Carg25678 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25678
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDUF4283 domain-containing protein
LocationCarg_Chr04: 1471665 .. 1473422 (-)
RNA-Seq ExpressionCarg25678
SyntenyCarg25678
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTTCAATCCAAACATTTCCACTTCAGCCGCCATACCGGCGCCGGCAACGACGGAGCGGCCGCCAGTACCATCGGCGCCACCGTCTGTAACCTCACTCCCTCTCAAACAGCTCGGATCAACCAACAGTTCGATCAGTCTCTCATTGTTTGGGTCGTCGGTAAGAAGATTCATCCACGGCAGCTCGCCGTTCGCCTTCGCCGTAATCTTCATCTCGCTGGAGATTTGGACGTCTTCGAGCTAGGGCTTGGATTTTTTGTGCTCAAATTCTCCAACGCTTTAGACTACTACGAAGCCCTTGAAGAGCGTCCATGGTCGATTCCTCACCTTTGCATCTATGTATTCCCATGGATTCCCAATTTCAAGCCCTCCGAGGCCTCGATTCCTTTCGTTGATGTCTGGATTCGGCTCCCGGAGCTCAGTATCGAGTATTATGACAAGGAGGTTTTGGAGAAAATTGCGGAAACCATCGGCGGCCGTCTCGTGAAAATCGATCCAGTAACTGAAACACGAGAGAAATGTATGTATGCTCGTATCTGTATTAGGATGAATTTAGGTTATCCCCTTAATTTGAGCTTCCAATTTGGGAAAAATCCGCAAAAAATTGTGTATGAGGGTCTGGATTTGTTGTGCATTGTCTGTGGATGTGTTGATGATCTGAAACATGATTGTTTGAGCAACCGTTCTAGTTCTTCTGGCTTTGATCCCCATCACCATAGAGCTCGTCCATTGCAGGCCACTGGTTCGAGTTTGAGTTCGAATGCGAATCCATGTTCGAGTTCAAATCTGAATCAGAATCAGAATCCGAGTTCGAGTTTGAGGTCGAATCCGAATCCGAATCTGAGTTCGAGTTCAAATTCAAATTCGAATTTGAAGATGCAGTTGATTCCTTCTAAACCCGCACCAGCATCAGCTCGTGGATCTAGATTCCAAGTTCTTGAGTTGAATTTGAATGAAGAGCCAAGCCTTCCAGTTAGTGAATCTGATAAGGAAGTAAAAGAATCTCCATCGATAACCATGAAAGCTCCTTTGTTAAAACAGACCAATTTGATTCAATCTGTGCCTTTAGCTCCTTGTGTTCTTGAAGATCATCAGTTCAGGACTGAAAAAACCAGCAGCCCCACAACGCTTGCAGTTCAGAACAATGAACCACAACCACCATCATTGGCTATTAAAAGCATAGCTCCCCTGCAACCATCTTCTGCTTTAGAGGCTGGCCTCAAGTTCTATTCCACTGCAATCCAACAATCAACAATACAGAAAGCGATAAACAACACGCCATCCGAACGAATCAGTGTCGATAGTTTGCCGACTATTTACACGATTGATCCAACAATCACAAGCCTTGCAATTGAATTGTTAGAACTGTCAGCAACAACCACAAGATCAAACCAAAACGAGCATGCTATCCACATTGTGCCAACTTCAGAGGCTGTATCAATGTCTGCATCATGTTGTTCTAAGAAAATGTTGTGCTGGAATTTTCGTGCAACAGACAACGCGAAGCTAATGCGAGCATTGAAAGATCTGATTCAATTACACAAGCCATCGATTGTGCTGATCTTTGGCACCAAGATCGGTGGTACTGATGCAGATCATGTCGTTCGGGAGCTCGCTTTCGACGGTTCATATTGTAGAAAGCCTGATGGCTACAGGGGTGGAGCTTGGCTGTTGTTGTCCCAGCAAGACGTGCAAATTGAAGTCAGCTCCTACAGCCCACAGCAGGTTTCTGCATCGGTAATTCTCCCATTCTAA

mRNA sequence

ATGGCGGTTCAATCCAAACATTTCCACTTCAGCCGCCATACCGGCGCCGGCAACGACGGAGCGGCCGCCAGTACCATCGGCGCCACCGTCTGTAACCTCACTCCCTCTCAAACAGCTCGGATCAACCAACAGTTCGATCAGTCTCTCATTGTTTGGGTCGTCGGTAAGAAGATTCATCCACGGCAGCTCGCCGTTCGCCTTCGCCGTAATCTTCATCTCGCTGGAGATTTGGACGTCTTCGAGCTAGGGCTTGGATTTTTTGTGCTCAAATTCTCCAACGCTTTAGACTACTACGAAGCCCTTGAAGAGCGTCCATGGTCGATTCCTCACCTTTGCATCTATGTATTCCCATGGATTCCCAATTTCAAGCCCTCCGAGGCCTCGATTCCTTTCGTTGATGTCTGGATTCGGCTCCCGGAGCTCAGTATCGAGTATTATGACAAGGAGGTTTTGGAGAAAATTGCGGAAACCATCGGCGGCCGTCTCGTGAAAATCGATCCAGTAACTGAAACACGAGAGAAATGTATGTATGCTCGTATCTGTATTAGGATGAATTTAGGTTATCCCCTTAATTTGAGCTTCCAATTTGGGAAAAATCCGCAAAAAATTGTGTATGAGGGTCTGGATTTGTTGTGCATTGTCTGTGGATGTGTTGATGATCTGAAACATGATTGTTTGAGCAACCGTTCTAGTTCTTCTGGCTTTGATCCCCATCACCATAGAGCTCGTCCATTGCAGGCCACTGGTTCGAGTTTGAGTTCGAATGCGAATCCATGTTCGAGTTCAAATCTGAATCAGAATCAGAATCCGAGTTCGAGTTTGAGGTCGAATCCGAATCCGAATCTGAGTTCGAGTTCAAATTCAAATTCGAATTTGAAGATGCAGTTGATTCCTTCTAAACCCGCACCAGCATCAGCTCGTGGATCTAGATTCCAAGTTCTTGAGTTGAATTTGAATGAAGAGCCAAGCCTTCCAGTTAGTGAATCTGATAAGGAAGTAAAAGAATCTCCATCGATAACCATGAAAGCTCCTTTGTTAAAACAGACCAATTTGATTCAATCTGTGCCTTTAGCTCCTTGTGTTCTTGAAGATCATCAGTTCAGGACTGAAAAAACCAGCAGCCCCACAACGCTTGCAGTTCAGAACAATGAACCACAACCACCATCATTGGCTATTAAAAGCATAGCTCCCCTGCAACCATCTTCTGCTTTAGAGGCTGGCCTCAAGTTCTATTCCACTGCAATCCAACAATCAACAATACAGAAAGCGATAAACAACACGCCATCCGAACGAATCAGTGTCGATAGTTTGCCGACTATTTACACGATTGATCCAACAATCACAAGCCTTGCAATTGAATTGTTAGAACTGTCAGCAACAACCACAAGATCAAACCAAAACGAGCATGCTATCCACATTGTGCCAACTTCAGAGGCTGTATCAATGTCTGCATCATGTTGTTCTAAGAAAATGTTGTGCTGGAATTTTCGTGCAACAGACAACGCGAAGCTAATGCGAGCATTGAAAGATCTGATTCAATTACACAAGCCATCGATTGTGCTGATCTTTGGCACCAAGATCGGTGGTACTGATGCAGATCATGTCGTTCGGGAGCTCGCTTTCGACGGTTCATATTGTAGAAAGCCTGATGGCTACAGGGGTGGAGCTTGGCTGTTGTTGTCCCAGCAAGACGTGCAAATTGAAGTCAGCTCCTACAGCCCACAGCAGGTTTCTGCATCGGTAATTCTCCCATTCTAA

Coding sequence (CDS)

ATGGCGGTTCAATCCAAACATTTCCACTTCAGCCGCCATACCGGCGCCGGCAACGACGGAGCGGCCGCCAGTACCATCGGCGCCACCGTCTGTAACCTCACTCCCTCTCAAACAGCTCGGATCAACCAACAGTTCGATCAGTCTCTCATTGTTTGGGTCGTCGGTAAGAAGATTCATCCACGGCAGCTCGCCGTTCGCCTTCGCCGTAATCTTCATCTCGCTGGAGATTTGGACGTCTTCGAGCTAGGGCTTGGATTTTTTGTGCTCAAATTCTCCAACGCTTTAGACTACTACGAAGCCCTTGAAGAGCGTCCATGGTCGATTCCTCACCTTTGCATCTATGTATTCCCATGGATTCCCAATTTCAAGCCCTCCGAGGCCTCGATTCCTTTCGTTGATGTCTGGATTCGGCTCCCGGAGCTCAGTATCGAGTATTATGACAAGGAGGTTTTGGAGAAAATTGCGGAAACCATCGGCGGCCGTCTCGTGAAAATCGATCCAGTAACTGAAACACGAGAGAAATGTATGTATGCTCGTATCTGTATTAGGATGAATTTAGGTTATCCCCTTAATTTGAGCTTCCAATTTGGGAAAAATCCGCAAAAAATTGTGTATGAGGGTCTGGATTTGTTGTGCATTGTCTGTGGATGTGTTGATGATCTGAAACATGATTGTTTGAGCAACCGTTCTAGTTCTTCTGGCTTTGATCCCCATCACCATAGAGCTCGTCCATTGCAGGCCACTGGTTCGAGTTTGAGTTCGAATGCGAATCCATGTTCGAGTTCAAATCTGAATCAGAATCAGAATCCGAGTTCGAGTTTGAGGTCGAATCCGAATCCGAATCTGAGTTCGAGTTCAAATTCAAATTCGAATTTGAAGATGCAGTTGATTCCTTCTAAACCCGCACCAGCATCAGCTCGTGGATCTAGATTCCAAGTTCTTGAGTTGAATTTGAATGAAGAGCCAAGCCTTCCAGTTAGTGAATCTGATAAGGAAGTAAAAGAATCTCCATCGATAACCATGAAAGCTCCTTTGTTAAAACAGACCAATTTGATTCAATCTGTGCCTTTAGCTCCTTGTGTTCTTGAAGATCATCAGTTCAGGACTGAAAAAACCAGCAGCCCCACAACGCTTGCAGTTCAGAACAATGAACCACAACCACCATCATTGGCTATTAAAAGCATAGCTCCCCTGCAACCATCTTCTGCTTTAGAGGCTGGCCTCAAGTTCTATTCCACTGCAATCCAACAATCAACAATACAGAAAGCGATAAACAACACGCCATCCGAACGAATCAGTGTCGATAGTTTGCCGACTATTTACACGATTGATCCAACAATCACAAGCCTTGCAATTGAATTGTTAGAACTGTCAGCAACAACCACAAGATCAAACCAAAACGAGCATGCTATCCACATTGTGCCAACTTCAGAGGCTGTATCAATGTCTGCATCATGTTGTTCTAAGAAAATGTTGTGCTGGAATTTTCGTGCAACAGACAACGCGAAGCTAATGCGAGCATTGAAAGATCTGATTCAATTACACAAGCCATCGATTGTGCTGATCTTTGGCACCAAGATCGGTGGTACTGATGCAGATCATGTCGTTCGGGAGCTCGCTTTCGACGGTTCATATTGTAGAAAGCCTGATGGCTACAGGGGTGGAGCTTGGCTGTTGTTGTCCCAGCAAGACGTGCAAATTGAAGTCAGCTCCTACAGCCCACAGCAGGTTTCTGCATCGGTAATTCTCCCATTCTAA

Protein sequence

MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHPRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIPNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHHRARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSKPAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPCVLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTIQKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAVSMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELAFDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVILPF
Homology
BLAST of Carg25678 vs. NCBI nr
Match: KAG7030784.1 (hypothetical protein SDJN02_04821, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1148.3 bits (2969), Expect = 0.0e+00
Identity = 585/585 (100.00%), Postives = 585/585 (100.00%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
           RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK
Sbjct: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVILPF 586
           FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVILPF
Sbjct: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVILPF 585

BLAST of Carg25678 vs. NCBI nr
Match: KAG6600113.1 (hypothetical protein SDJN03_05346, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1105.1 bits (2857), Expect = 0.0e+00
Identity = 569/583 (97.60%), Postives = 572/583 (98.11%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLA RLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAARLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
            ARPLQATGSSLSSNANPCSSSNL  NQNPSSSLRSN NPNLSSSSNSNSNLKMQLIPSK
Sbjct: 241 SARPLQATGSSLSSNANPCSSSNL--NQNPSSSLRSNANPNLSSSSNSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITM  PLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMN-PLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLED+QFRTEKTSSPTTLAVQNNEPQP SLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDYQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTIT+LAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITNLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKI G DADHV RELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKISGADADHVGRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 584
           FDGSYCRKPDGY+GGAWLLLSQQDVQIEVSSYSPQQVSASVIL
Sbjct: 541 FDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 580

BLAST of Carg25678 vs. NCBI nr
Match: XP_022941630.1 (uncharacterized protein LOC111446932 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 560/583 (96.05%), Postives = 561/583 (96.23%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVT TREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
            ARPLQATGSSLSSN NPCSSSNL              NPNLSSSSNSNSNLKMQLIPSK
Sbjct: 241 SARPLQATGSSLSSNVNPCSSSNL--------------NPNLSSSSNSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITM  PLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMN-PLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLEDHQFRTEKTSSPTTLAVQNNEPQP SLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKI G DADHVVRELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKISGADADHVVRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 584
           FDGSYCRKPDGY+GGAWLLLSQQDVQIEVSSYSPQQVSASVIL
Sbjct: 541 FDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 568

BLAST of Carg25678 vs. NCBI nr
Match: XP_022941632.1 (uncharacterized protein LOC111446932 isoform X2 [Cucurbita moschata])

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 556/583 (95.37%), Postives = 558/583 (95.71%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVT TREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
            ARPLQATGSSLSSN NPCSSSNLNQN                 SS+SNSNLKMQLIPSK
Sbjct: 241 SARPLQATGSSLSSNVNPCSSSNLNQN----------------PSSSSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITM  PLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMN-PLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLEDHQFRTEKTSSPTTLAVQNNEPQP SLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKI G DADHVVRELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKISGADADHVVRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 584
           FDGSYCRKPDGY+GGAWLLLSQQDVQIEVSSYSPQQVSASVIL
Sbjct: 541 FDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 566

BLAST of Carg25678 vs. NCBI nr
Match: XP_022988533.1 (uncharacterized protein LOC111485743 isoform X1 [Cucurbita maxima] >XP_022988542.1 uncharacterized protein LOC111485743 isoform X2 [Cucurbita maxima])

HSP 1 Score: 687.2 bits (1772), Expect = 1.2e-193
Identity = 359/398 (90.20%), Postives = 364/398 (91.46%), Query Frame = 0

Query: 1   MAVQSKHFHFSRH-TGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIH 60
           MAVQSKHFH SR  TGAGNDGAAASTIGATVCNLTPS TARINQQFDQSLI WVVG KIH
Sbjct: 1   MAVQSKHFHSSRQFTGAGNDGAAASTIGATVCNLTPSLTARINQQFDQSLIAWVVGMKIH 60

Query: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWI 120
           PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSI HLCIYVFPWI
Sbjct: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSISHLCIYVFPWI 120

Query: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYAR 180
           PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIA+TIGGRLVKIDPVTETREKCMYAR
Sbjct: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAKTIGGRLVKIDPVTETREKCMYAR 180

Query: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHH 240
           ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSN S SSGFDPHH
Sbjct: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNPSCSSGFDPHH 240

Query: 241 HRARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPS 300
           HRARPLQA GS  SSN+NP SSSNL              NPNLSSS   NSNLKMQLIPS
Sbjct: 241 HRARPLQAIGS--SSNSNPSSSSNL--------------NPNLSSS--LNSNLKMQLIPS 300

Query: 301 KPAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAP 360
           KPAPASA GSRFQVLELNLNEEPSLPVSESDK VKESPSITMKAPLLKQTNLI+SVPLAP
Sbjct: 301 KPAPASACGSRFQVLELNLNEEPSLPVSESDKAVKESPSITMKAPLLKQTNLIRSVPLAP 360

Query: 361 CVLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAP 398
           CVLEDHQFRTEKTSSPTTLAV++NEPQP SLAIK IAP
Sbjct: 361 CVLEDHQFRTEKTSSPTTLAVEDNEPQPSSLAIKRIAP 380

BLAST of Carg25678 vs. ExPASy TrEMBL
Match: A0A6J1FU80 (uncharacterized protein LOC111446932 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111446932 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 560/583 (96.05%), Postives = 561/583 (96.23%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVT TREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
            ARPLQATGSSLSSN NPCSSSNL              NPNLSSSSNSNSNLKMQLIPSK
Sbjct: 241 SARPLQATGSSLSSNVNPCSSSNL--------------NPNLSSSSNSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITM  PLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMN-PLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLEDHQFRTEKTSSPTTLAVQNNEPQP SLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKI G DADHVVRELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKISGADADHVVRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 584
           FDGSYCRKPDGY+GGAWLLLSQQDVQIEVSSYSPQQVSASVIL
Sbjct: 541 FDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 568

BLAST of Carg25678 vs. ExPASy TrEMBL
Match: A0A6J1FN13 (uncharacterized protein LOC111446932 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111446932 PE=4 SV=1)

HSP 1 Score: 1080.1 bits (2792), Expect = 0.0e+00
Identity = 556/583 (95.37%), Postives = 558/583 (95.71%), Query Frame = 0

Query: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60
           MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP
Sbjct: 1   MAVQSKHFHFSRHTGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHP 60

Query: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120
           RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP
Sbjct: 61  RQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIP 120

Query: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARI 180
           NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVT TREKCMYARI
Sbjct: 121 NFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTVTREKCMYARI 180

Query: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240
           CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH
Sbjct: 181 CIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHHH 240

Query: 241 RARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPSK 300
            ARPLQATGSSLSSN NPCSSSNLNQN                 SS+SNSNLKMQLIPSK
Sbjct: 241 SARPLQATGSSLSSNVNPCSSSNLNQN----------------PSSSSNSNLKMQLIPSK 300

Query: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAPC 360
           PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITM  PLLKQTNLIQSVPLAPC
Sbjct: 301 PAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMN-PLLKQTNLIQSVPLAPC 360

Query: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420
           VLEDHQFRTEKTSSPTTLAVQNNEPQP SLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI
Sbjct: 361 VLEDHQFRTEKTSSPTTLAVQNNEPQPSSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTI 420

Query: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480
           QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV
Sbjct: 421 QKAINNTPSERISVDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTSEAV 480

Query: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELA 540
           SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKI G DADHVVRELA
Sbjct: 481 SMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKISGADADHVVRELA 540

Query: 541 FDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 584
           FDGSYCRKPDGY+GGAWLLLSQQDVQIEVSSYSPQQVSASVIL
Sbjct: 541 FDGSYCRKPDGYKGGAWLLLSQQDVQIEVSSYSPQQVSASVIL 566

BLAST of Carg25678 vs. ExPASy TrEMBL
Match: A0A6J1JDB0 (uncharacterized protein LOC111485743 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111485743 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 6.0e-194
Identity = 359/398 (90.20%), Postives = 364/398 (91.46%), Query Frame = 0

Query: 1   MAVQSKHFHFSRH-TGAGNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIH 60
           MAVQSKHFH SR  TGAGNDGAAASTIGATVCNLTPS TARINQQFDQSLI WVVG KIH
Sbjct: 1   MAVQSKHFHSSRQFTGAGNDGAAASTIGATVCNLTPSLTARINQQFDQSLIAWVVGMKIH 60

Query: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWI 120
           PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSI HLCIYVFPWI
Sbjct: 61  PRQLAVRLRRNLHLAGDLDVFELGLGFFVLKFSNALDYYEALEERPWSISHLCIYVFPWI 120

Query: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYAR 180
           PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIA+TIGGRLVKIDPVTETREKCMYAR
Sbjct: 121 PNFKPSEASIPFVDVWIRLPELSIEYYDKEVLEKIAKTIGGRLVKIDPVTETREKCMYAR 180

Query: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNRSSSSGFDPHH 240
           ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSN S SSGFDPHH
Sbjct: 181 ICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSNPSCSSGFDPHH 240

Query: 241 HRARPLQATGSSLSSNANPCSSSNLNQNQNPSSSLRSNPNPNLSSSSNSNSNLKMQLIPS 300
           HRARPLQA GS  SSN+NP SSSNL              NPNLSSS   NSNLKMQLIPS
Sbjct: 241 HRARPLQAIGS--SSNSNPSSSSNL--------------NPNLSSS--LNSNLKMQLIPS 300

Query: 301 KPAPASARGSRFQVLELNLNEEPSLPVSESDKEVKESPSITMKAPLLKQTNLIQSVPLAP 360
           KPAPASA GSRFQVLELNLNEEPSLPVSESDK VKESPSITMKAPLLKQTNLI+SVPLAP
Sbjct: 301 KPAPASACGSRFQVLELNLNEEPSLPVSESDKAVKESPSITMKAPLLKQTNLIRSVPLAP 360

Query: 361 CVLEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAP 398
           CVLEDHQFRTEKTSSPTTLAV++NEPQP SLAIK IAP
Sbjct: 361 CVLEDHQFRTEKTSSPTTLAVEDNEPQPSSLAIKRIAP 380

BLAST of Carg25678 vs. ExPASy TrEMBL
Match: A0A5A7SY10 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00450 PE=4 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 6.6e-140
Identity = 314/574 (54.70%), Postives = 366/574 (63.76%), Query Frame = 0

Query: 32  NLTPSQTARINQQFDQSLIVWVVGKKIHPRQLAVRLRRNLHLAGDLDVFELGLGFFVLKF 91
           +LTPSQTARI Q F  SLI  V G  +H R LA RLRR L L GDLDVFEL LGFFVLKF
Sbjct: 68  DLTPSQTARIAQDFHHSLIARVFGNNVHSRLLAHRLRRYLRLTGDLDVFELELGFFVLKF 127

Query: 92  SNALDYYEALEERPWSIPHLCIYVFPWIPNFKPSEASIPFVDVWIRLPELSIEYYDKEVL 151
           SN+ DY EALEE PWSI HLCI+V PW+PNFKPSEA I  VDVWIRLPEL IEYYDKE+L
Sbjct: 128 SNSSDYTEALEELPWSISHLCIHVVPWVPNFKPSEALILHVDVWIRLPELGIEYYDKEIL 187

Query: 152 EKIAETIGGRLVKIDPVTETREKCMYARICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLL 211
           EKIAE IG  LVKIDPVTE R+KCM+ARICIR+ L  PL  S QFG+  QK+ YEGLD L
Sbjct: 188 EKIAEAIGVCLVKIDPVTERRQKCMFARICIRITLCNPLIYSIQFGQTLQKVQYEGLDSL 247

Query: 212 CIVCGCVDDLKHDCL--SNRSSSSGFDPHHHRARPLQATGSSLSSNANPCSSSNLNQNQN 271
           C VCGC+D+LKH CL  +N S SSG DPH     PLQA         +P SSS L  +  
Sbjct: 248 CSVCGCIDNLKHVCLNPNNPSGSSGDDPHQQNPCPLQA--------IDPSSSSGLALDS- 307

Query: 272 PSSSLRSNPNPNLSSSSNSNSNLKMQLIPSKPAPASARGSRFQ----VLELNLNEEPSL- 331
                                  K  LI S P+  SA GS+ Q     LEL L + P L 
Sbjct: 308 -----------------------KKPLIHSLPSSESALGSKSQEKNPFLELKLKDCPKLK 367

Query: 332 --PVSESDKEV-----KESPSITMKAPLLKQTNLIQSVPLAPCVLEDHQFRTEKTSSPTT 391
              V E++K++     +ES + T + P        +SVPLA  ++ D QFR  K SSPT 
Sbjct: 368 MGKVVENEKKILPNFPEESSTTTTETP--------ESVPLAAPLVVD-QFRAAKGSSPTK 427

Query: 392 LAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTIQKAINNTPSERIS-VDS 451
           L V NN                 SSA+EAG+  +S AIQQ+  +K + NTP   I  VDS
Sbjct: 428 LPVLNNVS-------------HSSSAVEAGINSFSCAIQQTITEKKMINTPFGGIKVVDS 487

Query: 452 LPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIVPTS------EAVSMSAS---CC 511
            PT+YTIDPT  SL I+  E+  T T SNQN +AI+ V  S      E  S +AS    C
Sbjct: 488 WPTVYTIDPTTMSLGIDFSEV-PTATGSNQNRYAINFVLNSRRENDNEVDSKAASMPPLC 547

Query: 512 SKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGTDADHVVRELAFDGSYCR 571
            KKMLCWNFR  D AKL++A K LI+L +PSIVLIFG+KI   DA+ VVRELAF+GSYCR
Sbjct: 548 PKKMLCWNFRGMDIAKLIQASKYLIRLDEPSIVLIFGSKISSADAEEVVRELAFNGSYCR 586

Query: 572 KPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASV 582
           KPDGY GG W++LS QDV+IEVSSYSPQ+VSASV
Sbjct: 608 KPDGYNGGVWMILSGQDVEIEVSSYSPQKVSASV 586

BLAST of Carg25678 vs. ExPASy TrEMBL
Match: A0A5A7SSW6 (DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G00460 PE=4 SV=1)

HSP 1 Score: 471.5 bits (1212), Expect = 5.2e-129
Identity = 302/591 (51.10%), Postives = 364/591 (61.59%), Query Frame = 0

Query: 32  NLTPSQTARINQQFDQSLIVWVVGKKIHPRQLAVRLRRNLHLAGDLDVFELGLGFFVLKF 91
           NLTPSQTARINQ+F   LI  V+GK IH   L  RLR +L L GDL+V  LGLGFF L F
Sbjct: 120 NLTPSQTARINQEFRHVLIACVIGKNIHHENLTFRLRCHLPLTGDLNVSPLGLGFFALIF 179

Query: 92  SNALDYYEALEERPWSIPHLCIYVFPWIPNFKPSEASIPFVDVWIRLPELSIEYYDKEVL 151
           SN  DY EAL+ERPW IP LCI+VFPWIPNFKPS+A I FVDVW+RLPEL +E+Y++E+ 
Sbjct: 180 SNPSDYSEALKERPWLIPQLCIHVFPWIPNFKPSKAFISFVDVWVRLPELGMEHYNREMF 239

Query: 152 EKIAETIGGRLVKIDPVTETREKCMYARICIRMNLGYPLNLSFQFGKNPQKIVYEGLDLL 211
           E IA+ IG RLVKIDPVTE ++K ++ARICI + L  PL        + Q IVYEGLD L
Sbjct: 240 ENIAKAIGVRLVKIDPVTERKQKYVFARICITITLSNPLIHYIHIEGSRQNIVYEGLDSL 299

Query: 212 CIVCGCVDDLKHDCLSNRSSSSGFDPHHHRARPLQATGSSLSSNANPCSS---------- 271
           C VCGCVDDLKH CL+  +    +DPH     PLQA   S SS  +   S          
Sbjct: 300 CSVCGCVDDLKHICLNQNNPYDEYDPHQQHPCPLQAFDPSSSSGLDYLCSVCGFVYDLKH 359

Query: 272 SNLNQNQNPSSS----LRSNPNP----NLSSSSNSNSNLKMQLIPSKPAPASARGSRFQ- 331
             LN+N    SS     + NP P    + SSSS    + K  LI S P+  SA  S+ Q 
Sbjct: 360 DRLNKNSPSGSSGHDPHQQNPFPCQPIDFSSSSGLGLDSKKPLIHSLPSLESALRSKSQE 419

Query: 332 ---VLELNLNEEPSL---PVSESDKEV-----KESPSITMKAPLLKQTNLIQSVPLAPCV 391
                ELNL + P L    V E++K+      +ES + TMK          +SVPLA  +
Sbjct: 420 KDPFPELNLKDYPKLKMVKVVENEKKTLPNFPRESSTTTMKT--------TESVPLAVSL 479

Query: 392 LEDHQFRTEKTSSPTTLAVQNNEPQPPSLAIKSIAPLQPSSALEAGLKFYSTAIQQSTIQ 451
           +ED QFR  K S PT LA+ NN               + SSA+EAGL  +ST  QQ T  
Sbjct: 480 VED-QFRAAKASCPTKLALHNNGS-------------RSSSAVEAGLNLFSTVSQQLTTV 539

Query: 452 KAINNTPSERIS-VDSLPTIYTIDPTITSLAIELLELSATTTRSNQNEHAIHIV------ 511
           K + NTP   ++ VDS PT+YTIDPT  SL IE  E+  TTT SNQ ++AI+ V      
Sbjct: 540 KEMINTPFGVVNFVDSWPTVYTIDPTTMSLEIEFSEV-PTTTGSNQTQYAINFVLNPGRE 599

Query: 512 ----PTSEAVSMSASCCSKKMLCWNFRATDNAKLMRALKDLIQLHKPSIVLIFGTKIGGT 571
                 S+A SM  S CSKKMLCWNF   D A L +ALKDLI LH+PSIVLIFG+KI  +
Sbjct: 600 NENEVDSKAASM-PSLCSKKMLCWNFDGKDIALLKKALKDLIGLHEPSIVLIFGSKISSS 659

Query: 572 DADHVVRELAFDGSYCRKPDGYRGGAWLLLSQQDVQIEVSSYSPQQVSASV 582
           DAD V+REL FDG Y RKPDGY GG W++LS+QDVQ EV+S S Q+V ASV
Sbjct: 660 DADEVMRELGFDGFYSRKPDGYNGGVWMMLSRQDVQTEVNSQSSQKVFASV 686

BLAST of Carg25678 vs. TAIR 10
Match: AT2G01050.1 (zinc ion binding;nucleic acid binding )

HSP 1 Score: 100.1 bits (248), Expect = 6.1e-21
Identity = 65/212 (30.66%), Postives = 97/212 (45.75%), Query Frame = 0

Query: 17  GNDGAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHPRQLAVRLRRNLHLAGD 76
           G D     TIG  V          +N  + + +IV V+G +I    L  +LR     +G 
Sbjct: 56  GEDEEPVITIGEEVLE-------AMNGLWKKCMIVKVLGSQIPISVLNRKLRELWKPSGV 115

Query: 77  LDVFELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIPNFKPSEASIPFVDVWI 136
           + V +L   FF+++F    +Y  AL   PW +    + V  W   F P    I    VW+
Sbjct: 116 MTVMDLPRQFFMIRFELEEEYMAALTGGPWRVLGNYLLVQDWSSRFDPLRDDIVTTPVWV 175

Query: 137 RLPELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARICIRMNLGYPLNLSFQF 196
           RL  +   YY + +L +IA  + GR +K+D  T   +K  +AR+CI +NL  PL  +   
Sbjct: 176 RLSNIPYNYYHRCLLMEIARGL-GRPLKVDMNTINFDKGRFARVCIEVNLAKPLKGTVLI 235

Query: 197 GKNPQKIVYEGLDLLCIVCGCVDDLKHDCLSN 229
             +   + YEGL  +C  CG    L H C  N
Sbjct: 236 NGDRYFVAYEGLSKICSSCGIYGHLVHSCPRN 259

BLAST of Carg25678 vs. TAIR 10
Match: AT5G36228.1 (nucleic acid binding;zinc ion binding )

HSP 1 Score: 61.2 bits (147), Expect = 3.1e-09
Identity = 50/211 (23.70%), Postives = 88/211 (41.71%), Query Frame = 0

Query: 20  GAAASTIGATVCNLTPSQTARINQQFDQSLIVWVVGKKIHPRQLAVRLRRNLHLAGDLDV 79
           GA AS   + +  +   QT  + +   +    W +G ++H R L  R             
Sbjct: 30  GALASNRLSLLGRILNPQTQSVERAILELPYQWGLGTQVHGRILDDRC------------ 89

Query: 80  FELGLGFFVLKFSNALDYYEALEERPWSIPHLCIYVFPWIPNFKPSEASIPFVDVWIRLP 139
                  F ++F + +D    L   PW      I +  W  +F P+E  + F+DVW+ + 
Sbjct: 90  -------FQVRFRSEIDLLNGLRRAPWVFNEWFIALQRW-EDF-PTEDFLTFIDVWVHIR 149

Query: 140 ELSIEYYDKEVLEKIAETIGGRLVKIDPVTETREKCMYARICIRMNLGYPLNL--SFQFG 199
            + + Y  +  +E IA T+ G +V +D   ET  +  + R+ +RM+   PL      +F 
Sbjct: 150 GIPLPYVSERTVEIIASTL-GEVVAMDFNEETTSQITFIRVKVRMDFTEPLRFFRRVRFA 209

Query: 200 KNPQKIV---YEGLDLLCIVCGCVDDLKHDC 226
              + ++   YE L  +C  C  V+     C
Sbjct: 210 SRERAMIGFEYEKLQRVCTNCCRVNHQVSHC 218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030784.10.0e+00100.00hypothetical protein SDJN02_04821, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6600113.10.0e+0097.60hypothetical protein SDJN03_05346, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022941630.10.0e+0096.05uncharacterized protein LOC111446932 isoform X1 [Cucurbita moschata][more]
XP_022941632.10.0e+0095.37uncharacterized protein LOC111446932 isoform X2 [Cucurbita moschata][more]
XP_022988533.11.2e-19390.20uncharacterized protein LOC111485743 isoform X1 [Cucurbita maxima] >XP_022988542... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1FU800.0e+0096.05uncharacterized protein LOC111446932 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1FN130.0e+0095.37uncharacterized protein LOC111446932 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JDB06.0e-19490.20uncharacterized protein LOC111485743 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A5A7SY106.6e-14054.70DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A5A7SSW65.2e-12951.10DUF4283 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
Match NameE-valueIdentityDescription
AT2G01050.16.1e-2130.66zinc ion binding;nucleic acid binding [more]
AT5G36228.13.1e-0923.70nucleic acid binding;zinc ion binding [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025558Domain of unknown function DUF4283PFAMPF14111DUF4283coord: 40..182
e-value: 1.6E-25
score: 89.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 246..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 230..305
NoneNo IPR availablePANTHERPTHR31286:SF85TA11-LIKE NON-LTR RETROELEMENT PROTEIN-RELATEDcoord: 34..580
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 34..580

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25678-RACarg25678-RAmRNA