Carg25475 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25475
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTocopherol O-methyltransferase
LocationCarg_Chr20: 5223202 .. 5226187 (-)
RNA-Seq ExpressionCarg25475
SyntenyCarg25475
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGAAAATCGCGTAGTTTTTTCTGTATCTTCTCTCACAGAATCTCTGCGCGGCTTTACTTTCCCAACGACTTGTCGTCGGCCATTGCTCCTTTCTTTATAAATTGTAAGCAGCAATGAGCTTCACCACTGCAACTCCAATCCTTACGCCTTCCTATTCCCTCTCCTCCCTCCATTCCAATCGCTTCAACGCCACAATCCTTTCTCCGATTCCTCTCCGTCGTTCCTTTTCGTCTTTCGCTCGTCGCCGCCGCAACTGCCCTCTGATTGCCGCTGCCGCGACGACGACGGACGTTGAGATCGATATGGATGTCGATAGCCTCAAGAAGGGAATCGCTGAGTTCTATGATGAATCGTCGGGGCTTTGGGAGAACGTTTGGGGTGAGCATATGCACCATGGTTTTTACGATCCTGATTCTTCCGTTTCTCTCTCTGATCACCGTGCCGCGCAAATCCGGATGATTGAGGAGACGCTCCGGTTTGCCGGTGTGGAGGCGGCGACGGCGGTGGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCGAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCCAAACGCGCTCAAGCAATTGCTGCTGATGCTGGATTAAGTGATAAGGTATGGACGTCGTTGAATCCTCTGTTCTTTTTTGTTCTTCTCCATTTATCTTGTTTTGTATTTTCTTCATTGAAGAATTTCCGTTACGCTTTTGTTTTACCGTTTTCTTTGTTTTGAACCAAACTTGAAAATTAGTCACGAATCAAGTAATACAACCAAAATAATGAATACCTGCTGTATTAGTCCTTTTCTTGTTTGGTTGCTGAAATTGTGAAAAGAGTGCCCACACAAACACTACTCCTCAAAATGTTTAGTACTTTTTTTGGGGTCCATTTTAAGATCTATTTTGACCTCTAATTGAATAACCGGATGATATAATTTATGAAATGATTCTTGAATTATGAACTCAAGAACATGCAAGGATGAAATGAATATCAAATTATGAATTCAAGAACATGTGAGGATGAAATGAACATCGAATTATGAATTCAAGAACATGTGAGGATCAACCCATTGAACTATGTTATGAACTATGGTTGAAAATTTTAAAAATGAAAATAGATATTGTAAAAGTTGTAGAGATGATGGGCTCGGTTATCTATGAAGGAATCTGAAATGTTAGAACAAAGTTGACACACGTTGATAATAATAAGAGGATATTTGGTGAGTGGTAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGGTTATTTTTTCTCCTTTGCTTGACTTTTACTTTGAAGTTCGTGTCTGTTTGTTTGTATGCCTTACCTCTTTCAACCTCTGCAGTTTGTGAGTGAACTGGTTAGAGTTGCAGCCCCAGGAGCCACCATAATTATAGTGACATGGTGCCATAGAGACCTTGGGCCTTCTGAGGACTCCTTGCAGCCATGGGAGCACAAACTCCTTCAGAAGATCTGTGATGGCTTCTATCTCCCTGCTTGGTGCTCCACTGCTGATTACGTCAAATTACTCGAGTCCCACGGTCTTCAGGTATCATACTCCTCCTACTTTGAGCATATCATATCTATTCATTCGTCGTCTTTGAAATTTAAGCCTCGTGGATTGACATATGAGATCCTACATTGGTTGGAGGAAGAAACGAAGCATTCTTTATAAGGGTGTGGACACCTCTCCCTTGTAGCAGACATTAGCTACTAGCTTTGGGCTTGACTGTTACAAATTGTATAGAGCTTAACCTCGGGTAGTATGCCAACGAGGACGCTGGCCCCCAAGAGAGGTGAATTGTGATATCCCACATTAATTAGAGAAAGGAACGAAACATTCCTTTATAAGAATATAGAAACCTCTTCTGAATAGGCACGTTTTAAAACGGTAAAGCTAACGGCGATACATAACGACCCTAGTTGTAGTGGGTTTAACTATTGTAGACATAGTTAATGCCAATAAAAGGACTCAACCGAATAAGCTCGATCTTTCATTACACGAGTATTGATATTCACGTGTACGAAGGAATCATCTAGAATGGAGGGTAGAAGTTAATGAATTGATGAAACCCATGTGGGATTTTGAGCTAAAAACGTGATGTGAAAAGGCATTGTTGTGGTGTTGAACAGGATGTAAGGACGGCAGACTGGTCTAGGAATGTTGCTCCGTTTTGGCCAGCAGTTATTCGGTCAGCATTCACTTGGAATGGCTTCTCATCGCTCTTGCGAAGTGGTAAATTAAATAAGTAAATTATATATTTTTTTTACCACTTAAAAAAGGTATTCACAATTGTTACTTTGTTGGGTGAAAAACAGGGTGGAAAACAATAAAAGGGGCATTAGTAATGCCATTGATGATTGAAGGATTCAAGAAAGATCTCATCAAATTTGCCATTATTACTTGCCGCAAGCCTGAATGAAGCTTCCTCTACACCTAATACCAAATACAATTTACCCCCCCCCAAAAAAATCCATCTTATTAGTTCATTTCTAGTTTTTTTTTCTTTCTTGTGTGTTTATTGTAAGTTAGTTTTTTTTAATCTGAAAATAGGTTCAAATTTCTCTATGGTAGGAATAATAGTCCAAGTTATTTTTATGCGTTAGTCAAACTTCAACTTGTCCTTTTCCTTTCTAATATCGATTGTTGGAGACCTTAGACCAACCCGAACATGAGGTTTAGGAGTTTATACGGGATTGGAGGAATCTTTTAGACCAACTTAAAATTTTCAGTTGGTTGGATTGGTAATCTGAGCAACTCGAACATATTTTACAATCCAACCTAACTTTCTATTTTCAAGTTGGGTTGTGTTTGGGTCGTGTAGGGTCGTTTTTTTTCATTTTTTTTACTTATTTGAAAATCATATTTATCTTTTAT

mRNA sequence

AAAAAGAAAATCGCGTAGTTTTTTCTGTATCTTCTCTCACAGAATCTCTGCGCGGCTTTACTTTCCCAACGACTTGTCGTCGGCCATTGCTCCTTTCTTTATAAATTGTAAGCAGCAATGAGCTTCACCACTGCAACTCCAATCCTTACGCCTTCCTATTCCCTCTCCTCCCTCCATTCCAATCGCTTCAACGCCACAATCCTTTCTCCGATTCCTCTCCGTCGTTCCTTTTCGTCTTTCGCTCGTCGCCGCCGCAACTGCCCTCTGATTGCCGCTGCCGCGACGACGACGGACGTTGAGATCGATATGGATGTCGATAGCCTCAAGAAGGGAATCGCTGAGTTCTATGATGAATCGTCGGGGCTTTGGGAGAACGTTTGGGGTGAGCATATGCACCATGGTTTTTACGATCCTGATTCTTCCGTTTCTCTCTCTGATCACCGTGCCGCGCAAATCCGGATGATTGAGGAGACGCTCCGGTTTGCCGGTGTGGAGGCGGCGACGGCGGTGGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCGAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCCAAACGCGCTCAAGCAATTGCTGCTGATGCTGGATTAAGTGATAAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTGAGTGAACTGGTTAGAGTTGCAGCCCCAGGAGCCACCATAATTATAGTGACATGGTGCCATAGAGACCTTGGGCCTTCTGAGGACTCCTTGCAGCCATGGGAGCACAAACTCCTTCAGAAGATCTGTGATGGCTTCTATCTCCCTGCTTGGTGCTCCACTGCTGATTACGTCAAATTACTCGAGTCCCACGGTCTTCAGGATGTAAGGACGGCAGACTGGTCTAGGAATGTTGCTCCGTTTTGGCCAGCAGTTATTCGGTCAGCATTCACTTGGAATGGCTTCTCATCGCTCTTGCGAAGTGGGTGGAAAACAATAAAAGGGGCATTAGTAATGCCATTGATGATTGAAGGATTCAAGAAAGATCTCATCAAATTTGCCATTATTACTTGCCGCAAGCCTGAATGAAGCTTCCTCTACACCTAATACCAAATACAATTTACCCCCCCCCAAAAAAATCCATCTTATTAGTTCATTTCTAGTTTTTTTTTCTTTCTTGTGTGTTTATTGTAAGTTAGTTTTTTTTAATCTGAAAATAGGTTCAAATTTCTCTATGGTAGGAATAATAGTCCAAGTTATTTTTATGCGTTAGTCAAACTTCAACTTGTCCTTTTCCTTTCTAATATCGATTGTTGGAGACCTTAGACCAACCCGAACATGAGGTTTAGGAGTTTATACGGGATTGGAGGAATCTTTTAGACCAACTTAAAATTTTCAGTTGGTTGGATTGGTAATCTGAGCAACTCGAACATATTTTACAATCCAACCTAACTTTCTATTTTCAAGTTGGGTTGTGTTTGGGTCGTGTAGGGTCGTTTTTTTTCATTTTTTTTACTTATTTGAAAATCATATTTATCTTTTAT

Coding sequence (CDS)

ATGAGCTTCACCACTGCAACTCCAATCCTTACGCCTTCCTATTCCCTCTCCTCCCTCCATTCCAATCGCTTCAACGCCACAATCCTTTCTCCGATTCCTCTCCGTCGTTCCTTTTCGTCTTTCGCTCGTCGCCGCCGCAACTGCCCTCTGATTGCCGCTGCCGCGACGACGACGGACGTTGAGATCGATATGGATGTCGATAGCCTCAAGAAGGGAATCGCTGAGTTCTATGATGAATCGTCGGGGCTTTGGGAGAACGTTTGGGGTGAGCATATGCACCATGGTTTTTACGATCCTGATTCTTCCGTTTCTCTCTCTGATCACCGTGCCGCGCAAATCCGGATGATTGAGGAGACGCTCCGGTTTGCCGGTGTGGAGGCGGCGACGGCGGTGGTGGATGTTGGGTGTGGGATTGGGGGAAGTTCGAGGTATTTGGCGAGGAAGTTTGGGGCGAAATGTAGAGGTATAACTTTGAGCCCTGTTCAAGCCAAACGCGCTCAAGCAATTGCTGCTGATGCTGGATTAAGTGATAAGGTGTGTTTTGAAGTGGCAGATGCTCTGAACCAACCATTCTCAGATGGGGAATTTGATCTGGTATGGTCCATGGAGAGTGGGGAGCACATGCCTGACAAATCCAAGTTTGTGAGTGAACTGGTTAGAGTTGCAGCCCCAGGAGCCACCATAATTATAGTGACATGGTGCCATAGAGACCTTGGGCCTTCTGAGGACTCCTTGCAGCCATGGGAGCACAAACTCCTTCAGAAGATCTGTGATGGCTTCTATCTCCCTGCTTGGTGCTCCACTGCTGATTACGTCAAATTACTCGAGTCCCACGGTCTTCAGGATGTAAGGACGGCAGACTGGTCTAGGAATGTTGCTCCGTTTTGGCCAGCAGTTATTCGGTCAGCATTCACTTGGAATGGCTTCTCATCGCTCTTGCGAAGTGGGTGGAAAACAATAAAAGGGGCATTAGTAATGCCATTGATGATTGAAGGATTCAAGAAAGATCTCATCAAATTTGCCATTATTACTTGCCGCAAGCCTGAATGA

Protein sequence

MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDVEIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVIRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Homology
[Close]
BLAST of Carg25475 vs. NCBI nr
Match: KAG7011011.1 (Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 712.6 bits (1838), Expect = 1.7e-201
Identity = 349/349 (100.00%), Postives = 349/349 (100.00%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
           EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]
BLAST of Carg25475 vs. NCBI nr
Match: KAG6571208.1 (Tocopherol O-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 710.7 bits (1833), Expect = 6.3e-201
Identity = 348/349 (99.71%), Postives = 348/349 (99.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           MSFTTATPILTPSYSLSSLH NRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV
Sbjct: 1   MSFTTATPILTPSYSLSSLHCNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
           EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]
BLAST of Carg25475 vs. NCBI nr
Match: XP_022944690.1 (tocopherol O-methyltransferase, chloroplastic [Cucurbita moschata])

HSP 1 Score: 710.7 bits (1833), Expect = 6.3e-201
Identity = 348/349 (99.71%), Postives = 348/349 (99.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           MSFTTATPILTPSYSLSSLHSNRFNA ILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNAAILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
           EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]
BLAST of Carg25475 vs. NCBI nr
Match: XP_023513404.1 (tocopherol O-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 710.3 bits (1832), Expect = 8.2e-201
Identity = 348/349 (99.71%), Postives = 348/349 (99.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSF RRRRNCPLIAAAATTTDV
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFFRRRRNCPLIAAAATTTDV 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
           EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]
BLAST of Carg25475 vs. NCBI nr
Match: XP_022986559.1 (tocopherol O-methyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 689.9 bits (1779), Expect = 1.1e-194
Identity = 340/350 (97.14%), Postives = 342/350 (97.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSF-SSFARRRRNCPLIAAAATTTD 60
           MSFT ATPILTPSYSLSSLHSNRF ATILSPIPLRRSF SSF R RRNC LIA AATTTD
Sbjct: 1   MSFTAATPILTPSYSLSSLHSNRFKATILSPIPLRRSFSSSFTRCRRNCSLIATAATTTD 60

Query: 61  VEIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120
           VEIDMDVD LK+GIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET
Sbjct: 61  VEIDMDVDRLKEGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180
           LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
           CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300
           PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKL+ESHGLQDVRTADWSRNVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLVESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350

[Close]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match: Q9ZSK1 (Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=VTE4 PE=1 SV=2)

HSP 1 Score: 447.2 bits (1149), Expect = 1.7e-124
Identity = 229/356 (64.33%), Postives = 264/356 (74.16%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           M  T A P    S    +  S    +++L   P   S  S    R N   +AAAAT+T  
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTRGNV-AVAAAATST-- 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSD--HRAAQIRMIEE 120
                 ++L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSSV LSD  H+ AQIRMIEE
Sbjct: 61  ------EALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEE 120

Query: 121 TLRFAGV------EAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAD 180
           +LRFAGV      +    VVDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +AA 
Sbjct: 121 SLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAA 180

Query: 181 AGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVT 240
             L+ K  F+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  IIIVT
Sbjct: 181 QSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVT 240

Query: 241 WCHRDLGPSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNV 300
           WCHR+L   E++LQPWE  +L KIC  FYLPAWCST DYV LL+SH LQD++ ADWS NV
Sbjct: 241 WCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENV 300

Query: 301 APFWPAVIRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 349
           APFWPAVIR+A TW G  SLLRSG K+IKGAL MPLMIEG+KK +IKF IITC+KP
Sbjct: 301 APFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

[Close]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match: Q6ZIK0 (Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=VTE4 PE=2 SV=1)

HSP 1 Score: 445.3 bits (1144), Expect = 6.5e-124
Identity = 209/286 (73.08%), Postives = 241/286 (84.27%), Query Frame = 0 

Query: 69  LKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGV--- 128
           LK+GIA  YDESSG+WE++WGEHMHHGFYD   + S+SDHR AQIRMIEE+L FA V   
Sbjct: 74  LKEGIAGLYDESSGVWESIWGEHMHHGFYDAGEAASMSDHRRAQIRMIEESLAFAAVPDD 133

Query: 129 --EAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEV 188
             +   +VVDVGCGIGGSSRYLA K+GA+C GITLSPVQA+R  A+AA+ GLSDKV F+V
Sbjct: 134 AEKKPKSVVDVGCGIGGSSRYLANKYGAQCYGITLSPVQAERGNALAAEQGLSDKVSFQV 193

Query: 189 ADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSED 248
            DAL QPF DG+FDLVWSMESGEHMPDK +FVSEL RVAAPGA IIIVTWCHR+L PSE+
Sbjct: 194 GDALEQPFPDGQFDLVWSMESGEHMPDKRQFVSELARVAAPGARIIIVTWCHRNLEPSEE 253

Query: 249 SLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVIRSA 308
           SL+P E  LL++ICD +YLP WCS +DYVK+ ES  L+D+RTADWS NVAPFWPAVI+SA
Sbjct: 254 SLKPDELNLLKRICDAYYLPDWCSPSDYVKIAESLSLEDIRTADWSENVAPFWPAVIKSA 313

Query: 309 FTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
            TW G +SLLRSGWKTI+GA+VMPLMIEG+KK LIKF IITCRKPE
Sbjct: 314 LTWKGLTSLLRSGWKTIRGAMVMPLMIEGYKKGLIKFTIITCRKPE 359

[Close]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match: W5U2K2 (3-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus roseus OX=4058 GN=NMT PE=1 SV=1)

HSP 1 Score: 259.2 bits (661), Expect = 6.6e-68
Identity = 124/288 (43.06%), Postives = 196/288 (68.06%), Query Frame = 0 

Query: 66  VDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGV 125
           ++  ++ +AEFYD+ +G W+  +G H+H G+Y+P ++ +++  + A IRMI+E LRFAGV
Sbjct: 1   MEEKQEKVAEFYDKVTGAWDLFYGVHLHDGYYEPGTTATMAISQDAVIRMIDELLRFAGV 60

Query: 126 EAATA-----VVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 185
               A     ++DVG G+GG+  Y+A+K+  +C GIT+SP Q K AQ  AA  G+ +KV 
Sbjct: 61  SEDPAKKPRSMLDVGSGLGGTCVYVAKKYDIQCTGITISPNQVKYAQDYAATEGVENKVS 120

Query: 186 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 245
           F+  DAL+ P+SDG+FD+V+++   EH+ DK KF+ E+VRVAAPGA III +  H +L P
Sbjct: 121 FDCGDALDMPYSDGKFDVVFTINCIEHVHDKEKFIREMVRVAAPGAAIIIASQAHPNLSP 180

Query: 246 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 305
            E SL+P + K+LQKICDG    + CS+ DYV+ L    +++++ ADW++N+ P +P ++
Sbjct: 181 GE-SLKPRDKKILQKICDGAGAVSLCSSDDYVRWLTPLPVKEIKAADWTQNITPLYPLLM 240

Query: 306 RSAFTWNGFSSL-LRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRK 348
           + AFTW GF+S+ L+ GW+ I     + L+ +     ++KFA++T RK
Sbjct: 241 KEAFTWKGFTSIVLKGGWRAINLINAVRLVAKAANDGILKFAVVTGRK 287

[Close]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match: P74388 (2-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=sll0418 PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.7e-36
Identity = 93/253 (36.76%), Postives = 135/253 (53.36%), Query Frame = 0 

Query: 73  IAEFYDE--SSGLWENVWGEHMHHGFY-DPDSSVSLSDHRAAQIRMIEETLRFAGVEA-- 132
           +A  YD+    G+ E  WG+H+H G Y DP  +    D   ++I  +    ++ G++   
Sbjct: 36  VANAYDQWTEDGILEYYWGDHIHLGHYGDPPVA---KDFIQSKIDFVHAMAQWGGLDTLP 95

Query: 133 -ATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEVADA 192
             T V+DVGCGIGGSSR LA+ +G    GIT+SP Q KRA  +      +    F V DA
Sbjct: 96  PGTTVLDVGCGIGGSSRILAKDYGFNVTGITISPQQVKRATELTPPDVTAK---FAVDDA 155

Query: 193 LNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQ 252
           +   F DG FD+VWS+E+G HMPDK+ F  EL+RV  PG  +++  W  RD    +  L 
Sbjct: 156 MALSFPDGSFDVVWSVEAGPHMPDKAVFAKELLRVVKPGGILVVADWNQRD--DRQVPLN 215

Query: 253 PWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQD--VRTADWSRNVAPFWPAVIRSAF 312
            WE  +++++ D +  PA+ S   + + LE+ GL +  V TADW+    P W   I    
Sbjct: 216 FWEKPVMRQLLDQWSHPAFASIEGFAENLEATGLVEGQVTTADWTVPTLPAWLDTI---- 273

Query: 313 TWNGFSSLLRSGW 318
            W G   +   GW
Sbjct: 276 -WQGI--IRPQGW 273

[Close]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match: Q8KZ94 (Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX=68170 GN=rebM PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 6.9e-25
Identity = 60/159 (37.74%), Postives = 94/159 (59.12%), Query Frame = 0 

Query: 73  IAEFYDESSGLWENVWGEHMHHGFY-DPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAV 132
           + + YD+ +  +  +WGE++H G++ D  + VS+ D   A  R+ +E +    V +   V
Sbjct: 19  VRQMYDDFTDPFARIWGENLHFGYWEDAGADVSVDD---ATDRLTDEMIALLDVRSGDRV 78

Query: 133 VDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEVADALNQPF 192
           +DVGCGIG  +  LA     +  GI++S  Q  +A A A  AGL+++V F  ADA++ PF
Sbjct: 79  LDVGCGIGKPAVRLATARDVRVTGISISRPQVNQANARATAAGLANRVTFSYADAMDLPF 138

Query: 193 SDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIII 231
            D  FD VW++ES  HMPD+ + + E+ RV  PG T+ I
Sbjct: 139 EDASFDAVWALESLHHMPDRGRALREMARVLRPGGTVAI 174

[Close]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match: A0A6J1FYT6 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449074 PE=3 SV=1)

HSP 1 Score: 710.7 bits (1833), Expect = 3.0e-201
Identity = 348/349 (99.71%), Postives = 348/349 (99.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           MSFTTATPILTPSYSLSSLHSNRFNA ILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV
Sbjct: 1   MSFTTATPILTPSYSLSSLHSNRFNAAILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
           EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL
Sbjct: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI
Sbjct: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 349

[Close]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match: A0A6J1JGD5 (tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484262 PE=3 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 5.6e-195
Identity = 340/350 (97.14%), Postives = 342/350 (97.71%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSF-SSFARRRRNCPLIAAAATTTD 60
           MSFT ATPILTPSYSLSSLHSNRF ATILSPIPLRRSF SSF R RRNC LIA AATTTD
Sbjct: 1   MSFTAATPILTPSYSLSSLHSNRFKATILSPIPLRRSFSSSFTRCRRNCSLIATAATTTD 60

Query: 61  VEIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120
           VEIDMDVD LK+GIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET
Sbjct: 61  VEIDMDVDRLKEGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEET 120

Query: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180
           LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV
Sbjct: 121 LRFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKV 180

Query: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240
           CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG
Sbjct: 181 CFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLG 240

Query: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAV 300
           PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKL+ESHGLQDVRTADWSRNVAPFWPAV
Sbjct: 241 PSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLVESHGLQDVRTADWSRNVAPFWPAV 300

Query: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE
Sbjct: 301 IRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350

[Close]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match: A0A5D3BSC2 (Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold602G001840 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 299/349 (85.67%), Postives = 312/349 (89.40%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           M+FT ATPI T  Y  SSLH NR N TIL+PI LR SFSSF   RR+ P +A AATT   
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFT-PRRSSPFLAFAATTASD 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
              MD   L+KGIAEFYD+SSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQ+RMIEETL
Sbjct: 61  H--MDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVE ATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTW GF+SLLR+GWKTIKGALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 346

[Close]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match: A0A1S3CHR0 (tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103501078 PE=3 SV=1)

HSP 1 Score: 599.0 bits (1543), Expect = 1.3e-167
Identity = 299/349 (85.67%), Postives = 312/349 (89.40%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           M+FT ATPI T  Y  SSLH NR N TIL+PI LR SFSSF   RR+ P +A AATT   
Sbjct: 1   MNFTAATPIHTSFYPPSSLHFNRLNPTILNPISLRPSFSSFT-PRRSSPFLAFAATTASD 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
              MD   L+KGIAEFYD+SSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQ+RMIEETL
Sbjct: 61  H--MDSHRLQKGIAEFYDQSSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQVRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVE ATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAA  GLSDKV 
Sbjct: 121 RFAGVEEATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAAEGLSDKVY 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSGNVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTW GF+SLLR+GWKTIKGALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIKGALVMPLMIEGFNKDLIKFAIITCRKPQ 346

[Close]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match: A0A0A0LTM1 (Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G432780 PE=3 SV=1)

HSP 1 Score: 582.8 bits (1501), Expect = 9.6e-163
Identity = 289/349 (82.81%), Postives = 307/349 (87.97%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           M+ T ATPI T SY  SSLHSN FN+  +SP P   SFSSF  RR +  L +++A TT  
Sbjct: 1   MNLTAATPIHTSSYPPSSLHSNPFNS--ISPRP---SFSSFTPRRFSPLLASSSAATTPP 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETL 120
            + MD   L KGIAEFYD+SSGLWENVWG+HMHHGFYDP SSVSLSDHRAAQIRMIEETL
Sbjct: 61  SLHMDSHRLHKGIAEFYDQSSGLWENVWGQHMHHGFYDPHSSVSLSDHRAAQIRMIEETL 120

Query: 121 RFAGVEAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC 180
           RFAGVEAATAVVDVGCGIGGSSRYLA KFGAKCRGITLSPVQAKRAQ I+A  GLSDKVC
Sbjct: 121 RFAGVEAATAVVDVGCGIGGSSRYLATKFGAKCRGITLSPVQAKRAQEISAAEGLSDKVC 180

Query: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGP 240
           FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRV APG TIIIVTWCHRDLGP
Sbjct: 181 FEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVTAPGGTIIIVTWCHRDLGP 240

Query: 241 SEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVAPFWPAVI 300
           SE SLQPWE KLLQKICDGFYLPAWCSTADYVKLL+SH LQD++ ADWS NVAPFWPAVI
Sbjct: 241 SESSLQPWELKLLQKICDGFYLPAWCSTADYVKLLQSHNLQDIKRADWSENVAPFWPAVI 300

Query: 301 RSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKPE 350
           RSAFTW GF+SLLR+GWKTI+GALVMPLMIEGF KDLIKFAIITCRKP+
Sbjct: 301 RSAFTWKGFTSLLRTGWKTIRGALVMPLMIEGFNKDLIKFAIITCRKPQ 344

[Close]
BLAST of Carg25475 vs. TAIR 10
Match: AT1G64970.1 (gamma-tocopherol methyltransferase )

HSP 1 Score: 447.2 bits (1149), Expect = 1.2e-125
Identity = 229/356 (64.33%), Postives = 264/356 (74.16%), Query Frame = 0 

Query: 1   MSFTTATPILTPSYSLSSLHSNRFNATILSPIPLRRSFSSFARRRRNCPLIAAAATTTDV 60
           M  T A P    S    +  S    +++L   P   S  S    R N   +AAAAT+T  
Sbjct: 1   MKATLAAPSSLTSLPYRTNSSFGSKSSLLFRSPSSSSSVSMTTTRGNV-AVAAAATST-- 60

Query: 61  EIDMDVDSLKKGIAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSD--HRAAQIRMIEE 120
                 ++L+KGIAEFY+E+SGLWE +WG+HMHHGFYDPDSSV LSD  H+ AQIRMIEE
Sbjct: 61  ------EALRKGIAEFYNETSGLWEEIWGDHMHHGFYDPDSSVQLSDSGHKEAQIRMIEE 120

Query: 121 TLRFAGV------EAATAVVDVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAAD 180
           +LRFAGV      +    VVDVGCGIGGSSRYLA KFGA+C GITLSPVQAKRA  +AA 
Sbjct: 121 SLRFAGVTDEEEEKKIKKVVDVGCGIGGSSRYLASKFGAECIGITLSPVQAKRANDLAAA 180

Query: 181 AGLSDKVCFEVADALNQPFSDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVT 240
             L+ K  F+VADAL+QPF DG+FDLVWSMESGEHMPDK+KFV ELVRVAAPG  IIIVT
Sbjct: 181 QSLAHKASFQVADALDQPFEDGKFDLVWSMESGEHMPDKAKFVKELVRVAAPGGRIIIVT 240

Query: 241 WCHRDLGPSEDSLQPWEHKLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNV 300
           WCHR+L   E++LQPWE  +L KIC  FYLPAWCST DYV LL+SH LQD++ ADWS NV
Sbjct: 241 WCHRNLSAGEEALQPWEQNILDKICKTFYLPAWCSTDDYVNLLQSHSLQDIKCADWSENV 300

Query: 301 APFWPAVIRSAFTWNGFSSLLRSGWKTIKGALVMPLMIEGFKKDLIKFAIITCRKP 349
           APFWPAVIR+A TW G  SLLRSG K+IKGAL MPLMIEG+KK +IKF IITC+KP
Sbjct: 301 APFWPAVIRTALTWKGLVSLLRSGMKSIKGALTMPLMIEGYKKGVIKFGIITCQKP 347

[Close]
BLAST of Carg25475 vs. TAIR 10
Match: AT1G76090.1 (sterol methyltransferase 3 )

HSP 1 Score: 88.2 bits (217), Expect = 1.4e-17
Identity = 67/224 (29.91%), Postives = 106/224 (47.32%), Query Frame = 0 

Query: 76  FYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVG 135
           FY+  + ++E  WG+  H   + P      SD  A +I   E  +    V+    ++D G
Sbjct: 77  FYNLVTDIYEWGWGQSFHFSPHVPGK----SDKDATRIHE-EMAVDLIKVKPGQKILDAG 136

Query: 136 CGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEV-ADALNQPFSDG 195
           CG+GG  R +A    A+  GIT++  Q +RA+     AGL D +C  V  + L  PF + 
Sbjct: 137 CGVGGPMRAIAAHSKAQVTGITINEYQVQRAKLHNKKAGL-DSLCNVVCGNFLKMPFDEN 196

Query: 196 EFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEHK-LL 255
            FD  +S+E+  H P   +  SE+ RV  PG+  +   W   +    +D     EHK ++
Sbjct: 197 TFDGAYSIEATCHAPKLEEVYSEIFRVMKPGSLFVSYEWVTTEKYRDDDE----EHKDVI 256

Query: 256 QKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVA-PFW 297
           Q I  G  LP   S AD     +  G + V+  D ++  + P+W
Sbjct: 257 QGIERGDALPGLRSYADIAVTAKKVGFEVVKEKDLAKPPSKPWW 290

[Close]
BLAST of Carg25475 vs. TAIR 10
Match: AT1G20330.1 (sterol methyltransferase 2 )

HSP 1 Score: 87.4 bits (215), Expect = 2.4e-17
Identity = 66/224 (29.46%), Postives = 105/224 (46.88%), Query Frame = 0 

Query: 76  FYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAVVDVG 135
           FY+  + ++E  WG+  H   + P  S+    H+ A     E  +    V+    ++DVG
Sbjct: 77  FYNLVTDIYEWGWGQSFH---FSP--SIPGKSHKDATRLHEEMAVDLIQVKPGQKILDVG 136

Query: 136 CGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVCFEV-ADALNQPFSDG 195
           CG+GG  R +A    A   GIT++  Q  RA+     AGL D +C  V  + L  PF D 
Sbjct: 137 CGVGGPMRAIASHSRANVVGITINEYQVNRARLHNKKAGL-DALCEVVCGNFLQMPFDDN 196

Query: 196 EFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEH-KLL 255
            FD  +S+E+  H P   +  +E+ RV  PG+  +   W   +   +ED     EH +++
Sbjct: 197 SFDGAYSIEATCHAPKLEEVYAEIYRVLKPGSMYVSYEWVTTEKFKAEDD----EHVEVI 256

Query: 256 QKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSRNVA-PFW 297
           Q I  G  LP   +  D  +  +  G + V+  D +   A P+W
Sbjct: 257 QGIERGDALPGLRAYVDIAETAKKVGFEIVKEKDLASPPAEPWW 290

[Close]
BLAST of Carg25475 vs. TAIR 10
Match: AT5G13710.1 (sterol methyltransferase 1 )

HSP 1 Score: 86.3 bits (212), Expect = 5.4e-17
Identity = 65/240 (27.08%), Postives = 111/240 (46.25%), Query Frame = 0 

Query: 73  IAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAVV 132
           + ++YD ++  +E  WGE  H        S+  S  R      ++      G++    V+
Sbjct: 45  VNKYYDLATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQ-----LGIQPGQKVL 104

Query: 133 DVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC-FEVADALNQPF 192
           DVGCGIGG  R +AR   +   G+  +  Q  R + +   AG+ DK C F  AD +  PF
Sbjct: 105 DVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGV-DKTCNFVKADFMKMPF 164

Query: 193 SDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEH- 252
            +  FD V+++E+  H PD      E+ RV  PG       WC  D    +++    EH 
Sbjct: 165 PENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNA----EHQ 224

Query: 253 KLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSR-NVAPFWPAVIRSAFTWNGF 310
           K+  +I  G  LP    T   ++ L+  G + +   D ++ +  P++  + ++ F+ + F
Sbjct: 225 KIKGEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSF 274

[Close]
BLAST of Carg25475 vs. TAIR 10
Match: AT5G13710.2 (sterol methyltransferase 1 )

HSP 1 Score: 86.3 bits (212), Expect = 5.4e-17
Identity = 65/240 (27.08%), Postives = 111/240 (46.25%), Query Frame = 0 

Query: 73  IAEFYDESSGLWENVWGEHMHHGFYDPDSSVSLSDHRAAQIRMIEETLRFAGVEAATAVV 132
           + ++YD ++  +E  WGE  H        S+  S  R      ++      G++    V+
Sbjct: 45  VNKYYDLATSFYEYGWGESFHFAQRWKGESLRESIKRHEHFLALQ-----LGIQPGQKVL 104

Query: 133 DVGCGIGGSSRYLARKFGAKCRGITLSPVQAKRAQAIAADAGLSDKVC-FEVADALNQPF 192
           DVGCGIGG  R +AR   +   G+  +  Q  R + +   AG+ DK C F  AD +  PF
Sbjct: 105 DVGCGIGGPLREIARFSNSVVTGLNNNEYQITRGKELNRLAGV-DKTCNFVKADFMKMPF 164

Query: 193 SDGEFDLVWSMESGEHMPDKSKFVSELVRVAAPGATIIIVTWCHRDLGPSEDSLQPWEH- 252
            +  FD V+++E+  H PD      E+ RV  PG       WC  D    +++    EH 
Sbjct: 165 PENSFDAVYAIEATCHAPDAYGCYKEIYRVLKPGQCFAAYEWCMTDAFDPDNA----EHQ 224

Query: 253 KLLQKICDGFYLPAWCSTADYVKLLESHGLQDVRTADWSR-NVAPFWPAVIRSAFTWNGF 310
           K+  +I  G  LP    T   ++ L+  G + +   D ++ +  P++  + ++ F+ + F
Sbjct: 225 KIKGEIEIGDGLPDIRLTTKCLEALKQAGFEVIWEKDLAKDSPVPWYLPLDKNHFSLSSF 274

The following BLAST results are available for this feature:
BLAST of Carg25475 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7011011.11.7e-201100.00Tocopherol O-methyltransferase, chloroplastic [Cucurbita argyrosperma subsp. arg... [more]
KAG6571208.16.3e-20199.71Tocopherol O-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma s... [more]
XP_022944690.16.3e-20199.71tocopherol O-methyltransferase, chloroplastic [Cucurbita moschata][more]
XP_023513404.18.2e-20199.71tocopherol O-methyltransferase, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022986559.11.1e-19497.14tocopherol O-methyltransferase, chloroplastic [Cucurbita maxima][more]
BLAST of Carg25475 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9ZSK11.7e-12464.33Tocopherol O-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN... [more]
Q6ZIK06.5e-12473.08Probable tocopherol O-methyltransferase, chloroplastic OS=Oryza sativa subsp. ja... [more]
W5U2K26.6e-6843.063-hydroxy-16-methoxy-2,3-dihydrotabersonine N-methyltransferase OS=Catharanthus ... [more]
P743881.7e-3636.762-methyl-6-phytyl-1,4-hydroquinone methyltransferase OS=Synechocystis sp. (strai... [more]
Q8KZ946.9e-2537.74Demethylrebeccamycin-D-glucose O-methyltransferase OS=Lentzea aerocolonigenes OX... [more]
BLAST of Carg25475 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FYT63.0e-20199.71tocopherol O-methyltransferase, chloroplastic OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1JGD55.6e-19597.14tocopherol O-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5D3BSC21.3e-16785.67Tocopherol O-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
A0A1S3CHR01.3e-16785.67tocopherol O-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC1035... [more]
A0A0A0LTM19.6e-16382.81Methyltransf_11 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G43... [more]
BLAST of Carg25475 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G64970.11.2e-12564.33gamma-tocopherol methyltransferase [more]
AT1G76090.11.4e-1729.91sterol methyltransferase 3 [more]
AT1G20330.12.4e-1729.46sterol methyltransferase 2 [more]
AT5G13710.15.4e-1727.08sterol methyltransferase 1 [more]
AT5G13710.25.4e-1727.08sterol methyltransferase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.150Vaccinia Virus protein VP39coord: 78..318
e-value: 1.7E-58
score: 199.9
NoneNo IPR availablePANTHERPTHR43591:SF72CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASEcoord: 58..344
NoneNo IPR availablePANTHERPTHR43591METHYLTRANSFERASEcoord: 58..344
NoneNo IPR availableCDDcd02440AdoMet_MTasescoord: 131..233
e-value: 3.17466E-13
score: 63.2178
IPR013216Methyltransferase type 11PFAMPF08241Methyltransf_11coord: 132..230
e-value: 7.8E-18
score: 64.9
IPR025774SAM-dependent methyltransferase gTMT-typePROSITEPS51581SAM_GTMTcoord: 53..349
score: 145.128708
IPR029063S-adenosyl-L-methionine-dependent methyltransferaseSUPERFAMILY53335S-adenosyl-L-methionine-dependent methyltransferasescoord: 68..348

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25475-RACarg25475-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032259 methylation
biological_process GO:0010189 vitamin E biosynthetic process
molecular_function GO:0050342 tocopherol O-methyltransferase activity
molecular_function GO:0008168 methyltransferase activity