Carg25431 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25431
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionNAD(P)-bd_dom domain-containing protein
LocationCarg_Chr11: 4320207 .. 4325002 (+)
RNA-Seq ExpressionCarg25431
SyntenyCarg25431
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTAGCATTTATGTAAATTTCATATAGTCATCCTATTAATTTTCAGGTAATCAGTTTCTTCTTCACTCTTGCTCTTGCTGCTTGTTTTCTCAGTTCTCATCAATGGCTTCTAATCTCTCTCTCTCGTTCATCCCGGAATTTCATCTTCCACTTGGTAATTTTCACAAAACATTTCAAAGAGTTCCTCTCTTTGGCCTTCACCCGTTGTGTTCGTTTTCCGATAACCAAATCCGGAAGAGCATTTCAAACTGTAGAGGACGATCCGCAGCCATTCGTGTTATCAATCAGGAAGTTGTTCAATCTCCAAGTTCAGAGTCGATCCGCGAGTCGAAAACTGATCCTGTTGCATCTTCTAAACTCGTCCTTGTCGTCGGCGCTTCCGGAGGCGTCGGTATGTTCTATCTGAATTCTCCTCTGCATTTTTCCGTAGAATATATATGGACGTACATGATGAATTGATTAAGTTAAACTGAATCAGGTAGTGTTAGGTAGTTAGTTTAATGTTAATCTCGTCATGAATCTTTTAAAATTGAGTGTATCGGTTGTAGTTCTTAGTGAGGAAGGTTGTGGTGATTTTGGTAAAGTTTTTAGCTGTTATTCTTGCTAGTTATTGCATGGTGAGATTATGCCCTAGTAATCAACGATCTATATTGGAGGATTTGTTTTGTCCGCTAGGTGATTTATGTGCTTGGTTTGATGTTTTCACTTTCTTTTATAGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTGTTACTGCGAGATCCCGAGAAAGCAACTACATTATTTGGTGAACAGGACGAAGATATGTTACAGGTATTATGATCTCTCTTAGTTGTGCATGGTCATGGCACGGAAGATAATGGTTTCGTGTCCCATTTTGCTCGGTTGAGTATAGCATATAGAGAGTGGACTTGGGATCTAAAAGGCTATTCTGTCACTTTAATCGATTGGCTTCAATTTCTATTTCCATTGATGTGATTAGGAACAAGTTAGAATGTTTGATGCAACTTCTTATTCATTGCCCTATGTGGTCTGGAAAATAAGTTGATTTTAAAAGTTTCACCATACTCTCAAGTAGTCTTTGTTGTTACAGAGAGTTTTAAATTTGACAGGTAAGCAAAGGTGATACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGTTTGTGATCTCGATTGGATTACATTACATGACCTTACCATGTTGTATCTGTATCAAATTCCTTAACAATGCTTTAATTGTAGAAAAACTCCTTAACTCATTTGATTCTTCACGGGCACCCCTTTGGCACGGCTGTACTGCCTATAATTATCAGCTTTTAGCCACTTTCAATCTTTTCATATTGACACTAACCTTGCTTGAAATTGTACTGTTTGTTCTCTTCTCCCTAGCTTCTCTCATGAGGAATCAGACTTTTGGCATGGGATGAACTCTTGTTCCTCATGAATAGTGAAAAATGGCTCTTTCCTCCTGTCTATGGAGATACAAAACCAAGGGAAAGAGAGAAACGTACTTTTATTAAGCGAGGAGGGAAGATTGCCTCTTTACTCATGTAGAGGGAAGTATCATGTTAGGAGTGCAAACATAATCCCACATTCACTAGGAAGAGATTGATCATAGGTATATAACCGAGGACAATTATTTTCATTGGTATATGGTTTTGTGGGGAAACCAAAAGTAAAGCCACGAGTGTTTATATTCAAGGTACACAATGTCATTCCATTGTGGGGATAGGTGAAGGTTCCATGGTTCTTTATATGCAAATTTTGAAATAGATAATGACGAAAAACTAAAAGTTAAAATTTTAATAATTATTGTTATTGTTATTATAATAAGATCAAGCTAGAAGCCAAAAATTACAATATAAATTAGATTGAAATAATTGTGAACCTGAAGTTAAAATATTAATGATTATGGTTATTGTTATTATAATAAGAACAAGCTAGAAACCAAAAATTACAATATAAATTAGATTGAAATAAATATGAACCTAAATAATAAACCATACTATTGGCATGTTGTTCTCGCATTCTCAAGAAGGCTAGCGTTGGGCTATGATCTTGGACTTAACAGTTGGATCCCTCGTCTATTATTACCGTAGACTTTACTTTTTTTTTTTTTTATCGCACTTTTGTTACAGATTTATATGTATATATATTTTTTCAGATTCCTTTTTGAATTATATTGGAATAGATTAAGTAGTCCCCGTTTTGGATTGTAATTGTTGATTTTAGTTTTTTTCTCTCTCTCTCATAGAACTCTATGATAGATATGATTTTTATTGTTCTTTCTTATTTTAAAATATTATAACTGAACACTTTTGGTAGTATCAATTGTTTATAAATTTAATATGTCTTTTTTTATTTGTCTGATAATCATCTATTAGGGTGTCACGCATGTGATTTGCTGCACAGGAACAACAGCCTTCCCTTCAAAGCGATGGGATGGGGATAACACTCCTGAACGAGTAGGTTAGGATTATACTGTTGATATCAGATGCTACATTTTGGCGTTTTGATGCTACATTTTGGCGTTTTGGCTAGAAACTTAGACCTATAGATCTTTGTTGAAATCAATATAAACATCGGACAATGTTTTTGTTTCCATTCTCTAGATTTATAGTTGCATATCTTCGAATACTCGTAAGCACACTAACACACCTATTATTATACCTATGCTTCTCGGCACTTTCTGACTATGAACTTATGACTGTAGATTGGGAGGGTGTGAGAAACCTTATATCAGCACTTCCTCGATCAGTAAAAAGAGTAGTTCTTGTTTCATCAATAGGTGTAACCAAATTCAATGAATTGCCATGGAGGTAGGTCACTCTTTTCACTGTCGAGAATAATTCTTCTAGGAAATAATAAAGATTGGTTTATTAGAGTCCATGCTAAAAGTGTTTCTTCTAAAATGCATAACCTGCATCACTAAAATTTGTCATCTCTTATAACCATAGACTCTATGGATGTGTTCTCTAGTTCGTCAAACTGATCTTTGTTTCTTCAGAATGTTCAACTATATTGACATCTGCAGACAAGTATCAATTTTATGTTGCGTATCTGTATCACCTCATTGCTCCTAATACTAAACAGTAAATACATACTGCGTCTAAATACCAAAAGGATAATCAGACTGATAATCATGCAGCATAAACTTCGATGGTTTATTATTTATATTTCTCAGCAATCAGCACGAGGCTTGAATACACACATTTACCTTTTATTGTTATCGTGTTTACAACTTGGTATAGGAAGCAGACGAGTCTGTGACTGCATTTGTTTCTTTGACTCCTTTACAAACTATGCTTTCAATCCACTAATTGGTTTCATTTTTTCCTAGCATTATGAACCTTTTTGGTGTTCTCAAATACAAAAGGCAAGGGGAGGATTTTCTTCGTGACTCAGGTCTTCCCTTTACAATAATCAGGTATGTGTAGTTGGCATGAGCTTCTTATTTTACCTGAGCTAACCTTTTTTCTTTTTTTTGTTTACTCTAGACATGTAACTCTGTTTTTCAACATTGAGCATCCACTCTCTCTTTTATGTACAAAATTGTGTGTTCCAGAGCTGGTAGGTTGACTGATGGACCATACACCTCATATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAACGGCGTGCTGTCCTCCTTGGTCAAGGTACGACTTTATAAAATTTGTAAAAATGACAAATGTTGCATTGCCACTATAGAATGTGGTGTATGAGAACCCGTTAGTTTCGTTATCCTTAATCTTTTTTACCAGTGATTAATCCTATGGCCTTGTTTCTATACTTCTCCATAATTCTAATCACTTACAATGAAAATAAGATAACCTGATTGATTACTATTTGGTTTTTTATTTTTGAAAACTAAACTTATGGTTATCTATTTTCTAACTTCAAAATATCAGCCAAGTTAGGAAAACTAAAAAAGAAATGCAGTTTTTAAAAACATGCAATCTATTTTTGGAATATGATAAAGAATTTAAATGTTTCTTTGAGAAAGGTGAAAATTGTAGTAAAGAAATTGGTATAAAACAAGCATAAATTTCAAGGACAAAATTGTTTATAAAACGGGTCCAAAATTTAAAACAAAAAAAAAAAAAGTTTTAAAGTGAGTAGCCAGGTATGAAAATTTACAATCGTATACACATGTTAATACAATTTTCAATTAGTATCTTTTTCCATTTTTGTCATTATATTCTCGTTCCTTTCTTGTTAGTTAACATATTCGAGTTCACATTGAAATTATGTCGTTCTTTTTCCCTCTCGTGGGTCTCAGGTGACAAACTTGTTGGAGAGGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTGGACATTGATTTCACTGAAGGACAGGCGTACGAAATTAACTCAGTTGAGGTGAGCTTCCAAGATAGCTGCAGTTTATGAGAATAAAAGCTTCTTTGAGAACTGGTTTATAAACAAATTTAAAACTTGGTTGAATAATTATTTCTAAAAAGCCATTTCTCTTGCTTCTTCCCCTTTATTGTATTTTCATAAATAATTTTTAGAACAAATAAGACTGTTCGTTTGTATTGCTTTACAAATCATCTGATTATTGTTGTTAAAAATAGTTTTGAAAACAAAAAATAGAAAGCTACAACTAGTCGATTCTTGTTAGATCTCTCTATCTCTCTTTTGAAAAAGTAATAAAACTTCGAAACTTTTGCAGGGGGAGGGTCCAGGGAATGATCCTCAGAAGTGGAGTGAACTATTCAGAGCTGCAACACAGTGA

mRNA sequence

TTAGCATTTATGTAAATTTCATATAGTCATCCTATTAATTTTCAGGTAATCAGTTTCTTCTTCACTCTTGCTCTTGCTGCTTGTTTTCTCAGTTCTCATCAATGGCTTCTAATCTCTCTCTCTCGTTCATCCCGGAATTTCATCTTCCACTTGGTAATTTTCACAAAACATTTCAAAGAGTTCCTCTCTTTGGCCTTCACCCGTTGTGTTCGTTTTCCGATAACCAAATCCGGAAGAGCATTTCAAACTGTAGAGGACGATCCGCAGCCATTCGTGTTATCAATCAGGAAGTTGTTCAATCTCCAAGTTCAGAGTCGATCCGCGAGTCGAAAACTGATCCTGTTGCATCTTCTAAACTCGTCCTTGTCGTCGGCGCTTCCGGAGGCGTCGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTGTTACTGCGAGATCCCGAGAAAGCAACTACATTATTTGGTGAACAGGACGAAGATATGTTACAGGTAAGCAAAGGTGATACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGGTGTCACGCATGTGATTTGCTGCACAGGAACAACAGCCTTCCCTTCAAAGCGATGGGATGGGGATAACACTCCTGAACGAGTAGATTGGGAGGGTGTGAGAAACCTTATATCAGCACTTCCTCGATCAGTAAAAAGAGTAGTTCTTGTTTCATCAATAGGTGTAACCAAATTCAATGAATTGCCATGGAGCATTATGAACCTTTTTGGTGTTCTCAAATACAAAAGGCAAGGGGAGGATTTTCTTCGTGACTCAGGTCTTCCCTTTACAATAATCAGACATGTAACTCTGTTTTTCAACATTGAGCATCCACTCTCTCTTTTATGTACAAAATTAGCTGGTAGGTTGACTGATGGACCATACACCTCATATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAACGGCGTGCTGTCCTCCTTGGTCAAGGTGACAAACTTGTTGGAGAGGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTGGACATTGATTTCACTGAAGGACAGGCGTACGAAATTAACTCAGTTGAGGGGGAGGGTCCAGGGAATGATCCTCAGAAGTGGAGTGAACTATTCAGAGCTGCAACACAGTGA

Coding sequence (CDS)

ATGGCTTCTAATCTCTCTCTCTCGTTCATCCCGGAATTTCATCTTCCACTTGGTAATTTTCACAAAACATTTCAAAGAGTTCCTCTCTTTGGCCTTCACCCGTTGTGTTCGTTTTCCGATAACCAAATCCGGAAGAGCATTTCAAACTGTAGAGGACGATCCGCAGCCATTCGTGTTATCAATCAGGAAGTTGTTCAATCTCCAAGTTCAGAGTCGATCCGCGAGTCGAAAACTGATCCTGTTGCATCTTCTAAACTCGTCCTTGTCGTCGGCGCTTCCGGAGGCGTCGGACAGTTGGTGGCAGCAGCTTTGCTTGACCGGAATGTCAAATTACGCCTGTTACTGCGAGATCCCGAGAAAGCAACTACATTATTTGGTGAACAGGACGAAGATATGTTACAGGTAAGCAAAGGTGATACAAGGAACCCAGAAGACCTAGATCCATCTATATTTGAGGGTGTCACGCATGTGATTTGCTGCACAGGAACAACAGCCTTCCCTTCAAAGCGATGGGATGGGGATAACACTCCTGAACGAGTAGATTGGGAGGGTGTGAGAAACCTTATATCAGCACTTCCTCGATCAGTAAAAAGAGTAGTTCTTGTTTCATCAATAGGTGTAACCAAATTCAATGAATTGCCATGGAGCATTATGAACCTTTTTGGTGTTCTCAAATACAAAAGGCAAGGGGAGGATTTTCTTCGTGACTCAGGTCTTCCCTTTACAATAATCAGACATGTAACTCTGTTTTTCAACATTGAGCATCCACTCTCTCTTTTATGTACAAAATTAGCTGGTAGGTTGACTGATGGACCATACACCTCATATGATCTTAATACTTTGCTCAAAGCTACAGCAGGGCAACGGCGTGCTGTCCTCCTTGGTCAAGGTGACAAACTTGTTGGAGAGGCCAGCAGGATTGTGGTTGCTGAAGCTTGCATACAGGCTCTGGACATTGATTTCACTGAAGGACAGGCGTACGAAATTAACTCAGTTGAGGGGGAGGGTCCAGGGAATGATCCTCAGAAGTGGAGTGAACTATTCAGAGCTGCAACACAGTGA

Protein sequence

MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVINQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEKATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERVDWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLPFTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Homology
BLAST of Carg25431 vs. NCBI nr
Match: KAG7022102.1 (putative protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 706.1 bits (1821), Expect = 1.6e-199
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI
Sbjct: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 353

BLAST of Carg25431 vs. NCBI nr
Match: KAG6588205.1 (putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 655.2 bits (1689), Expect = 3.2e-184
Identity = 334/353 (94.62%), Postives = 334/353 (94.62%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI
Sbjct: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. NCBI nr
Match: XP_022928583.1 (uncharacterized protein At2g37660, chloroplastic [Cucurbita moschata])

HSP 1 Score: 653.3 bits (1684), Expect = 1.2e-183
Identity = 333/353 (94.33%), Postives = 333/353 (94.33%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI
Sbjct: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVRKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. NCBI nr
Match: XP_023530592.1 (uncharacterized protein At2g37660, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 645.6 bits (1664), Expect = 2.5e-181
Identity = 329/353 (93.20%), Postives = 332/353 (94.05%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFHLPLGNFHKTFQRV LFGLHPLCSFSDNQIRKSIS+CRGRSAAIRVI
Sbjct: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVSLFGLHPLCSFSDNQIRKSISSCRGRSAAIRVI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQV KGDTRNP+DLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVRKGDTRNPDDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGE+FLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEEFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. NCBI nr
Match: XP_023004425.1 (uncharacterized protein At2g37660, chloroplastic [Cucurbita maxima])

HSP 1 Score: 642.5 bits (1656), Expect = 2.1e-180
Identity = 328/353 (92.92%), Postives = 330/353 (93.48%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFH PLGNFHKTFQRVPLFGLHPLCSFS+ QIRKSIS+CRGRSAAIR I
Sbjct: 1   MASNLSLSFIPEFHRPLGNFHKTFQRVPLFGLHPLCSFSNYQIRKSISSCRGRSAAIRAI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVRKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. ExPASy Swiss-Prot
Match: O80934 (Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g37660 PE=1 SV=2)

HSP 1 Score: 96.3 bits (238), Expect = 7.5e-19
Identity = 87/285 (30.53%), Postives = 133/285 (46.67%), Query Frame = 0

Query: 87  VLVVGASGGVGQLVAAALLDRNVKL--RLLLRDPEKATTLFGEQDEDMLQVSKGDTRNPE 146
           VLV GA G  GQ+V   L +R+ +   R L+R  E    + GE      +V  GD R+  
Sbjct: 79  VLVTGAGGRTGQIVYKKLKERSEQFVARGLVRTKESKEKINGED-----EVFIGDIRDTA 138

Query: 147 DLDPSIFEGVTHVICCTGTTA------FPSKR------WDGDNTPERVDWEGVRNLISAL 206
            + P++ EG+  ++  T           PSK       +D    PE+VDW G +N I A 
Sbjct: 139 SIAPAV-EGIDALVILTSAVPQMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAA 198

Query: 207 PRS-VKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLPFTIIRHVTLFF 266
             + VK++VLV S+G T  N  P + +    +L +KR+ E +L DSG+P+TIIR      
Sbjct: 199 KAAGVKQIVLVGSMGGTNINH-PLNSIGNANILVWKRKAEQYLADSGIPYTIIR------ 258

Query: 267 NIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIV--- 326
                        AG L D                G  R +L+G+ D+L+   +R +   
Sbjct: 259 -------------AGGLQD--------------KDGGIRELLVGKDDELLETETRTIARA 318

Query: 327 -VAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAAT 353
            VAE C+QAL ++  + +A ++ S + EG G   + +  LF   T
Sbjct: 319 DVAEVCVQALQLEEAKFKALDLAS-KPEGTGTPTKDFKALFTQVT 322

BLAST of Carg25431 vs. ExPASy Swiss-Prot
Match: Q8SKU2 (Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 3.5e-16
Identity = 79/250 (31.60%), Postives = 122/250 (48.80%), Query Frame = 0

Query: 11  PEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVINQEVVQSPSS 70
           P  HL L  +   F R PL       + ++N+IR S S+    S++IR       +S ++
Sbjct: 27  PSPHLNLSKY-SHFMRYPL-----TTTLTNNRIRSSSSS----SSSIRAQASGSTKSSTA 86

Query: 71  ESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEKATTLF----- 130
           E I E KTD      LV V GA+G VG      L+    K+R  +R+ +KA  L      
Sbjct: 87  EGIPE-KTDS-KDDNLVFVAGATGKVGSRTVRELIKLGFKVRAGVRNAQKAGALVQSVKQ 146

Query: 131 --------GEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTP 190
                   G +  + L++ + D    + +  ++    T VIC  G +    K       P
Sbjct: 147 LKLDGASGGGEAVEKLEIVECDLEKADQIGSALGNAST-VICAIGAS---EKEIFDITGP 206

Query: 191 ERVDWEGVRNLI-SALPRSVKRVVLVSSIGVTKFNELPWSIMNLF-GVLKYKRQGEDFLR 246
            R+D+   +NL+ +A    V   +LV+S+G  KF  LP +I+NLF GVL +KR+ E+ L 
Sbjct: 207 CRIDYRATKNLVDAATVAKVNHFILVTSLGTNKFG-LPAAILNLFWGVLIWKRKAEEALL 259

BLAST of Carg25431 vs. ExPASy Swiss-Prot
Match: Q94EG6 (Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 PE=1 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 1.3e-15
Identity = 84/293 (28.67%), Postives = 132/293 (45.05%), Query Frame = 0

Query: 81  VASSKLVLVVGASGGVGQLVAAALLDRNVKL--RLLLRDPEKATTLFGEQDEDMLQVSKG 140
           +A+   VLV GASG  GQ+V   L + + K   + L+R  +    + GE D  +  ++  
Sbjct: 1   MANLPTVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEADVFIGDITDA 60

Query: 141 DTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNT--------------PERVDWEG 200
           D+ NP       F+G+  ++    T+A P  +   D T              PE+VDW G
Sbjct: 61  DSINP------AFQGIDALVIL--TSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIG 120

Query: 201 VRNLISALP-RSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLPFTI 260
            +N I A     VK +V+V S+G T   + P + +    +L +KR+ E +L DSG P+TI
Sbjct: 121 QKNQIDAAKVAGVKHIVVVGSMGGTN-PDHPLNKLGNGNILVWKRKAEQYLADSGTPYTI 180

Query: 261 IRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGE 320
           IR                   AG L D                G  R +L+G+ D+L+  
Sbjct: 181 IR-------------------AGGLLD--------------KEGGVRELLVGKDDELLQT 240

Query: 321 ASRIV----VAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAAT 353
            ++ V    VAE CIQAL  +  + +A+++ S + EG     + +  LF   T
Sbjct: 241 DTKTVPRADVAEVCIQALLFEEAKNKAFDLGS-KPEGTSTPTKDFKALFSQVT 250

BLAST of Carg25431 vs. ExPASy Swiss-Prot
Match: D5JWB3 (Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1)

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-14
Identity = 92/311 (29.58%), Postives = 138/311 (44.37%), Query Frame = 0

Query: 82  ASSKL-VLVVGASGGVGQLVAAALLDRNVK--LRLLLRDPEKATTLFGEQDEDMLQVSKG 141
           +S KL VL+ GASG  G L    L +R+ K  +R L+R  E +    G  DE  +    G
Sbjct: 4   SSKKLTVLLSGASGLTGSLAFKKLKERSDKFEVRGLVRS-EASKQKLGGGDEIFI----G 63

Query: 142 DTRNPEDLDPSIFEGVTHVICCTG-----------------------------TTAFPSK 201
           D  +P+ L+P++ EG+  +I  T                              +   P  
Sbjct: 64  DISDPKTLEPAM-EGIDALIILTSAIPRMKPTEEFTAEMISGGRSEDVIDASFSGPMPEF 123

Query: 202 RWDGDNTPERVDWEGVRNLI-SALPRSVKRVVLVSSIGVTKFNELPWSIMNLFG---VLK 261
            +D    PE+VDW G +N I +A    VK +VLV S+G       P   +N  G   +L 
Sbjct: 124 YYDEGQYPEQVDWIGQKNQIDTAKKMGVKHIVLVGSMGGCD----PDHFLNHMGNGNILI 183

Query: 262 YKRQGEDFLRDSGLPFTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKAT 321
           +KR+ E +L DSG+P+TIIR                   AG L +               
Sbjct: 184 WKRKAEQYLADSGVPYTIIR-------------------AGGLDN--------------K 243

Query: 322 AGQRRAVLLGQGDKLV----GEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDP 353
           AG  R +L+ + D L+    G  +R  VAEAC+QAL+I+  + +A+++ S + EG G   
Sbjct: 244 AGGVRELLVAKDDVLLPTENGFIARADVAEACVQALEIEEVKNKAFDLGS-KPEGVGEAT 270

BLAST of Carg25431 vs. ExPASy Swiss-Prot
Match: Q8H0U5 (Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 6.1e-13
Identity = 54/175 (30.86%), Postives = 88/175 (50.29%), Query Frame = 0

Query: 86  LVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEKATTLF-------------GEQDEDM 145
           LV V GA+G VG      LL    ++R  +R  ++A +L              G Q  + 
Sbjct: 83  LVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEK 142

Query: 146 LQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERVDWEGVRNLI-SA 205
           L++ + D    + + P++    + +ICC G +    K       P R+D+   +NL+ +A
Sbjct: 143 LEIVECDLEKKDSIQPAL-GNASVIICCIGAS---EKEISDITGPYRIDYLATKNLVDAA 202

Query: 206 LPRSVKRVVLVSSIGVTKFNELPWSIMNLF-GVLKYKRQGEDFLRDSGLPFTIIR 246
               V   +LV+S+G  KF   P +I+NLF GVL +KR+ E+ L +SGL + I+R
Sbjct: 203 TSAKVNNFILVTSLGTNKFG-FPAAILNLFWGVLCWKRKAEEALIESGLNYAIVR 252

BLAST of Carg25431 vs. ExPASy TrEMBL
Match: A0A6J1EPG9 (uncharacterized protein At2g37660, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111435394 PE=4 SV=1)

HSP 1 Score: 653.3 bits (1684), Expect = 5.8e-184
Identity = 333/353 (94.33%), Postives = 333/353 (94.33%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI
Sbjct: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVRKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. ExPASy TrEMBL
Match: A0A6J1KUJ0 (uncharacterized protein At2g37660, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111497739 PE=4 SV=1)

HSP 1 Score: 642.5 bits (1656), Expect = 1.0e-180
Identity = 328/353 (92.92%), Postives = 330/353 (93.48%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MASNLSLSFIPEFH PLGNFHKTFQRVPLFGLHPLCSFS+ QIRKSIS+CRGRSAAIR I
Sbjct: 1   MASNLSLSFIPEFHRPLGNFHKTFQRVPLFGLHPLCSFSNYQIRKSISSCRGRSAAIRAI 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
           NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           ATTLFGEQDEDMLQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV
Sbjct: 121 ATTLFGEQDEDMLQVRKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP
Sbjct: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 241 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 354
           VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ
Sbjct: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAATQ 334

BLAST of Carg25431 vs. ExPASy TrEMBL
Match: A0A5D3DW84 (Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold225G001030 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.0e-159
Identity = 296/351 (84.33%), Postives = 311/351 (88.60%), Query Frame = 0

Query: 3   SNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFS--DNQIRKSISNCRGRSAAIRVI 62
           +NLSLS+IP F  P   FH TFQ +PLF L PLCS S  DNQ+R SIS+ R RS AIR I
Sbjct: 102 TNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPAIRAI 161

Query: 63  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 122
           NQEVVQ+PSSES R+SKTDPVASSKLVLVVG SGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 162 NQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEK 221

Query: 123 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 182
           ATTLFGEQDE++LQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPS+RWDGDNTPERV
Sbjct: 222 ATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERV 281

Query: 183 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 242
           DWEGVRNLISA+PRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYK+QGEDFLRDSGLP
Sbjct: 282 DWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLP 341

Query: 243 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 302
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 342 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 401

Query: 303 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAA 352
           VGEASRIVVAEACIQALDI+FTEGQAYEINS+EGEGPGNDP+KWSELFRAA
Sbjct: 402 VGEASRIVVAEACIQALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAA 433

BLAST of Carg25431 vs. ExPASy TrEMBL
Match: A0A5A7VJ33 (Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00410 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.0e-159
Identity = 296/351 (84.33%), Postives = 311/351 (88.60%), Query Frame = 0

Query: 3   SNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFS--DNQIRKSISNCRGRSAAIRVI 62
           +NLSLS+IP F  P   FH TFQ +PLF L PLCS S  DNQ+R SIS+ R RS AIR I
Sbjct: 5   TNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPAIRAI 64

Query: 63  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 122
           NQEVVQ+PSSES R+SKTDPVASSKLVLVVG SGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 65  NQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEK 124

Query: 123 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 182
           ATTLFGEQDE++LQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPS+RWDGDNTPERV
Sbjct: 125 ATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERV 184

Query: 183 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 242
           DWEGVRNLISA+PRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYK+QGEDFLRDSGLP
Sbjct: 185 DWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLP 244

Query: 243 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 302
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 245 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 304

Query: 303 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAA 352
           VGEASRIVVAEACIQALDI+FTEGQAYEINS+EGEGPGNDP+KWSELFRAA
Sbjct: 305 VGEASRIVVAEACIQALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAA 336

BLAST of Carg25431 vs. ExPASy TrEMBL
Match: A0A1S3BPR9 (uncharacterized protein At5g02240 OS=Cucumis melo OX=3656 GN=LOC103492002 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 1.0e-159
Identity = 296/351 (84.33%), Postives = 311/351 (88.60%), Query Frame = 0

Query: 3   SNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFS--DNQIRKSISNCRGRSAAIRVI 62
           +NLSLS+IP F  P   FH TFQ +PLF L PLCS S  DNQ+R SIS+ R RS AIR I
Sbjct: 5   TNLSLSYIPRFPHPPTIFHTTFQTLPLFTLPPLCSSSSFDNQLRNSISSYRLRSPAIRAI 64

Query: 63  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 122
           NQEVVQ+PSSES R+SKTDPVASSKLVLVVG SGGVGQLVAAALLDRNVKLRLLLRDPEK
Sbjct: 65  NQEVVQTPSSESTRDSKTDPVASSKLVLVVGGSGGVGQLVAAALLDRNVKLRLLLRDPEK 124

Query: 123 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 182
           ATTLFGEQDE++LQV KGDTRNPEDLDPSIFEGVTHVICCTGTTAFPS+RWDGDNTPERV
Sbjct: 125 ATTLFGEQDENLLQVCKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSRRWDGDNTPERV 184

Query: 183 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 242
           DWEGVRNLISA+PRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYK+QGEDFLRDSGLP
Sbjct: 185 DWEGVRNLISAIPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKKQGEDFLRDSGLP 244

Query: 243 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 302
           FTIIR                   AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL
Sbjct: 245 FTIIR-------------------AGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 304

Query: 303 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAA 352
           VGEASRIVVAEACIQALDI+FTEGQAYEINS+EGEGPGNDP+KWSELFRAA
Sbjct: 305 VGEASRIVVAEACIQALDINFTEGQAYEINSIEGEGPGNDPKKWSELFRAA 336

BLAST of Carg25431 vs. TAIR 10
Match: AT4G31530.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 414.5 bits (1064), Expect = 8.8e-116
Identity = 223/351 (63.53%), Postives = 262/351 (74.64%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MA+  +LSF P  +       K+  R PLF   PL   S   + ++ ++       ++  
Sbjct: 1   MATTTNLSFAPPSYSRFA-ATKSQIRNPLF-TSPLPLPSSFFLVRNEASLSSSITPVQAF 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
            +E V +    S         +SSKLVLVVG +GGVGQLV A+LL RN++ RLLLRD +K
Sbjct: 61  TEEAVDTSDLAS---------SSSKLVLVVGGTGGVGQLVVASLLKRNIRSRLLLRDLDK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           AT LFG+QDE  LQV KGDTRN EDLDPS+FEGVTHVIC TGTTAFPSKRW+ +NTPE+V
Sbjct: 121 ATKLFGKQDEYSLQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEENTPEKV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGV+NLISALP SVKRVVLVSS+GVTK NELPWSIMNLFGVLKYK+ GEDFLRDSGLP
Sbjct: 181 DWEGVKNLISALPSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                    GRLTDGPYTSYDLNTLLKATAG+RRAV++GQGD L
Sbjct: 241 FTIIR-------------------PGRLTDGPYTSYDLNTLLKATAGERRAVVIGQGDNL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAA 352
           VGE SR+VVAEACIQALDI+FT+G+AYEINSV+G+GPG+DPQ+W ELF+AA
Sbjct: 301 VGEVSRLVVAEACIQALDIEFTQGKAYEINSVKGDGPGSDPQQWRELFKAA 321

BLAST of Carg25431 vs. TAIR 10
Match: AT4G31530.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 404.8 bits (1039), Expect = 7.0e-113
Identity = 223/365 (61.10%), Postives = 262/365 (71.78%), Query Frame = 0

Query: 1   MASNLSLSFIPEFHLPLGNFHKTFQRVPLFGLHPLCSFSDNQIRKSISNCRGRSAAIRVI 60
           MA+  +LSF P  +       K+  R PLF   PL   S   + ++ ++       ++  
Sbjct: 1   MATTTNLSFAPPSYSRFA-ATKSQIRNPLF-TSPLPLPSSFFLVRNEASLSSSITPVQAF 60

Query: 61  NQEVVQSPSSESIRESKTDPVASSKLVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEK 120
            +E V +    S         +SSKLVLVVG +GGVGQLV A+LL RN++ RLLLRD +K
Sbjct: 61  TEEAVDTSDLAS---------SSSKLVLVVGGTGGVGQLVVASLLKRNIRSRLLLRDLDK 120

Query: 121 ATTLFGEQDEDMLQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERV 180
           AT LFG+QDE  LQV KGDTRN EDLDPS+FEGVTHVIC TGTTAFPSKRW+ +NTPE+V
Sbjct: 121 ATKLFGKQDEYSLQVVKGDTRNAEDLDPSMFEGVTHVICTTGTTAFPSKRWNEENTPEKV 180

Query: 181 DWEGVRNLISALPRSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLP 240
           DWEGV+NLISALP SVKRVVLVSS+GVTK NELPWSIMNLFGVLKYK+ GEDFLRDSGLP
Sbjct: 181 DWEGVKNLISALPSSVKRVVLVSSVGVTKSNELPWSIMNLFGVLKYKKMGEDFLRDSGLP 240

Query: 241 FTIIRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKL 300
           FTIIR                    GRLTDGPYTSYDLNTLLKATAG+RRAV++GQGD L
Sbjct: 241 FTIIR-------------------PGRLTDGPYTSYDLNTLLKATAGERRAVVIGQGDNL 300

Query: 301 VGEASRIVVAEACIQALDIDFTEGQAYEINSVE--------------GEGPGNDPQKWSE 352
           VGE SR+VVAEACIQALDI+FT+G+AYEINSV+              G+GPG+DPQ+W E
Sbjct: 301 VGEVSRLVVAEACIQALDIEFTQGKAYEINSVKVPQTVSLKFEQSGNGDGPGSDPQQWRE 335

BLAST of Carg25431 vs. TAIR 10
Match: AT2G37660.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 5.3e-20
Identity = 87/285 (30.53%), Postives = 133/285 (46.67%), Query Frame = 0

Query: 87  VLVVGASGGVGQLVAAALLDRNVKL--RLLLRDPEKATTLFGEQDEDMLQVSKGDTRNPE 146
           VLV GA G  GQ+V   L +R+ +   R L+R  E    + GE      +V  GD R+  
Sbjct: 79  VLVTGAGGRTGQIVYKKLKERSEQFVARGLVRTKESKEKINGED-----EVFIGDIRDTA 138

Query: 147 DLDPSIFEGVTHVICCTGTTA------FPSKR------WDGDNTPERVDWEGVRNLISAL 206
            + P++ EG+  ++  T           PSK       +D    PE+VDW G +N I A 
Sbjct: 139 SIAPAV-EGIDALVILTSAVPQMKPGFDPSKGGRPEFFFDDGAYPEQVDWIGQKNQIDAA 198

Query: 207 PRS-VKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLPFTIIRHVTLFF 266
             + VK++VLV S+G T  N  P + +    +L +KR+ E +L DSG+P+TIIR      
Sbjct: 199 KAAGVKQIVLVGSMGGTNINH-PLNSIGNANILVWKRKAEQYLADSGIPYTIIR------ 258

Query: 267 NIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGEASRIV--- 326
                        AG L D                G  R +L+G+ D+L+   +R +   
Sbjct: 259 -------------AGGLQD--------------KDGGIRELLVGKDDELLETETRTIARA 318

Query: 327 -VAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAAT 353
            VAE C+QAL ++  + +A ++ S + EG G   + +  LF   T
Sbjct: 319 DVAEVCVQALQLEEAKFKALDLAS-KPEGTGTPTKDFKALFTQVT 322

BLAST of Carg25431 vs. TAIR 10
Match: AT5G02240.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 85.5 bits (210), Expect = 9.3e-17
Identity = 84/293 (28.67%), Postives = 132/293 (45.05%), Query Frame = 0

Query: 81  VASSKLVLVVGASGGVGQLVAAALLDRNVKL--RLLLRDPEKATTLFGEQDEDMLQVSKG 140
           +A+   VLV GASG  GQ+V   L + + K   + L+R  +    + GE D  +  ++  
Sbjct: 1   MANLPTVLVTGASGRTGQIVYKKLKEGSDKFVAKGLVRSAQGKEKIGGEADVFIGDITDA 60

Query: 141 DTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNT--------------PERVDWEG 200
           D+ NP       F+G+  ++    T+A P  +   D T              PE+VDW G
Sbjct: 61  DSINP------AFQGIDALVIL--TSAVPKMKPGFDPTKGGRPEFIFEDGQYPEQVDWIG 120

Query: 201 VRNLISALP-RSVKRVVLVSSIGVTKFNELPWSIMNLFGVLKYKRQGEDFLRDSGLPFTI 260
            +N I A     VK +V+V S+G T   + P + +    +L +KR+ E +L DSG P+TI
Sbjct: 121 QKNQIDAAKVAGVKHIVVVGSMGGTN-PDHPLNKLGNGNILVWKRKAEQYLADSGTPYTI 180

Query: 261 IRHVTLFFNIEHPLSLLCTKLAGRLTDGPYTSYDLNTLLKATAGQRRAVLLGQGDKLVGE 320
           IR                   AG L D                G  R +L+G+ D+L+  
Sbjct: 181 IR-------------------AGGLLD--------------KEGGVRELLVGKDDELLQT 240

Query: 321 ASRIV----VAEACIQALDIDFTEGQAYEINSVEGEGPGNDPQKWSELFRAAT 353
            ++ V    VAE CIQAL  +  + +A+++ S + EG     + +  LF   T
Sbjct: 241 DTKTVPRADVAEVCIQALLFEEAKNKAFDLGS-KPEGTSTPTKDFKALFSQVT 250

BLAST of Carg25431 vs. TAIR 10
Match: AT3G18890.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 76.6 bits (187), Expect = 4.3e-14
Identity = 54/175 (30.86%), Postives = 88/175 (50.29%), Query Frame = 0

Query: 86  LVLVVGASGGVGQLVAAALLDRNVKLRLLLRDPEKATTLF-------------GEQDEDM 145
           LV V GA+G VG      LL    ++R  +R  ++A +L              G Q  + 
Sbjct: 83  LVFVAGATGKVGSRTVRELLKLGFRVRAGVRSAQRAGSLVQSVKEMKLQNTDEGTQPVEK 142

Query: 146 LQVSKGDTRNPEDLDPSIFEGVTHVICCTGTTAFPSKRWDGDNTPERVDWEGVRNLI-SA 205
           L++ + D    + + P++    + +ICC G +    K       P R+D+   +NL+ +A
Sbjct: 143 LEIVECDLEKKDSIQPAL-GNASVIICCIGAS---EKEISDITGPYRIDYLATKNLVDAA 202

Query: 206 LPRSVKRVVLVSSIGVTKFNELPWSIMNLF-GVLKYKRQGEDFLRDSGLPFTIIR 246
               V   +LV+S+G  KF   P +I+NLF GVL +KR+ E+ L +SGL + I+R
Sbjct: 203 TSAKVNNFILVTSLGTNKFG-FPAAILNLFWGVLCWKRKAEEALIESGLNYAIVR 252

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022102.11.6e-199100.00putative protein, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6588205.13.2e-18494.62putative protein, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022928583.11.2e-18394.33uncharacterized protein At2g37660, chloroplastic [Cucurbita moschata][more]
XP_023530592.12.5e-18193.20uncharacterized protein At2g37660, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_023004425.12.1e-18092.92uncharacterized protein At2g37660, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
O809347.5e-1930.53Uncharacterized protein At2g37660, chloroplastic OS=Arabidopsis thaliana OX=3702... [more]
Q8SKU23.5e-1631.60Protein TIC 62, chloroplastic OS=Pisum sativum OX=3888 GN=TIC62 PE=1 SV=2[more]
Q94EG61.3e-1528.67Uncharacterized protein At5g02240 OS=Arabidopsis thaliana OX=3702 GN=At5g02240 P... [more]
D5JWB31.5e-1429.58Sanguinarine reductase OS=Eschscholzia californica OX=3467 GN=SARED1 PE=1 SV=1[more]
Q8H0U56.1e-1330.86Protein TIC 62, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC62 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EPG95.8e-18494.33uncharacterized protein At2g37660, chloroplastic OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1KUJ01.0e-18092.92uncharacterized protein At2g37660, chloroplastic OS=Cucurbita maxima OX=3661 GN=... [more]
A0A5D3DW841.0e-15984.33Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E567... [more]
A0A5A7VJ331.0e-15984.33Sanguinarine reductase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2... [more]
A0A1S3BPR91.0e-15984.33uncharacterized protein At5g02240 OS=Cucumis melo OX=3656 GN=LOC103492002 PE=4 S... [more]
Match NameE-valueIdentityDescription
AT4G31530.18.8e-11663.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT4G31530.27.0e-11361.10NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G37660.15.3e-2030.53NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G02240.19.3e-1728.67NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G18890.14.3e-1430.86NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016040NAD(P)-binding domainPFAMPF13460NAD_binding_10coord: 91..250
e-value: 2.6E-24
score: 86.1
NoneNo IPR availableGENE3D3.40.50.720coord: 82..352
e-value: 5.6E-52
score: 178.7
NoneNo IPR availablePANTHERPTHR10366:SF384NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 72..350
NoneNo IPR availablePANTHERPTHR10366NAD DEPENDENT EPIMERASE/DEHYDRATASEcoord: 72..350
NoneNo IPR availableCDDcd05243SDR_a5coord: 87..329
e-value: 6.11466E-55
score: 177.043
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 85..330

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25431-RACarg25431-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor