Carg25339 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25339
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioninactive poly [ADP-ribose] polymerase RCD1
LocationCarg_Chr19: 1540965 .. 1545072 (+)
RNA-Seq ExpressionCarg25339
SyntenyCarg25339
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATATGGAAACAGAGTTAACTAGGGTTCAGCATAATGGCCAAAAGATTACTGTGGGTTTGAAGAGGAAAAGGGCAAATCAAGGCACGACACATGTTGGAGCTGGCTACGAAGCATTTTTGTCCAAGTTGACCTCGTCTAGGAATAGACATGCCAAGTGCAAGAGACTTGATGACTGTGAAGTCAGTCGTGGGCCAAACTCAAGGAGATCTTTTCTTAGAAACTATAAAAATTTTAGCAGAAGTGGACTTCCCAAGAGGTTTTTATATCACGAAGATGGGGATTGGGTAGATTACCCTCAGATAACTGTGAAGTTGATTAGGGAACAGTTTCAGATTAAAAATGCGGCAATTGAAGTGGAATTGAATGGCCGCCATTTGCTGCTAGATGCTTTGTATATGATCCAAGTGGAACTGAAAACAGCTATTCAGCAGCACATTGCCTGGATTGATGACAATGGTCAATGTTTCTTTCCAGAATTTTATTCGGGTGACAGTGTGATGCATCCGTTCCACCAGCATGAGCTGGAAGATGCTTCTACTGTGGCATTACCAGACCATACAGGAATGAGAGAGATCAATTTGCACCTTGAAATTGGAATAACTGAAGCAAATAGTTCTCAATCAGAAGGCTATATTGAAGAGTCAAATAGAGATGCTAAGATATCTAATATCAAAGGAAAAACTTTTGATATTGCTGAGGATTGGGAAGACAGTGAAACTAATAGTCAGAAACCAGTTGCTCAAATGCAGGAGGCTGTTGGAGGAATAAAAGAGATTGATTGTTATGTGACGCCTCAAAATGAAATAATTCATTCAACTGTGGGTTTGGATGATGTAAAAAATATGTTCTTAATGAGCATGGAATCTATTAAGAACATTGAAATACTTGAGGTCAAACGCTGGTCTAGTCATTTAATGCAGGATAGACTGGATCTGTTTAAGAAACAAATAGAGATAACCAAGAAGAGTCGTGGGAATGCCAATGTTCAGTATGGTTGGCTAGCTGGTAGCATGCTACATGGGGTAGGTCATTTTGAGCCGAAGCTTACATCCACATCTGGTGTTCACCTTTCAAGCTGCGCAAATCTTAGGTATGCATTTCATCTTCCTTCAGTTAAATAGTATATGGAAATTAATGGCGGTTTATTGTATGATGAGATAATAGGCTAATAGTTACCATATTATAATATATGTTTTCATGTTTTTATGAGCTTTATGGACCTCTCAACCCTTGCAACAAATACTAATGCTGGTTGTGATTTTCTGGTGTTTAAAAACCCACGCCTCATGCTAAAGAAAATCACCTCCTCTTCCATTCTCTTTTTGCACCATATGATCCTGACAAAGAATCACATTACGGTCATGGCAATACTGAAACAGTATATAAACAACTGCAGTATGTTATTTTTAGCTTTAGCTGTATGGATAACTCTTTTGTTCTTTATTATTCCTGGAGATTAACTTCTGCACTGGTTTAGTGCAAGCTACTGCGACGATGATGAAAAGGGGGTTAGATACATAGCATTCTGTCATGTCATATTGGGAAATGTGGAAGTTGTCACTCCTGGATGTGTACCATATCATCCCAGTTCTGTAAACTTTGACAGTGGAGTAGATGACCTTCATAATCCAAGTCAATATTTTGTTTGGAATATGAATATGAAAAGCCACATTTTCCAGGAGTATGTTGTCAGTTTCAAGATATCATCTAGCCCTCCTGAATGTGGTAAGTTCTCTCTTGGTTGCTATTGCTCATTTCTTGTTTATGGGATCATAACGTTTTTTTGAATCTTGCATGGCGCATGTATCTTGTAGAATAGTTGGAACTGGTCAGGATTCATGAATGATGTGATGTTTTTTATTATTTAAATATTTATTTATTTTCCCTCACTCTCTCCCCGCCTTTTGTGTGTGTGTGGGGGTGGGGGGGACTTGTGGAGATCTAAGTTGGTATTCTGATCCGTTTTCTTGGAATTGATTCTGAAGGGCTCCAGGACCAGTTGCAGTTAAATTGCTCTTCGATTGAATCAGTAAGCCATCTTTTATGGGCTATAATATTATGTTCTATGTGGGATTTTTCCAGTGAATTCTTGTCTGTGGTTTCGTTGCCACTCTTGTTAGGTGGACTTGTGAACCTATGAGTTATAATTATGAAAGCTGTTATTTATTCAATATGAGTTGAGCTTAATTTTGGGTCAGCATAGATTTCCTAAGGTTGCCTTCCATCTCGGCCTGCCAAAGCCTTTATTCCCATGTATAGCTGAGCTAATTTGTTTTCGTTTCCTCCTTGACCTGACCACCTTTATAATTGTTTGCATTCACAATTCTTGGGTTTATGACTTTCTGGAATCAGCTTTCTTTCCTACTTACCTACTTCAATAGTTGGGTGATCTGCCTTTGAAGATTCATTAACCGCAACATTCTAACTCCTAGGTGCAGGCTTCACTTGCCAAATTACTTTTTCCTTTGATTATCGTACATTGCTCTGTAACTATTCAATTGGCACATTACATAGCATCCTGGGCAAACCAGATTACTGGGGTTCTTTTTAGGAAGACCAAGGAAACAATCTTGTTTCATGGATGCCCTTATGGTTAATACTGTGAGAGCATACTCTTTTTCTGACCTTTGGATGCTGTTGGGTTTTCGATGGGACAGTTCATAAACTTGGAGCCTTTATGCGATCAGGTTTTAGAAGAAATGCAAAAGTTCTTTTAGTTTATGAAACAAAAGCTTTAGGGAGTATGGACGGGGATGAATCAAATAACTTTTAAGGGGAAGGATGGGAATTGGGAAGAAAAATTTTATTTGCGAAGCGTCATTGTTATTGTGATTCTCACCCCTTTTTCTGCAAATTGCCTAATAGAGGTTTATGCTGTTTTTGCAAACATGATAATTTTCTTCTGGATCAATATGAAGTGTTTTTTTCCACCATAAAGAAGAGAGTTTTGCGAAAGAGTTCAAAGAAATCGTCTTATACCACAACATTAATAGAAAAGAAAGTTTGGGATGCTCAAGTTATTAAAGCTAGATTTCTTTTTCATTTCATCTTAATATTAAGGGAAGCCATTTACTTTTAGGTGGAGGATTGTGTGTCTTCGCCAGTTCCTGAAAAGAGGTGCACAGAAAAGCATGACCATGGATCTAGCTCCCTGAAGACTCCGAAGTCTCCCTGGATGCCTTTTTCAATGTTGTTTAATGCCATTTCAAAAGAAGTCTCTCTTAACGACATGAAATTGGTGAAGGGTCATTATTGCTTATTTAGAGTAAGTGAATGTGTCTATAAGTGCTTTCCTGTTATTTGATTTGTTTAGTGGATAATCTTGATATTCCTTAATATATTAGGAAAAGAAGATCAGTAGGGATGAATTCATAAGACGGTTGAGGTCCATTGTTGGGGATCCGTTACTGAGATCTATGCTAATGAGTCTCCAGTGCAAGGTGTGTTTTATGTGTGATCACTATTGTTCAGTTCTTGTATGATGTAGAGCCGTTTTGTTGCTATGCGAAAGACTGGCATTTGGATATGGATCACGTGCAGCTGTCAACATTATAATTTACAATTTTCTTTTCCGGGTGAAATGCTATTTGTTTGATTAAAAGGATTGGAAATATACCAAGTATGATCTTTGCAAACAGAATAAAATATTATGACTGTTATCAATCGGGCCACTACATGTTGGTTGTTAAATATAAACATGTTTTTCGTTTTTAACCTTGAATTAAAGTGTACTGTACTCTCCTTTCCTGACCAAGTAACATTTCTATATGGATCTTAGCATGGACGATGGTAGCTGTTTCTGTTACATGCAACTTGTTAAGTAGTCTTTTCTTGTTTCAGCTACCTCCGAAGTCTCCATGTGCTTTCAAAGTTCCTAAGGAGGAGAAAGAATGTTGAGGCTGTTGGTAAGAGTCTGTATTTAGGTGCTTTTCGTCATGGGGGTTACTAATGTACATGGCGTCTTACTGTTATTTTGCCTTGGAATTTGTCGATCCGATTTGTGACATCACGACATAAGCTTTGTGAGATGGTCACTATTTCTTATGTACACTTCTCAGTTAATTGATTATTTAATACACTCAGACAATTGCGTCCATTCTTATA

mRNA sequence

ATGAATATGGAAACAGAGTTAACTAGGGTTCAGCATAATGGCCAAAAGATTACTGTGGGTTTGAAGAGGAAAAGGGCAAATCAAGGCACGACACATGTTGGAGCTGGCTACGAAGCATTTTTGTCCAAGTTGACCTCGTCTAGGAATAGACATGCCAAGTGCAAGAGACTTGATGACTGTGAAGTCAGTCGTGGGCCAAACTCAAGGAGATCTTTTCTTAGAAACTATAAAAATTTTAGCAGAAGTGGACTTCCCAAGAGGTTTTTATATCACGAAGATGGGGATTGGGTAGATTACCCTCAGATAACTGTGAAGTTGATTAGGGAACAGTTTCAGATTAAAAATGCGGCAATTGAAGTGGAATTGAATGGCCGCCATTTGCTGCTAGATGCTTTGTATATGATCCAAGTGGAACTGAAAACAGCTATTCAGCAGCACATTGCCTGGATTGATGACAATGGTCAATGTTTCTTTCCAGAATTTTATTCGGGTGACAGTGTGATGCATCCGTTCCACCAGCATGAGCTGGAAGATGCTTCTACTGTGGCATTACCAGACCATACAGGAATGAGAGAGATCAATTTGCACCTTGAAATTGGAATAACTGAAGCAAATAGTTCTCAATCAGAAGGCTATATTGAAGAGTCAAATAGAGATGCTAAGATATCTAATATCAAAGGAAAAACTTTTGATATTGCTGAGGATTGGGAAGACAGTGAAACTAATAGTCAGAAACCAGTTGCTCAAATGCAGGAGGCTGTTGGAGGAATAAAAGAGATTGATTGTTATGTGACGCCTCAAAATGAAATAATTCATTCAACTGTGGGTTTGGATGATGTAAAAAATATGTTCTTAATGAGCATGGAATCTATTAAGAACATTGAAATACTTGAGGTCAAACGCTGGTCTAGTCATTTAATGCAGGATAGACTGGATCTGTTTAAGAAACAAATAGAGATAACCAAGAAGAGTCGTGGGAATGCCAATGTTCAGTATGGTTGGCTAGCTGGTAGCATGCTACATGGGGTAGGTCATTTTGAGCCGAAGCTTACATCCACATCTGGTGTTCACCTTTCAAGCTGCGCAAATCTTAGGTATGCATTTCATCTTCCTTCAGTTAAATATGCAAGCTACTGCGACGATGATGAAAAGGGGGTTAGATACATAGCATTCTGTCATGTCATATTGGGAAATGTGGAAGTTGTCACTCCTGGATGTGTACCATATCATCCCAGTTCTGTAAACTTTGACAGTGGAGTAGATGACCTTCATAATCCAAGTCAATATTTTGTTTGGAATATGAATATGAAAAGCCACATTTTCCAGGAGTATGTTGTCAGTTTCAAGATATCATCTAGCCCTCCTGAATGTGGGCTCCAGGACCAGTTGCAGTTAAATTGCTCTTCGATTGAATCAGTGGAGGATTGTGTGTCTTCGCCAGTTCCTGAAAAGAGGTGCACAGAAAAGCATGACCATGGATCTAGCTCCCTGAAGACTCCGAAGTCTCCCTGGATGCCTTTTTCAATGTTGTTTAATGCCATTTCAAAAGAAGTCTCTCTTAACGACATGAAATTGGTGAAGGGTCATTATTGCTTATTTAGAGAAAAGAAGATCAGTAGGGATGAATTCATAAGACGGTTGAGGTCCATTGTTGGGGATCCGTTACTGAGATCTATGCTAATGAGTCTCCAGTGCAAGCTACCTCCGAAGTCTCCATGTGCTTTCAAAGTTCCTAAGGAGGAGAAAGAATGTTGAGGCTGTTGGTAAGAGTCTGTATTTAGGTGCTTTTCGTCATGGGGGTTACTAATGTACATGGCGTCTTACTGTTATTTTGCCTTGGAATTTGTCGATCCGATTTGTGACATCACGACATAAGCTTTGTGAGATGGTCACTATTTCTTATGTACACTTCTCAGTTAATTGATTATTTAATACACTCAGACAATTGCGTCCATTCTTATA

Coding sequence (CDS)

ATGAATATGGAAACAGAGTTAACTAGGGTTCAGCATAATGGCCAAAAGATTACTGTGGGTTTGAAGAGGAAAAGGGCAAATCAAGGCACGACACATGTTGGAGCTGGCTACGAAGCATTTTTGTCCAAGTTGACCTCGTCTAGGAATAGACATGCCAAGTGCAAGAGACTTGATGACTGTGAAGTCAGTCGTGGGCCAAACTCAAGGAGATCTTTTCTTAGAAACTATAAAAATTTTAGCAGAAGTGGACTTCCCAAGAGGTTTTTATATCACGAAGATGGGGATTGGGTAGATTACCCTCAGATAACTGTGAAGTTGATTAGGGAACAGTTTCAGATTAAAAATGCGGCAATTGAAGTGGAATTGAATGGCCGCCATTTGCTGCTAGATGCTTTGTATATGATCCAAGTGGAACTGAAAACAGCTATTCAGCAGCACATTGCCTGGATTGATGACAATGGTCAATGTTTCTTTCCAGAATTTTATTCGGGTGACAGTGTGATGCATCCGTTCCACCAGCATGAGCTGGAAGATGCTTCTACTGTGGCATTACCAGACCATACAGGAATGAGAGAGATCAATTTGCACCTTGAAATTGGAATAACTGAAGCAAATAGTTCTCAATCAGAAGGCTATATTGAAGAGTCAAATAGAGATGCTAAGATATCTAATATCAAAGGAAAAACTTTTGATATTGCTGAGGATTGGGAAGACAGTGAAACTAATAGTCAGAAACCAGTTGCTCAAATGCAGGAGGCTGTTGGAGGAATAAAAGAGATTGATTGTTATGTGACGCCTCAAAATGAAATAATTCATTCAACTGTGGGTTTGGATGATGTAAAAAATATGTTCTTAATGAGCATGGAATCTATTAAGAACATTGAAATACTTGAGGTCAAACGCTGGTCTAGTCATTTAATGCAGGATAGACTGGATCTGTTTAAGAAACAAATAGAGATAACCAAGAAGAGTCGTGGGAATGCCAATGTTCAGTATGGTTGGCTAGCTGGTAGCATGCTACATGGGGTAGGTCATTTTGAGCCGAAGCTTACATCCACATCTGGTGTTCACCTTTCAAGCTGCGCAAATCTTAGGTATGCATTTCATCTTCCTTCAGTTAAATATGCAAGCTACTGCGACGATGATGAAAAGGGGGTTAGATACATAGCATTCTGTCATGTCATATTGGGAAATGTGGAAGTTGTCACTCCTGGATGTGTACCATATCATCCCAGTTCTGTAAACTTTGACAGTGGAGTAGATGACCTTCATAATCCAAGTCAATATTTTGTTTGGAATATGAATATGAAAAGCCACATTTTCCAGGAGTATGTTGTCAGTTTCAAGATATCATCTAGCCCTCCTGAATGTGGGCTCCAGGACCAGTTGCAGTTAAATTGCTCTTCGATTGAATCAGTGGAGGATTGTGTGTCTTCGCCAGTTCCTGAAAAGAGGTGCACAGAAAAGCATGACCATGGATCTAGCTCCCTGAAGACTCCGAAGTCTCCCTGGATGCCTTTTTCAATGTTGTTTAATGCCATTTCAAAAGAAGTCTCTCTTAACGACATGAAATTGGTGAAGGGTCATTATTGCTTATTTAGAGAAAAGAAGATCAGTAGGGATGAATTCATAAGACGGTTGAGGTCCATTGTTGGGGATCCGTTACTGAGATCTATGCTAATGAGTCTCCAGTGCAAGCTACCTCCGAAGTCTCCATGTGCTTTCAAAGTTCCTAAGGAGGAGAAAGAATGTTGA

Protein sequence

MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDCEVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDASTVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC
Homology
BLAST of Carg25339 vs. NCBI nr
Match: KAG7011369.1 (Inactive poly [ADP-ribose] polymerase RCD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1191.4 bits (3081), Expect = 0.0e+00
Identity = 584/584 (100.00%), Postives = 584/584 (100.00%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK
Sbjct: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS
Sbjct: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 584

BLAST of Carg25339 vs. NCBI nr
Match: XP_023553913.1 (inactive poly [ADP-ribose] polymerase RCD1-like [Cucurbita pepo subsp. pepo] >XP_023553914.1 inactive poly [ADP-ribose] polymerase RCD1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1147.1 bits (2966), Expect = 0.0e+00
Identity = 567/584 (97.09%), Postives = 570/584 (97.60%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKT DIAEDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTLDIAEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK
Sbjct: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLT+TSGVHLSS
Sbjct: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTATSGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYC+DDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCNDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSML SLQCKLPPKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLTSLQCKLPPKSPCAFKVPKEEKEC 574

BLAST of Carg25339 vs. NCBI nr
Match: XP_022963963.1 (inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita moschata] >XP_022963964.1 inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 564/584 (96.58%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGT HVGAGY+AFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTAHVGAGYKAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIK KTFDIAEDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKAKTFDIAEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIK+IEILEVK
Sbjct: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKDIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDR DLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS
Sbjct: 301 RWSSHLMQDRPDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSML SLQCKL PKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLTSLQCKLLPKSPCAFKVPKEEKEC 574

BLAST of Carg25339 vs. NCBI nr
Match: KAG6571640.1 (Inactive poly [ADP-ribose] polymerase RCD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 561/579 (96.89%), Postives = 565/579 (97.58%), Query Frame = 0

Query: 6   ELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDCEVSRG 65
           +LTRVQHNGQKITVGLKRKRANQGT HVGAGYEAFLSKLTSSRNRHAKCKRLDDCEVSRG
Sbjct: 36  KLTRVQHNGQKITVGLKRKRANQGTAHVGAGYEAFLSKLTSSRNRHAKCKRLDDCEVSRG 95

Query: 66  PNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEVELNGR 125
           PNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEVELNGR
Sbjct: 96  PNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEVELNGR 155

Query: 126 HLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDASTVALP 185
           HLLLDALYMIQVELKTAIQQHIAWIDD+GQCFFPEFYSGDSVMHPFHQ+ELEDASTVALP
Sbjct: 156 HLLLDALYMIQVELKTAIQQHIAWIDDSGQCFFPEFYSGDSVMHPFHQYELEDASTVALP 215

Query: 186 DHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSETNSQK 245
           DHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIK KTFDIAEDWEDSETNSQK
Sbjct: 216 DHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKAKTFDIAEDWEDSETNSQK 275

Query: 246 PVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVKRWSSH 305
           PVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIK+IEILEVKRWSSH
Sbjct: 276 PVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKDIEILEVKRWSSH 335

Query: 306 LMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSSCANLR 365
           LMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSSCANL 
Sbjct: 336 LMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSSCANLS 395

Query: 366 YAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHN 425
                     ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHN
Sbjct: 396 ----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHN 455

Query: 426 PSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKR 485
           PSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKR
Sbjct: 456 PSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKR 515

Query: 486 CTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIR 545
           CTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIR
Sbjct: 516 CTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIR 575

Query: 546 RLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           RLRSIVGDPLLRSML SLQCKLPPKSPCAFKVPKEEKEC
Sbjct: 576 RLRSIVGDPLLRSMLTSLQCKLPPKSPCAFKVPKEEKEC 604

BLAST of Carg25339 vs. NCBI nr
Match: XP_022967219.1 (inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita maxima] >XP_022967220.1 inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 559/584 (95.72%), Postives = 562/584 (96.23%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLY EDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYQEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMH FHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHRFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGI EANSSQSEGYIEESNR  KISNIK KTFD+ EDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGIAEANSSQSEGYIEESNRHTKISNIKAKTFDVGEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQM EAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK
Sbjct: 241 TNSQKPVAQMHEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTST+GVHLSS
Sbjct: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTAGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMK VKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKSVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSML SLQCKLPPKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLTSLQCKLPPKSPCAFKVPKEEKEC 574

BLAST of Carg25339 vs. ExPASy Swiss-Prot
Match: Q8RY59 (Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RCD1 PE=1 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 3.8e-76
Identity = 203/600 (33.83%), Postives = 317/600 (52.83%), Query Frame = 0

Query: 3   METELTRVQHNGQ-KITVGLKRKRANQGTTHVGAGYEAFL-----SKLTSSRNRHAKCKR 62
           ME ++ +V  + + +   G KRKRA        A Y A++     +KL +    + +C+ 
Sbjct: 1   MEAKIVKVLDSSRCEDGFGKKRKRA--------ASYAAYVTGVSCAKLQNVPPPNGQCQI 60

Query: 63  LDDCEVSRGPN--------SRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIR 122
            D      G N        S ++ +R Y  F ++G+ KR + +E+G+W D P+  +  I+
Sbjct: 61  PDKRRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQ 120

Query: 123 EQFQIKNAAIEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVM 182
            + + K+AAIE +L G   +LD L+M +++++T  +  +AWID+ G+CFFPE Y  D   
Sbjct: 121 NELEEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERT 180

Query: 183 HPFHQHELEDASTVALPDHTGMREINLHLEIGIT--EANSSQSEGYIEESNRDAKISNIK 242
           +  H   +ED      P      +I L LEI +   E      E   +ES  D  + ++ 
Sbjct: 181 NYCHHKCVED------PKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESG-DNMMDDVP 240

Query: 243 GKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTV-GLDDVKNMFL 302
                  E ++++  +S     +++ AV    E D  V    ++  S V   D VK MF 
Sbjct: 241 LAQRSSNEHYDEATEDSCS--RKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFA 300

Query: 303 MSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW-------LAGS 362
           +   S+ ++ +L+V R+SS + + RL LF+KQ+EITKK RG+ANV+Y W       L+  
Sbjct: 301 VGTASLGHVPVLDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAV 360

Query: 363 MLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVI 422
           M+ G+   G F  K     G+HL++ A+  Y         A YCD DE GVRY+  C VI
Sbjct: 361 MMQGLGVGGAFIRKSIYGVGIHLTA-ADCPYF-------SARYCDVDENGVRYMVLCRVI 420

Query: 423 LGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPP 482
           +GN+E++      +      +D+GVDD+ +P  Y VWN+NM +HIF E+VV FK+S+ P 
Sbjct: 421 MGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSNLPN 480

Query: 483 ECG----LQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGSSSLKTPKSPWMPFSMLF 542
             G     +D   +     + +   + S    +     +  GSS+ + PKSPWMPF  LF
Sbjct: 481 AEGNLIAKRDNSGVTLEGPKDLPPQLESNQGARGSGSANSVGSSTTR-PKSPWMPFPTLF 540

Query: 543 NAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPPKS 572
            AIS +V+ NDM L+   Y   R+KK++R EF+R+LR IVGD LLRS + +LQ +  PKS
Sbjct: 541 AAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQ--PKS 572

BLAST of Carg25339 vs. ExPASy Swiss-Prot
Match: O82289 (Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3702 GN=SRO1 PE=1 SV=2)

HSP 1 Score: 274.6 bits (701), Expect = 2.5e-72
Identity = 201/606 (33.17%), Postives = 304/606 (50.17%), Query Frame = 0

Query: 3   METELTRVQHNGQKITVGLKRKRANQGTTH-VGAGYEAFLSKLT--SSRNRHAKCKRLD- 62
           ME ++ +V  +  K  +G KRK     T +  G  Y      L+  SS  +  K + LD 
Sbjct: 1   MEAKIVKVSDSSYKDGLGKKRKHPGNYTPYDSGRSYAKLQWVLSPNSSTQKLEKRRNLDG 60

Query: 63  DCEVSRGPNS-RRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAA 122
           + +V    N   +S +R +  + ++G+PKR ++HE+G+W+D P   +  IR   + K A 
Sbjct: 61  ENKVIVSENHVEKSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRAT 120

Query: 123 IEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHP-FHQHEL 182
           IE    GRH LLD L+M +++L+T ++  +AWID  G+CFFPE +  D++     H    
Sbjct: 121 IEFNWCGRHFLLDFLHMYRLDLETGVKTQLAWIDIAGKCFFPETF--DTLERDGCHHIRG 180

Query: 183 EDASTVALPDHTGMREINLHLEIG-------------ITEANSSQSEGY--IEES----N 242
           ED      P+    REI LH+EI              +T+ +    + +  ++ S    N
Sbjct: 181 ED------PEQHDQREIKLHIEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPN 240

Query: 243 RDAKISNIKGKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGL 302
            +A   +   +  D  E W+ +ET+    V   +E      E+D                
Sbjct: 241 DEASEDSCSRELDDAVEKWDKTETDRFSGVKPAEE------ELD---------------K 300

Query: 303 DDVKNMFLMSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW--- 362
           D VK MF +   ++ ++E L+V ++SS + + RL LF+KQ +ITKK RG+AN++Y W   
Sbjct: 301 DAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQADITKKHRGDANIRYAWVPA 360

Query: 363 ----LAGSMLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVR 422
               L+  M+HG+   G F  K     GVH ++C              A YCD D+ GVR
Sbjct: 361 KKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAANCPYFS----------ARYCDIDDNGVR 420

Query: 423 YIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVS 482
           ++  C VI+GN+E +      Y      +D+GVDD+ +P  Y +WNMNM +HI+ E+VVS
Sbjct: 421 HMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMNMNTHIYPEFVVS 480

Query: 483 FKISSSPPECGLQDQLQ-LNCSSIESVEDCVSSPVPEKRCTEKHDHG----SSSLKTPKS 542
           FK+S    E  +    Q  + SS  ++E    SP  E         G    SSS + P+S
Sbjct: 481 FKLSIPNAEGNILPTTQSRHESSGLTLEGPKGSPSNEPGRVSNGGSGSEKNSSSSRRPRS 540

Query: 543 PWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVG-DPLLRSMLM 568
           P MPF +LF AIS +++  DM L+   Y   REKK+SR EF + L  IVG D LL S + 
Sbjct: 541 PIMPFPLLFKAISSKIARKDMDLIIAGYQELREKKVSRKEFYKTLSMIVGDDDLLISTIT 567

BLAST of Carg25339 vs. ExPASy Swiss-Prot
Match: Q9ZUD9 (Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3702 GN=SRO2 PE=1 SV=1)

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-29
Identity = 87/256 (33.98%), Postives = 126/256 (49.22%), Query Frame = 0

Query: 320 ITKKSRGNANVQYGWLAGS-------MLHG-----VGHFEPKLTSTS-GVHL--SSCANL 379
           + +K  G+ANV+YGW AGS       + +G     VG FE    S   G+HL  S C   
Sbjct: 103 VARKHGGDANVKYGWYAGSRDEIQRIISYGFSNRDVGKFENDGGSHGIGIHLVPSKC--- 162

Query: 380 RYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLH 439
                  S+  AS  + DE+G+RY+  C VILG  E++  G    +PSS  FDSGVDDLH
Sbjct: 163 -------SLLAASATEQDEEGLRYLLLCRVILGKPEIIISGSKQSYPSSAEFDSGVDDLH 222

Query: 440 NPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEK 499
           NP  Y +W+ NM S I   Y+VSF+   SP       +L+++     S            
Sbjct: 223 NPRNYVIWSCNMNSCILPSYIVSFR---SP-------RLRVSRGGFAS------------ 282

Query: 500 RCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFI 559
                          P SPW+ F+ L + +S  +  + M L+   Y  FR++KI RD+ +
Sbjct: 283 --------------RPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFRKRKIRRDQLV 312

Query: 560 RRLRSIVGDPLLRSML 561
           R++R + GD LL  ++
Sbjct: 343 RKMREVAGDNLLAEII 312

BLAST of Carg25339 vs. ExPASy Swiss-Prot
Match: O64592 (Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3702 GN=SRO3 PE=1 SV=2)

HSP 1 Score: 115.2 bits (287), Expect = 2.6e-24
Identity = 88/294 (29.93%), Postives = 138/294 (46.94%), Query Frame = 0

Query: 280 VKNMFLMSMESI-KNIEILEVKRWSSHLM---QDRLDLFKKQIEITK-KSRGNANVQYGW 339
           +KN FL  M S      I+ V++  +  +   + +  +FK   E  K K+ G AN++YGW
Sbjct: 50  IKNCFLSGMGSFATETTIVTVRKILTQRLITTKAKFAVFKLFTEAMKRKNNGYANIRYGW 109

Query: 340 LAGS-------MLHGVGHFE-PKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGV 399
            +GS       + +G  + E  K+ +  G H      +    H  S+  A   + DE+G+
Sbjct: 110 YSGSKEEIDRVITYGFSNREIKKVENDVGSH---GVGIHLVHHRYSLAAALVGEGDEEGI 169

Query: 400 RYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVV 459
           + I  C VILG  E +  G    +PSS  FDSGVD+L NP +Y +W+ NM S+I   Y+V
Sbjct: 170 KNILLCRVILGKPEQIVTGSKQSYPSSNQFDSGVDNLENPRKYVIWSCNMNSYILPTYIV 229

Query: 460 SFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGSSSLKTPKSPWMP 519
           SFK   S    GL                                     +   +SP + 
Sbjct: 230 SFK---SHLLRGL-------------------------------------IGRARSPCVS 289

Query: 520 FSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSML 561
           FS+L + +SK +    M L+   Y  FR++K+ R++ +R++R +VGD LL  +L
Sbjct: 290 FSVLMSILSKSLDAARMNLILTSYDDFRKRKLRREQLVRKIREVVGDNLLFKIL 300

BLAST of Carg25339 vs. ExPASy Swiss-Prot
Match: Q9STU1 (Probable inactive poly [ADP-ribose] polymerase SRO4 OS=Arabidopsis thaliana OX=3702 GN=SRO4 PE=2 SV=2)

HSP 1 Score: 106.3 bits (264), Expect = 1.2e-21
Identity = 64/192 (33.33%), Postives = 102/192 (53.12%), Query Frame = 0

Query: 376 ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMN 435
           +S C++D  G+ ++ F  +I+G  EVV      Y PSS+ FDSGVD L +P++Y +W+ +
Sbjct: 165 SSSCNED--GISFLLFSRIIMGKSEVVCSTSQSY-PSSMEFDSGVDSLTSPNKYIIWSTH 224

Query: 436 MKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGSS 495
           M +H+  E+VV                             C+ +P   KR          
Sbjct: 225 MNTHVLPEFVV-----------------------------CIKTPSILKR---------- 284

Query: 496 SLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPL 555
             K PKSPW+ F +L N+ISK ++ + ++L+  HY   ++++ISR E I+RLRSI GD L
Sbjct: 285 --KNPKSPWISFPVLINSISKFLNQSQIRLIHKHYKEHQDRRISRCELIQRLRSITGDSL 312

Query: 556 LRSMLMSLQCKL 568
           L  ++ S+  K+
Sbjct: 345 LVQIIKSVGQKV 312

BLAST of Carg25339 vs. ExPASy TrEMBL
Match: A0A6J1HJF2 (inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111464106 PE=4 SV=1)

HSP 1 Score: 1138.3 bits (2943), Expect = 0.0e+00
Identity = 564/584 (96.58%), Postives = 567/584 (97.09%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGT HVGAGY+AFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTAHVGAGYKAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIK KTFDIAEDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKAKTFDIAEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIK+IEILEVK
Sbjct: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKDIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDR DLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS
Sbjct: 301 RWSSHLMQDRPDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSML SLQCKL PKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLTSLQCKLLPKSPCAFKVPKEEKEC 574

BLAST of Carg25339 vs. ExPASy TrEMBL
Match: A0A6J1HW58 (inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466819 PE=4 SV=1)

HSP 1 Score: 1130.2 bits (2922), Expect = 0.0e+00
Identity = 559/584 (95.72%), Postives = 562/584 (96.23%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLY EDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYQEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMH FHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHRFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGI EANSSQSEGYIEESNR  KISNIK KTFD+ EDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGIAEANSSQSEGYIEESNRHTKISNIKAKTFDVGEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQM EAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK
Sbjct: 241 TNSQKPVAQMHEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTST+GVHLSS
Sbjct: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTAGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSP 480

Query: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISR 540
           VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMK VKGHYCLFREKKISR
Sbjct: 481 VPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKSVKGHYCLFREKKISR 540

Query: 541 DEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
           DEFIRRLRSIVGDPLLRSML SLQCKLPPKSPCAFKVPKEEKEC
Sbjct: 541 DEFIRRLRSIVGDPLLRSMLTSLQCKLPPKSPCAFKVPKEEKEC 574

BLAST of Carg25339 vs. ExPASy TrEMBL
Match: A0A6J1E4V0 (inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111026079 PE=4 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.9e-265
Identity = 475/606 (78.38%), Postives = 509/606 (83.99%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMET+LT+V HNGQ+I VGLKRKR NQGT HVGAG +AFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETKLTKVLHNGQEIVVGLKRKRENQGTAHVGAGCKAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVS G  SRRSFLRNYKNFS+SGLP+RFLY+EDGDW+DYPQI V+LIREQFQI+NAA+EV
Sbjct: 61  EVSSG-TSRRSFLRNYKNFSKSGLPQRFLYYEDGDWIDYPQIIVQLIREQFQIRNAAVEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           E++GRHLLLDALYMIQVELKT +QQHIAWIDDNGQCFFPEFYSG +VMH FH +ELED S
Sbjct: 121 EVDGRHLLLDALYMIQVELKTGVQQHIAWIDDNGQCFFPEFYSGGTVMHQFHDYELEDGS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
            VAL D TGMREINLHLEIGITEANSSQSE YIEESNRD +ISNIK ++ D   DWE +E
Sbjct: 181 AVALSDQTGMREINLHLEIGITEANSSQSEEYIEESNRDIRISNIKEESCDNGGDWEGNE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
             S+KPVA+MQEAV GIKEIDCY+TP NE+I S V  DDVKNMFLM M SIKNIE+LEVK
Sbjct: 241 NKSRKPVAEMQEAVAGIKEIDCYMTPPNELIQSNVDSDDVKNMFLMGMGSIKNIEVLEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLA-------GSMLHGVGHFEPKLTST 360
           RWSSHLMQ RLDLFKKQIEITKK+RGNANVQYGWLA       GS+L+G GH  PKLTS+
Sbjct: 301 RWSSHLMQARLDLFKKQIEITKKNRGNANVQYGWLAVNKDVLSGSILYGAGHCGPKLTSS 360

Query: 361 SGVHLS--SCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHP 420
            GVHLS  SCA++           ASYCDDDEKGVRYI FCHVILGNVEVVTPGC  YHP
Sbjct: 361 YGVHLSPTSCAHVS----------ASYCDDDEKGVRYIVFCHVILGNVEVVTPGCGLYHP 420

Query: 421 SSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPEC------------GL 480
           SSVNFD+GVDDLHNPSQY VWNMNM SHIF EYVVSFK+SSSP                L
Sbjct: 421 SSVNFDNGVDDLHNPSQYIVWNMNMNSHIFPEYVVSFKVSSSPQRGILAAKNGGIDTERL 480

Query: 481 QDQLQLNCSSIESVEDCVSSPVPEKRCTEK-HDHGSSSLKTPKSPWMPFSMLFNAISKEV 540
            DQLQLNCSSIES EDCVSSP P KRCT K  DHGSSSLKTPKSPWMPFS LFNAISKEV
Sbjct: 481 HDQLQLNCSSIESEEDCVSSPAPVKRCTGKLVDHGSSSLKTPKSPWMPFSKLFNAISKEV 540

Query: 541 SLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVP 585
           SLNDMKLV GHY LFREKKISRDEFI RLRSIVGDPLLRS L SLQCK+PPKSP  FKVP
Sbjct: 541 SLNDMKLVNGHYGLFREKKISRDEFISRLRSIVGDPLLRSTLTSLQCKVPPKSPYTFKVP 595

BLAST of Carg25339 vs. ExPASy TrEMBL
Match: A0A0A0LE89 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901000 PE=4 SV=1)

HSP 1 Score: 922.2 bits (2382), Expect = 1.1e-264
Identity = 479/594 (80.64%), Postives = 498/594 (83.84%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELT+V HNGQKI VGLKRKRANQGT+H GAGY+AFLSKL+SSRNRHAK +RLDDC
Sbjct: 38  MNMETELTKVLHNGQKIVVGLKRKRANQGTSHAGAGYKAFLSKLSSSRNRHAKSRRLDDC 97

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVS GPNSRRS LR YKNF RSGLP+RFLY+EDGDWVDYPQI VKLIREQFQIKNAAIEV
Sbjct: 98  EVSCGPNSRRSLLRTYKNFIRSGLPQRFLYYEDGDWVDYPQIIVKLIREQFQIKNAAIEV 157

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           E NGRHLLLDALYMIQVELKTAIQQHIAWIDDN QCFFPEFYSGD +MH +H +ELEDAS
Sbjct: 158 EFNGRHLLLDALYMIQVELKTAIQQHIAWIDDNDQCFFPEFYSGDRMMHQYHPYELEDAS 217

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
             AL DHTGMREINLHLEIG+TEAN SQSE Y+EESNRD K SNIK KT DI  DWE SE
Sbjct: 218 AAALSDHTGMREINLHLEIGLTEANISQSEEYVEESNRDTKKSNIKAKTCDIVGDWEGSE 277

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           T SQKPVA  QEAVG IKEIDCY TPQNE+IHS V  DDVKNMFLMSMESIKNIEILEVK
Sbjct: 278 TRSQKPVAGTQEAVGEIKEIDCYSTPQNEVIHSIVNSDDVKNMFLMSMESIKNIEILEVK 337

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLA-------GSMLH-GVGHFEPKLTS 360
           RWSS LMQDRLDLF KQIE T K+RGNANVQYGWLA       G M + GVGH   K TS
Sbjct: 338 RWSSPLMQDRLDLFHKQIETTMKNRGNANVQYGWLALDKDILSGRMPYGGVGHSGLKPTS 397

Query: 361 TSGVHL--SSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYH 420
             GV L  SSCANL           ASYCDDDEKGVRYIAFCHVILGNVEVV PGC PYH
Sbjct: 398 LYGVRLSPSSCANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVAPGCGPYH 457

Query: 421 PSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSI 480
           PSSVNFDSGVDDLHNPSQY V N+NMKSHIF EYVVSFKI SSP E G QDQLQ+NCSSI
Sbjct: 458 PSSVNFDSGVDDLHNPSQYLVSNVNMKSHIFPEYVVSFKIPSSPLELGRQDQLQINCSSI 517

Query: 481 ESVEDCVSSPVPEKRCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHY 540
           ES EDCVSSP P+KRC EK DHGSSSLKTPKSPWM F  L NAISK V LNDMKLV GHY
Sbjct: 518 ESEEDCVSSPAPKKRCLEKLDHGSSSLKTPKSPWMAFPRLLNAISKAVPLNDMKLVYGHY 577

Query: 541 CLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPPKSPCAFKVPKEEKEC 585
            LFREKK SRDEF+RRLRSIVGD LLRS LMSLQCK PPKSPC  KVPKEE EC
Sbjct: 578 GLFREKKTSRDEFVRRLRSIVGDQLLRSTLMSLQCKPPPKSPCTSKVPKEE-EC 620

BLAST of Carg25339 vs. ExPASy TrEMBL
Match: A0A6J1HJG7 (probable inactive poly [ADP-ribose] polymerase SRO1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111464106 PE=4 SV=1)

HSP 1 Score: 919.1 bits (2374), Expect = 9.4e-264
Identity = 455/473 (96.19%), Postives = 458/473 (96.83%), Query Frame = 0

Query: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTTHVGAGYEAFLSKLTSSRNRHAKCKRLDDC 60
           MNMETELTRVQHNGQKITVGLKRKRANQGT HVGAGY+AFLSKLTSSRNRHAKCKRLDDC
Sbjct: 1   MNMETELTRVQHNGQKITVGLKRKRANQGTAHVGAGYKAFLSKLTSSRNRHAKCKRLDDC 60

Query: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120
           EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV
Sbjct: 61  EVSRGPNSRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAAIEV 120

Query: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQHELEDAS 180
           ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQ+ELEDAS
Sbjct: 121 ELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHPFHQYELEDAS 180

Query: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKGKTFDIAEDWEDSE 240
           TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIK KTFDIAEDWEDSE
Sbjct: 181 TVALPDHTGMREINLHLEIGITEANSSQSEGYIEESNRDAKISNIKAKTFDIAEDWEDSE 240

Query: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKNIEILEVK 300
           TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIK+IEILEVK
Sbjct: 241 TNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGLDDVKNMFLMSMESIKDIEILEVK 300

Query: 301 RWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360
           RWSSHLMQDR DLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS
Sbjct: 301 RWSSHLMQDRPDLFKKQIEITKKSRGNANVQYGWLAGSMLHGVGHFEPKLTSTSGVHLSS 360

Query: 361 CANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420
           CANL           ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV
Sbjct: 361 CANLS----------ASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGV 420

Query: 421 DDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESV 474
           DDLHNPSQYFVWNMNMKSHIF EYVVSFKISSSPPECGLQDQLQLNCSSIESV
Sbjct: 421 DDLHNPSQYFVWNMNMKSHIFPEYVVSFKISSSPPECGLQDQLQLNCSSIESV 463

BLAST of Carg25339 vs. TAIR 10
Match: AT1G32230.2 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 287.7 bits (735), Expect = 2.1e-77
Identity = 206/602 (34.22%), Postives = 317/602 (52.66%), Query Frame = 0

Query: 3   METELTRVQHNGQ-KITVGLKRKRANQGTTHVGAGYEAFL-----SKLTSSRNRHAKCKR 62
           ME ++ +V  + + +   G KRKRA        A Y A++     +KL +    + +C+ 
Sbjct: 1   MEAKIVKVLDSSRCEDGFGKKRKRA--------ASYAAYVTGVSCAKLQNVPPPNGQCQI 60

Query: 63  LDDCEVSRGPN--------SRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIR 122
            D      G N        S ++ +R Y  F ++G+ KR + +E+G+W D P+  +  I+
Sbjct: 61  PDKRRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQ 120

Query: 123 EQFQIKNAAIEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVM 182
            + + K+AAIE +L G   +LD L+M +++++T  +  +AWID+ G+CFFPE Y  D   
Sbjct: 121 NELEEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERT 180

Query: 183 HPFHQHELEDASTVALPDHTGMREINLHLEIGIT--EANSSQSEGYIEESNRDAKISNIK 242
           +  H   +ED      P      +I L LEI +   E      E   +ES  D  + ++ 
Sbjct: 181 NYCHHKCVED------PKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESG-DNMMDDVP 240

Query: 243 GKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTV-GLDDVKNMFL 302
                  E ++++  +S     +++ AV    E D  V    ++  S V   D VK MF 
Sbjct: 241 LAQRSSNEHYDEATEDSCS--RKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFA 300

Query: 303 MSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW-------LAGS 362
           +   S+ ++ +L+V R+SS + + RL LF+KQ+EITKK RG+ANV+Y W       L+  
Sbjct: 301 VGTASLGHVPVLDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAV 360

Query: 363 MLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVI 422
           M+ G+   G F  K     G+HL++ A+  Y         A YCD DE GVRY+  C VI
Sbjct: 361 MMQGLGVGGAFIRKSIYGVGIHLTA-ADCPYF-------SARYCDVDENGVRYMVLCRVI 420

Query: 423 LGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSP- 482
           +GN+E++      +      +D+GVDD+ +P  Y VWN+NM +HIF E+VV FK+S+ P 
Sbjct: 421 MGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSNLPN 480

Query: 483 PECGLQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGS-----SSLKTPKSPWMPFSM 542
            E  L  +   +  ++E  +D      P+         GS     SS   PKSPWMPF  
Sbjct: 481 AEGNLIAKRDNSGVTLEGPKDL----PPQLESNGARGSGSANSVGSSTTRPKSPWMPFPT 540

Query: 543 LFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPP 572
           LF AIS +V+ NDM L+   Y   R+KK++R EF+R+LR IVGD LLRS + +LQ +  P
Sbjct: 541 LFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQ--P 571

BLAST of Carg25339 vs. TAIR 10
Match: AT1G32230.3 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 287.7 bits (735), Expect = 2.1e-77
Identity = 203/595 (34.12%), Postives = 313/595 (52.61%), Query Frame = 0

Query: 3   METELTRVQHNGQ-KITVGLKRKRANQGTTHVGAGYEAFL-----SKLTSSRNRHAKCKR 62
           ME ++ +V  + + +   G KRKRA        A Y A++     +KL +    + +C+ 
Sbjct: 1   MEAKIVKVLDSSRCEDGFGKKRKRA--------ASYAAYVTGVSCAKLQNVPPPNGQCQI 60

Query: 63  LDDCEVSRGPN--------SRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIR 122
            D      G N        S ++ +R Y  F ++G+ KR + +E+G+W D P+  +  I+
Sbjct: 61  PDKRRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQ 120

Query: 123 EQFQIKNAAIEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVM 182
            + + K+AAIE +L G   +LD L+M +++++T  +  +AWID+ G+CFFPE Y  D   
Sbjct: 121 NELEEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERT 180

Query: 183 HPFHQHELEDASTVALPDHTGMREINLHLEIGIT--EANSSQSEGYIEESNRDAKISNIK 242
           +  H   +ED      P      +I L LEI +   E      E   +ES  D  + ++ 
Sbjct: 181 NYCHHKCVED------PKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESG-DNMMDDVP 240

Query: 243 GKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTV-GLDDVKNMFL 302
                  E ++++  +S     +++ AV    E D  V    ++  S V   D VK MF 
Sbjct: 241 LAQRSSNEHYDEATEDSCS--RKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFA 300

Query: 303 MSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW-------LAGS 362
           +   S+ ++ +L+V R+SS + + RL LF+KQ+EITKK RG+ANV+Y W       L+  
Sbjct: 301 VGTASLGHVPVLDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAV 360

Query: 363 MLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVI 422
           M+ G+   G F  K     G+HL++ A+  Y         A YCD DE GVRY+  C VI
Sbjct: 361 MMQGLGVGGAFIRKSIYGVGIHLTA-ADCPYF-------SARYCDVDENGVRYMVLCRVI 420

Query: 423 LGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSP- 482
           +GN+E++      +      +D+GVDD+ +P  Y VWN+NM +HIF E+VV FK+S+ P 
Sbjct: 421 MGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSNLPN 480

Query: 483 PECGLQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGS-----SSLKTPKSPWMPFSM 542
            E  L  +   +  ++E  +D      P+         GS     SS   PKSPWMPF  
Sbjct: 481 AEGNLIAKRDNSGVTLEGPKDL----PPQLESNGARGSGSANSVGSSTTRPKSPWMPFPT 540

Query: 543 LFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQ 565
           LF AIS +V+ NDM L+   Y   R+KK++R EF+R+LR IVGD LLRS + +LQ
Sbjct: 541 LFAAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQ 566

BLAST of Carg25339 vs. TAIR 10
Match: AT1G32230.1 (WWE protein-protein interaction domain protein family )

HSP 1 Score: 287.3 bits (734), Expect = 2.7e-77
Identity = 203/600 (33.83%), Postives = 317/600 (52.83%), Query Frame = 0

Query: 3   METELTRVQHNGQ-KITVGLKRKRANQGTTHVGAGYEAFL-----SKLTSSRNRHAKCKR 62
           ME ++ +V  + + +   G KRKRA        A Y A++     +KL +    + +C+ 
Sbjct: 1   MEAKIVKVLDSSRCEDGFGKKRKRA--------ASYAAYVTGVSCAKLQNVPPPNGQCQI 60

Query: 63  LDDCEVSRGPN--------SRRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIR 122
            D      G N        S ++ +R Y  F ++G+ KR + +E+G+W D P+  +  I+
Sbjct: 61  PDKRRRLEGENKLSAYENRSGKALVRYYTYFKKTGIAKRVMMYENGEWNDLPEHVICAIQ 120

Query: 123 EQFQIKNAAIEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVM 182
            + + K+AAIE +L G   +LD L+M +++++T  +  +AWID+ G+CFFPE Y  D   
Sbjct: 121 NELEEKSAAIEFKLCGHSFILDFLHMQRLDMETGAKTPLAWIDNAGKCFFPEIYESDERT 180

Query: 183 HPFHQHELEDASTVALPDHTGMREINLHLEIGIT--EANSSQSEGYIEESNRDAKISNIK 242
           +  H   +ED      P      +I L LEI +   E      E   +ES  D  + ++ 
Sbjct: 181 NYCHHKCVED------PKQNAPHDIKLRLEIDVNGGETPRLNLEECSDESG-DNMMDDVP 240

Query: 243 GKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTV-GLDDVKNMFL 302
                  E ++++  +S     +++ AV    E D  V    ++  S V   D VK MF 
Sbjct: 241 LAQRSSNEHYDEATEDSCS--RKLEAAVSKWDETDAIVVSGAKLTGSEVLDKDAVKKMFA 300

Query: 303 MSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW-------LAGS 362
           +   S+ ++ +L+V R+SS + + RL LF+KQ+EITKK RG+ANV+Y W       L+  
Sbjct: 301 VGTASLGHVPVLDVGRFSSEIAEARLALFQKQVEITKKHRGDANVRYAWLPAKREVLSAV 360

Query: 363 MLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVRYIAFCHVI 422
           M+ G+   G F  K     G+HL++ A+  Y         A YCD DE GVRY+  C VI
Sbjct: 361 MMQGLGVGGAFIRKSIYGVGIHLTA-ADCPYF-------SARYCDVDENGVRYMVLCRVI 420

Query: 423 LGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVSFKISSSPP 482
           +GN+E++      +      +D+GVDD+ +P  Y VWN+NM +HIF E+VV FK+S+ P 
Sbjct: 421 MGNMELLRGDKAQFFSGGEEYDNGVDDIESPKNYIVWNINMNTHIFPEFVVRFKLSNLPN 480

Query: 483 ECG----LQDQLQLNCSSIESVEDCVSSPVPEKRCTEKHDHGSSSLKTPKSPWMPFSMLF 542
             G     +D   +     + +   + S    +     +  GSS+ + PKSPWMPF  LF
Sbjct: 481 AEGNLIAKRDNSGVTLEGPKDLPPQLESNQGARGSGSANSVGSSTTR-PKSPWMPFPTLF 540

Query: 543 NAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVGDPLLRSMLMSLQCKLPPKS 572
            AIS +V+ NDM L+   Y   R+KK++R EF+R+LR IVGD LLRS + +LQ +  PKS
Sbjct: 541 AAISHKVAENDMLLINADYQQLRDKKMTRAEFVRKLRVIVGDDLLRSTITTLQNQ--PKS 572

BLAST of Carg25339 vs. TAIR 10
Match: AT2G35510.1 (similar to RCD one 1 )

HSP 1 Score: 274.6 bits (701), Expect = 1.8e-73
Identity = 201/606 (33.17%), Postives = 304/606 (50.17%), Query Frame = 0

Query: 3   METELTRVQHNGQKITVGLKRKRANQGTTH-VGAGYEAFLSKLT--SSRNRHAKCKRLD- 62
           ME ++ +V  +  K  +G KRK     T +  G  Y      L+  SS  +  K + LD 
Sbjct: 1   MEAKIVKVSDSSYKDGLGKKRKHPGNYTPYDSGRSYAKLQWVLSPNSSTQKLEKRRNLDG 60

Query: 63  DCEVSRGPNS-RRSFLRNYKNFSRSGLPKRFLYHEDGDWVDYPQITVKLIREQFQIKNAA 122
           + +V    N   +S +R +  + ++G+PKR ++HE+G+W+D P   +  IR   + K A 
Sbjct: 61  ENKVIVSENHVEKSLVRYFSYYKKTGVPKRVMFHENGEWIDLPDHILCDIRNDLEAKRAT 120

Query: 123 IEVELNGRHLLLDALYMIQVELKTAIQQHIAWIDDNGQCFFPEFYSGDSVMHP-FHQHEL 182
           IE    GRH LLD L+M +++L+T ++  +AWID  G+CFFPE +  D++     H    
Sbjct: 121 IEFNWCGRHFLLDFLHMYRLDLETGVKTQLAWIDIAGKCFFPETF--DTLERDGCHHIRG 180

Query: 183 EDASTVALPDHTGMREINLHLEIG-------------ITEANSSQSEGY--IEES----N 242
           ED      P+    REI LH+EI              +T+ +    + +  ++ S    N
Sbjct: 181 ED------PEQHDQREIKLHIEIDVNSGELPRLNLNVVTDESGDNMDDFQAVQRSSNGPN 240

Query: 243 RDAKISNIKGKTFDIAEDWEDSETNSQKPVAQMQEAVGGIKEIDCYVTPQNEIIHSTVGL 302
            +A   +   +  D  E W+ +ET+    V   +E      E+D                
Sbjct: 241 DEASEDSCSRELDDAVEKWDKTETDRFSGVKPAEE------ELD---------------K 300

Query: 303 DDVKNMFLMSMESIKNIEILEVKRWSSHLMQDRLDLFKKQIEITKKSRGNANVQYGW--- 362
           D VK MF +   ++ ++E L+V ++SS + + RL LF+KQ +ITKK RG+AN++Y W   
Sbjct: 301 DAVKQMFALGAATLGHVESLDVYQFSSEIAKARLSLFQKQADITKKHRGDANIRYAWVPA 360

Query: 363 ----LAGSMLHGV---GHFEPKLTSTSGVHLSSCANLRYAFHLPSVKYASYCDDDEKGVR 422
               L+  M+HG+   G F  K     GVH ++C              A YCD D+ GVR
Sbjct: 361 KKEVLSAVMMHGLGVGGAFIKKSMYGVGVHAANCPYFS----------ARYCDIDDNGVR 420

Query: 423 YIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLHNPSQYFVWNMNMKSHIFQEYVVS 482
           ++  C VI+GN+E +      Y      +D+GVDD+ +P  Y +WNMNM +HI+ E+VVS
Sbjct: 421 HMVLCRVIMGNMEPLRGDNTQYFTGGEEYDNGVDDVESPKHYLIWNMNMNTHIYPEFVVS 480

Query: 483 FKISSSPPECGLQDQLQ-LNCSSIESVEDCVSSPVPEKRCTEKHDHG----SSSLKTPKS 542
           FK+S    E  +    Q  + SS  ++E    SP  E         G    SSS + P+S
Sbjct: 481 FKLSIPNAEGNILPTTQSRHESSGLTLEGPKGSPSNEPGRVSNGGSGSEKNSSSSRRPRS 540

Query: 543 PWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFIRRLRSIVG-DPLLRSMLM 568
           P MPF +LF AIS +++  DM L+   Y   REKK+SR EF + L  IVG D LL S + 
Sbjct: 541 PIMPFPLLFKAISSKIARKDMDLIIAGYQELREKKVSRKEFYKTLSMIVGDDDLLISTIT 567

BLAST of Carg25339 vs. TAIR 10
Match: AT1G23550.1 (similar to RCD one 2 )

HSP 1 Score: 130.2 bits (326), Expect = 5.5e-30
Identity = 87/256 (33.98%), Postives = 126/256 (49.22%), Query Frame = 0

Query: 320 ITKKSRGNANVQYGWLAGS-------MLHG-----VGHFEPKLTSTS-GVHL--SSCANL 379
           + +K  G+ANV+YGW AGS       + +G     VG FE    S   G+HL  S C   
Sbjct: 103 VARKHGGDANVKYGWYAGSRDEIQRIISYGFSNRDVGKFENDGGSHGIGIHLVPSKC--- 162

Query: 380 RYAFHLPSVKYASYCDDDEKGVRYIAFCHVILGNVEVVTPGCVPYHPSSVNFDSGVDDLH 439
                  S+  AS  + DE+G+RY+  C VILG  E++  G    +PSS  FDSGVDDLH
Sbjct: 163 -------SLLAASATEQDEEGLRYLLLCRVILGKPEIIISGSKQSYPSSAEFDSGVDDLH 222

Query: 440 NPSQYFVWNMNMKSHIFQEYVVSFKISSSPPECGLQDQLQLNCSSIESVEDCVSSPVPEK 499
           NP  Y +W+ NM S I   Y+VSF+   SP       +L+++     S            
Sbjct: 223 NPRNYVIWSCNMNSCILPSYIVSFR---SP-------RLRVSRGGFAS------------ 282

Query: 500 RCTEKHDHGSSSLKTPKSPWMPFSMLFNAISKEVSLNDMKLVKGHYCLFREKKISRDEFI 559
                          P SPW+ F+ L + +S  +  + M L+   Y  FR++KI RD+ +
Sbjct: 283 --------------RPSSPWVSFASLMSMLSTSMDPSRMNLIIRTYDDFRKRKIRRDQLV 312

Query: 560 RRLRSIVGDPLLRSML 561
           R++R + GD LL  ++
Sbjct: 343 RKMREVAGDNLLAEII 312

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7011369.10.0e+00100.00Inactive poly [ADP-ribose] polymerase RCD1, partial [Cucurbita argyrosperma subs... [more]
XP_023553913.10.0e+0097.09inactive poly [ADP-ribose] polymerase RCD1-like [Cucurbita pepo subsp. pepo] >XP... [more]
XP_022963963.10.0e+0096.58inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita moschata] ... [more]
KAG6571640.10.0e+0096.89Inactive poly [ADP-ribose] polymerase RCD1, partial [Cucurbita argyrosperma subs... [more]
XP_022967219.10.0e+0095.72inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 [Cucurbita maxima] >X... [more]
Match NameE-valueIdentityDescription
Q8RY593.8e-7633.83Inactive poly [ADP-ribose] polymerase RCD1 OS=Arabidopsis thaliana OX=3702 GN=RC... [more]
O822892.5e-7233.17Probable inactive poly [ADP-ribose] polymerase SRO1 OS=Arabidopsis thaliana OX=3... [more]
Q9ZUD97.7e-2933.98Probable inactive poly [ADP-ribose] polymerase SRO2 OS=Arabidopsis thaliana OX=3... [more]
O645922.6e-2429.93Probable inactive poly [ADP-ribose] polymerase SRO3 OS=Arabidopsis thaliana OX=3... [more]
Q9STU11.2e-2133.33Probable inactive poly [ADP-ribose] polymerase SRO4 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1HJF20.0e+0096.58inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Cucurbita moschata... [more]
A0A6J1HW580.0e+0095.72inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Cucurbita maxima O... [more]
A0A6J1E4V02.9e-26578.38inactive poly [ADP-ribose] polymerase RCD1-like isoform X1 OS=Momordica charanti... [more]
A0A0A0LE891.1e-26480.64Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G901000 PE=4 SV=1[more]
A0A6J1HJG79.4e-26496.19probable inactive poly [ADP-ribose] polymerase SRO1 isoform X2 OS=Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
AT1G32230.22.1e-7734.22WWE protein-protein interaction domain protein family [more]
AT1G32230.32.1e-7734.12WWE protein-protein interaction domain protein family [more]
AT1G32230.12.7e-7733.83WWE protein-protein interaction domain protein family [more]
AT2G35510.11.8e-7333.17similar to RCD one 1 [more]
AT1G23550.15.5e-3033.98similar to RCD one 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.228.10coord: 268..452
e-value: 4.9E-15
score: 57.9
NoneNo IPR availablePANTHERPTHR32263:SF24INACTIVE POLY [ADP-RIBOSE] POLYMERASE RCD1-LIKEcoord: 6..582
NoneNo IPR availableSUPERFAMILY56399ADP-ribosylationcoord: 278..449
IPR022003RST domainPFAMPF12174RSTcoord: 500..564
e-value: 5.2E-27
score: 93.5
IPR022003RST domainPROSITEPS51879RSTcoord: 498..569
score: 34.63171
IPR044964Inactive poly [ADP-ribose] polymerase RCD1/SRO1-5PANTHERPTHR32263INACTIVE POLY [ADP-RIBOSE] POLYMERASE SRO4-RELATEDcoord: 6..582
IPR004170WWE domainPROSITEPS50918WWEcoord: 74..149
score: 10.363427
IPR012317Poly(ADP-ribose) polymerase, catalytic domainPROSITEPS51059PARP_CATALYTICcoord: 248..469
score: 15.469231

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25339-RACarg25339-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003950 NAD+ ADP-ribosyltransferase activity