Carg25479 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25479 vs. NCBI nr
Match: KAG7011015.1 (putative folate-biopterin transporter 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 837.4 bits (2162), Expect = 5.6e-239
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. NCBI nr
Match: KAG6571212.1 (putative folate-biopterin transporter 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 834.3 bits (2154), Expect = 4.8e-238
Identity = 439/440 (99.77%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. NCBI nr
Match: XP_022943764.1 (probable folate-biopterin transporter 7 [Cucurbita moschata] >XP_022943765.1 probable folate-biopterin transporter 7 [Cucurbita moschata])

HSP 1 Score: 833.2 bits (2151), Expect = 1.1e-237
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMG+PDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. NCBI nr
Match: XP_023513027.1 (probable folate-biopterin transporter 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 831.6 bits (2147), Expect = 3.1e-237
Identity = 437/440 (99.32%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTE+LKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEYLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMGVPDF+YVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. NCBI nr
Match: XP_022986535.1 (probable folate-biopterin transporter 7 [Cucurbita maxima] >XP_022986536.1 probable folate-biopterin transporter 7 [Cucurbita maxima])

HSP 1 Score: 829.3 bits (2141), Expect = 1.5e-236
Identity = 436/440 (99.09%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTE+LKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTENLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMG+PDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCT+LPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. ExPASy Swiss-Prot
Match: Q9XIQ7 (Probable folate-biopterin transporter 7 OS=Arabidopsis thaliana OX=3702 GN=At1g64890 PE=2 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 1.5e-149
Identity = 277/428 (64.72%), Postives = 343/428 (80.14%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 68  DAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVAND 127
           D+VY FGQHRIPYIA+GALLQA+SW+ IA +S S++S+  +SIYLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFG 187
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGGI I  FS Q  FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 188 LLLLLQFLLTIIVRERSLNLPQS-STSAGIKKQLSQLFVVLKRPEIVYSITWFAASYSII 247
           +L LLQFL+TI +RE+SLNLP++ S + GI+K LS L  VL++PEI YSI W A S +++
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 248 PALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVLM 307
           P LTGTMFFYQT+ LKI++S+LGISKV GQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 308 AVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGSL 367
           A F+ISD+LFVKGVYR++GV D VYV+ FSG  E L +FKILPF+VL+A+LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 368 MAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCSWCIPDD 427
           MAFVMSAIALAFIVSG LG+ LAS+VGVT ++FSG   GL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 428 VKAKGRDK 435
            + K + K
Sbjct: 437 AETKEKSK 439

BLAST of Carg25479 vs. ExPASy Swiss-Prot
Match: O22780 (Probable folate-biopterin transporter 9, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g33280 PE=3 SV=2)

HSP 1 Score: 329.7 bits (844), Expect = 5.0e-89
Identity = 181/410 (44.15%), Postives = 274/410 (66.83%), Query Frame = 0

Query: 16  LGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYIFGQ 75
           LGYWVQG RCF W+A+NF +   LN+ PSTLQL+Q +A+LPMVAKPLYG+LSD +YI G 
Sbjct: 90  LGYWVQGLRCFSWLALNFHMAHCLNLKPSTLQLVQYTASLPMVAKPLYGVLSDVLYIGGA 149

Query: 76  HRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVANDAIVAEAGK 135
            R+PYI++G LLQ ++W  +A    +   + ++  ++LLSNLGASI EV+ DA+VAE G 
Sbjct: 150 RRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSNLGASITEVSQDALVAEYG- 209

Query: 136 QPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFGLLLLLQFL 195
                   R   +G LQ++  +AS+ GGILGNLLGG  + +  P+ +FL F  LL LQ +
Sbjct: 210 -------LRYQING-LQSYALMASAVGGILGNLLGGYCLLKTPPRILFLAFTALLSLQLI 269

Query: 196 LTIIVRERSLNLPQ--------SSTSAGIKKQLSQLFVVLKRPEIVYSITWFAASYSIIP 255
           +++  +E S+NLP+        SS    +KKQ   L  +++  EI   + W  +S +++P
Sbjct: 270 VSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQVDEISQPLIWIVSSIALVP 329

Query: 256 ALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMA 315
            L+G++F YQT+ L ++ SV+G+SKVIGQ  LL   V+Y+R+ K +P R LI  +Q+L A
Sbjct: 330 LLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYWKKLPMRALIHIVQLLYA 389

Query: 316 VFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGSLM 375
             ++ D + VK +    G+ +  +V+ FS ++E+L  FKILPFSVL+A +CP GCEGS+ 
Sbjct: 390 FSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKILPFSVLLANMCPGGCEGSIT 449

Query: 376 AFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPL 418
           +F+ S + L+ +VSG  GV +A+ +G+T+  +S LP G++IQ++  ++PL
Sbjct: 450 SFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILIQSLAALVPL 490

BLAST of Carg25479 vs. ExPASy Swiss-Prot
Match: F4I5Q2 (Probable folate-biopterin transporter 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g04570 PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 6.5e-89
Identity = 187/419 (44.63%), Postives = 273/419 (65.16%), Query Frame = 0

Query: 10  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 69
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 70  VYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVANDAI 129
           +YI    R+PYIAIG  LQ ++W  +     +   + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 130 VAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFGLL 189
           VAE G         R   +G LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYG--------LRYRING-LQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 190 LLLQFLLTIIVRERSLNLPQ----SSTSAGIKKQLSQLFVVLKRPEIVYSITWFAASYSI 249
           L LQ ++++  +E S  LP+    SS    +KKQ+S L   ++  EI   + W   S ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 250 IPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVL 309
           +P L+G++F YQT+ L ++ SV+G+SKVIGQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 310 MAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGS 369
             + ++ D + VK +    G+ + VYV+ FS L+E+L  FKILPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 370 LMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCSWCIP 425
           + +F+ S + L+ IVS  LGV LA+ +G+T++ +S L +G++IQ++  + PL     +P
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVP 496

BLAST of Carg25479 vs. ExPASy Swiss-Prot
Match: Q8RWQ5 (Probable folate-biopterin transporter 4 OS=Arabidopsis thaliana OX=3702 GN=At5g54860 PE=2 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.1e-55
Identity = 154/467 (32.98%), Postives = 235/467 (50.32%), Query Frame = 0

Query: 18  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYIFGQHR 77
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 78  IPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVANDAIVAEAGKQP 137
            PY+ I  +L  V W+ +   S S  S   + I+L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 138 TSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFGLLLLLQFLLT 197
              +  +AS +G+LQ+  W A + GG+ G+LLGG  +     + +FL F +L  LQ L  
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 198 IIV-------------------RERSL----NLPQSSTS-----AGIKK----------- 257
            +V                    E+SL    N P +S S      G KK           
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 258 --------QLSQLFVVL-----------KRPEIVYSITWFAASYSIIPALTGTMFFYQTE 317
                     SQ F  L           K+P I+  + WF  ++  +P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 318 HLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMAVFMISDMLFVKG 377
            L+++++ LG ++V+G   L+    IYNR+L+ +  RK +    + ++V ++ DM+ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 378 VYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGSLMAFVMSAIALAFI 427
                GV D   V+  S L + +   K +PF +L  +LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of Carg25479 vs. ExPASy Swiss-Prot
Match: Q9SKZ5 (Folate-biopterin transporter 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g32040 PE=1 SV=2)

HSP 1 Score: 168.3 bits (425), Expect = 1.9e-40
Identity = 122/430 (28.37%), Postives = 213/430 (49.53%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 68  DAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVAND 127
           D+V +FG  R  Y+ +  LL A SW   + M+    S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFG 187
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 188 LLLLLQFLLTIIVRERSLNLPQSSTSAGI-----------KKQLSQLFVVLKRPEIVYSI 247
           LL L+   + ++V E+ +  P S     I           K+ + QL+  +K+P  V+  
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPN-VFLP 363

Query: 248 TWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSR 307
           T F   +   P     MF++ T  L      LG  K++   A LL   +YN  LK++P R
Sbjct: 364 TLFIFLWQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 308 KLISSIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQ 367
           K+     +      ++ ++ V G  R++G+ D  + +  S +  VL     +P  VL A+
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 368 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLP 427
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L   +I+  + ++LP
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543

BLAST of Carg25479 vs. ExPASy TrEMBL
Match: A0A6J1FV96 (probable folate-biopterin transporter 7 OS=Cucurbita moschata OX=3662 GN=LOC111448420 PE=3 SV=1)

HSP 1 Score: 833.2 bits (2151), Expect = 5.1e-238
Identity = 438/440 (99.55%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMG+PDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. ExPASy TrEMBL
Match: A0A6J1JEB7 (probable folate-biopterin transporter 7 OS=Cucurbita maxima OX=3661 GN=LOC111484245 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 7.4e-237
Identity = 436/440 (99.09%), Postives = 439/440 (99.77%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
           EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA
Sbjct: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
           ASYSIIPALTGTMFFYQTE+LKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 ASYSIIPALTGTMFFYQTENLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMG+PDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGMPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCT+LPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQQ 441
           WCIPDDVKAKGRDKDSKKQQ
Sbjct: 421 WCIPDDVKAKGRDKDSKKQQ 440

BLAST of Carg25479 vs. ExPASy TrEMBL
Match: A0A0A0LTK8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G431200 PE=3 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 9.1e-219
Identity = 403/439 (91.80%), Postives = 414/439 (94.31%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           MS+SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MSKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSS +AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSMNAGINKQLSELAAVLKRPEIAYSIIWLA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLIS
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIS 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQVLMAVFMISDMLFVKGVYREMGVPDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPL CS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLCCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Carg25479 vs. ExPASy TrEMBL
Match: A0A5A7VD92 (Putative folate-biopterin transporter 7 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001470 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 402/439 (91.57%), Postives = 415/439 (94.53%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M++SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSST+AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQV MAVFMISDMLFVKGVYREMGVPDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Carg25479 vs. ExPASy TrEMBL
Match: A0A1S3CHR5 (probable folate-biopterin transporter 7 OS=Cucumis melo OX=3656 GN=LOC103501083 PE=3 SV=1)

HSP 1 Score: 768.8 bits (1984), Expect = 1.2e-218
Identity = 402/439 (91.57%), Postives = 415/439 (94.53%), Query Frame = 0

Query: 1   MSESNANNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60
           M++SN++NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK
Sbjct: 1   MAKSNSDNPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAK 60

Query: 61  PLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGAS 120
           PLYGILSDAVYI GQHRIPYIAIGA LQAVSW+PIA M PS ISVFTISIYLLLSNLGAS
Sbjct: 61  PLYGILSDAVYISGQHRIPYIAIGAFLQAVSWLPIALMPPSRISVFTISIYLLLSNLGAS 120

Query: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQ 180
           IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGG+ IDRFSPQ
Sbjct: 121 IAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGVAIDRFSPQ 180

Query: 181 EMFLFFGLLLLLQFLLTIIVRERSLNLPQSSTSAGIKKQLSQLFVVLKRPEIVYSITWFA 240
            MFLFFGLLL LQF LTIIVRERSLNLPQSST+AGI KQLS+L  VLKRPEI YSI W A
Sbjct: 181 AMFLFFGLLLSLQFFLTIIVRERSLNLPQSSTNAGINKQLSELAAVLKRPEIAYSIMWLA 240

Query: 241 ASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLIS 300
            SYSI PALTGTMFFYQTEHLKIESSVLGISKV GQAALLLWGVIYNRHLKSIPSRKLI+
Sbjct: 241 VSYSITPALTGTMFFYQTEHLKIESSVLGISKVFGQAALLLWGVIYNRHLKSIPSRKLIA 300

Query: 301 SIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPP 360
           SIQV MAVFMISDMLFVKGVYREMGVPDF+YVVLFSGLSEVLLFFKILPFSVLIA LCPP
Sbjct: 301 SIQVSMAVFMISDMLFVKGVYREMGVPDFIYVVLFSGLSEVLLFFKILPFSVLIALLCPP 360

Query: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCS 420
           GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTAN+FSGL  GLIIQAVCT+LPLSCS
Sbjct: 361 GCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANDFSGLARGLIIQAVCTLLPLSCS 420

Query: 421 WCIPDDVKAKGRDKDSKKQ 440
           WCIPDD+KAK R KDSKKQ
Sbjct: 421 WCIPDDIKAKDRRKDSKKQ 439

BLAST of Carg25479 vs. TAIR 10
Match: AT1G64890.1 (Major facilitator superfamily protein )

HSP 1 Score: 530.8 bits (1366), Expect = 1.0e-150
Identity = 277/428 (64.72%), Postives = 343/428 (80.14%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           NPIR +LG G++VQGFR FPW+  NFFL + L V+PS LQLLQNSANLPMVAKP+YG++S
Sbjct: 17  NPIRWLLGFGFFVQGFRGFPWLGANFFLTEQLRVNPSVLQLLQNSANLPMVAKPIYGVVS 76

Query: 68  DAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVAND 127
           D+VY FGQHRIPYIA+GALLQA+SW+ IA +S S++S+  +SIYLLLSNLGAS+ EVAND
Sbjct: 77  DSVYFFGQHRIPYIAVGALLQAISWLAIAFLSRSNVSILALSIYLLLSNLGASLVEVAND 136

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFG 187
           AIVAE+GKQ TS      + SGEL +FVW+ SS GGILGNLLGGI I  FS Q  FL FG
Sbjct: 137 AIVAESGKQKTSE-----TQSGELPSFVWMVSSLGGILGNLLGGIAIKTFSAQSTFLVFG 196

Query: 188 LLLLLQFLLTIIVRERSLNLPQS-STSAGIKKQLSQLFVVLKRPEIVYSITWFAASYSII 247
           +L LLQFL+TI +RE+SLNLP++ S + GI+K LS L  VL++PEI YSI W A S +++
Sbjct: 197 ILALLQFLVTINIREKSLNLPENPSPAGGIRKHLSDLSHVLRKPEISYSIAWIAVSTAVV 256

Query: 248 PALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVLM 307
           P LTGTMFFYQT+ LKI++S+LGISKV GQ A+LLWG  YNR LK++  RKL+++IQV +
Sbjct: 257 PVLTGTMFFYQTKFLKIDASLLGISKVFGQIAMLLWGFAYNRWLKAMRPRKLLTAIQVTI 316

Query: 308 AVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGSL 367
           A F+ISD+LFVKGVYR++GV D VYV+ FSG  E L +FKILPF+VL+A+LCPPGCEGSL
Sbjct: 317 AFFVISDLLFVKGVYRDLGVSDSVYVLFFSGFLETLFYFKILPFTVLMARLCPPGCEGSL 376

Query: 368 MAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCSWCIPDD 427
           MAFVMSAIALAFIVSG LG+ LAS+VGVT ++FSG   GL I+A C  +PL  +  I D+
Sbjct: 377 MAFVMSAIALAFIVSGYLGIVLASFVGVTEDDFSGFTRGLAIEACCVGIPLILTSWIYDE 436

Query: 428 VKAKGRDK 435
            + K + K
Sbjct: 437 AETKEKSK 439

BLAST of Carg25479 vs. TAIR 10
Match: AT1G04570.1 (Major facilitator superfamily protein )

HSP 1 Score: 329.3 bits (843), Expect = 4.6e-90
Identity = 187/419 (44.63%), Postives = 273/419 (65.16%), Query Frame = 0

Query: 10  IRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDA 69
           I  + GLGYWVQG RCFPW+A+NF +   L + PSTLQL+Q S +LPMVAKPLYG+LSD 
Sbjct: 87  ISVLCGLGYWVQGSRCFPWLALNFHMVHSLALQPSTLQLVQYSCSLPMVAKPLYGVLSDV 146

Query: 70  VYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVANDAI 129
           +YI    R+PYIAIG  LQ ++W  +     +   + ++   +LLSNLGASI EVA DA+
Sbjct: 147 LYIGSGRRVPYIAIGVFLQVLAWGSMGIFQGAREVLPSLVACVLLSNLGASITEVAKDAL 206

Query: 130 VAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFGLL 189
           VAE G         R   +G LQ++  +AS+AGG+LGNLLGG  +    P+  FL F  L
Sbjct: 207 VAEYG--------LRYRING-LQSYALMASAAGGVLGNLLGGYLLLTTPPKISFLVFSAL 266

Query: 190 LLLQFLLTIIVRERSLNLPQ----SSTSAGIKKQLSQLFVVLKRPEIVYSITWFAASYSI 249
           L LQ ++++  +E S  LP+    SS    +KKQ+S L   ++  EI   + W   S ++
Sbjct: 267 LSLQLVVSLSSKEESFGLPRIAETSSVLESVKKQISNLKEAIQADEISQPLIWAVVSIAM 326

Query: 250 IPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVL 309
           +P L+G++F YQT+ L ++ SV+G+SKVIGQ  LL   V+Y+R+LK++P R LI  IQ+L
Sbjct: 327 VPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDRYLKTLPMRPLIHIIQLL 386

Query: 310 MAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGS 369
             + ++ D + VK +    G+ + VYV+ FS L+E+L  FKILPF+V +A +CP GCEGS
Sbjct: 387 YGLSILLDYILVKQINLGFGISNEVYVLCFSSLAEILAQFKILPFAVRLASMCPQGCEGS 446

Query: 370 LMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLPLSCSWCIP 425
           + +F+ S + L+ IVS  LGV LA+ +G+T++ +S L +G++IQ++  + PL     +P
Sbjct: 447 VTSFLASTLCLSQIVSAFLGVGLANLIGITSSNYSNLSSGILIQSLAALAPLCFMHLVP 496

BLAST of Carg25479 vs. TAIR 10
Match: AT2G33280.1 (Major facilitator superfamily protein )

HSP 1 Score: 271.2 bits (692), Expect = 1.5e-72
Identity = 155/369 (42.01%), Postives = 241/369 (65.31%), Query Frame = 0

Query: 57  MVAKPLYGILSDAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSN 116
           MVAKPLYG+LSD +YI G  R+PYI++G LLQ ++W  +A    +   + ++  ++LLSN
Sbjct: 1   MVAKPLYGVLSDVLYIGGARRVPYISVGVLLQGLAWGSLAIFPGAREVLPSLMAFILLSN 60

Query: 117 LGASIAEVANDAIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDR 176
           LGASI EV+ DA+VAE G         R   +G LQ++  +AS+ GGILGNLLGG  + +
Sbjct: 61  LGASITEVSQDALVAEYG--------LRYQING-LQSYALMASAVGGILGNLLGGYCLLK 120

Query: 177 FSPQEMFLFFGLLLLLQFLLTIIVRERSLNLPQ--------SSTSAGIKKQLSQLFVVLK 236
             P+ +FL F  LL LQ ++++  +E S+NLP+        SS    +KKQ   L  +++
Sbjct: 121 TPPRILFLAFTALLSLQLIVSLSSKEESVNLPRIGEVTPEISSVLGIVKKQFLDLKGIVQ 180

Query: 237 RPEIVYSITWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNR 296
             EI   + W  +S +++P L+G++F YQT+ L ++ SV+G+SKVIGQ  LL   V+Y+R
Sbjct: 181 VDEISQPLIWIVSSIALVPLLSGSVFCYQTQVLNLDPSVIGMSKVIGQLMLLCLTVVYDR 240

Query: 297 HLKSIPSRKLISSIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKIL 356
           + K +P R LI  +Q+L A  ++ D + VK +    G+ +  +V+ FS ++E+L  FKIL
Sbjct: 241 YWKKLPMRALIHIVQLLYAFSLLFDYILVKQINLAFGISNTAFVLCFSSVAEILAQFKIL 300

Query: 357 PFSVLIAQLCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLII 416
           PFSVL+A +CP GCEGS+ +F+ S + L+ +VSG  GV +A+ +G+T+  +S LP G++I
Sbjct: 301 PFSVLLANMCPGGCEGSITSFLASTLCLSSVVSGFTGVGMANMIGITSKNYSNLPAGILI 360

Query: 417 QAVCTVLPL 418
           Q++  ++PL
Sbjct: 361 QSLAALVPL 360

BLAST of Carg25479 vs. TAIR 10
Match: AT5G54860.1 (Major facilitator superfamily protein )

HSP 1 Score: 218.0 bits (554), Expect = 1.5e-56
Identity = 154/467 (32.98%), Postives = 235/467 (50.32%), Query Frame = 0

Query: 18  YWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILSDAVYIFGQHR 77
           Y+ QGFR F W AV++ LKD L + PS  Q + + A  P   KPLYGI+SD + I G+ R
Sbjct: 24  YFTQGFRSFVWTAVSYQLKDRLQLSPSASQFVFSVAFFPWSIKPLYGIISDCIPIGGKKR 83

Query: 78  IPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVANDAIVAEAGKQP 137
            PY+ I  +L  V W+ +   S S  S   + I+L + NLG+++A+V  DA++AEA    
Sbjct: 84  TPYLVISTVLSLVPWLVLGLDSTSRSSSLYLMIFLTVQNLGSAMADVVIDAMIAEA---- 143

Query: 138 TSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFGLLLLLQFLLT 197
              +  +AS +G+LQ+  W A + GG+ G+LLGG  +     + +FL F +L  LQ L  
Sbjct: 144 --VRLEKASFAGDLQSVSWFAMAVGGVCGSLLGGYALSNLKIETIFLLFTVLPALQLLSC 203

Query: 198 IIV-------------------RERSL----NLPQSSTS-----AGIKK----------- 257
            +V                    E+SL    N P +S S      G KK           
Sbjct: 204 ALVEESPANNEPLPELLDSNEFEEKSLTSNDNYPDTSKSNTRRRKGQKKGKKGDSNGKSE 263

Query: 258 --------QLSQLFVVL-----------KRPEIVYSITWFAASYSIIPALTGTMFFYQTE 317
                     SQ F  L           K+P I+  + WF  ++  +P L+  MF+YQTE
Sbjct: 264 TQKKQSKSLASQWFQSLKAATFGLGRAFKQPIILRPMAWFFIAHITVPNLSTVMFYYQTE 323

Query: 318 HLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSRKLISSIQVLMAVFMISDMLFVKG 377
            L+++++ LG ++V+G   L+    IYNR+L+ +  RK +    + ++V ++ DM+ V  
Sbjct: 324 VLQLDAAFLGTARVVGWLGLMFGTFIYNRYLQDMTLRKSLLFAHIGLSVTILLDMVLVSR 383

Query: 378 VYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQLCPPGCEGSLMAFVMSAIALAFI 427
                GV D   V+  S L + +   K +PF +L  +LCPPG EG+L A  MS   L   
Sbjct: 384 ANVGYGVSDKTMVLFGSALGDAINQLKFMPFLILSGRLCPPGIEGTLFALFMSINNLGNT 443

BLAST of Carg25479 vs. TAIR 10
Match: AT2G32040.1 (Major facilitator superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.4e-41
Identity = 122/430 (28.37%), Postives = 213/430 (49.53%), Query Frame = 0

Query: 8   NPIRKVLGLGYWVQGFRCFPWMAVNFFLKDGLNVDPSTLQLLQNSANLPMVAKPLYGILS 67
           +P    + + Y+VQG      +AV+F+LKD L++DP+   ++   ++LP + KPLYG +S
Sbjct: 124 SPDNVAVAMVYFVQGVLGLARLAVSFYLKDDLHLDPAETAVITGLSSLPWLVKPLYGFIS 183

Query: 68  DAVYIFGQHRIPYIAIGALLQAVSWIPIATMSPSSISVFTISIYLLLSNLGASIAEVAND 127
           D+V +FG  R  Y+ +  LL A SW   + M+    S ++ +  +LL +L  + ++V  D
Sbjct: 184 DSVPLFGYRRRSYLVLSGLLGAFSW---SLMAGFVDSKYSAAFCILLGSLSVAFSDVVVD 243

Query: 128 AIVAEAGKQPTSSKNSRASSSGELQAFVWVASSAGGILGNLLGGIGIDRFSPQEMFLFFG 187
           ++V E       ++    S SG LQ+  W +S+ GGI+ +   G  ++ +  + +F    
Sbjct: 244 SMVVE------RARGESQSVSGSLQSLCWGSSAFGGIVSSYFSGSLVESYGVRFVFGVTA 303

Query: 188 LLLLLQFLLTIIVRERSLNLPQSSTSAGI-----------KKQLSQLFVVLKRPEIVYSI 247
           LL L+   + ++V E+ +  P S     I           K+ + QL+  +K+P  V+  
Sbjct: 304 LLPLITSAVAVLVNEQRVVRPASGQKENITLLSPGFLQTSKQNMIQLWGAIKQPN-VFLP 363

Query: 248 TWFAASYSIIPALTGTMFFYQTEHLKIESSVLGISKVIGQAALLLWGVIYNRHLKSIPSR 307
           T F   +   P     MF++ T  L      LG  K++   A LL   +YN  LK++P R
Sbjct: 364 TLFIFLWQATPHSDSAMFYFTTNKLGFTPEFLGRVKLVTSIASLLGVGLYNGFLKTVPLR 423

Query: 308 KLISSIQVLMAVFMISDMLFVKGVYREMGVPDFVYVVLFSGLSEVLLFFKILPFSVLIAQ 367
           K+     +      ++ ++ V G  R++G+ D  + +  S +  VL     +P  VL A+
Sbjct: 424 KIFLVTTIFGTGLGMTQVILVSGFNRQLGISDEWFAIGDSLILTVLAQASFMPVLVLAAR 483

Query: 368 LCPPGCEGSLMAFVMSAIALAFIVSGCLGVALASYVGVTANEFSGLPNGLIIQAVCTVLP 427
           LCP G E +L A +MS      ++ G +G  L    G+T + F  L   +I+  + ++LP
Sbjct: 484 LCPEGMEATLFATLMSISNGGSVLGGLMGAGLTQAFGITRDSFGNLSTLIILCNLSSLLP 543