Carg25494 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25494 vs. NCBI nr
Match: XP_022944057.1 (cytochrome P450 81E8-like [Cucurbita moschata] >KAG7011030.1 Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 996.5 bits (2575), Expect = 8.2e-287
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
           TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW
Sbjct: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL
Sbjct: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMCRALPIMDKVLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Carg25494 vs. NCBI nr
Match: XP_023512643.1 (cytochrome P450 81E8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965.7 bits (2495), Expect = 1.6e-277
Identity = 482/499 (96.59%), Postives = 492/499 (98.60%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL VLLLLLLVFLFLQQFIT RRNLPPSPPS PIIGHLHLLQRPIHRNFH+IAAKYGP
Sbjct: 1   MDFLSVLLLLLLVFLFLQQFITCRRNLPPSPPSVPIIGHLHLLQRPIHRNFHHIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTP+GDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFA+VELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFARVELKSMFSELSFNVVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
           TGKRYFGEEVSNETEAREFRELMDETSRHGGASNW+DFMPILKWIGFGEYEKSLAKLTKR
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWIDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRL+DEADL NLHYLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDEADLTNLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DCTVAGYD+PRGTMLLVNAWAIHRDPKLWDDPM FRPERFLGEGNELL 
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLLVNAWAIHRDPKLWDDPMRFRPERFLGEGNELLS 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFGMGRRACPGETMA RLIGLSLGLLIQCY+WKNVGY+KVDMTEGGGITIQKVQPL
Sbjct: 421 NKLIPFGMGRRACPGETMAQRLIGLSLGLLIQCYDWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ET+CRALPIMDKVLSNGLD
Sbjct: 481 ETLCRALPIMDKVLSNGLD 499

BLAST of Carg25494 vs. NCBI nr
Match: XP_022985607.1 (cytochrome P450 81E8-like [Cucurbita maxima] >XP_022985608.1 cytochrome P450 81E8-like [Cucurbita maxima])

HSP 1 Score: 950.7 bits (2456), Expect = 5.2e-273
Identity = 473/499 (94.79%), Postives = 487/499 (97.60%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL V LLLLLVFLFLQQFITRRRNLPPSPPS PIIGHLHLLQRPIH+NFHNIAAKYGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTP+GD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRLTSME+FSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
           TGKRYFGEEVSNETEAREFRELMDE SRHGGASNW++FMPILKWIGFGEYEKSLAKLTKR
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           ADKFMQKLVE RRNQTG +IEKQSTLLDRLLELQASEPEYYSDEIIKGV+LVLLRAG DS
Sbjct: 241 ADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAGVDS 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRL+DE+DLANLHYLQAII ETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRLHPA 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DCTVAGYD+PRGTML+VNAWAIHRDPKLWDDPMSFRPERFLG GNELL 
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNELLS 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKL+PFGMGRRACPGETMA RLIGLSLGLLIQCYEWKNVGY+KVDMTEGGGITIQKVQPL
Sbjct: 421 NKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMCRALPIMDKVLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Carg25494 vs. NCBI nr
Match: XP_038902132.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 791.6 bits (2043), Expect = 4.0e-225
Identity = 386/499 (77.35%), Postives = 444/499 (88.98%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL  +LLL L F+FL Q  TRRRNLPPSPPS PIIGHLHLLQRPIHRNF NIAAKYGP
Sbjct: 1   MDFLSAVLLLCLAFVFLLQLRTRRRNLPPSPPSAPIIGHLHLLQRPIHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQ+A+ECFTK+D++FANRPR+L GKYLGYN+TT++T  +GDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQIAQECFTKHDLIFANRPRMLSGKYLGYNYTTMATASYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           +LRRLT+ EIFS  RLNA+L IRKDEIRRLL+KLHSESFAKVEL+SMFSELSFN+VMR+V
Sbjct: 121 SLRRLTATEIFSPTRLNASLGIRKDEIRRLLLKLHSESFAKVELRSMFSELSFNIVMRIV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
            GKRY+GE+VS+E EAREF ELM++ SRHGGAS WVDFMPILKW GFG YEKSLAK    
Sbjct: 181 AGKRYYGEKVSDEAEAREFIELMEDVSRHGGASQWVDFMPILKWFGFGGYEKSLAKSAIW 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           AD+F+Q+LVE+RRNQ  L  E+QS+LL RLLELQ SEPE ++D+IIKG++LVLLRAG D+
Sbjct: 241 ADRFVQELVEERRNQKVLGREEQSSLLHRLLELQLSEPESHTDQIIKGIVLVLLRAGIDT 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           +SVT++W MT+LLN+P V++KAKAEIDTKIG+DRL++E D+ANL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWIMTELLNHPGVLAKAKAEIDTKIGQDRLVEETDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLL HYSS DC VAGY++P GTMLLVNAWAIHRDPKLWDDP SFRPERFLG GNEL  
Sbjct: 361 APMLLAHYSSADCIVAGYNIPPGTMLLVNAWAIHRDPKLWDDPTSFRPERFLGVGNELQA 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFG+GRRACPGE M LR++GL+LGLLIQCYEWKNV  E VDM+E GGITI KV+PL
Sbjct: 421 NKLIPFGVGRRACPGEIMGLRVVGLTLGLLIQCYEWKNVECENVDMSESGGITILKVKPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMC++ PIMDKVLSNGLD
Sbjct: 481 ETMCKSRPIMDKVLSNGLD 499

BLAST of Carg25494 vs. NCBI nr
Match: XP_008455812.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo] >KAA0032504.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 784.6 bits (2025), Expect = 4.9e-223
Identity = 382/499 (76.55%), Postives = 439/499 (87.98%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL    LL L FL LQQ  TRRRNLPPSPPSFPIIGHLHLLQRP HRNF NIAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IF+LRFGSRLA+IVSSLQ+A+ECFTK+D++FANRPRLL GKY GY++TT++T  +GDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRL +MEIFS  RLNA+L+ RKDEIRRLLVKLHSESFAKVEL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
            GKRY+GE+VS+E EAREFRELMDE SR GG S WVDFMPILKWIGFG YEK L K    
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           AD+F+Q+L+E+ RN+  L  E+QS+LL RLLELQ S+PEYY+D+IIKG++LVLLRAG D+
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           +SVT++WAMT+LLN+PEV++KAKAEIDTKIG+DR ++E+D+ANL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DC VAGY++PRGTMLLVNA AIHRDPKLWDDP SFRPERFLG GNEL  
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFG+GRRACPGE M LR++GL+LGLLIQCYEWK VGYE VD TE GGITI KV+P+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMC+  P+M KVLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Carg25494 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.4e-159
Identity = 277/498 (55.62%), Postives = 377/498 (75.70%), Query Frame = 0

Query: 3   FLFVLLLLLLVFLFLQQFITRRRNLPPSPP-SFPIIGHLHLLQRPIHRNFHNIAAKYGPI 62
           + F+ ++LL + L L     RRRNLPPSP  + P+IGHLHL+ + +HR+ ++++ KYG +
Sbjct: 8   YTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDLSKKYGSV 67

Query: 63  FTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWRN 122
           F+L+ G+RL L+VSS   AEECFTKND+VFANRP  ++GKY+GYN+TT+  +P+G+HWRN
Sbjct: 68  FSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSPYGEHWRN 127

Query: 123 LRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVMR 182
           LRRL ++EIFS   LN  LSIR+DE+++LL+ L+  S   F KVE+KS  SELSFNV MR
Sbjct: 128 LRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSELSFNVTMR 187

Query: 183 VVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLT 242
           +V GKRYFG++V ++ EA+ FR L+ E   H GASN  DF+P L+WI F  YEK ++K++
Sbjct: 188 MVAGKRYFGQDVDSD-EAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYEKKVSKIS 247

Query: 243 KRADKFMQKLVEDRRNQTGLQIEKQS-TLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 302
           +  D F+Q+L+ + R      I K + T++D LL LQ S+PEYY+D+IIKG+I+VLL AG
Sbjct: 248 QEMDAFLQRLIHESR------INKNNVTMIDHLLSLQESQPEYYTDQIIKGIIMVLLLAG 307

Query: 303 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 362
            D+++VT+EWAM+ LLN+PE + KA+ EI+T++G +RLI+E DL  L YL  IISETFRL
Sbjct: 308 TDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIISETFRL 367

Query: 363 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 422
            PAAPML+ H SS+DC V GYDVP+GT+LLVNAWAIHRDP+ WD+P  F+PER    G E
Sbjct: 368 CPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPERH--GGVE 427

Query: 423 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 482
           L  +KL+PFGMGRR+CPG  +A R++GL+LG LIQC+EWK +G  K+DM EG G+T+ K 
Sbjct: 428 LEPSKLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKIDMAEGSGLTMPKA 487

Query: 483 QPLETMCRALPIMDKVLS 496
           QPLE +C+   I+ KV+S
Sbjct: 488 QPLEALCKPRNILHKVVS 496

BLAST of Carg25494 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.9e-149
Identity = 263/489 (53.78%), Postives = 353/489 (72.19%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRR--RNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGPI 63
           LF L++ L VF     F T R  +NLPP P   PIIG+LH L++P+H  FH ++ KYG I
Sbjct: 12  LFFLIITLKVF-----FNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYGQI 71

Query: 64  FTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWRN 123
           F+L FGSRL ++VSSL +A+ECFTKND+V ANRP  L GKY+GYN TT++ +P+GDHWRN
Sbjct: 72  FSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRN 131

Query: 124 LRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVMR 183
           LRR+ S+EI S+ RLN+ L IR+DEI RL+ KL  +S   F +VEL+ MFSE++FN +MR
Sbjct: 132 LRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMR 191

Query: 184 VVTGKRYFGE--EVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAK 243
           +V+GKRY+G   +VS+  EAR FR ++ E    GGA+N  DF+  L+W  F   EK L K
Sbjct: 192 MVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKK 251

Query: 244 LTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRA 303
           ++KR D F+Q L+++ R          +T++D LL  Q S+PEYY+D+IIKG+++V+L A
Sbjct: 252 ISKRTDAFLQGLIDEHR----FGKRNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLA 311

Query: 304 GADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFR 363
           G D++SVT+EWAM+ LLN+PE++ KAK E+DT IG DR +DE D++ L YLQ+I+ ET R
Sbjct: 312 GTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETLR 371

Query: 364 LHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGN 423
           LH AAP+L+ H SS D ++ GY++P+ T+L+VNAW IHRDP LW DP  F+PERF  EG 
Sbjct: 372 LHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFEKEGE 431

Query: 424 ELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQK 483
               NKL+ FG+GRRACPGE ++ R  GL+LGLLIQC+EWK +G EK+DM E  GIT  K
Sbjct: 432 V---NKLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVEAKGITAGK 488

Query: 484 VQPLETMCR 486
              L  MC+
Sbjct: 492 KTSLNAMCK 488

BLAST of Carg25494 vs. ExPASy Swiss-Prot
Match: Q9FG65 (Cytochrome P450 81D1 OS=Arabidopsis thaliana OX=3702 GN=CYP81D1 PE=2 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.1e-143
Identity = 258/502 (51.39%), Postives = 360/502 (71.71%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRRRNLPPSPPSF-PIIGHLHLLQRPIHRNFHNIAAKY---- 63
           +F L+ L++ F FL+    +++NLPPSPP + PIIGHL LL+ PIHR   + +       
Sbjct: 13  IFSLIFLIISFKFLK---PKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHND 72

Query: 64  -GPIFTLRFGSRLALIVSSLQL-AEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFG 123
            G + +LR GSRL  +VSS ++ AEECF KNDVV ANRP+++IGK++GYN T +   P+G
Sbjct: 73  GGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYG 132

Query: 124 DHWRNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAK---VELKSMFSELSF 183
           DHWRNLRRL ++EIFST RLN  L +R DE+RRL+ +L   +  K   VELK M  +L+F
Sbjct: 133 DHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTF 192

Query: 184 NVVMRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKS 243
           N +MR++TGKRY+GEE ++E EA+  R+L+ +   +  + N VD++PIL+   F  YE  
Sbjct: 193 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSSYENR 252

Query: 244 LAKLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVL 303
           + KL +  DKF+Q L++D+R Q     E  +T++D LL LQ S+ EYY+D+IIKG+IL++
Sbjct: 253 VKKLGEETDKFLQGLIDDKRGQQ----ETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 312

Query: 304 LRAGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISE 363
           + AG ++++VT+EWA++ LLN+P+VISKA+ EID ++G DRLI+EADL+ L YL+ I+ E
Sbjct: 313 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 372

Query: 364 TFRLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLG 423
           T RLHPA P+L+ H +S DC +  YD+PRGT LLVNAWAIHRDP  WDDP SF+PERF  
Sbjct: 373 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 432

Query: 424 EGNELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGIT 483
           E       KL+ FG+GRRACPG  +A R++GL+LG LIQC+EW+ VG  +VDM EG G T
Sbjct: 433 EEEA---QKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 492

Query: 484 IQKVQPLETMCRALPIMDKVLS 496
           + K  PL+ +C+A P + K++S
Sbjct: 493 VPKAIPLKAICKARPFLHKIIS 502

BLAST of Carg25494 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 5.2e-143
Identity = 246/498 (49.40%), Postives = 353/498 (70.88%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGPIFT 63
           L  L  ++ + + L+    R +NLPP PP+ PIIG+LH L+ P+HR F  ++  YG IF+
Sbjct: 7   LLSLSFIITIKILLKITSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFS 66

Query: 64  LRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWRNLR 123
           L FGSRL ++VSS  LA ECFTKND++ ANRPR L GKY+ YN+TT+ +  +GDHWRNLR
Sbjct: 67  LWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLR 126

Query: 124 RLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSE------SFAKVELKSMFSELSFNVVM 183
           R+T++++ S  RLN+ L +R+DE  RL+ KL  +       F KVEL+   +E++FN +M
Sbjct: 127 RITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMM 186

Query: 184 RVVTGKRYFGE--EVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLA 243
           R+++GKRY+G+  +VS+  EA++FRE++ E     GA+N  DF+P+L+ +     EK   
Sbjct: 187 RMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCK 246

Query: 244 KLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLR 303
           ++ KR++ F++ L+E+ R   G       T++D LL+L  S+PEYYSD +IKG+I  +L 
Sbjct: 247 RIAKRSNAFLEGLIEEHRR--GNIHSDGGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLL 306

Query: 304 AGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETF 363
           AG D+++VT+EW M++LLN+PEV+ KAK E+DT+IG+++L+DE DL+ L YLQ IISET 
Sbjct: 307 AGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETL 366

Query: 364 RLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEG 423
           RLHP AP+LL HYSS DCT+  ++VP+ T++L N W IHRDPK W+D +SF+PERF  E 
Sbjct: 367 RLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEE 426

Query: 424 NELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQ 483
                NK++ FG+GRRACPG ++A R +G ++GLLIQC+EW+    EK+DM EG GIT+ 
Sbjct: 427 EV---NKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMP 486

Query: 484 KVQPLETMCRALPIMDKV 494
              PL  MC+ALPI + V
Sbjct: 487 MKIPLRAMCKALPIANDV 499

BLAST of Carg25494 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 508.8 bits (1309), Expect = 6.8e-143
Identity = 240/495 (48.48%), Postives = 361/495 (72.93%), Query Frame = 0

Query: 5   FVLLLLLLVFLFLQQFITRR-RNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGPIFT 64
           + +  L L F+F      R+ +NLPP PPS PIIG+LH L+RP+HR F  ++ KYG +F+
Sbjct: 9   YSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLSEKYGHVFS 68

Query: 65  LRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWRNLR 124
           L FGSRL ++VSS    ++CFTKNDVV ANRPR L GKY+ YN+TT+ +T +G+HWRNLR
Sbjct: 69  LWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRNLR 128

Query: 125 RLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVMRVV 184
           R+T++++ S  R+N+   IR+DE +RL+ +L  +S   FA++EL S   +++FN +MR++
Sbjct: 129 RITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMTFNNIMRMI 188

Query: 185 TGKRYFGE--EVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLT 244
           +GKRY+GE  + S+  EA +FR+++ E  +  GA+N  DFMP+L+++ F   EK L  ++
Sbjct: 189 SGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENLEKRLKDIS 248

Query: 245 KRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGA 304
            + D F++ L+E+ R +     E+ +T++D LL LQ S+PEYY+D+IIKG+ L +L AG 
Sbjct: 249 GKTDAFLRGLIEEHRTKK----ERANTMIDHLLNLQDSQPEYYTDQIIKGLALAMLLAGT 308

Query: 305 DSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLH 364
           DS++VT+EW+M+ LLN+PEV+ K K E+DT +G+DRL+DE+DL  L YL+ +I+ET RL+
Sbjct: 309 DSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVINETLRLY 368

Query: 365 PAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNEL 424
             AP+LL H +S++C + GY VP+ T++L+NAWAIHRDP+LW +  +F+PERF  +G   
Sbjct: 369 TPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERFEKKGE-- 428

Query: 425 LWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQ 484
              KLI FGMGRRACPGE +A+R I ++L LLIQC++WK +  +K+D+ E  G T+ K+ 
Sbjct: 429 -LEKLIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKIDLAERDGFTLTKLV 488

Query: 485 PLETMCRALPIMDKV 494
           PL+ MC++ P+++KV
Sbjct: 489 PLKAMCKSRPVINKV 496

BLAST of Carg25494 vs. ExPASy TrEMBL
Match: A0A6J1FUQ9 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111448607 PE=3 SV=1)

HSP 1 Score: 996.5 bits (2575), Expect = 4.0e-287
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP
Sbjct: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
           TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS
Sbjct: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW
Sbjct: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL
Sbjct: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMCRALPIMDKVLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Carg25494 vs. ExPASy TrEMBL
Match: A0A6J1J5B5 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111483621 PE=3 SV=1)

HSP 1 Score: 950.7 bits (2456), Expect = 2.5e-273
Identity = 473/499 (94.79%), Postives = 487/499 (97.60%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL V LLLLLVFLFLQQFITRRRNLPPSPPS PIIGHLHLLQRPIH+NFHNIAAKYGP
Sbjct: 1   MDFLSVFLLLLLVFLFLQQFITRRRNLPPSPPSVPIIGHLHLLQRPIHQNFHNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTP+GD WR
Sbjct: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPYGDLWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRLTSME+FSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV
Sbjct: 121 NLRRLTSMEVFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
           TGKRYFGEEVSNETEAREFRELMDE SRHGGASNW++FMPILKWIGFGEYEKSLAKLTKR
Sbjct: 181 TGKRYFGEEVSNETEAREFRELMDEISRHGGASNWINFMPILKWIGFGEYEKSLAKLTKR 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           ADKFMQKLVE RRNQTG +IEKQSTLLDRLLELQASEPEYYSDEIIKGV+LVLLRAG DS
Sbjct: 241 ADKFMQKLVEYRRNQTGFEIEKQSTLLDRLLELQASEPEYYSDEIIKGVVLVLLRAGVDS 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRL+DE+DLANLHYLQAII ETFRLHPA
Sbjct: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLVDESDLANLHYLQAIIYETFRLHPA 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DCTVAGYD+PRGTML+VNAWAIHRDPKLWDDPMSFRPERFLG GNELL 
Sbjct: 361 APMLLTHYSSDDCTVAGYDIPRGTMLMVNAWAIHRDPKLWDDPMSFRPERFLGAGNELLS 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKL+PFGMGRRACPGETMA RLIGLSLGLLIQCYEWKNVGY+KVDMTEGGGITIQKVQPL
Sbjct: 421 NKLVPFGMGRRACPGETMAQRLIGLSLGLLIQCYEWKNVGYQKVDMTEGGGITIQKVQPL 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMCRALPIMDKVLSNGLD
Sbjct: 481 ETMCRALPIMDKVLSNGLD 499

BLAST of Carg25494 vs. ExPASy TrEMBL
Match: A0A5A7SMV7 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold465G00110 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.4e-223
Identity = 382/499 (76.55%), Postives = 439/499 (87.98%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL    LL L FL LQQ  TRRRNLPPSPPSFPIIGHLHLLQRP HRNF NIAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IF+LRFGSRLA+IVSSLQ+A+ECFTK+D++FANRPRLL GKY GY++TT++T  +GDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRL +MEIFS  RLNA+L+ RKDEIRRLLVKLHSESFAKVEL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
            GKRY+GE+VS+E EAREFRELMDE SR GG S WVDFMPILKWIGFG YEK L K    
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           AD+F+Q+L+E+ RN+  L  E+QS+LL RLLELQ S+PEYY+D+IIKG++LVLLRAG D+
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           +SVT++WAMT+LLN+PEV++KAKAEIDTKIG+DR ++E+D+ANL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DC VAGY++PRGTMLLVNA AIHRDPKLWDDP SFRPERFLG GNEL  
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFG+GRRACPGE M LR++GL+LGLLIQCYEWK VGYE VD TE GGITI KV+P+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMC+  P+M KVLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Carg25494 vs. ExPASy TrEMBL
Match: A0A1S3C1R2 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103495911 PE=3 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.4e-223
Identity = 382/499 (76.55%), Postives = 439/499 (87.98%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL    LL L FL LQQ  TRRRNLPPSPPSFPIIGHLHLLQRP HRNF NIAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSPPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IF+LRFGSRLA+IVSSLQ+A+ECFTK+D++FANRPRLL GKY GY++TT++T  +GDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRL +MEIFS  RLNA+L+ RKDEIRRLLVKLHSESFAKVEL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
            GKRY+GE+VS+E EAREFRELMDE SR GG S WVDFMPILKWIGFG YEK L K    
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           AD+F+Q+L+E+ RN+  L  E+QS+LL RLLELQ S+PEYY+D+IIKG++LVLLRAG D+
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           +SVT++WAMT+LLN+PEV++KAKAEIDTKIG+DR ++E+D+ANL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DC VAGY++PRGTMLLVNA AIHRDPKLWDDP SFRPERFLG GNEL  
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFG+GRRACPGE M LR++GL+LGLLIQCYEWK VGYE VD TE GGITI KV+P+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMC+  P+M KVLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Carg25494 vs. ExPASy TrEMBL
Match: A0A5D3BGZ4 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold232G00980 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 2.0e-222
Identity = 381/499 (76.35%), Postives = 438/499 (87.78%), Query Frame = 0

Query: 1   MDFLFVLLLLLLVFLFLQQFITRRRNLPPSPPSFPIIGHLHLLQRPIHRNFHNIAAKYGP 60
           MDFL    LL L FL LQQ  TRRRNLPPS PSFPIIGHLHLLQRP HRNF NIAAKYGP
Sbjct: 1   MDFLSAFFLLFLSFLLLQQLRTRRRNLPPSQPSFPIIGHLHLLQRPTHRNFQNIAAKYGP 60

Query: 61  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 120
           IF+LRFGSRLA+IVSSLQ+A+ECFTK+D++FANRPRLL GKY GY++TT++T  +GDHWR
Sbjct: 61  IFSLRFGSRLAVIVSSLQIAQECFTKHDLIFANRPRLLSGKYFGYSWTTMTTASYGDHWR 120

Query: 121 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAKVELKSMFSELSFNVVMRVV 180
           NLRRL +MEIFS  RLNA+L+ RKDEIRRLLVKLHSESFAKVEL  MFSELSFN+VMRVV
Sbjct: 121 NLRRLAAMEIFSPTRLNASLAFRKDEIRRLLVKLHSESFAKVELTPMFSELSFNIVMRVV 180

Query: 181 TGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLTKR 240
            GKRY+GE+VS+E EAREFRELMDE SR GG S WVDFMPILKWIGFG YEK L K    
Sbjct: 181 AGKRYYGEKVSDEAEAREFRELMDEVSRQGGTSQWVDFMPILKWIGFGAYEKILTKSAIW 240

Query: 241 ADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGADS 300
           AD+F+Q+L+E+ RN+  L  E+QS+LL RLLELQ S+PEYY+D+IIKG++LVLLRAG D+
Sbjct: 241 ADRFVQELIEEHRNKKVLGREEQSSLLHRLLELQLSQPEYYTDQIIKGLVLVLLRAGIDT 300

Query: 301 TSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLHPA 360
           +SVT++WAMT+LLN+PEV++KAKAEIDTKIG+DR ++E+D+ANL+YLQAIISETFRLHP 
Sbjct: 301 SSVTLDWAMTELLNHPEVLAKAKAEIDTKIGQDRPVEESDIANLNYLQAIISETFRLHPP 360

Query: 361 APMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNELLW 420
           APMLLTHYSS+DC VAGY++PRGTMLLVNA AIHRDPKLWDDP SFRPERFLG GNEL  
Sbjct: 361 APMLLTHYSSDDCCVAGYNIPRGTMLLVNASAIHRDPKLWDDPTSFRPERFLGAGNELQT 420

Query: 421 NKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQPL 480
           NKLIPFG+GRRACPGE M LR++GL+LGLLIQCYEWK VGYE VD TE GGITI KV+P+
Sbjct: 421 NKLIPFGIGRRACPGEIMGLRVVGLTLGLLIQCYEWKKVGYENVDTTEYGGITILKVKPV 480

Query: 481 ETMCRALPIMDKVLSNGLD 500
           ETMC+  P+M KVLSNGLD
Sbjct: 481 ETMCKPCPVMVKVLSNGLD 499

BLAST of Carg25494 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 536.6 bits (1381), Expect = 2.2e-152
Identity = 266/501 (53.09%), Postives = 371/501 (74.05%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRRRNLPPSPP-SFPIIGHLHLLQRPIHRNFHNIAAKY--GP 63
           +F +L ++L  ++L   + R+ NLPPSP  S P+IGHL LL+ PIHR F +++      P
Sbjct: 7   IFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNAP 66

Query: 64  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 123
           IF+LR G+RL  + SS  +AEECFTKNDVV ANRP  ++ K++ Y++TT+    +GDHWR
Sbjct: 67  IFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHWR 126

Query: 124 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKL---HSESFAKVELKSMFSELSFNVVM 183
           NLRR+ S+EIFS  RLN+ LSIRKDEIRRL+ +L    S+ F KV++KSM S+L+FN ++
Sbjct: 127 NLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNIL 186

Query: 184 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 243
           R+V GKRY+G+ V ++ EA+  R+L+ +     GA N VD++P+L+ +   +YE  + KL
Sbjct: 187 RMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLV--SDYETRVKKL 246

Query: 244 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 303
             R D+F+Q LV+++R       EK +T++D LL LQ S+P+Y++D IIKG +L L+ AG
Sbjct: 247 AGRLDEFLQGLVDEKREAK----EKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAG 306

Query: 304 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 363
            D+++VT+EWA++ +LN+P+V++KA+ EID KIG DRL+DE+D++NL YLQ I+SET RL
Sbjct: 307 TDTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRL 366

Query: 364 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 423
           +PAAPMLL H +S DC VAGYD+PRGT+LL N WAIHRDP+LWDDPMSF+PERF  EG  
Sbjct: 367 YPAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEA 426

Query: 424 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 483
               KL+PFG+GRRACPG  +A RLI L+LG LIQC EW+ +G E+VDM+EG G+T+ K 
Sbjct: 427 ---QKLMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIG-EEVDMSEGKGVTMPKA 486

Query: 484 QPLETMCRALPIMDKVLSNGL 499
           +PLE MCRA P + K+ +  +
Sbjct: 487 KPLEAMCRARPSVVKIFNESV 497

BLAST of Carg25494 vs. TAIR 10
Match: AT4G37320.1 (cytochrome P450, family 81, subfamily D, polypeptide 5 )

HSP 1 Score: 526.9 bits (1356), Expect = 1.7e-149
Identity = 263/499 (52.71%), Postives = 352/499 (70.54%), Query Frame = 0

Query: 9   LLLLVFLFLQQFI-------TRRRNLPPSPP-SFPIIGHLHLLQRPIHRNFHNIAAKYG- 68
           +LLL FLFL   I        R+ NLPPSP    P+IGHLHLL++P+HR FH+I+   G 
Sbjct: 6   ILLLSFLFLTISIKLLLTKSNRKPNLPPSPAYPLPVIGHLHLLKQPVHRTFHSISKSLGN 65

Query: 69  -PIFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDH 128
            PIF LR G+RL  ++SS  +AEECFTKNDVV ANRP +++ K++GYNFT +    +GDH
Sbjct: 66  APIFHLRLGNRLVYVISSHSIAEECFTKNDVVLANRPDIIMAKHVGYNFTNMIAASYGDH 125

Query: 129 WRNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNV 188
           WRNLRR+ ++EIFS+ R++   SIRKDEIRRL+  L  +S   F +VELKS+ + L+FN 
Sbjct: 126 WRNLRRIAAVEIFSSHRISTFSSIRKDEIRRLITHLSRDSLHGFVEVELKSLLTNLAFNN 185

Query: 189 VMRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLA 248
           ++ +V GKRY+G    +  EA+  REL+ E     G+ N  D++P + W+    +E    
Sbjct: 186 IIMMVAGKRYYGTGTEDNDEAKLVRELIAEIMAGAGSGNLADYLPSINWV--TNFENQTK 245

Query: 249 KLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLR 308
            L  R D+ +QKLV+++R +     EK  TL+D LL  Q +EPEYY+D IIKG+IL L+ 
Sbjct: 246 ILGNRLDRVLQKLVDEKRAEK----EKGQTLIDHLLSFQETEPEYYTDVIIKGIILALVL 305

Query: 309 AGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETF 368
           AG D++SVT+EWAM+ LLN+PE++ KA+AEID KIG DRL++E+D+ NLHYLQ I+SET 
Sbjct: 306 AGTDTSSVTLEWAMSNLLNHPEILEKARAEIDDKIGSDRLVEESDIVNLHYLQNIVSETL 365

Query: 369 RLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEG 428
           RL+PA P+LL H+SS++C VAGYD+PR T+LL N WA+HRDP LW++P  F+PERF  EG
Sbjct: 366 RLYPAVPLLLPHFSSDECKVAGYDMPRRTLLLTNVWAMHRDPGLWEEPERFKPERFEKEG 425

Query: 429 NELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQ 488
                 KL+PFGMGRRACPG  +  RL+ L+LG LIQ +EW+ VG E VDMTEG GIT+ 
Sbjct: 426 EA---RKLMPFGMGRRACPGAELGKRLVSLALGCLIQSFEWERVGAELVDMTEGEGITMP 485

Query: 489 KVQPLETMCRALPIMDKVL 495
           K  PL  MC+A  I+ K +
Sbjct: 486 KATPLRAMCKARAIVGKTI 495

BLAST of Carg25494 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 515.4 bits (1326), Expect = 5.2e-146
Identity = 260/497 (52.31%), Postives = 354/497 (71.23%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRRRNLPPSPP-SFPIIGHLHLLQRPIHRNFHNIAAKYG--P 63
           +F    ++L  +FL   I R+ NLPPSP  + P+IGHL LL+ P+HR F +++   G  P
Sbjct: 7   IFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVSQSLGDAP 66

Query: 64  IFTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWR 123
           I +LR G+RL  +VSS  +AEECFTKNDV+ ANR   +  K++ Y  +T+ +  + +HWR
Sbjct: 67  IISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSASYSEHWR 126

Query: 124 NLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKL---HSESFAKVELKSMFSELSFNVVM 183
           NLRR+ ++EIFS  RLN+  SIR+DEIRRL+ +L    S  F KVE+KSMFS+L+FN ++
Sbjct: 127 NLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSDLTFNNII 186

Query: 184 RVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKL 243
           R++ GK Y+G+   ++ EA+  R L+ E     G  N  D++PIL WI +   E  + KL
Sbjct: 187 RMLAGKCYYGDGKEDDPEAKRVRTLIAEAMSSSGPGNAADYIPILTWITYS--ETRIKKL 246

Query: 244 TKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAG 303
             R D+F+Q LV+++R     + +K++T++D LL LQ ++PEYY D IIKG +L L+  G
Sbjct: 247 AGRLDEFLQGLVDEKREG---KEKKENTMVDHLLCLQETQPEYYMDRIIKGTMLSLIAGG 306

Query: 304 ADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRL 363
            D+T+VT+EWA++ LLNNPEV++KA+ EID  IG DRL++E+D+ NL YLQ I+SET RL
Sbjct: 307 TDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIVSETLRL 366

Query: 364 HPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNE 423
           +PAAPMLL H +S DC V GYD+PRGTMLL NAWAIHRDP LWDDP SF+PERF  EG  
Sbjct: 367 YPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERFEKEGEA 426

Query: 424 LLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKV 483
               KL+PFG+GRRACPG  +A RL+ LSLG LIQC+EW+ +G E+VDMTEG G+T+ K 
Sbjct: 427 ---KKLMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEVDMTEGPGLTMPKA 486

Query: 484 QPLETMCRALPIMDKVL 495
           +PLE MCRA   + K+L
Sbjct: 487 RPLEAMCRARDFVGKIL 495

BLAST of Carg25494 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 513.8 bits (1322), Expect = 1.5e-145
Identity = 257/496 (51.81%), Postives = 350/496 (70.56%), Query Frame = 0

Query: 5   FVLLLLLLVFLFLQQFITRRRNLPPSPP-SFPIIGHLHLLQRPIHRNFHNIAAKYG--PI 64
           F+ L  L +FL  +    R+ NLPPSP  S P+IGHLHLL+ P+HR F +++   G  P+
Sbjct: 11  FLSLFTLAIFLLTRS--KRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLSKSIGNAPV 70

Query: 65  FTLRFGSRLALIVSSLQLAEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFGDHWRN 124
           F LR G+RL  ++SS  +AEECFTKNDVV ANRP+  I K+LGYN T + +  +GDHWRN
Sbjct: 71  FHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSASYGDHWRN 130

Query: 125 LRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSES---FAKVELKSMFSELSFNVVMR 184
           LRR+ ++EIFST RLN+ L IRKDEIRRL+  L  +S   F +VE+K++ + L+ N  +R
Sbjct: 131 LRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTNLASNTTIR 190

Query: 185 VVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKSLAKLT 244
           ++ GKRYFGE+     +A+  + L+ E     GA N +D++ IL+W+    YEK +  L 
Sbjct: 191 MLAGKRYFGED---NDDAKLVKNLVSEAVTSAGAGNPIDYLSILRWV--SSYEKRIKNLG 250

Query: 245 KRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVLLRAGA 304
            R D F+QKLV+++R +     EK  T++D LL LQ  +P+YY+D IIKG+IL L+ AG 
Sbjct: 251 NRFDTFLQKLVDEKRAEK----EKGETMIDHLLALQDIQPDYYTDVIIKGIILTLIIAGT 310

Query: 305 DSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISETFRLH 364
           D++SVT+EWAM+ LLN+PE++ KA+ EID K+G DRL+DE+D+ NL YLQ+I+ ET R++
Sbjct: 311 DTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLETLRMY 370

Query: 365 PAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLGEGNEL 424
           PA P+LL H SS DC V GYD+P GTM+L NAWA+HRDP++W+DP  F+PERF  EG   
Sbjct: 371 PAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEKEGEA- 430

Query: 425 LWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGITIQKVQ 484
              KLI FGMGRRACPG  +A RLI  +LG L+QC+EW+ VG + VDMTE  G T+ K  
Sbjct: 431 --EKLISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDMTEDKGATLPKAI 490

Query: 485 PLETMCRALPIMDKVL 495
           PL  MC+A  I+DK++
Sbjct: 491 PLRAMCKARSIVDKLI 492

BLAST of Carg25494 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 510.0 bits (1312), Expect = 2.2e-144
Identity = 258/502 (51.39%), Postives = 360/502 (71.71%), Query Frame = 0

Query: 4   LFVLLLLLLVFLFLQQFITRRRNLPPSPPSF-PIIGHLHLLQRPIHRNFHNIAAKY---- 63
           +F L+ L++ F FL+    +++NLPPSPP + PIIGHL LL+ PIHR   + +       
Sbjct: 13  IFSLIFLIISFKFLK---PKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSETLDHND 72

Query: 64  -GPIFTLRFGSRLALIVSSLQL-AEECFTKNDVVFANRPRLLIGKYLGYNFTTISTTPFG 123
            G + +LR GSRL  +VSS ++ AEECF KNDVV ANRP+++IGK++GYN T +   P+G
Sbjct: 73  GGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMIAAPYG 132

Query: 124 DHWRNLRRLTSMEIFSTARLNAALSIRKDEIRRLLVKLHSESFAK---VELKSMFSELSF 183
           DHWRNLRRL ++EIFST RLN  L +R DE+RRL+ +L   +  K   VELK M  +L+F
Sbjct: 133 DHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPMLMDLTF 192

Query: 184 NVVMRVVTGKRYFGEEVSNETEAREFRELMDETSRHGGASNWVDFMPILKWIGFGEYEKS 243
           N +MR++TGKRY+GEE ++E EA+  R+L+ +   +  + N VD++PIL+   F  YE  
Sbjct: 193 NNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRL--FSSYENR 252

Query: 244 LAKLTKRADKFMQKLVEDRRNQTGLQIEKQSTLLDRLLELQASEPEYYSDEIIKGVILVL 303
           + KL +  DKF+Q L++D+R Q     E  +T++D LL LQ S+ EYY+D+IIKG+IL++
Sbjct: 253 VKKLGEETDKFLQGLIDDKRGQQ----ETGTTMIDHLLVLQKSDIEYYTDQIIKGIILIM 312

Query: 304 LRAGADSTSVTMEWAMTQLLNNPEVISKAKAEIDTKIGRDRLIDEADLANLHYLQAIISE 363
           + AG ++++VT+EWA++ LLN+P+VISKA+ EID ++G DRLI+EADL+ L YL+ I+ E
Sbjct: 313 VIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNIVLE 372

Query: 364 TFRLHPAAPMLLTHYSSNDCTVAGYDVPRGTMLLVNAWAIHRDPKLWDDPMSFRPERFLG 423
           T RLHPA P+L+ H +S DC +  YD+PRGT LLVNAWAIHRDP  WDDP SF+PERF  
Sbjct: 373 TLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPERFEK 432

Query: 424 EGNELLWNKLIPFGMGRRACPGETMALRLIGLSLGLLIQCYEWKNVGYEKVDMTEGGGIT 483
           E       KL+ FG+GRRACPG  +A R++GL+LG LIQC+EW+ VG  +VDM EG G T
Sbjct: 433 EEEA---QKLLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVEVDMKEGVGNT 492

Query: 484 IQKVQPLETMCRALPIMDKVLS 496
           + K  PL+ +C+A P + K++S
Sbjct: 493 VPKAIPLKAICKARPFLHKIIS 502