Carg25355 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25355 vs. NCBI nr
Match: KAG7028414.1 (Rab3 GTPase-activating protein catalytic subunit, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540

Query: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 584
           ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV
Sbjct: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 583

BLAST of Carg25355 vs. NCBI nr
Match: XP_022949616.1 (rab3 GTPase-activating protein catalytic subunit-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 577/583 (98.97%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLL SSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLDSSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFL+R+ITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLKRSITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSS RNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSPRNANSD 540

Query: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 584
           ERLSSSTQ+FSSFFDSKSSL AKKPPKPESTSATPVENGWTLV
Sbjct: 541 ERLSSSTQSFSSFFDSKSSLFAKKPPKPESTSATPVENGWTLV 583

BLAST of Carg25355 vs. NCBI nr
Match: XP_023521287.1 (rab3 GTPase-activating protein catalytic subunit-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023521288.1 rab3 GTPase-activating protein catalytic subunit-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1120.5 bits (2897), Expect = 0.0e+00
Identity = 576/583 (98.80%), Postives = 579/583 (99.31%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLLASSAGSSS KGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLASSAGSSSAKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFL+R+ITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLKRSITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSP+TQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPVTQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSS RNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSPRNANSD 540

Query: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 584
           ERLSSSTQ FSSFFDSKSSL AKKPPKPESTSATPVENGWTLV
Sbjct: 541 ERLSSSTQPFSSFFDSKSSLFAKKPPKPESTSATPVENGWTLV 583

BLAST of Carg25355 vs. NCBI nr
Match: KAG6596934.1 (Rab3 GTPase-activating protein catalytic subunit, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1102.8 bits (2851), Expect = 0.0e+00
Identity = 569/569 (100.00%), Postives = 569/569 (100.00%), Query Frame = 0

Query: 15  MKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKSKSVKSLLASSAGSS 74
           MKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKSKSVKSLLASSAGSS
Sbjct: 1   MKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKSKSVKSLLASSAGSS 60

Query: 75  SGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAGGDFLRRNITSGSEG 134
           SGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAGGDFLRRNITSGSEG
Sbjct: 61  SGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAGGDFLRRNITSGSEG 120

Query: 135 TIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEMRRFWSEEQYIPGIP 194
           TIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEMRRFWSEEQYIPGIP
Sbjct: 121 TIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEMRRFWSEEQYIPGIP 180

Query: 195 IDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSNTESGTSEVTVPANS 254
           IDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSNTESGTSEVTVPANS
Sbjct: 181 IDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSNTESGTSEVTVPANS 240

Query: 255 LLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTG 314
           LLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTG
Sbjct: 241 LLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTG 300

Query: 315 SVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLS 374
           SVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLS
Sbjct: 301 SVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLS 360

Query: 375 SRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLPTTELYGPLFLSLLG 434
           SRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLPTTELYGPLFLSLLG
Sbjct: 361 SRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLPTTELYGPLFLSLLG 420

Query: 435 LGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQVYETVETMMVNPDEI 494
           LGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQVYETVETMMVNPDEI
Sbjct: 421 LGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQVYETVETMMVNPDEI 480

Query: 495 LKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSDERLSSSTQTFSSFF 554
           LKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSDERLSSSTQTFSSFF
Sbjct: 481 LKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSDERLSSSTQTFSSFF 540

Query: 555 DSKSSLLAKKPPKPESTSATPVENGWTLV 584
           DSKSSLLAKKPPKPESTSATPVENGWTLV
Sbjct: 541 DSKSSLLAKKPPKPESTSATPVENGWTLV 569

BLAST of Carg25355 vs. NCBI nr
Match: XP_023005364.1 (rab3 GTPase-activating protein catalytic subunit-like [Cucurbita maxima])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.6e-304
Identity = 541/550 (98.36%), Postives = 545/550 (99.09%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLLASSAGSSS KGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLASSAGSSSAKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFL+R+ITSGSEGTIAASLPRDIHGAPP+SLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLKRSITSGSEGTIAASLPRDIHGAPPESLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSP+TQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPVTQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKL EDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLTEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKK S RNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKWSPRNANSD 540

Query: 541 ERLSSSTQTF 551
           ERLSSSTQ F
Sbjct: 541 ERLSSSTQPF 550

BLAST of Carg25355 vs. ExPASy Swiss-Prot
Match: Q80UJ7 (Rab3 GTPase-activating protein catalytic subunit OS=Mus musculus OX=10090 GN=Rab3gap1 PE=1 SV=4)

HSP 1 Score: 148.3 bits (373), Expect = 2.7e-34
Identity = 106/329 (32.22%), Postives = 154/329 (46.81%), Query Frame = 0

Query: 139 SLPRDIHGAPPDSLLVKISEVIGSFR----SLRKMTLFWCRIVDEMRRFWSEEQYIPGIP 198
           +L      AP DSL  K++  +         L+ +   W   V EMR  W     IPG+ 
Sbjct: 442 TLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLA 501

Query: 199 IDEIPDLNSCLLYQRFQVINCCLSRKRRHE-----IASDS-----------------LDV 258
               PDL  CLL+Q+ Q++NCC+ RK+  +       SDS                 LD 
Sbjct: 502 SGS-PDLRCCLLHQKLQMLNCCIERKKARDEGKKTSLSDSTTSAYPGDAGKTGGQLGLDH 561

Query: 259 VLRVASSNTESGTS--------EVTVPANSLLYARLNNGELALRLGADCPFSN------- 318
           +        E G S        E      S       NG+ + + G     +N       
Sbjct: 562 LRDTEKEKGEVGKSWDSWSDSEEEFFECLSDTEDLKGNGQESGKKGGPKEMANLKPEGRL 621

Query: 319 -----LTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTGSVGAGC--------SQLL 378
                LT+L  GE +Y P+TQE   +TED+++E  E + + G+   G         + LL
Sbjct: 622 HQHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLL 681

Query: 379 SDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLSSRMQKEGNLW 414
           SDM++FKAANPGC LEDFVRW+SP D+ E E ++   +  +    +G+LS+RM+   N+W
Sbjct: 682 SDMESFKAANPGCFLEDFVRWYSPRDYIEEEVTDEKGNVVL----KGELSARMKIPSNMW 741

BLAST of Carg25355 vs. ExPASy Swiss-Prot
Match: P69735 (Rab3 GTPase-activating protein catalytic subunit (Fragments) OS=Rattus norvegicus OX=10116 GN=Rab3gap1 PE=1 SV=2)

HSP 1 Score: 147.5 bits (371), Expect = 4.7e-34
Identity = 105/329 (31.91%), Postives = 154/329 (46.81%), Query Frame = 0

Query: 139 SLPRDIHGAPPDSLLVKISEVIGSFR----SLRKMTLFWCRIVDEMRRFWSEEQYIPGIP 198
           +L      AP DSL  K++  +         L+ +   W   V EMR  W     IPG+ 
Sbjct: 236 TLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLA 295

Query: 199 IDEIPDLNSCLLYQRFQVINCCLSRKRRHE-----IASDS-----------------LDV 258
               PDL  CLL+Q+ Q++NCC+ RK+  +       SDS                 LD 
Sbjct: 296 SGP-PDLRCCLLHQKLQMLNCCIERKKARDEGKKTSPSDSMTKAYPADAGKAGGQLGLDH 355

Query: 259 VLRVASSNTESGTS--------EVTVPANSLLYARLNNGELALRLGADCPFS-------- 318
           +        E+G S        E      S       NG+ + + G     +        
Sbjct: 356 LRDTEKEKGEAGKSWDSWSDSEEEFFECLSDAEDLRGNGQESTKKGGPKDMAPLKPEGRL 415

Query: 319 ----NLTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTGSVGAGC--------SQLL 378
                LT+L  GE +Y P+TQE   +TED+++E  E + + G+   G         + LL
Sbjct: 416 HQHGKLTLLRNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLL 475

Query: 379 SDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLSSRMQKEGNLW 414
           SDM++FKAANPGC LEDFVRW+SP D+ E E ++   +  +    +G+LS+RM+   N+W
Sbjct: 476 SDMESFKAANPGCCLEDFVRWYSPRDYIEEEVTDEKGNVVL----KGELSARMKIPSNMW 535

BLAST of Carg25355 vs. ExPASy Swiss-Prot
Match: Q15042 (Rab3 GTPase-activating protein catalytic subunit OS=Homo sapiens OX=9606 GN=RAB3GAP1 PE=1 SV=3)

HSP 1 Score: 146.0 bits (367), Expect = 1.4e-33
Identity = 103/328 (31.40%), Postives = 155/328 (47.26%), Query Frame = 0

Query: 139 SLPRDIHGAPPDSLLVKISEVIGSFR----SLRKMTLFWCRIVDEMRRFWSEEQYIPGIP 198
           +L      AP DSL  K++  +         L+ +   W   V EMR  W     IPG+ 
Sbjct: 443 NLYNQFKSAPSDSLTYKLALCLCMINFYHGGLKGVAHLWQEFVLEMRFRWENNFLIPGLA 502

Query: 199 IDEIPDLNSCLLYQRFQVINCCLSRKRRHE-----IASDSLDVVLRVA------------ 258
               PDL  CLL+Q+ Q++NCC+ RK+  +      ASD  ++    A            
Sbjct: 503 SGP-PDLRCCLLHQKLQMLNCCIERKKARDEGKKTSASDVTNIYPGDAGKAGDQLVPDNL 562

Query: 259 ------------SSNTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSN-------- 318
                       S ++ S + E      S       NG+ + + G     +N        
Sbjct: 563 KETDKEKGEVGKSWDSWSDSEEEFFECLSDTEELKGNGQESGKKGGPKEMANLRPEGRLY 622

Query: 319 ----LTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTGSVGAGC--------SQLLS 378
               LT+L  GE +Y P+TQE   +TED+++E  E + + G+   G         + LLS
Sbjct: 623 QHGKLTLLHNGEPLYIPVTQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLS 682

Query: 379 DMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLSSRMQKEGNLWR 414
           DM++FKAANPGC LEDFVRW+SP D+ E E  +   +  +    +G+LS+RM+   N+W 
Sbjct: 683 DMESFKAANPGCSLEDFVRWYSPRDYIEEEVIDEKGNVVL----KGELSARMKIPSNMWV 742

BLAST of Carg25355 vs. ExPASy Swiss-Prot
Match: Q642R9 (Rab3 GTPase-activating protein catalytic subunit OS=Xenopus laevis OX=8355 GN=rab3gap1 PE=2 SV=1)

HSP 1 Score: 146.0 bits (367), Expect = 1.4e-33
Identity = 107/338 (31.66%), Postives = 164/338 (48.52%), Query Frame = 0

Query: 127 NITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFR----SLRKMTLFWCRIVDEMRR 186
           NI+S SE     +L   +  AP +SL  K++  +         ++ +   W   V EMR 
Sbjct: 433 NISSQSESE-DYNLYSQLKSAPSNSLTYKLALCLCMVNFYHGGVKGVAHLWQEFVLEMRY 492

Query: 187 FWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRR------------------HE 246
            W     IPG+  +  PDL  CLL+Q+ Q++NCCL RK+                   ++
Sbjct: 493 RWENNFLIPGL-ANGSPDLKCCLLHQKLQMLNCCLERKKARDEGKKGNPLYSSSESSVNK 552

Query: 247 IASDSLDVV------LRVASS-NTESGTSEVTVPANSLLYARLNNGELALRL-------- 306
            ASD L  V        VA S ++ S + E     +S       +G+ + R         
Sbjct: 553 TASDLLSPVEADKSKYEVAKSWDSWSDSEEEFFECHSDTEELKESGQESARKAKEETKEN 612

Query: 307 ------GADCPFSNLTMLETGEKVYSPITQEGPLLTEDVIKETEEFVLRTGSVGAGC--- 366
                 G      NL +L +GE +Y P+TQ+   +T+D+++E  E + + G+   G    
Sbjct: 613 PSPKPEGRLHQSGNLMLLNSGEPLYIPVTQDPAPMTDDLLEEQSEVLAKLGTSAEGAHLR 672

Query: 367 -----SQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLSS 414
                + LLSDM++FKAANPGC LEDFVRW+SP D+ E E  +   +       +G+LS+
Sbjct: 673 ARMQSACLLSDMESFKAANPGCCLEDFVRWYSPRDYIEEEVMDDKGNKIF----KGELSA 732

BLAST of Carg25355 vs. ExPASy Swiss-Prot
Match: Q6NUV0 (Rab3 GTPase-activating protein catalytic subunit OS=Danio rerio OX=7955 GN=rab3gap1 PE=2 SV=2)

HSP 1 Score: 144.4 bits (363), Expect = 3.9e-33
Identity = 108/349 (30.95%), Postives = 159/349 (45.56%), Query Frame = 0

Query: 140 LPRDIHGAPPDSLLVKISEVIGSFR----SLRKMTLFWCRIVDEMRRFWSEEQYIPGIPI 199
           L   +   P DSL  +++  I         +R +   W   V EMR  W     I G+  
Sbjct: 445 LYNQLKSCPSDSLTHRLALCICMVNFHHGGVRAVAHLWQEFVLEMRYRWENNCLIYGLAS 504

Query: 200 DEIPDLNSCLLYQRFQVINCCLSRKRRHEIA--SDSLDVVLRVASSNTESGTSEVTVPAN 259
              PDL  CLL+Q+ Q++NCC+ RK+  +    S S D     +    E    E   PA 
Sbjct: 505 GP-PDLRCCLLHQKLQMLNCCIERKKARDDGKKSSSSDGARDRSRGAPEGAGPEGAGPAE 564

Query: 260 SLLYA--------------------------RLNNGELALRLGADCPFSNLTMLETGEKV 319
           +   +                             N   +   G   P    T+L T E +
Sbjct: 565 AAGKSWDSWSDSEDEFFECVSDTEEMKEDKEEAENRSRSKPEGRLQPHGTHTLLNTQEPL 624

Query: 320 YSPITQEGPLLTEDVIKETEEFVLRTGSVGAGC--------SQLLSDMQAFKAANPGCIL 379
           Y PITQE   +TED+++E  E + + G+   G         + LLSDM++FKAANPGC L
Sbjct: 625 YIPITQEPAPMTEDLLEEQSEVLAKLGTSAEGAHLRARMQSACLLSDMESFKAANPGCTL 684

Query: 380 EDFVRWHSPPDWTEPEPSNGSVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQ 439
            DFVRW+SP D+ E + ++      +    RG+LS+RM+  GN+W E W+T++A PA +Q
Sbjct: 685 LDFVRWYSPRDYVEEQVTDADGRVEV----RGELSARMKIPGNMWVEAWETARATPARRQ 744

Query: 440 TPLFDEDLVVEGILTDLEDLPTTELYGPLFLSLLGLGFV-MAEAKLAED 448
             LFD+    E +L  L     +EL   L   +L    + + E + AED
Sbjct: 745 KRLFDDTKEAEKVLHYLALQKPSELTRHLLPCVLHAALLKIKEEESAED 788

BLAST of Carg25355 vs. ExPASy TrEMBL
Match: A0A6J1GCH2 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111452913 PE=3 SV=1)

HSP 1 Score: 1121.7 bits (2900), Expect = 0.0e+00
Identity = 577/583 (98.97%), Postives = 580/583 (99.49%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLL SSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLDSSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFL+R+ITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLKRSITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSS RNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSPRNANSD 540

Query: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 584
           ERLSSSTQ+FSSFFDSKSSL AKKPPKPESTSATPVENGWTLV
Sbjct: 541 ERLSSSTQSFSSFFDSKSSLFAKKPPKPESTSATPVENGWTLV 583

BLAST of Carg25355 vs. ExPASy TrEMBL
Match: A0A6J1KYY7 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111498379 PE=3 SV=1)

HSP 1 Score: 1053.5 bits (2723), Expect = 3.2e-304
Identity = 541/550 (98.36%), Postives = 545/550 (99.09%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS
Sbjct: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KSVKSLLASSAGSSS KGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG
Sbjct: 61  KSVKSLLASSAGSSSAKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           GDFL+R+ITSGSEGTIAASLPRDIHGAPP+SLLVKISEVIGSFRSLRKMTLFWCRIVDEM
Sbjct: 121 GDFLKRSITSGSEGTIAASLPRDIHGAPPESLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN
Sbjct: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
           TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSP+TQEGPLLTE
Sbjct: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPVTQEGPLLTE 300

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS
Sbjct: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP
Sbjct: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
           TTELYGPLFLSLLGLGFVMAEAKL EDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV
Sbjct: 421 TTELYGPLFLSLLGLGFVMAEAKLTEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKK S RNANSD
Sbjct: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKWSPRNANSD 540

Query: 541 ERLSSSTQTF 551
           ERLSSSTQ F
Sbjct: 541 ERLSSSTQPF 550

BLAST of Carg25355 vs. ExPASy TrEMBL
Match: A0A0A0L2W4 (Rab3 GTPase-activating protein catalytic subunit OS=Cucumis sativus OX=3659 GN=Csa_3G064220 PE=3 SV=1)

HSP 1 Score: 952.2 bits (2460), Expect = 1.0e-273
Identity = 486/583 (83.36%), Postives = 522/583 (89.54%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           M+VPFYDENLY+LNMKNDIEAKNAEIIPSVES   TDK SIP LSVIKQLA A+EAGKKS
Sbjct: 105 MAVPFYDENLYLLNMKNDIEAKNAEIIPSVESLWTTDKDSIPPLSVIKQLATAVEAGKKS 164

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           KS+KSLLASS  SS  + K GL+LS+VRAL+LRE+EEK ST FRHDERIQSLI SLFDA 
Sbjct: 165 KSMKSLLASSGDSSPAREKSGLSLSSVRALMLREREEKSSTEFRHDERIQSLICSLFDAE 224

Query: 121 GDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDEM 180
           G FLRR   + SEGT   SLP+DIHGAPPDSLLVKISEVIGSFR+LRKM LFWCRIVDEM
Sbjct: 225 GVFLRRYFDTASEGTFVTSLPKDIHGAPPDSLLVKISEVIGSFRTLRKMALFWCRIVDEM 284

Query: 181 RRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASSN 240
           RRFWSEEQY+PGIPIDEIPDLNSCLLYQR QVINCC+SRKRRHEIA+DS+D  LR ASSN
Sbjct: 285 RRFWSEEQYLPGIPIDEIPDLNSCLLYQRLQVINCCVSRKRRHEIATDSIDAALREASSN 344

Query: 241 TESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLTE 300
            ES TSEVT+P N+LLYARLNNGELALRLGADCPF N  MLETGE VYSP+TQEGPLLTE
Sbjct: 345 AESKTSEVTIPGNTLLYARLNNGELALRLGADCPFGNHKMLETGEAVYSPVTQEGPLLTE 404

Query: 301 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNGS 360
           DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSN S
Sbjct: 405 DVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNDS 464

Query: 361 VHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDLP 420
           + S + SDSRGQLSSRMQKEGNLW ELW+TSK VPAVKQTPLFDEDLVVEGIL DLEDLP
Sbjct: 465 IDSPVGSDSRGQLSSRMQKEGNLWLELWETSKPVPAVKQTPLFDEDLVVEGILNDLEDLP 524

Query: 421 TTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQV 480
            +EL+ PLF+SLLGLGF++AEAKLA +NNLSKLFY+CK YVVATCQ SSW+NKVDD+CQV
Sbjct: 525 PSELFQPLFISLLGLGFIVAEAKLANNNNLSKLFYDCKGYVVATCQNSSWSNKVDDLCQV 584

Query: 481 YETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANSD 540
           YETVETMMVNP+EILKA+KQ EESNMT S LKRRFKKLSLN VGKD Q++KSS RNANSD
Sbjct: 585 YETVETMMVNPEEILKAIKQPEESNMTASELKRRFKKLSLNFVGKDGQSRKSSPRNANSD 644

Query: 541 ERLSSSTQTFSSFFDSKSSLLAKKPPKPESTSATPVENGWTLV 584
           E   SSTQ FSSFFDSKSSL AKKPPKPE+ SATPVENGWT V
Sbjct: 645 ES-PSSTQPFSSFFDSKSSLFAKKPPKPETPSATPVENGWTFV 686

BLAST of Carg25355 vs. ExPASy TrEMBL
Match: A0A6J1EVT2 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita moschata OX=3662 GN=LOC111438594 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.5e-272
Identity = 487/585 (83.25%), Postives = 528/585 (90.26%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLY+LNMKNDIEAKNAEI+PSVESFLATD+ASIP LSVIKQLAIA+EAGKKS
Sbjct: 101 MSVPFYDENLYLLNMKNDIEAKNAEIVPSVESFLATDRASIPPLSVIKQLAIAVEAGKKS 160

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDA- 120
           KS+KSL+AS   SSS + K GL+LS+VRAL+LREKEEK ST FRHDER+QSLI SLFDA 
Sbjct: 161 KSIKSLVASPGDSSSTREKSGLSLSSVRALMLREKEEKSSTEFRHDERVQSLICSLFDAE 220

Query: 121 GGDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDE 180
           GGDFLRRN  + SEGTI  SL RDIHGAPPDSLLVKISEVIGSFR LRKM LFWCRIVDE
Sbjct: 221 GGDFLRRNFGTASEGTIVTSLTRDIHGAPPDSLLVKISEVIGSFRCLRKMALFWCRIVDE 280

Query: 181 MRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASS 240
           MRRFW+EE+Y+PGIPIDEIPDLNSCLLYQRFQVINCC+SRKRRHEIA+D LD VLR ASS
Sbjct: 281 MRRFWAEERYLPGIPIDEIPDLNSCLLYQRFQVINCCVSRKRRHEIATDKLDAVLREASS 340

Query: 241 NTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLT 300
           N ES TSEVTVPAN+LLYARL+NGELALRLGADCPF +  MLETGE VYSP+TQEGPLLT
Sbjct: 341 NAESRTSEVTVPANTLLYARLSNGELALRLGADCPFGDHKMLETGEAVYSPVTQEGPLLT 400

Query: 301 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNG 360
           EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEP+N 
Sbjct: 401 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPNND 460

Query: 361 SVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDL 420
           S+ S +  DSRGQLSSRMQKEGNLWRELW+TSKAVPAVKQTPLFDEDLVVEGIL+DLE+L
Sbjct: 461 SIDSPVGIDSRGQLSSRMQKEGNLWRELWETSKAVPAVKQTPLFDEDLVVEGILSDLEEL 520

Query: 421 PTTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQ 480
           P +EL+ PLF+SLLGLGF+MAEAKLA++NNLS LFYECK YVVATCQ SSW+NKVDDICQ
Sbjct: 521 PPSELFEPLFISLLGLGFIMAEAKLAKNNNLSSLFYECKGYVVATCQRSSWSNKVDDICQ 580

Query: 481 VYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANS 540
           VYETVETMMVNP+EILKAMKQ EESNMT   LKRRFKK+SLN VGKD  ++KSS +N NS
Sbjct: 581 VYETVETMMVNPEEILKAMKQPEESNMTAGELKRRFKKMSLNFVGKDGDSRKSSPKNVNS 640

Query: 541 DERLSSSTQTFSSFFDSKSSLLAKKPPKPESTS-ATPVENGWTLV 584
           DER SS   +FSSFFDSKSSL AKKPPKPE+ S AT  ENGWT V
Sbjct: 641 DERPSS--PSFSSFFDSKSSLFAKKPPKPETPSAATTDENGWTFV 683

BLAST of Carg25355 vs. ExPASy TrEMBL
Match: A0A6J1KZH5 (Rab3 GTPase-activating protein catalytic subunit OS=Cucurbita maxima OX=3661 GN=LOC111500257 PE=3 SV=1)

HSP 1 Score: 946.4 bits (2445), Expect = 5.5e-272
Identity = 488/585 (83.42%), Postives = 528/585 (90.26%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           MSVPFYDENLY+LNMKNDIEAKNAEI+PSVESFLATD+ASIP LSVIKQLAIA+EAGKKS
Sbjct: 101 MSVPFYDENLYLLNMKNDIEAKNAEIVPSVESFLATDRASIPPLSVIKQLAIAVEAGKKS 160

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDA- 120
           KS+KSL+AS   SSS + K GL+LS+VRAL+LREKEEK ST FR DER+QSLI SLFDA 
Sbjct: 161 KSIKSLVASPGDSSSTREKSGLSLSSVRALMLREKEEKSSTEFRLDERVQSLICSLFDAE 220

Query: 121 GGDFLRRNITSGSEGTIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDE 180
           GGDFLRRN  + SEGTI  SL RDIHGAPPDSLLVKISEVIGSFR LRKM LFWCRIVDE
Sbjct: 221 GGDFLRRNFGTASEGTIVTSLTRDIHGAPPDSLLVKISEVIGSFRCLRKMALFWCRIVDE 280

Query: 181 MRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASS 240
           MRRFW+EE+YIPGIPIDEIPDLNSCLLYQRFQVINCC+SRKRRHEIA+D LD VLR ASS
Sbjct: 281 MRRFWAEERYIPGIPIDEIPDLNSCLLYQRFQVINCCVSRKRRHEIATDKLDAVLREASS 340

Query: 241 NTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLT 300
           N ES TSEVTVPAN+LLYARL+NGELALRLGADCPF +  MLETGE VYSP+TQEGPLLT
Sbjct: 341 NAESRTSEVTVPANTLLYARLSNGELALRLGADCPFGDHKMLETGEAVYSPVTQEGPLLT 400

Query: 301 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNG 360
           EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEP+N 
Sbjct: 401 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPNND 460

Query: 361 SVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDL 420
           S+ S +  DSRGQLSSRMQKEGNLWRELW+TSKAVPAVKQTPLFDEDLVVEGIL+DLE+L
Sbjct: 461 SIDSPVGIDSRGQLSSRMQKEGNLWRELWETSKAVPAVKQTPLFDEDLVVEGILSDLEEL 520

Query: 421 PTTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQ 480
           P +EL+ PLF+SLLGLGF+MAEAKLA++NNLS LFYECK YVVATCQ SSW+NKVDDICQ
Sbjct: 521 PPSELFEPLFISLLGLGFIMAEAKLAKNNNLSSLFYECKGYVVATCQRSSWSNKVDDICQ 580

Query: 481 VYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDEQTKKSSARNANS 540
           VYETVETMMVNP+EILKAMKQ EESNMT   LKRRFKK+SLN VGKD  ++KSS +N NS
Sbjct: 581 VYETVETMMVNPEEILKAMKQPEESNMTGGELKRRFKKMSLNFVGKDGDSRKSSPKNVNS 640

Query: 541 DERLSSSTQTFSSFFDSKSSLLAKKPPKPESTS-ATPVENGWTLV 584
           DER SS   +FSSFFDSKSSL AKKPPKPE+ S ATP ENGWT V
Sbjct: 641 DERPSS--PSFSSFFDSKSSLFAKKPPKPETLSAATPDENGWTFV 683

BLAST of Carg25355 vs. TAIR 10
Match: AT5G55060.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1); Has 223 Blast hits to 218 proteins in 80 species: Archae - 0; Bacteria - 2; Metazoa - 117; Fungi - 6; Plants - 65; Viruses - 0; Other Eukaryotes - 33 (source: NCBI BLink). )

HSP 1 Score: 645.6 bits (1664), Expect = 3.9e-185
Identity = 342/575 (59.48%), Postives = 428/575 (74.43%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           M+ PFYDEN Y+L  K + EAK +++   VES  A D  SIP  SV+KQLA+AIEAGK++
Sbjct: 94  MAAPFYDENFYILKAKQEQEAKASDVGCMVESLNAVDANSIPLASVVKQLAVAIEAGKRA 153

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           K+VK  +ASS  SS  + + GL+LS V++LVL EKE+K+      ++++ SLI +LF+  
Sbjct: 154 KNVKDFVASSGSSSPVRERGGLSLSAVKSLVLGEKEDKLGFDSGDEDKLVSLINALFNVD 213

Query: 121 GDFLRRNITSGSEG-TIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDE 180
             FL R I S  E  T  +S  +D+H APP S +VK++EVIGSF + R+M LFWCR+V+E
Sbjct: 214 SGFLSRKIVSDLESPTNRSSFAKDLHAAPPSSFVVKLAEVIGSFTTPRRMALFWCRVVEE 273

Query: 181 MRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASS 240
           +RRFW+EE++IP IP+D  PDL SCLL+Q  QVINCCL+RK R+  AS++LD V+R ASS
Sbjct: 274 LRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAASEALDAVMRQASS 333

Query: 241 NTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLT 300
             E   S+V+    SLLYA+ N+GEL LRLG      NLTMLETGE VYSPITQEGPLLT
Sbjct: 334 ANEK--SDVSESMGSLLYAKSNSGELILRLGVYNQVENLTMLETGEPVYSPITQEGPLLT 393

Query: 301 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNG 360
           ED+I+ETEE VLRTGS+GAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE    NG
Sbjct: 394 EDLIRETEELVLRTGSMGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE----NG 453

Query: 361 SVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDL 420
           ++    +S  RGQLS+RMQKEGNLWRELW+T+K +PAVKQ PLFDEDL VEGIL  LED+
Sbjct: 454 NMSGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLEDI 513

Query: 421 PTTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQ 480
           P  EL+  LF SL+ LGFVM E  LA +++LSKLF+ECKDYVVA CQG +W +K+DD+CQ
Sbjct: 514 PAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFECKDYVVAICQGGAWTDKLDDLCQ 573

Query: 481 VYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDE--QTKKSSARNA 540
           VYETVETM++ P+E+L++MKQ EES  + S  KRRFK+L     G  E  QT+  S    
Sbjct: 574 VYETVETMLLRPEEVLRSMKQTEESPSSGSETKRRFKRLGFIFRGSKERKQTRVPSETEQ 633

Query: 541 NSDERLSSSTQTFSSFFDSKSSLLAKKPPKPESTS 573
            S E   S  Q+FSS FD KSSL +K+PP+PE+ +
Sbjct: 634 KSTE--PSPRQSFSSLFDGKSSLFSKRPPRPENVT 660

BLAST of Carg25355 vs. TAIR 10
Match: AT5G55060.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G58510.1). )

HSP 1 Score: 645.6 bits (1664), Expect = 3.9e-185
Identity = 342/575 (59.48%), Postives = 428/575 (74.43%), Query Frame = 0

Query: 1   MSVPFYDENLYMLNMKNDIEAKNAEIIPSVESFLATDKASIPSLSVIKQLAIAIEAGKKS 60
           M+ PFYDEN Y+L  K + EAK +++   VES  A D  SIP  SV+KQLA+AIEAGK++
Sbjct: 1   MAAPFYDENFYILKAKQEQEAKASDVGCMVESLNAVDANSIPLASVVKQLAVAIEAGKRA 60

Query: 61  KSVKSLLASSAGSSSGKGKPGLNLSTVRALVLREKEEKISTTFRHDERIQSLIRSLFDAG 120
           K+VK  +ASS  SS  + + GL+LS V++LVL EKE+K+      ++++ SLI +LF+  
Sbjct: 61  KNVKDFVASSGSSSPVRERGGLSLSAVKSLVLGEKEDKLGFDSGDEDKLVSLINALFNVD 120

Query: 121 GDFLRRNITSGSEG-TIAASLPRDIHGAPPDSLLVKISEVIGSFRSLRKMTLFWCRIVDE 180
             FL R I S  E  T  +S  +D+H APP S +VK++EVIGSF + R+M LFWCR+V+E
Sbjct: 121 SGFLSRKIVSDLESPTNRSSFAKDLHAAPPSSFVVKLAEVIGSFTTPRRMALFWCRVVEE 180

Query: 181 MRRFWSEEQYIPGIPIDEIPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASS 240
           +RRFW+EE++IP IP+D  PDL SCLL+Q  QVINCCL+RK R+  AS++LD V+R ASS
Sbjct: 181 LRRFWNEEKHIPWIPLDNNPDLKSCLLHQWLQVINCCLARKARNVAASEALDAVMRQASS 240

Query: 241 NTESGTSEVTVPANSLLYARLNNGELALRLGADCPFSNLTMLETGEKVYSPITQEGPLLT 300
             E   S+V+    SLLYA+ N+GEL LRLG      NLTMLETGE VYSPITQEGPLLT
Sbjct: 241 ANEK--SDVSESMGSLLYAKSNSGELILRLGVYNQVENLTMLETGEPVYSPITQEGPLLT 300

Query: 301 EDVIKETEEFVLRTGSVGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTEPEPSNG 360
           ED+I+ETEE VLRTGS+GAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE    NG
Sbjct: 301 EDLIRETEELVLRTGSMGAGCSQLLSDMQAFKAANPGCILEDFVRWHSPPDWTE----NG 360

Query: 361 SVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKAVPAVKQTPLFDEDLVVEGILTDLEDL 420
           ++    +S  RGQLS+RMQKEGNLWRELW+T+K +PAVKQ PLFDEDL VEGIL  LED+
Sbjct: 361 NMSGDDSSPVRGQLSTRMQKEGNLWRELWETAKPLPAVKQAPLFDEDLAVEGILNYLEDI 420

Query: 421 PTTELYGPLFLSLLGLGFVMAEAKLAEDNNLSKLFYECKDYVVATCQGSSWNNKVDDICQ 480
           P  EL+  LF SL+ LGFVM E  LA +++LSKLF+ECKDYVVA CQG +W +K+DD+CQ
Sbjct: 421 PAAELFEQLFTSLVSLGFVMVEPVLATNDDLSKLFFECKDYVVAICQGGAWTDKLDDLCQ 480

Query: 481 VYETVETMMVNPDEILKAMKQAEESNMTTSVLKRRFKKLSLNLVGKDE--QTKKSSARNA 540
           VYETVETM++ P+E+L++MKQ EES  + S  KRRFK+L     G  E  QT+  S    
Sbjct: 481 VYETVETMLLRPEEVLRSMKQTEESPSSGSETKRRFKRLGFIFRGSKERKQTRVPSETEQ 540

Query: 541 NSDERLSSSTQTFSSFFDSKSSLLAKKPPKPESTS 573
            S E   S  Q+FSS FD KSSL +K+PP+PE+ +
Sbjct: 541 KSTE--PSPRQSFSSLFDGKSSLFSKRPPRPENVT 567

BLAST of Carg25355 vs. TAIR 10
Match: AT5G58510.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G55060.2); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 139.0 bits (349), Expect = 1.2e-32
Identity = 129/478 (26.99%), Postives = 211/478 (44.14%), Query Frame = 0

Query: 140 LPRDIHGAPPDSLLVK--ISEVIGSFRSLRKMTLFWCRIVDEMRRFWSEEQYIPGIPIDE 199
           L R +  AP +SL  +  +  +     ++R +   W   V E+R  W E Q +P +PID 
Sbjct: 411 LSRALKAAPLESLFTQFCLHSLWFGNCNIRAIAFLWIEFVREVRWCWEETQPLPKMPIDG 470

Query: 200 IPDLNSCLLYQRFQVINCCLSRKRRHEIASDSLDVVLRVASS----------------NT 259
             DL+SCL+ Q+  ++  C+ +KR  E+  + LD +    SS                NT
Sbjct: 471 SIDLSSCLINQKLHLLAICIEKKR--EMNEEFLDCIGSDDSSDASVSMEEHHKVDKRRNT 530

Query: 260 ES--------GTSEVTVPANSLLYAR-------LNNGEL-ALRLGADCPFSNLTMLETGE 319
            S         +S     +  L + R       +N     A+R G+  P   + +L++ +
Sbjct: 531 SSEEELRRKRDSSIAEDTSKQLRFERKTERTNSVNQSPTDAIRRGSAGPVGTMMLLKSRQ 590

Query: 320 KVYSPITQEGPLLTEDVIKETEEFVLRTGSVGAGCSQ-----LLSDMQAFKAANPGCILE 379
           ++++P TQ+ PL+TED+ +E  + V   G       Q     LLSDM AFKAANP  + E
Sbjct: 591 QLHAPFTQDPPLMTEDMHEERLQAVEAFGDSLNVPGQLEKDILLSDMSAFKAANPDAVFE 650

Query: 380 DFVRWHSPPDW-------TEPEPSNGSVHSSIASDSRGQLSSRMQKEGNLWRELWDTSKA 439
           DF+RWHSP DW       TEP     +  S      RG+LS RM  +GNLWR+ W+ + A
Sbjct: 651 DFIRWHSPGDWESFEPKTTEPSAGPSTEGSKDEWPPRGRLSQRMSDQGNLWRKSWNDAPA 710

Query: 440 VPAVKQTPLFDEDLVVEGILTDLEDLPTTELYGPLFLSLL-GLGFVMAEAKLAEDNNL-S 499
           +PA  Q PL D +   E I+  LE +   +L   +  +   G    + +  +     + S
Sbjct: 711 LPADDQKPLLDPNREGEKIVHYLETVRPHQLLEQMVCTAFRGSADTLNQTNVGNMRQMTS 770

Query: 500 KL--FYECKDYVVATCQGSSWNNKVDDI------CQVYETVETMMVNPDEILKAMKQAEE 559
           KL   Y    + +   Q ++  +K   +      C V+E VE ++     I +  K  + 
Sbjct: 771 KLEQLYLIMKFTLGALQRNNLPDKAKTVKDLKRLCMVFENVEKLVAVAASIHR--KFLDA 830