Carg25274 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25274
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF-hand family protein
Location	Carg_Chr16: 8438258 .. 8438701 (-)
RNA-Seq Expression	Carg25274
Synteny	Carg25274

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGATCTGAGCGATGAACAGAAATCCTCCATGAAGGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCTGAGGAGAGTCTTACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCCTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTCGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

mRNA sequence

ATGGGCAAAGATCTGAGCGATGAACAGAAATCCTCCATGAAGGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCTGAGGAGAGTCTTACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCCTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTCGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCGATGAACAGAAATCCTCCATGAAGGAGGCCTTTACCCTCTTCGATTCCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATTCTAATGCGTTCCCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCTGAGGAGAGTCTTACTGCTCCTTTTGATTTCAATCGCTTTCTTGAGATCATGTCCAAGCACATGAAGCATGAGCCCTTCGATCGCCAGCTTCGAGACGCCTTTAAGGTTCTCGATAAAGAGAACACCGGTTACGTCCTGGTCTCGGAGCTCCGACACATTTTGACGAGTATTGGTGAGAAGCTTGAGCCGTCGGAGTTTGATGAGTGGATTCGAGAAGTTGAGGTCGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Protein sequence

MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGPDGKIRYEDFIARMVAK

Homology

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BLAST of Carg25274 vs. NCBI nr
Match: KAG6577598.1 (putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015647.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 293.5 bits (750), Expect = 1.0e-75
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. NCBI nr
Match: XP_022923443.1 (probable calcium-binding protein CML13 [Cucurbita moschata] >XP_022923444.1 probable calcium-binding protein CML13 [Cucurbita moschata] >XP_023553502.1 probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 292.4 bits (747), Expect = 2.2e-75
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. NCBI nr
Match: XP_022965210.1 (probable calcium-binding protein CML13 [Cucurbita maxima])

HSP 1 Score: 289.3 bits (739), Expect = 1.9e-74
Identity = 145/147 (98.64%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
            FDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  SFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 279.6 bits (714), Expect = 1.5e-71
Identity = 139/147 (94.56%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSD+QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMK EPFDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. NCBI nr
Match: XP_023540891.1 (probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028366.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 278.9 bits (712), Expect = 2.6e-71
Identity = 139/147 (94.56%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLS++QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMK E FDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 9.8e-66
Identity = 123/148 (83.11%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           MGKD LSD+Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg25274 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 242.3 bits (617), Expect = 3.5e-63
Identity = 118/148 (79.73%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           M KD LS++Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg25274 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 1.5e-58
Identity = 108/146 (73.97%), Postives = 130/146 (89.04%), Query Frame = 0


Query: 2   GKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTAP 61
           GK+LS+EQ +SM+EAF+LFD+DGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LTAP
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++ EPFDR LRDAF+VLDK+ +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGPDGKIRYEDFIARMVAK 148
           EWIREV+V PDG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

[Close]

BLAST of Carg25274 vs. ExPASy Swiss-Prot
Match: P84339 (Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-34
Identity = 72/149 (48.32%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   LS+EQ S  KEAF+LFD DGDG I   ELG +MRSLG NP+QA+L+ +I E     
Sbjct: 1   MADQLSEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPSQAELEDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ M+    + ++++AFKV DK+  GY+  +ELRH++T++GEKL  S
Sbjct: 61  NGTIDFPEFLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDS 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M++K
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKMMLSK 149

[Close]

BLAST of Carg25274 vs. ExPASy Swiss-Prot
Match: P13868 (Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2)

HSP 1 Score: 146.0 bits (367), Expect = 3.4e-34
Identity = 69/149 (46.31%), Postives = 102/149 (68.46%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M + L++EQ +  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I+E     
Sbjct: 1   MAEQLTEEQIAEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMISEADADQ 60

Query: 61  TAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AFKV DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFKVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE ++  DG++ YE+F+  M+AK
Sbjct: 121 EVDEMIREADIDGDGQVNYEEFVRMMLAK 149

[Close]

BLAST of Carg25274 vs. ExPASy TrEMBL
Match: A0A6J1E9N4 (probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC111431138 PE=4 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 1.1e-75
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. ExPASy TrEMBL
Match: A0A6J1HN38 (probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC111465135 PE=4 SV=1)

HSP 1 Score: 289.3 bits (739), Expect = 9.2e-75
Identity = 145/147 (98.64%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
            FDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  SFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. ExPASy TrEMBL
Match: A0A6J1L2L8 (probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC111498532 PE=4 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 2.8e-71
Identity = 138/147 (93.88%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLS++QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LT+
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMK E FDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. ExPASy TrEMBL
Match: A0A6J1FT67 (probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC111448243 PE=4 SV=1)

HSP 1 Score: 276.6 bits (706), Expect = 6.2e-71
Identity = 138/147 (93.88%), Postives = 144/147 (97.96%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLS++QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEE+LTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFLEIMSKHMK E FDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFTRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. ExPASy TrEMBL
Match: A0A6J1DIL4 (probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC111020377 PE=4 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 8.1e-71
Identity = 136/147 (92.52%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTA 60
           MGKDLSD+QKSSMKEAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT+
Sbjct: 1   MGKDLSDDQKSSMKEAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLTS 60

Query: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL++MSKHMK EPFDRQLRDAFKVLDK+NTG+VLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDLMSKHMKPEPFDRQLRDAFKVLDKDNTGFVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREV+VG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVDVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Carg25274 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 250.8 bits (639), Expect = 7.0e-67
Identity = 123/148 (83.11%), Postives = 140/148 (94.59%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           MGKD LSD+Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPTQAQLK+IIA E+L+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg25274 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 242.3 bits (617), Expect = 2.5e-64
Identity = 118/148 (79.73%), Postives = 138/148 (93.24%), Query Frame = 0


Query: 1   MGKD-LSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESLT 60
           M KD LS++Q SSMKEAF LFD+DGDG+IAPSELGILMRSLGGNPT++QLK+II  E+L+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K EPFDRQLRDAFKVLDKE TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg25274 vs. TAIR 10
Match: AT1G66410.1 (calmodulin 4 )

HSP 1 Score: 144.8 bits (364), Expect = 5.4e-35
Identity = 72/149 (48.32%), Postives = 100/149 (67.11%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DEQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M+K MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E +E IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVEEMIREADVDGDGQINYEEFVKIMMAK 149

[Close]

BLAST of Carg25274 vs. TAIR 10
Match: AT5G37780.1 (calmodulin 1 )

HSP 1 Score: 144.8 bits (364), Expect = 5.4e-35
Identity = 72/149 (48.32%), Postives = 100/149 (67.11%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+DEQ S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDEQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M+K MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMAKKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E +E IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVEEMIREADVDGDGQINYEEFVKIMMAK 149

[Close]

BLAST of Carg25274 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 144.4 bits (363), Expect = 7.1e-35
Identity = 71/149 (47.65%), Postives = 100/149 (67.11%), Query Frame = 0


Query: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEESL-- 60
           M   L+D+Q S  KEAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E     
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DK+  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of Carg25274 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6577598.1	1.0e-75	100.00	putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_022923443.1	2.2e-75	99.32	probable calcium-binding protein CML13 [Cucurbita moschata] >XP_022923444.1 prob...	[more]
XP_022965210.1	1.9e-74	98.64	probable calcium-binding protein CML13 [Cucurbita maxima]	[more]
XP_038876882.1	1.5e-71	94.56	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_023540891.1	2.6e-71	94.56	probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839....	[more]

BLAST of Carg25274 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	9.8e-66	83.11	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	3.5e-63	79.73	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	1.5e-58	73.97	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P84339	2.6e-34	48.32	Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2	[more]
P13868	3.4e-34	46.31	Calmodulin-1 OS=Solanum tuberosum OX=4113 GN=PCM1 PE=1 SV=2	[more]

BLAST of Carg25274 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E9N4	1.1e-75	99.32	probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HN38	9.2e-75	98.64	probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC1114651...	[more]
A0A6J1L2L8	2.8e-71	93.88	probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC1114985...	[more]
A0A6J1FT67	6.2e-71	93.88	probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1DIL4	8.1e-71	92.52	probable calcium-binding protein CML13 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Carg25274 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	7.0e-67	83.11	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	2.5e-64	79.73	Calcium-binding EF-hand family protein	[more]
AT1G66410.1	5.4e-35	48.32	calmodulin 4	[more]
AT5G37780.1	5.4e-35	48.32	calmodulin 1	[more]
AT5G21274.1	7.1e-35	47.65	calmodulin 6	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 119..147 e-value: 6.9 score: 11.0 coord: 12..40 e-value: 3.0E-4 score: 30.1 coord: 83..111 e-value: 0.013 score: 24.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 3.7E-12 score: 46.5
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 9.3E-6 score: 25.1
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 11.082272
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.811786
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..72 e-value: 1.53551E-8 score: 46.3869
None	No IPR available	GENE3D	1.10.238.10		coord: 81..145 e-value: 5.4E-18 score: 66.9
None	No IPR available	GENE3D	1.10.238.10		coord: 3..80 e-value: 1.7E-20 score: 75.0
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25274-RA	Carg25274-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding