Carg25675 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25675
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCytokinin dehydrogenase
LocationCarg_Chr04: 1452904 .. 1455013 (+)
RNA-Seq ExpressionCarg25675
SyntenyCarg25675
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTACCAATCCTTTCATCTTTAACTTCAGAGACTTGTAGCCAGAGAAAATTACGATACCCACCAGTTAGTTTCCTCAAACAAACCAACATGCTTTTGATTCGAAGCTTCATGATTTTGTTCCTGAGCTGTATTACTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTTTTCGCTAAGAATGCTTTACATCAACGGCCATTTTGAATTTGACAAAGTGGAATTTGCTGCAAGAGATTTTGGTAATAGATATCAATACTACCCTGCAGCAATACTGCATCCATCGTCAGTTTCTGATATCGCCATGACAATAAAGCATATTTGGAAGATGGGACCTGGTTCAGAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTCGTGATTAACATGGAATCACTCCAGGGCCCTCAAATGCAGGTTGTTCATACCAGAAGCTTCAAATACATCGATGTTTCTGGAGGGGAGTTATGGATAAATATCCTGCAGGAGAGCTTGAAATATGGACTAACACCAAAATCATGGACAGACTACCTACATCTAACGGTTGGGGGTACTTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGACATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGTTCGTTGCCATATACATCATGCTATACATATACAAAAGTAATGTGATAGGAAATCTATGCAAGGTAAATGGCGAACGCCATTTCCTGGATGCTATATTGTTATGGTAACTGACAAATGGAGTGAAACTGACTTCAGGAAAAGGGGACGTGGTAAACTGCTCAGAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGGTTAGGCCAATTTGGAATTATCACACGGGCAAAAATTCTACTTGAACCAGCGCCAACTATGGTAAGATTAGTGTTCTAAAGGAACTTTGAATCTAGTCTAAAGTAACTATGAAGGGCTGTTCTAACCCAATTCTTCTATCCTTGACAGGTAAAATGGATAAGAGTATTGTACTTTGACTTCACAACATTTACCAGAGAGCAGGAGCTGTTAATATCTGGAGAAAATACATTCGATTATATTGAAGGATTCGTGATAATAAACAGAACTGGTCTTCTAAATAATTGGAGATCATCATTCGATCCACAAGACCCTGTTCAAGCAAGCCAGTTCAAGTCTGAAGGAAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACAAGGACAACAACAATGTCGATCAGGTGAAGTATCCCTACTAAATAAATCTAATGGAAAATGTGAAAAATGTGAAAAATGTGAAAAATGTAAAATGTGAAAAATGTGAAAATAAATGGCAGCTACATGAACATGCTTTTACCATGCAGGAAGTCAAGCGGTTGTTGTCTCGGTTGAGTTATATCCCATCAACGCTTTTCATATCAGAAGTTACATACGTGGAATTCCTGGACAGAGTTCAAGTATCAGCCGTGAAATTACAGTCAAAAGGGTTGTGGGAAGTCCCACACCCATGGGTCTGTAATTGTATACTTATCCCCTAAAGCTAAGATAATAATACACAGGAATTTGCGATGGGTTTTTTTGGCAATATCCTGAAGTGAACAAGCATAATCGGCCGCAGTCCTAATTCTACCGTGTTTTTAACAAATCAGAAGTAATTAATGATGCGTAACAATTGTTAAACTGTGCTTTGGCCAATATTTGCTATTATTTATTATATAGTTATTATTCATTTGAATATACTGATTCTATTAATTGTATACTGAGTTTTTCCAGGTTGGAGACTAATTGGTAGACACAATCCGTTAGTTTATTCCCAGAGGAGAGAGTTATCTTTGCGTACCTGGTTGCCATTTTCCTGACCTTTCCTGCAGTCCGAGTTTCTAGAGGAATACGACATGACTTAGCCAATACATTTAAGCTTAACAGAACAACTATTCGAGAATATCTGGAATTACTGTTATAAACACCAATTTAGGAGCAAAACTATAAGTATTTTGTCCACATTACACTACAAGGAAAGAATGGCAGGCCCATTTTGGGCCAATGTGGGAAACATATGTAG

mRNA sequence

ATGTTACCAATCCTTTCATCTTTAACTTCAGAGACTTGTAGCCAGAGAAAATTACGATACCCACCAGTTAGTTTCCTCAAACAAACCAACATGCTTTTGATTCGAAGCTTCATGATTTTGTTCCTGAGCTGTATTACTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTTTTCGCTAAGAATGCTTTACATCAACGGCCATTTTGAATTTGACAAAGTGGAATTTGCTGCAAGAGATTTTGGTAATAGATATCAATACTACCCTGCAGCAATACTGCATCCATCGTCAGTTTCTGATATCGCCATGACAATAAAGCATATTTGGAAGATGGGACCTGGTTCAGAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTCGTGATTAACATGGAATCACTCCAGGGCCCTCAAATGCAGGTTGTTCATACCAGAAGCTTCAAATACATCGATGTTTCTGGAGGGGAGTTATGGATAAATATCCTGCAGGAGAGCTTGAAATATGGACTAACACCAAAATCATGGACAGACTACCTACATCTAACGGTTGGGGGTACTTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGACATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGAAAAGGGGACGTGGTAAACTGCTCAGAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGGTTAGGCCAATTTGGAATTATCACACGGGCAAAAATTCTACTTGAACCAGCGCCAACTATGGTAAAATGGATAAGAGTATTGTACTTTGACTTCACAACATTTACCAGAGAGCAGGAGCTGTTAATATCTGGAGAAAATACATTCGATTATATTGAAGGATTCGTGATAATAAACAGAACTGGTCTTCTAAATAATTGGAGATCATCATTCGATCCACAAGACCCTGTTCAAGCAAGCCAGTTCAAGTCTGAAGGAAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACAAGGACAACAACAATGTCGATCAGGAAGTCAAGCGGTTGTTGTCTCGGTTGAGTTATATCCCATCAACGCTTTTCATATCAGAAGTTACATACGTGGAATTCCTGGACAGAGTTCAAGTATCAGCCGTGAAATTACAGTCAAAAGGGTTGTGGGAAGTCCCACACCCATGGGAGCAAAACTATAAGTATTTTGTCCACATTACACTACAAGGAAAGAATGGCAGGCCCATTTTGGGCCAATGTGGGAAACATATGTAG

Coding sequence (CDS)

ATGTTACCAATCCTTTCATCTTTAACTTCAGAGACTTGTAGCCAGAGAAAATTACGATACCCACCAGTTAGTTTCCTCAAACAAACCAACATGCTTTTGATTCGAAGCTTCATGATTTTGTTCCTGAGCTGTATTACTGTTAGGATGAATCTATGCTTCACAGGCATCCCTTTTTCGCTAAGAATGCTTTACATCAACGGCCATTTTGAATTTGACAAAGTGGAATTTGCTGCAAGAGATTTTGGTAATAGATATCAATACTACCCTGCAGCAATACTGCATCCATCGTCAGTTTCTGATATCGCCATGACAATAAAGCATATTTGGAAGATGGGACCTGGTTCAGAGCTCACAGTTGCAGCTAGAGGCCATGGCCACTCACTTCATGGCCAAGCACAGGCCCACCAAGGAGTCGTGATTAACATGGAATCACTCCAGGGCCCTCAAATGCAGGTTGTTCATACCAGAAGCTTCAAATACATCGATGTTTCTGGAGGGGAGTTATGGATAAATATCCTGCAGGAGAGCTTGAAATATGGACTAACACCAAAATCATGGACAGACTACCTACATCTAACGGTTGGGGGTACTTTATCCAATGCAGGGATCAGTGGACAGGCATTTCGACATGGCCCTCAGATCAGCAACGTTCATCAGCTGGAGGTTGTAACAGGAAAAGGGGACGTGGTAAACTGCTCAGAGGAACAGAACAGTGACCTCTTTTACAGTGTTCTTGGAGGGTTAGGCCAATTTGGAATTATCACACGGGCAAAAATTCTACTTGAACCAGCGCCAACTATGGTAAAATGGATAAGAGTATTGTACTTTGACTTCACAACATTTACCAGAGAGCAGGAGCTGTTAATATCTGGAGAAAATACATTCGATTATATTGAAGGATTCGTGATAATAAACAGAACTGGTCTTCTAAATAATTGGAGATCATCATTCGATCCACAAGACCCTGTTCAAGCAAGCCAGTTCAAGTCTGAAGGAAAAATTCTTTACTGCCTAGAATTGGCAAAGTACTTTCACCACAACAAGGACAACAACAATGTCGATCAGGAAGTCAAGCGGTTGTTGTCTCGGTTGAGTTATATCCCATCAACGCTTTTCATATCAGAAGTTACATACGTGGAATTCCTGGACAGAGTTCAAGTATCAGCCGTGAAATTACAGTCAAAAGGGTTGTGGGAAGTCCCACACCCATGGGAGCAAAACTATAAGTATTTTGTCCACATTACACTACAAGGAAAGAATGGCAGGCCCATTTTGGGCCAATGTGGGAAACATATGTAG

Protein sequence

MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEGFVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWEQNYKYFVHITLQGKNGRPILGQCGKHM
Homology
BLAST of Carg25675 vs. NCBI nr
Match: KAG7030781.1 (Cytokinin dehydrogenase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 884.8 bits (2285), Expect = 3.0e-253
Identity = 432/432 (100.00%), Postives = 432/432 (100.00%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWEQNYKYFVHITLQGKN 420
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWEQNYKYFVHITLQGKN
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPWEQNYKYFVHITLQGKN 420

Query: 421 GRPILGQCGKHM 433
           GRPILGQCGKHM
Sbjct: 421 GRPILGQCGKHM 432

BLAST of Carg25675 vs. NCBI nr
Match: XP_022941633.1 (cytokinin dehydrogenase 6-like [Cucurbita moschata])

HSP 1 Score: 815.8 bits (2106), Expect = 1.7e-232
Identity = 401/404 (99.26%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVH RSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHNRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 404

BLAST of Carg25675 vs. NCBI nr
Match: XP_023525504.1 (cytokinin dehydrogenase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 809.7 bits (2090), Expect = 1.2e-230
Identity = 398/404 (98.51%), Postives = 400/404 (99.01%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNML IRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHT+SF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTKSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLIS ENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISEENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 404

BLAST of Carg25675 vs. NCBI nr
Match: XP_022988501.1 (cytokinin dehydrogenase 6-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 807.7 bits (2085), Expect = 4.7e-230
Identity = 396/404 (98.02%), Postives = 400/404 (99.01%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNML IRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEF+KVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFEKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAH+GVVINMESLQGPQMQVVHTRSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHRGVVINMESLQGPQMQVVHTRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVD E+KRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDLEIKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 404

BLAST of Carg25675 vs. NCBI nr
Match: XP_022988509.1 (cytokinin dehydrogenase 6-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 800.0 bits (2065), Expect = 9.8e-228
Identity = 395/410 (96.34%), Postives = 400/410 (97.56%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNML IRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEF+KVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFEKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAH+GVVINMESLQGPQMQVVHTRSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHRGVVINMESLQGPQMQVVHTRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVD------ 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNN+       
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNLHEHAFTM 360

Query: 361 QEVKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           QE+KRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 QEIKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 410

BLAST of Carg25675 vs. ExPASy Swiss-Prot
Match: Q9LY71 (Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2)

HSP 1 Score: 522.7 bits (1345), Expect = 3.9e-147
Identity = 261/390 (66.92%), Postives = 307/390 (78.72%), Query Frame = 0

Query: 18  LRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFA 77
           + Y   S L++  ML++RSF IL LSCI  ++  CF+    SL+ L + GH EF+ V  A
Sbjct: 1   MSYLHASLLRKRTMLIVRSFTILLLSCIAFKLACCFSSSISSLKALPLVGHLEFEHVHHA 60

Query: 78  ARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQAHQG 137
           ++DFGNRYQ  P A+LHP SVSDIA TI+HIW MG  S+LTVAARG GHSL GQAQ   G
Sbjct: 61  SKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTRHG 120

Query: 138 VVINMESLQGPQMQVVHTRS-FKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGG 197
           +VI+MESL   ++QV    S   Y+DVSGGELWINIL E+LKYGL PKSWTDYLHLTVGG
Sbjct: 121 IVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTVGG 180

Query: 198 TLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITR 257
           TLSNAGISGQAFRHGPQISNVHQLE+VTGKG+++NC++ QNSDLF  VLGGLGQFGIITR
Sbjct: 181 TLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGIITR 240

Query: 258 AKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGE-NTFDYIEGFVIINRTGLLNNWRS 317
           A+I LEPAPTMVKWIRVLY DF  F ++QE LIS + + FDYIEGFVIINRTGLLN+WR 
Sbjct: 241 ARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSWRL 300

Query: 318 SFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNV-DQEVKRLLSRLSYIPSTLFIS 377
           SF  ++P++ASQFK +G+ LYCLELAKY    +DN +V +QEVK  LS LSY+ STLF +
Sbjct: 301 SFTAEEPLEASQFKFDGRTLYCLELAKYL--KQDNKDVINQEVKETLSELSYVTSTLFTT 360

Query: 378 EVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           EV Y  FLDRV VS VKL+SKG WEVPHPW
Sbjct: 361 EVAYEAFLDRVHVSEVKLRSKGQWEVPHPW 388

BLAST of Carg25675 vs. ExPASy Swiss-Prot
Match: O22213 (Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 2.6e-143
Identity = 257/395 (65.06%), Postives = 306/395 (77.47%), Query Frame = 0

Query: 25  FLKQTNMLLIRSFMILFLSCITVRMNLCF---------------TGIPFSLRMLYINGHF 84
           F +Q N   +  FMIL LSCI  R NLC                + I  SL  L + G+ 
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLEGYI 68

Query: 85  EFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLH 144
            FD V   A+DFGNRYQ  P AILHP SV DI+  +KHI  +G  S LTVAARGHGHSL 
Sbjct: 69  SFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQ 128

Query: 145 GQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDY 204
           GQA AHQGVVI MESL+ P +++   +   Y+DVSGGE+WINIL+E+LKYGL+PKSWTDY
Sbjct: 129 GQALAHQGVVIKMESLRSPDIRIYKGKQ-PYVDVSGGEIWINILRETLKYGLSPKSWTDY 188

Query: 205 LHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLG 264
           LHLTVGGTLSNAGISGQAF+HGPQI+NV+QLE+VTGKG+VV CSE++NS+LF+SVLGGLG
Sbjct: 189 LHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLG 248

Query: 265 QFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEGFVIINRTGL 324
           QFGIITRA+I LEPAP MVKWIRVLY DF+ F+R+QE LIS E TFDY+EGFVIINRT L
Sbjct: 249 QFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDL 308

Query: 325 LNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPS 384
           LNNWRSSF P D  QAS+FKS+GK LYCLE+ KYF + ++ +++DQE  +LLS L+YIPS
Sbjct: 309 LNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYF-NPEEASSMDQETGKLLSELNYIPS 368

Query: 385 TLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           TLF SEV Y+EFLDRV ++  KL++KGLWEVPHPW
Sbjct: 369 TLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPW 401

BLAST of Carg25675 vs. ExPASy Swiss-Prot
Match: Q75K78 (Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.0e-139
Identity = 239/372 (64.25%), Postives = 303/372 (81.45%), Query Frame = 0

Query: 34  IRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFAARDFGNRYQYYPAAIL 93
           ++  ++L L  +T  M++    +P SL  L ++GHF F  V  AA+DFGN   + P A+L
Sbjct: 9   LKLLLLLALGGVTT-MHVPKQDVPSSLEELTLDGHFSFHDVSAAAQDFGNLSSFPPVAVL 68

Query: 94  HPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQAHQGVVINMESLQGPQMQVV 153
           HP SV+DIA TI+H++ MG  S LTVAARGHGHSL+GQ+QA +G++I+MESLQ   M+ V
Sbjct: 69  HPGSVADIATTIRHVFLMGEHSTLTVAARGHGHSLYGQSQAAEGIIISMESLQSNTMR-V 128

Query: 154 HTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQ 213
           +     Y+D SGGELWIN+L E+LKYGL PKSWTDYLHLTVGGTLSNAG+SGQ FRHGPQ
Sbjct: 129 NPGVSPYVDASGGELWINVLHETLKYGLAPKSWTDYLHLTVGGTLSNAGVSGQTFRHGPQ 188

Query: 214 ISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRV 273
           ISNV++LE+VTG+GDV+ CS EQNSDLF++ LGGLGQFG+ITRA+I LEPAP MV+W+RV
Sbjct: 189 ISNVNELEIVTGRGDVITCSPEQNSDLFHAALGGLGQFGVITRARIPLEPAPKMVRWLRV 248

Query: 274 LYFDFTTFTREQELLISGENTFDYIEGFVIINRTGLLNNWRSSFDPQDPVQASQFKSEGK 333
           LY DFT+FT +QE+LIS E TFDYIEGFVIINRTG+LNNWRSSF+PQDPV++SQF+S+GK
Sbjct: 249 LYLDFTSFTEDQEMLISAEKTFDYIEGFVIINRTGILNNWRSSFNPQDPVRSSQFESDGK 308

Query: 334 ILYCLELAKYFHHNKDNNNV-DQEVKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKL 393
           +L+CLE+ K F  N D  +V +QEV  LLS+L Y+PS+LF ++VTY+EFLDRV  S +KL
Sbjct: 309 VLFCLEMTKNF--NPDEADVMEQEVNTLLSQLRYMPSSLFHTDVTYIEFLDRVHSSEMKL 368

Query: 394 QSKGLWEVPHPW 405
           ++KG+WEVPHPW
Sbjct: 369 RAKGMWEVPHPW 376

BLAST of Carg25675 vs. ExPASy Swiss-Prot
Match: Q5JLP4 (Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 SV=1)

HSP 1 Score: 488.4 bits (1256), Expect = 8.2e-137
Identity = 232/348 (66.67%), Postives = 283/348 (81.32%), Query Frame = 0

Query: 59  SLRMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELT 118
           SL  L ++GHF FD    AARDFGNR    PAA+LHP SVSD+A T++ ++++G  S LT
Sbjct: 37  SLGALRLDGHFSFDDAHAAARDFGNRCSLLPAAVLHPGSVSDVAATVRRVFQLGRSSPLT 96

Query: 119 VAARGHGHSLHGQAQAHQGVVINMESLQGPQMQV--VHTRSFKYIDVSGGELWINILQES 178
           VAARGHGHSL GQ+QA  G+V+ MESL     +   VH  +  ++D  GGELWIN+L E+
Sbjct: 97  VAARGHGHSLLGQSQAAGGIVVKMESLAAAAARAVRVHGGASPHVDAPGGELWINVLHET 156

Query: 179 LKYGLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQ 238
           LK+GL P+SWTDYLHLTVGGTLSNAG+SGQAFRHGPQ+SNV+QLE+VTG+G+VV CS E 
Sbjct: 157 LKHGLAPRSWTDYLHLTVGGTLSNAGVSGQAFRHGPQVSNVNQLEIVTGRGEVVTCSHEV 216

Query: 239 NSDLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFD 298
           NSDLFY+ LGGLGQFGIITRA+I LEPAP MV+WIRVLY DF TFT +QE LI+ E TFD
Sbjct: 217 NSDLFYAALGGLGQFGIITRARIALEPAPKMVRWIRVLYSDFETFTEDQEKLIASEKTFD 276

Query: 299 YIEGFVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQE 358
           YIEGFVIINRTG+LNNWR+SF PQDPVQASQF+S+G++LYCLEL   F+H+ + + ++QE
Sbjct: 277 YIEGFVIINRTGILNNWRTSFKPQDPVQASQFQSDGRVLYCLELTMNFNHD-EADIMEQE 336

Query: 359 VKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           V  LLSRL YI STLF ++VTY+EFLDRV  S +KL+++GLWEVPHPW
Sbjct: 337 VGALLSRLRYISSTLFYTDVTYLEFLDRVHTSELKLRAQGLWEVPHPW 383

BLAST of Carg25675 vs. ExPASy Swiss-Prot
Match: Q67YU0 (Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1)

HSP 1 Score: 371.3 bits (952), Expect = 1.5e-101
Identity = 199/386 (51.55%), Postives = 274/386 (70.98%), Query Frame = 0

Query: 27  KQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFA--ARDFGN- 86
           + T+  L+ +F I  L  I V +N+  + +   +  + ++GHF     + A  + DFG  
Sbjct: 4   EMTSSFLLLTFAICKL-IIAVGLNVGPSEL-LRIGAIDVDGHFTVHPSDLASVSSDFGML 63

Query: 87  RYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQA-HQGVVINM 146
           +    P A+LHPSS  D+A  ++  +  G  +   V+ARGHGHS++GQA A   GVV+ M
Sbjct: 64  KSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPVSARGHGHSINGQAAAGRNGVVVEM 123

Query: 147 -ESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGGTLSNA 206
              + G    +V      Y+DV GGELW+++L+++L++GL PKSWTDYL+LTVGGTLSNA
Sbjct: 124 NHGVTGTPKPLVRPDEM-YVDVWGGELWVDVLKKTLEHGLAPKSWTDYLYLTVGGTLSNA 183

Query: 207 GISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITRAKILL 266
           GISGQAF HGPQISNV +L+VVTGKG+V+ CSEE+N+ LF+ VLGGLGQFGIITRA+I L
Sbjct: 184 GISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGVLGGLGQFGIITRARISL 243

Query: 267 EPAPTMVKWIRVLYFDFTTFTREQELLIS--GENTFDYIEGFVIINRTGLLNNWRSS-FD 326
           EPAP  V+WIRVLY  F  FT +QE LIS  G+  FDY+EGFVI++  GL+NNWRSS F 
Sbjct: 244 EPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFDYVEGFVIVDE-GLVNNWRSSFFS 303

Query: 327 PQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPSTLFISEVTY 386
           P++PV+ S   S G +LYCLE+ K + H+ D+  VDQEV+ L+ +L++IP+++F +++ Y
Sbjct: 304 PRNPVKISSVSSNGSVLYCLEITKNY-HDSDSEIVDQEVEILMKKLNFIPTSVFTTDLQY 363

Query: 387 VEFLDRVQVSAVKLQSKGLWEVPHPW 405
           V+FLDRV  + +KL+SK LWEVPHPW
Sbjct: 364 VDFLDRVHKAELKLRSKNLWEVPHPW 382

BLAST of Carg25675 vs. ExPASy TrEMBL
Match: A0A6J1FSN0 (Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111446933 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 8.3e-233
Identity = 401/404 (99.26%), Postives = 401/404 (99.26%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVH RSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHNRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 404

BLAST of Carg25675 vs. ExPASy TrEMBL
Match: A0A6J1JHC8 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1)

HSP 1 Score: 807.7 bits (2085), Expect = 2.3e-230
Identity = 396/404 (98.02%), Postives = 400/404 (99.01%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNML IRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEF+KVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFEKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAH+GVVINMESLQGPQMQVVHTRSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHRGVVINMESLQGPQMQVVHTRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVD E+KRL
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDLEIKRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 404

BLAST of Carg25675 vs. ExPASy TrEMBL
Match: A0A6J1JLT1 (Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.7e-228
Identity = 395/410 (96.34%), Postives = 400/410 (97.56%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLPILSSLTSETCSQRKLRYPPVSFLKQTNML IRSFMILFLSCI VRMNLCFTGIPFSL
Sbjct: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLSIRSFMILFLSCIAVRMNLCFTGIPFSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           RMLYINGHFEF+KVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA
Sbjct: 61  RMLYINGHFEFEKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAH+GVVINMESLQGPQMQVVHTRSF YIDVSGGELWINILQESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHRGVVINMESLQGPQMQVVHTRSFTYIDVSGGELWINILQESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVD------ 360
           FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNN+       
Sbjct: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNLHEHAFTM 360

Query: 361 QEVKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           QE+KRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 QEIKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 410

BLAST of Carg25675 vs. ExPASy TrEMBL
Match: A0A6J1CFB1 (Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010228 PE=3 SV=1)

HSP 1 Score: 744.6 bits (1921), Expect = 2.4e-211
Identity = 368/404 (91.09%), Postives = 383/404 (94.80%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLP+LSSLTSETC+QRKLRYPPVSFL+Q NML IRSFMILFLSCI +RMNLCF GIP SL
Sbjct: 1   MLPLLSSLTSETCTQRKLRYPPVSFLRQNNMLSIRSFMILFLSCIALRMNLCFNGIPSSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           +ML INGHFEFDKV++AARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGP SELTVA
Sbjct: 61  KMLSINGHFEFDKVQYAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPRSELTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ VHTR F YIDV GGELWINIL ESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQ-VHTRDFTYIDVPGGELWINILHESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFG+ITRAKILLEPAP  VKWIRVLY DFTTF+REQELLIS ENTF+YIEG
Sbjct: 241 FYSVLGGLGQFGVITRAKILLEPAPAKVKWIRVLYLDFTTFSREQELLISAENTFNYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSF+PQDPVQASQFKS+GKILYCLELAKYF+HN + N VDQEVK L
Sbjct: 301 FVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFNHN-EGNIVDQEVKSL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LSRLSYIPSTLFISEVTYVEFLDRV VSAVKLQSKGLWEVPHPW
Sbjct: 361 LSRLSYIPSTLFISEVTYVEFLDRVHVSAVKLQSKGLWEVPHPW 402

BLAST of Carg25675 vs. ExPASy TrEMBL
Match: A0A1S3BD71 (Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488756 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 8.4e-209
Identity = 362/404 (89.60%), Postives = 384/404 (95.05%), Query Frame = 0

Query: 1   MLPILSSLTSETCSQRKLRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSL 60
           MLP+LSSLTSETC+QRK RYPPVSFL+Q NML IRSF+I FLS I  RMNLCFTGIP SL
Sbjct: 1   MLPLLSSLTSETCTQRKTRYPPVSFLRQNNMLSIRSFVIFFLSFIAFRMNLCFTGIPSSL 60

Query: 61  RMLYINGHFEFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVA 120
           +ML I+GHFEFD+V+FAARDFGNRYQ++PAAILHPSSVSDIAMTIKHIWKMGP S+LTVA
Sbjct: 61  KMLSIDGHFEFDQVQFAARDFGNRYQFFPAAILHPSSVSDIAMTIKHIWKMGPRSQLTVA 120

Query: 121 ARGHGHSLHGQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYG 180
           ARGHGHSLHGQAQAHQGVVINM+SLQGPQMQ VHTR+F YIDVSGGELWINIL ESLKYG
Sbjct: 121 ARGHGHSLHGQAQAHQGVVINMKSLQGPQMQ-VHTRNFTYIDVSGGELWINILHESLKYG 180

Query: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDL 240
           LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLE+VTGKGDVVNCS+EQNSDL
Sbjct: 181 LTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEIVTGKGDVVNCSKEQNSDL 240

Query: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEG 300
           FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLY DFTTF+REQELLIS +NTFDYIEG
Sbjct: 241 FYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYLDFTTFSREQELLISAQNTFDYIEG 300

Query: 301 FVIINRTGLLNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRL 360
           FVIINRTGLLNNWRSSF+PQDPVQASQFKS+GKILYCLELAKYFHH  + N +DQEV RL
Sbjct: 301 FVIINRTGLLNNWRSSFNPQDPVQASQFKSDGKILYCLELAKYFHHT-EGNIIDQEVMRL 360

Query: 361 LSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           LS+LSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW
Sbjct: 361 LSQLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 402

BLAST of Carg25675 vs. TAIR 10
Match: AT3G63440.1 (cytokinin oxidase/dehydrogenase 6 )

HSP 1 Score: 522.7 bits (1345), Expect = 2.8e-148
Identity = 261/390 (66.92%), Postives = 307/390 (78.72%), Query Frame = 0

Query: 18  LRYPPVSFLKQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFA 77
           + Y   S L++  ML++RSF IL LSCI  ++  CF+    SL+ L + GH EF+ V  A
Sbjct: 1   MSYLHASLLRKRTMLIVRSFTILLLSCIAFKLACCFSSSISSLKALPLVGHLEFEHVHHA 60

Query: 78  ARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQAHQG 137
           ++DFGNRYQ  P A+LHP SVSDIA TI+HIW MG  S+LTVAARG GHSL GQAQ   G
Sbjct: 61  SKDFGNRYQLIPLAVLHPKSVSDIASTIRHIWMMGTHSQLTVAARGRGHSLQGQAQTRHG 120

Query: 138 VVINMESLQGPQMQVVHTRS-FKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGG 197
           +VI+MESL   ++QV    S   Y+DVSGGELWINIL E+LKYGL PKSWTDYLHLTVGG
Sbjct: 121 IVIHMESLHPQKLQVYSVDSPAPYVDVSGGELWINILHETLKYGLAPKSWTDYLHLTVGG 180

Query: 198 TLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITR 257
           TLSNAGISGQAFRHGPQISNVHQLE+VTGKG+++NC++ QNSDLF  VLGGLGQFGIITR
Sbjct: 181 TLSNAGISGQAFRHGPQISNVHQLEIVTGKGEILNCTKRQNSDLFNGVLGGLGQFGIITR 240

Query: 258 AKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGE-NTFDYIEGFVIINRTGLLNNWRS 317
           A+I LEPAPTMVKWIRVLY DF  F ++QE LIS + + FDYIEGFVIINRTGLLN+WR 
Sbjct: 241 ARIALEPAPTMVKWIRVLYLDFAAFAKDQEQLISAQGHKFDYIEGFVIINRTGLLNSWRL 300

Query: 318 SFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNV-DQEVKRLLSRLSYIPSTLFIS 377
           SF  ++P++ASQFK +G+ LYCLELAKY    +DN +V +QEVK  LS LSY+ STLF +
Sbjct: 301 SFTAEEPLEASQFKFDGRTLYCLELAKYL--KQDNKDVINQEVKETLSELSYVTSTLFTT 360

Query: 378 EVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           EV Y  FLDRV VS VKL+SKG WEVPHPW
Sbjct: 361 EVAYEAFLDRVHVSEVKLRSKGQWEVPHPW 388

BLAST of Carg25675 vs. TAIR 10
Match: AT2G41510.1 (cytokinin oxidase/dehydrogenase 1 )

HSP 1 Score: 510.0 bits (1312), Expect = 1.9e-144
Identity = 257/395 (65.06%), Postives = 306/395 (77.47%), Query Frame = 0

Query: 25  FLKQTNMLLIRSFMILFLSCITVRMNLCF---------------TGIPFSLRMLYINGHF 84
           F +Q N   +  FMIL LSCI  R NLC                + I  SL  L + G+ 
Sbjct: 9   FHRQNNKTFLGIFMILVLSCIPGRTNLCSNHSVSTPKELPSSNPSDIRSSLVSLDLEGYI 68

Query: 85  EFDKVEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLH 144
            FD V   A+DFGNRYQ  P AILHP SV DI+  +KHI  +G  S LTVAARGHGHSL 
Sbjct: 69  SFDDVHNVAKDFGNRYQLPPLAILHPRSVFDISSMMKHIVHLGSTSNLTVAARGHGHSLQ 128

Query: 145 GQAQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDY 204
           GQA AHQGVVI MESL+ P +++   +   Y+DVSGGE+WINIL+E+LKYGL+PKSWTDY
Sbjct: 129 GQALAHQGVVIKMESLRSPDIRIYKGKQ-PYVDVSGGEIWINILRETLKYGLSPKSWTDY 188

Query: 205 LHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLG 264
           LHLTVGGTLSNAGISGQAF+HGPQI+NV+QLE+VTGKG+VV CSE++NS+LF+SVLGGLG
Sbjct: 189 LHLTVGGTLSNAGISGQAFKHGPQINNVYQLEIVTGKGEVVTCSEKRNSELFFSVLGGLG 248

Query: 265 QFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEGFVIINRTGL 324
           QFGIITRA+I LEPAP MVKWIRVLY DF+ F+R+QE LIS E TFDY+EGFVIINRT L
Sbjct: 249 QFGIITRARISLEPAPHMVKWIRVLYSDFSAFSRDQEYLISKEKTFDYVEGFVIINRTDL 308

Query: 325 LNNWRSSFDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPS 384
           LNNWRSSF P D  QAS+FKS+GK LYCLE+ KYF + ++ +++DQE  +LLS L+YIPS
Sbjct: 309 LNNWRSSFSPNDSTQASRFKSDGKTLYCLEVVKYF-NPEEASSMDQETGKLLSELNYIPS 368

Query: 385 TLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           TLF SEV Y+EFLDRV ++  KL++KGLWEVPHPW
Sbjct: 369 TLFSSEVPYIEFLDRVHIAERKLRAKGLWEVPHPW 401

BLAST of Carg25675 vs. TAIR 10
Match: AT1G75450.1 (cytokinin oxidase 5 )

HSP 1 Score: 371.3 bits (952), Expect = 1.0e-102
Identity = 199/386 (51.55%), Postives = 274/386 (70.98%), Query Frame = 0

Query: 27  KQTNMLLIRSFMILFLSCITVRMNLCFTGIPFSLRMLYINGHFEFDKVEFA--ARDFGN- 86
           + T+  L+ +F I  L  I V +N+  + +   +  + ++GHF     + A  + DFG  
Sbjct: 4   EMTSSFLLLTFAICKL-IIAVGLNVGPSEL-LRIGAIDVDGHFTVHPSDLASVSSDFGML 63

Query: 87  RYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAARGHGHSLHGQAQA-HQGVVINM 146
           +    P A+LHPSS  D+A  ++  +  G  +   V+ARGHGHS++GQA A   GVV+ M
Sbjct: 64  KSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPVSARGHGHSINGQAAAGRNGVVVEM 123

Query: 147 -ESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDYLHLTVGGTLSNA 206
              + G    +V      Y+DV GGELW+++L+++L++GL PKSWTDYL+LTVGGTLSNA
Sbjct: 124 NHGVTGTPKPLVRPDEM-YVDVWGGELWVDVLKKTLEHGLAPKSWTDYLYLTVGGTLSNA 183

Query: 207 GISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQFGIITRAKILL 266
           GISGQAF HGPQISNV +L+VVTGKG+V+ CSEE+N+ LF+ VLGGLGQFGIITRA+I L
Sbjct: 184 GISGQAFHHGPQISNVLELDVVTGKGEVMRCSEEENTRLFHGVLGGLGQFGIITRARISL 243

Query: 267 EPAPTMVKWIRVLYFDFTTFTREQELLIS--GENTFDYIEGFVIINRTGLLNNWRSS-FD 326
           EPAP  V+WIRVLY  F  FT +QE LIS  G+  FDY+EGFVI++  GL+NNWRSS F 
Sbjct: 244 EPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFDYVEGFVIVDE-GLVNNWRSSFFS 303

Query: 327 PQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPSTLFISEVTY 386
           P++PV+ S   S G +LYCLE+ K + H+ D+  VDQEV+ L+ +L++IP+++F +++ Y
Sbjct: 304 PRNPVKISSVSSNGSVLYCLEITKNY-HDSDSEIVDQEVEILMKKLNFIPTSVFTTDLQY 363

Query: 387 VEFLDRVQVSAVKLQSKGLWEVPHPW 405
           V+FLDRV  + +KL+SK LWEVPHPW
Sbjct: 364 VDFLDRVHKAELKLRSKNLWEVPHPW 382

BLAST of Carg25675 vs. TAIR 10
Match: AT1G75450.2 (cytokinin oxidase 5 )

HSP 1 Score: 370.9 bits (951), Expect = 1.4e-102
Identity = 191/349 (54.73%), Postives = 256/349 (73.35%), Query Frame = 0

Query: 65  INGHFEFDKVEFA--ARDFGN-RYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELTVAA 124
           ++GHF     + A  + DFG  +    P A+LHPSS  D+A  ++  +  G  +   V+A
Sbjct: 36  VDGHFTVHPSDLASVSSDFGMLKSPEEPLAVLHPSSAEDVARLVRTAY--GSATAFPVSA 95

Query: 125 RGHGHSLHGQAQA-HQGVVINM-ESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKY 184
           RGHGHS++GQA A   GVV+ M   + G    +V      Y+DV GGELW+++L+++L++
Sbjct: 96  RGHGHSINGQAAAGRNGVVVEMNHGVTGTPKPLVRPDEM-YVDVWGGELWVDVLKKTLEH 155

Query: 185 GLTPKSWTDYLHLTVGGTLSNAGISGQAFRHGPQISNVHQLEVVT-GKGDVVNCSEEQNS 244
           GL PKSWTDYL+LTVGGTLSNAGISGQAF HGPQISNV +L+VVT GKG+V+ CSEE+N+
Sbjct: 156 GLAPKSWTDYLYLTVGGTLSNAGISGQAFHHGPQISNVLELDVVTVGKGEVMRCSEEENT 215

Query: 245 DLFYSVLGGLGQFGIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLIS--GENTFD 304
            LF+ VLGGLGQFGIITRA+I LEPAP  V+WIRVLY  F  FT +QE LIS  G+  FD
Sbjct: 216 RLFHGVLGGLGQFGIITRARISLEPAPQRVRWIRVLYSSFKVFTEDQEYLISMHGQLKFD 275

Query: 305 YIEGFVIINRTGLLNNWRSS-FDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQ 364
           Y+EGFVI++  GL+NNWRSS F P++PV+ S   S G +LYCLE+ K + H+ D+  VDQ
Sbjct: 276 YVEGFVIVDE-GLVNNWRSSFFSPRNPVKISSVSSNGSVLYCLEITKNY-HDSDSEIVDQ 335

Query: 365 EVKRLLSRLSYIPSTLFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           EV+ L+ +L++IP+++F +++ YV+FLDRV  + +KL+SK LWEVPHPW
Sbjct: 336 EVEILMKKLNFIPTSVFTTDLQYVDFLDRVHKAELKLRSKNLWEVPHPW 379

BLAST of Carg25675 vs. TAIR 10
Match: AT5G56970.1 (cytokinin oxidase 3 )

HSP 1 Score: 329.3 bits (843), Expect = 4.5e-90
Identity = 161/334 (48.20%), Postives = 233/334 (69.76%), Query Frame = 0

Query: 74  VEFAARDFGNRYQYYPAAILHPSSVSDIAMTIKHIWKMGPGSELT--VAARGHGHSLHGQ 133
           VE AA DFG+  + +P+A+L PSSV DI   I    K+   S+L+  +AARGHGHS  GQ
Sbjct: 55  VESAATDFGHVTKIFPSAVLIPSSVEDITDLI----KLSFDSQLSFPLAARGHGHSHRGQ 114

Query: 134 AQAHQGVVINMESLQGPQMQVVHTRSFKYIDVSGGELWINILQESLKYGLTPKSWTDYLH 193
           A A  GVV+NM S+      +  +R+  Y+DV    LWI +L ++L+ GLTP SWTDYL+
Sbjct: 115 ASAKDGVVVNMRSMVNRDRGIKVSRTCLYVDVDAAWLWIEVLNKTLELGLTPVSWTDYLY 174

Query: 194 LTVGGTLSNAGISGQAFRHGPQISNVHQLEVVTGKGDVVNCSEEQNSDLFYSVLGGLGQF 253
           LTVGGTLSN GISGQ FR+GPQI+NV +++V+TGKG++  CS++ NSDLF++VLGGLGQF
Sbjct: 175 LTVGGTLSNGGISGQTFRYGPQITNVLEMDVITGKGEIATCSKDMNSDLFFAVLGGLGQF 234

Query: 254 GIITRAKILLEPAPTMVKWIRVLYFDFTTFTREQELLISGENTFDYIEGFVIINRTGLLN 313
           GIITRA+I LE AP   KW+R LY DF+ FTR+QE +IS  +  D++EG ++++  G  +
Sbjct: 235 GIITRARIKLEVAPKRAKWLRFLYIDFSEFTRDQERVISKTDGVDFLEGSIMVDH-GPPD 294

Query: 314 NWRSS-FDPQDPVQASQFKSEGKILYCLELAKYFHHNKDNNNVDQEVKRLLSRLSYIPST 373
           NWRS+ + P D ++ +      +++YCLE+ KY+        V++E++ L   L+++   
Sbjct: 295 NWRSTYYPPSDHLRIASMVKRHRVIYCLEVVKYYDET-SQYTVNEEMEELSDSLNHVRGF 354

Query: 374 LFISEVTYVEFLDRVQVSAVKLQSKGLWEVPHPW 405
           ++  +VTY++FL+RV+   + L+SKG W+VPHPW
Sbjct: 355 MYEKDVTYMDFLNRVRTGELNLKSKGQWDVPHPW 382

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7030781.13.0e-253100.00Cytokinin dehydrogenase 6, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022941633.11.7e-23299.26cytokinin dehydrogenase 6-like [Cucurbita moschata][more]
XP_023525504.11.2e-23098.51cytokinin dehydrogenase 6-like [Cucurbita pepo subsp. pepo][more]
XP_022988501.14.7e-23098.02cytokinin dehydrogenase 6-like isoform X1 [Cucurbita maxima][more]
XP_022988509.19.8e-22896.34cytokinin dehydrogenase 6-like isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LY713.9e-14766.92Cytokinin dehydrogenase 6 OS=Arabidopsis thaliana OX=3702 GN=CKX6 PE=2 SV=2[more]
O222132.6e-14365.06Cytokinin dehydrogenase 1 OS=Arabidopsis thaliana OX=3702 GN=CKX1 PE=1 SV=1[more]
Q75K783.0e-13964.25Cytokinin dehydrogenase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX9 PE=2 ... [more]
Q5JLP48.2e-13766.67Cytokinin dehydrogenase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=CKX4 PE=2 ... [more]
Q67YU01.5e-10151.55Cytokinin dehydrogenase 5 OS=Arabidopsis thaliana OX=3702 GN=CKX5 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1FSN08.3e-23399.26Cytokinin dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111446933 PE=3 SV=1[more]
A0A6J1JHC82.3e-23098.02Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1[more]
A0A6J1JLT14.7e-22896.34Cytokinin dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111485722 PE=3 SV=1[more]
A0A6J1CFB12.4e-21191.09Cytokinin dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010228 PE=3 SV=1[more]
A0A1S3BD718.4e-20989.60Cytokinin dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488756 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G63440.12.8e-14866.92cytokinin oxidase/dehydrogenase 6 [more]
AT2G41510.11.9e-14465.06cytokinin oxidase/dehydrogenase 1 [more]
AT1G75450.11.0e-10251.55cytokinin oxidase 5 [more]
AT1G75450.21.4e-10254.73cytokinin oxidase 5 [more]
AT5G56970.14.5e-9048.20cytokinin oxidase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 89..233
e-value: 1.6E-18
score: 66.8
IPR016167FAD-binding, type PCMH, subdomain 1GENE3D3.30.43.10coord: 74..141
e-value: 3.6E-151
score: 505.9
IPR015345Cytokinin dehydrogenase 1, FAD/cytokinin binding domainPFAMPF09265Cytokin-bindcoord: 265..404
e-value: 3.7E-49
score: 167.6
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 142..266
e-value: 3.6E-151
score: 505.9
IPR016170Cytokinin dehydrogenase, C-terminal domain superfamilyGENE3D3.40.462.10coord: 267..405
e-value: 3.6E-151
score: 505.9
NoneNo IPR availablePANTHERPTHR13878GULONOLACTONE OXIDASEcoord: 29..404
NoneNo IPR availablePANTHERPTHR13878:SF53CYTOKININ DEHYDROGENASE 6coord: 29..404
IPR006093Oxygen oxidoreductase covalent FAD-binding sitePROSITEPS00862OX2_COVAL_FADcoord: 89..124
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 85..264
score: 14.103783
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 71..263
IPR016164FAD-linked oxidase-like, C-terminalSUPERFAMILY55103FAD-linked oxidases, C-terminal domaincoord: 265..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25675-RACarg25675-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009690 cytokinin metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0019139 cytokinin dehydrogenase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0003824 catalytic activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity