Carg25631 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25631
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionDynein assembly factor 1
LocationCarg_Chr01: 11267398 .. 11269406 (+)
RNA-Seq ExpressionCarg25631
SyntenyCarg25631
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAAATTCAATTGCTTCTCCGTTCGATCAGGGAAGAAGAAGAAAACCAAGGTATGGCTTCTTTCGCTATTCATATCTTAGTTGCTTGTCAAAAAAAATTCTGGGGGTTTGCTCAATGTATATATACACTTTACTCTATGGTTGCAGGATGGTTTAGAATCGCAAATGCCTGCTGAATTGAATAGTTTGACGAAGACCTTGCAAGTCAGCATCCATCACACTGAGGAGTCTTTTCAAGATGGAGAAGCGAAGTCATCGACCATGGACGTCGCAATTCTGTACCCGTCTGAGAATAATTCTAAGCTGGATGTTAAGGTAACGAACAATGAGAATCCTGTAGGAGGAGGAGGAGCAGTAGAAGCTGCATATGAAGGGGAAGACGAGCGTGATGACAACTCCATCAAGAGGAACCTTTCTGACTTTGATCTCGCTGCTCAAGACACCTGTGGAGAGGAATTTGAGTTTCGGTTTTCTACTTCGTTGGAAAAACAATTTGATAATACCGTCGTTGAAGGTGGAGAAGAGGCTGATGCGATCCAAACTGGACATGTTAGTGATCCTGGAATTGGTAAGGCAGTATGTTGGGCATCGCCTAAACTTAAACGCTCCTGCTCTAATCTGGAAACCAGGGATGTGCTTAGAGATCTATCTCATCCGATGCCCCCTTCAAAGTCTCAGTCTTTTGAGAAACTGCAAGAATTGGCTGACAAAATGTGGAACTATGTCGAGCCAGGCAGTCCAGAATCTATTATGACCCATCGCAGTGCTGATAAAGTGATGTTGAAGAAGCGTTCTTCGAGCCAGATTCTGCCTTCCAGAAGTAGAAGGTTGTGGTGGAAGTTGTTCCTGTGGACATCTTCCCTAAGAAGAGTGGATGAGTGGGTGAAGGATCTTCAAATTGAGCCATGTCTTTCGATCGACAACATTAGAGATGACAACGATGAAGATACTTTCTCCCCGCCATATCCCGAGAGAACTGCAAGTCACACAGCTCGACGTGGAGAACTCAATCTTACAGAAGAGATTTTGCACGCAAATAGCGTCATCCAATCTCTGAATTCTTCCTCGACCGTGGCTCACATCTCTGGTATTGGCTTAAAAGCCATACCCACAATCTCACACCTATCCGGTCTTAGATCCATCAATTTGTCCGGCAACCTCATAGGTAAGAATTCGTTTTTGTTCAAGTTATATTCTGGTTACTTGTCTTTTCCTTAATTGTTCTTGCCACTACTAACATGGCTGCTTGGTTTCCAGTTCACATCAACTCTGGATCACTGCCCAAGGGGCTGCACACTCTTAACTTGTCCAGGAACAAGATCAGCGCCATTGAAGGACTCAAAGAATTAACACGTCTTCGAATCCTCGACCTGAGTTACAATCGCATTTCTCGCATAGGACATGGTACTGTTTTGAAAACTTAGTGTAGAATTGTGTCCATTTCTGGTTATTATTCTGAGTGTGCAATTTGTTCAACTGCCTCATCAGGGCTGTCAAATTGTGCGATTATCAAGGAACTTCATCTCGCTGGCAACAAGATTAGTGATGTCGAGGGACTACATCGACTCTTGAAGCTCACTGTACTCGACCTGAGCTTCAACAAGATTTCAACAACCAAAGCTCTGGGCCAACTTGTAGCCAACTATAACTCTCTTCAAGCTCTTAATCTGTTGGGAAATCCAATTCAAAGCAACGTGAGTGATGAGCAGCTTCGCAAGGCCGTTATTGGCCTTCTTCCAAACCTTGTTTATCTAAACAAGCAGGCCATCAAAGCACAGAGAGCTCGAGAAGCAGCGACCGATAGCATTGCTAAAGCAGCACTGGGAGGGAACAGTAGTTGGAATTCTCGCAGAAGATCATCAAGGAAAACAAGCCAGATGATTGCATCGTCTTCAATTAGTGGGCGGAGGAGCAGCACTGCAAGTGTTGCTGCACACAAAGGCAGGCACAGATCAAAAGCGCCAACCCCGCGCCCTTCTTCTCTGGGGTTGGCTTCATCTCGTTAA

mRNA sequence

ATGGCAAAATTCAATTGCTTCTCCGTTCGATCAGGGAAGAAGAAGAAAACCAAGGATGGTTTAGAATCGCAAATGCCTGCTGAATTGAATAGTTTGACGAAGACCTTGCAAGTCAGCATCCATCACACTGAGGAGTCTTTTCAAGATGGAGAAGCGAAGTCATCGACCATGGACGTCGCAATTCTGTACCCGTCTGAGAATAATTCTAAGCTGGATGTTAAGGTAACGAACAATGAGAATCCTGTAGGAGGAGGAGGAGCAGTAGAAGCTGCATATGAAGGGGAAGACGAGCGTGATGACAACTCCATCAAGAGGAACCTTTCTGACTTTGATCTCGCTGCTCAAGACACCTGTGGAGAGGAATTTGAGTTTCGGTTTTCTACTTCGTTGGAAAAACAATTTGATAATACCGTCGTTGAAGGTGGAGAAGAGGCTGATGCGATCCAAACTGGACATGTTAGTGATCCTGGAATTGGTAAGGCAGTATGTTGGGCATCGCCTAAACTTAAACGCTCCTGCTCTAATCTGGAAACCAGGGATGTGCTTAGAGATCTATCTCATCCGATGCCCCCTTCAAAGTCTCAGTCTTTTGAGAAACTGCAAGAATTGGCTGACAAAATGTGGAACTATGTCGAGCCAGGCAGTCCAGAATCTATTATGACCCATCGCAGTGCTGATAAAGTGATGTTGAAGAAGCGTTCTTCGAGCCAGATTCTGCCTTCCAGAAGTAGAAGGTTGTGGTGGAAGTTGTTCCTGTGGACATCTTCCCTAAGAAGAGTGGATGAGTGGGTGAAGGATCTTCAAATTGAGCCATGTCTTTCGATCGACAACATTAGAGATGACAACGATGAAGATACTTTCTCCCCGCCATATCCCGAGAGAACTGCAAGTCACACAGCTCGACGTGGAGAACTCAATCTTACAGAAGAGATTTTGCACGCAAATAGCGTCATCCAATCTCTGAATTCTTCCTCGACCGTGGCTCACATCTCTGGTATTGGCTTAAAAGCCATACCCACAATCTCACACCTATCCGGTCTTAGATCCATCAATTTGTCCGGCAACCTCATAGTTCACATCAACTCTGGATCACTGCCCAAGGGGCTGCACACTCTTAACTTGTCCAGGAACAAGATCAGCGCCATTGAAGGACTCAAAGAATTAACACGTCTTCGAATCCTCGACCTGAGTTACAATCGCATTTCTCGCATAGGACATGGGCTGTCAAATTGTGCGATTATCAAGGAACTTCATCTCGCTGGCAACAAGATTAGTGATGTCGAGGGACTACATCGACTCTTGAAGCTCACTGTACTCGACCTGAGCTTCAACAAGATTTCAACAACCAAAGCTCTGGGCCAACTTGTAGCCAACTATAACTCTCTTCAAGCTCTTAATCTGTTGGGAAATCCAATTCAAAGCAACGTGAGTGATGAGCAGCTTCGCAAGGCCGTTATTGGCCTTCTTCCAAACCTTGTTTATCTAAACAAGCAGGCCATCAAAGCACAGAGAGCTCGAGAAGCAGCGACCGATAGCATTGCTAAAGCAGCACTGGGAGGGAACAGTAGTTGGAATTCTCGCAGAAGATCATCAAGGAAAACAAGCCAGATGATTGCATCGTCTTCAATTAGTGGGCGGAGGAGCAGCACTGCAAGTGTTGCTGCACACAAAGGCAGGCACAGATCAAAAGCGCCAACCCCGCGCCCTTCTTCTCTGGGGTTGGCTTCATCTCGTTAA

Coding sequence (CDS)

ATGGCAAAATTCAATTGCTTCTCCGTTCGATCAGGGAAGAAGAAGAAAACCAAGGATGGTTTAGAATCGCAAATGCCTGCTGAATTGAATAGTTTGACGAAGACCTTGCAAGTCAGCATCCATCACACTGAGGAGTCTTTTCAAGATGGAGAAGCGAAGTCATCGACCATGGACGTCGCAATTCTGTACCCGTCTGAGAATAATTCTAAGCTGGATGTTAAGGTAACGAACAATGAGAATCCTGTAGGAGGAGGAGGAGCAGTAGAAGCTGCATATGAAGGGGAAGACGAGCGTGATGACAACTCCATCAAGAGGAACCTTTCTGACTTTGATCTCGCTGCTCAAGACACCTGTGGAGAGGAATTTGAGTTTCGGTTTTCTACTTCGTTGGAAAAACAATTTGATAATACCGTCGTTGAAGGTGGAGAAGAGGCTGATGCGATCCAAACTGGACATGTTAGTGATCCTGGAATTGGTAAGGCAGTATGTTGGGCATCGCCTAAACTTAAACGCTCCTGCTCTAATCTGGAAACCAGGGATGTGCTTAGAGATCTATCTCATCCGATGCCCCCTTCAAAGTCTCAGTCTTTTGAGAAACTGCAAGAATTGGCTGACAAAATGTGGAACTATGTCGAGCCAGGCAGTCCAGAATCTATTATGACCCATCGCAGTGCTGATAAAGTGATGTTGAAGAAGCGTTCTTCGAGCCAGATTCTGCCTTCCAGAAGTAGAAGGTTGTGGTGGAAGTTGTTCCTGTGGACATCTTCCCTAAGAAGAGTGGATGAGTGGGTGAAGGATCTTCAAATTGAGCCATGTCTTTCGATCGACAACATTAGAGATGACAACGATGAAGATACTTTCTCCCCGCCATATCCCGAGAGAACTGCAAGTCACACAGCTCGACGTGGAGAACTCAATCTTACAGAAGAGATTTTGCACGCAAATAGCGTCATCCAATCTCTGAATTCTTCCTCGACCGTGGCTCACATCTCTGGTATTGGCTTAAAAGCCATACCCACAATCTCACACCTATCCGGTCTTAGATCCATCAATTTGTCCGGCAACCTCATAGTTCACATCAACTCTGGATCACTGCCCAAGGGGCTGCACACTCTTAACTTGTCCAGGAACAAGATCAGCGCCATTGAAGGACTCAAAGAATTAACACGTCTTCGAATCCTCGACCTGAGTTACAATCGCATTTCTCGCATAGGACATGGGCTGTCAAATTGTGCGATTATCAAGGAACTTCATCTCGCTGGCAACAAGATTAGTGATGTCGAGGGACTACATCGACTCTTGAAGCTCACTGTACTCGACCTGAGCTTCAACAAGATTTCAACAACCAAAGCTCTGGGCCAACTTGTAGCCAACTATAACTCTCTTCAAGCTCTTAATCTGTTGGGAAATCCAATTCAAAGCAACGTGAGTGATGAGCAGCTTCGCAAGGCCGTTATTGGCCTTCTTCCAAACCTTGTTTATCTAAACAAGCAGGCCATCAAAGCACAGAGAGCTCGAGAAGCAGCGACCGATAGCATTGCTAAAGCAGCACTGGGAGGGAACAGTAGTTGGAATTCTCGCAGAAGATCATCAAGGAAAACAAGCCAGATGATTGCATCGTCTTCAATTAGTGGGCGGAGGAGCAGCACTGCAAGTGTTGCTGCACACAAAGGCAGGCACAGATCAAAAGCGCCAACCCCGCGCCCTTCTTCTCTGGGGTTGGCTTCATCTCGTTAA

Protein sequence

MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVAILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGEEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILPSRSRRLWWKLFLWTSSLRRVDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASVAAHKGRHRSKAPTPRPSSLGLASSR
Homology
BLAST of Carg25631 vs. NCBI nr
Match: KAG7037598.1 (Dynein assembly factor 1, axonemal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1105.9 bits (2859), Expect = 0.0e+00
Identity = 578/578 (100.00%), Postives = 578/578 (100.00%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE
Sbjct: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120

Query: 121 EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180
           EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD
Sbjct: 121 EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180

Query: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240
           VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP
Sbjct: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240

Query: 241 SRSRRLWWKLFLWTSSLRRVDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTA 300
           SRSRRLWWKLFLWTSSLRRVDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTA
Sbjct: 241 SRSRRLWWKLFLWTSSLRRVDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTA 300

Query: 301 RRGELNLTEEILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHI 360
           RRGELNLTEEILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHI
Sbjct: 301 RRGELNLTEEILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHI 360

Query: 361 NSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLA 420
           NSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLA
Sbjct: 361 NSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLA 420

Query: 421 GNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQ 480
           GNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQ
Sbjct: 421 GNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQ 480

Query: 481 LRKAVIGLLPNLVYLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIAS 540
           LRKAVIGLLPNLVYLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIAS
Sbjct: 481 LRKAVIGLLPNLVYLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIAS 540

Query: 541 SSISGRRSSTASVAAHKGRHRSKAPTPRPSSLGLASSR 579
           SSISGRRSSTASVAAHKGRHRSKAPTPRPSSLGLASSR
Sbjct: 541 SSISGRRSSTASVAAHKGRHRSKAPTPRPSSLGLASSR 578

BLAST of Carg25631 vs. NCBI nr
Match: KAG6608246.1 (Dynein assembly factor 1, axonemal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1057.0 bits (2732), Expect = 5.9e-305
Identity = 576/672 (85.71%), Postives = 577/672 (85.86%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPV----GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQD 120
           ILYPSENNSKLDVKVTNNENPV    GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQD
Sbjct: 61  ILYPSENNSKLDVKVTNNENPVGGGGGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQD 120

Query: 121 TCGEEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNL 180
           TCGEEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNL
Sbjct: 121 TCGEEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNL 180

Query: 181 ETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSS 240
           ETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSS
Sbjct: 181 ETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSS 240

Query: 241 QILPSRSRRLWWKLFLW------------------------------------------- 300
           QILPSRSRRLWWKLFLW                                           
Sbjct: 241 QILPSRSRRLWWKLFLWSHRNLQKPWTTKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMM 300

Query: 301 -----------------------------------------------TSSLRRVDEWVKD 360
                                                          +SSLRRVDEWVKD
Sbjct: 301 ESPGSFAEETWTNDPNNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKD 360

Query: 361 LQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSST 420
           LQIEPCLSIDNIRDDNDE TFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSST
Sbjct: 361 LQIEPCLSIDNIRDDNDEGTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSST 420

Query: 421 VAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLK 480
           VAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLK
Sbjct: 421 VAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLK 480

Query: 481 ELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKI 540
           ELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKI
Sbjct: 481 ELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKI 540

Query: 541 STTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAR 579
           STTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAR
Sbjct: 541 STTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAR 600

BLAST of Carg25631 vs. NCBI nr
Match: XP_022941111.1 (uncharacterized protein LOC111446507 [Cucurbita moschata])

HSP 1 Score: 1050.4 bits (2715), Expect = 5.5e-303
Identity = 574/668 (85.93%), Postives = 575/668 (86.08%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVKVTNNENPV GGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE
Sbjct: 61  ILYPSENNSKLDVKVTNNENPV-GGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120

Query: 121 EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180
           EFEFRFSTSLEKQFDNT  EGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD
Sbjct: 121 EFEFRFSTSLEKQFDNT--EGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180

Query: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240
           VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP
Sbjct: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240

Query: 241 SRSRRLWWKLFLW----------------------------------------------- 300
           SRSRRLWWKLFLW                                               
Sbjct: 241 SRSRRLWWKLFLWSHRNLQKPWTTKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMMESPG 300

Query: 301 -------------------------------------------TSSLRRVDEWVKDLQIE 360
                                                      +SSLRRVDEWVKDLQIE
Sbjct: 301 SFAEETWTNDPNNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQIE 360

Query: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420
           PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI
Sbjct: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420

Query: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480
           SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR
Sbjct: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480

Query: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540
           LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK
Sbjct: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540

Query: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 579
           ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT
Sbjct: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 600

BLAST of Carg25631 vs. NCBI nr
Match: XP_022981325.1 (uncharacterized protein LOC111480488 [Cucurbita maxima])

HSP 1 Score: 1040.8 bits (2690), Expect = 4.4e-300
Identity = 571/670 (85.22%), Postives = 573/670 (85.52%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPV-GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCG 120
           ILYPSENNSKLDVKVTNNENPV GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDT G
Sbjct: 61  ILYPSENNSKLDVKVTNNENPVGGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTSG 120

Query: 121 EEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR 180
           EEFEFRFSTSLEKQFDNTVVEGGE ADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR
Sbjct: 121 EEFEFRFSTSLEKQFDNTVVEGGEGADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR 180

Query: 181 DVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL 240
           DVLRDLSH MPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL
Sbjct: 181 DVLRDLSHQMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL 240

Query: 241 PSRSRRLWWKLFLW---------------------------------------------- 300
           PSRSRRLWWKLFLW                                              
Sbjct: 241 PSRSRRLWWKLFLWSHRNLQKPWTAKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMMESP 300

Query: 301 --------------------------------------------TSSLRRVDEWVKDLQI 360
                                                       +SSLRRVDEWVKDLQI
Sbjct: 301 GSYAEETWTNDPNNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQI 360

Query: 361 EPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH 420
           EPCLSIDNIRDDNDEDTFS PYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH
Sbjct: 361 EPCLSIDNIRDDNDEDTFSLPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH 420

Query: 421 ISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT 480
           ISGIGLKAIPTISHLSGLRS+NLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT
Sbjct: 421 ISGIGLKAIPTISHLSGLRSVNLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT 480

Query: 481 RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT 540
           RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT
Sbjct: 481 RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT 540

Query: 541 KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAA 579
           KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREA 
Sbjct: 541 KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAV 600

BLAST of Carg25631 vs. NCBI nr
Match: XP_023524108.1 (uncharacterized protein LOC111788125 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1039.3 bits (2686), Expect = 1.3e-299
Identity = 566/668 (84.73%), Postives = 570/668 (85.33%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKK KDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKAKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVK TNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE
Sbjct: 61  ILYPSENNSKLDVKETNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120

Query: 121 EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180
           EFEFRFSTSLEKQFDNTVVEGGE ADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD
Sbjct: 121 EFEFRFSTSLEKQFDNTVVEGGEGADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180

Query: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240
           VLRDLSH MPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP
Sbjct: 181 VLRDLSHQMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240

Query: 241 SRSRRLWWKLFLW----------------------------------------------- 300
           SRSRRLWWKLFLW                                               
Sbjct: 241 SRSRRLWWKLFLWSHRNLQKPWTTKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMMESPG 300

Query: 301 -------------------------------------------TSSLRRVDEWVKDLQIE 360
                                                      +SSLRRVDEWVKDLQIE
Sbjct: 301 SFAEETWTNDPSNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQIE 360

Query: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420
           P LSID IRDDNDEDTFSPP PERTASHTARRGE NLTEEILHANSVIQSLNSSSTVAHI
Sbjct: 361 PRLSIDYIRDDNDEDTFSPPCPERTASHTARRGEPNLTEEILHANSVIQSLNSSSTVAHI 420

Query: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480
           SGIGLKAIPTISHLSGLRS+NLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR
Sbjct: 421 SGIGLKAIPTISHLSGLRSVNLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480

Query: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540
           LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK
Sbjct: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540

Query: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 579
           ALGQLVANYNSLQALNLLGNPIQSN+SDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT
Sbjct: 541 ALGQLVANYNSLQALNLLGNPIQSNMSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 600

BLAST of Carg25631 vs. ExPASy Swiss-Prot
Match: Q54Q39 (Protein phosphatase 1 regulatory subunit pprA OS=Dictyostelium discoideum OX=44689 GN=pprA PE=3 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 1.0e-12
Identity = 59/185 (31.89%), Postives = 92/185 (49.73%), Query Frame = 0

Query: 338 IPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLS 397
           I  +  L  ++++ L  N +  I +      + TL L RNKI+ I+G+  L+ LRIL L 
Sbjct: 153 IENLQELVPIKNLELGSNRLREIENLENLVNIETLWLGRNKITEIKGINHLSHLRILSLQ 212

Query: 398 YNRISRIG-HGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLV 457
            NR++ IG  GL     ++EL+L+ N I+D++GL  L +L  LD+S NKI T   L +L 
Sbjct: 213 SNRLTEIGVKGLVGLNCLEELYLSHNGITDIDGLQSLKQLRTLDISANKIKTLVGLNELP 272

Query: 458 ------ANYN--------------SLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLN 502
                  N N              S++ L    NP+ ++V   Q R+  I + P L  L+
Sbjct: 273 DLDEIWCNDNLVDSMDNIEQQVTKSIKCLYFERNPVATHV---QYRRMFINMFPQLKQLD 332

BLAST of Carg25631 vs. ExPASy Swiss-Prot
Match: P36047 (Protein phosphatase 1 regulatory subunit SDS22 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=SDS22 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.5e-12
Identity = 56/159 (35.22%), Postives = 85/159 (53.46%), Query Frame = 0

Query: 344 LSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISR 403
           LS L  I L  N I  + +    K L  L++  NK+  IE L+ELT L  L LS+N I++
Sbjct: 180 LSNLEEIWLGKNSIPRLINLHPLKNLKILSIQSNKLKKIENLEELTNLEELYLSHNFITK 239

Query: 404 IGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT-KALGQLVANYNSL 463
           I  GL     +  L +  NKI+ +E L+ L  LT +  SFNKI  + ++LG+ ++  + L
Sbjct: 240 I-EGLEKNLKLTTLDVTSNKITSLENLNHLSNLTDIWASFNKIDQSFESLGENLSALSRL 299

Query: 464 QALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIK 502
           + + L GNPIQ        RK  + L P+L  ++   I+
Sbjct: 300 ETIYLEGNPIQLENKTSYRRKLTMNLPPSLQKIDATYIR 337

BLAST of Carg25631 vs. ExPASy Swiss-Prot
Match: Q9D2H9 (Dynein axonemal assembly factor 1 OS=Mus musculus OX=10090 GN=Dnaaf1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 1.8e-09
Identity = 72/245 (29.39%), Postives = 114/245 (46.53%), Query Frame = 0

Query: 334 GLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRI 393
           G   I  +   +GLR + L  N I  I +      L  L L  N +  IE L+ L +L  
Sbjct: 112 GFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDA 171

Query: 394 LDLSYNRISRIGHGLSNCAIIKELHLAGNK---ISDVEGLHRLLKLTVLDLSFNKISTTK 453
           L+LS N I  I   LS   ++  L +A N+   ++D+E L   L+L VLDLS N +S  +
Sbjct: 172 LNLSNNYIKTI-ENLSCLPVLNTLQMAHNRLETVADIEHLRECLRLCVLDLSHNALSDPE 231

Query: 454 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAI--KAQRAREA 513
            L  ++ +   L+ LNL+GNP+  ++ +   R+ V   L +L YL+ + +  K +   EA
Sbjct: 232 IL-SVLESMPCLRVLNLMGNPVTKHIPN--YRRTVTVRLKHLTYLDDRPVFPKDRACAEA 291

Query: 514 ATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASVAAHKGRHRSKAPTPR 573
                  A       W SR    +K +  + + ++  RR      A  + + R +  TP 
Sbjct: 292 WARGGYAAEKEERRQWESREH--KKITDSLEALAMIKRR------AEERKKARDRGETPL 344

BLAST of Carg25631 vs. ExPASy Swiss-Prot
Match: Q6AYH9 (Dynein axonemal assembly factor 1 OS=Rattus norvegicus OX=10116 GN=Dnaaf1 PE=1 SV=1)

HSP 1 Score: 64.3 bits (155), Expect = 5.1e-09
Identity = 71/243 (29.22%), Postives = 112/243 (46.09%), Query Frame = 0

Query: 334 GLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRI 393
           G   I  +   +GLR + L  N I  I +      L  L L  N +  IE L+ L +L  
Sbjct: 112 GFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDA 171

Query: 394 LDLSYNRISRIGHGLSNCAIIKELHLAGNK---ISDVEGLHRLLKLTVLDLSFNKISTTK 453
           L+LS N I  I   LS   ++  L +A N+   ++D+E L   L+L VLDLS N +S  +
Sbjct: 172 LNLSNNYIKTI-ENLSCLPVLNTLQMAHNRLETVADIEHLRECLQLCVLDLSHNSLSDPE 231

Query: 454 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAI--KAQRAREA 513
            L  ++     L+ LNL+GNP+  ++ +   R+ V   L +L YL+ + +  K +   EA
Sbjct: 232 IL-SVLETMPCLRVLNLMGNPVTKHIPN--YRRTVTVRLKHLTYLDDRPVFPKDRACAEA 291

Query: 514 ATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASVAAHKGRHRSKAPTPR 572
                  A       W SR    +K +  + + ++  RR      A  + + R +  TP 
Sbjct: 292 WARGGYAAEKEERHQWESREH--KKITDSLEALAMIKRR------AEERKKARDRGETPL 342

BLAST of Carg25631 vs. ExPASy Swiss-Prot
Match: B6D5P3 (Dynein axonemal assembly factor 1 OS=Peromyscus leucopus OX=10041 GN=Dnaaf1 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 6.7e-09
Identity = 71/243 (29.22%), Postives = 112/243 (46.09%), Query Frame = 0

Query: 334 GLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTRLRI 393
           G   I  +   +GLR + L  N I  I +      L  L L  N +  IE L+ L +L  
Sbjct: 112 GFDRIENLEEYTGLRCLWLECNGIQRIENLQAQSELRCLFLQVNLLHKIENLEPLQKLDA 171

Query: 394 LDLSYNRISRIGHGLSNCAIIKELHLAGNK---ISDVEGLHRLLKLTVLDLSFNKISTTK 453
           L+LS N I  I   LS   ++  L +A N+   ++D++ L   L+L VLDLS N +S  +
Sbjct: 172 LNLSNNYIKTI-ENLSCLPVLNTLQMAHNRLETVADIQHLRECLRLCVLDLSHNMLSDPE 231

Query: 454 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAI--KAQRAREA 513
            L  ++ +   L+ LNL+GNP+  ++ +   R+ V   L  L YL+ + +  K +   EA
Sbjct: 232 IL-SVLESMPCLRVLNLMGNPVTKHIPN--YRRTVTVRLKQLTYLDDRPVFPKDRACAEA 291

Query: 514 ATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASVAAHKGRHRSKAPTPR 572
                  A       W SR    +K +  + + ++  RR      A  + + R K  TP 
Sbjct: 292 WARGGYAAEKEERLQWESREH--KKITDSLEALAMIKRR------AEERKKARDKGETPL 342

BLAST of Carg25631 vs. ExPASy TrEMBL
Match: A0A6J1FK88 (uncharacterized protein LOC111446507 OS=Cucurbita moschata OX=3662 GN=LOC111446507 PE=4 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.7e-303
Identity = 574/668 (85.93%), Postives = 575/668 (86.08%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVKVTNNENPV GGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE
Sbjct: 61  ILYPSENNSKLDVKVTNNENPV-GGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120

Query: 121 EFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180
           EFEFRFSTSLEKQFDNT  EGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD
Sbjct: 121 EFEFRFSTSLEKQFDNT--EGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETRD 180

Query: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240
           VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP
Sbjct: 181 VLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILP 240

Query: 241 SRSRRLWWKLFLW----------------------------------------------- 300
           SRSRRLWWKLFLW                                               
Sbjct: 241 SRSRRLWWKLFLWSHRNLQKPWTTKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMMESPG 300

Query: 301 -------------------------------------------TSSLRRVDEWVKDLQIE 360
                                                      +SSLRRVDEWVKDLQIE
Sbjct: 301 SFAEETWTNDPNNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQIE 360

Query: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420
           PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI
Sbjct: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420

Query: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480
           SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR
Sbjct: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480

Query: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540
           LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK
Sbjct: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540

Query: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 579
           ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT
Sbjct: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 600

BLAST of Carg25631 vs. ExPASy TrEMBL
Match: A0A6J1IZ63 (uncharacterized protein LOC111480488 OS=Cucurbita maxima OX=3661 GN=LOC111480488 PE=4 SV=1)

HSP 1 Score: 1040.8 bits (2690), Expect = 2.1e-300
Identity = 571/670 (85.22%), Postives = 573/670 (85.52%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA
Sbjct: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPV-GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCG 120
           ILYPSENNSKLDVKVTNNENPV GGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDT G
Sbjct: 61  ILYPSENNSKLDVKVTNNENPVGGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTSG 120

Query: 121 EEFEFRFSTSLEKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR 180
           EEFEFRFSTSLEKQFDNTVVEGGE ADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR
Sbjct: 121 EEFEFRFSTSLEKQFDNTVVEGGEGADAIQTGHVSDPGIGKAVCWASPKLKRSCSNLETR 180

Query: 181 DVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL 240
           DVLRDLSH MPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL
Sbjct: 181 DVLRDLSHQMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQIL 240

Query: 241 PSRSRRLWWKLFLW---------------------------------------------- 300
           PSRSRRLWWKLFLW                                              
Sbjct: 241 PSRSRRLWWKLFLWSHRNLQKPWTAKAAAPTSSAFNQQGGYCSDNLEPNRAVGKSMMESP 300

Query: 301 --------------------------------------------TSSLRRVDEWVKDLQI 360
                                                       +SSLRRVDEWVKDLQI
Sbjct: 301 GSYAEETWTNDPNNSKVEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQI 360

Query: 361 EPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH 420
           EPCLSIDNIRDDNDEDTFS PYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH
Sbjct: 361 EPCLSIDNIRDDNDEDTFSLPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAH 420

Query: 421 ISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT 480
           ISGIGLKAIPTISHLSGLRS+NLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT
Sbjct: 421 ISGIGLKAIPTISHLSGLRSVNLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELT 480

Query: 481 RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT 540
           RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT
Sbjct: 481 RLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTT 540

Query: 541 KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAA 579
           KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREA 
Sbjct: 541 KALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAV 600

BLAST of Carg25631 vs. ExPASy TrEMBL
Match: A0A1S3CQZ9 (uncharacterized protein LOC103503704 OS=Cucumis melo OX=3656 GN=LOC103503704 PE=4 SV=1)

HSP 1 Score: 847.8 bits (2189), Expect = 2.6e-242
Identity = 488/678 (71.98%), Postives = 517/678 (76.25%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFS R+GKKKKTK  LESQ PAELN L KTLQVSIHH+EE FQ GE KSST+DVA
Sbjct: 1   MAKFNCFSGRTGKKKKTKTDLESQKPAELNYLKKTLQVSIHHSEEPFQVGEGKSSTLDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVKVTN+E+P    GAVE  YEGEDERDDNSIKRNLSDFDL AQD CG 
Sbjct: 61  ILYPSENNSKLDVKVTNHESP--AVGAVETEYEGEDERDDNSIKRNLSDFDLPAQDNCGG 120

Query: 121 EFEFRFSTSLEKQFDNTVVEG---------GEEADA-IQTGHVSDPGIGKAVCWASPKLK 180
           EFEF+    L   FD    EG         GE  DA IQ GH+SDPGIGKAVCWASPKLK
Sbjct: 121 EFEFQL---LGNHFDKITTEGEGGEGIGVEGEGVDASIQNGHLSDPGIGKAVCWASPKLK 180

Query: 181 RSCSNLETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVML 240
           RSCSNLETRDVLRDLSH +PP KSQSFEKLQELA++M +YV+PGSP SIMTHRSADKVML
Sbjct: 181 RSCSNLETRDVLRDLSHQLPPPKSQSFEKLQELANEMRDYVDPGSPGSIMTHRSADKVML 240

Query: 241 KKRSSSQILPSRSRRLWWKLFLW------------------------------------- 300
           KKRSSSQILPSRSRRLWWKLFLW                                     
Sbjct: 241 KKRSSSQILPSRSRRLWWKLFLWSHRNLQKPWTIKATPTNSAFNQQGGYCSDNLEPNRAA 300

Query: 301 ------------------------------------------------------TSSLRR 360
                                                                 +SSLRR
Sbjct: 301 GKSMLESPESFTEESWTNGPCNNKSDDQDQVQENLCNGVSGLWPQNQWVAFSAESSSLRR 360

Query: 361 VDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQ 420
           VDEWVKDLQIEPCLSID +  DNDE T  PP PERT++HT RRGE NLTEEIL+ANSVIQ
Sbjct: 361 VDEWVKDLQIEPCLSIDEVGGDNDEATVFPPSPERTSTHTPRRGEPNLTEEILYANSVIQ 420

Query: 421 SLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKI 480
           SLNSSSTVAHISGIGL+AIPTISHLSGLRS+NLSGNLI+HIN GSLPKGLHTLNLSRNKI
Sbjct: 421 SLNSSSTVAHISGIGLRAIPTISHLSGLRSVNLSGNLILHINPGSLPKGLHTLNLSRNKI 480

Query: 481 SAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVL 540
           S IEGLKELTRLRILDLSYNRISRIGHGLSNC IIKEL+LAGNKISDVEGLHR+LKLTVL
Sbjct: 481 SVIEGLKELTRLRILDLSYNRISRIGHGLSNCTIIKELYLAGNKISDVEGLHRILKLTVL 540

Query: 541 DLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQA 578
           DLSFNKISTTKALGQLVANYN+LQALNLLGNPIQSNVSD+QLRKAV GLLPNLVYLNKQA
Sbjct: 541 DLSFNKISTTKALGQLVANYNTLQALNLLGNPIQSNVSDDQLRKAVTGLLPNLVYLNKQA 600

BLAST of Carg25631 vs. ExPASy TrEMBL
Match: A0A0A0LDY6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G889720 PE=4 SV=1)

HSP 1 Score: 845.9 bits (2184), Expect = 1.0e-241
Identity = 486/676 (71.89%), Postives = 518/676 (76.63%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAELNSLTKTLQVSIHHTEESFQDGEAKSSTMDVA 60
           MAKFNCFS R+GKKKKTK  LESQ PAELN L KTLQVSIHH+EE FQ GE KSST+DVA
Sbjct: 1   MAKFNCFSGRTGKKKKTKSDLESQKPAELNYLKKTLQVSIHHSEEPFQVGEGKSSTLDVA 60

Query: 61  ILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCGE 120
           ILYPSENNSKLDVKVTN+E+P   GGAVEA YEGEDERDDNSIKRN SDFDL AQD CGE
Sbjct: 61  ILYPSENNSKLDVKVTNHESPT-VGGAVEAEYEGEDERDDNSIKRNPSDFDLPAQDNCGE 120

Query: 121 EFEFRFSTSLEKQFDNTVVEG---------GEEADA-IQTGHVSDPGIGKAVCWASPKLK 180
           EFEF+    L   FD   +EG         GE  DA IQ GH+SDPGIGKAVCWASPKLK
Sbjct: 121 EFEFQL---LGNHFDKITIEGEGGEGIGVEGEGVDASIQNGHLSDPGIGKAVCWASPKLK 180

Query: 181 RSCSNLETRDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVML 240
           RSCSNLETRDVLRDLSH +PP KSQSFEKLQELA++M +YV+PGSP S MTHRSADKVML
Sbjct: 181 RSCSNLETRDVLRDLSHQLPPPKSQSFEKLQELANEMRDYVDPGSPGSTMTHRSADKVML 240

Query: 241 KKRSSSQILPSRSRRLWWKLFLW------------------------------------- 300
           KKRSSSQILPSRSRRLWWKLFLW                                     
Sbjct: 241 KKRSSSQILPSRSRRLWWKLFLWSHRNLQKPWTIKATPTSSAFNQQGGYCSDNLEPNRAA 300

Query: 301 ----------------------------------------------------TSSLRRVD 360
                                                               +SSLRRVD
Sbjct: 301 GKSMIESPGSFTEESWTNGPCNNKSDDQDQEKLCNGVSGLWPQNQWVAFSAESSSLRRVD 360

Query: 361 EWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSL 420
           EWVKDLQIEPC++ID +  DNDE T  PP PERT++HT RRGE NLTEEIL+ANSVIQSL
Sbjct: 361 EWVKDLQIEPCITIDEVGGDNDEATVFPPSPERTSTHTPRRGETNLTEEILYANSVIQSL 420

Query: 421 NSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISA 480
           NSSSTVAHISGIGL+AIPTISHLSGLRS+NLSGNLI+HIN GSLPKGLHTLNLSRNKIS 
Sbjct: 421 NSSSTVAHISGIGLRAIPTISHLSGLRSVNLSGNLILHINPGSLPKGLHTLNLSRNKISV 480

Query: 481 IEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDL 540
           IEGLKELTRLRILDLSYNRISRIGHGLSNC IIKEL+LAGNKISDVEGLHR+LKLTVLDL
Sbjct: 481 IEGLKELTRLRILDLSYNRISRIGHGLSNCTIIKELYLAGNKISDVEGLHRILKLTVLDL 540

Query: 541 SFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIK 578
           SFNKISTTK+LGQLVANYN+LQALNLLGNPIQSNVSD+QLRKAV GLLPNLVYLNKQAIK
Sbjct: 541 SFNKISTTKSLGQLVANYNTLQALNLLGNPIQSNVSDDQLRKAVTGLLPNLVYLNKQAIK 600

BLAST of Carg25631 vs. ExPASy TrEMBL
Match: A0A6J1BR66 (uncharacterized protein LOC111004961 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111004961 PE=4 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 1.4e-238
Identity = 480/668 (71.86%), Postives = 520/668 (77.84%), Query Frame = 0

Query: 1   MAKFNCFSVRSGKKKKTKDGLESQMPAEL-NSLTKTLQVSIHHTEESFQDGEAKSSTMDV 60
           MAKFNCFSVR+GKKKKTK  LES+ PAE  N+L++TLQVSIHH EE FQ  EAKSST+DV
Sbjct: 1   MAKFNCFSVRAGKKKKTKGDLESKTPAETNNNLSQTLQVSIHHPEEPFQVEEAKSSTLDV 60

Query: 61  AILYPSENNSKLDVKVTNNENPVGGGGAVEAAYEGEDERDDNSIKRNLSDFDLAAQDTCG 120
           +I YPSEN+S+LDVK+TN+E P+   GAVEAAYEGEDERDDNSIKRNLSD+DL AQ TCG
Sbjct: 61  SIHYPSENDSRLDVKITNHEIPI-RRGAVEAAYEGEDERDDNSIKRNLSDYDLPAQATCG 120

Query: 121 EEFEFRFSTSLEKQFDNTVVEGGEEAD-AIQTGHVSDPGIGKAVCWASPKLKRSCSNLET 180
           EEFEFR S +  KQFD   +EGG  AD  IQ+GHVSDPGIG+AV WASPKLKRSCSNLET
Sbjct: 121 EEFEFRLSNASGKQFDKITIEGGGGADMTIQSGHVSDPGIGRAVSWASPKLKRSCSNLET 180

Query: 181 RDVLRDLSHPMPPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQI 240
           RDVLR+L+H +PP+KSQS+E+LQ LADKM NYV+PGSP S+MTH SADKVMLKKRSSSQI
Sbjct: 181 RDVLRNLTHQLPPTKSQSYEELQGLADKMRNYVDPGSPGSVMTHHSADKVMLKKRSSSQI 240

Query: 241 LPSRSRRLWWKLFLW--------------------------------------------- 300
           LPSRSRRLWWKLFLW                                             
Sbjct: 241 LPSRSRRLWWKLFLWSHRNLQNPWSTKAPPTSFAFNQQGGYCSDSLEPNRAAGKGMMESP 300

Query: 301 -------------------------------------------TSSLRRVDEWVKDLQIE 360
                                                      +SSLRRVDEWVKDLQI+
Sbjct: 301 GSFAEETWAGPSNIKSEDQNQESLCNGVSGLWPQNQWVAFSAESSSLRRVDEWVKDLQIQ 360

Query: 361 PCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILHANSVIQSLNSSSTVAHI 420
           P L + +  DD D DTF PP PER+++H  RRGE NLTEEIL+ANSVIQSLNSSSTVAHI
Sbjct: 361 PRLEVGDDGDD-DADTFFPPSPERSSTHITRRGEPNLTEEILYANSVIQSLNSSSTVAHI 420

Query: 421 SGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLNLSRNKISAIEGLKELTR 480
           SGIGLKAIPTISHLSGLRS+NLSGNLIVHIN GSLPKGLHTLNLSRNKISAIEGLKELTR
Sbjct: 421 SGIGLKAIPTISHLSGLRSVNLSGNLIVHINPGSLPKGLHTLNLSRNKISAIEGLKELTR 480

Query: 481 LRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540
           LRILDLSYNRISRIGHGLSNCAIIKEL+LAGNKISDVEGLHRLLKLTVLDLSFNKISTTK
Sbjct: 481 LRILDLSYNRISRIGHGLSNCAIIKELYLAGNKISDVEGLHRLLKLTVLDLSFNKISTTK 540

Query: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLVYLNKQAIKAQRAREAAT 578
           ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAV+GLLPNLVYLNKQAIKAQRARE AT
Sbjct: 541 ALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVVGLLPNLVYLNKQAIKAQRAREVAT 600

BLAST of Carg25631 vs. TAIR 10
Match: AT1G78230.1 (Outer arm dynein light chain 1 protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.8e-111
Identity = 271/577 (46.97%), Postives = 335/577 (58.06%), Query Frame = 0

Query: 73  VKVTNNENPVGGGGAVE-AAYEGEDERDD-NSIKRNLSDFDLAAQDTCGEEFEFRFSTSL 132
           VK   N+ P      ++  +Y+GEDERDD +S  R  S F +        E       S 
Sbjct: 136 VKPLENDEPNNKDSPIKHESYDGEDERDDKDSTNREFSKFHV-------HERAVESVKSK 195

Query: 133 EKQFDNTVVEGGEEADAIQTGHVSDPGIGKAVCW-ASPKLKRSCSNLETRDVLRDLSHPM 192
           E+  D +  E G++ D  + GHVSDPG+GK   W  SPKLKRSCS L             
Sbjct: 196 EEVTDTS--ENGDDNDEDEGGHVSDPGLGKTTAWVTSPKLKRSCSTL------------- 255

Query: 193 PPSKSQSFEKLQELADKMWNYVEPGSPESIMTHRSADKVMLKKRSSSQILPSRSRRLWWK 252
             SK        +L D       P S ES+ +HRSAD+VMLKK SS QILPS SRRLWWK
Sbjct: 256 --SKFNGRFHGSDLHD--LKETVPVSNESVRSHRSADRVMLKKHSSMQILPSGSRRLWWK 315

Query: 253 LFLW-------------------------------------------------------- 312
           LFLW                                                        
Sbjct: 316 LFLWSHRNLHKHRVSLKSQPLNKNQQSGYTSDFAEHNQSSHEEESTNYCADFTNQSSNLW 375

Query: 313 ------------TSSLRRVDEWVKDLQIEPCLSIDNIRDDNDEDTFSPPYPERTASHTAR 372
                       +SS++RVDEWV+ L +E  + ++  +D       SP        +  +
Sbjct: 376 PHHNQWVAFSAESSSMKRVDEWVRGLDVETVVPVNEDKDVLAIFPTSPNTERSPLGNVVQ 435

Query: 373 RGELNLTEEILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHIN 432
            G  N++E I+HANS+IQSL+ SS+VAHIS IGLKAIP+ISH + L+SI+LS N IV I 
Sbjct: 436 SG--NVSEAIVHANSLIQSLSKSSSVAHISSIGLKAIPSISHFTSLKSIDLSNNFIVQIT 495

Query: 433 SGSLPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAG 492
             SLPKGLH LNLS+NKIS IEGL++LTRLR+LDLSYNRISRIG GLSNC +IKEL+LAG
Sbjct: 496 PASLPKGLHALNLSKNKISVIEGLRDLTRLRVLDLSYNRISRIGQGLSNCTLIKELYLAG 555

Query: 493 NKISDVEGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQL 552
           NKIS+VEGLHRLLKL VLDLSFNKI+TTKA+GQLVANYNSL ALN+LGNPIQ+NV ++QL
Sbjct: 556 NKISNVEGLHRLLKLIVLDLSFNKIATTKAIGQLVANYNSLVALNILGNPIQNNVGEDQL 615

Query: 553 RKAVIGLLPNLVYLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRR-RSSRKTSQMIAS 577
           RK V  LLP LVY NKQ IK QRARE   DS+A+AA GG  S + RR R+S K+    AS
Sbjct: 616 RKTVSSLLPKLVYHNKQLIKPQRAREVLKDSVARAAFGGGDSLHHRRKRTSTKSVVGSAS 675

BLAST of Carg25631 vs. TAIR 10
Match: AT4G03260.1 (Outer arm dynein light chain 1 protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.3e-68
Identity = 157/314 (50.00%), Postives = 205/314 (65.29%), Query Frame = 0

Query: 255 SSLRRVDEWVKDLQ-IEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILH 314
           S  +R+++WV DLQ +      D I   +DE    P      A+ +A+   + LT  +  
Sbjct: 328 SKFKRIEDWVNDLQHVNLSEEADEITGYDDELPREPVVLNEQATSSAKVDAIKLTPGMEA 387

Query: 315 ANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLN 374
           A   I SL++S+T A +   GL  IP +S   GLR +NLSGN IV I +G+LP+GLH LN
Sbjct: 388 AKKYISSLSASATTAQLVSHGLVVIPFLSAFVGLRVLNLSGNAIVRITAGALPRGLHALN 447

Query: 375 LSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRL 434
           LS+N IS IEGL+ELTRLR+LDLSYNRI R+GHGL++C+ +KEL+LAGNKIS++EGLHRL
Sbjct: 448 LSKNSISVIEGLRELTRLRVLDLSYNRILRLGHGLASCSSLKELYLAGNKISEIEGLHRL 507

Query: 435 LKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLV 494
           LKLTVLDL FNK STTK LGQL ANY+SLQA++L GNP Q NV DEQLRK ++GLLPNLV
Sbjct: 508 LKLTVLDLRFNKFSTTKCLGQLAANYSSLQAISLEGNPAQKNVGDEQLRKYLLGLLPNLV 567

Query: 495 YLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASV 554
           Y N+Q  K  R    +T  + +         +  + SSRK+S   +SS   G  ++  + 
Sbjct: 568 YYNRQGTKDARL-GTSTHQLDRGLR------SELKNSSRKSSHGASSSHKPGSSTARKAP 627

Query: 555 AAHK--GRHRSKAP 566
           A  K     RS+ P
Sbjct: 628 ALQKRSKERRSRLP 634

BLAST of Carg25631 vs. TAIR 10
Match: AT4G03260.2 (Outer arm dynein light chain 1 protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.3e-68
Identity = 157/314 (50.00%), Postives = 205/314 (65.29%), Query Frame = 0

Query: 255 SSLRRVDEWVKDLQ-IEPCLSIDNIRDDNDEDTFSPPYPERTASHTARRGELNLTEEILH 314
           S  +R+++WV DLQ +      D I   +DE    P      A+ +A+   + LT  +  
Sbjct: 328 SKFKRIEDWVNDLQHVNLSEEADEITGYDDELPREPVVLNEQATSSAKVDAIKLTPGMEA 387

Query: 315 ANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGNLIVHINSGSLPKGLHTLN 374
           A   I SL++S+T A +   GL  IP +S   GLR +NLSGN IV I +G+LP+GLH LN
Sbjct: 388 AKKYISSLSASATTAQLVSHGLVVIPFLSAFVGLRVLNLSGNAIVRITAGALPRGLHALN 447

Query: 375 LSRNKISAIEGLKELTRLRILDLSYNRISRIGHGLSNCAIIKELHLAGNKISDVEGLHRL 434
           LS+N IS IEGL+ELTRLR+LDLSYNRI R+GHGL++C+ +KEL+LAGNKIS++EGLHRL
Sbjct: 448 LSKNSISVIEGLRELTRLRVLDLSYNRILRLGHGLASCSSLKELYLAGNKISEIEGLHRL 507

Query: 435 LKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPIQSNVSDEQLRKAVIGLLPNLV 494
           LKLTVLDL FNK STTK LGQL ANY+SLQA++L GNP Q NV DEQLRK ++GLLPNLV
Sbjct: 508 LKLTVLDLRFNKFSTTKCLGQLAANYSSLQAISLEGNPAQKNVGDEQLRKYLLGLLPNLV 567

Query: 495 YLNKQAIKAQRAREAATDSIAKAALGGNSSWNSRRRSSRKTSQMIASSSISGRRSSTASV 554
           Y N+Q  K  R    +T  + +         +  + SSRK+S   +SS   G  ++  + 
Sbjct: 568 YYNRQGTKDARL-GTSTHQLDRGLR------SELKNSSRKSSHGASSSHKPGSSTARKAP 627

Query: 555 AAHK--GRHRSKAP 566
           A  K     RS+ P
Sbjct: 628 ALQKRSKERRSRLP 634

BLAST of Carg25631 vs. TAIR 10
Match: AT3G17920.1 (Outer arm dynein light chain 1 protein )

HSP 1 Score: 60.1 bits (144), Expect = 6.9e-09
Identity = 49/165 (29.70%), Postives = 85/165 (51.52%), Query Frame = 0

Query: 310 EILHANSVIQSLNSSSTVAHISGIGLKAIPTISHLSGLRSINLSGN-LIVHINSGSLPKG 369
           E+ H    +   NS+  + H+    +  I      + L  I+ + N L++   S  L   
Sbjct: 133 ELRHTLEKLICHNSTDALRHVFASRIAEIKDSPQWNKLAFISCACNRLVLMDESLQLLPA 192

Query: 370 LHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIGH-GLSNCAIIKELHLAGNKISDV 429
           + +L+LSRNK + ++ L+   +L+ LDL +N++ +I H    +C ++K L L  N ++ +
Sbjct: 193 VESLDLSRNKFAKVDNLRRCNKLKHLDLGFNQLRKISHLSEVSCHLVK-LVLRNNALTTL 252

Query: 430 EGLHRLLKLTVLDLSFNKISTTKALGQLVANYNSLQALNLLGNPI 473
            G+  L  L  LD+SFN IS    L + + + + L  L L GNPI
Sbjct: 253 RGIENLKSLEGLDVSFNLISDFSEL-EFLGSLSFLTDLWLEGNPI 295

BLAST of Carg25631 vs. TAIR 10
Match: AT2G34680.1 (Outer arm dynein light chain 1 protein )

HSP 1 Score: 58.5 bits (140), Expect = 2.0e-08
Identity = 40/124 (32.26%), Postives = 68/124 (54.84%), Query Frame = 0

Query: 350 INLSGNLIVHINSGS--LPKGLHTLNLSRNKISAIEGLKELTRLRILDLSYNRISRIG-H 409
           ++L G+ I  + SG   L   L  + L  N +S +EG++ L R+++LDLS+N     G  
Sbjct: 273 LDLRGHRIRSLTSGGLHLSPNLEFVYLRDNLLSTLEGIEILNRVKVLDLSFNDFKGPGFE 332

Query: 410 GLSNCAIIKELHLAGNKISDVEGLHRLLKLTVLDLSFNKISTTKALGQ-----LVANYNS 466
            L NC ++++L+LAGN+I+ +  L +L  L  L ++ NK+ +     Q     L A+ N 
Sbjct: 333 PLENCKMLQQLYLAGNQITSLASLPQLPNLEFLSVAQNKLKSLAMASQPRLQVLAASKNK 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037598.10.0e+00100.00Dynein assembly factor 1, axonemal, partial [Cucurbita argyrosperma subsp. argyr... [more]
KAG6608246.15.9e-30585.71Dynein assembly factor 1, axonemal, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_022941111.15.5e-30385.93uncharacterized protein LOC111446507 [Cucurbita moschata][more]
XP_022981325.14.4e-30085.22uncharacterized protein LOC111480488 [Cucurbita maxima][more]
XP_023524108.11.3e-29984.73uncharacterized protein LOC111788125 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q54Q391.0e-1231.89Protein phosphatase 1 regulatory subunit pprA OS=Dictyostelium discoideum OX=446... [more]
P360476.5e-1235.22Protein phosphatase 1 regulatory subunit SDS22 OS=Saccharomyces cerevisiae (stra... [more]
Q9D2H91.8e-0929.39Dynein axonemal assembly factor 1 OS=Mus musculus OX=10090 GN=Dnaaf1 PE=1 SV=1[more]
Q6AYH95.1e-0929.22Dynein axonemal assembly factor 1 OS=Rattus norvegicus OX=10116 GN=Dnaaf1 PE=1 S... [more]
B6D5P36.7e-0929.22Dynein axonemal assembly factor 1 OS=Peromyscus leucopus OX=10041 GN=Dnaaf1 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1FK882.7e-30385.93uncharacterized protein LOC111446507 OS=Cucurbita moschata OX=3662 GN=LOC1114465... [more]
A0A6J1IZ632.1e-30085.22uncharacterized protein LOC111480488 OS=Cucurbita maxima OX=3661 GN=LOC111480488... [more]
A0A1S3CQZ92.6e-24271.98uncharacterized protein LOC103503704 OS=Cucumis melo OX=3656 GN=LOC103503704 PE=... [more]
A0A0A0LDY61.0e-24171.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G889720 PE=4 SV=1[more]
A0A6J1BR661.4e-23871.86uncharacterized protein LOC111004961 isoform X1 OS=Momordica charantia OX=3673 G... [more]
Match NameE-valueIdentityDescription
AT1G78230.12.8e-11146.97Outer arm dynein light chain 1 protein [more]
AT4G03260.11.3e-6850.00Outer arm dynein light chain 1 protein [more]
AT4G03260.21.3e-6850.00Outer arm dynein light chain 1 protein [more]
AT3G17920.16.9e-0929.70Outer arm dynein light chain 1 protein [more]
AT2G34680.12.0e-0832.26Outer arm dynein light chain 1 protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003591Leucine-rich repeat, typical subtypeSMARTSM00369LRR_typ_2coord: 433..455
e-value: 73.0
score: 6.2
coord: 388..411
e-value: 0.0074
score: 25.5
coord: 344..367
e-value: 14.0
score: 12.2
NoneNo IPR availableSMARTSM00365LRR_sd22_2coord: 388..410
e-value: 2.8
score: 16.9
coord: 411..432
e-value: 430.0
score: 2.3
coord: 433..454
e-value: 49.0
score: 9.9
coord: 366..387
e-value: 38.0
score: 10.9
NoneNo IPR availablePFAMPF14580LRR_9coord: 348..452
e-value: 1.6E-8
score: 34.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 520..551
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 520..578
NoneNo IPR availablePANTHERPTHR15454:SF37OUTER ARM DYNEIN LIGHT CHAIN 1 PROTEINcoord: 3..253
NoneNo IPR availablePANTHERPTHR15454:SF37OUTER ARM DYNEIN LIGHT CHAIN 1 PROTEINcoord: 255..564
NoneNo IPR availablePANTHERPTHR15454NISCHARIN RELATEDcoord: 3..253
NoneNo IPR availablePANTHERPTHR15454NISCHARIN RELATEDcoord: 255..564
NoneNo IPR availableSUPERFAMILY52075Outer arm dynein light chain 1coord: 335..502
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 409..506
e-value: 2.3E-19
score: 71.2
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 293..408
e-value: 3.8E-17
score: 63.7
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 413..434
score: 7.519437
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 435..456
score: 7.65805
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 368..389
score: 8.951768
IPR001611Leucine-rich repeatPROSITEPS51450LRRcoord: 390..412
score: 7.827466

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25631-RACarg25631-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016020 membrane
molecular_function GO:0019901 protein kinase binding
molecular_function GO:0005515 protein binding