Carg25500 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25500 vs. NCBI nr
Match: XP_022943410.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata] >KAG7011036.1 Isoflavone 2'-hydroxylase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1005.0 bits (2597), Expect = 2.3e-289
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA
Sbjct: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA
Sbjct: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVYESGN
Sbjct: 481 AKCKPRPIMKLVYESGN 497

BLAST of Carg25500 vs. NCBI nr
Match: XP_022985609.1 (isoflavone 2'-hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 966.5 bits (2497), Expect = 9.1e-278
Identity = 477/497 (95.98%), Postives = 486/497 (97.79%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFLFTLLFA NFVLKPHRL LPP PLLCLPLIGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFASNFVLKPHRLKLPPTPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLV+VISSPSAVQECFTKNDIALANRPLLNSGKYLDYN+TVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVIVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNYTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN FLGVR EEIKCLLCKLCG+ LEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIVSLEIFSTNRLNFFLGVRGEEIKCLLCKLCGNLLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIVM+M+AGKKSFEGHSYKFKEMVT IMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA
Sbjct: 181 FNIVMRMLAGKKSFEGHSYKFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQEL+DEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDT+ 
Sbjct: 241 KTSDELLQELLDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLV 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEWA+SQLLNNPEVLEKARAEIDSSIGYERLVNE+DLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWALSQLLNNPEVLEKARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCA EECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCASEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVYESGN
Sbjct: 481 AKCKPRPIMKLVYESGN 497

BLAST of Carg25500 vs. NCBI nr
Match: XP_022943757.1 (isoflavone 2'-hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 927.5 bits (2396), Expect = 4.7e-266
Identity = 460/497 (92.56%), Postives = 473/497 (95.17%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFAFNFVLKP RL LPPGPL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDI LANRP LNS KYL+YNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLNLFLG+R+EEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIV +MVAGK+ +E H  KF+EMVTQIMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQELVDEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDT+A
Sbjct: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLA 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEW +SQLLN+PEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYACTN 496

BLAST of Carg25500 vs. NCBI nr
Match: XP_023512186.1 (isoflavone 2'-hydroxylase-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023512187.1 isoflavone 2'-hydroxylase-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 922.5 bits (2383), Expect = 1.5e-264
Identity = 452/497 (90.95%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFAFNFVLKPHRLNLPP PL CLP+IGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPHRLNLPPSPLFCLPIIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDI LANRPLLNS KY+DYNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPLLNSAKYIDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLNLFLG+R+EEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIV +MVAGK+ +E H  KF+EMVTQIMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KT DE+L+ELVDEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDT+A
Sbjct: 241 KTCDEILRELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLA 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEWA+SQLLN+PEVLEKARAEID+SIGYERLVNE DLP+LSYVQGIISET+RL+PAAP
Sbjct: 301 VTLEWALSQLLNHPEVLEKARAEIDNSIGYERLVNEVDLPNLSYVQGIISETMRLNPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LL+PHCAFEECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGA+SY
Sbjct: 361 LLVPHCAFEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGADSY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPG+GMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGVGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYACSN 496

BLAST of Carg25500 vs. NCBI nr
Match: XP_023007415.1 (cytochrome P450 81F3-like [Cucurbita maxima])

HSP 1 Score: 764.6 bits (1973), Expect = 5.2e-217
Identity = 375/493 (76.06%), Postives = 431/493 (87.42%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L L +LLFAFNF+  PHRLNLPP PL  LPLIGH HLL HP+HR+L NLSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVV+ISSPSAVQECFTKNDI LANRPLL++GKYL YNHT +++SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFST+RLNLFLG+R+ E+K LL KLCGD LE+ FR VE+EPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEE-FREVEVEPMLSDLT 180

Query: 181 FNIVMKMVAGKKSF-EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKL 240
           FN+VM MV G++ + EG+S KF+++VTQIMAH G++NP DFIPLW ++DPTGF KR   L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 AKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTI 300
           A+  DELLQEL+DE RD +DGGN+MIH LL LQ  EPEYYSD IIKGLIQ I LAGIDT 
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 AVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
           AVTLEWA+S LLNNP+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ INAWA+HRDP+LWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERH--GDSSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 480
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

Query: 481 VAKCKPRPIMKLV 493
           VAKCKPRPIMK +
Sbjct: 481 VAKCKPRPIMKAI 490

BLAST of Carg25500 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.7e-149
Identity = 269/499 (53.91%), Postives = 360/499 (72.14%), Query Frame = 0

Query: 3   ILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKY 62
           ++LLSISL LF         +   R NLPP P L LP+IGHLHL+   +HR+L +LS+KY
Sbjct: 13  VVLLSISLKLFP--------VSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKY 72

Query: 63  GHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYGEH 122
           G VFSL+ G+RLV+V+SSP+A +ECFTKNDI  ANRPL   GKY+ YN+T M  SPYGEH
Sbjct: 73  GSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEH 132

Query: 123 WRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMFN 182
           WRNLRR+ ++EIFS   LN FL +R++E+K LL  L   S +D F  VE++  L +L FN
Sbjct: 133 WRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQD-FGKVEMKSKLSELSFN 192

Query: 183 IVMKMVAGKKSF-----EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGK 242
           + M+MVAGK+ F        +  F+ ++ ++  H+GA+NP DF+P   +ID   + K+  
Sbjct: 193 VTMRMVAGKRYFGQDVDSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVS 252

Query: 243 KLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 302
           K+++  D  LQ L+ E R  ++   TMI  LLSLQ ++PEYY+DQIIKG+I  +LLAG D
Sbjct: 253 KISQEMDAFLQRLIHESRINKN-NVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAGTD 312

Query: 303 TIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHP 362
           T AVT+EWAMS LLN+PE LEKAR EI++ +G  RL+ E DLP L+Y+  IISET RL P
Sbjct: 313 TSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRLCP 372

Query: 363 AAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGA 422
           AAP+L+PH + ++CK++GYDVP+ TI+ +NAWA+HRDP  W++ T FKPERH  G++   
Sbjct: 373 AAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH-GGVE--L 432

Query: 423 ESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 482
           E  KL+PFG+GRR+CPG G+AQRV GLTL +LIQ FEW+R+  + +DM+EG G+TMPKAQ
Sbjct: 433 EPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKAQ 492

Query: 483 PLVAKCKPRPIM-KLVYES 496
           PL A CKPR I+ K+V E+
Sbjct: 493 PLEALCKPRNILHKVVSET 498

BLAST of Carg25500 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 508.8 bits (1309), Expect = 6.8e-143
Identity = 258/504 (51.19%), Postives = 354/504 (70.24%), Query Frame = 0

Query: 1   MDIL-LLSISLFLFTLLFAFNFVLKPHRL-NLPPGPLLCLPLIGHLHLLNHPIHRTLTNL 60
           M+IL LLS S+F   L F FN V++  +  NLPPGP   LP+IG+LH L  P+HRT   L
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGP-PSLPIIGNLHHLKRPLHRTFKGL 60

Query: 61  SQKYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSP 120
           S+KYGHVFSL FGSRLVVV+SS S  Q+CFTKND+ LANRP   SGKY+ YN+T +  + 
Sbjct: 61  SEKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTS 120

Query: 121 YGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLD 180
           YGEHWRNLRRI +L++ S +R+N F G+R++E + L+ +L  DS    F  +EL   L D
Sbjct: 121 YGEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDS-STNFAEMELSSRLYD 180

Query: 181 LMFNIVMKMVAGKKSFE--------GHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPT 240
           + FN +M+M++GK+ +           + +F++MV++++  SGA N  DF+PL  F+D  
Sbjct: 181 MTFNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFE 240

Query: 241 GFGKRGKKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQD 300
              KR K ++  +D  L+ L++E R K++  NTMI  LL+LQ+++PEYY+DQIIKGL   
Sbjct: 241 NLEKRLKDISGKTDAFLRGLIEEHRTKKERANTMIDHLLNLQDSQPEYYTDQIIKGLALA 300

Query: 301 ILLAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 360
           +LLAG D+ AVTLEW+MS LLN+PEVL+K + E+D+ +G +RLV+ESDLP L+Y++ +I+
Sbjct: 301 MLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVIN 360

Query: 361 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 420
           ETLRL+  APLLLPH   +EC I GY VP+DTI+ INAWA+HRDP LW +AT+FKPER  
Sbjct: 361 ETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPER-- 420

Query: 421 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 480
              +   E  KLI FG+GRRACPG G+A R   +TLA LIQ F+W+ +N   +D++E  G
Sbjct: 421 --FEKKGELEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDG 480

Query: 481 ITMPKAQPLVAKCKPRPIMKLVYE 495
            T+ K  PL A CK RP++  V++
Sbjct: 481 FTLTKLVPLKAMCKSRPVINKVFK 498

BLAST of Carg25500 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 494.2 bits (1271), Expect = 1.7e-138
Identity = 259/496 (52.22%), Postives = 339/496 (68.35%), Query Frame = 0

Query: 5   LLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKYGH 64
           LLS+S F+ T+        +  + NLPPGP   +P+IG+LH L HP+HRT T LSQ YG 
Sbjct: 7   LLSLS-FIITIKILLKITSRRLK-NLPPGP-PTIPIIGNLHHLKHPLHRTFTTLSQTYGD 66

Query: 65  VFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYGEHWR 124
           +FSL FGSRLVVV+SSPS   ECFTKNDI LANRP   +GKY+ YN+T +  + YG+HWR
Sbjct: 67  IFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWR 126

Query: 125 NLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLED--RFRVVELEPMLLDLMFN 184
           NLRRI ++++ S NRLN FLGVR++E   L+ KL  D + +   F  VEL P L ++ FN
Sbjct: 127 NLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFN 186

Query: 185 IVMKMVAGKKSF--EG------HSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGK 244
            +M+M++GK+ +  +G       + +F+E+++++M+  GA N  DF+PL   +D     K
Sbjct: 187 AMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEK 246

Query: 245 RGKKLAKTSDELLQELVDEIR--DKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDIL 304
           R K++AK S+  L+ L++E R  +    G TMI  LL L  ++PEYYSD +IKGLIQ +L
Sbjct: 247 RCKRIAKRSNAFLEGLIEEHRRGNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGML 306

Query: 305 LAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISET 364
           LAG DT AVT+EW MS+LLN+PEVL+KA+ E+D+ IG  +LV+E DL  L Y+Q IISET
Sbjct: 307 LAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISET 366

Query: 365 LRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANG 424
           LRLHP APLLLPH + E+C I  ++VP+DTII  N W +HRDP  W DA SFKPER    
Sbjct: 367 LRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPER---- 426

Query: 425 MKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGIT 484
            +   E  K++ FGLGRRACPGL +AQR  G T+  LIQ FEWER +   +DM EGKGIT
Sbjct: 427 FEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGIT 486

Query: 485 MPKAQPLVAKCKPRPI 489
           MP   PL A CK  PI
Sbjct: 487 MPMKIPLRAMCKALPI 495

BLAST of Carg25500 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 485.7 bits (1249), Expect = 6.1e-136
Identity = 247/498 (49.60%), Postives = 338/498 (67.87%), Query Frame = 0

Query: 6   LSISLFLFTLLFAFNFVLKPHRL-NLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKYGH 65
           L  SLF  ++ F    + +  +  NLPPGP   LP+IG+LH L  P++RT   L++KYG+
Sbjct: 7   LCYSLFYLSIFFIIRLLFQSRKFKNLPPGP-TSLPIIGNLHHLKRPLNRTFKALTEKYGN 66

Query: 66  VFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYGEHWR 125
           V SL FGSRLVVV+SS S  QECFTKND+ LANRP   SGKY+ YN+T +  + YGEHWR
Sbjct: 67  VISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWR 126

Query: 126 NLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMFNIV 185
           NLRRI SL++ S +R+N F  +R++E + L+ KL  DS   +F  VEL     D+ FN +
Sbjct: 127 NLRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDS-STKFAEVELTFRFFDMTFNNI 186

Query: 186 MKMVAGKKSFEG--------HSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRG 245
           M+M++GK+ +           + +F++MV++++  SGA N  DF+PL  F+D     KR 
Sbjct: 187 MRMISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRV 246

Query: 246 KKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGI 305
           K++ + +D  L  L+ E R K++  NTMI  LL++Q ++PEYY+D IIKGL   +LLAG 
Sbjct: 247 KRIGEKNDVFLSGLLQEQRSKKERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAGT 306

Query: 306 DTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLH 365
           D+ AVTLEW MS +LN PEVL+K R E+D+ +G +RLV+ESDLP L+Y++ +I ETLRL+
Sbjct: 307 DSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRLY 366

Query: 366 PAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNG 425
             APLLLPH   +EC + GY VPRDTI+ INAWA+HRDP  W +AT+FKPER        
Sbjct: 367 TPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPER----FDKK 426

Query: 426 AESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKA 485
            E  K+I FG+GRRACPG G+A R   +TLA L+Q F+W+R+N+  +DMSE  G TM K 
Sbjct: 427 GELEKMIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERDGFTMTKL 486

Query: 486 QPLVAKCKPRPIMKLVYE 495
            PL A CK RP++  V++
Sbjct: 487 LPLKAMCKTRPVVNKVFK 498

BLAST of Carg25500 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 481.9 bits (1239), Expect = 8.9e-135
Identity = 253/499 (50.70%), Postives = 345/499 (69.14%), Query Frame = 0

Query: 3   ILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKY 62
           ++L SI   +F L+ +F F LKP + NLPP P   LP+IGHL LL  PIHRTL + S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----VFSLRFGSRLVVVISSPS-AVQECFTKNDIALANRPLLNSGKYLDYNHTVMSV 122
            H     V SLR GSRLV V+SS   A +ECF KND+ LANRP +  GK++ YN+T M  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPML 182
           +PYG+HWRNLRR+ ++EIFST+RLN FL VR +E++ L+ +L       +  VVEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPML 187

Query: 183 LDLMFNIVMKMVAGKKSF------EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPT 242
           +DL FN +M+M+ GK+ +      E  + + +++V  + A++ + N  D++P+      +
Sbjct: 188 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 247

Query: 243 GFGKRGKKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQD 302
            +  R KKL + +D+ LQ L+D+ R +++ G TMI  LL LQ ++ EYY+DQIIKG+I  
Sbjct: 248 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307

Query: 303 ILLAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 362
           +++AG +T AVTLEWA+S LLN+P+V+ KAR EID+ +G +RL+ E+DL  L Y++ I+ 
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 367

Query: 363 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 422
           ETLRLHPA PLL+PH A E+CKI  YD+PR T + +NAWA+HRDPN W+D  SFKPER  
Sbjct: 368 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER-- 427

Query: 423 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 482
              +   E+ KL+ FGLGRRACPG G+AQR+ GL L SLIQ FEWER+ N  VDM EG G
Sbjct: 428 --FEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487

Query: 483 ITMPKAQPLVAKCKPRPIM 490
            T+PKA PL A CK RP +
Sbjct: 488 NTVPKAIPLKAICKARPFL 497

BLAST of Carg25500 vs. ExPASy TrEMBL
Match: A0A6J1FXS5 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448183 PE=3 SV=1)

HSP 1 Score: 1005.0 bits (2597), Expect = 1.1e-289
Identity = 497/497 (100.00%), Postives = 497/497 (100.00%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA
Sbjct: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA
Sbjct: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVYESGN
Sbjct: 481 AKCKPRPIMKLVYESGN 497

BLAST of Carg25500 vs. ExPASy TrEMBL
Match: A0A6J1JDS1 (isoflavone 2'-hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111483622 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.4e-278
Identity = 477/497 (95.98%), Postives = 486/497 (97.79%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLFLFTLLFA NFVLKPHRL LPP PLLCLPLIGHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFLFTLLFASNFVLKPHRLKLPPTPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLV+VISSPSAVQECFTKNDIALANRPLLNSGKYLDYN+TVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVIVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNYTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN FLGVR EEIKCLLCKLCG+ LEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRIVSLEIFSTNRLNFFLGVRGEEIKCLLCKLCGNLLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIVM+M+AGKKSFEGHSYKFKEMVT IMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA
Sbjct: 181 FNIVMRMLAGKKSFEGHSYKFKEMVTHIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQEL+DEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDT+ 
Sbjct: 241 KTSDELLQELLDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLV 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEWA+SQLLNNPEVLEKARAEIDSSIGYERLVNE+DLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWALSQLLNNPEVLEKARAEIDSSIGYERLVNEADLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCA EECKIEGYDVPRDTII INAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCASEECKIEGYDVPRDTIILINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVYESGN
Sbjct: 481 AKCKPRPIMKLVYESGN 497

BLAST of Carg25500 vs. ExPASy TrEMBL
Match: A0A6J1FTX9 (isoflavone 2'-hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111448413 PE=3 SV=1)

HSP 1 Score: 927.5 bits (2396), Expect = 2.3e-266
Identity = 460/497 (92.56%), Postives = 473/497 (95.17%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSISLF+F LLFAFNFVLKP RL LPPGPL  LPL+GHLHLLNHPIHRTLTNLSQ
Sbjct: 1   MDILLLSISLFIFPLLFAFNFVLKPRRLKLPPGPLFSLPLLGHLHLLNHPIHRTLTNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDI LANRP LNS KYL+YNHTVMSVSPYG
Sbjct: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIVLANRPFLNSTKYLNYNHTVMSVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLNLFLG+R+EEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM
Sbjct: 121 EHWRNLRRISSLEIFSTNRLNLFLGIREEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180

Query: 181 FNIVMKMVAGKKSFEGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKLA 240
           FNIV +MVAGK+ +E H  KF+EMVTQIMAHSGATNP DFIPLW FIDP+GF KR KKL 
Sbjct: 181 FNIVTRMVAGKRFYERHD-KFREMVTQIMAHSGATNPGDFIPLWRFIDPSGFEKRVKKLG 240

Query: 241 KTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTIA 300
           KTSDELLQELVDEIRDK DGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDT+A
Sbjct: 241 KTSDELLQELVDEIRDKNDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTLA 300

Query: 301 VTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360
           VTLEW +SQLLN+PEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP
Sbjct: 301 VTLEWTVSQLLNHPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAAP 360

Query: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420
           LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY
Sbjct: 361 LLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAESY 420

Query: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480
           KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV
Sbjct: 421 KLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPLV 480

Query: 481 AKCKPRPIMKLVYESGN 498
           AKCKPRPIMKLVY   N
Sbjct: 481 AKCKPRPIMKLVYACTN 496

BLAST of Carg25500 vs. ExPASy TrEMBL
Match: A0A6J1L2W8 (cytochrome P450 81F3-like OS=Cucurbita maxima OX=3661 GN=LOC111499919 PE=3 SV=1)

HSP 1 Score: 764.6 bits (1973), Expect = 2.5e-217
Identity = 375/493 (76.06%), Postives = 431/493 (87.42%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L L +LLFAFNF+  PHRLNLPP PL  LPLIGH HLL HP+HR+L NLSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHNLSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVV+ISSPSAVQECFTKNDI LANRPLL++GKYL YNHT +++SPYG
Sbjct: 61  KHGHVISLRFGSRLVVLISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAISPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFST+RLNLFLG+R+ E+K LL KLCGD LE+ FR VE+EPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTSRLNLFLGIRQNEVKRLLRKLCGDWLEE-FREVEVEPMLSDLT 180

Query: 181 FNIVMKMVAGKKSF-EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKL 240
           FN+VM MV G++ + EG+S KF+++VTQIMAH G++NP DFIPLW ++DPTGF KR   L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 AKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTI 300
           A+  DELLQEL+DE RD +DGGN+MIH LL LQ  EPEYYSD IIKGLIQ I LAGIDT 
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNSMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 AVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
           AVTLEWA+S LLNNP+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLRYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ INAWA+HRDP+LWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINAWAIHRDPDLWEDPTAFKPERH--GDSSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 480
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

Query: 481 VAKCKPRPIMKLV 493
           VAKCKPRPIMK +
Sbjct: 481 VAKCKPRPIMKAI 490

BLAST of Carg25500 vs. ExPASy TrEMBL
Match: A0A6J1G5P7 (cytochrome P450 81F3 OS=Cucurbita moschata OX=3662 GN=LOC111451033 PE=3 SV=1)

HSP 1 Score: 763.8 bits (1971), Expect = 4.3e-217
Identity = 376/493 (76.27%), Postives = 428/493 (86.82%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQ 60
           MDILLLSI L L +LLFAFNF+  PHRLNLPP PL  LPLIGH HLL HP+HR+L  LSQ
Sbjct: 1   MDILLLSIPLLLLSLLFAFNFLQTPHRLNLPPTPLFSLPLIGHFHLLKHPLHRSLHALSQ 60

Query: 61  KYGHVFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 120
           K+GHV SLRFGSRLVVVISSPSAVQECFTKNDI LANRPLL++GKYL YNHT ++VSPYG
Sbjct: 61  KHGHVISLRFGSRLVVVISSPSAVQECFTKNDIILANRPLLDTGKYLAYNHTTLAVSPYG 120

Query: 121 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 180
           EHWRNLRRI SLEIFSTNRLN+F+G+R+ E+K LL KLCGD  E+ FR VELEPML DL 
Sbjct: 121 EHWRNLRRICSLEIFSTNRLNMFVGIRENEVKRLLRKLCGDWSEE-FREVELEPMLSDLT 180

Query: 181 FNIVMKMVAGKKSF-EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGKKL 240
           FN+VM MV G++ + EG+S KF+++VTQIMAH G++NP DFIPLW ++DPTGF KR   L
Sbjct: 181 FNVVMGMVGGRRFYDEGYSKKFRDLVTQIMAHGGSSNPGDFIPLWNWVDPTGFKKRIMNL 240

Query: 241 AKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGIDTI 300
           A+  DELLQEL+DE RD +DGGNTMIH LL LQ  EPEYYSD IIKGLIQ I LAGIDT 
Sbjct: 241 ARRGDELLQELIDEARDGKDGGNTMIHHLLCLQKDEPEYYSDLIIKGLIQVIFLAGIDTS 300

Query: 301 AVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHPAA 360
           AVTLEWA+S LLNNP+VL+KARAEID  IG ERLVNESDLP+L Y+QGIISETLRL+PAA
Sbjct: 301 AVTLEWALSHLLNNPDVLKKARAEIDDLIGKERLVNESDLPNLKYLQGIISETLRLNPAA 360

Query: 361 PLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGAES 420
           PLL+PHCAF++CKIEGYDVPRDTI+ IN WA+HRDPNLWED T+FKPERH  G  +GA+S
Sbjct: 361 PLLVPHCAFQDCKIEGYDVPRDTIVLINTWAIHRDPNLWEDPTAFKPERH--GDTSGADS 420

Query: 421 YKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQPL 480
           YKL+PFGLGRRACPG+GMAQRV GL LASLIQGFEWER+++SLVDMSEG+G+TMPKAQPL
Sbjct: 421 YKLLPFGLGRRACPGVGMAQRVVGLALASLIQGFEWERVSSSLVDMSEGQGLTMPKAQPL 480

Query: 481 VAKCKPRPIMKLV 493
           VAKCKPRPIMK +
Sbjct: 481 VAKCKPRPIMKAI 490

BLAST of Carg25500 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 490.7 bits (1262), Expect = 1.4e-138
Identity = 254/501 (50.70%), Postives = 352/501 (70.26%), Query Frame = 0

Query: 4   LLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKYG 63
           L+ SI   + +L++    + +  + NLPP P   LP+IGHL LL  PIHRT  +LSQ   
Sbjct: 6   LIFSILFVVLSLIYLIGKLKR--KPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLN 65

Query: 64  H--VFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYGE 123
           +  +FSLR G+RLV V SS S  +ECFTKND+ LANRP     K++ Y++T M  + YG+
Sbjct: 66  NAPIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGD 125

Query: 124 HWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLMF 183
           HWRNLRRIGS+EIFS +RLN FL +RK+EI+ L+ +L   +    F  V+++ ML DL F
Sbjct: 126 HWRNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRL-SRNFSQEFVKVDMKSMLSDLTF 185

Query: 184 NIVMKMVAGKKSF------EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKR 243
           N +++MVAGK+ +      +  + + ++++  ++A +GA N  D++P+   +  + +  R
Sbjct: 186 NNILRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETR 245

Query: 244 GKKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAG 303
            KKLA   DE LQ LVDE R+ ++ GNTMI  LL+LQ ++P+Y++D+IIKG +  ++LAG
Sbjct: 246 VKKLAGRLDEFLQGLVDEKREAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 305

Query: 304 IDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRL 363
            DT AVTLEWA+S +LN+P+VL KAR EID  IG +RL++ESD+ +L Y+Q I+SETLRL
Sbjct: 306 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 365

Query: 364 HPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKN 423
           +PAAP+LLPH A E+CK+ GYD+PR TI+  N WA+HRDP LW+D  SFKPER     + 
Sbjct: 366 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPER----FEK 425

Query: 424 GAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPK 483
             E+ KL+PFGLGRRACPG G+A R+  LTL SLIQ  EWE++    VDMSEGKG+TMPK
Sbjct: 426 EGEAQKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGEE-VDMSEGKGVTMPK 485

Query: 484 AQPLVAKCKPRP-IMKLVYES 496
           A+PL A C+ RP ++K+  ES
Sbjct: 486 AKPLEAMCRARPSVVKIFNES 496

BLAST of Carg25500 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 490.3 bits (1261), Expect = 1.8e-138
Identity = 256/498 (51.41%), Postives = 343/498 (68.88%), Query Frame = 0

Query: 1   MDILLLSISLFLFTLLFAFNFVLKPHRL-NLPPGPLLCLPLIGHLHLLNHPIHRTLTNLS 60
           MD+  + +  FLF  +     + K +R  NLPP P   LP+IGHLHLL  P+HRT  ++S
Sbjct: 1   MDLTQILLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSIS 60

Query: 61  QKYGH--VFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVS 120
           +  G+  +F LR G+RLV VISS S  +ECFTKND+ LANRP +   K++ YN T M  +
Sbjct: 61  KSLGNAPIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAA 120

Query: 121 PYGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLL 180
            YG+HWRNLRRI ++EIFS++R++ F  +RK+EI+ L+  L  DSL   F  VEL+ +L 
Sbjct: 121 SYGDHWRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHG-FVEVELKSLLT 180

Query: 181 DLMFNIVMKMVAGKKSF------EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTG 240
           +L FN ++ MVAGK+ +         +   +E++ +IMA +G+ N AD++P   ++  T 
Sbjct: 181 NLAFNNIIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TN 240

Query: 241 FGKRGKKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDI 300
           F  + K L    D +LQ+LVDE R +++ G T+I  LLS Q TEPEYY+D IIKG+I  +
Sbjct: 241 FENQTKILGNRLDRVLQKLVDEKRAEKEKGQTLIDHLLSFQETEPEYYTDVIIKGIILAL 300

Query: 301 LLAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISE 360
           +LAG DT +VTLEWAMS LLN+PE+LEKARAEID  IG +RLV ESD+ +L Y+Q I+SE
Sbjct: 301 VLAGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSE 360

Query: 361 TLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHAN 420
           TLRL+PA PLLLPH + +ECK+ GYD+PR T++  N WAMHRDP LWE+   FKPER   
Sbjct: 361 TLRLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPER--- 420

Query: 421 GMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGI 480
             +   E+ KL+PFG+GRRACPG  + +R+  L L  LIQ FEWER+   LVDM+EG+GI
Sbjct: 421 -FEKEGEARKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGI 480

Query: 481 TMPKAQPLVAKCKPRPIM 490
           TMPKA PL A CK R I+
Sbjct: 481 TMPKATPLRAMCKARAIV 491

BLAST of Carg25500 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 483.4 bits (1243), Expect = 2.2e-136
Identity = 253/492 (51.42%), Postives = 339/492 (68.90%), Query Frame = 0

Query: 3   ILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKY 62
           IL+LS  L LFTL   F       +LNLPP P + LP+IGHLHLL  P+HRT  +LS+  
Sbjct: 6   ILILSF-LSLFTLAI-FLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSI 65

Query: 63  GH--VFSLRFGSRLVVVISSPSAVQECFTKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 122
           G+  VF LR G+RLV VISS S  +ECFTKND+ LANRP     K+L YN T +  + YG
Sbjct: 66  GNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYG 125

Query: 123 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 182
           +HWRNLRRI ++EIFST+RLN FL +RK+EI+ L+  L  DSL   F  VE++ +L +L 
Sbjct: 126 DHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHG-FVEVEMKTLLTNLA 185

Query: 183 FNIVMKMVAGKKSF---EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFGKRGK 242
            N  ++M+AGK+ F      +   K +V++ +  +GA NP D++ +  ++  + + KR K
Sbjct: 186 SNTTIRMLAGKRYFGEDNDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIK 245

Query: 243 KLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQDILLAGID 302
            L    D  LQ+LVDE R +++ G TMI  LL+LQ+ +P+YY+D IIKG+I  +++AG D
Sbjct: 246 NLGNRFDTFLQKLVDEKRAEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGTD 305

Query: 303 TIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIISETLRLHP 362
           T +VTLEWAMS LLN+PE+L+KAR EID  +G +RLV+ESD+ +LSY+Q I+ ETLR++P
Sbjct: 306 TSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMYP 365

Query: 363 AAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHANGMKNGA 422
           A PLLLPH + E+CK+ GYD+P  T++  NAWAMHRDP +WED   FKPER     +   
Sbjct: 366 AVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPER----FEKEG 425

Query: 423 ESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKGITMPKAQ 482
           E+ KLI FG+GRRACPG G+A R+    L SL+Q FEWER+    VDM+E KG T+PKA 
Sbjct: 426 EAEKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAI 485

Query: 483 PLVAKCKPRPIM 490
           PL A CK R I+
Sbjct: 486 PLRAMCKARSIV 488

BLAST of Carg25500 vs. TAIR 10
Match: AT4G37310.1 (cytochrome P450, family 81, subfamily H, polypeptide 1 )

HSP 1 Score: 482.6 bits (1241), Expect = 3.7e-136
Identity = 253/499 (50.70%), Postives = 340/499 (68.14%), Query Frame = 0

Query: 3   ILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHP-IHRTLTNLSQK 62
           ILL+  +L    ++     V KP++ NLPP P +C P+IGHLHLL  P +HRTL++LS  
Sbjct: 4   ILLILTTLVAIFIVKIVLLVTKPNK-NLPPSPNICFPIIGHLHLLKKPLLHRTLSHLSHS 63

Query: 63  YGHVFSLRFGSRLVVVISSPSAVQECF-TKNDIALANRPLLNSGKYLDYNHTVMSVSPYG 122
            G VFSLR GSRL V+ISSP+A +ECF TKNDI LANRP    GKY+ Y++T M  +PYG
Sbjct: 64  LGPVFSLRLGSRLAVIISSPTAAEECFLTKNDIVLANRPRFIMGKYVAYDYTSMVTAPYG 123

Query: 123 EHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPMLLDLM 182
           +HWRNLRRI +LE+FSTNRLN    +R +E+K LL KL   S+E R   VEL  +L  L 
Sbjct: 124 DHWRNLRRITALEVFSTNRLNASAEIRHDEVKMLLQKLHDLSVE-RPAKVELRQLLTGLT 183

Query: 183 FNIVMKMVAGKKSFE-------GHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPTGFG 242
            N++M+M+ GK+ FE       G S +F+E+V +I+  S A NPADF+P   + D  G  
Sbjct: 184 LNVIMRMMTGKRFFEEDDGGKAGISLEFRELVAEILELSAADNPADFLPALRWFDYKGLV 243

Query: 243 KRGKKLAKTSDELLQELVDEIR---DKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQD 302
           KR K++ +  D LLQ  +DE R   D+ +  NTMI  LL  Q  EP  YSDQ IKGLI  
Sbjct: 244 KRAKRIGERMDSLLQGFLDEHRANKDRLEFKNTMIAHLLDSQEKEPHNYSDQTIKGLILM 303

Query: 303 ILLAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSI---GYERLVNESDLPSLSYVQG 362
           +++ G DT A+T+EWAMS LLN+P++LE  R  ID+ +      RL+ E DL +++Y++ 
Sbjct: 304 MVVGGTDTSALTVEWAMSNLLNHPQILETTRQNIDTQMETSSSRRLLKEEDLVNMNYLKN 363

Query: 363 IISETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPE 422
           ++SETLRL+P APL++PH    +C I G++VPRDTI+ +N WA+HRDP++W+D TSFKPE
Sbjct: 364 VVSETLRLYPVAPLMVPHVPSSDCVIGGFNVPRDTIVLVNLWAIHRDPSVWDDPTSFKPE 423

Query: 423 RHANGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSE 482
           R     + G  + K++PFGLGRRACPGL +A RV GL L S+IQ FEWE  +   VDM+E
Sbjct: 424 RFEGSDQFGHYNGKMMPFGLGRRACPGLSLANRVVGLLLGSMIQCFEWESGSGGQVDMTE 483

Query: 483 GKGITMPKAQPLVAKCKPR 487
           G G+++PKA+PLV  C+ R
Sbjct: 484 GPGLSLPKAEPLVVTCRTR 500

BLAST of Carg25500 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 481.9 bits (1239), Expect = 6.3e-136
Identity = 253/499 (50.70%), Postives = 345/499 (69.14%), Query Frame = 0

Query: 3   ILLLSISLFLFTLLFAFNFVLKPHRLNLPPGPLLCLPLIGHLHLLNHPIHRTLTNLSQKY 62
           ++L SI   +F L+ +F F LKP + NLPP P   LP+IGHL LL  PIHRTL + S+  
Sbjct: 8   VVLYSIFSLIF-LIISFKF-LKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETL 67

Query: 63  GH-----VFSLRFGSRLVVVISSPS-AVQECFTKNDIALANRPLLNSGKYLDYNHTVMSV 122
            H     V SLR GSRLV V+SS   A +ECF KND+ LANRP +  GK++ YN+T M  
Sbjct: 68  DHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIA 127

Query: 123 SPYGEHWRNLRRIGSLEIFSTNRLNLFLGVRKEEIKCLLCKLCGDSLEDRFRVVELEPML 182
           +PYG+HWRNLRR+ ++EIFST+RLN FL VR +E++ L+ +L       +  VVEL+PML
Sbjct: 128 APYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRL-SRLAGTKKTVVELKPML 187

Query: 183 LDLMFNIVMKMVAGKKSF------EGHSYKFKEMVTQIMAHSGATNPADFIPLWGFIDPT 242
           +DL FN +M+M+ GK+ +      E  + + +++V  + A++ + N  D++P+      +
Sbjct: 188 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLF--S 247

Query: 243 GFGKRGKKLAKTSDELLQELVDEIRDKEDGGNTMIHRLLSLQNTEPEYYSDQIIKGLIQD 302
            +  R KKL + +D+ LQ L+D+ R +++ G TMI  LL LQ ++ EYY+DQIIKG+I  
Sbjct: 248 SYENRVKKLGEETDKFLQGLIDDKRGQQETGTTMIDHLLVLQKSDIEYYTDQIIKGIILI 307

Query: 303 ILLAGIDTIAVTLEWAMSQLLNNPEVLEKARAEIDSSIGYERLVNESDLPSLSYVQGIIS 362
           +++AG +T AVTLEWA+S LLN+P+V+ KAR EID+ +G +RL+ E+DL  L Y++ I+ 
Sbjct: 308 MVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVL 367

Query: 363 ETLRLHPAAPLLLPHCAFEECKIEGYDVPRDTIIFINAWAMHRDPNLWEDATSFKPERHA 422
           ETLRLHPA PLL+PH A E+CKI  YD+PR T + +NAWA+HRDPN W+D  SFKPER  
Sbjct: 368 ETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER-- 427

Query: 423 NGMKNGAESYKLIPFGLGRRACPGLGMAQRVAGLTLASLIQGFEWERLNNSLVDMSEGKG 482
              +   E+ KL+ FGLGRRACPG G+AQR+ GL L SLIQ FEWER+ N  VDM EG G
Sbjct: 428 --FEKEEEAQKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVG 487

Query: 483 ITMPKAQPLVAKCKPRPIM 490
            T+PKA PL A CK RP +
Sbjct: 488 NTVPKAIPLKAICKARPFL 497