Carg25301 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25301
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DUF3754 domain-containing protein
Location	Carg_Chr11: 10462729 .. 10463465 (-)
RNA-Seq Expression	Carg25301
Synteny	Carg25301

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTTTTTTTCCCCTTGATGTTGTGAAGGTCAAAGAGGTGATCATTTCATTCTTTATACTGATGGAGCAAGGCAAAGCTACTTTAGCAGTATTGATCTTCTATCTCAACTTTATGTTCTTGCTCTTTTTTTGAATCGAAACCTCGATGTCGATTATCTGAAATTTTACGGTGAAATGTGATTAGGATCTTGATCTACGGTGTGAGGAGTTGATCAAAGAAGAGTTCGGTGAGCGTTGCAACTTCGAAGTGGACGATGCAGTTCAAAAGTTAGAGAAATTGGGAATCGTTTCACGGGTTAGTCTAAGCGTTTTTCTTATTGAAATCGAAGTATGACAACACGTGCATACACACACACACACACACACATACGTACGACTGCTGACTTTTTGGTTTATATAAGTTGATGGTGCACGGTATACTCGTGTGATTCGTTCTTCGTCGACTTTGATTTAGGATACCATTGGACGTTATTTCTGCGTTGGATTAAAACGTGCGAACGAGATCATTGGAGCGACGACAGAGGAGCTCGTCCTGAAAGCGAGGCAGGGTCTTAATCCTTGAAGTGAAAATGGTGCTTAGAAAATCATCCATTTCTGTAGCTTAGTGATGCTTGCTCTTGTAGTTTTGTTCTTGTATTTACCATAGAAATGCTACTCTTTGATGCGTTTCCAAGCTTGATTTGGGTATGATAAATGATACCAATAATATACACTTCAAAGGCTGCTTTTGTATTA

mRNA sequence

ATGCCTTTTTTTCCCCTTGATGTTGTGAAGGTCAAAGAGGTGATCATTTCATTCTTTATACTGATGGAGCAAGGCAAAGCTACTTTAGCAGATCTTGATCTACGGTGTGAGGAGTTGATCAAAGAAGAGTTCGGTGAGCGTTGCAACTTCGAAGTGGACGATGCAGTTCAAAAGTTAGAGAAATTGGGAATCGTTTCACGGGATACCATTGGACGTTATTTCTGCGTTGGATTAAAACGTGCGAACGAGATCATTGGAGCGACGACAGAGGAGCTCGTCCTGAAAGCGAGGCAGGGTCTTAATCCTTGAAGTGAAAATGGTGCTTAGAAAATCATCCATTTCTGTAGCTTAGTGATGCTTGCTCTTGTAGTTTTGTTCTTGTATTTACCATAGAAATGCTACTCTTTGATGCGTTTCCAAGCTTGATTTGGGTATGATAAATGATACCAATAATATACACTTCAAAGGCTGCTTTTGTATTA

Coding sequence (CDS)

ATGCCTTTTTTTCCCCTTGATGTTGTGAAGGTCAAAGAGGTGATCATTTCATTCTTTATACTGATGGAGCAAGGCAAAGCTACTTTAGCAGATCTTGATCTACGGTGTGAGGAGTTGATCAAAGAAGAGTTCGGTGAGCGTTGCAACTTCGAAGTGGACGATGCAGTTCAAAAGTTAGAGAAATTGGGAATCGTTTCACGGGATACCATTGGACGTTATTTCTGCGTTGGATTAAAACGTGCGAACGAGATCATTGGAGCGACGACAGAGGAGCTCGTCCTGAAAGCGAGGCAGGGTCTTAATCCTTGA

Protein sequence

MPFFPLDVVKVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDTIGRYFCVGLKRANEIIGATTEELVLKARQGLNP

Homology

[Close]

BLAST of Carg25301 vs. NCBI nr
Match: KAG7022617.1 (hypothetical protein SDJN02_16351, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 203.0 bits (515), Expect = 1.2e-48
Identity = 102/102 (100.00%), Postives = 102/102 (100.00%), Query Frame = 0


Query: 1   MPFFPLDVVKVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLE 60
           MPFFPLDVVKVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLE
Sbjct: 1   MPFFPLDVVKVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLE 60

Query: 61  KLGIVSRDTIGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           KLGIVSRDTIGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 61  KLGIVSRDTIGRYFCVGLKRANEIIGATTEELVLKARQGLNP 102

[Close]

BLAST of Carg25301 vs. NCBI nr
Match: XP_023531168.1 (uncharacterized protein LOC111793495 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 183.3 bits (464), Expect = 1.0e-42
Identity = 92/93 (98.92%), Postives = 93/93 (100.00%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT
Sbjct: 392 EVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 451

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 452 IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 484

[Close]

BLAST of Carg25301 vs. NCBI nr
Match: XP_022928407.1 (uncharacterized protein LOC111435231 [Cucurbita moschata] >XP_022928408.1 uncharacterized protein LOC111435231 [Cucurbita moschata])

HSP 1 Score: 182.2 bits (461), Expect = 2.3e-42
Identity = 91/93 (97.85%), Postives = 93/93 (100.00%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGK+TLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT
Sbjct: 392 EVKEVIISFFILMEQGKSTLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 451

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 452 IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 484

[Close]

BLAST of Carg25301 vs. NCBI nr
Match: XP_022989635.1 (uncharacterized protein LOC111486658 [Cucurbita maxima])

HSP 1 Score: 180.3 bits (456), Expect = 8.6e-42
Identity = 91/93 (97.85%), Postives = 92/93 (98.92%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATLADLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 392 EVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGECCNFEVDDAVQKLEKLGIVSRDT 451

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 452 IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 484

[Close]

BLAST of Carg25301 vs. NCBI nr
Match: XP_008449987.1 (PREDICTED: uncharacterized protein LOC103491708 isoform X2 [Cucumis melo])

HSP 1 Score: 176.4 bits (446), Expect = 1.2e-40
Identity = 88/93 (94.62%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATL DLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 391 EVKEVIISFFILMEQGKATLEDLDLRCEELIKEEFGEHCNFEVDDAVQKLEKLGIVSRDT 450

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRY+CVGLKRANEIIG TTEELVLKARQGLNP
Sbjct: 451 IGRYYCVGLKRANEIIGPTTEELVLKARQGLNP 483

[Close]

BLAST of Carg25301 vs. ExPASy TrEMBL
Match: A0A6J1ENX8 (uncharacterized protein LOC111435231 OS=Cucurbita moschata OX=3662 GN=LOC111435231 PE=4 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.1e-42
Identity = 91/93 (97.85%), Postives = 93/93 (100.00%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGK+TLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT
Sbjct: 392 EVKEVIISFFILMEQGKSTLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 451

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 452 IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 484

[Close]

BLAST of Carg25301 vs. ExPASy TrEMBL
Match: A0A6J1JMX5 (uncharacterized protein LOC111486658 OS=Cucurbita maxima OX=3661 GN=LOC111486658 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 4.2e-42
Identity = 91/93 (97.85%), Postives = 92/93 (98.92%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATLADLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 392 EVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGECCNFEVDDAVQKLEKLGIVSRDT 451

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRYFCVGLKRANEIIGATTEELVLKARQGLNP
Sbjct: 452 IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 484

[Close]

BLAST of Carg25301 vs. ExPASy TrEMBL
Match: A0A5A7U2V4 (DUF3754 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold61G002430 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.0e-41
Identity = 88/93 (94.62%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATL DLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 257 EVKEVIISFFILMEQGKATLEDLDLRCEELIKEEFGEHCNFEVDDAVQKLEKLGIVSRDT 316

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRY+CVGLKRANEIIG TTEELVLKARQGLNP
Sbjct: 317 IGRYYCVGLKRANEIIGPTTEELVLKARQGLNP 349

[Close]

BLAST of Carg25301 vs. ExPASy TrEMBL
Match: A0A5D3DUK4 (Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold236G00370 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.0e-41
Identity = 88/93 (94.62%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATL DLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 391 EVKEVIISFFILMEQGKATLEDLDLRCEELIKEEFGEHCNFEVDDAVQKLEKLGIVSRDT 450

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRY+CVGLKRANEIIG TTEELVLKARQGLNP
Sbjct: 451 IGRYYCVGLKRANEIIGPTTEELVLKARQGLNP 483

[Close]

BLAST of Carg25301 vs. ExPASy TrEMBL
Match: A0A1S3BNA9 (uncharacterized protein LOC103491708 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103491708 PE=4 SV=1)

HSP 1 Score: 176.4 bits (446), Expect = 6.0e-41
Identity = 88/93 (94.62%), Postives = 90/93 (96.77%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEVIISFFILMEQGKATL DLDLRCEELIKEEFGE CNFEVDDAVQKLEKLGIVSRDT
Sbjct: 391 EVKEVIISFFILMEQGKATLEDLDLRCEELIKEEFGEHCNFEVDDAVQKLEKLGIVSRDT 450

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRY+CVGLKRANEIIG TTEELVLKARQGLNP
Sbjct: 451 IGRYYCVGLKRANEIIGPTTEELVLKARQGLNP 483

[Close]

BLAST of Carg25301 vs. TAIR 10
Match: AT3G19340.1 (Protein of unknown function (DUF3754) )

HSP 1 Score: 162.5 bits (410), Expect = 1.7e-40
Identity = 78/93 (83.87%), Postives = 88/93 (94.62%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKATLADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRDT 69
           +VKEV+I F+ILMEQGKATL DLDLRCEELIKEEFG RCNF+V+DAVQKLEKLGIV+RDT
Sbjct: 394 EVKEVMICFYILMEQGKATLEDLDLRCEELIKEEFGARCNFDVEDAVQKLEKLGIVARDT 453

Query: 70  IGRYFCVGLKRANEIIGATTEELVLKARQGLNP 103
           IGRY+C+GLKRANEIIG TTEELVLKA+QG+ P
Sbjct: 454 IGRYYCMGLKRANEIIGTTTEELVLKAKQGVTP 486

[Close]

BLAST of Carg25301 vs. TAIR 10
Match: AT5G13940.1 (aminopeptidases )

HSP 1 Score: 115.5 bits (288), Expect = 2.4e-26
Identity = 58/91 (63.74%), Postives = 74/91 (81.32%), Query Frame = 0


Query: 10  KVKEVIISFFILMEQGKAT-LADLDLRCEELIKEEFGERCNFEVDDAVQKLEKLGIVSRD 69
           +VKEVIISFF+L+++G  T   +LD++ E  IKEEF E CNF+VDDA+ KLEKLG+VSRD
Sbjct: 679 EVKEVIISFFMLIKKGCPTSKEELDMKSEAFIKEEFNESCNFDVDDAITKLEKLGLVSRD 738

Query: 70  TIGRYFCVGLKRANEIIGATTEELVLKARQG 100
           +  +Y CV +K ANEI+G TTEE+VLKARQG
Sbjct: 739 SEDKYRCVEMKEANEIMGTTTEEMVLKARQG 769

The following BLAST results are available for this feature:

BLAST of Carg25301 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7022617.1	1.2e-48	100.00	hypothetical protein SDJN02_16351, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023531168.1	1.0e-42	98.92	uncharacterized protein LOC111793495 [Cucurbita pepo subsp. pepo]	[more]
XP_022928407.1	2.3e-42	97.85	uncharacterized protein LOC111435231 [Cucurbita moschata] >XP_022928408.1 unchar...	[more]
XP_022989635.1	8.6e-42	97.85	uncharacterized protein LOC111486658 [Cucurbita maxima]	[more]
XP_008449987.1	1.2e-40	94.62	PREDICTED: uncharacterized protein LOC103491708 isoform X2 [Cucumis melo]	[more]

BLAST of Carg25301 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg25301 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ENX8	1.1e-42	97.85	uncharacterized protein LOC111435231 OS=Cucurbita moschata OX=3662 GN=LOC1114352...	[more]
A0A6J1JMX5	4.2e-42	97.85	uncharacterized protein LOC111486658 OS=Cucurbita maxima OX=3661 GN=LOC111486658...	[more]
A0A5A7U2V4	6.0e-41	94.62	DUF3754 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A5D3DUK4	6.0e-41	94.62	Uncharacterized protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BNA9	6.0e-41	94.62	uncharacterized protein LOC103491708 isoform X2 OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg25301 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19340.1	1.7e-40	83.87	Protein of unknown function (DUF3754)	[more]
AT5G13940.1	2.4e-26	63.74	aminopeptidases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 22..42
None	No IPR available	PANTHER	PTHR33645:SF8	SUBFAMILY NOT NAMED	coord: 10..99
None	No IPR available	PANTHER	PTHR33645	AMINOPEPTIDASE (DUF3754)	coord: 10..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25301-RA	Carg25301-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane