Carg25477 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25477
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	cytochrome P450 89A2-like
Location	Carg_Chr20: 5249965 .. 5251395 (-)
RNA-Seq Expression	Carg25477
Synteny	Carg25477

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAACTACATCTCTTCCAATTCTCATCATAGCCATCATCTCCATCCTCACAATCTTCATGCTCATCCGCCATGGCCGCTCCTTCAAGAACCTTCCACCAGGGCCATCCCCTCTCCCATTTCTGGGCAACATCTTCCTCCTCCGGAAACACTCCTCATCATTCACATCGCTCATTCACACCCTCCATGCTCACTATGGCCCCATCTTCACACTCCACTACGGCTCCCGCCCTGTCATCTTCATCGCCGCCCATTCCATCGCCCACCAAGCTCTCAACCACAACGGCGCTGCATTCGCTGGCCGGCCCCCTGCCTCCACCACTACTAAGATCCTCTACAGCAACCAAATCACCATCTTCAACGCCTTCCACGGCCCCAACTGGAACCTCCTCCGTCGTAACCTCGTCTCTGAAATCCTCCACCCCTCTCGCCTGAAACTCCACTCCGACACCCACCGGTGGAGCCTGCACTTGCTAGTCTCTCGCCTGAAATCGAACTCCCAGGGCGGCGCCACTGCCGTTCGCCTTGTCGACCATTTCTGGCCGGCTATGATGGAGTTATCAGTGTTCATGTGCTTTGGATTGAAGATCGACGAGGATCGAATCAAAGCCCTAGCCGATCTCCTCCATCGCGTTCTGTTGTACTCAAGTAGCGAAAACAAACTCAATCAGTATCCATTAAAATTAGGGAAATTCCTCTGCCCTAATCTCTGGAAGGAACTCTCAACACTTCGAAACGAAATCGAAACTGCAATAATTCCTCTAATTAAATCCAGATTCCACGAAAGACAACGCGAAATCGAACAAAAGAAGAACGAATTAGAATCAGAATCAGAAACAAAATCAGAACAGATTTCGCCCTATCTGGACACATTGATAAACCTACACCTCCCAGAAGAGAATCGAAAGCTCGAAGAAGGTGAAATCATCAGCCTATGCGTGGAATTTCTCAACGGAATCGTCCATTCCACAGTAGTGGTGATGGAATGGGCAATGGCAAACATCGTAAAATATCCAGAAATCCAAGAGAAGGTATATAGAGAGATGAATGAGGCGATGGATGGAGCATCAACGAAAGACTACATAAGAGAGGAGGAGTTGAAGAAGATACCGTACCTGAAAGCAGTGATAATGGAGACCTTGAGGAGGCATCCGCCAGGGCATTTGGCTCTACCACATACAGTGACAGAGGAGGTGGTATTGGACGGGAGATTCGTGGTGCCGGAGAAAACGACAGTGCATCTAGCGGTGGCGGAGATGGCGAGGAACGAGGAGGTATGGGAGGAGGCGATGGAGTTCCGGCCGGAGAGATTCTTGAGAAACGAGGAAGAGGAGGCGGAAGGTAGAGAAGAGATTTGTAGGGGGAGGAGGGAGATTAAGTTGATGCCATATGGGGGAGGGAATACACTTATCTCGACTCATAAAGCGTAA

mRNA sequence

ATGGAAACTACATCTCTTCCAATTCTCATCATAGCCATCATCTCCATCCTCACAATCTTCATGCTCATCCGCCATGGCCGCTCCTTCAAGAACCTTCCACCAGGGCCATCCCCTCTCCCATTTCTGGGCAACATCTTCCTCCTCCGGAAACACTCCTCATCATTCACATCGCTCATTCACACCCTCCATGCTCACTATGGCCCCATCTTCACACTCCACTACGGCTCCCGCCCTGTCATCTTCATCGCCGCCCATTCCATCGCCCACCAAGCTCTCAACCACAACGGCGCTGCATTCGCTGGCCGGCCCCCTGCCTCCACCACTACTAAGATCCTCTACAGCAACCAAATCACCATCTTCAACGCCTTCCACGGCCCCAACTGGAACCTCCTCCGTCGTAACCTCGTCTCTGAAATCCTCCACCCCTCTCGCCTGAAACTCCACTCCGACACCCACCGGTGGAGCCTGCACTTGCTAGTCTCTCGCCTGAAATCGAACTCCCAGGGCGGCGCCACTGCCGTTCGCCTTGTCGACCATTTCTGGCCGGCTATGATGGAGTTATCAGTGTTCATGTGCTTTGGATTGAAGATCGACGAGGATCGAATCAAAGCCCTAGCCGATCTCCTCCATCGCGTTCTGTTGTACTCAAGTAGCGAAAACAAACTCAATCAGTATCCATTAAAATTAGGGAAATTCCTCTGCCCTAATCTCTGGAAGGAACTCTCAACACTTCGAAACGAAATCGAAACTGCAATAATTCCTCTAATTAAATCCAGATTCCACGAAAGACAACGCGAAATCGAACAAAAGAAGAACGAATTAGAATCAGAATCAGAAACAAAATCAGAACAGATTTCGCCCTATCTGGACACATTGATAAACCTACACCTCCCAGAAGAGAATCGAAAGCTCGAAGAAGGTGAAATCATCAGCCTATGCGTGGAATTTCTCAACGGAATCGTCCATTCCACAGTAGTGGTGATGGAATGGGCAATGGCAAACATCGTAAAATATCCAGAAATCCAAGAGAAGGTATATAGAGAGATGAATGAGGCGATGGATGGAGCATCAACGAAAGACTACATAAGAGAGGAGGAGTTGAAGAAGATACCGTACCTGAAAGCAGTGATAATGGAGACCTTGAGGAGGCATCCGCCAGGGCATTTGGCTCTACCACATACAGTGACAGAGGAGGTGGTATTGGACGGGAGATTCGTGGTGCCGGAGAAAACGACAGTGCATCTAGCGGTGGCGGAGATGGCGAGGAACGAGGAGGTATGGGAGGAGGCGATGGAGTTCCGGCCGGAGAGATTCTTGAGAAACGAGGAAGAGGAGGCGGAAGGTAGAGAAGAGATTTGTAGGGGGAGGAGGGAGATTAAGTTGATGCCATATGGGGGAGGGAATACACTTATCTCGACTCATAAAGCGTAA

Coding sequence (CDS)

ATGGAAACTACATCTCTTCCAATTCTCATCATAGCCATCATCTCCATCCTCACAATCTTCATGCTCATCCGCCATGGCCGCTCCTTCAAGAACCTTCCACCAGGGCCATCCCCTCTCCCATTTCTGGGCAACATCTTCCTCCTCCGGAAACACTCCTCATCATTCACATCGCTCATTCACACCCTCCATGCTCACTATGGCCCCATCTTCACACTCCACTACGGCTCCCGCCCTGTCATCTTCATCGCCGCCCATTCCATCGCCCACCAAGCTCTCAACCACAACGGCGCTGCATTCGCTGGCCGGCCCCCTGCCTCCACCACTACTAAGATCCTCTACAGCAACCAAATCACCATCTTCAACGCCTTCCACGGCCCCAACTGGAACCTCCTCCGTCGTAACCTCGTCTCTGAAATCCTCCACCCCTCTCGCCTGAAACTCCACTCCGACACCCACCGGTGGAGCCTGCACTTGCTAGTCTCTCGCCTGAAATCGAACTCCCAGGGCGGCGCCACTGCCGTTCGCCTTGTCGACCATTTCTGGCCGGCTATGATGGAGTTATCAGTGTTCATGTGCTTTGGATTGAAGATCGACGAGGATCGAATCAAAGCCCTAGCCGATCTCCTCCATCGCGTTCTGTTGTACTCAAGTAGCGAAAACAAACTCAATCAGTATCCATTAAAATTAGGGAAATTCCTCTGCCCTAATCTCTGGAAGGAACTCTCAACACTTCGAAACGAAATCGAAACTGCAATAATTCCTCTAATTAAATCCAGATTCCACGAAAGACAACGCGAAATCGAACAAAAGAAGAACGAATTAGAATCAGAATCAGAAACAAAATCAGAACAGATTTCGCCCTATCTGGACACATTGATAAACCTACACCTCCCAGAAGAGAATCGAAAGCTCGAAGAAGGTGAAATCATCAGCCTATGCGTGGAATTTCTCAACGGAATCGTCCATTCCACAGTAGTGGTGATGGAATGGGCAATGGCAAACATCGTAAAATATCCAGAAATCCAAGAGAAGGTATATAGAGAGATGAATGAGGCGATGGATGGAGCATCAACGAAAGACTACATAAGAGAGGAGGAGTTGAAGAAGATACCGTACCTGAAAGCAGTGATAATGGAGACCTTGAGGAGGCATCCGCCAGGGCATTTGGCTCTACCACATACAGTGACAGAGGAGGTGGTATTGGACGGGAGATTCGTGGTGCCGGAGAAAACGACAGTGCATCTAGCGGTGGCGGAGATGGCGAGGAACGAGGAGGTATGGGAGGAGGCGATGGAGTTCCGGCCGGAGAGATTCTTGAGAAACGAGGAAGAGGAGGCGGAAGGTAGAGAAGAGATTTGTAGGGGGAGGAGGGAGATTAAGTTGATGCCATATGGGGGAGGGAATACACTTATCTCGACTCATAAAGCGTAA

Protein sequence

METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA

Homology

[Close]

BLAST of Carg25477 vs. NCBI nr
Match: KAG7011013.1 (Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 941.8 bits (2433), Expect = 2.3e-270
Identity = 476/476 (100.00%), Postives = 476/476 (100.00%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH
Sbjct: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF
Sbjct: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF
Sbjct: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE
Sbjct: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE
Sbjct: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD
Sbjct: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM
Sbjct: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA 477
           ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA
Sbjct: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA 476

[Close]

BLAST of Carg25477 vs. NCBI nr
Match: KAG6571210.1 (Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 916.0 bits (2366), Expect = 1.3e-262
Identity = 464/476 (97.48%), Postives = 470/476 (98.74%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           METTSLPILIIAIISILTIF+LIRHG SFKNLPPGPSPLPFLGNIFLLRKHSSSF+SLIH
Sbjct: 1   METTSLPILIIAIISILTIFILIRHGCSFKNLPPGPSPLPFLGNIFLLRKHSSSFSSLIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLHAHYGPIFTLH+GSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF
Sbjct: 61  TLHAHYGPIFTLHFGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNS+GGATA+RLVDHF
Sbjct: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSKGGATAIRLVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE
Sbjct: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL    ETKSEQISPYLDTLINLHLPEE
Sbjct: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL----ETKSEQISPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEK+YREMNEAMDGASTKD
Sbjct: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLYREMNEAMDGASTKD 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM
Sbjct: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA 477
           ARNEEVWEEAMEFR ERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA
Sbjct: 421 ARNEEVWEEAMEFRAERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTLISTHKA 472

[Close]

BLAST of Carg25477 vs. NCBI nr
Match: XP_022944045.1 (cytochrome P450 89A2-like [Cucurbita moschata])

HSP 1 Score: 888.3 bits (2294), Expect = 3.0e-254
Identity = 452/470 (96.17%), Postives = 458/470 (97.45%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           METTSLPILIIAIISILTIF+LIRHG SFKNLPPGP PLPFLGNIFLLRKHSSSF+SLIH
Sbjct: 1   METTSLPILIIAIISILTIFILIRHGCSFKNLPPGPYPLPFLGNIFLLRKHSSSFSSLIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLHAHYGPIFTLH+GSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF
Sbjct: 61  TLHAHYGPIFTLHFGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKS+SQGGATAVRLVDHF
Sbjct: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSHSQGGATAVRLVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKIDEDRIKALADLLHRVLLYSS ENKLNQYPLKLGKFLCPNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSRENKLNQYPLKLGKFLCPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL    ETKSEQISPYLDTLINLHLPEE
Sbjct: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL----ETKSEQISPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEK+YREMNEAMDGASTKD
Sbjct: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLYREMNEAMDGASTKD 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM
Sbjct: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARNEEVWEEAMEFR ERFLRNEEEEAEGREEI RGRREIKLMPYGGG  +
Sbjct: 421 ARNEEVWEEAMEFRAERFLRNEEEEAEGREEIWRGRREIKLMPYGGGRRM 466

[Close]

BLAST of Carg25477 vs. NCBI nr
Match: XP_023513260.1 (cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 871.3 bits (2250), Expect = 3.8e-249
Identity = 443/470 (94.26%), Postives = 451/470 (95.96%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           METTSLPILII IISILTIFMLIRH RSF NLPPGPSPLPFLGNIFLLRKHSSSF+SLIH
Sbjct: 1   METTSLPILIITIISILTIFMLIRHARSFNNLPPGPSPLPFLGNIFLLRKHSSSFSSLIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLHAHYGPIFTLH+GSRPVIFIAAHSIAHQALNHNGAA AGRPPASTTTKILYSNQITIF
Sbjct: 61  TLHAHYGPIFTLHFGSRPVIFIAAHSIAHQALNHNGAALAGRPPASTTTKILYSNQITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           NAFHGPNWNLLRRNLVSEILHPSRLKLHSDT RWSLHLLVSRLKS+SQGGATAVRLVDHF
Sbjct: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTRRWSLHLLVSRLKSHSQGGATAVRLVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNEIETAIIPLIKSRFHERQ EIEQK NELESESETKSEQISPYLDTLINLHLPEE
Sbjct: 241 LSTLRNEIETAIIPLIKSRFHERQSEIEQKNNELESESETKSEQISPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEK+YREMNEAMDGASTKD
Sbjct: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLYREMNEAMDGASTKD 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           YIRE ++KK+PYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM
Sbjct: 361 YIREGDVKKMPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARNEEVWEEAMEFRPERFLRNEEEEAEGRE       E KLMPYGGG  +
Sbjct: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGRE-------ETKLMPYGGGRRM 463

[Close]

BLAST of Carg25477 vs. NCBI nr
Match: XP_008462791.1 (PREDICTED: cytochrome P450 89A2-like [Cucumis melo] >KAA0066308.1 cytochrome P450 89A2-like [Cucumis melo var. makuwa] >TYK00969.1 cytochrome P450 89A2-like [Cucumis melo var. makuwa])

HSP 1 Score: 745.7 bits (1924), Expect = 2.4e-211
Identity = 379/470 (80.64%), Postives = 412/470 (87.66%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME   L ILI   I I+TIF LIRH RS KNLPPGPSPLPFLGNI LLRKHSSSF SL+H
Sbjct: 1   MEIKWLSILITTTIFIITIFKLIRHARSLKNLPPGPSPLPFLGNILLLRKHSSSFFSLLH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLH  YGPIFTLH+ SRPVIFIA HS+AHQAL H+GAAFA RPP STTTKILYSN ITIF
Sbjct: 61  TLHLQYGPIFTLHFASRPVIFIAGHSLAHQALLHHGAAFADRPPPSTTTKILYSNHITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
            AFHGPNWNLLRRNL+SEILHPSRLKLHSD  RWSL  LVSRLK +SQGG  AV  VDHF
Sbjct: 121 TAFHGPNWNLLRRNLISEILHPSRLKLHSDARRWSLLSLVSRLKLHSQGGTAAVCFVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKI+EDRIKALAD+LHRVLLYSSS+NKLNQYPLKLGKF+ PNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIEEDRIKALADVLHRVLLYSSSQNKLNQYPLKLGKFIHPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLR+E+ETAIIPLI++RF+ RQ EIEQ+KNE ESESETKSEQI PYLDTLINLHLPEE
Sbjct: 241 LSTLRDELETAIIPLIEARFNNRQGEIEQRKNEFESESETKSEQILPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           +RKLEEGEIISLC EFLNGIVHSTVVVMEWAMANIVKYPEIQEK++REMN+ M+  S+K+
Sbjct: 301 DRKLEEGEIISLCAEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLWREMNQVMNITSSKE 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
            I EE+LKK+PYLKAVIMETLRRHPPGHLALPH VTEEVVLDG+FVVPEKTTVH A AEM
Sbjct: 361 LITEEDLKKLPYLKAVIMETLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARN +VWEE MEFR ERFLR EEEE E  EEI  G++EI+ MP+GGG  +
Sbjct: 421 ARNGKVWEEPMEFRAERFLRKEEEEEE--EEILTGKKEIRFMPFGGGRRI 468

[Close]

BLAST of Carg25477 vs. ExPASy Swiss-Prot
Match: Q42602 (Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2)

HSP 1 Score: 332.0 bits (850), Expect = 1.1e-89
Identity = 193/465 (41.51%), Postives = 283/465 (60.86%), Query Frame = 0


Query: 8   ILIIAII--SILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAH 67
           +LI+A +  S+L   +L R   S   LPP P+ LPFLG +  LR+      S + ++H  
Sbjct: 5   LLILASLSGSLLLHLLLRRRNSSSPPLPPDPNFLPFLGTLQWLREGLGGLESYLRSVHHR 64

Query: 68  YGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHG 127
            GPI TL   SRP IF+A  S+ H+AL  NGA +A RPP +  +KI+  + I+  +  +G
Sbjct: 65  LGPIVTLRITSRPAIFVADRSLTHEALVLNGAVYADRPPPAVISKIVDEHNIS--SGSYG 124

Query: 128 PNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMM 187
             W LLRRN+ SEILHPSR++ +S    W L +L  R +++  GG   + L+ H   AM 
Sbjct: 125 ATWRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNH--GGEEPIVLIHHLHYAMF 184

Query: 188 ELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLR 247
            L V MCFG K+DE +IK +  +    LL  +  N  N +P K  K +    W+E   +R
Sbjct: 185 ALLVLMCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWP-KFTKLILRKRWQEFLQIR 244

Query: 248 NEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLE 307
            +    ++PLI++    R++ +E++K    SE E K + +  Y+DTL++L LPEENRKL 
Sbjct: 245 RQQRDVLLPLIRA----RRKIVEERK---RSEQEDKKDYVQSYVDTLLDLELPEENRKLN 304

Query: 308 EGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREE 367
           E +I++LC EFL     +T   ++W MAN+VKYPEIQE+++ E+   + G   K+ + EE
Sbjct: 305 EEDIMNLCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVV-GEEAKE-VEEE 364

Query: 368 ELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEE 427
           +++K+PYLKAV++E LRRHPPGH  LPH+VTE+ VL G + VP+  T++  VAE+ R+  
Sbjct: 365 DVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGG-YKVPKNGTINFMVAEIGRDPV 424

Query: 428 VWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
            WEE M F+PERF+  EEE  +       G R IK+MP+G G  +
Sbjct: 425 EWEEPMAFKPERFM-GEEEAVD-----LTGSRGIKMMPFGAGRRI 448

[Close]

BLAST of Carg25477 vs. ExPASy Swiss-Prot
Match: Q9SRQ1 (Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 7.8e-88
Identity = 191/478 (39.96%), Postives = 275/478 (57.53%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHS-SSFTSLI 60
           ME T++  LII+ ++  +IF+ +    S   LPPGP   P +GNI  L+K++ S F  ++
Sbjct: 1   MEITTIIFLIISSLT-FSIFLKLIFFFSTHKLPPGPPRFPVIGNIIWLKKNNFSDFQGVL 60

Query: 61  HTLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITI 120
             L + +GPI TLH GS+P I++   S+AHQAL  NGA F+ R  A  TTK++ SNQ  I
Sbjct: 61  RDLASRHGPIITLHVGSKPSIWVTDRSLAHQALVQNGAVFSDRSLALPTTKVITSNQHDI 120

Query: 121 FNAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDH 180
            ++ +G  W  LRRNL SEIL PSR+K H+ + +WSL +LV   ++  +        +DH
Sbjct: 121 HSSVYGSLWRTLRRNLTSEILQPSRVKAHAPSRKWSLEILVDLFETEQREKGHISDALDH 180

Query: 181 FWPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWK 240
              AM  L   MCFG K+ ++ I+ + +  +++L+  +  + LN +P  + KFL    WK
Sbjct: 181 LRHAMFYLLALMCFGEKLRKEEIREIEEAQYQMLISYTKFSVLNIFP-SVTKFLLRRKWK 240

Query: 241 ELSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPE 300
           E   LR   E+ I+  + +R                  S+  +  +  Y+DTL+NL +P 
Sbjct: 241 EFLELRKSQESVILRYVNAR------------------SKETTGDVLCYVDTLLNLEIPT 300

Query: 301 E------NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAM 360
           E       RKL + EI+SLC EFLN     T   M+W MA +VKYPEIQ KVY EM    
Sbjct: 301 EEKEGGKKRKLSDSEIVSLCSEFLNAATDPTATSMQWIMAIMVKYPEIQRKVYEEMKTVF 360

Query: 361 DG-ASTKDYIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTT 420
            G    ++ IREE+L K+ YLKAVI+E LRRHPPGH    H VT + VL G F++P + T
Sbjct: 361 AGEEEEREEIREEDLGKLSYLKAVILECLRRHPPGHYLSYHKVTHDTVLGG-FLIPRQGT 420

Query: 421 VHLAVAEMARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ++  V EM R+ ++WE+ + F+PERFL N     E  +    G REIK+MP+G G  +
Sbjct: 421 INFMVGEMGRDPKIWEDPLTFKPERFLEN----GEACDFDMTGTREIKMMPFGAGRRM 453

[Close]

BLAST of Carg25477 vs. ExPASy Swiss-Prot
Match: O48928 (Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1)

HSP 1 Score: 228.0 bits (580), Expect = 2.2e-58
Identity = 139/453 (30.68%), Postives = 234/453 (51.66%), Query Frame = 0


Query: 19  IFMLIRHGRSFK-NLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAHYGPIFTLHYGSR 78
           IF L +  +S K NLPPGP   P +GN+F + +    F   ++ +   YG IFTL  G+R
Sbjct: 23  IFFLKQKSKSKKFNLPPGPPGWPIVGNLFQVARSGKPFFEYVNDVRLKYGSIFTLKMGTR 82

Query: 79  PVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHGPNWNLLRRNLVS 138
            +I +    + H+A+   GA +A RPP + T  I   N+ T+  A +GP W  LRRN+V 
Sbjct: 83  TMIILTDAKLVHEAMIQKGATYATRPPENPTRTIFSENKFTVNAATYGPVWKSLRRNMVQ 142

Query: 139 EILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMMELSVFMCFGLKI 198
            +L  +RLK        ++  L++RLK  ++     V ++     A+  + V MCFGL++
Sbjct: 143 NMLSSTRLKEFRSVRDNAMDKLINRLKDEAEKNNGVVWVLKDARFAVFCILVAMCFGLEM 202

Query: 199 DEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLRNEIETAIIPLIK 258
           DE+ ++ +  ++  VL+  + + +++ Y   L  F      K L   R ++E  ++P+  
Sbjct: 203 DEETVERIDQVMKSVLI--TLDPRIDDYLPILSPFFSKQRKKALEVRREQVE-FLVPI-- 262

Query: 259 SRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLEEGEIISLCVEFL 318
                    IEQ++  +++     +     YLDTL +L +  +     + E++SLC EFL
Sbjct: 263 ---------IEQRRRAIQNPGSDHTATTFSYLDTLFDLKVEGKKSAPSDAELVSLCSEFL 322

Query: 319 NGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREEELKKIPYLKAVI 378
           NG   +T   +EW +A ++  P +Q K+Y E+   +     +  + E++++K+PYL AV+
Sbjct: 323 NGGTDTTATAVEWGIAQLIANPNVQTKLYEEIKRTVG----EKKVDEKDVEKMPYLHAVV 382

Query: 379 METLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEEVWEEAMEFRPER 438
            E LR+HPP H  L H VTE   L G + +P    V +    +A + + W    +F PER
Sbjct: 383 KELLRKHPPTHFVLTHAVTEPTTLGG-YDIPIDANVEVYTPAIAEDPKNWLNPEKFDPER 442

Query: 439 FLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           F+      + G E    G   +K+MP+G G  +
Sbjct: 443 FI------SGGEEADITGVTGVKMMPFGVGRRI 450

[Close]

BLAST of Carg25477 vs. ExPASy Swiss-Prot
Match: P37123 (Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=1)

HSP 1 Score: 223.4 bits (568), Expect = 5.4e-57
Identity = 143/462 (30.95%), Postives = 244/462 (52.81%), Query Frame = 0


Query: 12  AIISILTIFMLIRHGRS-FKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAHYGPIF 71
           +++  L IF+L R  +S   NLPPGP   P +GN+F +      F   I  L   YG IF
Sbjct: 6   SLLFSLFIFLLTRKPKSKTPNLPPGPPGWPIVGNLFQVAGSGKQFFEYIRDLKPKYGSIF 65

Query: 72  TLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHGPNWNL 131
           TL  GSR +I +A+  +AH+AL   G  FA RP  + T  I   N+ ++  A +GP W  
Sbjct: 66  TLKMGSRTMIIVASAELAHEALIQKGQIFASRPRENPTRTIFSCNKFSVNAAVYGPVWRS 125

Query: 132 LRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMMELSVF 191
           LRRN+V  +L PSRLK   +    ++  L+ R++ +++     V  + +   A+  + V 
Sbjct: 126 LRRNMVQNMLSPSRLKEFREFREIAMDKLIERIRVDAKENNDVVWALKNARFAVFYILVA 185

Query: 192 MCFGLKID-EDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLRNEIE 251
           MCFG+++D E+ I+ +  ++  VL+    + +++ +   L  F+     K ++ +R    
Sbjct: 186 MCFGVEMDNEEMIERVDQMMKDVLIV--LDPRIDDFLPILRLFVGYKQRKRVNEVRKRQI 245

Query: 252 TAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLEEGEI 311
             ++PL           IE++++ +++    K+     YLDTL ++ +          E+
Sbjct: 246 ETLVPL-----------IEKRRSVVQNPGSDKTAASFSYLDTLFDVKVEGRKSGPTNAEL 305

Query: 312 ISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREEELKK 371
           ++LC EFLNG   +T   +EW +  +++ P IQ ++Y+E+   +        + E +++K
Sbjct: 306 VTLCSEFLNGGTDTTATALEWGIGRLMENPTIQNQLYQEIKTIVGDKK----VDENDIEK 365

Query: 372 IPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEEVWEE 431
           +PYL AV+ E LR+HPP +  L H+VTE V L G + +P  T V   V  ++ +  VW +
Sbjct: 366 MPYLNAVVKELLRKHPPTYFTLTHSVTEPVKLAG-YDIPMDTNVEFFVHGISHDPNVWSD 425

Query: 432 AMEFRPERFLRNEEEEAEGREEI-CRGRREIKLMPYGGGNTL 471
             +F P+RFL        GRE+    G +E+K+MP+G G  +
Sbjct: 426 PEKFDPDRFL-------SGREDADITGVKEVKMMPFGVGRRI 442

[Close]

BLAST of Carg25477 vs. ExPASy Swiss-Prot
Match: Q9LZ31 (Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 8.1e-53
Identity = 134/470 (28.51%), Postives = 236/470 (50.21%), Query Frame = 0


Query: 4   TSLPILIIAIISILTIFMLIRHGRSFK---NLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 63
           TSL     AII    +F++ R   + K   NLPPGP   P +GN+F   +    F     
Sbjct: 10  TSLDFTFFAIIISGFVFIITRWNSNSKKRLNLPPGPPGWPVVGNLFQFARSGKPFFEYAE 69

Query: 64  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 123
            L   YGPIFTL  G+R +I ++  ++ H+AL   GA FA RP  + T  I   N+ T+ 
Sbjct: 70  DLKKTYGPIFTLRMGTRTMIILSDATLVHEALIQRGALFASRPAENPTRTIFSCNKFTVN 129

Query: 124 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 183
            A +GP W  LRRN+V  +L  +RLK      + ++  L+ R+KS ++     + ++ + 
Sbjct: 130 AAKYGPVWRSLRRNMVQNMLSSTRLKEFGKLRQSAMDKLIERIKSEARDNDGLIWVLKNA 189

Query: 184 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 243
             A   + + MCFG+++DE+ I+ + ++L  VL+  + + +++ Y   L  F      + 
Sbjct: 190 RFAAFCILLEMCFGIEMDEETIEKMDEILKTVLM--TVDPRIDDYLPILAPFFSKERKRA 249

Query: 244 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 303
           L   R +++  +              IE+++  +++    K+     YLDTL +L +   
Sbjct: 250 LEVRREQVDYVV------------GVIERRRRAIQNPGSDKTASSFSYLDTLFDLKIEGR 309

Query: 304 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 363
                  E+++LC EFLNG   +T   +EW +A ++  PEIQ ++Y E+   +      D
Sbjct: 310 KTTPSNEELVTLCSEFLNGGTDTTGTAIEWGIAQLIANPEIQSRLYDEIKSTVGDDRRVD 369

Query: 364 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 423
              E+++ K+ +L+A + E LR+HPP + +L H V E   L G + +P    V + +  +
Sbjct: 370 ---EKDVDKMVFLQAFVKELLRKHPPTYFSLTHAVMETTTLAG-YDIPAGVNVEVYLPGI 429

Query: 424 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           + +  +W    +F P+RF+  +E+          G   +K++P+G G  +
Sbjct: 430 SEDPRIWNNPKKFDPDRFMLGKEDAD------ITGISGVKMIPFGVGRRI 455

[Close]

BLAST of Carg25477 vs. ExPASy TrEMBL
Match: A0A6J1FYF7 (cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448595 PE=3 SV=1)

HSP 1 Score: 888.3 bits (2294), Expect = 1.5e-254
Identity = 452/470 (96.17%), Postives = 458/470 (97.45%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           METTSLPILIIAIISILTIF+LIRHG SFKNLPPGP PLPFLGNIFLLRKHSSSF+SLIH
Sbjct: 1   METTSLPILIIAIISILTIFILIRHGCSFKNLPPGPYPLPFLGNIFLLRKHSSSFSSLIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLHAHYGPIFTLH+GSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF
Sbjct: 61  TLHAHYGPIFTLHFGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKS+SQGGATAVRLVDHF
Sbjct: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSHSQGGATAVRLVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKIDEDRIKALADLLHRVLLYSS ENKLNQYPLKLGKFLCPNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSRENKLNQYPLKLGKFLCPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL    ETKSEQISPYLDTLINLHLPEE
Sbjct: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNEL----ETKSEQISPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEK+YREMNEAMDGASTKD
Sbjct: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLYREMNEAMDGASTKD 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM
Sbjct: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARNEEVWEEAMEFR ERFLRNEEEEAEGREEI RGRREIKLMPYGGG  +
Sbjct: 421 ARNEEVWEEAMEFRAERFLRNEEEEAEGREEIWRGRREIKLMPYGGGRRM 466

[Close]

BLAST of Carg25477 vs. ExPASy TrEMBL
Match: A0A5A7VF17 (Cytochrome P450 89A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001530 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.2e-211
Identity = 379/470 (80.64%), Postives = 412/470 (87.66%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME   L ILI   I I+TIF LIRH RS KNLPPGPSPLPFLGNI LLRKHSSSF SL+H
Sbjct: 1   MEIKWLSILITTTIFIITIFKLIRHARSLKNLPPGPSPLPFLGNILLLRKHSSSFFSLLH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLH  YGPIFTLH+ SRPVIFIA HS+AHQAL H+GAAFA RPP STTTKILYSN ITIF
Sbjct: 61  TLHLQYGPIFTLHFASRPVIFIAGHSLAHQALLHHGAAFADRPPPSTTTKILYSNHITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
            AFHGPNWNLLRRNL+SEILHPSRLKLHSD  RWSL  LVSRLK +SQGG  AV  VDHF
Sbjct: 121 TAFHGPNWNLLRRNLISEILHPSRLKLHSDARRWSLLSLVSRLKLHSQGGTAAVCFVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKI+EDRIKALAD+LHRVLLYSSS+NKLNQYPLKLGKF+ PNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIEEDRIKALADVLHRVLLYSSSQNKLNQYPLKLGKFIHPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLR+E+ETAIIPLI++RF+ RQ EIEQ+KNE ESESETKSEQI PYLDTLINLHLPEE
Sbjct: 241 LSTLRDELETAIIPLIEARFNNRQGEIEQRKNEFESESETKSEQILPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           +RKLEEGEIISLC EFLNGIVHSTVVVMEWAMANIVKYPEIQEK++REMN+ M+  S+K+
Sbjct: 301 DRKLEEGEIISLCAEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLWREMNQVMNITSSKE 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
            I EE+LKK+PYLKAVIMETLRRHPPGHLALPH VTEEVVLDG+FVVPEKTTVH A AEM
Sbjct: 361 LITEEDLKKLPYLKAVIMETLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARN +VWEE MEFR ERFLR EEEE E  EEI  G++EI+ MP+GGG  +
Sbjct: 421 ARNGKVWEEPMEFRAERFLRKEEEEEE--EEILTGKKEIRFMPFGGGRRI 468

[Close]

BLAST of Carg25477 vs. ExPASy TrEMBL
Match: A0A1S3CI93 (cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103501081 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 1.2e-211
Identity = 379/470 (80.64%), Postives = 412/470 (87.66%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME   L ILI   I I+TIF LIRH RS KNLPPGPSPLPFLGNI LLRKHSSSF SL+H
Sbjct: 1   MEIKWLSILITTTIFIITIFKLIRHARSLKNLPPGPSPLPFLGNILLLRKHSSSFFSLLH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLH  YGPIFTLH+ SRPVIFIA HS+AHQAL H+GAAFA RPP STTTKILYSN ITIF
Sbjct: 61  TLHLQYGPIFTLHFASRPVIFIAGHSLAHQALLHHGAAFADRPPPSTTTKILYSNHITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
            AFHGPNWNLLRRNL+SEILHPSRLKLHSD  RWSL  LVSRLK +SQGG  AV  VDHF
Sbjct: 121 TAFHGPNWNLLRRNLISEILHPSRLKLHSDARRWSLLSLVSRLKLHSQGGTAAVCFVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKI+EDRIKALAD+LHRVLLYSSS+NKLNQYPLKLGKF+ PNLWKE
Sbjct: 181 WPTMMELSVFMCFGLKIEEDRIKALADVLHRVLLYSSSQNKLNQYPLKLGKFIHPNLWKE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLR+E+ETAIIPLI++RF+ RQ EIEQ+KNE ESESETKSEQI PYLDTLINLHLPEE
Sbjct: 241 LSTLRDELETAIIPLIEARFNNRQGEIEQRKNEFESESETKSEQILPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           +RKLEEGEIISLC EFLNGIVHSTVVVMEWAMANIVKYPEIQEK++REMN+ M+  S+K+
Sbjct: 301 DRKLEEGEIISLCAEFLNGIVHSTVVVMEWAMANIVKYPEIQEKLWREMNQVMNITSSKE 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
            I EE+LKK+PYLKAVIMETLRRHPPGHLALPH VTEEVVLDG+FVVPEKTTVH A AEM
Sbjct: 361 LITEEDLKKLPYLKAVIMETLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARN +VWEE MEFR ERFLR EEEE E  EEI  G++EI+ MP+GGG  +
Sbjct: 421 ARNGKVWEEPMEFRAERFLRKEEEEEE--EEILTGKKEIRFMPFGGGRRI 468

[Close]

BLAST of Carg25477 vs. ExPASy TrEMBL
Match: A0A0A0LQT2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G432220 PE=3 SV=1)

HSP 1 Score: 744.2 bits (1920), Expect = 3.4e-211
Identity = 376/470 (80.00%), Postives = 411/470 (87.45%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME   L ILI   I I+TIF LIRHGRS  NLPPGPSPLPFLGNI LLRKHSSSF SL+H
Sbjct: 1   MEIKWLSILITTTIFIITIFKLIRHGRSLNNLPPGPSPLPFLGNILLLRKHSSSFFSLLH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           TLH  YGPIFTLH+ SRPVIFIA HS+AHQAL H+GAAFA RPP S TTKILYSN ITIF
Sbjct: 61  TLHLQYGPIFTLHFASRPVIFIAGHSLAHQALLHHGAAFADRPPPSITTKILYSNHITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
            AFHGPNWNLLRRNL+SEILHPSRLKLHSD  RWSL  LVSRLK +SQGG  AV  VDHF
Sbjct: 121 TAFHGPNWNLLRRNLISEILHPSRLKLHSDARRWSLLSLVSRLKLHSQGGTAAVCFVDHF 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
           WP MMELSVFMCFGLKI+EDRIKALAD+LHRVLLYSSS+NKLNQYPLKLGKF+ PNLW +
Sbjct: 181 WPTMMELSVFMCFGLKIEEDRIKALADVLHRVLLYSSSQNKLNQYPLKLGKFIYPNLWNQ 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           LSTLRNE+ETAIIPLI++RF+ R+ + EQ+KNELESESETKSEQI PYLDTLINLHLPEE
Sbjct: 241 LSTLRNELETAIIPLIEARFNNRRADTEQRKNELESESETKSEQILPYLDTLINLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRKLEEGEIISLC EFLNGIVHSTVVVMEWAMANIVKYPE+QEK++REMN+ M+  S+K+
Sbjct: 301 NRKLEEGEIISLCAEFLNGIVHSTVVVMEWAMANIVKYPEVQEKLWREMNQVMNITSSKE 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
           +I EE+LKK+PYLKAVIMETLRRHPPGHLALPH VTEEVVLDG+FVVPEKTTVH A AEM
Sbjct: 361 HITEEDLKKLPYLKAVIMETLRRHPPGHLALPHLVTEEVVLDGKFVVPEKTTVHFAAAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARN EVWEE MEFR ERFLR EEEE E  EEI  G++EI+ MP+GGG  +
Sbjct: 421 ARNGEVWEEPMEFRAERFLRKEEEEEE--EEILSGKKEIRYMPFGGGRRI 468

[Close]

BLAST of Carg25477 vs. ExPASy TrEMBL
Match: A0A6J1KYV9 (cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111499502 PE=3 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 9.9e-195
Identity = 343/470 (72.98%), Postives = 402/470 (85.53%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME T LP+L I II I+TI  LIRHGRSF NLPPGP+PLPFLGNI LLRK+SSSF +LIH
Sbjct: 1   MEITWLPVLTITIIFIITILKLIRHGRSFPNLPPGPTPLPFLGNIILLRKYSSSFYALIH 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
            LH HYGPIFT ++GSR VIFIA HS+AHQAL H+GAA A RPPAS T KILYSN ITIF
Sbjct: 61  DLHVHYGPIFTFYFGSRRVIFIAGHSLAHQALAHHGAAIADRPPASITNKILYSNHITIF 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
            AFHGPNW LLRRNL+SE  HPS L+L SD  RWSL+ LVSRLKS+SQGGA AVRLVDH 
Sbjct: 121 TAFHGPNWGLLRRNLISETFHPSHLRLLSDARRWSLNSLVSRLKSHSQGGAAAVRLVDHI 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
              ++E+SVFMCFGLK++E+RIKALA+LLH+VLL+SS +NKLNQYPLKLGK L PNLW E
Sbjct: 181 RLTLVEISVFMCFGLKLEEERIKALAELLHQVLLHSSRQNKLNQYPLKLGKILYPNLWNE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
           +ST+RN++ETAIIPLIK+R  ERQREIEQ+ NELESESET++E++ PYLDTL+NLHLPEE
Sbjct: 241 ISTIRNKMETAIIPLIKARLDERQREIEQRNNELESESETETERVLPYLDTLLNLHLPEE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
           NRK+EEGEIISLC EFLNGIVH+T++VM+WAMANIVKYPEIQEKVY E+N+AMD +S+K+
Sbjct: 301 NRKMEEGEIISLCAEFLNGIVHTTLMVMQWAMANIVKYPEIQEKVYTEINQAMDQSSSKE 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
            + EE++KK+PYLKAVIMETLRRHPPG+L+LPH  T+EVVLDG+FVVP+KTTVH   AEM
Sbjct: 361 QVTEEDIKKMPYLKAVIMETLRRHPPGYLSLPHLATKEVVLDGKFVVPKKTTVHFVAAEM 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ARN EVW+E MEFRPERFL  E E+ E   E   GR+EI+ MP+GGG  +
Sbjct: 421 ARNGEVWKEPMEFRPERFLTKEAEKVE--VEALIGRKEIRFMPFGGGRRI 468

[Close]

BLAST of Carg25477 vs. TAIR 10
Match: AT2G12190.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 359.4 bits (921), Expect = 4.5e-99
Identity = 202/455 (44.40%), Postives = 277/455 (60.88%), Query Frame = 0


Query: 16  ILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAHYGPIFTLHYG 75
           +L + +  R   S   LPP P+  PFLG +  LR+      + + ++H   GPI TL   
Sbjct: 16  LLNLLLFRRRDSSSLPLPPDPNFFPFLGTLQWLRQGLGGLNNYLRSVHHRLGPIITLRIT 75

Query: 76  SRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHGPNWNLLRRNL 135
           SRP IF+A  S+AHQAL  NGA FA RPPA+  +KI+ SNQ  I ++ +G  W LLRRNL
Sbjct: 76  SRPAIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSSLYGATWRLLRRNL 135

Query: 136 VSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMMELSVFMCFGL 195
            SEILHPSR++ +S   RW L +L  R       G   + +VDH   AM  L V MCFG 
Sbjct: 136 TSEILHPSRVRSYSHARRWVLEILFDRF--GKSRGEEPIVVVDHLHYAMFALLVLMCFGD 195

Query: 196 KIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLRNEIETAIIPL 255
           K+DE +IK +  +  R LL  S  N LN +P K  K +    W+E   +R E    ++PL
Sbjct: 196 KLDEKQIKQVEYVQRRQLLGFSRFNILNLWP-KFTKLILRKRWEEFFQMRREQHDVLLPL 255

Query: 256 IKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLEEGEIISLCVE 315
           I++    R++ +E++KN    E E     +  Y+DTL+ L LP+E RKL E EI+SLC E
Sbjct: 256 IRA----RRKIVEERKNRSSEEEEDNKVYVQSYVDTLLELELPDEKRKLNEDEIVSLCSE 315

Query: 316 FLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREEELKKIPYLKA 375
           FLNG   +T   ++W MAN+VK PEIQ+++Y E+   + G   K+ + EE+ +K+PYLKA
Sbjct: 316 FLNGGTDTTATALQWIMANLVKNPEIQKRLYEEIKSVV-GEEAKE-VEEEDAQKMPYLKA 375

Query: 376 VIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEEVWEEAMEFRP 435
           V+ME LRRHPPGH  LPH+VTE+ VL G + VP+K T++  VAE+ R+  VWEE M F+P
Sbjct: 376 VVMEGLRRHPPGHFVLPHSVTEDTVLGG-YKVPKKGTINFMVAEIGRDPMVWEEPMAFKP 435

Query: 436 ERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           ERF+  EEE  +       G R IK+MP+G G  +
Sbjct: 436 ERFM-GEEEAVD-----ITGSRGIKMMPFGAGRRI 454

[Close]

BLAST of Carg25477 vs. TAIR 10
Match: AT1G64950.1 (cytochrome P450, family 89, subfamily A, polypeptide 5 )

HSP 1 Score: 354.8 bits (909), Expect = 1.1e-97
Identity = 199/452 (44.03%), Postives = 275/452 (60.84%), Query Frame = 0


Query: 19  IFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAHYGPIFTLHYGSRP 78
           +F  +R   S   LPP P+  PF+G I  LR+      + + ++H   GPI TL   SRP
Sbjct: 20  LFFRLRDSSSLP-LPPDPNYFPFIGTIQWLRQGLGGLNNYLRSVHHRLGPIITLRITSRP 79

Query: 79  VIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHGPNWNLLRRNLVSE 138
            IF+A  S+AHQAL  NGA FA RPPA+  +KI+ SNQ  I +  +G  W LLRRNL SE
Sbjct: 80  SIFVADRSLAHQALVLNGAVFADRPPAAPISKIISSNQHNISSCLYGATWRLLRRNLTSE 139

Query: 139 ILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMMELSVFMCFGLKID 198
           ILHPSR++ +S   RW L +L  R   N   G   + +VDH   AM  L V MCFG K+D
Sbjct: 140 ILHPSRVRSYSHARRWVLEILFDRFGKNR--GEEPIVVVDHLHYAMFALLVLMCFGDKLD 199

Query: 199 EDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLRNEIETAIIPLIKS 258
           E +IK +  +  R LL  S  N LN +P K  K +    W+E   +R E    ++PLI++
Sbjct: 200 EKQIKQVEYVQRRQLLGFSRFNILNLWP-KFTKLILRKRWEEFFQMRREQHDVLLPLIRA 259

Query: 259 RFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLEEGEIISLCVEFLN 318
               R++ +E++KN    E E   E +  Y+DTL+ L LP+E RKL E EI+SLC EFLN
Sbjct: 260 ----RRKIVEERKNRSSEEEEDNKEYVQSYVDTLLELELPDEKRKLNEDEIVSLCSEFLN 319

Query: 319 GIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREEELKKIPYLKAVIM 378
           G   +T   ++W MAN+VK P+IQ+++Y E+   +   + +  + EE+ +K+PYL+AV+M
Sbjct: 320 GGTDTTATALQWIMANLVKNPDIQKRLYEEIKSVVGEEANE--VEEEDAQKMPYLEAVVM 379

Query: 379 ETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEEVWEEAMEFRPERF 438
           E LRRHPPGH  LPH+VTE+ VL G + VP+  T++  VAE+ R+ +VWEE M F+PERF
Sbjct: 380 EGLRRHPPGHFVLPHSVTEDTVLGG-YKVPKNGTINFMVAEIGRDPKVWEEPMAFKPERF 439

Query: 439 LRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
           +    EEA        G R IK+MP+G G  +
Sbjct: 440 M----EEAVD----ITGSRGIKMMPFGAGRRI 452

[Close]

BLAST of Carg25477 vs. TAIR 10
Match: AT1G64940.1 (cytochrome P450, family 87, subfamily A, polypeptide 6 )

HSP 1 Score: 353.2 bits (905), Expect = 3.2e-97
Identity = 208/467 (44.54%), Postives = 287/467 (61.46%), Query Frame = 0


Query: 6   LPILIIAIISILTIFMLIRHGRSFKN--LPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLH 65
           L IL    +S+L   +L R   SF +  LPP P+  PF+G +  LRK      + + ++H
Sbjct: 5   LLILGSLFLSLLVNHLLFRRRDSFSSLPLPPDPNFFPFIGTLKWLRKGLGGLDNYLRSVH 64

Query: 66  AHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAF 125
            H GPI TL   SRP IF+   S+AHQAL  NGA FA RPPA + +KI+ SNQ  I +  
Sbjct: 65  HHLGPIITLRITSRPAIFVTDRSLAHQALVLNGAVFADRPPAESISKIISSNQHNISSCL 124

Query: 126 HGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPA 185
           +G  W LLRRNL SEILHPSRL+ +S   RW L +L  R   N   G   + +VDH   A
Sbjct: 125 YGATWRLLRRNLTSEILHPSRLRSYSHARRWVLEILFGRFGKNR--GEEPIVVVDHLHYA 184

Query: 186 MMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELST 245
           M  L V MCFG K+DE +IK +  +  R LL  S  N L  +P K  K +    W+E   
Sbjct: 185 MFALLVLMCFGDKLDEKQIKQVEYVQRRQLLGFSRFNILTLWP-KFTKLIYRKRWEEFFQ 244

Query: 246 LRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRK 305
           +++E +  ++PLI++R   R+   E+KK   E E + K E +  Y+DTL+++ LP+E RK
Sbjct: 245 MQSEQQDVLLPLIRAR---RKIVDERKKRSSEEEKDNK-EYVQSYVDTLLDVELPDEKRK 304

Query: 306 LEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIR 365
           L E EI+SLC EFLN    +T   ++W MAN+VK PEIQ ++Y E+ +++ G   K+ + 
Sbjct: 305 LNEDEIVSLCSEFLNAGTDTTATALQWIMANLVKNPEIQRRLYEEI-KSIVGEEAKE-VE 364

Query: 366 EEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARN 425
           E++ +K+PYLKAV+ME LRRHPPGH  LPH+VTE+ VL G + VP+K T++  VAE+ R+
Sbjct: 365 EQDAQKMPYLKAVVMEGLRRHPPGHFVLPHSVTEDTVLGG-YKVPKKGTINFMVAEIGRD 424

Query: 426 EEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
            +VWEE M F+PERF+    EEA        G R IK+MP+G G  +
Sbjct: 425 PKVWEEPMAFKPERFM----EEAVD----ITGSRGIKMMPFGAGRRI 453

[Close]

BLAST of Carg25477 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 334.3 bits (856), Expect = 1.6e-91
Identity = 196/470 (41.70%), Postives = 283/470 (60.21%), Query Frame = 0


Query: 1   METTSLPILIIAIISILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIH 60
           ME   L +  +++  +L +        S   LPP P+  PFLG +  LR+    F + + 
Sbjct: 1   MEIWLLILGSLSLSLLLNLLFFRLRDSSSLPLPPAPNFFPFLGTLQWLRQGLGGFNNYVR 60

Query: 61  TLHAHYGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIF 120
           ++H   GPI TL   SRP IF+A  S+AHQAL  NGA FA RPPA+  +KIL +NQ TI 
Sbjct: 61  SVHHRLGPIITLRITSRPAIFVADGSLAHQALVLNGAVFADRPPAAPISKILSNNQHTIT 120

Query: 121 NAFHGPNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHF 180
           +  +G  W LLRRN ++EILHPSR+K +S    W L +L  RL+ +  GG   + + DH 
Sbjct: 121 SCLYGVTWRLLRRN-ITEILHPSRMKSYSHVRHWVLEILFDRLRKS--GGEEPIVVFDHL 180

Query: 181 WPAMMELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKE 240
             AM  + V MCFG K+DE +IK +  +  ++LL  +  + LN  P K  K +    W+E
Sbjct: 181 HYAMFAVLVLMCFGDKLDEKQIKQVEYVQRQMLLGFARYSILNLCP-KFTKLILRKRWEE 240

Query: 241 LSTLRNEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEE 300
              +R E +  ++ LI    + R++ +E++K     E E   E +  Y+DTL+++ LP+E
Sbjct: 241 FFQMRREQQDVLLRLI----YARRKIVEERKKRSSEEEEENKEYVQSYVDTLLDVELPDE 300

Query: 301 NRKLEEGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKD 360
            RKL E EI+SLC EFL     +T  V++W MAN+VK  EIQE++Y E+   + G   K 
Sbjct: 301 KRKLNEDEIVSLCSEFLIAGSDTTATVLQWIMANLVKNQEIQERLYEEITNVV-GEEAK- 360

Query: 361 YIREEELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEM 420
            + E++ +K+PYLKAV+ME LRRHPPG+  LPH+VTE+ VL G + VP+K T++  VAE+
Sbjct: 361 VVEEKDTQKMPYLKAVVMEALRRHPPGNTVLPHSVTEDTVLGG-YKVPKKGTINFLVAEI 420

Query: 421 ARNEEVWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
            R+ +VWEE M F+PERF+  EEE  +       G R IK+MP+G G  +
Sbjct: 421 GRDPKVWEEPMAFKPERFM-GEEEAVD-----ITGSRGIKMMPFGAGRRI 453

[Close]

BLAST of Carg25477 vs. TAIR 10
Match: AT1G64900.1 (cytochrome P450, family 89, subfamily A, polypeptide 2 )

HSP 1 Score: 332.0 bits (850), Expect = 7.7e-91
Identity = 193/465 (41.51%), Postives = 283/465 (60.86%), Query Frame = 0


Query: 8   ILIIAII--SILTIFMLIRHGRSFKNLPPGPSPLPFLGNIFLLRKHSSSFTSLIHTLHAH 67
           +LI+A +  S+L   +L R   S   LPP P+ LPFLG +  LR+      S + ++H  
Sbjct: 5   LLILASLSGSLLLHLLLRRRNSSSPPLPPDPNFLPFLGTLQWLREGLGGLESYLRSVHHR 64

Query: 68  YGPIFTLHYGSRPVIFIAAHSIAHQALNHNGAAFAGRPPASTTTKILYSNQITIFNAFHG 127
            GPI TL   SRP IF+A  S+ H+AL  NGA +A RPP +  +KI+  + I+  +  +G
Sbjct: 65  LGPIVTLRITSRPAIFVADRSLTHEALVLNGAVYADRPPPAVISKIVDEHNIS--SGSYG 124

Query: 128 PNWNLLRRNLVSEILHPSRLKLHSDTHRWSLHLLVSRLKSNSQGGATAVRLVDHFWPAMM 187
             W LLRRN+ SEILHPSR++ +S    W L +L  R +++  GG   + L+ H   AM 
Sbjct: 125 ATWRLLRRNITSEILHPSRVRSYSHARHWVLEILFERFRNH--GGEEPIVLIHHLHYAMF 184

Query: 188 ELSVFMCFGLKIDEDRIKALADLLHRVLLYSSSENKLNQYPLKLGKFLCPNLWKELSTLR 247
            L V MCFG K+DE +IK +  +    LL  +  N  N +P K  K +    W+E   +R
Sbjct: 185 ALLVLMCFGDKLDEKQIKEVEFIQRLQLLSLTKFNIFNIWP-KFTKLILRKRWQEFLQIR 244

Query: 248 NEIETAIIPLIKSRFHERQREIEQKKNELESESETKSEQISPYLDTLINLHLPEENRKLE 307
            +    ++PLI++    R++ +E++K    SE E K + +  Y+DTL++L LPEENRKL 
Sbjct: 245 RQQRDVLLPLIRA----RRKIVEERK---RSEQEDKKDYVQSYVDTLLDLELPEENRKLN 304

Query: 308 EGEIISLCVEFLNGIVHSTVVVMEWAMANIVKYPEIQEKVYREMNEAMDGASTKDYIREE 367
           E +I++LC EFL     +T   ++W MAN+VKYPEIQE+++ E+   + G   K+ + EE
Sbjct: 305 EEDIMNLCSEFLTAGTDTTATALQWIMANLVKYPEIQERLHEEIKSVV-GEEAKE-VEEE 364

Query: 368 ELKKIPYLKAVIMETLRRHPPGHLALPHTVTEEVVLDGRFVVPEKTTVHLAVAEMARNEE 427
           +++K+PYLKAV++E LRRHPPGH  LPH+VTE+ VL G + VP+  T++  VAE+ R+  
Sbjct: 365 DVEKMPYLKAVVLEGLRRHPPGHFLLPHSVTEDTVLGG-YKVPKNGTINFMVAEIGRDPV 424

Query: 428 VWEEAMEFRPERFLRNEEEEAEGREEICRGRREIKLMPYGGGNTL 471
            WEE M F+PERF+  EEE  +       G R IK+MP+G G  +
Sbjct: 425 EWEEPMAFKPERFM-GEEEAVD-----LTGSRGIKMMPFGAGRRI 448

The following BLAST results are available for this feature:

BLAST of Carg25477 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011013.1	2.3e-270	100.00	Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6571210.1	1.3e-262	97.48	Cytochrome P450 89A9, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944045.1	3.0e-254	96.17	cytochrome P450 89A2-like [Cucurbita moschata]	[more]
XP_023513260.1	3.8e-249	94.26	cytochrome P450 89A2-like [Cucurbita pepo subsp. pepo]	[more]
XP_008462791.1	2.4e-211	80.64	PREDICTED: cytochrome P450 89A2-like [Cucumis melo] >KAA0066308.1 cytochrome P45...	[more]

BLAST of Carg25477 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42602	1.1e-89	41.51	Cytochrome P450 89A2 OS=Arabidopsis thaliana OX=3702 GN=CYP89A2 PE=2 SV=2	[more]
Q9SRQ1	7.8e-88	39.96	Cytochrome P450 89A9 OS=Arabidopsis thaliana OX=3702 GN=CYP89A9 PE=2 SV=1	[more]
O48928	2.2e-58	30.68	Cytochrome P450 77A3 OS=Glycine max OX=3847 GN=CYP77A3 PE=2 SV=1	[more]
P37123	5.4e-57	30.95	Cytochrome P450 77A1 (Fragment) OS=Solanum melongena OX=4111 GN=CYP77A1 PE=2 SV=...	[more]
Q9LZ31	8.1e-53	28.51	Cytochrome P450 77A4 OS=Arabidopsis thaliana OX=3702 GN=CYP77A4 PE=2 SV=1	[more]

BLAST of Carg25477 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FYF7	1.5e-254	96.17	cytochrome P450 89A2-like OS=Cucurbita moschata OX=3662 GN=LOC111448595 PE=3 SV=...	[more]
A0A5A7VF17	1.2e-211	80.64	Cytochrome P450 89A2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CI93	1.2e-211	80.64	cytochrome P450 89A2-like OS=Cucumis melo OX=3656 GN=LOC103501081 PE=3 SV=1	[more]
A0A0A0LQT2	3.4e-211	80.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G432220 PE=3 SV=1	[more]
A0A6J1KYV9	9.9e-195	72.98	cytochrome P450 89A2-like OS=Cucurbita maxima OX=3661 GN=LOC111499502 PE=3 SV=1	[more]

BLAST of Carg25477 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G12190.1	4.5e-99	44.40	Cytochrome P450 superfamily protein	[more]
AT1G64950.1	1.1e-97	44.03	cytochrome P450, family 89, subfamily A, polypeptide 5	[more]
AT1G64940.1	3.2e-97	44.54	cytochrome P450, family 87, subfamily A, polypeptide 6	[more]
AT1G64930.1	1.6e-91	41.70	cytochrome P450, family 87, subfamily A, polypeptide 7	[more]
AT1G64900.1	7.7e-91	41.51	cytochrome P450, family 89, subfamily A, polypeptide 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 257..284
None	No IPR available	PANTHER	PTHR24298:SF850	CYTOCHROME P450	coord: 9..470
None	No IPR available	PANTHER	PTHR24298	FLAVONOID 3'-MONOOXYGENASE-RELATED	coord: 9..470
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 87..108 score: 28.16 coord: 63..82 score: 34.92 coord: 416..440 score: 30.03 coord: 374..392 score: 35.52
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 21..469 e-value: 1.0E-79 score: 270.3
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 26..467
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 33..467 e-value: 3.0E-67 score: 227.4

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25477-RA	Carg25477-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016709	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen