Carg25584 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25584
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiontranscription factor bHLH149-like
LocationCarg_Chr12: 9812667 .. 9813251 (-)
RNA-Seq ExpressionCarg25584
SyntenyCarg25584
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGACGCTCCGATTTCAGACGCCGCGGAATCGAAGAACTCGAGAAGGTTGTTGCAGCAGCCGAATAAGCGTCGGAAAATTGCTTCCGATGTTTGTGCGGAAGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGTGGAAGCGATCCGGCACGTAAGTCGGCGGCGGTCCTCGGCGCAAGCAGGATCTCCAATCAACGGACGATTAGTTCGCGAAACCGCGGATTGCGTTCTTGCCGCCGCAGCCAAGGGGAGGACTCGTTGGAGTCGCGCCATTCTAGCGAGCCGATTCCGCCAAAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGATCGGCCGTAAACCGTCGCGTATGGCGGAGACGAAGAGCGAGAAAAACAAGAAGTTGCCGGCAGTGCAGAGGAAGGTGAAAATTCTCGGCCGGTTAATTCCAGGTTGCCGTAAAGTCTCATTCCCGAACCTTCTGGAAGAAACGACCGATTACATCTCTGCTTTAGAGATGCAGGTAAGAGCCATGGCAGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAACTAA

mRNA sequence

ATGGACGACGCTCCGATTTCAGACGCCGCGGAATCGAAGAACTCGAGAAGGTTGTTGCAGCAGCCGAATAAGCGTCGGAAAATTGCTTCCGATGTTTGTGCGGAAGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGTGGAAGCGATCCGGCACGTAAGTCGGCGGCGGTCCTCGGCGCAAGCAGGATCTCCAATCAACGGACGATTAGTTCGCGAAACCGCGGATTGCGTTCTTGCCGCCGCAGCCAAGGGGAGGACTCGTTGGAGTCGCGCCATTCTAGCGAGCCGATTCCGCCAAAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGATCGGCCGTAAACCGTCGCGTATGGCGGAGACGAAGAGCGAGAAAAACAAGAAGTTGCCGGCAGTGCAGAGGAAGGTGAAAATTCTCGGCCGGTTAATTCCAGGTTGCCGTAAAGTCTCATTCCCGAACCTTCTGGAAGAAACGACCGATTACATCTCTGCTTTAGAGATGCAGGTAAGAGCCATGGCAGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAACTAA

Coding sequence (CDS)

ATGGACGACGCTCCGATTTCAGACGCCGCGGAATCGAAGAACTCGAGAAGGTTGTTGCAGCAGCCGAATAAGCGTCGGAAAATTGCTTCCGATGTTTGTGCGGAAGGCAGAGAACGGAAGTGGAGGACAGAGGTCGAGCAACAGATCTACTCTTCAAAGCTCGTGGAAGCGATCCGGCACGTAAGTCGGCGGCGGTCCTCGGCGCAAGCAGGATCTCCAATCAACGGACGATTAGTTCGCGAAACCGCGGATTGCGTTCTTGCCGCCGCAGCCAAGGGGAGGACTCGTTGGAGTCGCGCCATTCTAGCGAGCCGATTCCGCCAAAGGCTCGCGAGGCGTCGGAGGACGAAGAAATTGATCGGCCGTAAACCGTCGCGTATGGCGGAGACGAAGAGCGAGAAAAACAAGAAGTTGCCGGCAGTGCAGAGGAAGGTGAAAATTCTCGGCCGGTTAATTCCAGGTTGCCGTAAAGTCTCATTCCCGAACCTTCTGGAAGAAACGACCGATTACATCTCTGCTTTAGAGATGCAGGTAAGAGCCATGGCAGCGTTGGTCGAACTCCTCGCCGGAGCTCAAAGTAACTAA

Protein sequence

MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVELLAGAQSN
Homology
BLAST of Carg25584 vs. NCBI nr
Match: KAG7021037.1 (Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 357.5 bits (916), Expect = 7.5e-95
Identity = 194/194 (100.00%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH
Sbjct: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. NCBI nr
Match: XP_022937811.1 (transcription factor bHLH149-like [Cucurbita moschata])

HSP 1 Score: 357.1 bits (915), Expect = 9.8e-95
Identity = 193/194 (99.48%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH
Sbjct: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSRMAETKSEKNKKLPAVQRKVKILGRL+PGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. NCBI nr
Match: KAG6586210.1 (Transcription factor basic helix-loop-helix 149, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 355.9 bits (912), Expect = 2.2e-94
Identity = 192/194 (98.97%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH
Sbjct: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSRMAETKSEKNKKLP+VQRKVKILGRL+PGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRMAETKSEKNKKLPSVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. NCBI nr
Match: XP_023537600.1 (transcription factor bHLH149-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 349.4 bits (895), Expect = 2.0e-92
Identity = 189/194 (97.42%), Postives = 191/194 (98.45%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDA ESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIR 
Sbjct: 1   MDDAPISDAEESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRQ 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAA AKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAVAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSRMAETKSEKNKKLPAVQRKVKILGRL+PGCRK+SFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLVPGCRKLSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. NCBI nr
Match: XP_022965958.1 (transcription factor bHLH149-like [Cucurbita maxima])

HSP 1 Score: 340.5 bits (872), Expect = 9.5e-90
Identity = 186/194 (95.88%), Postives = 190/194 (97.94%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDA ESKNSRRLLQ+PNKRRKIA DV A+GRERKWRTEVEQQIYSSKLVEAIR 
Sbjct: 1   MDDAPISDAEESKNSRRLLQKPNKRRKIAFDVGAKGRERKWRTEVEQQIYSSKLVEAIRQ 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSR+AETKSEKNKKLPAVQRKVKILGRL+PGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRIAETKSEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. ExPASy Swiss-Prot
Match: O80482 (Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.1e-35
Identity = 97/192 (50.52%), Postives = 126/192 (65.62%), Query Frame = 0

Query: 6   ISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRR 65
           I +  ES   ++         +I +    E   ++W+T   QQIY+ KLVEA+R V R+R
Sbjct: 9   IENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACKLVEALRRV-RQR 68

Query: 66  SSAQAGSPIN------GRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL 125
           SS  + +  +       R +R+TAD VLAA+A+G TRWSRAILASR R +L + R+ KK 
Sbjct: 69  SSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRVRAKLKKHRKAKKS 128

Query: 126 IGRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVR 185
            G   SR   T++ +  KLPAV+RK+KILGRL+PGCRKVS PNLL+E TDYI+ALEMQVR
Sbjct: 129 TGNCKSRKGLTETNR-IKLPAVERKLKILGRLVPGCRKVSVPNLLDEATDYIAALEMQVR 188

Query: 186 AMAALVELLAGA 192
           AM AL ELL  A
Sbjct: 189 AMEALAELLTAA 198

BLAST of Carg25584 vs. ExPASy Swiss-Prot
Match: Q9LSN7 (Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.8e-28
Identity = 86/200 (43.00%), Postives = 119/200 (59.50%), Query Frame = 0

Query: 5   PISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIR--HVS 64
           P + ++ S  SRR  ++ +    +           KWR+E +QQIYS+KLV A+R   +S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 65  RRRSSAQAGS-PINGRLVRETADCVLAAAAKGRTRWSRAILASRF--------RQRLARR 124
           ++ SS+ + S P  GR VRE AD  LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 125 RRTKKLIG--RKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYI 184
             T    G  R   + A     K K LPAVQRKVK+L RL+PGCRK S P +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 185 SALEMQVRAMAALVELLAGA 192
           +A+EMQ+R M A++  ++ +
Sbjct: 194 AAMEMQIRTMTAILSAVSSS 213

BLAST of Carg25584 vs. ExPASy Swiss-Prot
Match: Q9C8Z9 (Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 3.1e-27
Identity = 80/180 (44.44%), Postives = 108/180 (60.00%), Query Frame = 0

Query: 26  RKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSSAQAGSPI---NGRLVRET 85
           R  AS V  E   R WR+E +Q+IYS+KL +A++ V    S++ + SP     G+ VRE 
Sbjct: 34  RSSASSVSGEIHAR-WRSEKQQRIYSAKLFQALQQVRLNSSASTSSSPTAQKRGKAVREA 93

Query: 86  ADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI-------------GRKPSRMAE 145
           AD  LA +A+GRT WSRAILA+R + +  ++RR +  +              R   R   
Sbjct: 94  ADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAMTTVVSSSSNRSRKRRVS 153

Query: 146 TKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVELLA 190
                 K +P V RKV++LGRL+PGC K S P +LEE TDYI ALEMQVRAM +LV+LL+
Sbjct: 154 VLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQALEMQVRAMNSLVQLLS 212

BLAST of Carg25584 vs. ExPASy Swiss-Prot
Match: Q9M9L6 (Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 76/176 (43.18%), Postives = 104/176 (59.09%), Query Frame = 0

Query: 17  RLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSSAQAGSPING 76
           +L++   + R  + +       R+WR    Q++YS KL +A++   R R SA        
Sbjct: 39  KLMEALRRSRVSSEEAPVRHLSRRWRATTAQKVYSLKLYDALQ---RSRRSA-------- 98

Query: 77  RLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGRKPSRMAETKSEKNK 136
             VR+TAD VLA  A+G TRWSRAIL SRF   L RRR TK       +      S + +
Sbjct: 99  -TVRDTADKVLATTARGATRWSRAILVSRFGTSLRRRRNTKPASALAAAIRGSGGSGRRR 158

Query: 137 KLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVELLAGAQ 193
           KL AV  +V++LG L+PGCR+ + P LL+ET DYI+ALEMQVRAM AL ++L+  Q
Sbjct: 159 KLSAVGNRVRVLGGLVPGCRRTALPELLDETADYIAALEMQVRAMTALSKILSELQ 202

BLAST of Carg25584 vs. ExPASy TrEMBL
Match: A0A6J1FBF1 (transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111444091 PE=4 SV=1)

HSP 1 Score: 357.1 bits (915), Expect = 4.8e-95
Identity = 193/194 (99.48%), Postives = 194/194 (100.00%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH
Sbjct: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSRMAETKSEKNKKLPAVQRKVKILGRL+PGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. ExPASy TrEMBL
Match: A0A6J1HQH2 (transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111465681 PE=4 SV=1)

HSP 1 Score: 340.5 bits (872), Expect = 4.6e-90
Identity = 186/194 (95.88%), Postives = 190/194 (97.94%), Query Frame = 0

Query: 1   MDDAPISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRH 60
           MDDAPISDA ESKNSRRLLQ+PNKRRKIA DV A+GRERKWRTEVEQQIYSSKLVEAIR 
Sbjct: 1   MDDAPISDAEESKNSRRLLQKPNKRRKIAFDVGAKGRERKWRTEVEQQIYSSKLVEAIRQ 60

Query: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120
           VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI
Sbjct: 61  VSRRRSSAQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI 120

Query: 121 GRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRA 180
           GRKPSR+AETKSEKNKKLPAVQRKVKILGRL+PGCRKVSFPNLLEETTDYISALEMQVRA
Sbjct: 121 GRKPSRIAETKSEKNKKLPAVQRKVKILGRLVPGCRKVSFPNLLEETTDYISALEMQVRA 180

Query: 181 MAALVELLAGAQSN 195
           MAALVELLAGAQSN
Sbjct: 181 MAALVELLAGAQSN 194

BLAST of Carg25584 vs. ExPASy TrEMBL
Match: A0A6J1KCR3 (transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111493732 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 7.4e-72
Identity = 154/187 (82.35%), Postives = 169/187 (90.37%), Query Frame = 0

Query: 8   DAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSS 67
           DA ESKNSRR LQ+PNKR +IAS+V AEGRER+WRTEVEQQIYSS+L EA++ VSR+RSS
Sbjct: 2   DAEESKNSRRSLQKPNKRLQIASNVSAEGRERRWRTEVEQQIYSSRLAEALQRVSRQRSS 61

Query: 68  AQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGRKPSRM 127
             A S INGRLVRETADCVLAAAAKGRTRWSRAILA+RFRQRLARRRRTKK IGRKPSR+
Sbjct: 62  PHARSTINGRLVRETADCVLAAAAKGRTRWSRAILANRFRQRLARRRRTKKSIGRKPSRV 121

Query: 128 AETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVEL 187
            E    KN+KLPAVQRKVKILGRL+PGCRK+SFPNLLEE TDYISALEMQV+AMAAL E+
Sbjct: 122 EEV---KNRKLPAVQRKVKILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMAALAEI 181

Query: 188 LAGAQSN 195
           LAGAQSN
Sbjct: 182 LAGAQSN 185

BLAST of Carg25584 vs. ExPASy TrEMBL
Match: A0A6J1G430 (transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111450552 PE=4 SV=1)

HSP 1 Score: 276.9 bits (707), Expect = 6.2e-71
Identity = 152/187 (81.28%), Postives = 167/187 (89.30%), Query Frame = 0

Query: 8   DAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSS 67
           DA ESKNS+R LQ+PNKRR+IASDV AEGRER+WRTEVEQQIYSS+L EA++ V R  SS
Sbjct: 2   DAEESKNSKRSLQKPNKRRQIASDVSAEGRERRWRTEVEQQIYSSRLAEALQRVGRLPSS 61

Query: 68  AQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLIGRKPSRM 127
             A S INGRLVRETADCVLAAAAKGRTRWSRAILA+RFRQRLARRRRTKK IGRKPSR+
Sbjct: 62  PHARSTINGRLVRETADCVLAAAAKGRTRWSRAILANRFRQRLARRRRTKKSIGRKPSRV 121

Query: 128 AETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVEL 187
           AE    KN+KLPAVQRKV+ILGRL+PGCRK+SFPNLLEE TDYISALEMQV+AMAAL E+
Sbjct: 122 AEV---KNRKLPAVQRKVRILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMAALAEI 181

Query: 188 LAGAQSN 195
           LAGAQ N
Sbjct: 182 LAGAQRN 185

BLAST of Carg25584 vs. ExPASy TrEMBL
Match: A0A0A0LJ14 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034620 PE=4 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 1.2e-50
Identity = 124/189 (65.61%), Postives = 145/189 (76.72%), Query Frame = 0

Query: 8   DAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSS 67
           DA +S NS+ LLQ  NK   +   +  E  + +W+TE++QQIYSS+L +A+R V+  RSS
Sbjct: 2   DADDSNNSKTLLQHSNK--CLPKILTPEPTQHRWKTEIQQQIYSSRLTQALRRVTHPRSS 61

Query: 68  AQAGSPINGRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRR--TKKLIGRKPS 127
               SP+NG LVR TAD VLAA AKGRTRWSRAILA+RFRQ LARRRR   KKL+ RKP 
Sbjct: 62  ----SPLNGNLVRRTADSVLAATAKGRTRWSRAILATRFRQSLARRRRRTKKKLLARKP- 121

Query: 128 RMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALV 187
              E K+EK +KLPAVQRKVKILGRL+PGCRK+SFPNLLEE TDYISALEMQV+AM AL 
Sbjct: 122 ---ELKTEKVRKLPAVQRKVKILGRLVPGCRKLSFPNLLEEATDYISALEMQVKAMTALA 180

Query: 188 ELLAGAQSN 195
           ELLAG Q N
Sbjct: 182 ELLAGNQRN 180

BLAST of Carg25584 vs. TAIR 10
Match: AT1G09250.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 149.4 bits (376), Expect = 2.9e-36
Identity = 97/192 (50.52%), Postives = 126/192 (65.62%), Query Frame = 0

Query: 6   ISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRR 65
           I +  ES   ++         +I +    E   ++W+T   QQIY+ KLVEA+R V R+R
Sbjct: 9   IENTGESSRRKKPRISETAEAEIEARRVNEESLKRWKTNRVQQIYACKLVEALRRV-RQR 68

Query: 66  SSAQAGSPIN------GRLVRETADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKL 125
           SS  + +  +       R +R+TAD VLAA+A+G TRWSRAILASR R +L + R+ KK 
Sbjct: 69  SSTTSNNETDKLVSGAAREIRDTADRVLAASARGTTRWSRAILASRVRAKLKKHRKAKKS 128

Query: 126 IGRKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVR 185
            G   SR   T++ +  KLPAV+RK+KILGRL+PGCRKVS PNLL+E TDYI+ALEMQVR
Sbjct: 129 TGNCKSRKGLTETNR-IKLPAVERKLKILGRLVPGCRKVSVPNLLDEATDYIAALEMQVR 188

Query: 186 AMAALVELLAGA 192
           AM AL ELL  A
Sbjct: 189 AMEALAELLTAA 198

BLAST of Carg25584 vs. TAIR 10
Match: AT3G17100.1 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-29
Identity = 86/200 (43.00%), Postives = 119/200 (59.50%), Query Frame = 0

Query: 5   PISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIR--HVS 64
           P + ++ S  SRR  ++ +    +           KWR+E +QQIYS+KLV A+R   +S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 65  RRRSSAQAGS-PINGRLVRETADCVLAAAAKGRTRWSRAILASRF--------RQRLARR 124
           ++ SS+ + S P  GR VRE AD  LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 125 RRTKKLIG--RKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYI 184
             T    G  R   + A     K K LPAVQRKVK+L RL+PGCRK S P +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 185 SALEMQVRAMAALVELLAGA 192
           +A+EMQ+R M A++  ++ +
Sbjct: 194 AAMEMQIRTMTAILSAVSSS 213

BLAST of Carg25584 vs. TAIR 10
Match: AT3G17100.2 (sequence-specific DNA binding transcription factors )

HSP 1 Score: 126.7 bits (317), Expect = 2.0e-29
Identity = 86/200 (43.00%), Postives = 119/200 (59.50%), Query Frame = 0

Query: 5   PISDAAESKNSRRLLQQPNKRRKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIR--HVS 64
           P + ++ S  SRR  ++ +    +           KWR+E +QQIYS+KLV A+R   +S
Sbjct: 14  PTTTSSNSDRSRRKRKKKSSPSSVEKSPSPSISLEKWRSEKQQQIYSTKLVHALRELRIS 73

Query: 65  RRRSSAQAGS-PINGRLVRETADCVLAAAAKGRTRWSRAILASRF--------RQRLARR 124
           ++ SS+ + S P  GR VRE AD  LA AA+G+T WSRAIL+           RQR++  
Sbjct: 74  QQPSSSSSSSIPRGGRAVREVADRALAVAARGKTLWSRAILSKAVKLKFRKHKRQRISNP 133

Query: 125 RRTKKLIG--RKPSRMAETKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYI 184
             T    G  R   + A     K K LPAVQRKVK+L RL+PGCRK S P +LEETTDYI
Sbjct: 134 TTTTLTTGSIRSKKQRATVLRLKAKGLPAVQRKVKVLSRLVPGCRKQSLPVVLEETTDYI 193

Query: 185 SALEMQVRAMAALVELLAGA 192
           +A+EMQ+R M A++  ++ +
Sbjct: 194 AAMEMQIRTMTAILSAVSSS 213

BLAST of Carg25584 vs. TAIR 10
Match: AT3G06590.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-28
Identity = 80/180 (44.44%), Postives = 108/180 (60.00%), Query Frame = 0

Query: 26  RKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSSAQAGSPI---NGRLVRET 85
           R  AS V  E   R WR+E +Q+IYS+KL +A++ V    S++ + SP     G+ VRE 
Sbjct: 34  RSSASSVSGEIHAR-WRSEKQQRIYSAKLFQALQQVRLNSSASTSSSPTAQKRGKAVREA 93

Query: 86  ADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI-------------GRKPSRMAE 145
           AD  LA +A+GRT WSRAILA+R + +  ++RR +  +              R   R   
Sbjct: 94  ADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAMTTVVSSSSNRSRKRRVS 153

Query: 146 TKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVELLA 190
                 K +P V RKV++LGRL+PGC K S P +LEE TDYI ALEMQVRAM +LV+LL+
Sbjct: 154 VLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQALEMQVRAMNSLVQLLS 212

BLAST of Carg25584 vs. TAIR 10
Match: AT3G06590.2 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 123.2 bits (308), Expect = 2.2e-28
Identity = 80/180 (44.44%), Postives = 108/180 (60.00%), Query Frame = 0

Query: 26  RKIASDVCAEGRERKWRTEVEQQIYSSKLVEAIRHVSRRRSSAQAGSPI---NGRLVRET 85
           R  AS V  E   R WR+E +Q+IYS+KL +A++ V    S++ + SP     G+ VRE 
Sbjct: 34  RSSASSVSGEIHAR-WRSEKQQRIYSAKLFQALQQVRLNSSASTSSSPTAQKRGKAVREA 93

Query: 86  ADCVLAAAAKGRTRWSRAILASRFRQRLARRRRTKKLI-------------GRKPSRMAE 145
           AD  LA +A+GRT WSRAILA+R + +  ++RR +  +              R   R   
Sbjct: 94  ADRALAVSARGRTLWSRAILANRIKLKFRKQRRPRATMAIPAMTTVVSSSSNRSRKRRVS 153

Query: 146 TKSEKNKKLPAVQRKVKILGRLIPGCRKVSFPNLLEETTDYISALEMQVRAMAALVELLA 190
                 K +P V RKV++LGRL+PGC K S P +LEE TDYI ALEMQVRAM +LV+LL+
Sbjct: 154 VLRLNKKSIPDVNRKVRVLGRLVPGCGKQSVPVILEEATDYIQALEMQVRAMNSLVQLLS 212

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7021037.17.5e-95100.00Transcription factor bHLH, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022937811.19.8e-9599.48transcription factor bHLH149-like [Cucurbita moschata][more]
KAG6586210.12.2e-9498.97Transcription factor basic helix-loop-helix 149, partial [Cucurbita argyrosperma... [more]
XP_023537600.12.0e-9297.42transcription factor bHLH149-like [Cucurbita pepo subsp. pepo][more]
XP_022965958.19.5e-9095.88transcription factor bHLH149-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O804824.1e-3550.52Transcription factor bHLH149 OS=Arabidopsis thaliana OX=3702 GN=BHLH149 PE=1 SV=... [more]
Q9LSN72.8e-2843.00Transcription factor bHLH147 OS=Arabidopsis thaliana OX=3702 GN=BHLH147 PE=1 SV=... [more]
Q9C8Z93.1e-2744.44Transcription factor bHLH148 OS=Arabidopsis thaliana OX=3702 GN=BHLH148 PE=1 SV=... [more]
Q9M9L61.7e-2543.18Transcription factor bHLH150 OS=Arabidopsis thaliana OX=3702 GN=BHLH150 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
A0A6J1FBF14.8e-9599.48transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111444091 ... [more]
A0A6J1HQH24.6e-9095.88transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111465681 PE... [more]
A0A6J1KCR37.4e-7282.35transcription factor bHLH149-like OS=Cucurbita maxima OX=3661 GN=LOC111493732 PE... [more]
A0A6J1G4306.2e-7181.28transcription factor bHLH149-like OS=Cucurbita moschata OX=3662 GN=LOC111450552 ... [more]
A0A0A0LJ141.2e-5065.61Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034620 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09250.12.9e-3650.52basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G17100.12.0e-2943.00sequence-specific DNA binding transcription factors [more]
AT3G17100.22.0e-2943.00sequence-specific DNA binding transcription factors [more]
AT3G06590.12.2e-2844.44basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
AT3G06590.22.2e-2844.44basic helix-loop-helix (bHLH) DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..31
NoneNo IPR availablePANTHERPTHR33124:SF69TRANSCRIPTION FACTOR BHLH149coord: 16..191
IPR044660Transcription factor IBH1-likePANTHERPTHR33124TRANSCRIPTION FACTOR IBH1-LIKE 1coord: 16..191
IPR044549Transcription factor IBH1-like, bHLH domainCDDcd11444bHLH_AtIBH1_likecoord: 131..185
e-value: 2.10724E-16
score: 68.0328
IPR036638Helix-loop-helix DNA-binding domain superfamilySUPERFAMILY47459HLH, helix-loop-helix DNA-binding domaincoord: 139..189

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25584-RACarg25584-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0046983 protein dimerization activity
molecular_function GO:0000976 transcription cis-regulatory region binding