Carg25239 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25239 vs. NCBI nr
Match: KAG7032577.1 (hypothetical protein SDJN02_06626, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 376.3 bits (965), Expect = 1.5e-100
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0

Query: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLLYKQKKNQNVK 60
           MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLLYKQKKNQNVK
Sbjct: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLLYKQKKNQNVK 60

Query: 61  GNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS 120
           GNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS
Sbjct: 61  GNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS 120

Query: 121 AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN 180
           AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN
Sbjct: 121 AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN 180

Query: 181 MKIYSH 187
           MKIYSH
Sbjct: 181 MKIYSH 186

BLAST of Carg25239 vs. NCBI nr
Match: KAG6579252.1 (hypothetical protein SDJN03_23700, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 299.7 bits (766), Expect = 1.8e-77
Identity = 156/197 (79.19%), Postives = 163/197 (82.74%), Query Frame = 0

Query: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLL-------YKQ 60
           MVEENS SSNYMRSLLDSRASARLPLSA SSFAFGSCKEGW       L        YK+
Sbjct: 1   MVEENSCSSNYMRSLLDSRASARLPLSAASSFAFGSCKEGWLSSVADSLFLGYNLKKYKK 60

Query: 61  ----KKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGS 120
               KKNQNVKGNRLLIS+NVLGSAGPIRFIV+EEQLVA VIDTALKSYAREGRLPILGS
Sbjct: 61  GFGLKKNQNVKGNRLLISVNVLGSAGPIRFIVNEEQLVAAVIDTALKSYAREGRLPILGS 120

Query: 121 DLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASG 180
           DL DFQLYCP AGPDALSPWDTIGSHG+RNF LCKKPQ EK GE G +   P+I RK +G
Sbjct: 121 DLRDFQLYCPIAGPDALSPWDTIGSHGSRNFMLCKKPQPEKVGEHGNSPPEPSIPRKGTG 180

Query: 181 SWKSWINKSLNMKIYSH 187
           SWKSWINKSLNMKIYSH
Sbjct: 181 SWKSWINKSLNMKIYSH 197

BLAST of Carg25239 vs. NCBI nr
Match: KAG6601876.1 (hypothetical protein SDJN03_07109, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 292.4 bits (747), Expect = 2.8e-75
Identity = 153/186 (82.26%), Postives = 153/186 (82.26%), Query Frame = 0

Query: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLLYKQKKNQNVK 60
           MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKE                     
Sbjct: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKE--------------------- 60

Query: 61  GNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS 120
                      G AGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS
Sbjct: 61  -----------GCAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS 120

Query: 121 AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN 180
           AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN
Sbjct: 121 AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN 154

Query: 181 MKIYSH 187
           MKIYSH
Sbjct: 181 MKIYSH 154

BLAST of Carg25239 vs. NCBI nr
Match: XP_022959923.1 (uncharacterized protein At4g22758-like [Cucurbita moschata])

HSP 1 Score: 284.6 bits (727), Expect = 5.9e-73
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL
Sbjct: 3   MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 62

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 167
           GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA
Sbjct: 63  GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 122

Query: 168 SGSWKSWINKSLNMKIYSH 187
           SGSWKSWINKSLNMKIYSH
Sbjct: 123 SGSWKSWINKSLNMKIYSH 141

BLAST of Carg25239 vs. NCBI nr
Match: XP_023521148.1 (uncharacterized protein At4g22758-like [Cucurbita pepo subsp. pepo] >XP_023521149.1 uncharacterized protein At4g22758-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 283.1 bits (723), Expect = 1.7e-72
Identity = 138/139 (99.28%), Postives = 138/139 (99.28%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL
Sbjct: 3   MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 62

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 167
           GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEK GEDGKAASRPAIHRKA
Sbjct: 63  GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKVGEDGKAASRPAIHRKA 122

Query: 168 SGSWKSWINKSLNMKIYSH 187
           SGSWKSWINKSLNMKIYSH
Sbjct: 123 SGSWKSWINKSLNMKIYSH 141

BLAST of Carg25239 vs. ExPASy Swiss-Prot
Match: Q56XJ7 (Uncharacterized protein At4g22758 OS=Arabidopsis thaliana OX=3702 GN=At4g22758 PE=2 SV=1)

HSP 1 Score: 48.1 bits (113), Expect = 1.2e-04
Identity = 31/93 (33.33%), Postives = 47/93 (50.54%), Query Frame = 0

Query: 58  NVKGN-----RLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLH 117
           N +GN     +++IS+ V GS GP+R +V     V + I   +  Y +EGR P L  D  
Sbjct: 113 NQEGNTKEAPKVIISVAVEGSPGPVRAMVKLSCNVEETIKIVVDKYCKEGRTPKLDRD-S 172

Query: 118 DFQLYCPSAGPDALSPWDTIGSHGARNFTLCKK 146
            F+L+        L   + IG  G+R+F + KK
Sbjct: 173 AFELHQSHFSIQCLEKREIIGELGSRSFYMRKK 204

BLAST of Carg25239 vs. ExPASy TrEMBL
Match: A0A6J1H7B9 (uncharacterized protein At4g22758-like OS=Cucurbita moschata OX=3662 GN=LOC111460829 PE=4 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.9e-73
Identity = 139/139 (100.00%), Postives = 139/139 (100.00%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL
Sbjct: 3   MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 62

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 167
           GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA
Sbjct: 63  GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 122

Query: 168 SGSWKSWINKSLNMKIYSH 187
           SGSWKSWINKSLNMKIYSH
Sbjct: 123 SGSWKSWINKSLNMKIYSH 141

BLAST of Carg25239 vs. ExPASy TrEMBL
Match: A0A6J1JP66 (uncharacterized protein At4g22758 OS=Cucurbita maxima OX=3661 GN=LOC111487636 PE=4 SV=1)

HSP 1 Score: 280.4 bits (716), Expect = 5.4e-72
Identity = 137/139 (98.56%), Postives = 137/139 (98.56%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL
Sbjct: 3   MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 62

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 167
           GSDLH FQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEK GEDGKAASRPAIHRKA
Sbjct: 63  GSDLHHFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKVGEDGKAASRPAIHRKA 122

Query: 168 SGSWKSWINKSLNMKIYSH 187
           SGSWKSWINKSLNMKIYSH
Sbjct: 123 SGSWKSWINKSLNMKIYSH 141

BLAST of Carg25239 vs. ExPASy TrEMBL
Match: A0A2N9ETJ3 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS5995 PE=4 SV=1)

HSP 1 Score: 258.8 bits (660), Expect = 1.7e-65
Identity = 135/189 (71.43%), Postives = 151/189 (79.89%), Query Frame = 0

Query: 4   ENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLG------MLLYKQKKNQ 63
           E SSSSN M SL  +RASA +  +   + AFGSCKEGWYYRCLG      MLLYK KKNQ
Sbjct: 3   EISSSSNLMLSLFQARASAVIAFA--FAVAFGSCKEGWYYRCLGYLFFATMLLYKPKKNQ 62

Query: 64  NVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLY 123
           N K NRLLISI VLGSAGPIRF+V+E++LVA VIDTALKSYAREGRLPILGS+L++F LY
Sbjct: 63  NAKANRLLISITVLGSAGPIRFVVNEDELVAAVIDTALKSYAREGRLPILGSNLNEFILY 122

Query: 124 CPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINK 183
           CP+AGPDALSPWDTIGS G RNF LCKKPQ  K   D + + RP I RK SGSWK+WINK
Sbjct: 123 CPNAGPDALSPWDTIGSQGVRNFMLCKKPQPVKMAGDDETSIRP-ISRKGSGSWKAWINK 182

Query: 184 SLNMKIYSH 187
           SLN+KIYSH
Sbjct: 183 SLNLKIYSH 188

BLAST of Carg25239 vs. ExPASy TrEMBL
Match: A0A5D3E3V5 (Senescence-associated protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold426G00320 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 9.3e-64
Identity = 133/186 (71.51%), Postives = 140/186 (75.27%), Query Frame = 0

Query: 1   MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGMLLYKQKKNQNVK 60
           MVEENSSSSNYMRSLLDSRASARLPLSA SSFAFGSCKE                     
Sbjct: 1   MVEENSSSSNYMRSLLDSRASARLPLSAASSFAFGSCKE--------------------- 60

Query: 61  GNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILGSDLHDFQLYCPS 120
                      G AGPIRFIV+EEQLVA VIDTALKSYAREGRLPILGSDL DFQLYCP+
Sbjct: 61  -----------GCAGPIRFIVNEEQLVAAVIDTALKSYAREGRLPILGSDLRDFQLYCPN 120

Query: 121 AGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKASGSWKSWINKSLN 180
            GPDALSPWDTIGSHGARNF LCKKPQ EK  E+GK+   P I RK++GSWKSWINKSLN
Sbjct: 121 TGPDALSPWDTIGSHGARNFMLCKKPQPEKVSEEGKSPPGPTIPRKSAGSWKSWINKSLN 154

Query: 181 MKIYSH 187
           +KIYSH
Sbjct: 181 LKIYSH 154

BLAST of Carg25239 vs. ExPASy TrEMBL
Match: A0A0A0KUW8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G569870 PE=4 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 4.6e-63
Identity = 122/139 (87.77%), Postives = 128/139 (92.09%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIV+EEQLVA VIDTALKSYAREGRLPIL
Sbjct: 1   MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVNEEQLVAAVIDTALKSYAREGRLPIL 60

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRKA 167
           GSDL DFQLYCP+ GPDALSPWDTIGSHGARNF LCKKPQ EK  E+GK  S PAI RK+
Sbjct: 61  GSDLRDFQLYCPNTGPDALSPWDTIGSHGARNFMLCKKPQPEKVPEEGKTPSGPAIPRKS 120

Query: 168 SGSWKSWINKSLNMKIYSH 187
           +GSWKSWINKSLN+KIYSH
Sbjct: 121 AGSWKSWINKSLNLKIYSH 139

BLAST of Carg25239 vs. TAIR 10
Match: AT1G70780.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: sperm cell, male gametophyte, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23150.1); Has 143 Blast hits to 143 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 143; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 183.7 bits (465), Expect = 1.3e-46
Identity = 90/140 (64.29%), Postives = 105/140 (75.00%), Query Frame = 0

Query: 48  MLLY-KQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPI 107
           MLLY KQKKNQN KGNR+LIS+ VLGSAGPIRF+  E+ LVA VIDTALK YAREGRLP+
Sbjct: 1   MLLYNKQKKNQNAKGNRILISVTVLGSAGPIRFVAYEDDLVASVIDTALKGYAREGRLPL 60

Query: 108 LGSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIHRK 167
           LGSD +DF LYCP  GP+ALS WD IGS GARNF LC+KP+ +K  E    +       +
Sbjct: 61  LGSDFNDFLLYCPMVGPEALSTWDAIGSLGARNFMLCRKPEEKKVEESNGRSDSTINGAR 120

Query: 168 ASGSWKSWINKSLNMKIYSH 187
             GS K+WINKS N+K+ SH
Sbjct: 121 KGGSLKAWINKSFNLKVSSH 140

BLAST of Carg25239 vs. TAIR 10
Match: AT1G23150.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G70780.1); Has 124 Blast hits to 124 proteins in 17 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 124; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 175.6 bits (444), Expect = 3.6e-44
Identity = 91/142 (64.08%), Postives = 105/142 (73.94%), Query Frame = 0

Query: 48  MLLYKQKKNQNVKGNRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPIL 107
           MLLYK KKNQ VKGNR+LIS+  LGSAGPIRF+ +E  LVA VIDTALK YAREGRLPIL
Sbjct: 1   MLLYK-KKNQIVKGNRILISVTFLGSAGPIRFVANEGDLVASVIDTALKCYAREGRLPIL 60

Query: 108 GSDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAIH--- 167
           GSD +DF  YCP  GP ALSPW+ IGS G RNF LCKK   EK  E+ K  S   I+   
Sbjct: 61  GSDFNDFVFYCPMVGPGALSPWEAIGSVGVRNFMLCKKKPEEKKVEEDKGRSNFPINGAR 120

Query: 168 RKASGSWKSWINKSLNMKIYSH 187
           + A GS+K+WINKSL +K+ +H
Sbjct: 121 KGAGGSFKAWINKSLRLKVTTH 141

BLAST of Carg25239 vs. TAIR 10
Match: AT5G37730.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G23150.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 99.4 bits (246), Expect = 3.3e-21
Identity = 51/115 (44.35%), Postives = 72/115 (62.61%), Query Frame = 0

Query: 55  KNQNVKG------NRLLISINVLGSAGPIRFIVSEEQLVADVIDTALKSYAREGRLPILG 114
           +N+NVKG       +LL+S+NVLGS GPIRF+ +E+  V+  I+T LK+YAR+GR+P+LG
Sbjct: 3   RNENVKGVMKRKNKKLLVSVNVLGSVGPIRFLANEDDEVSSAINTTLKAYARQGRIPVLG 62

Query: 115 SDLHDFQLYCPSAGPDALSPWDTIGSHGARNFTLCKKPQAEKAGEDGKAASRPAI 164
            D+ +F  Y  +AG + L P + IGS    NF +CKK        +G   SR  I
Sbjct: 63  FDVDNFIFYSINAGFNTLHPQEKIGSMDVTNFLMCKKEPRPLEKVEGIRESRARI 117

BLAST of Carg25239 vs. TAIR 10
Match: AT1G70782.1 (conserved peptide upstream open reading frame 28 )

HSP 1 Score: 57.8 bits (138), Expect = 1.1e-08
Identity = 29/48 (60.42%), Postives = 32/48 (66.67%), Query Frame = 0

Query: 1  MVEENSSSSNYMRSLLDSRASARLPLSAFSSFAFGSCKEGWYYRCLGM 49
          MVE N     +  SL   RAS  LPLS  S FAFGSCKEGWY+RCLG+
Sbjct: 1  MVETNPIGGKFTLSLSIKRASFSLPLSTSSVFAFGSCKEGWYFRCLGL 48

BLAST of Carg25239 vs. TAIR 10
Match: AT1G23149.1 (conserved peptide upstream open reading frame 29 )

HSP 1 Score: 50.4 bits (119), Expect = 1.8e-06
Identity = 26/49 (53.06%), Postives = 33/49 (67.35%), Query Frame = 0

Query: 1  MVEENSSSSNYMRSLLDSRAS-ARLPLSAFSSFAFGSCKEGWYYRCLGM 49
          MVE N    N+  +L   RAS   +PLS  S F+FGSCKEGWY+RC+G+
Sbjct: 1  MVETNLIGDNHRLTLSTKRASFLIIPLSQDSVFSFGSCKEGWYFRCIGL 49