Carg25159 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25159
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
Location	Carg_Chr02: 2390683 .. 2394299 (-)
RNA-Seq Expression	Carg25159
Synteny	Carg25159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTTTAAAATTTAAAATTCGTGCGAAAATGAAAAAACTAATTCTAATCCGTAAGCCCTAAATACATGTCTTTTGCTGAGTAAAACTGAACAGAGCCGGCGGCGGCGATGGGCTGCGCAACCATCGTCATCGTTAGCTTATGAGATCCCTTTCCACTTCTAACCTCCATCTCAAAGCCATAATTTCTCTCCATAAGTCTCTCATGGCTTCCTCTGTCACCCAAATCCTTAAACCAGATTTTCCTCAAGACCTCGATGACCTTCTCTGCTCCGATCCACAGCAACATCAACAAGAGGAAAGTAAATCCGATGACCCATCACTTCTCTTGGTGTCGCGGAGTGGATATTTGAATGGCGGGTCGCGAATTGAGCGAGCCTGGGCTCATTGGTCGAAGCTTGGCCGGCCGAAGTTGATTGTAGCTCCTATGGTGGATAATTCGGAGCTACCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTGCATTCACGGATATTTACCGAGAACGACAAGTACAGGAACGTGGAATTTACCACTTGTCAGGTATATGAAAAGTTCAATCCAAGTTTATGTTATCCTTCTTTCTGTATTTCTGTTCATTCTTCCTTGTTTTTGAAATTTTGAAATGGTTTATAGGAGGACCGCCCATTGTTTGTTCAATTTTGTGCCAATGATCCTGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTAATCTCTATTAATCATCTCTCTAATTCGTATTCTATTGGGCAACTCATACGAATTTTTGCATTCCTTATGATCTTATATGTCAAATGCTTACCTGCTATGCTTCTCGTGGATTGGTGGAGTCAGAATTTGTTTACCAAAACATTTTTTTGTTGATTAATACTGGGGAAACTGTTCCCAAGTTCTAATTATTAGCTTCAATTTGTTACATCAATTGAAGGTGACTATTGGTATTGTGTTCGCAAACCATTATATGTTTGTCAAATCCTAATTTGTTTCACTGAGAGCCAACGATGGGAAAATGCTATCTTGCTTGCTTAGCATAAGGGCTTGCAAACTCTGAGTATTTCGGTTTAACTTGAAACTAGGAATATACATGAACTAACGTCATGACTGAAAAGTTGATACCTGATTATAGGTTGATTGACGGGTTCGATACTACGTGGGATAGCCTGAAATCCATTTGGTTGTTAATCGGTTTGATGTTAAGATGTGTCAATGGTTCAGTGATGTTATTAGTTTTCCTTGGGTGCAGTGAGGAAACCATATTGAGTCTAGACACATATACCCTCCCTGTTTTCCAGCAAGGAGCATATCTTGTGGTAACTGGTTAGAAATCTCTGTCCTTTTGGTGTGTGTCATGTACTCTTGATTGAAGATCCTAGCCAAGTGTAGTTTTGGAGAGCAATTGAAGGAAAATTTGAAAATGAAAAAGCTTGTGCCTGTGTTAAGGTGAATAATTGACTTTGTCTAGCCCCAATTTGAGGAAGCTTGCCAACTTATTTAATGTGGCTTTCCCATTACTAAGGAAAATTACCCATTGAATTTCTTGATTTGGAATGTTAGAGAATGCTGGTTGCTGTATAAAAGAGTAGCTTGGACTCATTGGTTTAAGGAATTTCCAGGAGACAATTAATTATGCTGGTTGCTCTCTTTTAGCTGTTCATGGGAATTTCTATAGAAGAGTAGCCTGGGCTCATTGGTTTAAGGAATTTCCTCTTCTGCAAGATTATTTTGAATGTTAAACTTCGGGGATCAAATTTTGAACAGGCGTAATCCTAGCTAGAGTCATCGTTAGTCGATCAAAAACTTCTTCGAAGTAAGAGGTGTCCTTGCAAAATGTTATCCTATTAGGGCTTGGGAGCTTTGGATTTGATTTTGTTTGTTCATACTCTGTCTCCTCGCGTAGAATTGATACTTTATACATATTTGAAAGGTTCAATTAGTTCGTTATCAGACCCCAGGCCATGGGAGCTTTGGAAAGTCGGATTAGAAAAAAACTTCAATAAGGAATTATGGAGACTTGATTATATTTCGATTTCCTCTCTATTGTCTCCCTTAATTTGAGAGGATCTCCTCCTTGTATAACTTTCCATTGTTCTCAAGTCACTACCAAAGAGGAAGGTAGCATAAACCCATGTTGATTATATTTTAAATTTATAATATTAACGGGTAGAAATTTCAATATCAGTAGATATCACAAGACAAATTGAAAGTACCTCAAGAACTCAAAAAGGTTTCAAAATTGACAAATAAAACTTCGGTATTTATACGTCACGGTACTCGTCGTTGTGACTAACTATATTTACCACTATCATGCTTTACAATTCTGTTAGACTTAAGCAAGTACATTGAATTCAAGTAGGATTTGAGTATCTAATTGATATTAAGAGATCAAGACACTGTGGACCCTTATTAAAGTAGCTTTTAATATAACTTCATCTCACTTGGGAAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACTTCTCTCTAGCAGATAGCGTTCTAAAAACCTTGAGGAGAAGCCCGAAAGGGAAAGCCCAAAAAGGACAATATCTGCTAGCGGTGAGCCTGGTCCGTTACATAATTAATCTAAGAATTAAAATTACAAAAAATTTCTTAAAGAACTTCATGTCGCTTATTTTATTCCTTTTGTGCTATTTTCCAGATTGTTCTCCCTCTCAGTTATGTGTCTGCAACTAACAGAAACAAGATTAAGGATAATCTAATGTCACTTGTTTTTCCAAATGTATTGCGTACCAAATATGTTACATTGTGTCCATATGGTTTACTTACAGTTTCTGAACATGAATTGAGCGCTGTACTAATTCATGCAGGTGTCCTCAGCGTATCGCCAAGCGAGGAAATTATGGGGCGTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGTTGGCTTCCAATCTTGAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAACTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCCATTACTGCTGTTAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAAGATGTTTACGACTGTTTGAAAGAGACCGGGGTCGAGGGCGTTCTTTCAGCCGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACAGCAGAATGGGTAGAGGGCTGTGAAGAAAGCACCAGGGATGGAAACCTGGATCAAGCCGATCTGCTGGTGGATTACTTGAAGCTTTGTGAAAAATACCCCGTCCCGTGGCGAATAATTCGTTCGCACGTGCACAAGTTGTTGGGAGATTGGTTCAAGCTTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTATAATATGGTGGAGCGTTTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTTCTCCTGCAACTGCAATTTTGGCCAATGGTTGA

mRNA sequence

TATTTTAAAATTTAAAATTCGTGCGAAAATGAAAAAACTAATTCTAATCCGTAAGCCCTAAATACATGTCTTTTGCTGAGTAAAACTGAACAGAGCCGGCGGCGGCGATGGGCTGCGCAACCATCGTCATCGTTAGCTTATGAGATCCCTTTCCACTTCTAACCTCCATCTCAAAGCCATAATTTCTCTCCATAAGTCTCTCATGGCTTCCTCTGTCACCCAAATCCTTAAACCAGATTTTCCTCAAGACCTCGATGACCTTCTCTGCTCCGATCCACAGCAACATCAACAAGAGGAAAGTAAATCCGATGACCCATCACTTCTCTTGGTGTCGCGGAGTGGATATTTGAATGGCGGGTCGCGAATTGAGCGAGCCTGGGCTCATTGGTCGAAGCTTGGCCGGCCGAAGTTGATTGTAGCTCCTATGGTGGATAATTCGGAGCTACCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTGCATTCACGGATATTTACCGAGAACGACAAGTACAGGAACGTGGAATTTACCACTTGTCAGGAGGACCGCCCATTGTTTGTTCAATTTTGTGCCAATGATCCTGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATCGCCAAGCGAGGAAATTATGGGGCGTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGTTGGCTTCCAATCTTGAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAACTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCCATTACTGCTGTTAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAAGATGTTTACGACTGTTTGAAAGAGACCGGGGTCGAGGGCGTTCTTTCAGCCGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACAGCAGAATGGGTAGAGGGCTGTGAAGAAAGCACCAGGGATGGAAACCTGGATCAAGCCGATCTGCTGGTGGATTACTTGAAGCTTTGTGAAAAATACCCCGTCCCGTGGCGAATAATTCGTTCGCACGTGCACAAGTTGTTGGGAGATTGGTTCAAGCTTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTATAATATGGTGGAGCGTTTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTTCTCCTGCAACTGCAATTTTGGCCAATGGTTGA

Coding sequence (CDS)

ATGAGATCCCTTTCCACTTCTAACCTCCATCTCAAAGCCATAATTTCTCTCCATAAGTCTCTCATGGCTTCCTCTGTCACCCAAATCCTTAAACCAGATTTTCCTCAAGACCTCGATGACCTTCTCTGCTCCGATCCACAGCAACATCAACAAGAGGAAAGTAAATCCGATGACCCATCACTTCTCTTGGTGTCGCGGAGTGGATATTTGAATGGCGGGTCGCGAATTGAGCGAGCCTGGGCTCATTGGTCGAAGCTTGGCCGGCCGAAGTTGATTGTAGCTCCTATGGTGGATAATTCGGAGCTACCGTTCCGTTTGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTGCATTCACGGATATTTACCGAGAACGACAAGTACAGGAACGTGGAATTTACCACTTGTCAGGAGGACCGCCCATTGTTTGTTCAATTTTGTGCCAATGATCCTGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTTGGGTGTCCTCAGCGTATCGCCAAGCGAGGAAATTATGGGGCGTTTTTAATGGATAACTTACCTCTTGTAAAAGCAATAGTAGAGAAGTTGGCTTCCAATCTTGAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAACTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTAGCTGTTCATGGCCGCACAAGAGATGAAAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCCATTACTGCTGTTAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAAGATGTTTACGACTGTTTGAAAGAGACCGGGGTCGAGGGCGTTCTTTCAGCCGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACAGCAGAATGGGTAGAGGGCTGTGAAGAAAGCACCAGGGATGGAAACCTGGATCAAGCCGATCTGCTGGTGGATTACTTGAAGCTTTGTGAAAAATACCCCGTCCCGTGGCGAATAATTCGTTCGCACGTGCACAAGTTGTTGGGAGATTGGTTCAAGCTTCATCCACACATTAGAGAAGACTTCAATGCACAATCCAAGCTCACCTTTGAATTTCTTTATAATATGGTGGAGCGTTTGAGAGAGTTGCAGGTAAAAATTCCTCTTTATATCAAGGATTCTTCTCCTGCAACTGCAATTTTGGCCAATGGTTGA

Protein sequence

MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG

Homology

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BLAST of Carg25159 vs. NCBI nr
Match: KAG7035164.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 862.8 bits (2228), Expect = 1.2e-246
Identity = 420/420 (100.00%), Postives = 420/420 (100.00%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60
           MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS
Sbjct: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60

Query: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120
           LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM
Sbjct: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120

Query: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180
           LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ
Sbjct: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180

Query: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240
           RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS
Sbjct: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240

Query: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300
           LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL
Sbjct: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300

Query: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360
           SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV
Sbjct: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360

Query: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420
           HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG
Sbjct: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420

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BLAST of Carg25159 vs. NCBI nr
Match: KAG6605182.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 852.0 bits (2200), Expect = 2.1e-243
Identity = 417/420 (99.29%), Postives = 417/420 (99.29%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60
           MRS STSNLHLKAIISLHKSLMASSVTQ LKPDFPQDLDDLLCSDP QHQQEESKSDDPS
Sbjct: 1   MRSPSTSNLHLKAIISLHKSLMASSVTQTLKPDFPQDLDDLLCSDP-QHQQEESKSDDPS 60

Query: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120
           LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM
Sbjct: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120

Query: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180
           LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ
Sbjct: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180

Query: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240
           RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS
Sbjct: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240

Query: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300
           LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL
Sbjct: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300

Query: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360
           SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV
Sbjct: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360

Query: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420
           HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG
Sbjct: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 419

[Close]

BLAST of Carg25159 vs. NCBI nr
Match: XP_022947361.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 851.3 bits (2198), Expect = 3.6e-243
Identity = 414/420 (98.57%), Postives = 418/420 (99.52%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60
           MRSLSTSNLHLKAIISL+KSLMASSVTQ LKPDFPQDLDDLLCSDPQQHQQEESKSDDPS
Sbjct: 1   MRSLSTSNLHLKAIISLYKSLMASSVTQTLKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60

Query: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120
           LL+VSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM
Sbjct: 61  LLVVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120

Query: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180
           LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ
Sbjct: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180

Query: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240
           RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLE+AGCS
Sbjct: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEEAGCS 240

Query: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300
           LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKET VEGVL
Sbjct: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETRVEGVL 300

Query: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360
           SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV
Sbjct: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360

Query: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420
           HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPA+AILANG
Sbjct: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPASAILANG 420

[Close]

BLAST of Carg25159 vs. NCBI nr
Match: XP_023534658.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 850.5 bits (2196), Expect = 6.1e-243
Identity = 414/420 (98.57%), Postives = 417/420 (99.29%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60
           MRS STSNLHLKAIISLHKSLMASSVTQ LKPDFPQDLDDLLCSDPQQHQQEESKSDDPS
Sbjct: 1   MRSPSTSNLHLKAIISLHKSLMASSVTQTLKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60

Query: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120
           LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM
Sbjct: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120

Query: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180
           LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ
Sbjct: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180

Query: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240
           RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS
Sbjct: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240

Query: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300
           LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCL+ETGVEGVL
Sbjct: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLQETGVEGVL 300

Query: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360
           SAETLLENPALFAGFRTAEW+EG EESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV
Sbjct: 301 SAETLLENPALFAGFRTAEWIEGYEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360

Query: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420
           HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPA+AILANG
Sbjct: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPASAILANG 420

[Close]

BLAST of Carg25159 vs. NCBI nr
Match: XP_023006858.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 812.4 bits (2097), Expect = 1.8e-231
Identity = 390/398 (97.99%), Postives = 395/398 (99.25%), Query Frame = 0


Query: 22  MASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLVSRSGYLNGGSRIERAWA 81
           MASSVTQ LKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLV+RSGYLNGGSRIERAWA
Sbjct: 1   MASSVTQTLKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLVTRSGYLNGGSRIERAWA 60

Query: 82  HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ 141
           HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ
Sbjct: 61  HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ 120

Query: 142 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA 201
           EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA
Sbjct: 121 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA 180

Query: 202 IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW 261
           IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW
Sbjct: 181 IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW 240

Query: 262 SAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAEWV 321
           SAITAVKNAVRIPVLANGNIRHMEDVYDCL+ETGVEGVLSAETLLENPALFAGFRT EW+
Sbjct: 241 SAITAVKNAVRIPVLANGNIRHMEDVYDCLQETGVEGVLSAETLLENPALFAGFRTVEWI 300

Query: 322 EGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ 381
           EGCEESTRDGNLDQADLLV+YLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ
Sbjct: 301 EGCEESTRDGNLDQADLLVEYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ 360

Query: 382 SKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILAN 420
           SKLTFEFLYNMVERLRELQVKIPLYIKDSS A+AILAN
Sbjct: 361 SKLTFEFLYNMVERLRELQVKIPLYIKDSSSASAILAN 398

[Close]

BLAST of Carg25159 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.3e-78
Identity = 143/298 (47.99%), Postives = 194/298 (65.10%), Query Frame = 0


Query: 83  WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYR--NVEFTT 142
           WS+ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRALGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 143 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLV 202
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IAKRG YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGRYGAFLQEEWDLL 129

Query: 203 KAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 262
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 263 NWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAE 322
           +W  I AV+ AV IPV ANGNIR ++DV  C+++TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 323 WVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIRED 378
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELADEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of Carg25159 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.2e-78
Identity = 143/298 (47.99%), Postives = 194/298 (65.10%), Query Frame = 0


Query: 83  WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYR--NVEFTT 142
           WS+ L   + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLRGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEV 69

Query: 143 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLV 202
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IAKRG+YGAFL D   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLL 129

Query: 203 KAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 262
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAA 189

Query: 263 NWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAE 322
           +W  I AV+ AV IPV ANGNI+ ++DV  CL++TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 323 WVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIRED 378
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREE 291

[Close]

BLAST of Carg25159 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 2.2e-78
Identity = 142/298 (47.65%), Postives = 195/298 (65.44%), Query Frame = 0


Query: 83  WSK-LGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYR--NVEFTT 142
           WS+ LG  + +VAPMVD SEL +RLL R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 143 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLV 202
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IAKRG+YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQEEWDLL 129

Query: 203 KAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 262
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 263 NWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAE 322
           +W  I AV+ AV IPV ANGNI+ ++DV  C+++TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 323 WVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIRED 378
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

[Close]

BLAST of Carg25159 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.9e-69
Identity = 137/302 (45.36%), Postives = 194/302 (64.24%), Query Frame = 0


Query: 83  WSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFT--TC 142
           ++K+GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E++ YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 143 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVK 202
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLGCPQ IAK+G YG+FL +N  L++
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIE 131

Query: 203 AIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 262
           +I+ KL + L +PV+ KIRIFP+ Q T++YA+M+  AG S+LAVHGR R++K      A+
Sbjct: 132 SIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIAD 191

Query: 263 WSAITAV-KNAVRIPVL-ANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTA 322
           W  I  + KN     VL ANGNI H +D+  C+K TGV+GVLSAE  L NP +F      
Sbjct: 192 WEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFL----- 251

Query: 323 EWVEGCEESTRDGNLDQADLLVDYLKLCEKYPV-----PWRIIRSHVHKLLGDWFKLHPH 376
                    T    +D  D+  +YL +  ++ +         I+ H+ KL+     +H  
Sbjct: 252 --PPSSPLMTLYPRID--DMCEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTD 304

[Close]

BLAST of Carg25159 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.1e-64
Identity = 131/315 (41.59%), Postives = 188/315 (59.68%), Query Frame = 0


Query: 83  WSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTT--- 142
           + K+GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  + KYR   +++   
Sbjct: 23  FDKIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDG 82

Query: 143 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLV 202
              DRPL VQFCANDP+ LL AA+ VE  CD VDLNLGCPQ IAK+G+YG+FLM+   L+
Sbjct: 83  SSVDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLI 142

Query: 203 KAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 262
             ++  L  NL+VPV+ KIRIF + + ++NYA+M+ DAG   L VHGR R++K  K   A
Sbjct: 143 HNLINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLA 202

Query: 263 NWSAITAVKNAV--RIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRT 322
           NW  I  +++ +       ANGNI + ED+  C++  G + V+SAE  L NP +F   +T
Sbjct: 203 NWETIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT 262

Query: 323 AEWVEGCEESTRDGNLDQADLLV----DYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPH 382
                      ++    + D ++      +K C++       ++SH  K+L  +   H  
Sbjct: 263 ---------KNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTD 322

Query: 383 IREDF-NAQSKLTFE 388
           IR       +K T+E
Sbjct: 323 IRSTLATMNAKATWE 328

[Close]

BLAST of Carg25159 vs. ExPASy TrEMBL
Match: A0A6J1G672 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111451248 PE=4 SV=1)

HSP 1 Score: 851.3 bits (2198), Expect = 1.7e-243
Identity = 414/420 (98.57%), Postives = 418/420 (99.52%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60
           MRSLSTSNLHLKAIISL+KSLMASSVTQ LKPDFPQDLDDLLCSDPQQHQQEESKSDDPS
Sbjct: 1   MRSLSTSNLHLKAIISLYKSLMASSVTQTLKPDFPQDLDDLLCSDPQQHQQEESKSDDPS 60

Query: 61  LLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120
           LL+VSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM
Sbjct: 61  LLVVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPM 120

Query: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180
           LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ
Sbjct: 121 LHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQ 180

Query: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCS 240
           RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLE+AGCS
Sbjct: 181 RIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEEAGCS 240

Query: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVL 300
           LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKET VEGVL
Sbjct: 241 LLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETRVEGVL 300

Query: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360
           SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV
Sbjct: 301 SAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHV 360

Query: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILANG 420
           HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPA+AILANG
Sbjct: 361 HKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPASAILANG 420

[Close]

BLAST of Carg25159 vs. ExPASy TrEMBL
Match: A0A6J1L641 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111499526 PE=4 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 8.9e-232
Identity = 390/398 (97.99%), Postives = 395/398 (99.25%), Query Frame = 0


Query: 22  MASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLVSRSGYLNGGSRIERAWA 81
           MASSVTQ LKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLV+RSGYLNGGSRIERAWA
Sbjct: 1   MASSVTQTLKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLLLVTRSGYLNGGSRIERAWA 60

Query: 82  HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ 141
           HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ
Sbjct: 61  HWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQ 120

Query: 142 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA 201
           EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA
Sbjct: 121 EDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKA 180

Query: 202 IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW 261
           IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW
Sbjct: 181 IVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANW 240

Query: 262 SAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFAGFRTAEWV 321
           SAITAVKNAVRIPVLANGNIRHMEDVYDCL+ETGVEGVLSAETLLENPALFAGFRT EW+
Sbjct: 241 SAITAVKNAVRIPVLANGNIRHMEDVYDCLQETGVEGVLSAETLLENPALFAGFRTVEWI 300

Query: 322 EGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ 381
           EGCEESTRDGNLDQADLLV+YLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ
Sbjct: 301 EGCEESTRDGNLDQADLLVEYLKLCEKYPVPWRIIRSHVHKLLGDWFKLHPHIREDFNAQ 360

Query: 382 SKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAILAN 420
           SKLTFEFLYNMVERLRELQVKIPLYIKDSS A+AILAN
Sbjct: 361 SKLTFEFLYNMVERLRELQVKIPLYIKDSSSASAILAN 398

[Close]

BLAST of Carg25159 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.1e-216
Identity = 373/424 (87.97%), Postives = 396/424 (93.40%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEE----SKS 60
           MRS S S+LHLK+I+SL KS MASS  Q    DFPQDLDDLLCSDP   +Q+E    +KS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQ---TDFPQDLDDLLCSDPHTQRQDECSSLTKS 60

Query: 61  DDPSLLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           +D +LLL S SGYLNG SRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTEN+KYRN+EFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLED 240
           GCPQRIA+RGNYGAFLMDNLPLVKAIVEK+ASNL+VPVSCKIR+FPNLQDTINYARMLED
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDV +CL+ETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRII 360
           EGVLSAETLLENPALFAGFRTA+W  GCEES+RDGNLDQADLLVDYLKLCEKYPVPWR+I
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAI 420
           RSHVHKLLGDWFK+HPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKD SPA+AI
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKD-SPASAI 420

[Close]

BLAST of Carg25159 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 760.8 bits (1963), Expect = 3.1e-216
Identity = 372/424 (87.74%), Postives = 398/424 (93.87%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEE----SKS 60
           MRS S S+LHLK+I+SL KS MASS TQ    DFPQDLDDLLCSDP    Q+E    +KS
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQ---TDFPQDLDDLLCSDPHTQPQDECSSLTKS 60

Query: 61  DDPSLLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           +D +LLL S SGYL+G SRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  NDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTEN+KYRN+EFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLED 240
           GCPQRIA+RGNYGAFLMDNLPLVKAIVEK+ASNL+VPVSCKIR+FPNLQDTINYARMLED
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAI+AVKNA+RIPVLANGNIRHMEDV++CL+ETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRII 360
           EGVLSAETLLENPALFAGFRTA+W  GCEES+RDGNLDQADLLVDYLKLCEKYPVPWR+I
Sbjct: 301 EGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRMI 360

Query: 361 RSHVHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAI 420
           RSHVHKLLGDWFK+HPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKD SPA+AI
Sbjct: 361 RSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKD-SPASAI 420

[Close]

BLAST of Carg25159 vs. ExPASy TrEMBL
Match: A0A6J1D4R6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3673 GN=LOC111017278 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.0e-216
Identity = 365/424 (86.08%), Postives = 400/424 (94.34%), Query Frame = 0


Query: 1   MRSLSTSNLHLKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEE----SKS 60
           MRSL+ S+LHLK++ SLH SLMASS +Q +KPD P+ LDDLLCS+ QQ Q+EE    +KS
Sbjct: 1   MRSLAISHLHLKSVFSLHSSLMASSFSQTIKPDLPEHLDDLLCSNQQQQQREECSSVTKS 60

Query: 61  DDPSLLLVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120
           D+PSLLL S +GYL+G SRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA
Sbjct: 61  DEPSLLLGSPNGYLSGDSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAA 120

Query: 121 YTPMLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180
           YTPMLHSRIFTEN+KYRN+EFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL
Sbjct: 121 YTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNL 180

Query: 181 GCPQRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLED 240
           GCPQRIA+RGNYGAFLMDNLPLVKAIV+KL+SNL+VPVSCKIRIFPNLQDT+NYARMLED
Sbjct: 181 GCPQRIARRGNYGAFLMDNLPLVKAIVQKLSSNLQVPVSCKIRIFPNLQDTLNYARMLED 240

Query: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGV 300
           AGCSLLAVHGRTRDEKDGKKFRANWSAI+AVKNAV+IPVLANGNIRHMEDV +CL+ETGV
Sbjct: 241 AGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVQIPVLANGNIRHMEDVNNCLQETGV 300

Query: 301 EGVLSAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRII 360
           EGVLSAETLLENPALFAGFRTAEWVEGC+E+ RDGNLDQA LLV+YLKLCE+YPVPWRII
Sbjct: 301 EGVLSAETLLENPALFAGFRTAEWVEGCDENCRDGNLDQAALLVEYLKLCEQYPVPWRII 360

Query: 361 RSHVHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPATAI 420
           RSHVHK+LGDWFK+HPH+REDFNAQ KLTFEFLY MV+RLRELQVKIPLY+KD SPA+ I
Sbjct: 361 RSHVHKMLGDWFKIHPHVREDFNAQFKLTFEFLYIMVDRLRELQVKIPLYLKD-SPASEI 420

[Close]

BLAST of Carg25159 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 604.7 bits (1558), Expect = 5.5e-173
Identity = 296/407 (72.73%), Postives = 341/407 (83.78%), Query Frame = 0


Query: 4   LSTSNLH-LKAIISLHKSLMASSVTQILKPDFPQDLDDLLCSDPQQHQQEESKSDDPSLL 63
           L+ SNL  L+   SL     A +     KPD  Q LDD+LCS+ +  Q EE+    P+  
Sbjct: 3   LNLSNLRFLRTRKSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEETVDTAPA-S 62

Query: 64  LVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLH 123
           L S S  L+  +R+ERAWAHW KLGRPK IVAPMVDNSELPFRLLC+KYGA+AAYTPMLH
Sbjct: 63  LGSPSRVLSIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLH 122

Query: 124 SRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRI 183
           SRIFTE +KYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCPQRI
Sbjct: 123 SRIFTETEKYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRI 182

Query: 184 AKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLL 243
           A+RGNYGAFLMDNLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLEDAGCSLL
Sbjct: 183 ARRGNYGAFLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLL 242

Query: 244 AVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSA 303
           AVHGRTRDEKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV +C+KETGVEGVLSA
Sbjct: 243 AVHGRTRDEKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSA 302

Query: 304 ETLLENPALFAGFRTAEWV-EGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRIIRSHVH 363
           ETLLENPA FAGFRTAEW  +  EE   DG LDQ DL+V+YLKLCEK+PVPWR+IRSHVH
Sbjct: 303 ETLLENPAAFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVH 362

Query: 364 KLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIK 409
           K+LG+WF++HP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 363 KMLGEWFRIHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

[Close]

BLAST of Carg25159 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 129.8 bits (325), Expect = 5.1e-30
Identity = 79/247 (31.98%), Postives = 121/247 (48.99%), Query Frame = 0


Query: 90  KLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVE------------- 149
           KL++APMV    L FR+L  +YGA+  Y   +      + ++  NV              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 150 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFLM 209
             F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L+
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALL 125

Query: 210 DNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 269
               L+  I+  L  NL+VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   D
Sbjct: 126 SKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIAD 185

Query: 270 GKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALFA 321
             +  A W  I  V  A+ IPV+ANG++   +D       TG   V+ A   + N ++F+
Sbjct: 186 RPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFS 245

[Close]

BLAST of Carg25159 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 113.2 bits (282), Expect = 5.0e-25
Identity = 65/188 (34.57%), Postives = 98/188 (52.13%), Query Frame = 0


Query: 134 NVEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIAKRGNYGAFL 193
           NV F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 194 MDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 253
           +    L+  I+  L  NL+VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 254 DGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETGVEGVLSAETLLENPALF 313
           D  +  A W  I  V  A+ IPV+ANG++   +D       TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 314 AGFRTAEW 321
           +    + W
Sbjct: 206 SPKGKSHW 211

[Close]

BLAST of Carg25159 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 3.2e-16
Identity = 53/200 (26.50%), Postives = 92/200 (46.00%), Query Frame = 0


Query: 88  RPKLIVAPMVDNSELPFRLLCRKYGAEAAYTPMLHSRIFTENDKYRNVEFTTCQEDRPLF 147
           R KL +AP+     LPFR LC+  GA+     M       +              +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 148 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIAKRGNYGAFLMDNLPLVKAIVEK 207
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 208 LASNLEVPVSCKIR--IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSA 267
            +S +E P++ K+R   F       +    + + G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 268 I-TAVKNA-VRIPVLANGNI 282
           I    KNA   + V+ NG++
Sbjct: 517 IYQCTKNATTNLQVIGNGDV 534

[Close]

BLAST of Carg25159 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.7e-09
Identity = 40/135 (29.63%), Postives = 65/135 (48.15%), Query Frame = 0


Query: 89  PKLIVAPMVDNSELPFRLLCRKYGAEA-AYTPMLHSRIFTENDKYRNVEFTTCQEDRPLF 148
           P   VAPM+D ++  +R L R     A  YT M+ +          +       +  P+ 
Sbjct: 52  PSFSVAPMMDWTDNHYRTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIV 111

Query: 149 VQFCANDPDVLLEAARRVEPY-CDYVDLNLGCPQ-RIAKRGNYGAFLMDNLPLVKAIVEK 208
           +Q   ++ + L +AA+  + Y  D ++LN GCP  ++A  G +G  LM    LV   +  
Sbjct: 112 LQLGGSNVENLAKAAKLSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSA 171

Query: 209 LASNLEVPVSCKIRI 221
           +A+N  VPV+ K RI
Sbjct: 172 IAANTNVPVTVKCRI 186

The following BLAST results are available for this feature:

BLAST of Carg25159 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7035164.1	1.2e-246	100.00	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosp...	[more]
KAG6605182.1	2.1e-243	99.29	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein, partial [Cucurbita...	[more]
XP_022947361.1	3.6e-243	98.57	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata]	[more]
XP_023534658.1	6.1e-243	98.57	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo...	[more]
XP_023006858.1	1.8e-231	97.99	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima]	[more]

BLAST of Carg25159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8K582	1.3e-78	47.99	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101...	[more]
Q6P1R4	2.2e-78	47.99	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=...	[more]
Q8C2P3	2.2e-78	47.65	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN...	[more]
Q9HGN6	1.9e-69	45.36	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st...	[more]
P53759	1.1e-64	41.59	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str...	[more]

BLAST of Carg25159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G672	1.7e-243	98.57	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1L641	8.9e-232	97.99	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1JGU3	3.1e-216	87.97	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1FWR0	3.1e-216	87.74	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1D4R6	9.0e-216	86.08	tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3...	[more]

BLAST of Carg25159 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G67220.1	5.5e-173	72.73	FMN-linked oxidoreductases superfamily protein	[more]
AT3G49640.1	5.1e-30	31.98	Aldolase-type TIM barrel family protein	[more]
AT3G49640.2	5.0e-25	34.57	Aldolase-type TIM barrel family protein	[more]
AT4G38890.1	3.2e-16	26.50	FMN-linked oxidoreductases superfamily protein	[more]
AT3G63510.1	1.7e-09	29.63	FMN-linked oxidoreductases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 88..321 e-value: 2.6E-79 score: 267.8
IPR035587	DUS-like, FMN-binding domain	PFAM	PF01207	Dus	coord: 92..384 e-value: 1.9E-73 score: 247.3
IPR035587	DUS-like, FMN-binding domain	CDD	cd02801	DUS_like_FMN	coord: 90..320 e-value: 2.65857E-106 score: 311.736
None	No IPR available	PANTHER	PTHR11082	TRNA-DIHYDROURIDINE SYNTHASE	coord: 72..408
None	No IPR available	PANTHER	PTHR11082:SF35	BNAA09G07510D PROTEIN	coord: 72..408
None	No IPR available	SUPERFAMILY	51395	FMN-linked oxidoreductases	coord: 89..365
IPR018517	tRNA-dihydrouridine synthase, conserved site	PROSITE	PS01136	UPF0034	coord: 172..190

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25159-RA	Carg25159-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0002943	tRNA dihydrouridine synthesis
biological_process	GO:0008033	tRNA processing
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0017150	tRNA dihydrouridine synthase activity
molecular_function	GO:0003824	catalytic activity