Carg25162 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25162 vs. NCBI nr
Match: KAG7028378.1 (Oxysterol-binding protein-related protein 2A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1541.9 bits (3991), Expect = 0.0e+00
Identity = 756/756 (100.00%), Postives = 756/756 (100.00%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI
Sbjct: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 756

BLAST of Carg25162 vs. NCBI nr
Match: XP_022942973.1 (oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita moschata] >XP_022942982.1 oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1534.6 bits (3972), Expect = 0.0e+00
Identity = 751/756 (99.34%), Postives = 754/756 (99.74%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVS QKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI
Sbjct: 181 DNLPFVPNDLSVSAQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIE+EANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIELEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDT ESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTNESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 756

BLAST of Carg25162 vs. NCBI nr
Match: XP_023539848.1 (oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023539849.1 oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1515.0 bits (3921), Expect = 0.0e+00
Identity = 744/756 (98.41%), Postives = 750/756 (99.21%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRS LI
Sbjct: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSSLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIEIEANGIHNGEYQLMKH+FSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIEIEANGIHNGEYQLMKHEFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDT ESFTEVTLG GSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTNESFTEVTLGRGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHL+DQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLVDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYV+GESNGNPNRSSSSD SMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVEGESNGNPNRSSSSDVSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGED DEP
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDTDEP 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGEL+ENLV ESGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELSENLVGESGS 756

BLAST of Carg25162 vs. NCBI nr
Match: XP_023005305.1 (oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita maxima] >XP_023005306.1 oxysterol-binding protein-related protein 2A-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 740/756 (97.88%), Postives = 747/756 (98.81%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSN +TQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNTSTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI
Sbjct: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDT ESFTE TLG GSVKGA DSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTNESFTEFTLGYGSVKGAFDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKV++FAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVASFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGF+EDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYV+GESNGNPNRSSSSD SMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVEGESNGNPNRSSSSDVSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGED DE 
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDTDEA 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGELNENLV +SGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVGDSGS 756

BLAST of Carg25162 vs. NCBI nr
Match: KAG6596849.1 (Oxysterol-binding protein-related protein 2A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1442.2 bits (3732), Expect = 0.0e+00
Identity = 713/751 (94.94%), Postives = 716/751 (95.34%), Query Frame = 0

Query: 6   MHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRVAGVLY 65
           MHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRVAGVLY
Sbjct: 1   MHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRVAGVLY 60

Query: 66  KWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKSGGIVH 125
           KWTNYGKGWRSRWF+LRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKSGGIVH
Sbjct: 61  KWTNYGKGWRSRWFLLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKSGGIVH 120

Query: 126 LKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILNDNLPF 185
           LKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDR                         
Sbjct: 121 LKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDR------------------------- 180

Query: 186 VPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLIDTLRE 245
                     KLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLIDTLRE
Sbjct: 181 ----------KLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLIDTLRE 240

Query: 246 FEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSDVDESF 305
           FEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSDVDESF
Sbjct: 241 FEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSDVDESF 300

Query: 306 FYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQRRKKL 365
           FYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQRRKKL
Sbjct: 301 FYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQRRKKL 360

Query: 366 PDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHG 425
           PDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHG
Sbjct: 361 PDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHG 420

Query: 426 IQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPT 485
           IQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPT
Sbjct: 421 IQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPT 480

Query: 486 VIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLIL 545
           VIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLIL
Sbjct: 481 VIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLIL 540

Query: 546 GKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEG 605
           GKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEG
Sbjct: 541 GKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEG 600

Query: 606 MYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTD 665
           MYYV+GESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTD
Sbjct: 601 MYYVEGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTD 660

Query: 666 SRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMG 725
           SRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMG
Sbjct: 661 SRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMG 716

Query: 726 GYWEARKERKWDGCLDVFGELNENLVDESGS 757
           GYWEARKERKWDGCLDVFGELNENLVDESGS
Sbjct: 721 GYWEARKERKWDGCLDVFGELNENLVDESGS 716

BLAST of Carg25162 vs. ExPASy Swiss-Prot
Match: Q940Y1 (Oxysterol-binding protein-related protein 2A OS=Arabidopsis thaliana OX=3702 GN=ORP2A PE=2 SV=1)

HSP 1 Score: 985.7 bits (2547), Expect = 2.9e-286
Identity = 506/770 (65.71%), Postives = 597/770 (77.53%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEE--ASSLSRSRSLPV-SFPAFSNANTQQISRSV 60
           MRVKE+HPLCCI+LE+   GI  QSPEE   ++L+RSRSLP  S    S + T       
Sbjct: 1   MRVKELHPLCCITLES-PHGINSQSPEEPVTTALTRSRSLPAKSLTGGSESET------- 60

Query: 61  DRVAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI------SLLSQPNDDVQLIGKISTNR 120
             VAG+LYKWTN+GKGWRSRWF+LR+G+LSYSKI      +LLS P+DDV+LIG IS  R
Sbjct: 61  --VAGILYKWTNFGKGWRSRWFLLRNGILSYSKIRRPENLNLLS-PDDDVRLIGDISGER 120

Query: 121 LS-------RRKQQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWI 180
           LS       RRKQQK+ GIVHLKVSSFRESKSDDR+FYIFTATKTLHLRT+S  DR  W+
Sbjct: 121 LSRMDSCSGRRKQQKTVGIVHLKVSSFRESKSDDRKFYIFTATKTLHLRTDSITDRAAWL 180

Query: 181 QALASSRSLFPLRILNDNLPFV-PNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFS 240
           QALAS++ +FPLR LN +  F+ P DLS+ST++L+KRL EEG+++ +VK+CEQI+LSEFS
Sbjct: 181 QALASTKCIFPLRSLNGDFSFIPPKDLSISTERLKKRLHEEGMNENLVKECEQIMLSEFS 240

Query: 241 EMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECAT 300
           EM GQ+K+L E+R+ L+D LR+ EAAN+E+ A+G         KH+FS+   GKYSEC+T
Sbjct: 241 EMHGQVKLLHEERTNLLDALRQLEAANLEVGASG---------KHEFSSLGRGKYSECST 300

Query: 301 TESSDDVEKQDLDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNA 360
           T SSD   KQ+ +D S+ DE  F+DTKE F E  +G  S    S                
Sbjct: 301 TASSDG--KQEFEDVSEEDEPSFHDTKEFFNEPNIGSESNLAISG--------------- 360

Query: 361 EKMHDEQIENFVHSNVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPIS 420
                       H++++RR KLPDP EKEK VSLWSMIKDNVGKDL RVCLPVYFNEPIS
Sbjct: 361 ------------HTDIKRRTKLPDPAEKEKGVSLWSMIKDNVGKDLTRVCLPVYFNEPIS 420

Query: 421 SLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGET 480
           SLQKC ED+EYS+LLD+AYEHG  GN LLR L V+AFAVSGYAS+EGRHCKPFNPLLGET
Sbjct: 421 SLQKCFEDLEYSYLLDRAYEHGKSGNGLLRALNVAAFAVSGYASTEGRHCKPFNPLLGET 480

Query: 481 YEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNL 540
           YEADFPEKGIRFFSEKVSHHPTVIACHC G+GWKFWG++NLRSKFWGRSIQ++PVGVL L
Sbjct: 481 YEADFPEKGIRFFSEKVSHHPTVIACHCEGKGWKFWGDTNLRSKFWGRSIQVEPVGVLTL 540

Query: 541 EFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQV 600
           EFDDGE FQWSKVT+ IYN+ILGK+YCDHHG M IRGNR+YSCTLKFKE SIL+RNPHQV
Sbjct: 541 EFDDGEVFQWSKVTSTIYNIILGKLYCDHHGVMQIRGNRQYSCTLKFKEQSILERNPHQV 600

Query: 601 HGFVEDGNGRKVATLFGKWDEGMYYVKGES-NGNPNRSSSSDGSMLWKSEKPPPNLTRYN 660
           +GFVED  G+K AT+FGKWD+ +YYV G+  N +     +S+ S+LWK  KPPPN+TRYN
Sbjct: 601 NGFVEDVAGQKAATIFGKWDDSLYYVAGDGINKSKVSDPASNASLLWKRTKPPPNVTRYN 660

Query: 661 LTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQD 720
           LTSFAITLNELT  L+E LPPTDSRLRPDQRHLENGEYEKAN EKQRLERRQR+SR+LQ+
Sbjct: 661 LTSFAITLNELTPGLQEILPPTDSRLRPDQRHLENGEYEKANLEKQRLERRQRMSRQLQE 719

Query: 721 NGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNENLVD 753
           +GW+PRWF R+GE   + +KY GGYWEAR  R WD C D+FGE  E L D
Sbjct: 721 SGWRPRWFERQGE--SDTFKYTGGYWEARGHRNWDDCPDIFGEFTEELAD 719

BLAST of Carg25162 vs. ExPASy Swiss-Prot
Match: Q9SU36 (Oxysterol-binding protein-related protein 2B OS=Arabidopsis thaliana OX=3702 GN=ORP2B PE=2 SV=2)

HSP 1 Score: 939.5 bits (2427), Expect = 2.4e-272
Identity = 471/737 (63.91%), Postives = 569/737 (77.20%), Query Frame = 0

Query: 32  LSRSRSLPVSFPAFSNANTQQISRSVDRVAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI 91
           L+RS+SLP +    S+  T Q  RS   VAG+LYKWTNYGKGWRSRWF+LRDG+LSYSKI
Sbjct: 3   LTRSKSLPATENGGSDRETLQSGRS---VAGILYKWTNYGKGWRSRWFLLRDGILSYSKI 62

Query: 92  ------SLLSQPNDDVQLIGKISTNRL---------SRRKQQKSGGIVHLKVSSFRESKS 151
                 +LLS P+DD++LIG IST+RL         SRRK  K+ GIVHLKVSS+RESKS
Sbjct: 63  RRPENVNLLS-PSDDLRLIGDISTDRLLRMKSCSGRSRRKHHKNIGIVHLKVSSYRESKS 122

Query: 152 DDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILNDNLPFV-PNDLSVSTQ 211
           D R+FYIFTATKTLHLRT+S+ DR  W+QALAS+R + PL+ +N +  FV P DLS+ST+
Sbjct: 123 DHRKFYIFTATKTLHLRTDSRSDRAAWLQALASTRGIVPLQSINGDFSFVSPKDLSISTE 182

Query: 212 KLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLIDTLREFEAANIEIEA 271
           +L+KRL EEG+++++VK+CEQI+ SEF E+Q Q+K+L E+R  L+D LR+ E AN+E EA
Sbjct: 183 RLKKRLFEEGMNESLVKECEQIVDSEFCEVQEQIKLLHEERKKLLDALRQLEMANLEAEA 242

Query: 272 NGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSDVDESFFYDTKESFTE 331
           +GIH+  YQL  H +S+   GKYSEC+T+ SSDD  KQ+ +D S+ DE+ F+DTKESF E
Sbjct: 243 SGIHDDVYQLRNHKYSSLGRGKYSECSTSASSDD--KQEFEDISEEDEASFHDTKESFGE 302

Query: 332 VTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQRRKKLPDPVEKEKRV 391
             +G                                   V ++ +RR KLPDP EKE+ V
Sbjct: 303 PDVGS----------------------------------VLTHFKRRTKLPDPAEKERGV 362

Query: 392 SLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVL 451
           SLWSMIKDNVGKDL RVCLPVYFNEPISSLQKC ED+EYS+LLDQAYE+G  G +LLR L
Sbjct: 363 SLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCFEDLEYSYLLDQAYEYGKSGKSLLRAL 422

Query: 452 KVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRG 511
            V+AFAVSGYAS+EGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHC G+G
Sbjct: 423 NVAAFAVSGYASTEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCEGKG 482

Query: 512 WKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGT 571
           WKFWG++NLRSKFWGRSIQL+PVG+L LEFDDGE FQWSKVTT IYN++LGK+YCDHHG 
Sbjct: 483 WKFWGDTNLRSKFWGRSIQLEPVGILTLEFDDGEIFQWSKVTTTIYNILLGKLYCDHHGI 542

Query: 572 MHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNG 631
           M IRGNR+YSC LKFKE SILDRNPHQV+GFVED  G+K AT+FGKW++ +YYV G+   
Sbjct: 543 MKIRGNRQYSCMLKFKEQSILDRNPHQVNGFVEDVTGKKAATVFGKWNDSLYYVAGDG-- 602

Query: 632 NPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHL 691
                + +  S+LWK+ K PPN+TRYN TSFA+TLNEL   L+E+LPPTDSRLRPDQRHL
Sbjct: 603 ----INKASASLLWKATKAPPNVTRYNFTSFAMTLNELIPGLEEKLPPTDSRLRPDQRHL 662

Query: 692 ENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERK 751
           ENGEYEKAN EKQRLERRQR+SR++Q++GW+PRWF  +GE   E YKY GGYWEAR  + 
Sbjct: 663 ENGEYEKANEEKQRLERRQRMSRQIQESGWRPRWFEPQGE--SESYKYTGGYWEARDVKS 691

Query: 752 WDGCLDVFGELNENLVD 753
           WD C ++FGE  E + D
Sbjct: 723 WDDCPNIFGEFTEEVAD 691

BLAST of Carg25162 vs. ExPASy Swiss-Prot
Match: Q8L751 (Oxysterol-binding protein-related protein 1C OS=Arabidopsis thaliana OX=3702 GN=ORP1C PE=2 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 8.5e-230
Identity = 406/720 (56.39%), Postives = 518/720 (71.94%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSY------SKISLLSQPNDDVQLIGKISTNRLS 119
           +AG+LYKW NYG+GWR RWFVL+DGVLSY       KI +  +     ++IG  S   +S
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYKIHGPDKIFVSPETEKGSKVIGDESARMIS 165

Query: 120 RRK-----------QQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVI 179
           R             ++K  G VHLKVSS RES+SDD+RF IFT TK LHLR E+++DR  
Sbjct: 166 RHNRRGGSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTT 225

Query: 180 WIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEF 239
           W++AL + + +FP R+ N  L    N+L++ST+K+  RL+EEG+S+  ++DCEQI+ SEF
Sbjct: 226 WVEALQAVKDMFP-RMSNSELMAPTNNLAMSTEKIRLRLIEEGVSELAIQDCEQIMKSEF 285

Query: 240 SEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSG-----K 299
           S +Q QL +L +++  LIDTLR+ E   +++E   +   + Q         CSG     K
Sbjct: 286 SALQSQLVLLKQKQWLLIDTLRQLETEKVDLENTVVDESQRQA-----DNGCSGELRHEK 345

Query: 300 YSECATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVR 359
           +SE   TES DD E+ D  ++  D +E+ F+DT++      L   S K +     T    
Sbjct: 346 FSEGTATESDDDNERGDAAEEEFDEEENTFFDTRD-----FLSSSSFKSSGSGFRTSSF- 405

Query: 360 DSRQDNAEKMHDEQIENFVHS------NVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLA 419
            S +D  E   ++ I+  + S       V+RRK LPDPVEKEK VSLWSMIKDN+GKDL 
Sbjct: 406 SSDEDGFES--EDDIDPSIKSIGCNYPRVKRRKNLPDPVEKEKSVSLWSMIKDNIGKDLT 465

Query: 420 RVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEG 479
           +VCLPVYFNEP+SSLQKC ED+EYS+LLD+A+E+G +GN+L+R+L V+AFAVSGYAS+EG
Sbjct: 466 KVCLPVYFNEPLSSLQKCFEDLEYSYLLDRAFEYGKRGNSLMRILNVAAFAVSGYASTEG 525

Query: 480 RHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWG 539
           R CKPFNPLLGETYEAD+P+KG+RFFSEKVSHHP V+ACHC G GWKFWG+SNLRSKFWG
Sbjct: 526 RICKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMVVACHCDGTGWKFWGDSNLRSKFWG 585

Query: 540 RSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKF 599
           RSIQLDPVGVL L+FDDGE  QWSKVTT+IYNLILGK+YCDH+GTM I G+ EYSC LKF
Sbjct: 586 RSIQLDPVGVLTLQFDDGEILQWSKVTTSIYNLILGKLYCDHYGTMRIEGSAEYSCKLKF 645

Query: 600 KEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNGNPNRSSSSDGS-MLW 659
           KE SI+DRNPHQVHG V++ +G+ VAT+FGKWDE +++V G+ +G    S    G+ +LW
Sbjct: 646 KEQSIIDRNPHQVHGIVQNKSGKTVATMFGKWDESIHFVTGDCSGKGKLSEDMSGAQLLW 705

Query: 660 KSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQR 719
           K  KPP N T+YNLT FAITLNELT  LKERLPPTDSRLRPDQR+LENGE+E AN EK R
Sbjct: 706 KRSKPPGNATKYNLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKLR 765

Query: 720 LERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNEN 750
           LE+RQR +RK+Q+ GWKPRWF +  E   E Y+Y GGYWEAR++  W  C D+FG ++ +
Sbjct: 766 LEQRQRQARKMQERGWKPRWFMK--EKGSESYRYKGGYWEAREDGSWVDCPDIFGHIDSD 809

BLAST of Carg25162 vs. ExPASy Swiss-Prot
Match: O80866 (Oxysterol-binding protein-related protein 1A OS=Arabidopsis thaliana OX=3702 GN=ORP1A PE=2 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 3.5e-223
Identity = 397/710 (55.92%), Postives = 505/710 (71.13%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSY------SKISLLSQPNDDVQLIGKISTNRLS 119
           +AG+LYKW NYGKGWR RWFVL+DGVLSY       KI ++ +     ++IG+ ST  +S
Sbjct: 73  IAGILYKWVNYGKGWRPRWFVLQDGVLSYYKIKGPDKIVVIHETEKGSRVIGEESTRMIS 132

Query: 120 RRK------------QQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRV 179
           R K            ++K  G VHLKVSS RES+SDD+RF IFT TK LHLR E+++DR 
Sbjct: 133 RNKRHAATNNTNHQLRRKPFGEVHLKVSSIRESRSDDKRFSIFTGTKRLHLRAETREDRE 192

Query: 180 IWIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSE 239
            WI+AL + + +FP R+ N  L    N+L +S +KL  RL+EEG+S++ ++DCEQI  SE
Sbjct: 193 AWIEALQAVKDMFP-RMSNCELMAPTNNLDISIEKLRLRLVEEGVSESAIQDCEQITRSE 252

Query: 240 FSEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSEC 299
           FS +Q QL +L +++  LIDTLR+ E   +++E   +   + Q    D         SE 
Sbjct: 253 FSAIQSQLLLLKQKQWLLIDTLRQLETEKVDLENTVVDETQRQAGNGD---------SEE 312

Query: 300 ATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQ 359
             +ES DD E+ D  ++  D  +S    +  SF  +     +   +SD        +S  
Sbjct: 313 TISESDDDNEQFDEAEEEMDTCDSL---SSSSFKSIGSVFRTSSFSSDDDGLTNGFESEN 372

Query: 360 DNAEKMHDEQIENFVHSNVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNE 419
           D+ +     +   F + +V+RRKKLPDPVEKEK VSLWSMIKDN+GKDL +VCLPVYFNE
Sbjct: 373 DDVDP--SIKTIGFNYPHVKRRKKLPDPVEKEKSVSLWSMIKDNIGKDLTKVCLPVYFNE 432

Query: 420 PISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLL 479
           P+SSLQKC ED+EYS+LLDQA E G +GNNL+R+L V+AFAVSGYAS+EGR CKPFNP+L
Sbjct: 433 PLSSLQKCFEDLEYSYLLDQASEWGKRGNNLMRILNVAAFAVSGYASTEGRICKPFNPML 492

Query: 480 GETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGV 539
           GETYEAD+P+KG+RFFSEKVSHHP ++ACHC G GWKFWG+SNL+SKFWGRSIQLDP+G+
Sbjct: 493 GETYEADYPDKGLRFFSEKVSHHPMIVACHCDGTGWKFWGDSNLKSKFWGRSIQLDPIGL 552

Query: 540 LNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNP 599
           L L+FDDGE  QWSKVTT+IYNLILGK+YCDH+GTM I GN EYSC LKFKE S++DRNP
Sbjct: 553 LTLQFDDGEIVQWSKVTTSIYNLILGKLYCDHYGTMKIEGNGEYSCKLKFKEQSMIDRNP 612

Query: 600 HQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTR 659
           HQV G VED NG+ VA LFGKWDE ++YV  +  G  N S      +LWK  K P N T+
Sbjct: 613 HQVQGIVEDKNGKTVARLFGKWDESIHYVMVD-QGKVNES-----HLLWKRNKQPENPTK 672

Query: 660 YNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKL 719
           YNLT F ITLNELT  LKE+LPPTDSRLRPDQR+LE GEYE  NAEK RLE+RQR +R++
Sbjct: 673 YNLTRFGITLNELTPGLKEKLPPTDSRLRPDQRYLEKGEYEMGNAEKLRLEQRQRQAREM 732

Query: 720 QDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNENL 751
           Q+ GWKP+WF +  E   E Y+Y+GGYWEAR    WD C D+FG++++++
Sbjct: 733 QERGWKPKWFRK--EKGSETYRYIGGYWEARDSGSWDDCPDIFGQVHQSI 759

BLAST of Carg25162 vs. ExPASy Swiss-Prot
Match: Q9SAF0 (Oxysterol-binding protein-related protein 1D OS=Arabidopsis thaliana OX=3702 GN=ORP1D PE=2 SV=1)

HSP 1 Score: 735.3 bits (1897), Expect = 6.8e-211
Identity = 388/722 (53.74%), Postives = 498/722 (68.98%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI----SLLSQPNDD--VQLIGKI------ 119
           VAG++YKW NYGKGWR+RWF L DGVLSY KI     ++  P+ +  V++IG+       
Sbjct: 95  VAGIMYKWVNYGKGWRARWFELEDGVLSYYKIHGPDKIVMNPSREKGVRVIGEESVRYIR 154

Query: 120 -----STNRL-----SRRKQQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESK 179
                S+NRL     +  +  K  G +HLKVSS R SKSDD+R  IFT TKTLHLR  SK
Sbjct: 155 KASCGSSNRLGASAVAASRPCKPFGEIHLKVSSIRASKSDDKRLAIFTGTKTLHLRCVSK 214

Query: 180 KDRVIWIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQI 239
           ++R  W++A   ++ LFP     D LP    D  VST+KL ++LL+EG+ + VVKDCE I
Sbjct: 215 ENRAAWVEAFQVAKDLFPRVASGDILP--SEDAVVSTEKLREKLLQEGVGETVVKDCEAI 274

Query: 240 ILSEFSEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGK 299
           +LSE S +Q +LKVL  +   L+DTLR+ E   IE+EA  +     +  +HD    C G+
Sbjct: 275 MLSEVSVLQNRLKVLTHKHIILLDTLRQLETEKIELEATVVD----ETKEHD---SCCGQ 334

Query: 300 ----------YSECATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGA 359
                      SE + ++S  D E QD  D  SD D+  ++DT +      L   +++ A
Sbjct: 335 GRRFSDFYSVMSEVSASDSEADNESQDGADVESDEDDVPYFDTND-----ILSAEAMRSA 394

Query: 360 SDSSDTLKVRDSRQDNAEKMHDE-QIENFV--HSNVQRRKKLPDPVEKEKRVSLWSMIKD 419
           S  S   +   S  D      D  QI   +  +  V+RR  LP+P EKEK V LWS+IK+
Sbjct: 395 SYRSREAEGNGSIYDKDPFFSDRLQIPARIPQYPYVRRRDNLPEPKEKEKPVGLWSIIKE 454

Query: 420 NVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVS 479
           N+GKDL+ VCLPVYFNEP+SSLQKC ED+EYS+L+D+A E G QGN L+R+L ++AFAVS
Sbjct: 455 NIGKDLSGVCLPVYFNEPLSSLQKCFEDLEYSYLIDRALEWGKQGNELMRILNIAAFAVS 514

Query: 480 GYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESN 539
           GYAS+EGR CKPFNPLLGETYEAD+P+KG+RFFSEKVSHHP ++ACHC G+GW FWG+SN
Sbjct: 515 GYASTEGRQCKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMIVACHCEGQGWNFWGDSN 574

Query: 540 LRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNRE 599
           ++ KFWGRSIQLDPVGVL L+FDDGE +QWSKVTT+IYN+ILGK+YCDH+GTM I+G   
Sbjct: 575 IKGKFWGRSIQLDPVGVLTLKFDDGEIYQWSKVTTSIYNIILGKLYCDHYGTMRIKGGSN 634

Query: 600 YSCTLKFKEPSILDRNPHQVHGFVEDG-NGRKVATLFGKWDEGMYYVKGESNGNP-NRSS 659
           YSC LKFKE S++DRNP QVHGFV+D   G KVA L GKWDE MYYV G+    P     
Sbjct: 635 YSCRLKFKEQSVIDRNPRQVHGFVQDNRTGEKVAILIGKWDEAMYYVLGDPTTKPKGYDP 694

Query: 660 SSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYE 719
            ++  +LW+ +K P   TRYNL+ FAI+LNE+T  + ++LPPTDSRLRPDQRHLENGEYE
Sbjct: 695 MTEAVLLWERDKSPTK-TRYNLSPFAISLNEITPGMIDKLPPTDSRLRPDQRHLENGEYE 754

Query: 720 KANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLD 744
            ANAEK RLE+ QR +R+LQ+ GWKPRWF +   D +  Y+Y+GGYWEAR+++ WD   D
Sbjct: 755 SANAEKLRLEQLQRQARRLQEKGWKPRWFEK---DEEGNYRYLGGYWEAREKKDWDRITD 798

BLAST of Carg25162 vs. ExPASy TrEMBL
Match: A0A6J1FW34 (oxysterol-binding protein-related protein 2A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447847 PE=4 SV=1)

HSP 1 Score: 1534.6 bits (3972), Expect = 0.0e+00
Identity = 751/756 (99.34%), Postives = 754/756 (99.74%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVS QKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI
Sbjct: 181 DNLPFVPNDLSVSAQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIE+EANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIELEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDT ESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTNESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 756

BLAST of Carg25162 vs. ExPASy TrEMBL
Match: A0A6J1KX27 (oxysterol-binding protein-related protein 2A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111498345 PE=4 SV=1)

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 740/756 (97.88%), Postives = 747/756 (98.81%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSN +TQQISRSVDRV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNTSTQQISRSVDRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRK+QKS
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKRQKS 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN
Sbjct: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI
Sbjct: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD
Sbjct: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360
           VDESFFYDT ESFTE TLG GSVKGA DSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ
Sbjct: 301 VDESFFYDTNESFTEFTLGYGSVKGAFDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQ 360

Query: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420
           RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ
Sbjct: 361 RRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQ 420

Query: 421 AYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480
           AYEHGIQGNNLLRVLKV++FAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV
Sbjct: 421 AYEHGIQGNNLLRVLKVASFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKV 480

Query: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540
           SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI
Sbjct: 481 SHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNI 540

Query: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFG 600
           YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGF+EDGNGRKVATLFG
Sbjct: 541 YNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDGNGRKVATLFG 600

Query: 601 KWDEGMYYVKGESNGNPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660
           KWDEGMYYV+GESNGNPNRSSSSD SMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER
Sbjct: 601 KWDEGMYYVEGESNGNPNRSSSSDVSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKER 660

Query: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEP 720
           LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGED DE 
Sbjct: 661 LPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDTDEA 720

Query: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
           YKYMGGYWEARKERKWDGCLDVFGELNENLV +SGS
Sbjct: 721 YKYMGGYWEARKERKWDGCLDVFGELNENLVGDSGS 756

BLAST of Carg25162 vs. ExPASy TrEMBL
Match: A0A6J1E933 (oxysterol-binding protein-related protein 2A-like OS=Cucurbita moschata OX=3662 GN=LOC111431016 PE=4 SV=1)

HSP 1 Score: 1357.4 bits (3512), Expect = 0.0e+00
Identity = 669/758 (88.26%), Postives = 706/758 (93.14%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQQISRSVDRV 60
           MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVS PAFSN N+ Q+S SV RV
Sbjct: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSLPAFSNNNSLQVSGSVTRV 60

Query: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQKS 120
           AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI LLS P+DDV+LIG+ISTNRL R K+QK+
Sbjct: 61  AGVLYKWTNYGKGWRSRWFVLRDGVLSYSKIGLLS-PDDDVRLIGQISTNRLPRGKRQKT 120

Query: 121 GGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILN 180
           GGIVHLKVS FRESKSD+RRFYIFTATKTLHLRTESK DRV WIQALASSR+LFPL+I+N
Sbjct: 121 GGIVHLKVSRFRESKSDERRFYIFTATKTLHLRTESKNDRVTWIQALASSRNLFPLKIVN 180

Query: 181 DNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLI 240
           DNLPFVPNDLS+S QKL+KRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLE+RSCLI
Sbjct: 181 DNLPFVPNDLSLSAQKLKKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEERSCLI 240

Query: 241 DTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSD 300
           DTLRE EAANIEIEANGIHNGEYQLMKHDFSTECSGKYSEC TTESS+DVEKQDL+DGSD
Sbjct: 241 DTLRELEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECTTTESSEDVEKQDLEDGSD 300

Query: 301 VDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQ-IENFVHSNV 360
           VDE+FFYDT ESF+E+TL CGSV+G SDS D +K   SRQD+ EKMH +Q IEN VH NV
Sbjct: 301 VDENFFYDTNESFSELTLSCGSVEGGSDSLDKVKELRSRQDDVEKMHHKQSIENSVHVNV 360

Query: 361 QRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLD 420
           +RRK LPDPVEKEKRVSLWSMIKDNVGKDL RVCLPVYFNEPISSLQKCCED+EYSHLLD
Sbjct: 361 ERRKNLPDPVEKEKRVSLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCCEDLEYSHLLD 420

Query: 421 QAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEK 480
           QAYE+G QGNN LRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEK
Sbjct: 421 QAYEYGRQGNNFLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEK 480

Query: 481 VSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTN 540
           VSHHPTVIACHC GRGW+FWG+SNLRSKFWGRSIQLDPVGVLNLEFDDGE FQWSKVTTN
Sbjct: 481 VSHHPTVIACHCEGRGWRFWGDSNLRSKFWGRSIQLDPVGVLNLEFDDGELFQWSKVTTN 540

Query: 541 IYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLF 600
           IYNLILGKVYCDHHGTM IRGNREYSCTLKFKEPSILDRNPHQVHGFVED NGRKVATLF
Sbjct: 541 IYNLILGKVYCDHHGTMQIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDANGRKVATLF 600

Query: 601 GKWDEGMYYVKGESNGNPN-RSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLK 660
           GKWDEGMYYV+GES+ NP     SSD SMLWKSEKPPPNLTRYNLTSFAITLNELT DLK
Sbjct: 601 GKWDEGMYYVEGESSVNPKCNMPSSDESMLWKSEKPPPNLTRYNLTSFAITLNELTLDLK 660

Query: 661 ERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDAD 720
           ERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQD+GWKPRWF++EGED  
Sbjct: 661 ERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDSGWKPRWFYKEGEDG- 720

Query: 721 EPYKYMGGYWEARKERKWDGCLDVFGELNENLVDESGS 757
            PY+Y GGYWEARKE KWDGC DVF ELNE++V ESGS
Sbjct: 721 -PYRYTGGYWEARKEGKWDGCPDVFSELNESMVGESGS 755

BLAST of Carg25162 vs. ExPASy TrEMBL
Match: A0A5D3D7I6 (Oxysterol-binding protein-related protein 2A isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001590 PE=4 SV=1)

HSP 1 Score: 1355.9 bits (3508), Expect = 0.0e+00
Identity = 666/756 (88.10%), Postives = 706/756 (93.39%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQ-QISRSVDR 60
           MRVKEMHPLCCI+LENFSGGIGDQSPEEA+SLSRSRSLPVS PAFSN N   QIS S+ R
Sbjct: 1   MRVKEMHPLCCITLENFSGGIGDQSPEEATSLSRSRSLPVSIPAFSNNNNPLQISGSITR 60

Query: 61  VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQK 120
           VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISL+S  +DDV+LIG+ISTNRLSRRK+QK
Sbjct: 61  VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLISPGDDDVRLIGEISTNRLSRRKRQK 120

Query: 121 SGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRIL 180
           +GGIVHLKVS FRESKSDDRRFYIFTATKTLHLRTESKKDRV WIQALASSRSLFPL+IL
Sbjct: 121 TGGIVHLKVSRFRESKSDDRRFYIFTATKTLHLRTESKKDRVTWIQALASSRSLFPLQIL 180

Query: 181 NDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCL 240
           NDNLPFVPNDLSVSTQKL+KRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLE+RSCL
Sbjct: 181 NDNLPFVPNDLSVSTQKLKKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEERSCL 240

Query: 241 IDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGS 300
           IDTLRE EAANIEIEANG+HNGEYQLMKHDFSTECSGKYSEC TTESSDDVEKQD++DGS
Sbjct: 241 IDTLRELEAANIEIEANGVHNGEYQLMKHDFSTECSGKYSECTTTESSDDVEKQDIEDGS 300

Query: 301 DVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQ-IENFVHSN 360
           D DESFFYDTKE+F+E+TL CGSV+G SDS D +K  +SR+D+ EKMH +Q IEN VH +
Sbjct: 301 DADESFFYDTKETFSELTLSCGSVEGTSDSLDKVKDLESRKDDVEKMHHKQRIENSVHFS 360

Query: 361 VQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLL 420
           VQRRKKLPDP+EKEKRVSLWSMIKDNVGKDL RVCLPVYFNEPISSLQKCCEDMEYSHLL
Sbjct: 361 VQRRKKLPDPIEKEKRVSLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCCEDMEYSHLL 420

Query: 421 DQAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE 480
           DQAYEHG QGNN LRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE
Sbjct: 421 DQAYEHGRQGNNFLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE 480

Query: 481 KVSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTT 540
           KVSHHPTVIACHC GRGW+FWG+SNLRSKFWGRSIQLDPVGVLNLEFDDGE FQWSKVTT
Sbjct: 481 KVSHHPTVIACHCEGRGWRFWGDSNLRSKFWGRSIQLDPVGVLNLEFDDGEVFQWSKVTT 540

Query: 541 NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATL 600
           NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGF+ED +GRKVAT+
Sbjct: 541 NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDASGRKVATI 600

Query: 601 FGKWDEGMYYVKGESNGNPNRSS-SSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADL 660
           FGKWDEGMYY++GE NGN  R+S  SD  MLWKSEKP P+LTRYNLTSFAITLNELT DL
Sbjct: 601 FGKWDEGMYYMEGEINGNRKRNSPPSDEFMLWKSEKPAPDLTRYNLTSFAITLNELTPDL 660

Query: 661 KERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDA 720
           KERLPPTDSRLRPDQRHLENGEYEKAN EKQRLERRQRISRKLQDNGWKPRWF REGED 
Sbjct: 661 KERLPPTDSRLRPDQRHLENGEYEKANEEKQRLERRQRISRKLQDNGWKPRWFCREGEDG 720

Query: 721 DEPYKYMGGYWEARKERKWDGCLDVFGELNENLVDE 754
             PY+YMGGYWEAR+E  WD C DVFGELNE++V E
Sbjct: 721 --PYRYMGGYWEAREEGNWDNCPDVFGELNESMVGE 754

BLAST of Carg25162 vs. ExPASy TrEMBL
Match: A0A1S3BKQ1 (oxysterol-binding protein-related protein 2A isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490906 PE=4 SV=1)

HSP 1 Score: 1354.7 bits (3505), Expect = 0.0e+00
Identity = 665/756 (87.96%), Postives = 706/756 (93.39%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEEASSLSRSRSLPVSFPAFSNANTQ-QISRSVDR 60
           MRVKEMHPLCCI+LENFSGGIGDQSPEEA+SLSRSRSLPVS PAFSN N   QIS S+ R
Sbjct: 1   MRVKEMHPLCCITLENFSGGIGDQSPEEATSLSRSRSLPVSIPAFSNNNNPLQISGSITR 60

Query: 61  VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLLSQPNDDVQLIGKISTNRLSRRKQQK 120
           VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISL+S  +DDV+LIG+ISTNRLSRRK+QK
Sbjct: 61  VAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKISLISPGDDDVRLIGEISTNRLSRRKRQK 120

Query: 121 SGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRIL 180
           +GGIVHLKVS FRESKSDDRRFYIFTATKTLHLRTESKKDRV WIQALASSRSLFPL+IL
Sbjct: 121 TGGIVHLKVSRFRESKSDDRRFYIFTATKTLHLRTESKKDRVTWIQALASSRSLFPLQIL 180

Query: 181 NDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCL 240
           NDNLPFVPNDLS+STQKL+KRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLE+RSCL
Sbjct: 181 NDNLPFVPNDLSLSTQKLKKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEERSCL 240

Query: 241 IDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGS 300
           IDTLRE EAANIEIEANG+HNGEYQLMKHDFSTECSGKYSEC TTESSDDVEKQD++DGS
Sbjct: 241 IDTLRELEAANIEIEANGVHNGEYQLMKHDFSTECSGKYSECTTTESSDDVEKQDIEDGS 300

Query: 301 DVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQ-IENFVHSN 360
           D DESFFYDTKE+F+E+TL CGSV+G SDS D +K  +SR+D+ EKMH +Q IEN VH +
Sbjct: 301 DADESFFYDTKETFSELTLSCGSVEGTSDSLDKVKDLESRKDDVEKMHHKQRIENSVHFS 360

Query: 361 VQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLL 420
           VQRRKKLPDP+EKEKRVSLWSMIKDNVGKDL RVCLPVYFNEPISSLQKCCEDMEYSHLL
Sbjct: 361 VQRRKKLPDPIEKEKRVSLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCCEDMEYSHLL 420

Query: 421 DQAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE 480
           DQAYEHG QGNN LRVLKV+AFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE
Sbjct: 421 DQAYEHGRQGNNFLRVLKVAAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSE 480

Query: 481 KVSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTT 540
           KVSHHPTVIACHC GRGW+FWG+SNLRSKFWGRSIQLDPVGVLNLEFDDGE FQWSKVTT
Sbjct: 481 KVSHHPTVIACHCEGRGWRFWGDSNLRSKFWGRSIQLDPVGVLNLEFDDGEVFQWSKVTT 540

Query: 541 NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATL 600
           NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGF+ED +GRKVAT+
Sbjct: 541 NIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQVHGFIEDASGRKVATI 600

Query: 601 FGKWDEGMYYVKGESNGNPNRSS-SSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADL 660
           FGKWDEGMYY++GE NGN  R+S  SD  MLWKSEKP P+LTRYNLTSFAITLNELT DL
Sbjct: 601 FGKWDEGMYYMEGEINGNRKRNSPPSDEFMLWKSEKPAPDLTRYNLTSFAITLNELTPDL 660

Query: 661 KERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDA 720
           KERLPPTDSRLRPDQRHLENGEYEKAN EKQRLERRQRISRKLQDNGWKPRWF REGED 
Sbjct: 661 KERLPPTDSRLRPDQRHLENGEYEKANEEKQRLERRQRISRKLQDNGWKPRWFCREGEDG 720

Query: 721 DEPYKYMGGYWEARKERKWDGCLDVFGELNENLVDE 754
             PY+YMGGYWEAR+E  WD C DVFGELNE++V E
Sbjct: 721 --PYRYMGGYWEAREEGNWDNCPDVFGELNESMVGE 754

BLAST of Carg25162 vs. TAIR 10
Match: AT4G22540.1 (OSBP(oxysterol binding protein)-related protein 2A )

HSP 1 Score: 985.7 bits (2547), Expect = 2.0e-287
Identity = 506/770 (65.71%), Postives = 597/770 (77.53%), Query Frame = 0

Query: 1   MRVKEMHPLCCISLENFSGGIGDQSPEE--ASSLSRSRSLPV-SFPAFSNANTQQISRSV 60
           MRVKE+HPLCCI+LE+   GI  QSPEE   ++L+RSRSLP  S    S + T       
Sbjct: 1   MRVKELHPLCCITLES-PHGINSQSPEEPVTTALTRSRSLPAKSLTGGSESET------- 60

Query: 61  DRVAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI------SLLSQPNDDVQLIGKISTNR 120
             VAG+LYKWTN+GKGWRSRWF+LR+G+LSYSKI      +LLS P+DDV+LIG IS  R
Sbjct: 61  --VAGILYKWTNFGKGWRSRWFLLRNGILSYSKIRRPENLNLLS-PDDDVRLIGDISGER 120

Query: 121 LS-------RRKQQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVIWI 180
           LS       RRKQQK+ GIVHLKVSSFRESKSDDR+FYIFTATKTLHLRT+S  DR  W+
Sbjct: 121 LSRMDSCSGRRKQQKTVGIVHLKVSSFRESKSDDRKFYIFTATKTLHLRTDSITDRAAWL 180

Query: 181 QALASSRSLFPLRILNDNLPFV-PNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEFS 240
           QALAS++ +FPLR LN +  F+ P DLS+ST++L+KRL EEG+++ +VK+CEQI+LSEFS
Sbjct: 181 QALASTKCIFPLRSLNGDFSFIPPKDLSISTERLKKRLHEEGMNENLVKECEQIMLSEFS 240

Query: 241 EMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSGKYSECAT 300
           EM GQ+K+L E+R+ L+D LR+ EAAN+E+ A+G         KH+FS+   GKYSEC+T
Sbjct: 241 EMHGQVKLLHEERTNLLDALRQLEAANLEVGASG---------KHEFSSLGRGKYSECST 300

Query: 301 TESSDDVEKQDLDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVRDSRQDNA 360
           T SSD   KQ+ +D S+ DE  F+DTKE F E  +G  S    S                
Sbjct: 301 TASSDG--KQEFEDVSEEDEPSFHDTKEFFNEPNIGSESNLAISG--------------- 360

Query: 361 EKMHDEQIENFVHSNVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLARVCLPVYFNEPIS 420
                       H++++RR KLPDP EKEK VSLWSMIKDNVGKDL RVCLPVYFNEPIS
Sbjct: 361 ------------HTDIKRRTKLPDPAEKEKGVSLWSMIKDNVGKDLTRVCLPVYFNEPIS 420

Query: 421 SLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEGRHCKPFNPLLGET 480
           SLQKC ED+EYS+LLD+AYEHG  GN LLR L V+AFAVSGYAS+EGRHCKPFNPLLGET
Sbjct: 421 SLQKCFEDLEYSYLLDRAYEHGKSGNGLLRALNVAAFAVSGYASTEGRHCKPFNPLLGET 480

Query: 481 YEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWGRSIQLDPVGVLNL 540
           YEADFPEKGIRFFSEKVSHHPTVIACHC G+GWKFWG++NLRSKFWGRSIQ++PVGVL L
Sbjct: 481 YEADFPEKGIRFFSEKVSHHPTVIACHCEGKGWKFWGDTNLRSKFWGRSIQVEPVGVLTL 540

Query: 541 EFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKFKEPSILDRNPHQV 600
           EFDDGE FQWSKVT+ IYN+ILGK+YCDHHG M IRGNR+YSCTLKFKE SIL+RNPHQV
Sbjct: 541 EFDDGEVFQWSKVTSTIYNIILGKLYCDHHGVMQIRGNRQYSCTLKFKEQSILERNPHQV 600

Query: 601 HGFVEDGNGRKVATLFGKWDEGMYYVKGES-NGNPNRSSSSDGSMLWKSEKPPPNLTRYN 660
           +GFVED  G+K AT+FGKWD+ +YYV G+  N +     +S+ S+LWK  KPPPN+TRYN
Sbjct: 601 NGFVEDVAGQKAATIFGKWDDSLYYVAGDGINKSKVSDPASNASLLWKRTKPPPNVTRYN 660

Query: 661 LTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQRLERRQRISRKLQD 720
           LTSFAITLNELT  L+E LPPTDSRLRPDQRHLENGEYEKAN EKQRLERRQR+SR+LQ+
Sbjct: 661 LTSFAITLNELTPGLQEILPPTDSRLRPDQRHLENGEYEKANLEKQRLERRQRMSRQLQE 719

Query: 721 NGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNENLVD 753
           +GW+PRWF R+GE   + +KY GGYWEAR  R WD C D+FGE  E L D
Sbjct: 721 SGWRPRWFERQGE--SDTFKYTGGYWEARGHRNWDDCPDIFGEFTEELAD 719

BLAST of Carg25162 vs. TAIR 10
Match: AT4G12460.1 (OSBP(oxysterol binding protein)-related protein 2B )

HSP 1 Score: 939.5 bits (2427), Expect = 1.7e-273
Identity = 471/737 (63.91%), Postives = 569/737 (77.20%), Query Frame = 0

Query: 32  LSRSRSLPVSFPAFSNANTQQISRSVDRVAGVLYKWTNYGKGWRSRWFVLRDGVLSYSKI 91
           L+RS+SLP +    S+  T Q  RS   VAG+LYKWTNYGKGWRSRWF+LRDG+LSYSKI
Sbjct: 3   LTRSKSLPATENGGSDRETLQSGRS---VAGILYKWTNYGKGWRSRWFLLRDGILSYSKI 62

Query: 92  ------SLLSQPNDDVQLIGKISTNRL---------SRRKQQKSGGIVHLKVSSFRESKS 151
                 +LLS P+DD++LIG IST+RL         SRRK  K+ GIVHLKVSS+RESKS
Sbjct: 63  RRPENVNLLS-PSDDLRLIGDISTDRLLRMKSCSGRSRRKHHKNIGIVHLKVSSYRESKS 122

Query: 152 DDRRFYIFTATKTLHLRTESKKDRVIWIQALASSRSLFPLRILNDNLPFV-PNDLSVSTQ 211
           D R+FYIFTATKTLHLRT+S+ DR  W+QALAS+R + PL+ +N +  FV P DLS+ST+
Sbjct: 123 DHRKFYIFTATKTLHLRTDSRSDRAAWLQALASTRGIVPLQSINGDFSFVSPKDLSISTE 182

Query: 212 KLEKRLLEEGISDAVVKDCEQIILSEFSEMQGQLKVLLEQRSCLIDTLREFEAANIEIEA 271
           +L+KRL EEG+++++VK+CEQI+ SEF E+Q Q+K+L E+R  L+D LR+ E AN+E EA
Sbjct: 183 RLKKRLFEEGMNESLVKECEQIVDSEFCEVQEQIKLLHEERKKLLDALRQLEMANLEAEA 242

Query: 272 NGIHNGEYQLMKHDFSTECSGKYSECATTESSDDVEKQDLDDGSDVDESFFYDTKESFTE 331
           +GIH+  YQL  H +S+   GKYSEC+T+ SSDD  KQ+ +D S+ DE+ F+DTKESF E
Sbjct: 243 SGIHDDVYQLRNHKYSSLGRGKYSECSTSASSDD--KQEFEDISEEDEASFHDTKESFGE 302

Query: 332 VTLGCGSVKGASDSSDTLKVRDSRQDNAEKMHDEQIENFVHSNVQRRKKLPDPVEKEKRV 391
             +G                                   V ++ +RR KLPDP EKE+ V
Sbjct: 303 PDVGS----------------------------------VLTHFKRRTKLPDPAEKERGV 362

Query: 392 SLWSMIKDNVGKDLARVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVL 451
           SLWSMIKDNVGKDL RVCLPVYFNEPISSLQKC ED+EYS+LLDQAYE+G  G +LLR L
Sbjct: 363 SLWSMIKDNVGKDLTRVCLPVYFNEPISSLQKCFEDLEYSYLLDQAYEYGKSGKSLLRAL 422

Query: 452 KVSAFAVSGYASSEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRG 511
            V+AFAVSGYAS+EGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHC G+G
Sbjct: 423 NVAAFAVSGYASTEGRHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCEGKG 482

Query: 512 WKFWGESNLRSKFWGRSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGT 571
           WKFWG++NLRSKFWGRSIQL+PVG+L LEFDDGE FQWSKVTT IYN++LGK+YCDHHG 
Sbjct: 483 WKFWGDTNLRSKFWGRSIQLEPVGILTLEFDDGEIFQWSKVTTTIYNILLGKLYCDHHGI 542

Query: 572 MHIRGNREYSCTLKFKEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNG 631
           M IRGNR+YSC LKFKE SILDRNPHQV+GFVED  G+K AT+FGKW++ +YYV G+   
Sbjct: 543 MKIRGNRQYSCMLKFKEQSILDRNPHQVNGFVEDVTGKKAATVFGKWNDSLYYVAGDG-- 602

Query: 632 NPNRSSSSDGSMLWKSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHL 691
                + +  S+LWK+ K PPN+TRYN TSFA+TLNEL   L+E+LPPTDSRLRPDQRHL
Sbjct: 603 ----INKASASLLWKATKAPPNVTRYNFTSFAMTLNELIPGLEEKLPPTDSRLRPDQRHL 662

Query: 692 ENGEYEKANAEKQRLERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERK 751
           ENGEYEKAN EKQRLERRQR+SR++Q++GW+PRWF  +GE   E YKY GGYWEAR  + 
Sbjct: 663 ENGEYEKANEEKQRLERRQRMSRQIQESGWRPRWFEPQGE--SESYKYTGGYWEARDVKS 691

Query: 752 WDGCLDVFGELNENLVD 753
           WD C ++FGE  E + D
Sbjct: 723 WDDCPNIFGEFTEEVAD 691

BLAST of Carg25162 vs. TAIR 10
Match: AT4G08180.1 (OSBP(oxysterol binding protein)-related protein 1C )

HSP 1 Score: 798.1 bits (2060), Expect = 6.0e-231
Identity = 406/720 (56.39%), Postives = 518/720 (71.94%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSY------SKISLLSQPNDDVQLIGKISTNRLS 119
           +AG+LYKW NYG+GWR RWFVL+DGVLSY       KI +  +     ++IG  S   +S
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYKIHGPDKIFVSPETEKGSKVIGDESARMIS 165

Query: 120 RRK-----------QQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVI 179
           R             ++K  G VHLKVSS RES+SDD+RF IFT TK LHLR E+++DR  
Sbjct: 166 RHNRRGGSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTT 225

Query: 180 WIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEF 239
           W++AL + + +FP R+ N  L    N+L++ST+K+  RL+EEG+S+  ++DCEQI+ SEF
Sbjct: 226 WVEALQAVKDMFP-RMSNSELMAPTNNLAMSTEKIRLRLIEEGVSELAIQDCEQIMKSEF 285

Query: 240 SEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSG-----K 299
           S +Q QL +L +++  LIDTLR+ E   +++E   +   + Q         CSG     K
Sbjct: 286 SALQSQLVLLKQKQWLLIDTLRQLETEKVDLENTVVDESQRQA-----DNGCSGELRHEK 345

Query: 300 YSECATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVR 359
           +SE   TES DD E+ D  ++  D +E+ F+DT++      L   S K +     T    
Sbjct: 346 FSEGTATESDDDNERGDAAEEEFDEEENTFFDTRD-----FLSSSSFKSSGSGFRTSSF- 405

Query: 360 DSRQDNAEKMHDEQIENFVHS------NVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLA 419
            S +D  E   ++ I+  + S       V+RRK LPDPVEKEK VSLWSMIKDN+GKDL 
Sbjct: 406 SSDEDGFES--EDDIDPSIKSIGCNYPRVKRRKNLPDPVEKEKSVSLWSMIKDNIGKDLT 465

Query: 420 RVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEG 479
           +VCLPVYFNEP+SSLQKC ED+EYS+LLD+A+E+G +GN+L+R+L V+AFAVSGYAS+EG
Sbjct: 466 KVCLPVYFNEPLSSLQKCFEDLEYSYLLDRAFEYGKRGNSLMRILNVAAFAVSGYASTEG 525

Query: 480 RHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWG 539
           R CKPFNPLLGETYEAD+P+KG+RFFSEKVSHHP V+ACHC G GWKFWG+SNLRSKFWG
Sbjct: 526 RICKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMVVACHCDGTGWKFWGDSNLRSKFWG 585

Query: 540 RSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKF 599
           RSIQLDPVGVL L+FDDGE  QWSKVTT+IYNLILGK+YCDH+GTM I G+ EYSC LKF
Sbjct: 586 RSIQLDPVGVLTLQFDDGEILQWSKVTTSIYNLILGKLYCDHYGTMRIEGSAEYSCKLKF 645

Query: 600 KEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNGNPNRSSSSDGS-MLW 659
           KE SI+DRNPHQVHG V++ +G+ VAT+FGKWDE +++V G+ +G    S    G+ +LW
Sbjct: 646 KEQSIIDRNPHQVHGIVQNKSGKTVATMFGKWDESIHFVTGDCSGKGKLSEDMSGAQLLW 705

Query: 660 KSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQR 719
           K  KPP N T+YNLT FAITLNELT  LKERLPPTDSRLRPDQR+LENGE+E AN EK R
Sbjct: 706 KRSKPPGNATKYNLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKLR 765

Query: 720 LERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNEN 750
           LE+RQR +RK+Q+ GWKPRWF +  E   E Y+Y GGYWEAR++  W  C D+FG ++ +
Sbjct: 766 LEQRQRQARKMQERGWKPRWFMK--EKGSESYRYKGGYWEAREDGSWVDCPDIFGHIDSD 809

BLAST of Carg25162 vs. TAIR 10
Match: AT4G08180.2 (OSBP(oxysterol binding protein)-related protein 1C )

HSP 1 Score: 793.1 bits (2047), Expect = 1.9e-229
Identity = 406/720 (56.39%), Postives = 518/720 (71.94%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSY------SKISLLSQPNDDVQLIGKISTNRLS 119
           +AG+LYKW NYG+GWR RWFVL+DGVLSY       KI +  +     ++IG  S   +S
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYKIHGPDKIFVSPETEKGSKVIGDESARMIS 165

Query: 120 RRK-----------QQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVI 179
           R             ++K  G VHLKVSS RES+SDD+RF IFT TK LHLR E+++DR  
Sbjct: 166 RHNRRGGSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTT 225

Query: 180 WIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEF 239
           W++AL + + +FP R+ N  L    N+L++ST+K+  RL+EEG+S+  ++DCEQI+ SEF
Sbjct: 226 WVEALQAVKDMFP-RMSNSELMAPTNNLAMSTEKIRLRLIEEGVSELAIQDCEQIMKSEF 285

Query: 240 SEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSG-----K 299
           S +Q QL +L +++  LIDTLR+ E   +++E   +   + Q         CSG     K
Sbjct: 286 SALQSQLVLLKQKQWLLIDTLRQLETEKVDLENTVVDESQRQA-----DNGCSGELRHEK 345

Query: 300 YSECATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVR 359
           +S  A TES DD E+ D  ++  D +E+ F+DT++      L   S K +     T    
Sbjct: 346 FSGTA-TESDDDNERGDAAEEEFDEEENTFFDTRD-----FLSSSSFKSSGSGFRTSSF- 405

Query: 360 DSRQDNAEKMHDEQIENFVHS------NVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLA 419
            S +D  E   ++ I+  + S       V+RRK LPDPVEKEK VSLWSMIKDN+GKDL 
Sbjct: 406 SSDEDGFES--EDDIDPSIKSIGCNYPRVKRRKNLPDPVEKEKSVSLWSMIKDNIGKDLT 465

Query: 420 RVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEG 479
           +VCLPVYFNEP+SSLQKC ED+EYS+LLD+A+E+G +GN+L+R+L V+AFAVSGYAS+EG
Sbjct: 466 KVCLPVYFNEPLSSLQKCFEDLEYSYLLDRAFEYGKRGNSLMRILNVAAFAVSGYASTEG 525

Query: 480 RHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWG 539
           R CKPFNPLLGETYEAD+P+KG+RFFSEKVSHHP V+ACHC G GWKFWG+SNLRSKFWG
Sbjct: 526 RICKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMVVACHCDGTGWKFWGDSNLRSKFWG 585

Query: 540 RSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKF 599
           RSIQLDPVGVL L+FDDGE  QWSKVTT+IYNLILGK+YCDH+GTM I G+ EYSC LKF
Sbjct: 586 RSIQLDPVGVLTLQFDDGEILQWSKVTTSIYNLILGKLYCDHYGTMRIEGSAEYSCKLKF 645

Query: 600 KEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNGNPNRSSSSDGS-MLW 659
           KE SI+DRNPHQVHG V++ +G+ VAT+FGKWDE +++V G+ +G    S    G+ +LW
Sbjct: 646 KEQSIIDRNPHQVHGIVQNKSGKTVATMFGKWDESIHFVTGDCSGKGKLSEDMSGAQLLW 705

Query: 660 KSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQR 719
           K  KPP N T+YNLT FAITLNELT  LKERLPPTDSRLRPDQR+LENGE+E AN EK R
Sbjct: 706 KRSKPPGNATKYNLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKLR 765

Query: 720 LERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNEN 750
           LE+RQR +RK+Q+ GWKPRWF +  E   E Y+Y GGYWEAR++  W  C D+FG ++ +
Sbjct: 766 LEQRQRQARKMQERGWKPRWFMK--EKGSESYRYKGGYWEAREDGSWVDCPDIFGHIDSD 808

BLAST of Carg25162 vs. TAIR 10
Match: AT4G08180.3 (OSBP(oxysterol binding protein)-related protein 1C )

HSP 1 Score: 787.3 bits (2032), Expect = 1.1e-227
Identity = 404/720 (56.11%), Postives = 516/720 (71.67%), Query Frame = 0

Query: 60  VAGVLYKWTNYGKGWRSRWFVLRDGVLSY------SKISLLSQPNDDVQLIGKISTNRLS 119
           +AG+LYKW NYG+GWR RWFVL+DGVLSY       KI +  +     ++IG  S   +S
Sbjct: 106 IAGILYKWVNYGRGWRPRWFVLQDGVLSYYKIHGPDKIFVSPETEKGSKVIGDESARMIS 165

Query: 120 RRK-----------QQKSGGIVHLKVSSFRESKSDDRRFYIFTATKTLHLRTESKKDRVI 179
           R             ++K  G VHLKVSS RES+SDD+RF IFT TK LHLR E+++DR  
Sbjct: 166 RHNRRGGSSSSCQLRRKPFGEVHLKVSSVRESRSDDKRFSIFTGTKRLHLRAETREDRTT 225

Query: 180 WIQALASSRSLFPLRILNDNLPFVPNDLSVSTQKLEKRLLEEGISDAVVKDCEQIILSEF 239
           W++AL + + +FP R+ N  L    N+L++ST+K+  RL+EEG+S+  ++DCEQI+ SEF
Sbjct: 226 WVEALQAVKDMFP-RMSNSELMAPTNNLAMSTEKIRLRLIEEGVSELAIQDCEQIMKSEF 285

Query: 240 SEMQGQLKVLLEQRSCLIDTLREFEAANIEIEANGIHNGEYQLMKHDFSTECSG-----K 299
           S +Q QL +L +++  LIDTLR+ E   +++E   +   + Q         CSG     K
Sbjct: 286 SALQSQLVLLKQKQWLLIDTLRQLETEKVDLENTVVDESQRQA-----DNGCSGELRHEK 345

Query: 300 YSECATTESSDDVEKQD-LDDGSDVDESFFYDTKESFTEVTLGCGSVKGASDSSDTLKVR 359
           +S  A TES DD E+ D  ++  D +E+ F+DT++      L   S K +     T    
Sbjct: 346 FSGTA-TESDDDNERGDAAEEEFDEEENTFFDTRD-----FLSSSSFKSSGSGFRTSSF- 405

Query: 360 DSRQDNAEKMHDEQIENFVHS------NVQRRKKLPDPVEKEKRVSLWSMIKDNVGKDLA 419
            S +D  E   ++ I+  + S       V+RRK LPDPVEKEK VSLWSMIKDN+GKDL 
Sbjct: 406 SSDEDGFES--EDDIDPSIKSIGCNYPRVKRRKNLPDPVEKEKSVSLWSMIKDNIGKDLT 465

Query: 420 RVCLPVYFNEPISSLQKCCEDMEYSHLLDQAYEHGIQGNNLLRVLKVSAFAVSGYASSEG 479
           +VCLPVYFNEP+SSLQKC ED+EYS+LLD+A+E+G +GN+L+R+L V+AFAVSGYAS+EG
Sbjct: 466 KVCLPVYFNEPLSSLQKCFEDLEYSYLLDRAFEYGKRGNSLMRILNVAAFAVSGYASTEG 525

Query: 480 RHCKPFNPLLGETYEADFPEKGIRFFSEKVSHHPTVIACHCVGRGWKFWGESNLRSKFWG 539
           R CKPFNPLLGETYEAD+P+KG+RFFSEKVSHHP V+ACHC G GWKFWG+SNLRSKFWG
Sbjct: 526 RICKPFNPLLGETYEADYPDKGLRFFSEKVSHHPMVVACHCDGTGWKFWGDSNLRSKFWG 585

Query: 540 RSIQLDPVGVLNLEFDDGERFQWSKVTTNIYNLILGKVYCDHHGTMHIRGNREYSCTLKF 599
           RSIQLDPVGVL L+FDDGE  QWSKVTT+IYNLILGK+YCDH+GTM I G+ EYSC LKF
Sbjct: 586 RSIQLDPVGVLTLQFDDGEILQWSKVTTSIYNLILGKLYCDHYGTMRIEGSAEYSCKLKF 645

Query: 600 KEPSILDRNPHQVHGFVEDGNGRKVATLFGKWDEGMYYVKGESNGNPNRSSSSDGS-MLW 659
           KE SI+DRNPHQVHG V++ +G+ VAT+FGKWDE +++V G+ +G    S    G+ +LW
Sbjct: 646 KEQSIIDRNPHQVHGIVQNKSGKTVATMFGKWDESIHFVTGDCSGKGKLSEDMSGAQLLW 705

Query: 660 KSEKPPPNLTRYNLTSFAITLNELTADLKERLPPTDSRLRPDQRHLENGEYEKANAEKQR 719
           K  KPP N T+YNLT FAITLNELT  LKERLPPTDSRLRPDQR+LENGE+E AN EK R
Sbjct: 706 KRSKPPGNATKYNLTRFAITLNELTPGLKERLPPTDSRLRPDQRYLENGEFEMANTEKLR 765

Query: 720 LERRQRISRKLQDNGWKPRWFHREGEDADEPYKYMGGYWEARKERKWDGCLDVFGELNEN 750
           LE+RQR   ++Q+ GWKPRWF +  E   E Y+Y GGYWEAR++  W  C D+FG ++ +
Sbjct: 766 LEQRQRQVIQMQERGWKPRWFMK--EKGSESYRYKGGYWEAREDGSWVDCPDIFGHIDSD 808