Carg25661 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25661
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCBL-interacting serine/threonine-protein kinase 1
LocationCarg_Chr06: 9681729 .. 9684850 (+)
RNA-Seq ExpressionCarg25661
SyntenyCarg25661
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAGAGAGCCACAAATTTGGCGGATGGAAATTTAGCAGTGACGAAGAAGAAGAAGACGAAGAAGGAGAATGAGAGTTGGGAAGTATGAGTTGGGGAAAACTCTGGGGGAAGGAAATTTCGGCAAGGTAAAGTTGGCCGTCGATCTCCGTTCTGGCCGCCGTTATGCTGTCAAGATCCTTGACAAGACCAAGATTCTTCATCTCAACTTCTCTGATCAGGTTTGGTTTCATTTCCTTTCATCCTTTTATTTTAGTTCTTATTGTTGTTATTCAGCGAATCGAACTGATGCAATCGGAATCGATTGCAGATTAAGCGTGAAATAAGTACTCTCAAGCTTCTTAGACATCCGAATGTTGTTAGACTCTATGAGGTTTGTGTTCGAATTTGTGTGATTCGTGTGGATTCTATGAATGAACGGGAGATTTGAATGTTTTGTGTATATTCTACAGGTTTTAGCTAGTAAGACGAAGATTTATATGGTTATGGAGTGCGTCACTGGTGGGGAATTGTTTGATAGAATCGTAAGCGTTCTATCTGATGTTGATTTAATTTAGTTATTAGTTGAATTCTTGATCGATAGAGAGAATCGGAGATTTATGTGTGCTGTTTTAGGAATCAAAAGGTAAGTTGGATGAAGCTGAAGGACGACGACTCTTCCAGCAGCTTTTTGATGGTCTAAGCTACTGCCATGATAAAGGAGTTTACCACAGAGATCTCAAGGTATTTACCTGCTATTTCTGTCTCCTTGAATCAAATAATCACTCTTCATAGCGTTTTTATTTAATATTCCTTGTCGGTTGATGATCCATGTAGCTGGAAAACGTGCTTGTTGATGATAAGGGAAACATTAAGATTTCTGATTTTGGCCTCAGTGCTTTACCGCAGAATTGCAGGGTAATTTTATACATATCTACTTTCGAGATCATTTTCATGTAGATCTAGGCTACTTAATTAGCAGTTCAAGAACTAGAATTAATGGATAGTTCATGACATTAGGAAGATGGTTTGCTGCACACCACCTGTGGAAGTCCCAACTATGTTGCACCAGAAGTACTTGCTAATAGAGGATACAATGGAGCTGCCTCTGATATATGGTCATGTGGGGTTATCTTGTATGTGATTCTTACAGCTTGTCTTCCATTTGACGAGACGAATCTGGCTCTACTTTATAAAAAGGTACCTAAAGCTGATTGCGAAAGGGGAAAAAAAAAAACAAACAAAAGGAAGAATCTAAATATGTCTTCTATTTGTTTAGATCTCTGTCTGTATCTCTCTCTCTCCTCCCTTTAGAAAATATATTTATATGCATCAACATTCGTTGTAGACTTTGAGGGGGGATTTTCAGCTACCCAAATGGTTATCAGCAGGTGCCAGAAATTTGATAAAGAGAACTCTTGACCCCAATCCCAATACTCGGATTACAATGGCAGGAATTAAAAAGGATGAATGGTTCAAGATTGACTATAATCCTGCTTCTCCTTGTTATGATGATGATGATGAGGATGAGGATATGCCATTCACTGATCAGGACGATGCCATCTCGGCGCACGATGAGGTATACACAGAGAGATGAAGAGACAATCTATGTTTCTTTCTCCTTGTCTTCTCTCCACCTTTCCCTCTCTGTGTTGTGTTCTCTTAACTGATGGGATTTTGGATGTTACCTTGGTTTAGGCATTTGACGGTGAAAACAGCCCAGAATCTCCCTCTATTATCAACGCGTTTGAGTTAATTGGGATGTCTTCAAGTCTGGACCTGTCCGGCTTCTTTGAGCAAGAGGTGAGAGCTCAAGAGCAGAAGGACTATGCAATCCGTGGCTTGTTTGTTTGTTTATTTAGAACTTCCATTTTATTACTTATCCAAAGCTTATTCGATAATCATTTTGTTTTTATCTTCTAATTTTCGGTAAGCTTTTTACCTTTCTTACAAACATACTTGAATGAAAAACTATACCTGCAAGTACTATTTATAACTTTCGTCAAAATAGTTATCATAAAACCTTAATGTTGTAAGCTGAACAATCACAGGATGTTTCTGAGAGGAAGACCAGATTTACATCCAACCATTCTGCGAAGGACTTGCTCGAGAGGATCGAGATTATAGCAACGGATATGGGATTCCGGGTCCAAAAGAAAAGTGGAAAGGTTGGTACCCAGTTCTTAGAATTGAAAGAATTTGGTAGATGTCAATATCATTAATCACTGCATGAAAATGGATTGATTTGAGCTCTAAGCCATCGTAGTCAGCCATTTTAAATGACAAAGGCCCCTCTATTTGAAACCCTTTGCCATAAGTTACCCACTAACATGCAGTTTCTCTCTCTTTTATGCATCTTCAGTTGAAAGTAATGCAAGAGATAAGAAATCAAAGAAGCCTAAGCAATCTCTCATTCAAAGCAGAGGTAAACATTTCATTAAGCCTTGCATTTAAATTTTGATTGCTCCCATAACCAAACCTGCTTGGTCTCAGGTCTTTGAGATTAGCCCATTGCTTAACGTAGTGGAACTGAAGAAATCCTTTGGAGATTCCTCAGCTTACAGACAGGTACACAATTCATAGCAAGTAAGAAAAACATGAGCTAAAGAATCAGCCTTCTTTACTTCCTAACATTCATTTCAATTCAAACCCTGCAGTTGTGTGAAAGGCTATCAAATGATTTGGGCACAAACCCCGAGCTCGAACGGCATAATTCGAGTTCATTCACGTTAAATAGTACATGTTAGAAGAAAGTGATACCCAGTTATAGGTCTTTGATTAGAATTATTGTTGTGTTTATAGTTTGGGTTGATATCATTTGCTGCCTTTGATTGGTGCATAAGTTGGTTTAAATGTAGATAACAGGAGATAGAGCCAAATATTACATGGCTGGAAGCGTCCCGTGTGTGTCCCCTTGTATGTAATTCGATATCAAATGTAAAAGAACTGAAGTTCATTTGTATGATAGAGGAACTCCATTGTTGTGGCATTCTGGCAATCAATCGGGAAGGAGAGGAGCCACTATTCACTTCACAAAGATGTTAATGATACGCCCGAAATAAAAAAGTCCGAGGAAAAACCTTTGTGGACGTGTTACATCGATCTTACATAACATGCTATCAAATTAAAAAATATTGATCGT

mRNA sequence

AGAGAGAGAGAGCCACAAATTTGGCGGATGGAAATTTAGCAGTGACGAAGAAGAAGAAGACGAAGAAGGAGAATGAGAGTTGGGAAGTATGAGTTGGGGAAAACTCTGGGGGAAGGAAATTTCGGCAAGGTAAAGTTGGCCGTCGATCTCCGTTCTGGCCGCCGTTATGCTGTCAAGATCCTTGACAAGACCAAGATTCTTCATCTCAACTTCTCTGATCAGATTAAGCGTGAAATAAGTACTCTCAAGCTTCTTAGACATCCGAATGTTGTTAGACTCTATGAGGTTTTAGCTAGTAAGACGAAGATTTATATGGTTATGGAGTGCGTCACTGGTGGGGAATTGTTTGATAGAATCGAATCAAAAGGTAAGTTGGATGAAGCTGAAGGACGACGACTCTTCCAGCAGCTTTTTGATGGTCTAAGCTACTGCCATGATAAAGGAGTTTACCACAGAGATCTCAAGCTGGAAAACGTGCTTGTTGATGATAAGGGAAACATTAAGATTTCTGATTTTGGCCTCAGTGCTTTACCGCAGAATTGCAGGGAAGATGGTTTGCTGCACACCACCTGTGGAAGTCCCAACTATGTTGCACCAGAAGTACTTGCTAATAGAGGATACAATGGAGCTGCCTCTGATATATGGTCATGTGGGGTTATCTTGTATGTGATTCTTACAGCTTGTCTTCCATTTGACGAGACGAATCTGGCTCTACTTTATAAAAAGACTTTGAGGGGGGATTTTCAGCTACCCAAATGGTTATCAGCAGGTGCCAGAAATTTGATAAAGAGAACTCTTGACCCCAATCCCAATACTCGGATTACAATGGCAGGAATTAAAAAGGATGAATGGTTCAAGATTGACTATAATCCTGCTTCTCCTTGTTATGATGATGATGATGAGGATGAGGATATGCCATTCACTGATCAGGACGATGCCATCTCGGCGCACGATGAGGCATTTGACGGTGAAAACAGCCCAGAATCTCCCTCTATTATCAACGCGTTTGAGTTAATTGGGATGTCTTCAAGTCTGGACCTGTCCGGCTTCTTTGAGCAAGAGGATGTTTCTGAGAGGAAGACCAGATTTACATCCAACCATTCTGCGAAGGACTTGCTCGAGAGGATCGAGATTATAGCAACGGATATGGGATTCCGGGTCCAAAAGAAAAGTGGAAAGTTGAAAGTAATGCAAGAGATAAGAAATCAAAGAAGCCTAAGCAATCTCTCATTCAAAGCAGAGGTCTTTGAGATTAGCCCATTGCTTAACGTAGTGGAACTGAAGAAATCCTTTGGAGATTCCTCAGCTTACAGACAGTTGTGTGAAAGGCTATCAAATGATTTGGGCACAAACCCCGAGCTCGAACGGCATAATTCGAGTTCATTCACGTTAAATAGTACATGTTAGAAGAAAGTGATACCCAGTTATAGGTCTTTGATTAGAATTATTGTTGTGTTTATAGTTTGGGTTGATATCATTTGCTGCCTTTGATTGGTGCATAAGTTGGTTTAAATGTAGATAACAGGAGATAGAGCCAAATATTACATGGCTGGAAGCGTCCCGTGTGTGTCCCCTTGTATGTAATTCGATATCAAATGTAAAAGAACTGAAGTTCATTTGTATGATAGAGGAACTCCATTGTTGTGGCATTCTGGCAATCAATCGGGAAGGAGAGGAGCCACTATTCACTTCACAAAGATGTTAATGATACGCCCGAAATAAAAAAGTCCGAGGAAAAACCTTTGTGGACGTGTTACATCGATCTTACATAACATGCTATCAAATTAAAAAATATTGATCGT

Coding sequence (CDS)

ATGAGAGTTGGGAAGTATGAGTTGGGGAAAACTCTGGGGGAAGGAAATTTCGGCAAGGTAAAGTTGGCCGTCGATCTCCGTTCTGGCCGCCGTTATGCTGTCAAGATCCTTGACAAGACCAAGATTCTTCATCTCAACTTCTCTGATCAGATTAAGCGTGAAATAAGTACTCTCAAGCTTCTTAGACATCCGAATGTTGTTAGACTCTATGAGGTTTTAGCTAGTAAGACGAAGATTTATATGGTTATGGAGTGCGTCACTGGTGGGGAATTGTTTGATAGAATCGAATCAAAAGGTAAGTTGGATGAAGCTGAAGGACGACGACTCTTCCAGCAGCTTTTTGATGGTCTAAGCTACTGCCATGATAAAGGAGTTTACCACAGAGATCTCAAGCTGGAAAACGTGCTTGTTGATGATAAGGGAAACATTAAGATTTCTGATTTTGGCCTCAGTGCTTTACCGCAGAATTGCAGGGAAGATGGTTTGCTGCACACCACCTGTGGAAGTCCCAACTATGTTGCACCAGAAGTACTTGCTAATAGAGGATACAATGGAGCTGCCTCTGATATATGGTCATGTGGGGTTATCTTGTATGTGATTCTTACAGCTTGTCTTCCATTTGACGAGACGAATCTGGCTCTACTTTATAAAAAGACTTTGAGGGGGGATTTTCAGCTACCCAAATGGTTATCAGCAGGTGCCAGAAATTTGATAAAGAGAACTCTTGACCCCAATCCCAATACTCGGATTACAATGGCAGGAATTAAAAAGGATGAATGGTTCAAGATTGACTATAATCCTGCTTCTCCTTGTTATGATGATGATGATGAGGATGAGGATATGCCATTCACTGATCAGGACGATGCCATCTCGGCGCACGATGAGGCATTTGACGGTGAAAACAGCCCAGAATCTCCCTCTATTATCAACGCGTTTGAGTTAATTGGGATGTCTTCAAGTCTGGACCTGTCCGGCTTCTTTGAGCAAGAGGATGTTTCTGAGAGGAAGACCAGATTTACATCCAACCATTCTGCGAAGGACTTGCTCGAGAGGATCGAGATTATAGCAACGGATATGGGATTCCGGGTCCAAAAGAAAAGTGGAAAGTTGAAAGTAATGCAAGAGATAAGAAATCAAAGAAGCCTAAGCAATCTCTCATTCAAAGCAGAGGTCTTTGAGATTAGCCCATTGCTTAACGTAGTGGAACTGAAGAAATCCTTTGGAGATTCCTCAGCTTACAGACAGTTGTGTGAAAGGCTATCAAATGATTTGGGCACAAACCCCGAGCTCGAACGGCATAATTCGAGTTCATTCACGTTAAATAGTACATGTTAG

Protein sequence

MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKLLRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCHDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANRGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRTLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMGFRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERLSNDLGTNPELERHNSSSFTLNSTC
Homology
BLAST of Carg25661 vs. NCBI nr
Match: KAG7029041.1 (CBL-interacting serine/threonine-protein kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 885.6 bits (2287), Expect = 1.8e-253
Identity = 444/444 (100.00%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPELERHNSSSFTLNSTC
Sbjct: 421 SNDLGTNPELERHNSSSFTLNSTC 444

BLAST of Carg25661 vs. NCBI nr
Match: XP_022972049.1 (CBL-interacting serine/threonine-protein kinase 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 882.5 bits (2279), Expect = 1.5e-252
Identity = 442/444 (99.55%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDED+PFTDQDDAISAHDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDIPFTDQDDAISAHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPEL+RHNSSSFTLNSTC
Sbjct: 421 SNDLGTNPELKRHNSSSFTLNSTC 444

BLAST of Carg25661 vs. NCBI nr
Match: KAG6597598.1 (CBL-interacting serine/threonine-protein kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.6 bits (2274), Expect = 5.8e-252
Identity = 444/446 (99.55%), Postives = 444/446 (99.55%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDD--DEDEDMPFTDQDDAISAHDEAFD 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDD  DEDEDMPFTDQDDAISAHDEAFD
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDEDMPFTDQDDAISAHDEAFD 300

Query: 301 GENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATD 360
           GENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATD
Sbjct: 301 GENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATD 360

Query: 361 MGFRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCE 420
           MGFRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCE
Sbjct: 361 MGFRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCE 420

Query: 421 RLSNDLGTNPELERHNSSSFTLNSTC 445
           RLSNDLGTNPELERHNSSSFTLNSTC
Sbjct: 421 RLSNDLGTNPELERHNSSSFTLNSTC 446

BLAST of Carg25661 vs. NCBI nr
Match: XP_023540923.1 (CBL-interacting serine/threonine-protein kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 879.0 bits (2270), Expect = 1.7e-251
Identity = 440/444 (99.10%), Postives = 442/444 (99.55%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDE++PFTDQDDAIS HDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEEIPFTDQDDAISTHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPELERHNSSSFTLNS C
Sbjct: 421 SNDLGTNPELERHNSSSFTLNSAC 444

BLAST of Carg25661 vs. NCBI nr
Match: XP_022935693.1 (CBL-interacting serine/threonine-protein kinase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 877.5 bits (2266), Expect = 4.9e-251
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCY DDDEDED+PFTDQDDAISAHDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCY-DDDEDEDIPFTDQDDAISAHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPELERHNSSSFTLNSTC
Sbjct: 421 SNDLGTNPELERHNSSSFTLNSTC 443

BLAST of Carg25661 vs. ExPASy Swiss-Prot
Match: Q8RWC9 (CBL-interacting serine/threonine-protein kinase 1 OS=Arabidopsis thaliana OX=3702 GN=CIPK1 PE=1 SV=2)

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-166
Identity = 302/432 (69.91%), Postives = 351/432 (81.25%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MR+GKYELG+TLGEGNFGKVK A D  SG  +AVKI+DK++I  LNFS QIKREI TLK+
Sbjct: 15  MRLGKYELGRTLGEGNFGKVKFAKDTVSGHSFAVKIIDKSRIADLNFSLQIKREIRTLKM 74

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           L+HP++VRL+EVLASKTKI MVME VTGGELFDRI S GKL E +GR++FQQL DG+SYC
Sbjct: 75  LKHPHIVRLHEVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYC 134

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H KGV+HRDLKLENVL+D KG+IKI+DFGLSALPQ+ R+DGLLHTTCGSPNYVAPEVLAN
Sbjct: 135 HSKGVFHRDLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLAN 194

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGY+GAASDIWSCGVILYVILT CLPFD+ NLA+LY+K  +GD  +P+WLS GAR +IKR
Sbjct: 195 RGYDGAASDIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKR 254

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
            LDPNP TRIT+ GIK  EWFK++Y P+ P  DDDDE+E       DDA S  +   +  
Sbjct: 255 MLDPNPVTRITVVGIKASEWFKLEYIPSIP--DDDDEEE---VDTDDDAFSIQELGSEEG 314

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
              +SP+IINAF+LIGMSS LDLSGFFEQE+VSER+ RFTSN SAKDLLE+IE   T+MG
Sbjct: 315 KGSDSPTIINAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMG 374

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           F VQKK  KL+V QE RNQ+    LS  AEVFEI P LNVVEL+KS+GDS  YRQL ERL
Sbjct: 375 FSVQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERL 434

Query: 421 SNDLGT-NPELE 432
             D+GT +PE E
Sbjct: 435 LKDVGTSSPEQE 441

BLAST of Carg25661 vs. ExPASy Swiss-Prot
Match: Q94C40 (CBL-interacting serine/threonine-protein kinase 17 OS=Arabidopsis thaliana OX=3702 GN=CIPK17 PE=1 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 3.2e-160
Identity = 285/431 (66.13%), Postives = 349/431 (80.97%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELG+TLGEGN  KVK A+D  +G  +A+KI++K+ I  LN S QIKREI TLK+
Sbjct: 6   MRVGKYELGRTLGEGNSAKVKFAIDTLTGESFAIKIIEKSCITRLNVSFQIKREIRTLKV 65

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           L+HPN+VRL+EVLASKTKIYMV+ECVTGG+LFDRI SKGKL E +GR++FQQL DG+SYC
Sbjct: 66  LKHPNIVRLHEVLASKTKIYMVLECVTGGDLFDRIVSKGKLSETQGRKMFQQLIDGVSYC 125

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H+KGV+HRDLKLENVL+D KG+IKI+DFGLSAL Q+ REDGLLHTTCGSPNYVAPEVLAN
Sbjct: 126 HNKGVFHRDLKLENVLLDAKGHIKITDFGLSALSQHYREDGLLHTTCGSPNYVAPEVLAN 185

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
            GY+GAASDIWSCGVILYVILT CLPFD+ NLA++ +K  +GD  +P+W+S GA+ +IKR
Sbjct: 186 EGYDGAASDIWSCGVILYVILTGCLPFDDANLAVICRKIFKGDPPIPRWISLGAKTMIKR 245

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
            LDPNP TR+T+AGIK  +WFK DY P++  YDDDD+     +  Q+D     +  ++ E
Sbjct: 246 MLDPNPVTRVTIAGIKAHDWFKHDYTPSN--YDDDDD----VYLIQEDVFMMKE--YEEE 305

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
            SP+SP+IINAF+LIGMSS LDLSGFFE E +SER+ RFTSN  AKDLLE IE I T+MG
Sbjct: 306 KSPDSPTIINAFQLIGMSSFLDLSGFFETEKLSERQIRFTSNSLAKDLLENIETIFTEMG 365

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           F +QKK  KLK ++E   Q+    LS  AEVFEISP LNVVEL+KS GDSS Y+QL ERL
Sbjct: 366 FCLQKKHAKLKAIKEESTQKRQCGLSVTAEVFEISPSLNVVELRKSHGDSSLYKQLYERL 425

Query: 421 SNDLGTNPELE 432
            N+LG++ +++
Sbjct: 426 LNELGSSSQVQ 428

BLAST of Carg25661 vs. ExPASy Swiss-Prot
Match: Q9LGV5 (CBL-interacting protein kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK1 PE=2 SV=1)

HSP 1 Score: 534.6 bits (1376), Expect = 1.0e-150
Identity = 279/448 (62.28%), Postives = 336/448 (75.00%), Query Frame = 0

Query: 3   VGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKLLR 62
           +G+YE+G+TLGEGNFGKVK A  L +G  +A+KILD+ KIL L F DQI+REI TLKLL+
Sbjct: 16  LGRYEIGRTLGEGNFGKVKYARHLATGAHFAIKILDRNKILSLRFDDQIRREIGTLKLLK 75

Query: 63  HPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCHD 122
           HPNVVRL+EV ASKTKIYMV+E V GGELFD+I  KGKL E EGRRLFQQL D +SYCHD
Sbjct: 76  HPNVVRLHEVAASKTKIYMVLEYVNGGELFDKIAVKGKLSEHEGRRLFQQLIDAVSYCHD 135

Query: 123 KGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANRG 182
           KGVYHRDLK ENVLVD +GNIKISDFGLSALPQ+   DGLLHTTCGSPNY+APEVL NRG
Sbjct: 136 KGVYHRDLKPENVLVDRRGNIKISDFGLSALPQHLGNDGLLHTTCGSPNYIAPEVLQNRG 195

Query: 183 YNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRTL 242
           Y+G+ SDIWSCGVILYV+L   LPFD+ NL +LY+K  +GD Q+PKWLS  AR+L++R L
Sbjct: 196 YDGSLSDIWSCGVILYVMLVGYLPFDDRNLVVLYQKIFKGDTQIPKWLSPSARDLLRRIL 255

Query: 243 DPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDE---DEDMPFTDQDDAISAHDEAFDG 302
           +PNP  RI +AGIK+ EWF+ DY P  P YDDDD+   D  +P  +Q D           
Sbjct: 256 EPNPMKRINIAGIKEHEWFQKDYTPVVP-YDDDDDNYLDSVLPIKEQID----------- 315

Query: 303 ENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDM 362
           E   E P+ INAF+LIGM+S+LDLSGFFE+ED S+RK RFTS HS KDL ++IE + T+M
Sbjct: 316 EAKQEKPTHINAFQLIGMASALDLSGFFEEEDASQRKIRFTSTHSPKDLFDKIENVVTEM 375

Query: 363 GFRVQKKSGKLKVMQEIRNQRSLSNLS---FKAEVFEISPLLNVVELKKSFGDSSAYRQL 422
           GF+VQ+ + KLKVM+  R  ++L N S      EV E+ P L VVELKKS GD   YRQL
Sbjct: 376 GFQVQRGNSKLKVMKNGRGSKNLRNPSSFLVCTEVVELGPSLYVVELKKSHGDPILYRQL 435

Query: 423 CERLSNDLGT--NPELERHNSSSFTLNS 443
           CERLS++LG     +++R  S    L S
Sbjct: 436 CERLSDELGVCKTEQIQRTESLEDDLES 451

BLAST of Carg25661 vs. ExPASy Swiss-Prot
Match: Q0D4B2 (CBL-interacting protein kinase 21 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK21 PE=2 SV=2)

HSP 1 Score: 529.6 bits (1363), Expect = 3.3e-149
Identity = 275/431 (63.81%), Postives = 329/431 (76.33%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MR+GKYE+G+ LGEG+FGKVKLA    +G  +A+KILD+ +IL +   +QIKREI+TLKL
Sbjct: 82  MRMGKYEMGRALGEGHFGKVKLARHADTGAAFAIKILDRQRILAMKIDEQIKREIATLKL 141

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           L+HPNVVRL+EV ASKTKIYMV+E V GGELFD+I  KGKL E EGR+LFQQL D +SYC
Sbjct: 142 LKHPNVVRLHEVSASKTKIYMVLEYVNGGELFDKIALKGKLSEKEGRKLFQQLMDAVSYC 201

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H+KGVYHRDLK ENVLVD KGNIK+SDFGLSALPQN R+DGLLHTTCGSPNY+APEVL N
Sbjct: 202 HEKGVYHRDLKPENVLVDAKGNIKVSDFGLSALPQNQRKDGLLHTTCGSPNYIAPEVLLN 261

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGY+G+ SDIWSCGVILYV+LT  LPFD+ N  +LY+K L+GD ++PKWLS GA++++++
Sbjct: 262 RGYDGSLSDIWSCGVILYVMLTGNLPFDDQNTVVLYQKILKGDARIPKWLSPGAQDILRK 321

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDD---EDEDMPFTDQDDAISAHDEAF 300
            LDPNP TR+ + GI+  EWF+ DY PA P  DDDD    D ++  T+  D         
Sbjct: 322 ILDPNPITRLDITGIRAHEWFRQDYTPAMPFDDDDDNNISDGNLHMTENQDI-------- 381

Query: 301 DGENSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIAT 360
             E SP + S INAF+LIGMSS LDLSGFFE+EDVSERK RF SN+S   L E+IE   T
Sbjct: 382 --ETSP-AISQINAFQLIGMSSCLDLSGFFEKEDVSERKIRFVSNYSPTSLFEKIESTVT 441

Query: 361 DMGFRVQKKSGKLKVMQ---EIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYR 420
           + GF+VQK SGKLKV+Q   E  N R   NL   AEVFEI+  L VVELK+S GD S YR
Sbjct: 442 EKGFQVQKNSGKLKVIQVCKEPANPRGHGNLLISAEVFEINESLYVVELKRSSGDCSLYR 501

Query: 421 QLCERLSNDLG 426
           QLC  LS DLG
Sbjct: 502 QLCASLSEDLG 501

BLAST of Carg25661 vs. ExPASy Swiss-Prot
Match: Q75L42 (CBL-interacting protein kinase 17 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK17 PE=2 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 1.8e-147
Identity = 264/423 (62.41%), Postives = 325/423 (76.83%), Query Frame = 0

Query: 3   VGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKLLR 62
           +G YE+G+TLGEGNFGKVK A  L +G  +AVKILD+ +++ L   DQI+REI+TLKLLR
Sbjct: 10  LGGYEMGRTLGEGNFGKVKYARHLATGGHFAVKILDRGRVVSLRAGDQIRREIATLKLLR 69

Query: 63  HPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCHD 122
           HP+VVRL+EV ASKTKIYMV+E V GGELF+RI  KGKL E EGRRLFQQL DG+SYCHD
Sbjct: 70  HPHVVRLHEVAASKTKIYMVLEFVNGGELFERIAVKGKLSEKEGRRLFQQLIDGVSYCHD 129

Query: 123 KGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANRG 182
           +GVYHRDLK ENVLVD KGNIKISDFGLSALPQ+   DGLLHTTCGSPNY+APEVL N+G
Sbjct: 130 RGVYHRDLKPENVLVDQKGNIKISDFGLSALPQHLGNDGLLHTTCGSPNYIAPEVLQNKG 189

Query: 183 YNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRTL 242
           Y+G+ SDIWSCGVILYV+L   LPFD+ N+ +LY+K  +GD Q+PKWLS  A+NL++R L
Sbjct: 190 YDGSLSDIWSCGVILYVMLIGYLPFDDRNIVVLYQKIFKGDTQIPKWLSHSAQNLLRRIL 249

Query: 243 DPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGENS 302
           +PNP  RI MAGIK  EWF+ DY P  P YDDDDED       Q  A     E  + E  
Sbjct: 250 EPNPMKRIDMAGIKSHEWFQKDYIPVLP-YDDDDEDV------QFGARLPAKEQINDEPG 309

Query: 303 PESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMGFR 362
            ++   INAF+LIGM+SSLDLSGFFE E+VS+R+ RFTS H  KD  ++IE  AT++GF+
Sbjct: 310 DKNSHQINAFQLIGMASSLDLSGFFEDEEVSQRRIRFTSTHPPKDAFDKIESSATELGFQ 369

Query: 363 VQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERLSN 422
           VQ+   KLK+M+  +  ++  +    AEVFE+ P +NVVEL+KS GD + YRQLCER+S+
Sbjct: 370 VQRGHSKLKLMRNCKGSKNPESFMVSAEVFELGPSVNVVELRKSNGDPALYRQLCERISS 425

Query: 423 DLG 426
           D+G
Sbjct: 430 DMG 425

BLAST of Carg25661 vs. ExPASy TrEMBL
Match: A0A6J1IAC0 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111470695 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 7.4e-253
Identity = 442/444 (99.55%), Postives = 444/444 (100.00%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDED+PFTDQDDAISAHDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDIPFTDQDDAISAHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPEL+RHNSSSFTLNSTC
Sbjct: 421 SNDLGTNPELKRHNSSSFTLNSTC 444

BLAST of Carg25661 vs. ExPASy TrEMBL
Match: A0A6J1FB81 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111442472 PE=3 SV=1)

HSP 1 Score: 877.5 bits (2266), Expect = 2.4e-251
Identity = 442/444 (99.55%), Postives = 443/444 (99.77%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRITMAGIKKDEWFKIDYNPASPCY DDDEDED+PFTDQDDAISAHDEAFDGE
Sbjct: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCY-DDDEDEDIPFTDQDDAISAHDEAFDGE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPELERHNSSSFTLNSTC
Sbjct: 421 SNDLGTNPELERHNSSSFTLNSTC 443

BLAST of Carg25661 vs. ExPASy TrEMBL
Match: A0A6J1IRR4 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111479391 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.9e-228
Identity = 402/444 (90.54%), Postives = 420/444 (94.59%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVG YELGKTLGEGNFGKVKLA D+R+GRRYAVKILDKT+IL LNFSDQIKREISTLKL
Sbjct: 1   MRVGNYELGKTLGEGNFGKVKLAADIRTGRRYAVKILDKTRILRLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMV+EC +GGELFDRIESKGK+DEAEGRRLFQQL DGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVLECASGGELFDRIESKGKMDEAEGRRLFQQLIDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H+KGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HEKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTACLPFDETNL+LLYKKTLRGDFQ+PKWLSAGAR LI R
Sbjct: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLSLLYKKTLRGDFQIPKWLSAGARKLIGR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRIT+AGIK++EWFK DYNPASPCYDD++E    P  DQDDAIS  DE  D E
Sbjct: 241 TLDPNPNTRITIAGIKENEWFKTDYNPASPCYDDEEEG---PLDDQDDAISTPDEPSDVE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSP+SPSIINAFELIGMSSSLDLSGFFEQEDVSERK RFTSNHSAKDLLERIEIIATDMG
Sbjct: 301 NSPKSPSIINAFELIGMSSSLDLSGFFEQEDVSERKIRFTSNHSAKDLLERIEIIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLKVMQE+R QRSLSNLSF AEVFEISPLL+VVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKVMQEVRCQRSLSNLSFVAEVFEISPLLHVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           SNDLGTNPELERHNSSS  +NSTC
Sbjct: 421 SNDLGTNPELERHNSSSLAVNSTC 441

BLAST of Carg25661 vs. ExPASy TrEMBL
Match: A0A1S3AXS9 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103483933 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.9e-228
Identity = 401/444 (90.32%), Postives = 424/444 (95.50%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MR+GKYELGKTLGEGNFGKVKLA D+R+G RYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRLGKYELGKTLGEGNFGKVKLAADVRTGCRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMV+EC +GGELFDRIESKGK+DEAEGRR+FQQL DGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVLECASGGELFDRIESKGKMDEAEGRRIFQQLIDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVD KGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDAKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNG ASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQ+PKWLSAGARNLI+R
Sbjct: 181 RGYNGVASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQIPKWLSAGARNLIRR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNP+TRIT+AGIK++EWFK DYNPASPCYDDD+E+  +  TDQDDAIS  DE  D E
Sbjct: 241 TLDPNPSTRITIAGIKENEWFKTDYNPASPCYDDDEEEGSL--TDQDDAISTPDEPSDVE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIE+IATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEVIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQKKSGKLK++QEIR+Q+S SNLSF AEVFEISPLL+VVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKKSGKLKLIQEIRSQKSPSNLSFVAEVFEISPLLHVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           S+DLGTNPELER NSSSF LNSTC
Sbjct: 421 SSDLGTNPELERQNSSSFALNSTC 442

BLAST of Carg25661 vs. ExPASy TrEMBL
Match: A0A0A0L993 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3G171200 PE=3 SV=1)

HSP 1 Score: 795.0 bits (2052), Expect = 1.6e-226
Identity = 400/444 (90.09%), Postives = 422/444 (95.05%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELGKTLGEGNFGKVKLA D+R+G RYAVKILDKTKILHLNFSDQIKREISTLKL
Sbjct: 1   MRVGKYELGKTLGEGNFGKVKLAADVRTGCRYAVKILDKTKILHLNFSDQIKREISTLKL 60

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           LRHPNVVRLYEVLASKTKIYMVMEC +GGELFDRIESKGK+DEAEGRR+FQQL DGLSYC
Sbjct: 61  LRHPNVVRLYEVLASKTKIYMVMECASGGELFDRIESKGKMDEAEGRRIFQQLIDGLSYC 120

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           HDKGVYHRDLKLENVLVD KGNIKISDFGLSALP+NCREDGLLHTTCGSPNYVAPEVLAN
Sbjct: 121 HDKGVYHRDLKLENVLVDAKGNIKISDFGLSALPKNCREDGLLHTTCGSPNYVAPEVLAN 180

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGYNGAASDIWSCGVILYVILTA LPFDETNLALLYKKTLRGDFQ+PKWLSAGARNLI+R
Sbjct: 181 RGYNGAASDIWSCGVILYVILTAFLPFDETNLALLYKKTLRGDFQIPKWLSAGARNLIRR 240

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
           TLDPNPNTRIT+AGIK++EWFK DYNPASPCYDDD+E+  +  TDQDDAIS  DE  D E
Sbjct: 241 TLDPNPNTRITIAGIKENEWFKTDYNPASPCYDDDEEEGSL--TDQDDAISTLDEPSDAE 300

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
           NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERK RFTSN SAKDLLERIE+IATDMG
Sbjct: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKIRFTSNRSAKDLLERIEVIATDMG 360

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           FRVQK+SGKLK++QEIR+Q+SLSNLSF AEVFEI PLL+VVELKKSFGDSSAYRQLCERL
Sbjct: 361 FRVQKRSGKLKLIQEIRSQKSLSNLSFVAEVFEIRPLLHVVELKKSFGDSSAYRQLCERL 420

Query: 421 SNDLGTNPELERHNSSSFTLNSTC 445
           S+DLGTNPELER NSSSF LNSTC
Sbjct: 421 SSDLGTNPELERQNSSSFALNSTC 442

BLAST of Carg25661 vs. TAIR 10
Match: AT3G17510.1 (CBL-interacting protein kinase 1 )

HSP 1 Score: 587.0 bits (1512), Expect = 1.2e-167
Identity = 302/432 (69.91%), Postives = 351/432 (81.25%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MR+GKYELG+TLGEGNFGKVK A D  SG  +AVKI+DK++I  LNFS QIKREI TLK+
Sbjct: 15  MRLGKYELGRTLGEGNFGKVKFAKDTVSGHSFAVKIIDKSRIADLNFSLQIKREIRTLKM 74

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           L+HP++VRL+EVLASKTKI MVME VTGGELFDRI S GKL E +GR++FQQL DG+SYC
Sbjct: 75  LKHPHIVRLHEVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYC 134

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H KGV+HRDLKLENVL+D KG+IKI+DFGLSALPQ+ R+DGLLHTTCGSPNYVAPEVLAN
Sbjct: 135 HSKGVFHRDLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLAN 194

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
           RGY+GAASDIWSCGVILYVILT CLPFD+ NLA+LY+K  +GD  +P+WLS GAR +IKR
Sbjct: 195 RGYDGAASDIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKR 254

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
            LDPNP TRIT+ GIK  EWFK++Y P+ P  DDDDE+E       DDA S  +   +  
Sbjct: 255 MLDPNPVTRITVVGIKASEWFKLEYIPSIP--DDDDEEE---VDTDDDAFSIQELGSEEG 314

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
              +SP+IINAF+LIGMSS LDLSGFFEQE+VSER+ RFTSN SAKDLLE+IE   T+MG
Sbjct: 315 KGSDSPTIINAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMG 374

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           F VQKK  KL+V QE RNQ+    LS  AEVFEI P LNVVEL+KS+GDS  YRQL ERL
Sbjct: 375 FSVQKKHAKLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERL 434

Query: 421 SNDLGT-NPELE 432
             D+GT +PE E
Sbjct: 435 LKDVGTSSPEQE 441

BLAST of Carg25661 vs. TAIR 10
Match: AT1G48260.1 (CBL-interacting protein kinase 17 )

HSP 1 Score: 566.2 bits (1458), Expect = 2.3e-161
Identity = 285/431 (66.13%), Postives = 349/431 (80.97%), Query Frame = 0

Query: 1   MRVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKL 60
           MRVGKYELG+TLGEGN  KVK A+D  +G  +A+KI++K+ I  LN S QIKREI TLK+
Sbjct: 6   MRVGKYELGRTLGEGNSAKVKFAIDTLTGESFAIKIIEKSCITRLNVSFQIKREIRTLKV 65

Query: 61  LRHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYC 120
           L+HPN+VRL+EVLASKTKIYMV+ECVTGG+LFDRI SKGKL E +GR++FQQL DG+SYC
Sbjct: 66  LKHPNIVRLHEVLASKTKIYMVLECVTGGDLFDRIVSKGKLSETQGRKMFQQLIDGVSYC 125

Query: 121 HDKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLAN 180
           H+KGV+HRDLKLENVL+D KG+IKI+DFGLSAL Q+ REDGLLHTTCGSPNYVAPEVLAN
Sbjct: 126 HNKGVFHRDLKLENVLLDAKGHIKITDFGLSALSQHYREDGLLHTTCGSPNYVAPEVLAN 185

Query: 181 RGYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKR 240
            GY+GAASDIWSCGVILYVILT CLPFD+ NLA++ +K  +GD  +P+W+S GA+ +IKR
Sbjct: 186 EGYDGAASDIWSCGVILYVILTGCLPFDDANLAVICRKIFKGDPPIPRWISLGAKTMIKR 245

Query: 241 TLDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGE 300
            LDPNP TR+T+AGIK  +WFK DY P++  YDDDD+     +  Q+D     +  ++ E
Sbjct: 246 MLDPNPVTRVTIAGIKAHDWFKHDYTPSN--YDDDDD----VYLIQEDVFMMKE--YEEE 305

Query: 301 NSPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMG 360
            SP+SP+IINAF+LIGMSS LDLSGFFE E +SER+ RFTSN  AKDLLE IE I T+MG
Sbjct: 306 KSPDSPTIINAFQLIGMSSFLDLSGFFETEKLSERQIRFTSNSLAKDLLENIETIFTEMG 365

Query: 361 FRVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERL 420
           F +QKK  KLK ++E   Q+    LS  AEVFEISP LNVVEL+KS GDSS Y+QL ERL
Sbjct: 366 FCLQKKHAKLKAIKEESTQKRQCGLSVTAEVFEISPSLNVVELRKSHGDSSLYKQLYERL 425

Query: 421 SNDLGTNPELE 432
            N+LG++ +++
Sbjct: 426 LNELGSSSQVQ 428

BLAST of Carg25661 vs. TAIR 10
Match: AT3G17510.2 (CBL-interacting protein kinase 1 )

HSP 1 Score: 489.2 bits (1258), Expect = 3.5e-138
Identity = 252/364 (69.23%), Postives = 292/364 (80.22%), Query Frame = 0

Query: 69  LYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCHDKGVYHR 128
           + +VLASKTKI MVME VTGGELFDRI S GKL E +GR++FQQL DG+SYCH KGV+HR
Sbjct: 3   IMQVLASKTKINMVMELVTGGELFDRIVSNGKLTETDGRKMFQQLIDGISYCHSKGVFHR 62

Query: 129 DLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANRGYNGAAS 188
           DLKLENVL+D KG+IKI+DFGLSALPQ+ R+DGLLHTTCGSPNYVAPEVLANRGY+GAAS
Sbjct: 63  DLKLENVLLDAKGHIKITDFGLSALPQHFRDDGLLHTTCGSPNYVAPEVLANRGYDGAAS 122

Query: 189 DIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRTLDPNPNT 248
           DIWSCGVILYVILT CLPFD+ NLA+LY+K  +GD  +P+WLS GAR +IKR LDPNP T
Sbjct: 123 DIWSCGVILYVILTGCLPFDDRNLAVLYQKICKGDPPIPRWLSPGARTMIKRMLDPNPVT 182

Query: 249 RITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGENSPESPSI 308
           RIT+ GIK  EWFK++Y P+ P  DDDDE+E       DDA S  +   +     +SP+I
Sbjct: 183 RITVVGIKASEWFKLEYIPSIP--DDDDEEE---VDTDDDAFSIQELGSEEGKGSDSPTI 242

Query: 309 INAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMGFRVQKKSG 368
           INAF+LIGMSS LDLSGFFEQE+VSER+ RFTSN SAKDLLE+IE   T+MGF VQKK  
Sbjct: 243 INAFQLIGMSSFLDLSGFFEQENVSERRIRFTSNSSAKDLLEKIETAVTEMGFSVQKKHA 302

Query: 369 KLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERLSNDLGT-N 428
           KL+V QE RNQ+    LS  AEVFEI P LNVVEL+KS+GDS  YRQL ERL  D+GT +
Sbjct: 303 KLRVKQEERNQKGQVGLSVTAEVFEIKPSLNVVELRKSYGDSCLYRQLYERLLKDVGTSS 361

Query: 429 PELE 432
           PE E
Sbjct: 363 PEQE 361

BLAST of Carg25661 vs. TAIR 10
Match: AT2G26980.3 (CBL-interacting protein kinase 3 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.8e-121
Identity = 221/438 (50.46%), Postives = 305/438 (69.63%), Query Frame = 0

Query: 2   RVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKLL 61
           RVGKYE+G+T+GEG F KVK A +  +G   A+KILDK K+L    ++QI+REI+T+KL+
Sbjct: 10  RVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATMKLI 69

Query: 62  RHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCH 121
           +HPNVV+LYEV+ASKTKI++++E VTGGELFD+I + G++ E E RR FQQL   + YCH
Sbjct: 70  KHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVNDGRMKEDEARRYFQQLIHAVDYCH 129

Query: 122 DKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANR 181
            +GVYHRDLK EN+L+D  GN+KISDFGLSAL Q  R+DGLLHT+CG+PNYVAPEVL +R
Sbjct: 130 SRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLHTSCGTPNYVAPEVLNDR 189

Query: 182 GYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRT 241
           GY+GA +D+WSCGV+LYV+L   LPFD++NL  LYKK   G+F  P WLS GA  LI R 
Sbjct: 190 GYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEFNCPPWLSLGAMKLITRI 249

Query: 242 LDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGEN 301
           LDPNP TR+T   + +DEWFK DY P  P +++ D   D    D D      +E    E 
Sbjct: 250 LDPNPMTRVTPQEVFEDEWFKKDYKP--PVFEERD---DSNMDDIDAVFKDSEEHLVTEK 309

Query: 302 SPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMGF 361
             E P+ INAFE+I MS  L+L   F+ E   +R+TR T    A +++E+IE  A  +GF
Sbjct: 310 REEQPAAINAFEIISMSRGLNLENLFDPEQEFKRETRITLRGGANEIIEKIEEAAKPLGF 369

Query: 362 RVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERLS 421
            VQKK+ K++ ++ ++  R   NL+   E+F+++P L++V++ KS GD+  + +  ++LS
Sbjct: 370 DVQKKNYKMR-LENVKAGRK-GNLNVATEIFQVAPSLHMVQVSKSKGDTLEFHKFYKKLS 429

Query: 422 NDL----GTNPELERHNS 436
           N L     TN E+++  +
Sbjct: 430 NSLEQVVWTNNEVKKETA 440

BLAST of Carg25661 vs. TAIR 10
Match: AT2G26980.4 (CBL-interacting protein kinase 3 )

HSP 1 Score: 433.7 bits (1114), Expect = 1.8e-121
Identity = 221/438 (50.46%), Postives = 305/438 (69.63%), Query Frame = 0

Query: 2   RVGKYELGKTLGEGNFGKVKLAVDLRSGRRYAVKILDKTKILHLNFSDQIKREISTLKLL 61
           RVGKYE+G+T+GEG F KVK A +  +G   A+KILDK K+L    ++QI+REI+T+KL+
Sbjct: 20  RVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATMKLI 79

Query: 62  RHPNVVRLYEVLASKTKIYMVMECVTGGELFDRIESKGKLDEAEGRRLFQQLFDGLSYCH 121
           +HPNVV+LYEV+ASKTKI++++E VTGGELFD+I + G++ E E RR FQQL   + YCH
Sbjct: 80  KHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVNDGRMKEDEARRYFQQLIHAVDYCH 139

Query: 122 DKGVYHRDLKLENVLVDDKGNIKISDFGLSALPQNCREDGLLHTTCGSPNYVAPEVLANR 181
            +GVYHRDLK EN+L+D  GN+KISDFGLSAL Q  R+DGLLHT+CG+PNYVAPEVL +R
Sbjct: 140 SRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLHTSCGTPNYVAPEVLNDR 199

Query: 182 GYNGAASDIWSCGVILYVILTACLPFDETNLALLYKKTLRGDFQLPKWLSAGARNLIKRT 241
           GY+GA +D+WSCGV+LYV+L   LPFD++NL  LYKK   G+F  P WLS GA  LI R 
Sbjct: 200 GYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEFNCPPWLSLGAMKLITRI 259

Query: 242 LDPNPNTRITMAGIKKDEWFKIDYNPASPCYDDDDEDEDMPFTDQDDAISAHDEAFDGEN 301
           LDPNP TR+T   + +DEWFK DY P  P +++ D   D    D D      +E    E 
Sbjct: 260 LDPNPMTRVTPQEVFEDEWFKKDYKP--PVFEERD---DSNMDDIDAVFKDSEEHLVTEK 319

Query: 302 SPESPSIINAFELIGMSSSLDLSGFFEQEDVSERKTRFTSNHSAKDLLERIEIIATDMGF 361
             E P+ INAFE+I MS  L+L   F+ E   +R+TR T    A +++E+IE  A  +GF
Sbjct: 320 REEQPAAINAFEIISMSRGLNLENLFDPEQEFKRETRITLRGGANEIIEKIEEAAKPLGF 379

Query: 362 RVQKKSGKLKVMQEIRNQRSLSNLSFKAEVFEISPLLNVVELKKSFGDSSAYRQLCERLS 421
            VQKK+ K++ ++ ++  R   NL+   E+F+++P L++V++ KS GD+  + +  ++LS
Sbjct: 380 DVQKKNYKMR-LENVKAGRK-GNLNVATEIFQVAPSLHMVQVSKSKGDTLEFHKFYKKLS 439

Query: 422 NDL----GTNPELERHNS 436
           N L     TN E+++  +
Sbjct: 440 NSLEQVVWTNNEVKKETA 450

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029041.11.8e-253100.00CBL-interacting serine/threonine-protein kinase 1 [Cucurbita argyrosperma subsp.... [more]
XP_022972049.11.5e-25299.55CBL-interacting serine/threonine-protein kinase 1-like isoform X1 [Cucurbita max... [more]
KAG6597598.15.8e-25299.55CBL-interacting serine/threonine-protein kinase 1, partial [Cucurbita argyrosper... [more]
XP_023540923.11.7e-25199.10CBL-interacting serine/threonine-protein kinase 1-like [Cucurbita pepo subsp. pe... [more]
XP_022935693.14.9e-25199.55CBL-interacting serine/threonine-protein kinase 1-like isoform X1 [Cucurbita mos... [more]
Match NameE-valueIdentityDescription
Q8RWC91.8e-16669.91CBL-interacting serine/threonine-protein kinase 1 OS=Arabidopsis thaliana OX=370... [more]
Q94C403.2e-16066.13CBL-interacting serine/threonine-protein kinase 17 OS=Arabidopsis thaliana OX=37... [more]
Q9LGV51.0e-15062.28CBL-interacting protein kinase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q0D4B23.3e-14963.81CBL-interacting protein kinase 21 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q75L421.8e-14762.41CBL-interacting protein kinase 17 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
A0A6J1IAC07.4e-25399.55Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A6J1FB812.4e-25199.55Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IRR44.9e-22890.54Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3AXS94.9e-22890.32Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A0A0L9931.6e-22690.09Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_3... [more]
Match NameE-valueIdentityDescription
AT3G17510.11.2e-16769.91CBL-interacting protein kinase 1 [more]
AT1G48260.12.3e-16166.13CBL-interacting protein kinase 17 [more]
AT3G17510.23.5e-13869.23CBL-interacting protein kinase 1 [more]
AT2G26980.31.8e-12150.46CBL-interacting protein kinase 3 [more]
AT2G26980.41.8e-12150.46CBL-interacting protein kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 6..261
e-value: 1.6E-104
score: 363.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 6..261
e-value: 1.5E-72
score: 244.1
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 6..261
score: 49.968651
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 2..307
e-value: 1.3E-101
score: 342.0
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 308..426
e-value: 3.9E-36
score: 125.9
NoneNo IPR availablePIRSRPIRSR037014-2PIRSR037014-2coord: 1..270
e-value: 1.1E-77
score: 260.1
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 5..252
e-value: 6.0E-14
score: 49.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 424..444
NoneNo IPR availablePANTHERPTHR43895:SF67CBL-INTERACTING PROTEIN KINASE 21coord: 1..430
NoneNo IPR availablePANTHERPTHR43895FAMILY NOT NAMEDcoord: 1..430
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 310..421
e-value: 6.63304E-40
score: 137.318
IPR004041NAF domainPFAMPF03822NAFcoord: 306..365
e-value: 4.1E-20
score: 71.7
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 12..35
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 125..137
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 304..328
score: 13.009231
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 3..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25661-RACarg25661-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006468 protein phosphorylation
biological_process GO:0007165 signal transduction
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity