Carg25502 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25502
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome P450 81D1-like
Location	Carg_Chr20: 5736900 .. 5739109 (-)
RNA-Seq Expression	Carg25502
Synteny	Carg25502

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCAGATAAGCTCCCTTGAGATTTTCTCAACAAATCACCTCAATTTGTTCTTGGGAATCTGGGAAGAGGAAATCAAATATTTGCTATGTAAATTATTTGGGAATTCATTGGAAGATGAGTTCAAGGTCGTGGAATTGGAGCTCATGTTGCTTGATGGTGTTGTAAATTGTCATAAGTTTTTTATTTAATTTAGTTATTAACTCCCGTTAATTTATAAGTGTGATTAAATAAGAATATTATTAATTTAGTAGAGTCCTACGAGTTTTTAGTAACCACTCTACTAAATGGAGCCTATGATATTGATATTGATTTTGAAGATGTTTCAAATGTGTTGATGATTGATGAGGTGCGAAAGAAGGACAAGGAGACATATCAAGACTCTAGCACAAATGTTCTCACTGCTTTGTAAGGACTTATACTTGGAAAAAGGGCGAACGTGAAAAGTCTGGATAAAAGTCGAAAGGTAGATTTGATAATTGGAGAAAGCTTGATAAAAATGAGTGTGCATATTGTCGACAGAAAGGCCATTAGAAGAAATATTGCCCGACCTTAAAAGAGAAAGGGTCGAAGTCCAATGTGGTTAGAGATGATGACAATGAAGCAGATAATGTCTCGAGGATTTCCTTATCCGTCAACCAAATTAGTGAATGGATTCTTGATTCTGGGTGATAGGTGTGTCCCAATAGAGAGATGTTTTTGGACTTCAAACAGTTCAATGGTAGAGTTGTCTATATAGGCAATGATAGTACTTGCAAGATGATGAGAATTGGCTCAGTACAAATCAAGATGTTTGATGGGTTGTACAAAAACTCAATGATGTGAGGTATGTCTCTGACTTGAAGAAAAATTTGATTTCCCTTGGCGTTGTGGATGCGAATGGTTATCATATCATTTTGGAATGAGGGAATTTGAAAGTAGCTCGTAGAGCTTTGGTGGCATTTAAAGGGACTAGAAGAGGTAGCATATACTACCTTAACGGAACCACGATCATTGGGCATGCTGCTGTGGCAAGTTCGAAAGAACAAGACATGTATATCAAAGTTATGGCACATGCGACTCGGGCATGCTGGTGAAAAGGCGCTCCAGACATTGGTGAATCAAGGAGTCCTTAAGGGTGCCACAACTGGTAAAATTGACTTTGGTGAACAAAGCAAAAAAAAAATGTGCAGTTTGGTATTGCTATACATCAAATAGAGAGCATCTTGGCATCTTGGATTATGTTCACACTGATGCATGGGGTACCACAAGAATATCTCATTGGGAGGAAAAAGGTGGTTTGTCACCTTTATTGATGATTACTCGATGAGAGTTTGGATGTATCCCATGGGCATAAAAAACGAGATTTTCCAAACTTCCTGGAGTGGAAGAAAATGGTTGAGAATAAAACAGAGATTAGACAACAGTGGAGAATATACTTATGATTCATTCCTTAAAATATGCTGAGATGAAGGGATCATTCGACACTTCACTATTCCTGGTAATCCATAACAAAATGGAGTTGTTGAGAAAATGAACCAGACATTGATAGAGAAGGTTCGATGCATATTGTCTCAAGCAGGATTGAGGAAGGAATTTTGGGTTGAGGCTCTCAATTACGCAGTTCACTTGATGAATCATTTACCTATTTCTGGAAATGGTGGAAAAAATTCGCTCGAGGTATGGTCAAGTACTCTTGTCATACATTATGATAATTTACATGTGTTTGGTTATCCTGCTTAGTATCATGTGACAAACTCAAAATTAGATCCTAGAGCAAAAAAAAAAAAAAAAAAAAAAGGTCAAGTTTATGGGCTTTAGCGAAGGCATTAAGGGCTATAGGTTATGGTATCCAAAAGCAAGTAAAATTGTCAATAGTCGGGATGTGACATTCGATAAGTCTGGAATGTCTTTGCAGAAAATTGAAAATAATGACGAGGCATCGACGCAAGTGGAGAAGGTGGTATCCAAAAGGGCTATGGAAAATAATGACGACGCAAGTGGAGAAGGCATTAAGGGCTATAGGTTATGGTATCCAAAAGCAAGTAAAATTGTCAATAGTCGGGAGAGACCACGAAGGGAATTTTGAAAACCTGCAAGGTTTGATGATATGATAGCATATGTTTTCCCTATGATTGATGGAGTTTCCGACTGTTACAAAGATGTTATTGGGCTGGCAAGAGGCTATGAATGA

mRNA sequence

ATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCAGATAAGCTCCCTTGAGATTTTCTCAACAAATCACCTCAATTTGTTCTTGGGAATCTGGGAAGAGGAAATCAAATATTTGCTATGTAAATTATTTGGGAATTCATTGGAAGATGAGTTCAAGGTCGTGGAATTGGAGCTCATGTTGCTTGATGGTGTTGGACTAGAAGAGGTAGCATATACTACCTTAACGGAACCACGATCATTGGGCATGCTGCTGTGGCAAGTTCGAAAGAACAAGACATGTATATCAAAGTTATGGCACATGCGACTCGGGCATGCTGGTGAAAAGGCGCTCCAGACATTGGTGAATCAAGGAGTCCTTAAGGGTGCCACAACTGGATTGAGGAAGGAATTTTGGGTTGAGGCTCTCAATTACGCAGTTCACTTGATGAATCATTTACCTATTTCTGGAAATGGTGGAAAAAATTCGCTCGAGAAAATTGAAAATAATGACGAGGCATCGACGCAAGTGGAGAAGGTGGTATCCAAAAGGGCTATGGAAAATAATGACGACGCAAGTGGAGAAGGCATTAAGGGCTATAGGTTTGATGATATGATAGCATATGTTTTCCCTATGATTGATGGAGTTTCCGACTGTTACAAAGATGTTATTGGGCTGGCAAGAGGCTATGAATGA

Coding sequence (CDS)

ATGTCGGTCTCCCCTTACGGCGAACATTGGAGAAACCTCCGCCAGATAAGCTCCCTTGAGATTTTCTCAACAAATCACCTCAATTTGTTCTTGGGAATCTGGGAAGAGGAAATCAAATATTTGCTATGTAAATTATTTGGGAATTCATTGGAAGATGAGTTCAAGGTCGTGGAATTGGAGCTCATGTTGCTTGATGGTGTTGGACTAGAAGAGGTAGCATATACTACCTTAACGGAACCACGATCATTGGGCATGCTGCTGTGGCAAGTTCGAAAGAACAAGACATGTATATCAAAGTTATGGCACATGCGACTCGGGCATGCTGGTGAAAAGGCGCTCCAGACATTGGTGAATCAAGGAGTCCTTAAGGGTGCCACAACTGGATTGAGGAAGGAATTTTGGGTTGAGGCTCTCAATTACGCAGTTCACTTGATGAATCATTTACCTATTTCTGGAAATGGTGGAAAAAATTCGCTCGAGAAAATTGAAAATAATGACGAGGCATCGACGCAAGTGGAGAAGGTGGTATCCAAAAGGGCTATGGAAAATAATGACGACGCAAGTGGAGAAGGCATTAAGGGCTATAGGTTTGATGATATGATAGCATATGTTTTCCCTATGATTGATGGAGTTTCCGACTGTTACAAAGATGTTATTGGGCTGGCAAGAGGCTATGAATGA

Protein sequence

MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELELMLLDGVGLEEVAYTTLTEPRSLGMLLWQVRKNKTCISKLWHMRLGHAGEKALQTLVNQGVLKGATTGLRKEFWVEALNYAVHLMNHLPISGNGGKNSLEKIENNDEASTQVEKVVSKRAMENNDDASGEGIKGYRFDDMIAYVFPMIDGVSDCYKDVIGLARGYE

Homology

[Close]

BLAST of Carg25502 vs. NCBI nr
Match: KAG7011038.1 (Cytochrome P450 81F2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 459.5 bits (1181), Expect = 1.6e-125
Identity = 226/226 (100.00%), Postives = 226/226 (100.00%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE
Sbjct: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60

Query: 61  LMLLDGVGLEEVAYTTLTEPRSLGMLLWQVRKNKTCISKLWHMRLGHAGEKALQTLVNQG 120
           LMLLDGVGLEEVAYTTLTEPRSLGMLLWQVRKNKTCISKLWHMRLGHAGEKALQTLVNQG
Sbjct: 61  LMLLDGVGLEEVAYTTLTEPRSLGMLLWQVRKNKTCISKLWHMRLGHAGEKALQTLVNQG 120

Query: 121 VLKGATTGLRKEFWVEALNYAVHLMNHLPISGNGGKNSLEKIENNDEASTQVEKVVSKRA 180
           VLKGATTGLRKEFWVEALNYAVHLMNHLPISGNGGKNSLEKIENNDEASTQVEKVVSKRA
Sbjct: 121 VLKGATTGLRKEFWVEALNYAVHLMNHLPISGNGGKNSLEKIENNDEASTQVEKVVSKRA 180

Query: 181 MENNDDASGEGIKGYRFDDMIAYVFPMIDGVSDCYKDVIGLARGYE 227
           MENNDDASGEGIKGYRFDDMIAYVFPMIDGVSDCYKDVIGLARGYE
Sbjct: 181 MENNDDASGEGIKGYRFDDMIAYVFPMIDGVSDCYKDVIGLARGYE 226

[Close]

BLAST of Carg25502 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-20
Identity = 56/65 (86.15%), Postives = 59/65 (90.77%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+ISSLEIFSTN LNLFLGI EEEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. NCBI nr
Match: KAG7011035.1 (Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-20
Identity = 56/65 (86.15%), Postives = 59/65 (90.77%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+ISSLEIFSTN LNLFLGI EEEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 110.9 bits (276), Expect = 1.4e-20
Identity = 56/65 (86.15%), Postives = 59/65 (90.77%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+ISSLEIFSTN LNLFLGI EEEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-19
Identity = 53/65 (81.54%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+I SLEIFSTN LNLFLG+ +EEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.6e-09
Identity = 30/53 (56.60%), Postives = 40/53 (75.47%), Query Frame = 0


Query: 4   SPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKV 57
           SPYGEHWRNLR+++++EIFS   LN FL I E+E+K LL  L+ +S +D  KV
Sbjct: 119 SPYGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKV 171

[Close]

BLAST of Carg25502 vs. ExPASy Swiss-Prot
Match: Q9LVD6 (Cytochrome P450 81F2 OS=Arabidopsis thaliana OX=3702 GN=CYP81F2 PE=2 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 3.8e-08
Identity = 33/65 (50.77%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           +  + YG+HWRNLR+I SLEI S+N L  FL + ++EI+ LL KL   S E + +VVELE
Sbjct: 114 IGTAAYGDHWRNLRRICSLEILSSNRLTGFLSVRKDEIRRLLTKL---SREYDGRVVELE 173

Query: 61  LMLLD 66
            +L D
Sbjct: 174 PLLAD 175

[Close]

BLAST of Carg25502 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 8.4e-08
Identity = 29/65 (44.62%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           M  +PYG+HWRNLR++ ++EIFST+ LN FL +  +E++ L+ +L       +  VVEL+
Sbjct: 123 MIAAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELK 182

Query: 61  LMLLD 66
            ML+D
Sbjct: 183 PMLMD 186

[Close]

BLAST of Carg25502 vs. ExPASy Swiss-Prot
Match: Q0WTF4 (Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 7.1e-07
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           +  +PYG+HWRNLR+I SLEI S+N L  FL I ++EI  +L +L      D  K +ELE
Sbjct: 115 VGTAPYGDHWRNLRRICSLEILSSNRLTNFLHIRKDEIHRMLTRL----SRDVNKEIELE 174

Query: 61  LMLLD 66
            +L D
Sbjct: 175 PLLSD 175

[Close]

BLAST of Carg25502 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 2.1e-06
Identity = 31/59 (52.54%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 4   SPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELELM 63
           SPYG+HWRNLR+I S+EI S++ LN FL I  +EI  L+ KL   S  + F  VEL  M
Sbjct: 118 SPYGDHWRNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSY-NGFTEVELRPM 175

[Close]

BLAST of Carg25502 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 110.9 bits (276), Expect = 6.9e-21
Identity = 56/65 (86.15%), Postives = 59/65 (90.77%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+ISSLEIFSTN LNLFLGI EEEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 107.5 bits (267), Expect = 7.6e-20
Identity = 53/65 (81.54%), Postives = 58/65 (89.23%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+I SLEIFSTN LNLFLG+ +EEIK LLCKL G+SLED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 1.1e-18
Identity = 52/65 (80.00%), Postives = 55/65 (84.62%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           MSVSPYGEHWRNLR+I SLEIFSTN LN FLG+  EEIK LLCKL GN LED F+VVELE
Sbjct: 114 MSVSPYGEHWRNLRRIVSLEIFSTNRLNFFLGVRGEEIKCLLCKLCGNLLEDRFRVVELE 173

Query: 61  LMLLD 66
            MLLD
Sbjct: 174 PMLLD 178

[Close]

BLAST of Carg25502 vs. ExPASy TrEMBL
Match: A0A0A0LQI5 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.9e-15
Identity = 48/66 (72.73%), Postives = 53/66 (80.30%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGN-SLEDEFKVVEL 60
           M+VSPYGEHWRNLR+I +LEIFST  LN F  I EEE+K LLCKL GN   EDEF+VVEL
Sbjct: 115 MAVSPYGEHWRNLRRICTLEIFSTTRLNSFSRIREEEVKRLLCKLCGNYKFEDEFRVVEL 174

Query: 61  ELMLLD 66
           E MLLD
Sbjct: 175 ESMLLD 180

[Close]

BLAST of Carg25502 vs. ExPASy TrEMBL
Match: A0A6J1D5S6 (isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 9.7e-15
Identity = 46/65 (70.77%), Postives = 57/65 (87.69%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           ++VSPYGEHWRNLR+IS+LEIFST+ LNLFLGI E+E+K LL KL G+SL+ EF+ VEL+
Sbjct: 194 LAVSPYGEHWRNLRRISTLEIFSTSRLNLFLGIREDEVKNLLRKLRGDSLQ-EFRAVELD 253

Query: 61  LMLLD 66
            MLLD
Sbjct: 254 SMLLD 257

[Close]

BLAST of Carg25502 vs. TAIR 10
Match: AT2G23190.1 (cytochrome P450, family 81, subfamily D, polypeptide 7 )

HSP 1 Score: 63.5 bits (153), Expect = 2.4e-10
Identity = 31/65 (47.69%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           M+ +PYG+HWRNLR+I +LEIFS++ LN FL + ++EI++LL +L  NS +  F  VE+ 
Sbjct: 161 MTSAPYGDHWRNLRRIGTLEIFSSHKLNGFLSVRKDEIRHLLLRLSKNS-QHGFAKVEMR 220

Query: 61  LMLLD 66
            +  D
Sbjct: 221 QLFYD 224

[Close]

BLAST of Carg25502 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 61.6 bits (148), Expect = 9.3e-10
Identity = 36/72 (50.00%), Postives = 47/72 (65.28%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           M  +PYG+HWRNLR+I++LE+FSTN LN    I  +E+K LL KL   S+E   K VEL 
Sbjct: 116 MVTAPYGDHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVERPAK-VELR 175

Query: 61  LMLLDGVGLEEV 73
             LL G+ L  +
Sbjct: 176 -QLLTGLTLNVI 185

[Close]

BLAST of Carg25502 vs. TAIR 10
Match: AT2G23220.1 (cytochrome P450, family 81, subfamily D, polypeptide 6 )

HSP 1 Score: 60.8 bits (146), Expect = 1.6e-09
Identity = 30/65 (46.15%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           M+ +PYG+HWRNLR+I++LEIF+++ LN FL + ++EI+ LL +L  NS    F  VE+ 
Sbjct: 132 MTSAPYGDHWRNLRRIATLEIFASHKLNGFLSVRKDEIRQLLLRLSKNS-RHGFAKVEMR 191

Query: 61  LMLLD 66
            +L +
Sbjct: 192 HLLFE 195

[Close]

BLAST of Carg25502 vs. TAIR 10
Match: AT5G57220.1 (cytochrome P450, family 81, subfamily F, polypeptide 2 )

HSP 1 Score: 60.1 bits (144), Expect = 2.7e-09
Identity = 33/65 (50.77%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           +  + YG+HWRNLR+I SLEI S+N L  FL + ++EI+ LL KL   S E + +VVELE
Sbjct: 114 IGTAAYGDHWRNLRRICSLEILSSNRLTGFLSVRKDEIRRLLTKL---SREYDGRVVELE 173

Query: 61  LMLLD 66
            +L D
Sbjct: 174 PLLAD 175

[Close]

BLAST of Carg25502 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 59.3 bits (142), Expect = 4.6e-09
Identity = 31/65 (47.69%), Postives = 44/65 (67.69%), Query Frame = 0


Query: 1   MSVSPYGEHWRNLRQISSLEIFSTNHLNLFLGIWEEEIKYLLCKLFGNSLEDEFKVVELE 60
           M  + YG+HWRNLR+I S+EIFS + LN FL I ++EI+ L+ +L  N    EF  V+++
Sbjct: 116 MIAASYGDHWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRN-FSQEFVKVDMK 175

Query: 61  LMLLD 66
            ML D
Sbjct: 176 SMLSD 179

The following BLAST results are available for this feature:

BLAST of Carg25502 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011038.1	1.6e-125	100.00	Cytochrome P450 81F2, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022943757.1	1.4e-20	86.15	isoflavone 2'-hydroxylase-like [Cucurbita moschata]	[more]
KAG7011035.1	1.4e-20	86.15	Isoflavone 2'-hydroxylase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023512186.1	1.4e-20	86.15	isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_02351...	[more]
XP_022943410.1	1.6e-19	81.54	isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-...	[more]

BLAST of Carg25502 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
W8JMU7	2.6e-09	56.60	Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1	[more]
Q9LVD6	3.8e-08	50.77	Cytochrome P450 81F2 OS=Arabidopsis thaliana OX=3702 GN=CYP81F2 PE=2 SV=1	[more]
Q9FG65	8.4e-08	44.62	Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1	[more]
Q0WTF4	7.1e-07	47.69	Cytochrome P450 81F3 OS=Arabidopsis thaliana OX=3702 GN=CYP81F3 PE=2 SV=1	[more]
Q6WNQ8	2.1e-06	52.54	Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1	[more]

BLAST of Carg25502 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FTX9	6.9e-21	86.15	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=...	[more]
A0A6J1FXS5	7.6e-20	81.54	isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=...	[more]
A0A6J1JDS1	1.1e-18	80.00	isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 ...	[more]
A0A0A0LQI5	1.9e-15	72.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G423640 PE=3 SV=1	[more]
A0A6J1D5S6	9.7e-15	70.77	isoflavone 2'-hydroxylase-like OS=Momordica charantia OX=3673 GN=LOC111017884 PE...	[more]

BLAST of Carg25502 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23190.1	2.4e-10	47.69	cytochrome P450, family 81, subfamily D, polypeptide 7	[more]
AT4G37310.1	9.3e-10	50.00	cytochrome P450, family 81, subfamily H, polypeptide 1	[more]
AT2G23220.1	1.6e-09	46.15	cytochrome P450, family 81, subfamily D, polypeptide 6	[more]
AT5G57220.1	2.7e-09	50.77	cytochrome P450, family 81, subfamily F, polypeptide 2	[more]
AT4G37370.1	4.6e-09	47.69	cytochrome P450, family 81, subfamily D, polypeptide 8	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025724	GAG-pre-integrase domain	PFAM	PF13976	gag_pre-integrs	coord: 96..126 e-value: 1.9E-7 score: 30.8
None	No IPR available	PANTHER	PTHR24281:SF81	CYTOCHROME P450 81E8-LIKE	coord: 3..65
None	No IPR available	PANTHER	PTHR24281	STEROID 21-HYDROXYLASE-RELATED	coord: 3..65

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25502-RA	Carg25502-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen