  VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTP 156

Query: 137 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 196
           V+SG +QGSGEYF+RIGVGTP + +Y+VLDTGSD+ W+QC PC +CY Q+DPVF+P  S 
Sbjct: 157 VVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSS 216

Query: 197 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVAL 256
           ++  + C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VAL
Sbjct: 217 TYKSLTCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVAL 276

Query: 257 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSA 316
           GCGHDNEGLF GAAGLLGLG G LS  +Q   T    FSYCLVDR  S K SS+ F +  
Sbjct: 277 GCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQ 336

Query: 317 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 376
           +       PLL N ++DTFYYV L G SVGG  V  +    F +D++G+GGVI+DCGT+V
Sbjct: 337 LGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAV 396

Query: 377 TRLNRPAYIALRDAF-RAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNA-DVS 436
           TRL   AY +LRDAF +   +  K ++  SLFDTCYD S  +TVKVPTV  HF     + 
Sbjct: 397 TRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLD 456

Query: 437 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           LPA NYLIPVD SG FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 LPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

[Close]

BLAST of Carg25699 vs. ExPASy Swiss-Prot
Match: Q9LEW3 (Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 4.9e-74
Identity = 180/440 (40.91%), Postives = 245/440 (55.68%), Query Frame = 0


Query: 40  SSLAPESNEDSDSFFSSEATESEPGL-ALHLHHLDSLSLSRTPEELFHLRLQRDALRVNK 99
           SSL P S   S    SS+A+ ++  L  +H+H   S  LS          ++RD  RV  
Sbjct: 43  SSLFPSS---SSCVPSSKASNTKSSLRVVHMHGACS-HLSSDARVDHDEIIRRDQARVES 102

Query: 100 L-SLLAAVSPN-VSRASGTGFSSSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIV 159
           + S L+  S N VS A  T   +   SG+  GSG Y   IG+GTP   + +V DTGSD+ 
Sbjct: 103 IYSKLSKNSANEVSEAKSTELPAK--SGITLGSGNYIVTIGIGTPKHDLSLVFDTGSDLT 162

Query: 160 WLQCAPC-KNCYSQTDPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDG 219
           W QC PC  +CYSQ +P F+P  S ++  V C +P+C   ES  C+    C+Y + YGD 
Sbjct: 163 WTQCEPCLGSCYSQKEPKFNPSSSSTYQNVSCSSPMCEDAES--CS-ASNCVYSIVYGDK 222

Query: 220 SYTTGEFVTETLTFRRTKV-ERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFN 279
           S+T G    E  T   + V E V  GCG +N+GLF G AGLLGLG G LS P+QT  T+N
Sbjct: 223 SFTQGFLAKEKFTLTNSDVLEDVYFGCGENNQGLFDGVAGLLGLGPGKLSLPAQTTTTYN 282

Query: 280 QKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVS 339
             FSYCL   +++S    + FG++ +S + +FTP+ + P     Y ++++GISVG + ++
Sbjct: 283 NIFSYCLPSFTSNS-TGHLTFGSAGISESVKFTPISSFPSAFN-YGIDIIGISVGDKELA 342

Query: 340 GISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCY 399
            I+P  F  +     G IID GT  TRL    Y  LR  F+   SS KS + + LFDTCY
Sbjct: 343 -ITPNSFSTE-----GAIIDSGTVFTRLPTKVYAELRSVFKEKMSSYKSTSGYGLFDTCY 402

Query: 400 DLSGKTTVKVPTVVLHFRNAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQ 459
           D +G  TV  PT+   F  +  V L  S   +P+  S + C AFAG     +I GN+QQ 
Sbjct: 403 DFTGLDTVTYPTIAFSFAGSTVVELDGSGISLPIKIS-QVCLAFAGNDDLPAIFGNVQQT 462

Query: 460 GFRVVYDLAGSRVGFSPRGC 474
              VVYD+AG RVGF+P GC
Sbjct: 463 TLDVVYDVAGGRVGFAPNGC 464

[Close]

BLAST of Carg25699 vs. ExPASy Swiss-Prot
Match: Q8S9J6 (Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At5g10770 PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.4e-73
Identity = 177/451 (39.25%), Postives = 246/451 (54.55%), Query Frame = 0


Query: 40  SSLAPESNEDSDSFFSSEATESEPGLALHLHHLDSLSLSR----TPEELFHLRLQRDALR 99
           SSL P S+  S    S  A+ ++  L +   H     L+     +P+ +  LRL  D  R
Sbjct: 39  SSLLPSSS--SSCVLSPRASTTKSSLHVTHRHGTCSRLNNGKATSPDHVEILRL--DQAR 98

Query: 100 VN----KLSLLAAVSPNVSRASGTGFSSSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDT 159
           VN    KLS   A + +VS +  T   +    G   GSG Y   +G+GTP   + ++ DT
Sbjct: 99  VNSIHSKLSKKLA-TDHVSESKSTDLPAK--DGSTLGSGNYIVTVGLGTPKNDLSLIFDT 158

Query: 160 GSDIVWLQCAPC-KNCYSQTDPVFDPVKSGSFSKVLCRTPLCGRLES----PGCNQRQTC 219
           GSD+ W QC PC + CY Q +P+F+P KS S+  V C +  CG L S     G      C
Sbjct: 159 GSDLTWTQCQPCVRTCYDQKEPIFNPSKSTSYYNVSCSSAACGSLSSATGNAGSCSASNC 218

Query: 220 LYQVSYGDGSYTTGEFVTETLTFRRTKV-ERVALGCGHDNEGLFVGAAGLLGLGRGGLSF 279
           +Y + YGD S++ G    E  T   + V + V  GCG +N+GLF G AGLLGLGR  LSF
Sbjct: 219 IYGIQYGDQSFSVGFLAKEKFTLTNSDVFDGVYFGCGENNQGLFTGVAGLLGLGRDKLSF 278

Query: 280 PSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLG 339
           PSQT   +N+ FSYCL   S++S    + FG++ +SR+ +FTP+ T     +FY + ++ 
Sbjct: 279 PSQTATAYNKIFSYCL--PSSASYTGHLTFGSAGISRSVKFTPISTITDGTSFYGLNIVA 338

Query: 340 ISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAA 399
           I+VGG+ +    P+   + ST   G +ID GT +TRL   AY ALR +F+A  S   + +
Sbjct: 339 ITVGGQKL----PIPSTVFST--PGALIDSGTVITRLPPKAYAALRSSFKAKMSKYPTTS 398

Query: 400 EFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLIPVDGSGRFCFAFAGTT--SG 459
             S+ DTC+DLSG  TV +P V   F    V    S  +  V    + C AFAG +  S 
Sbjct: 399 GVSILDTCFDLSGFKTVTIPKVAFSFSGGAVVELGSKGIFYVFKISQVCLAFAGNSDDSN 458

Query: 460 LSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
            +I GN+QQQ   VVYD AG RVGF+P GC+
Sbjct: 459 AAIFGNVQQQTLEVVYDGAGGRVGFAPNGCS 474

[Close]

BLAST of Carg25699 vs. ExPASy TrEMBL
Match: A0A6J1EFG4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111432036 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.2e-270
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE
Sbjct: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of Carg25699 vs. ExPASy TrEMBL
Match: A0A6J1IY26 (aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479557 PE=3 SV=1)

HSP 1 Score: 911.8 bits (2355), Expect = 1.2e-261
Identity = 463/474 (97.68%), Postives = 464/474 (97.89%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           MVAKTSPF FIF LLTLL LSTAFSDFQTLVPRPLPTSPS LAPES E SDS FSSEATE
Sbjct: 1   MVAKTSPFPFIFFLLTLLPLSTAFSDFQTLVPRPLPTSPSFLAPESTEGSDS-FSSEATE 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGTGFSSS 120
           SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAA S NVSRASGTGFSSS
Sbjct: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAASRNVSRASGTGFSSS 120

Query: 121 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180
           VISGLAQGSGEYFTRIGVGTPPRYVY+VLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG
Sbjct: 121 VISGLAQGSGEYFTRIGVGTPPRYVYLVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 180

Query: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240
           SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG
Sbjct: 181 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALG 240

Query: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAV 300
           CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGR FNQKFSYCLVDRSASSKPSSVVFGNSAV
Sbjct: 241 CGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRAFNQKFSYCLVDRSASSKPSSVVFGNSAV 300

Query: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360
           SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT
Sbjct: 301 SRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVT 360

Query: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420
           RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA
Sbjct: 361 RLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPA 420

Query: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 SNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 473

[Close]

BLAST of Carg25699 vs. ExPASy TrEMBL
Match: A0A5A7U3R3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold291G00990 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.0e-236
Identity = 422/469 (89.98%), Postives = 437/469 (93.18%), Query Frame = 0


Query: 7   PFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEPGLA 66
           PF F F+LL +LSLSTAFSDFQTL+ R LP+SPS L      DS+SF SSEATE+E GL 
Sbjct: 11  PFIF-FLLLAILSLSTAFSDFQTLILRSLPSSPSFLP----SDSNSFLSSEATETELGLE 70

Query: 67  LHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASG-TGFSSSVISGL 126
           LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SG TGFSSSVISGL
Sbjct: 71  LHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSVISGL 130

Query: 127 AQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKV 186
           AQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSGSF+KV
Sbjct: 131 AQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKV 190

Query: 187 LCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDN 246
           LCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALGCGHDN
Sbjct: 191 LCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHDN 250

Query: 247 EGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 306
           EGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR
Sbjct: 251 EGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 310

Query: 307 FTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRP 366
           FTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVTRLN+P
Sbjct: 311 FTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKP 370

Query: 367 AYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLI 426
           AYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPASNYLI
Sbjct: 371 AYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLI 430

Query: 427 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 431 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

[Close]

BLAST of Carg25699 vs. ExPASy TrEMBL
Match: A0A1S3AV66 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103483183 PE=3 SV=1)

HSP 1 Score: 827.4 bits (2136), Expect = 3.0e-236
Identity = 422/469 (89.98%), Postives = 437/469 (93.18%), Query Frame = 0


Query: 7   PFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEPGLA 66
           PF F F+LL +LSLSTAFSDFQTL+ R LP+SPS L      DS+SF SSEATE+E GL 
Sbjct: 11  PFIF-FLLLAILSLSTAFSDFQTLILRSLPSSPSFLP----SDSNSFLSSEATETELGLE 70

Query: 67  LHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASG-TGFSSSVISGL 126
           LHLHHLD+LS +RTPEELFHLRLQRDA+RV KLS L A S N+SR SG TGFSSSVISGL
Sbjct: 71  LHLHHLDALSFNRTPEELFHLRLQRDAIRVKKLSSLGATSRNLSRPSGTTGFSSSVISGL 130

Query: 127 AQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKV 186
           AQGSGEYFTRIGVGTPP+YVYMVLDTGSDIVWLQCAPCKNCYSQTDPVF+PVKSGSF+KV
Sbjct: 131 AQGSGEYFTRIGVGTPPKYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFNPVKSGSFAKV 190

Query: 187 LCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCGHDN 246
           LCRTPLC RLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE+VALGCGHDN
Sbjct: 191 LCRTPLCRRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVEQVALGCGHDN 250

Query: 247 EGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 306
           EGLFVGAAGLLGLGRGGLSFPSQ GRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR
Sbjct: 251 EGLFVGAAGLLGLGRGGLSFPSQAGRTFNQKFSYCLVDRSASSKPSSVVFGNSAVSRTAR 310

Query: 307 FTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSVTRLNRP 366
           FTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD TGNGGVIIDCGTSVTRLN+P
Sbjct: 311 FTPLLTNPRLDTFYYVELLGISVGGTPVSGISSSHFKLDRTGNGGVIIDCGTSVTRLNKP 370

Query: 367 AYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSLPASNYLI 426
           AYIALRDAFRAGASSLKSA EFSLFDTCYDLSGKTTVKVPTVVLHFR ADVSLPASNYLI
Sbjct: 371 AYIALRDAFRAGASSLKSAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGADVSLPASNYLI 430

Query: 427 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLA SRVGFSPRGCA
Sbjct: 431 PVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLASSRVGFSPRGCA 474

[Close]

BLAST of Carg25699 vs. ExPASy TrEMBL
Match: A0A6J1G0Y1 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111449669 PE=3 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 5.7e-235
Identity = 422/479 (88.10%), Postives = 439/479 (91.65%), Query Frame = 0


Query: 1   MVAKTSPFTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATE 60
           M AKTS F FI  LLTLLSLSTAFSDFQTL+P+ LP SPS L+PES  DS+SF SSEA  
Sbjct: 1   MEAKTSAFPFIGFLLTLLSLSTAFSDFQTLIPKSLPASPSLLSPESATDSESFISSEA-- 60

Query: 61  SEPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASG-----T 120
              GL L LHHLD+LSL+RTPEELFHLRLQRDALRV KLS L+  S N+S+ASG     T
Sbjct: 61  ---GLELQLHHLDALSLNRTPEELFHLRLQRDALRVTKLSSLSGGSQNLSQASGTSHGTT 120

Query: 121 GFSSSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFD 180
           GFSSSVISGLAQGSGEYFTRIGVGTPP+Y+YMVLDTGSDIVWLQCAPCKNCYSQTDPVF+
Sbjct: 121 GFSSSVISGLAQGSGEYFTRIGVGTPPKYIYMVLDTGSDIVWLQCAPCKNCYSQTDPVFN 180

Query: 181 PVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE 240
           PVKS S+SKVLCRTPLC RLESPGCNQ+QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE
Sbjct: 181 PVKSRSYSKVLCRTPLCLRLESPGCNQKQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVE 240

Query: 241 RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVF 300
           RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQ GR+FNQKFSYCLVDRSASSKPSSVVF
Sbjct: 241 RVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQAGRSFNQKFSYCLVDRSASSKPSSVVF 300

Query: 301 GNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDC 360
           G+SAVSRTARFTPLLTNPRLDTFYYVELLGISVGG PVSGIS  HFKLD  GNGGVIIDC
Sbjct: 301 GDSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGTPVSGISASHFKLDPNGNGGVIIDC 360

Query: 361 GTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNAD 420
           GTSVTRLNRPAYIALRDAFRAGASSLK A EFSLFDTCYDLSGKTTVKVPTVVLHFR AD
Sbjct: 361 GTSVTRLNRPAYIALRDAFRAGASSLKLAPEFSLFDTCYDLSGKTTVKVPTVVLHFRGAD 420

Query: 421 VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           VSLPASNYLIPVD +GRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA
Sbjct: 421 VSLPASNYLIPVDDNGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 474

[Close]

BLAST of Carg25699 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 632.9 bits (1631), Expect = 2.1e-181
Identity = 331/477 (69.39%), Postives = 381/477 (79.87%), Query Frame = 0


Query: 8   FTFIFVLLTLLSLSTAFSDFQTLVP--RPLP-TSPSSLAPESNEDS---DSFFSSEATES 67
           F+  F  L+L S S +   FQTL P    LP  SP S  P+S+ +S     F S   +ES
Sbjct: 10  FSLCFFFLSLPSFS-SLPSFQTLFPNSHSLPCASPVSFQPDSDSESLLESEFESGSDSES 69

Query: 68  EPGLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSP--NVSRASGT-GFS 127
              + L+L H+D+LS ++TP+ELF  RLQRD+ RV  ++ LAA  P  NV+ A    GFS
Sbjct: 70  SSSITLNLDHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFS 129

Query: 128 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 187
           SSV+SGL+QGSGEYFTR+GVGTP RYVYMVLDTGSDIVWLQCAPC+ CYSQ+DP+FDP K
Sbjct: 130 SSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRCYSQSDPIFDPRK 189

Query: 188 SGSFSKVLCRTPLCGRLESPGCN-QRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERV 247
           S +++ + C +P C RL+S GCN +R+TCLYQVSYGDGS+T G+F TETLTFRR +V+ V
Sbjct: 190 SKTYATIPCSSPHCRRLDSAGCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFRRNRVKGV 249

Query: 248 ALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGN 307
           ALGCGHDNEGLFVGAAGLLGLG+G LSFP QTG  FNQKFSYCLVDRSASSKPSSVVFGN
Sbjct: 250 ALGCGHDNEGLFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGN 309

Query: 308 SAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGT 367
           +AVSR ARFTPLL+NP+LDTFYYV LLGISVGG  V G++   FKLD  GNGGVIID GT
Sbjct: 310 AAVSRIARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGT 369

Query: 368 SVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVS 427
           SVTRL RPAYIA+RDAFR GA +LK A +FSLFDTC+DLS    VKVPTVVLHFR ADVS
Sbjct: 370 SVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVS 429

Query: 428 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGCA 475
           LPA+NYLIPVD +G+FCFAFAGT  GLSIIGNIQQQGFRVVYDLA SRVGF+P GCA
Sbjct: 430 LPATNYLIPVDTNGKFCFAFAGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Carg25699 vs. TAIR 10
Match: AT3G61820.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 577.4 bits (1487), Expect = 1.1e-164
Identity = 306/475 (64.42%), Postives = 357/475 (75.16%), Query Frame = 0


Query: 13  VLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEPGLALHLHHL 72
           V   L   S+A S +QTLV   LP+S +   PES   +D   S    ES   L++HL H+
Sbjct: 13  VFAVLFFTSSASSQYQTLVVNTLPSSATLSWPESESLTDESLS----ESTTSLSVHLSHV 72

Query: 73  DSLS--LSRTPEELFHLRLQRDALRVNKLSLLAAVSPNVSRASGT-----GFSSSVISGL 132
           D+LS     +P +LF+LRLQRD+LRV  ++ LAAVS   +    T     GFS +VISGL
Sbjct: 73  DALSSFSDASPADLFNLRLQRDSLRVKSITSLAAVSTGRNATKRTPRTAGGFSGAVISGL 132

Query: 133 AQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSGSFSKV 192
           +QGSGEYF R+GVGTP   VYMVLDTGSD+VWLQC+PCK CY+QTD +FDP KS +F+ V
Sbjct: 133 SQGSGEYFMRLGVGTPATNVYMVLDTGSDVVWLQCSPCKACYNQTDAIFDPKKSKTFATV 192

Query: 193 LCRTPLCGRL-ESPGCNQR--QTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVALGCG 252
            C + LC RL +S  C  R  +TCLYQVSYGDGS+T G+F TETLTF   +V+ V LGCG
Sbjct: 193 PCGSRLCRRLDDSSECVTRRSKTCLYQVSYGDGSFTEGDFSTETLTFHGARVDHVPLGCG 252

Query: 253 HDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDR----SASSKPSSVVFGNS 312
           HDNEGLFVGAAGLLGLGRGGLSFPSQT   +N KFSYCLVDR    S+S  PS++VFGN+
Sbjct: 253 HDNEGLFVGAAGLLGLGRGGLSFPSQTKNRYNGKFSYCLVDRTSSGSSSKPPSTIVFGNA 312

Query: 313 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 372
           AV +T+ FTPLLTNP+LDTFYY++LLGISVGG  V G+S   FKLD+TGNGGVIID GTS
Sbjct: 313 AVPKTSVFTPLLTNPKLDTFYYLQLLGISVGGSRVPGVSESQFKLDATGNGGVIIDSGTS 372

Query: 373 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADVSL 432
           VTRL +PAY+ALRDAFR GA+ LK A  +SLFDTC+DLSG TTVKVPTVV HF   +VSL
Sbjct: 373 VTRLTQPAYVALRDAFRLGATKLKRAPSYSLFDTCFDLSGMTTVKVPTVVFHFGGGEVSL 432

Query: 433 PASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           PASNYLIPV+  GRFCFAFAGT   LSIIGNIQQQGFRV YDL GSRVGF  R C
Sbjct: 433 PASNYLIPVNTEGRFCFAFAGTMGSLSIIGNIQQQGFRVAYDLVGSRVGFLSRAC 483

[Close]

BLAST of Carg25699 vs. TAIR 10
Match: AT3G20015.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.6 bits (1028), Expect = 1.8e-111
Identity = 224/476 (47.06%), Postives = 297/476 (62.39%), Query Frame = 0


Query: 7   PFTFIFVLLTL---LSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSSEATESEP 66
           P  F F+ L L    S S +F DFQ +     P + ++  P+ N   ++ FS E++    
Sbjct: 4   PLFFFFLHLHLHLSSSSSISFPDFQIIDVLQPPLTVTATLPDFN---NTHFSDESSSKYT 63

Query: 67  GLALHLHHLDSLSLSRTPEELFHLRLQRDALRVNKLSLLAAVSPNV-----SRASGTGFS 126
              LH     S++  R      H R++RD  RV+  ++L  +S  V     SR     F 
Sbjct: 64  LRLLHRDRFPSVTY-RNHHHRLHARMRRDTDRVS--AILRRISGKVIPSSDSRYEVNDFG 123

Query: 127 SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVK 186
           S ++SG+ QGSGEYF RIGVG+PPR  YMV+D+GSD+VW+QC PCK CY Q+DPVFDP K
Sbjct: 124 SDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLCYKQSDPVFDPAK 183

Query: 187 SGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRTKVERVA 246
           SGS++ V C + +C R+E+ GC+    C Y+V YGDGSYT G    ETLTF +T V  VA
Sbjct: 184 SGSYTGVSCGSSVCDRIENSGCHS-GGCRYEVMYGDGSYTKGTLALETLTFAKTVVRNVA 243

Query: 247 LGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNS 306
           +GCGH N G+F+GAAGLLG+G G +SF  Q        F YCLV R   S   S+VFG  
Sbjct: 244 MGCGHRNRGMFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLVSRGTDS-TGSLVFGRE 303

Query: 307 AVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTS 366
           A+   A + PL+ NPR  +FYYV L G+ VGG  +  +    F L  TG+GGV++D GT+
Sbjct: 304 ALPVGASWVPLVRNPRAPSFYYVGLKGLGVGGVRIP-LPDGVFDLTETGDGGVVMDTGTA 363

Query: 367 VTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNADV-S 426
           VTRL   AY+A RD F++  ++L  A+  S+FDTCYDLSG  +V+VPTV  +F    V +
Sbjct: 364 VTRLPTAAYVAFRDGFKSQTANLPRASGVSIFDTCYDLSGFVSVRVPTVSFYFTEGPVLT 423

Query: 427 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           LPA N+L+PVD SG +CFAFA + +GLSIIGNIQQ+G +V +D A   VGF P  C
Sbjct: 424 LPARNFLMPVDDSGTYCFAFAASPTGLSIIGNIQQEGIQVSFDGANGFVGFGPNVC 470

[Close]

BLAST of Carg25699 vs. TAIR 10
Match: AT1G25510.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.3e-111
Identity = 240/489 (49.08%), Postives = 299/489 (61.15%), Query Frame = 0


Query: 8   FTFIFVLLTLLSLSTAFSDFQTLVPRPLPTSPSSLAPESNEDSDSFFSS-------EATE 67
           ++F F +  L S S+ FS    ++P    T+ S L    +     + SS       E T 
Sbjct: 5   YSFFFFIFFLTSHSSVFS---RILPETSTTTTSILNVADSIHRTKYTSSFRLNQQEEQTH 64

Query: 68  S-EPGLALHLHHLDSLSLSRTPE--ELFHLRLQRDALRVNKL-SLLAAVSPNVSRASGTG 127
           S     +L LH   S+  +   +   L   RL RD  RV  L + L     N+S+A    
Sbjct: 65  SASSSFSLQLHSRVSVRGTEHSDYKSLTLARLNRDTARVKSLITRLDLAINNISKADLKP 124

Query: 128 FS-----------SSVISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKN 187
            S           + +ISG  QGSGEYFTR+G+G P R VYMVLDTGSD+ WLQC PC +
Sbjct: 125 ISTMYTTEEQDIEAPLISGTTQGSGEYFTRVGIGKPAREVYMVLDTGSDVNWLQCTPCAD 184

Query: 188 CYSQTDPVFDPVKSGSFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTE 247
           CY QT+P+F+P  S S+  + C TP C  LE   C +  TCLY+VSYGDGSYT G+F TE
Sbjct: 185 CYHQTEPIFEPSSSSSYEPLSCDTPQCNALEVSEC-RNATCLYEVSYGDGSYTVGDFATE 244

Query: 248 TLTFRRTKVERVALGCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRS 307
           TLT   T V+ VA+GCGH NEGLFVGAAGLLGLG G L+ PSQ   T    FSYCLVDR 
Sbjct: 245 TLTIGSTLVQNVAVGCGHSNEGLFVGAAGLLGLGGGLLALPSQLNTT---SFSYCLVDRD 304

Query: 308 ASSKPSSVVFGNSAVSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDS 367
           + S  S+V FG S +S  A   PLL N +LDTFYY+ L GISVGG  +  I    F++D 
Sbjct: 305 SDS-ASTVDFGTS-LSPDAVVAPLLRNHQLDTFYYLGLTGISVGGELLQ-IPQSSFEMDE 364

Query: 368 TGNGGVIIDCGTSVTRLNRPAYIALRDAFRAGASSLKSAAEFSLFDTCYDLSGKTTVKVP 427
           +G+GG+IID GT+VTRL    Y +LRD+F  G   L+ AA  ++FDTCY+LS KTTV+VP
Sbjct: 365 SGSGGIIIDSGTAVTRLQTEIYNSLRDSFVKGTLDLEKAAGVAMFDTCYNLSAKTTVEVP 424

Query: 428 TVVLHFRNAD-VSLPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGS 474
           TV  HF     ++LPA NY+IPVD  G FC AFA T S L+IIGN+QQQG RV +DLA S
Sbjct: 425 TVAFHFPGGKMLALPAKNYMIPVDSVGTFCLAFAPTASSLAIIGNVQQQGTRVTFDLANS 483

[Close]

BLAST of Carg25699 vs. TAIR 10
Match: AT3G18490.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 393.3 bits (1009), Expect = 2.8e-109
Identity = 228/476 (47.90%), Postives = 294/476 (61.76%), Query Frame = 0


Query: 17  LLSLSTAFSDFQTLVP-RPLPTSPSSLAPESNEDSDSFFSSEATESEPGLALHLHHLDSL 76
           +L + ++    QT++   P  +S ++  PES  D   F SS        L+L LH  D+ 
Sbjct: 37  VLDVVSSLQQTQTILSLDPTRSSLTTTKPESLSDPVFFNSSSP------LSLELHSRDTF 96

Query: 77  SLSRTPE--ELFHLRLQRDALRVNKL-------------SLLAAVSPNVSRASGTGFSSS 136
             S+  +   L   RL+RD+ RV  +             S L  V    +R      ++ 
Sbjct: 97  VASQHKDYKSLTLSRLERDSSRVAGIVAKIRFAVEGVDRSDLKPVYNEDTRYQTEDLTTP 156

Query: 137 VISGLAQGSGEYFTRIGVGTPPRYVYMVLDTGSDIVWLQCAPCKNCYSQTDPVFDPVKSG 196
           V+SG +QGSGEYF+RIGVGTP + +Y+VLDTGSD+ W+QC PC +CY Q+DPVF+P  S 
Sbjct: 157 VVSGASQGSGEYFSRIGVGTPAKEMYLVLDTGSDVNWIQCEPCADCYQQSDPVFNPTSSS 216

Query: 197 SFSKVLCRTPLCGRLESPGCNQRQTCLYQVSYGDGSYTTGEFVTETLTFRRT-KVERVAL 256
           ++  + C  P C  LE+  C   + CLYQVSYGDGS+T GE  T+T+TF  + K+  VAL
Sbjct: 217 TYKSLTCSAPQCSLLETSACRSNK-CLYQVSYGDGSFTVGELATDTVTFGNSGKINNVAL 276

Query: 257 GCGHDNEGLFVGAAGLLGLGRGGLSFPSQTGRTFNQKFSYCLVDRSASSKPSSVVFGNSA 316
           GCGHDNEGLF GAAGLLGLG G LS  +Q   T    FSYCLVDR  S K SS+ F +  
Sbjct: 277 GCGHDNEGLFTGAAGLLGLGGGVLSITNQMKAT---SFSYCLVDRD-SGKSSSLDFNSVQ 336

Query: 317 VSRTARFTPLLTNPRLDTFYYVELLGISVGGRPVSGISPLHFKLDSTGNGGVIIDCGTSV 376
           +       PLL N ++DTFYYV L G SVGG  V  +    F +D++G+GGVI+DCGT+V
Sbjct: 337 LGGGDATAPLLRNKKIDTFYYVGLSGFSVGGEKVV-LPDAIFDVDASGSGGVILDCGTAV 396

Query: 377 TRLNRPAYIALRDAF-RAGASSLKSAAEFSLFDTCYDLSGKTTVKVPTVVLHFRNA-DVS 436
           TRL   AY +LRDAF +   +  K ++  SLFDTCYD S  +TVKVPTV  HF     + 
Sbjct: 397 TRLQTQAYNSLRDAFLKLTVNLKKGSSSISLFDTCYDFSSLSTVKVPTVAFHFTGGKSLD 456

Query: 437 LPASNYLIPVDGSGRFCFAFAGTTSGLSIIGNIQQQGFRVVYDLAGSRVGFSPRGC 474
           LPA NYLIPVD SG FCFAFA T+S LSIIGN+QQQG R+ YDL+ + +G S   C
Sbjct: 457 LPAKNYLIPVDDSGTFCFAFAPTSSSLSIIGNVQQQGTRITYDLSKNVIGLSGNKC 500

The following BLAST results are available for this feature:

BLAST of Carg25699 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022924595.1	6.6e-270	100.00	aspartyl protease family protein 2-like [Cucurbita moschata] >KAG6582675.1 Aspar...	[more]
XP_023527618.1	9.0e-267	98.95	aspartyl protease family protein 2-like [Cucurbita pepo subsp. pepo]	[more]
XP_022980038.1	2.5e-261	97.68	aspartyl protease family protein 2-like [Cucurbita maxima]	[more]
XP_038877929.1	1.2e-239	90.08	aspartyl protease family protein 2-like [Benincasa hispida]	[more]
XP_008437888.1	6.3e-236	89.98	PREDICTED: aspartyl protease family protein 2 [Cucumis melo] >KAA0048886.1 prote...	[more]

BLAST of Carg25699 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNJ3	3.0e-180	69.39	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q9LHE3	2.5e-110	47.06	Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LS40	4.0e-108	47.90	Protein ASPARTIC PROTEASE IN GUARD CELL 1 OS=Arabidopsis thaliana OX=3702 GN=ASP...	[more]
Q9LEW3	4.9e-74	40.91	Aspartyl protease AED1 OS=Arabidopsis thaliana OX=3702 GN=AED1 PE=2 SV=1	[more]
Q8S9J6	1.4e-73	39.25	Aspartyl protease family protein At5g10770 OS=Arabidopsis thaliana OX=3702 GN=At...	[more]

BLAST of Carg25699 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EFG4	3.2e-270	100.00	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1IY26	1.2e-261	97.68	aspartyl protease family protein 2-like OS=Cucurbita maxima OX=3661 GN=LOC111479...	[more]
A0A5A7U3R3	3.0e-236	89.98	Protein ASPARTIC PROTEASE IN GUARD CELL 2-like OS=Cucumis melo var. makuwa OX=11...	[more]
A0A1S3AV66	3.0e-236	89.98	aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103483183 PE=3 ...	[more]
A0A6J1G0Y1	5.7e-235	88.10	aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Carg25699 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G01300.1	2.1e-181	69.39	Eukaryotic aspartyl protease family protein	[more]
AT3G61820.1	1.1e-164	64.42	Eukaryotic aspartyl protease family protein	[more]
AT3G20015.1	1.8e-111	47.06	Eukaryotic aspartyl protease family protein	[more]
AT1G25510.1	2.3e-111	49.08	Eukaryotic aspartyl protease family protein	[more]
AT3G18490.1	2.8e-109	47.90	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 445..460 score: 26.83 coord: 351..362 score: 39.97 coord: 138..158 score: 40.7
IPR001461	Aspartic peptidase A1 family	PANTHER	PTHR13683	ASPARTYL PROTEASES	coord: 46..473
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 132..297 e-value: 2.1E-54 score: 184.4
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 113..293 e-value: 6.5E-54 score: 184.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 294..474 e-value: 1.4E-52 score: 180.1
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 125..473
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 318..469 e-value: 8.5E-35 score: 119.9
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 37..56
None	No IPR available	PANTHER	PTHR13683:SF761	ASPARTYL PROTEASE FAMILY PROTEIN 2-LIKE	coord: 46..473
IPR001969	Aspartic peptidase, active site	PROSITE	PS00141	ASP_PROTEASE	coord: 147..158
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 132..469 score: 45.674072
IPR033873	CND41-like	CDD	cd05472	cnd41_like	coord: 131..473 e-value: 1.81394E-137 score: 395.872

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25699-RA	Carg25699-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004190	aspartic-type endopeptidase activity