Carg25148 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg25148 vs. NCBI nr
Match: KAG7035175.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1515.7 bits (3923), Expect = 0.0e+00
Identity = 813/813 (100.00%), Postives = 813/813 (100.00%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESH 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESH
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESH 420

Query: 421 SHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNH 480
           SHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNH
Sbjct: 421 SHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNH 480

Query: 481 IEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSS 540
           IEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSS
Sbjct: 481 IEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSS 540

Query: 541 SLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSIN 600
           SLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSIN
Sbjct: 541 SLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSIN 600

Query: 601 DNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVEL 660
           DNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVEL
Sbjct: 601 DNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVEL 660

Query: 661 LGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANLA 720
           LGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANLA
Sbjct: 661 LGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANLA 720

Query: 721 TIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPL 780
           TIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPL
Sbjct: 721 TIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPL 780

Query: 781 VALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           VALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 VALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 813

BLAST of Carg25148 vs. NCBI nr
Match: XP_023533630.1 (U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo] >XP_023533632.1 U-box domain-containing protein 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1508.4 bits (3904), Expect = 0.0e+00
Identity = 811/814 (99.63%), Postives = 813/814 (99.88%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERL+TIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLITIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE-S 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE S
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDEQS 420

Query: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480
           HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN
Sbjct: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480

Query: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540
           HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS
Sbjct: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIE+LVNLLLSADSKIQENAVTALLNLSI
Sbjct: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEHLVNLLLSADSKIQENAVTALLNLSI 600

Query: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660
           NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE
Sbjct: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660

Query: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720
           LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL
Sbjct: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720

Query: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780
           ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP
Sbjct: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780

Query: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814

BLAST of Carg25148 vs. NCBI nr
Match: XP_022947626.1 (U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022947627.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022947628.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022947629.1 U-box domain-containing protein 4-like [Cucurbita moschata] >XP_022947632.1 U-box domain-containing protein 4-like [Cucurbita moschata])

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 809/814 (99.39%), Postives = 810/814 (99.51%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQ EALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQGEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELS SSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSLSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE-S 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLI KPSMSPSRTNVPNSSEDE S
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLITKPSMSPSRTNVPNSSEDEQS 420

Query: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480
           HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN
Sbjct: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480

Query: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540
           HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS
Sbjct: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI
Sbjct: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600

Query: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660
           NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKV+IGRSGAIGPLVE
Sbjct: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVRIGRSGAIGPLVE 660

Query: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720
           LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL
Sbjct: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720

Query: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780
           ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP
Sbjct: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780

Query: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814

BLAST of Carg25148 vs. NCBI nr
Match: XP_023006968.1 (U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023006969.1 U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023006970.1 U-box domain-containing protein 4-like [Cucurbita maxima] >XP_023006971.1 U-box domain-containing protein 4-like [Cucurbita maxima])

HSP 1 Score: 1484.2 bits (3841), Expect = 0.0e+00
Identity = 801/814 (98.40%), Postives = 806/814 (99.02%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYI+LQPTLKYYHKIEG  KLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYIHLQPTLKYYHKIEGGLKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYL L+SEALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLALRSEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSSSSLEH VQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSSSLEHSVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVT MHERL+TIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTCMHERLITIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE-S 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE S
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDEQS 420

Query: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480
           H HNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN
Sbjct: 421 HIHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480

Query: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540
           HIEPELPPRLADSRSRGNMMWLRPSERF+SKITT SANETRPDISAIEAQVRKVVEELKS
Sbjct: 481 HIEPELPPRLADSRSRGNMMWLRPSERFSSKITTLSANETRPDISAIEAQVRKVVEELKS 540

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIE+LVNLLLSADSKIQENAVTALLNLSI
Sbjct: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEHLVNLLLSADSKIQENAVTALLNLSI 600

Query: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660
           NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE
Sbjct: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660

Query: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720
           LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL
Sbjct: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720

Query: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780
           ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP
Sbjct: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780

Query: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814

BLAST of Carg25148 vs. NCBI nr
Match: KAG6605197.1 (U-box domain-containing protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1338.2 bits (3462), Expect = 0.0e+00
Identity = 726/740 (98.11%), Postives = 727/740 (98.24%), Query Frame = 0

Query: 49  DAVVDSDIASDEELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSL 108
           + VVDSDIASDEELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKI KSSL
Sbjct: 39  NGVVDSDIASDEELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIEKSSL 98

Query: 109 DIFQLLRSSNENLPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVL 168
           DIFQLLRSSNENLPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVL
Sbjct: 99  DIFQLLRSSNENLPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVL 158

Query: 169 VKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQ 228
           VKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQ
Sbjct: 159 VKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQ 218

Query: 229 SQSSGPVSIPTDFCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHT 288
           SQSSGPVSIPTDFCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHT
Sbjct: 219 SQSSGPVSIPTDFCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHT 278

Query: 289 NLIPNYTVKALIANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHR 348
           NLIPNYTVKALIANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHR
Sbjct: 279 NLIPNYTVKALIANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHR 338

Query: 349 DGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSR 408
           DGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSR
Sbjct: 339 DGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSR 398

Query: 409 TNVPNSSEDE-SHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESK 468
           TNVPNSSEDE SHSHNRKASTSSAVSNANLSRGTSGEANES          GSDAAGESK
Sbjct: 399 TNVPNSSEDEQSHSHNRKASTSSAVSNANLSRGTSGEANES----------GSDAAGESK 458

Query: 469 SEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIE 528
           SEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIE
Sbjct: 459 SEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIE 518

Query: 529 AQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQ 588
           AQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQ
Sbjct: 519 AQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQ 578

Query: 589 ENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVK 648
           ENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVK
Sbjct: 579 ENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVK 638

Query: 649 IGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAG 708
           IGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAG
Sbjct: 639 IGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAG 698

Query: 709 MVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRH 768
           MVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRH
Sbjct: 699 MVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRH 758

Query: 769 CSMVLQEGAVPPLVALSQSG 788
           CSMVLQEGAVPPLVALSQSG
Sbjct: 759 CSMVLQEGAVPPLVALSQSG 768

BLAST of Carg25148 vs. ExPASy Swiss-Prot
Match: O22193 (U-box domain-containing protein 4 OS=Arabidopsis thaliana OX=3702 GN=PUB4 PE=1 SV=3)

HSP 1 Score: 863.2 bits (2229), Expect = 2.3e-249
Identity = 509/833 (61.10%), Postives = 606/833 (72.75%), Query Frame = 0

Query: 6   LKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDEELIKA 65
           +++LLR ISSF  LSSS +I+L P  KYY ++E + ++LKPI D VV SD   DE+L KA
Sbjct: 1   MEVLLRSISSFLNLSSSKHIDLDPFEKYYKRVEELLRVLKPIADVVVTSDFVFDEKLGKA 60

Query: 66  FEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNENLPEEL 125
           FEEL   VD+   LF +WQ  SSKVY VLQ E+L+ K+  + +D FQ L SS  +LP+EL
Sbjct: 61  FEELTQDVDQSIDLFRSWQAFSSKVYFVLQIESLLPKMRDTIVDTFQFLMSSKNHLPDEL 120

Query: 126 SSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSNQEILI 185
           S +SLE C++KIKH+  EEISSVI  A+R+Q +G+  PS ++LVK+ ++  LRSNQEILI
Sbjct: 121 SPASLEQCLEKIKHLSYEEISSVIDGALRDQRDGVG-PSPEILVKIGENTGLRSNQEILI 180

Query: 186 EAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTDFCCPL 245
           EAVALE+ KE AEQ+E   E E +DQ++ +V RMHERLL IKQ+Q+S  V+I  DF CPL
Sbjct: 181 EAVALERQKEMAEQSENNAEVEFLDQLIVIVNRMHERLLLIKQTQTSS-VAILADFFCPL 240

Query: 246 SLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWCE 305
           SLE+MTDPVIV+SGQTYE+ FIK WID+GL VCPKTRQTL HT LIPNYTVKALIANWCE
Sbjct: 241 SLEVMTDPVIVSSGQTYEKAFIKRWIDLGLKVCPKTRQTLTHTTLIPNYTVKALIANWCE 300

Query: 306 TNNVKLSDPSRSV----LGSFESDTHREPLF------------SHSPQSARSVLG----- 365
           TN+VKL DP++S     L    S T   P              SH   ++ S  G     
Sbjct: 301 TNDVKLPDPNKSTSLNELSPLLSCTDSIPSTGADVSARKVSNKSHDWDASSSETGKPSFS 360

Query: 366 --GTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKP 425
              T R+G+S     S    S    +G+  G+D  R  L   ED+S    E   D   + 
Sbjct: 361 SRATEREGASPSRPASALGASSPGISGNGYGLDARRGSLNDFEDRSNDSRELRTDAPGRS 420

Query: 426 SM-SPSRTNVPNSSEDESHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSD 485
           S+ S +R +V N    E+H H+R  S +S VSN    R    +ANE+   S + T Y SD
Sbjct: 421 SVSSTTRGSVENGQTSENH-HHRSPSATSTVSNEEFPR---ADANENSEESAHATPYSSD 480

Query: 486 AAGESKSEPLAAASSTPNHIE-PELPPRLADSRSRGNMMWLRPSERFASKITTPSANETR 545
           A+GE +S PLAA +S     +  +  P+  D R+RG   W RPSER  S+I +  +NETR
Sbjct: 481 ASGEIRSGPLAATTSAATRRDLSDFSPKFMDRRTRG-QFWRRPSERLGSRIVSAPSNETR 540

Query: 546 PDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLL 605
            D+S +E QV+K+VEELKSSSL+T R ATAELRLLAKHNMDNRIVI   GAI  LV LL 
Sbjct: 541 RDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLY 600

Query: 606 SADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSV 665
           S DS  QENAVTALLNLSINDNNK AIA A AIEPLIHVL+ GS EAKENSAATLFSLSV
Sbjct: 601 STDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSV 660

Query: 666 IEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVE 725
           IEENK+KIG+SGAIGPLV+LLG GTPRGKKDAATALFNLSI  ENKA IVQ+GAVR+L++
Sbjct: 661 IEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLID 720

Query: 726 LMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQL 785
           LMDPAAGMVDKAVAVLANLATIPEGR+AIGQEGGIP+LVEVVELGSARGKENAAAALLQL
Sbjct: 721 LMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQL 780

Query: 786 CTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
            T S R C+MVLQEGAVPPLVALSQSGT RA+EKAQALLS+FR+QRHGN+GRG
Sbjct: 781 STNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGNAGRG 826

BLAST of Carg25148 vs. ExPASy Swiss-Prot
Match: Q5XEZ8 (U-box domain-containing protein 2 OS=Arabidopsis thaliana OX=3702 GN=PUB2 PE=2 SV=1)

HSP 1 Score: 644.8 bits (1662), Expect = 1.3e-183
Identity = 398/815 (48.83%), Postives = 514/815 (63.07%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           M VS L++LL +ISS+  LSS D ++  P  KYY + E + KL+KP+L+ ++DSD A  E
Sbjct: 5   MEVSWLRVLLDNISSYLSLSSMDDLSSNPAHKYYTRGEDIGKLIKPVLENLIDSDAAPSE 64

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
            L   FEEL   VDELR  F++WQPLS++++ VL+ E+L +K+ +SSL++FQLL+   ++
Sbjct: 65  LLNNGFEELAQYVDELREQFQSWQPLSTRIFYVLRIESLASKLRESSLEVFQLLKHCEQH 124

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LP +L S S E C++ +K + R+EIS  I  A+++Q +G+  P+S+VLVK+A+S  LRSN
Sbjct: 125 LPADLISPSFEECIELVKLVARDEISYTIDQALKDQKKGVG-PTSEVLVKIAESTGLRSN 184

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEIL+E V L  +KE AE  +   EAE +D +++L T+MHE L  IKQ+Q   PV +P+D
Sbjct: 185 QEILVEGVVLTNMKEDAELTDNDTEAEYLDGLISLTTQMHEYLSDIKQAQLRCPVRVPSD 244

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           F C LSLELMTDPVIVASGQT+ERVFI+ WIDMGL VCPKTRQ L HT L PN+ V+A +
Sbjct: 245 FRCSLSLELMTDPVIVASGQTFERVFIQKWIDMGLMVCPKTRQALSHTTLTPNFIVRAFL 304

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           A+WCETNNV   DP   +        H    F    +S R+         SSS   HS S
Sbjct: 305 ASWCETNNVYPPDPLELI--------HSSEPFPLLVESVRA---------SSSENGHSES 364

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESH 420
            D                                                    +E+   
Sbjct: 365 LD----------------------------------------------------AEELRQ 424

Query: 421 SHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNH 480
             +R AS    V                           S+   ++K    AAA      
Sbjct: 425 VFSRSASAPGIV---------------------------SEVVCKTKRNNNAAADR---- 484

Query: 481 IEPELPPRLADSRSRGNMMWLRPSER-FASKITTPSANETRPDISAIEAQVRKVVEELKS 540
                      S +R N  W  P ER +      P+        S+IE +V+K++++LKS
Sbjct: 485 -----------SLTRSNTPWKFPEERHWRHPGIIPATVRETGSSSSIETEVKKLIDDLKS 544

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSL+T R ATA +R+LA+++ DNRIVIA+C AI  LV+LL S D +IQ +AVT LLNLSI
Sbjct: 545 SSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYSTDERIQADAVTCLLNLSI 604

Query: 601 NDNNKNAIAQANAIEPLIHVLKTG-SPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLV 660
           NDNNK+ IA++ AI PLIHVLKTG   EAK NSAATLFSLSVIEE K +IG +GAI PLV
Sbjct: 605 NDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSVIEEYKTEIGEAGAIEPLV 664

Query: 661 ELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLAN 720
           +LLG G+  GKKDAATALFNLSI HENK ++++AGAVR+LVELMDPA GMV+KAV VLAN
Sbjct: 665 DLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVELMDPAFGMVEKAVVVLAN 707

Query: 721 LATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVP 780
           LAT+ EG+ AIG+EGGIPVLVEVVELGSARGKENA AALLQLCT S + C+ V++EG +P
Sbjct: 725 LATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQLCTHSPKFCNNVIREGVIP 707

Query: 781 PLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           PLVAL++SGTAR KEKAQ LL +F++ R  N  RG
Sbjct: 785 PLVALTKSGTARGKEKAQNLLKYFKAHRQSNQRRG 707

BLAST of Carg25148 vs. ExPASy Swiss-Prot
Match: Q8GWV5 (U-box domain-containing protein 3 OS=Arabidopsis thaliana OX=3702 GN=PUB3 PE=2 SV=2)

HSP 1 Score: 499.2 bits (1284), Expect = 8.8e-140
Identity = 338/811 (41.68%), Postives = 477/811 (58.82%), Query Frame = 0

Query: 6   LKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDEELIKA 65
           ++ LL  IS +  L +   I   P       +  + KLLKP+LD VVD  I SD+ L K 
Sbjct: 6   VRCLLNSISRYLHLVACQTIRFNPIQTCIGNMVLLLKLLKPLLDEVVDCKIPSDDCLYKG 65

Query: 66  FEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNENLPEEL 125
            E+LD  V++ R   E+W P  SK++ V Q E L+ K+   SL+I ++L   +++ P   
Sbjct: 66  CEDLDSVVNQAREFLEDWSPKLSKLFGVFQCEVLLGKVQTCSLEISRILLQLSQSSPVTS 125

Query: 126 SSSSLEHCVQKIKHIGRE-EISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSNQEIL 185
           S  S+E CVQ+ +   +E  +  ++++A+RNQ + I    ++ L  +   L L SNQ++L
Sbjct: 126 SVQSVERCVQETESFKQEGTLMELMENALRNQKDDITSLDNNHLESIIQMLGLISNQDLL 185

Query: 186 IEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTDFCCP 245
            E++ +EK +  ++ ++   + E  +Q++ LV  + E +L  +  + +  +SIP  F CP
Sbjct: 186 KESITVEKERIRSQASKSEEDMEQTEQLIELVLCIREHMLKTEFLEVAKGISIPPYFRCP 245

Query: 246 LSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWC 305
           LS ELM DPVIVASGQT++R  IK W+D GL VCP+TRQ L H  LIPNYTVKA+IA+W 
Sbjct: 246 LSTELMLDPVIVASGQTFDRTSIKKWLDNGLAVCPRTRQVLTHQELIPNYTVKAMIASWL 305

Query: 306 ETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFSEDSL 365
           E N + L+  S       ++ +    + S       S         SSSL S S    SL
Sbjct: 306 EANRINLATNSCHQYDGGDASSMANNMGSQDFNRTESFRFSLR---SSSLTSRS----SL 365

Query: 366 SNDAGDER-GIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESHSHN 425
               G E+  I+VS  L   ++ +  ++ E          +SP           +S++H+
Sbjct: 366 ETGNGFEKLKINVSASLCGESQSKDLEIFE---------LLSPG----------QSYTHS 425

Query: 426 RKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNHIEP 485
           R  S  S VS+ +         +E+ ++  N       ++ E   +    +S+  NH   
Sbjct: 426 RSESVCSVVSSVDY---VPSVTHETESILGN-----HQSSSEMSPKKNLESSNNVNH--- 485

Query: 486 ELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSSSLE 545
                                E  A+K    S ++     +   +   K+VE+LKS S +
Sbjct: 486 ---------------------EHSAAKTYECSVHDLDDSGTMTTSHTIKLVEDLKSGSNK 545

Query: 546 TLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSINDNN 605
               A AE+R L  ++++NR+ I +CGAI  L++LL S +   QE+AVTALLNLSI++ N
Sbjct: 546 VKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAVTALLNLSISELN 605

Query: 606 KNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRS-GAIGPLVELLG 665
           K  I +  AIEPL+HVL TG+  AKENSAA+LFSLSV++ N+ +IG+S  AI  LV LLG
Sbjct: 606 KAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQSNAAIQALVNLLG 665

Query: 666 IGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANLATI 725
            GT RGKKDAA+ALFNLSI H+NKARIVQA AV++LVEL+DP   MVDKAVA+LANL+ +
Sbjct: 666 KGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELLDPDLEMVDKAVALLANLSAV 725

Query: 726 PEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPLVA 785
            EGR AI +EGGIP+LVE V+LGS RGKENAA+ LLQLC  S + C++VLQEGA+PPLVA
Sbjct: 726 GEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTLVLQEGAIPPLVA 758

Query: 786 LSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LSQSGT RAKEKAQ LLSHFR+QR     +G
Sbjct: 786 LSQSGTQRAKEKAQQLLSHFRNQRDARMKKG 758

BLAST of Carg25148 vs. ExPASy Swiss-Prot
Match: Q9SNC6 (U-box domain-containing protein 13 OS=Arabidopsis thaliana OX=3702 GN=PUB13 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 8.1e-77
Identity = 249/792 (31.44%), Postives = 356/792 (44.95%), Query Frame = 0

Query: 42  KLLKPILDAVVDSDIASDEELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALIT 101
           KLL P+ + + +S+    E+ +K    L  ++   +  +  +    SK+YLV++ E + +
Sbjct: 41  KLLVPMFEEIRESNEPISEDTLKTLMNLKEAMCSAKD-YLKFCSQGSKIYLVMEREQVTS 100

Query: 102 KIGKSSLDIFQLLRSSNENLP-EELSSS-----SLEHCVQKIKHI-GREEISSVIKDAIR 161
           K+    +++   L  S   +P EEL  S      +E  + + +   GR ++S    D + 
Sbjct: 101 KL----MEVSVKLEQSLSQIPYEELDISDEVREQVELVLSQFRRAKGRVDVSD---DELY 160

Query: 162 NQAEGIAPPSSD------VLVKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAED 221
              + +   SSD      VL ++A  L L    ++  E+VAL ++      A   G+  +
Sbjct: 161 EDLQSLCNKSSDVDAYQPVLERVAKKLHLMEIPDLAQESVALHEM-----VASSGGDVGE 220

Query: 222 IDQMVALVTRMHERLLTIK-----------QSQSSGPVS---------IPTDFCCPLSLE 281
             + +A+V +M +  +  +            S+S+G  S         IP DF CP+SLE
Sbjct: 221 NIEEMAMVLKMIKDFVQTEDDNGEEQKVGVNSRSNGQTSTAASQKIPVIPDDFRCPISLE 280

Query: 282 LMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWCETNN 341
           +M DPVIV+SGQTYER  I+ WI+ G + CPKT+Q L  T L PNY +++LIA WCE N+
Sbjct: 281 MMRDPVIVSSGQTYERTCIEKWIEGGHSTCPKTQQALTSTTLTPNYVLRSLIAQWCEAND 340

Query: 342 VKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFSEDSLSNDA 401
           ++   P                     P S R                            
Sbjct: 341 IEPPKP---------------------PSSLRP--------------------------- 400

Query: 402 GDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESHSHNRKAST 461
                                                                  RK S+
Sbjct: 401 -------------------------------------------------------RKVSS 460

Query: 462 SSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNHIEPELPPR 521
            S+ + A                                           N IE      
Sbjct: 461 FSSPAEA-------------------------------------------NKIE------ 520

Query: 522 LADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSSSLETLRGA 581
                   ++MW                                    L   + E  R A
Sbjct: 521 --------DLMW-----------------------------------RLAYGNPEDQRSA 580

Query: 582 TAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSINDNNKNAIA 641
             E+RLLAK N DNR+ IA+ GAI  LV LL + DS+IQE++VTALLNLSI +NNK AI 
Sbjct: 581 AGEIRLLAKRNADNRVAIAEAGAIPLLVGLLSTPDSRIQEHSVTALLNLSICENNKGAIV 624

Query: 642 QANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVELLGIGTPRG 701
            A AI  ++ VLK GS EA+EN+AATLFSLSVI+ENKV IG  GAI PLV LL  GT RG
Sbjct: 641 SAGAIPGIVQVLKKGSMEARENAAATLFSLSVIDENKVTIGALGAIPPLVVLLNEGTQRG 624

Query: 702 KKDAATALFNLSIFHENKARIVQAGAVRHLVELM-DPAAGMVDKAVAVLANLATIPEGRS 761
           KKDAATALFNL I+  NK + ++AG +  L  L+ +P +GMVD+A+A+LA L++ PEG++
Sbjct: 701 KKDAATALFNLCIYQGNKGKAIRAGVIPTLTRLLTEPGSGMVDEALAILAILSSHPEGKA 624

Query: 762 AIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPLVALSQSG 800
            IG    +P LVE +  GS R +ENAAA L+ LC+   +H     + G + PL+ L+ +G
Sbjct: 761 IIGSSDAVPSLVEFIRTGSPRNRENAAAVLVHLCSGDPQHLVEAQKLGLMGPLIDLAGNG 624

BLAST of Carg25148 vs. ExPASy Swiss-Prot
Match: Q5VRH9 (U-box domain-containing protein 12 OS=Oryza sativa subsp. japonica OX=39947 GN=PUB12 PE=2 SV=1)

HSP 1 Score: 279.6 bits (714), Expect = 1.1e-73
Identity = 210/651 (32.26%), Postives = 301/651 (46.24%), Query Frame = 0

Query: 162 PPSSDVLVKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAED-IDQMVALVTRMH 221
           P    +L +++  L L +  ++  E++AL  +  S       GE +  +DQM +L+ ++ 
Sbjct: 147 PSDPALLTRISHKLQLHTMADMKNESIALHNMVIST-----AGEPDGCVDQMSSLLKKLK 206

Query: 222 ERLLTIKQSQSS-----------GPVSIPTDFCCPLSLELMTDPVIVASGQTYERVFIKN 281
           + ++T   +  +            P+ IP +F CP+SLELM DPVIV+SGQTYER  I+ 
Sbjct: 207 DCVVTEDHANDALTTRSASIKHRSPI-IPDEFRCPISLELMQDPVIVSSGQTYERSCIQK 266

Query: 282 WIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWCETNNVKLSDPSRSVLGSFESDTHRE 341
           W+D G   CPKT+Q L HT+L PN+ +K+LI+ WCE N ++L                  
Sbjct: 267 WLDSGHKTCPKTQQPLSHTSLTPNFVLKSLISQWCEANGIEL------------------ 326

Query: 342 PLFSHSPQSARSVLGGTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSA 401
                                                                       
Sbjct: 327 ------------------------------------------------------------ 386

Query: 402 KLEENGGDLIAKPSMSPSRTNVPNSSEDESHSHNRKASTSSAVSNANLSRGTSGEANESP 461
                                     +++ +S ++KA+ SS   +A L            
Sbjct: 387 -------------------------PKNKQNSRDKKAAKSSDYDHAGLV----------- 446

Query: 462 NVSTNLTGYGSDAAGESKSEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFAS 521
                                                                       
Sbjct: 447 ------------------------------------------------------------ 506

Query: 522 KITTPSANETRPDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQC 581
                                  ++  L+S + +  R A  E+RLLAK N++NRI IA+ 
Sbjct: 507 ----------------------SLMNRLRSGNQDEQRAAAGEIRLLAKRNVNNRICIAEA 566

Query: 582 GAIEYLVNLLLSADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKE 641
           GAI  LVNLL S+D + QE+AVTALLNLSI++NNK +I  ++AI  ++ VLKTGS E +E
Sbjct: 567 GAIPLLVNLLSSSDPRTQEHAVTALLNLSIHENNKASIVDSHAIPKIVEVLKTGSMETRE 595

Query: 642 NSAATLFSLSVIEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARI 701
           N+AATLFSLSV++ENKV IG +GAI PL+ LL  G+PRGKKDAATA+FNL I+  NK R 
Sbjct: 627 NAAATLFSLSVVDENKVTIGAAGAIPPLINLLCDGSPRGKKDAATAIFNLCIYQGNKVRA 595

Query: 702 VQAGAVRHLVE-LMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSAR 761
           V+AG V HL+  L+DP  GM+D+A+++L+ LA  PEG+  I +   IP LVEV++ GS R
Sbjct: 687 VKAGIVIHLMNFLVDPTGGMIDEALSLLSILAGNPEGKIVIARSEPIPPLVEVIKTGSPR 595

Query: 762 GKENAAAALLQLCTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALL 800
            +ENAAA L  LC+  +         G    L  LS++GT RAK KA ++L
Sbjct: 747 NRENAAAILWLLCSADTEQTLAAKAAGVEDALKELSETGTDRAKRKASSIL 595

BLAST of Carg25148 vs. ExPASy TrEMBL
Match: A0A6J1G6Z2 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111451433 PE=4 SV=1)

HSP 1 Score: 1504.6 bits (3894), Expect = 0.0e+00
Identity = 809/814 (99.39%), Postives = 810/814 (99.51%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQ EALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQGEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELS SSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSLSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE-S 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLI KPSMSPSRTNVPNSSEDE S
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLITKPSMSPSRTNVPNSSEDEQS 420

Query: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480
           HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN
Sbjct: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480

Query: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540
           HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS
Sbjct: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI
Sbjct: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600

Query: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660
           NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKV+IGRSGAIGPLVE
Sbjct: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVRIGRSGAIGPLVE 660

Query: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720
           LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL
Sbjct: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720

Query: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780
           ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP
Sbjct: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780

Query: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814

BLAST of Carg25148 vs. ExPASy TrEMBL
Match: A0A6J1L1P2 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499603 PE=4 SV=1)

HSP 1 Score: 1484.2 bits (3841), Expect = 0.0e+00
Identity = 801/814 (98.40%), Postives = 806/814 (99.02%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLKLLLRHISSFFQLSSSDYI+LQPTLKYYHKIEG  KLLKPILDAVVDSDIASDE
Sbjct: 1   MGVSLLKLLLRHISSFFQLSSSDYIHLQPTLKYYHKIEGGLKLLKPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           ELIKAFEELDHSVDELRVLFENWQPLSSKVYL L+SEALITKIGKSSLDIFQLLRSSNEN
Sbjct: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLALRSEALITKIGKSSLDIFQLLRSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSSSSLEH VQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSSSLEHSVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVT MHERL+TIKQSQSSGPVSIPTD
Sbjct: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTCMHERLITIKQSQSSGPVSIPTD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS
Sbjct: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE-S 420
           EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDE S
Sbjct: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDEQS 420

Query: 421 HSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480
           H HNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN
Sbjct: 421 HIHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPN 480

Query: 481 HIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKS 540
           HIEPELPPRLADSRSRGNMMWLRPSERF+SKITT SANETRPDISAIEAQVRKVVEELKS
Sbjct: 481 HIEPELPPRLADSRSRGNMMWLRPSERFSSKITTLSANETRPDISAIEAQVRKVVEELKS 540

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIE+LVNLLLSADSKIQENAVTALLNLSI
Sbjct: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEHLVNLLLSADSKIQENAVTALLNLSI 600

Query: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660
           NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE
Sbjct: 601 NDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLVE 660

Query: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720
           LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL
Sbjct: 661 LLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANL 720

Query: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780
           ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP
Sbjct: 721 ATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPP 780

Query: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 LVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814

BLAST of Carg25148 vs. ExPASy TrEMBL
Match: A0A6J1D757 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111017892 PE=4 SV=1)

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 728/838 (86.87%), Postives = 769/838 (91.77%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSL+K+LLR ISSF QLSSSDY+NLQPTLKYYH IEGV KLL+PILDAVVDSDIASDE
Sbjct: 40  MGVSLIKVLLRRISSFLQLSSSDYVNLQPTLKYYHIIEGVLKLLRPILDAVVDSDIASDE 99

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           EL KAFE+LDHSVDELR+LFENWQ LSSKVY VLQSEALI+KIGKSSLDIFQLL+SSNEN
Sbjct: 100 ELTKAFEQLDHSVDELRMLFENWQLLSSKVYFVLQSEALISKIGKSSLDIFQLLQSSNEN 159

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSS+SLEHCVQK+ HIG+E+ISS+IKD IRNQ EGI  PSSDVLVKLADSLSLRSN
Sbjct: 160 LPEELSSTSLEHCVQKVNHIGKEQISSIIKDTIRNQIEGIG-PSSDVLVKLADSLSLRSN 219

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKE+AEQAE TGEAEDIDQMVALVTRMHERL+TIKQSQSS PVSIP D
Sbjct: 220 QEILIEAVALEKLKENAEQAENTGEAEDIDQMVALVTRMHERLITIKQSQSSSPVSIPAD 279

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWID+GLNVCPKTRQTL HTNLIPN+TVKALI
Sbjct: 280 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDLGLNVCPKTRQTLAHTNLIPNFTVKALI 339

Query: 301 ANWCETNNVKLSDPSRSV---------LGSFESDTHREPLFSH-------SPQSARSV-- 360
            NWCETNNVKLSDP+RSV         +G+F+SD HREP+F         SPQS RS   
Sbjct: 340 ENWCETNNVKLSDPARSVNLNQLSPLLVGNFDSDAHREPIFPRSPGYQLMSPQSTRSAGS 399

Query: 361 ------LGGTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGG 420
                 LGGTHRDG+SSLLSHSFSEDSLSNDAGDERGID SRL LTS+ED+  KLEENG 
Sbjct: 400 GKNLNSLGGTHRDGNSSLLSHSFSEDSLSNDAGDERGIDFSRLRLTSSEDRPGKLEENGC 459

Query: 421 DLIAKPSMSPSRTNVPNSSEDE-SHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNL 480
           DL+AKPSMSPSRTNVPN+SEDE SHSHNR ASTSSA+SNANLSR TSGEANE+  +STNL
Sbjct: 460 DLVAKPSMSPSRTNVPNTSEDEHSHSHNRSASTSSAISNANLSRETSGEANEASQLSTNL 519

Query: 481 TGYGSDAAGESKSEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPS 540
           TGYGSDAAGESKSEP AA +ST +  EPE+P RLADSRSRGN +WLRPSERFA++IT  S
Sbjct: 520 TGYGSDAAGESKSEPSAATTSTTSRREPEIPARLADSRSRGNTIWLRPSERFATRITASS 579

Query: 541 ANETRPDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYL 600
             ETRPD+SAIEAQV+KVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAI YL
Sbjct: 580 TTETRPDLSAIEAQVQKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIVYL 639

Query: 601 VNLLLSADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATL 660
           V+LLLSADSKIQENAVTALLNLSINDNNK+AIAQANAIEPLIHVLKTGSPEAKENSAATL
Sbjct: 640 VDLLLSADSKIQENAVTALLNLSINDNNKSAIAQANAIEPLIHVLKTGSPEAKENSAATL 699

Query: 661 FSLSVIEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAV 720
           FSLSVIEENKVKIGRSGAIGPLVELLG GTPRGKKDAATALFNLSIFHENKARIVQAGAV
Sbjct: 700 FSLSVIEENKVKIGRSGAIGPLVELLGNGTPRGKKDAATALFNLSIFHENKARIVQAGAV 759

Query: 721 RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA 780
           RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA
Sbjct: 760 RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA 819

Query: 781 ALLQLCTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           ALLQLCTTS+RHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 820 ALLQLCTTSNRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 876

BLAST of Carg25148 vs. ExPASy TrEMBL
Match: A0A6J1D5U0 (RING-type E3 ubiquitin transferase OS=Momordica charantia OX=3673 GN=LOC111017892 PE=4 SV=1)

HSP 1 Score: 1336.6 bits (3458), Expect = 0.0e+00
Identity = 728/838 (86.87%), Postives = 769/838 (91.77%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSL+K+LLR ISSF QLSSSDY+NLQPTLKYYH IEGV KLL+PILDAVVDSDIASDE
Sbjct: 1   MGVSLIKVLLRRISSFLQLSSSDYVNLQPTLKYYHIIEGVLKLLRPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           EL KAFE+LDHSVDELR+LFENWQ LSSKVY VLQSEALI+KIGKSSLDIFQLL+SSNEN
Sbjct: 61  ELTKAFEQLDHSVDELRMLFENWQLLSSKVYFVLQSEALISKIGKSSLDIFQLLQSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSS+SLEHCVQK+ HIG+E+ISS+IKD IRNQ EGI  PSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSTSLEHCVQKVNHIGKEQISSIIKDTIRNQIEGIG-PSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEILIEAVALEKLKE+AEQAE TGEAEDIDQMVALVTRMHERL+TIKQSQSS PVSIP D
Sbjct: 181 QEILIEAVALEKLKENAEQAENTGEAEDIDQMVALVTRMHERLITIKQSQSSSPVSIPAD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWID+GLNVCPKTRQTL HTNLIPN+TVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDLGLNVCPKTRQTLAHTNLIPNFTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSV---------LGSFESDTHREPLFSH-------SPQSARSV-- 360
            NWCETNNVKLSDP+RSV         +G+F+SD HREP+F         SPQS RS   
Sbjct: 301 ENWCETNNVKLSDPARSVNLNQLSPLLVGNFDSDAHREPIFPRSPGYQLMSPQSTRSAGS 360

Query: 361 ------LGGTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGG 420
                 LGGTHRDG+SSLLSHSFSEDSLSNDAGDERGID SRL LTS+ED+  KLEENG 
Sbjct: 361 GKNLNSLGGTHRDGNSSLLSHSFSEDSLSNDAGDERGIDFSRLRLTSSEDRPGKLEENGC 420

Query: 421 DLIAKPSMSPSRTNVPNSSEDE-SHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNL 480
           DL+AKPSMSPSRTNVPN+SEDE SHSHNR ASTSSA+SNANLSR TSGEANE+  +STNL
Sbjct: 421 DLVAKPSMSPSRTNVPNTSEDEHSHSHNRSASTSSAISNANLSRETSGEANEASQLSTNL 480

Query: 481 TGYGSDAAGESKSEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPS 540
           TGYGSDAAGESKSEP AA +ST +  EPE+P RLADSRSRGN +WLRPSERFA++IT  S
Sbjct: 481 TGYGSDAAGESKSEPSAATTSTTSRREPEIPARLADSRSRGNTIWLRPSERFATRITASS 540

Query: 541 ANETRPDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYL 600
             ETRPD+SAIEAQV+KVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAI YL
Sbjct: 541 TTETRPDLSAIEAQVQKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIVYL 600

Query: 601 VNLLLSADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATL 660
           V+LLLSADSKIQENAVTALLNLSINDNNK+AIAQANAIEPLIHVLKTGSPEAKENSAATL
Sbjct: 601 VDLLLSADSKIQENAVTALLNLSINDNNKSAIAQANAIEPLIHVLKTGSPEAKENSAATL 660

Query: 661 FSLSVIEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAV 720
           FSLSVIEENKVKIGRSGAIGPLVELLG GTPRGKKDAATALFNLSIFHENKARIVQAGAV
Sbjct: 661 FSLSVIEENKVKIGRSGAIGPLVELLGNGTPRGKKDAATALFNLSIFHENKARIVQAGAV 720

Query: 721 RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA 780
           RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA
Sbjct: 721 RHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAA 780

Query: 781 ALLQLCTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           ALLQLCTTS+RHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG
Sbjct: 781 ALLQLCTTSNRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 837

BLAST of Carg25148 vs. ExPASy TrEMBL
Match: A0A0A0LPT2 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G277080 PE=4 SV=1)

HSP 1 Score: 1330.5 bits (3442), Expect = 0.0e+00
Identity = 737/842 (87.53%), Postives = 766/842 (90.97%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           MGVSLLK+LLRHISSF QLSSSDYINLQPTLKYYHKIEG  KLL+PILDAVVDSDIASDE
Sbjct: 1   MGVSLLKVLLRHISSFLQLSSSDYINLQPTLKYYHKIEGALKLLRPILDAVVDSDIASDE 60

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
           EL +AFEELDHSVDELRVLFENWQPLSSKVY VLQSE LI+KIGK SLDIFQLL+SSNEN
Sbjct: 61  ELTQAFEELDHSVDELRVLFENWQPLSSKVYFVLQSETLISKIGKFSLDIFQLLQSSNEN 120

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LPEELSS SLEHCVQKIK+IG+EEISSVIKDAIRNQ +GIA PSSDVLVKLADSLSLRSN
Sbjct: 121 LPEELSSKSLEHCVQKIKNIGKEEISSVIKDAIRNQVDGIA-PSSDVLVKLADSLSLRSN 180

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           Q ILIEAVALEKLKESAEQAE TGEAEDIDQM+ LVTRMHERL+ IKQSQSS PVSIP D
Sbjct: 181 QAILIEAVALEKLKESAEQAENTGEAEDIDQMIGLVTRMHERLIMIKQSQSSSPVSIPPD 240

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           FCCPLSLELMTDPVIVASGQTYERVFIKNWID GLNVCPKTRQTLVHTNLIPNYTVKALI
Sbjct: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDQGLNVCPKTRQTLVHTNLIPNYTVKALI 300

Query: 301 ANWCETNNVKLSDPSRSV---------LGSFESDTHREPLFSH-------SPQSARSV-- 360
           ANWC+TNNVKLSDPS+SV         +GSFE DTHREPLF H       SPQS RS   
Sbjct: 301 ANWCDTNNVKLSDPSKSVNLNQISPLLVGSFEPDTHREPLFPHSPGYQPMSPQSTRSAGS 360

Query: 361 ------LGGTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGG 420
                 LGGTHRDGSSSLL HS SEDSLSNDAGDE  I+V RLLL+S+EDQ AKLEENG 
Sbjct: 361 GKNSNSLGGTHRDGSSSLLPHSLSEDSLSNDAGDEGAIEVDRLLLSSSEDQMAKLEENGC 420

Query: 421 DLIAKPSMSPSRTNVPNS-SEDE-SHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTN 480
           D +AKPSMSPSRTNV NS  EDE SHSHNR +STSS VSNAN SRGTSGEANE+ ++STN
Sbjct: 421 DPVAKPSMSPSRTNVLNSCGEDEPSHSHNRSSSTSSGVSNANHSRGTSGEANEATHLSTN 480

Query: 481 LTGYGSDAAGESKSEPLAAASSTPNHIEPEL--PPRLAD-SRSRGNMMWLRPSERFASKI 540
           LTGYGSDAAGESKSEPLAAA+ T NH EPE   PPRLAD  R RGN MWLRPSERFAS+I
Sbjct: 481 LTGYGSDAAGESKSEPLAAATPTTNHREPEREHPPRLADHPRPRGNTMWLRPSERFASRI 540

Query: 541 TTPSANETRPDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGA 600
            T SANETRPD+SAIEAQV+KVVEELKSSSL+TLRGATAELRLLAKHNMDNRIVIAQCGA
Sbjct: 541 ITSSANETRPDLSAIEAQVQKVVEELKSSSLDTLRGATAELRLLAKHNMDNRIVIAQCGA 600

Query: 601 IEYLVNLLLSADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENS 660
           I+YLV LLLS DSKIQENAVTALLNLSINDNNK+AIAQANAIEPLIHVLKTGSPEAKENS
Sbjct: 601 IDYLVGLLLSEDSKIQENAVTALLNLSINDNNKSAIAQANAIEPLIHVLKTGSPEAKENS 660

Query: 661 AATLFSLSVIEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQ 720
           AATLFSLSVIEENKVKIGRSGAIGPLVELLG GTPRGKKDAATALFNLSIFHENKARIVQ
Sbjct: 661 AATLFSLSVIEENKVKIGRSGAIGPLVELLGNGTPRGKKDAATALFNLSIFHENKARIVQ 720

Query: 721 AGAVRHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKE 780
           AGAVRHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKE
Sbjct: 721 AGAVRHLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKE 780

Query: 781 NAAAALLQLCTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSG 814
           NAAAALLQLCTTS+RHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSG
Sbjct: 781 NAAAALLQLCTTSNRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSG 840

BLAST of Carg25148 vs. TAIR 10
Match: AT2G23140.1 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 863.2 bits (2229), Expect = 1.6e-250
Identity = 509/833 (61.10%), Postives = 606/833 (72.75%), Query Frame = 0

Query: 6   LKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDEELIKA 65
           +++LLR ISSF  LSSS +I+L P  KYY ++E + ++LKPI D VV SD   DE+L KA
Sbjct: 4   MEVLLRSISSFLNLSSSKHIDLDPFEKYYKRVEELLRVLKPIADVVVTSDFVFDEKLGKA 63

Query: 66  FEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNENLPEEL 125
           FEEL   VD+   LF +WQ  SSKVY VLQ E+L+ K+  + +D FQ L SS  +LP+EL
Sbjct: 64  FEELTQDVDQSIDLFRSWQAFSSKVYFVLQIESLLPKMRDTIVDTFQFLMSSKNHLPDEL 123

Query: 126 SSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSNQEILI 185
           S +SLE C++KIKH+  EEISSVI  A+R+Q +G+  PS ++LVK+ ++  LRSNQEILI
Sbjct: 124 SPASLEQCLEKIKHLSYEEISSVIDGALRDQRDGVG-PSPEILVKIGENTGLRSNQEILI 183

Query: 186 EAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTDFCCPL 245
           EAVALE+ KE AEQ+E   E E +DQ++ +V RMHERLL IKQ+Q+S  V+I  DF CPL
Sbjct: 184 EAVALERQKEMAEQSENNAEVEFLDQLIVIVNRMHERLLLIKQTQTSS-VAILADFFCPL 243

Query: 246 SLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWCE 305
           SLE+MTDPVIV+SGQTYE+ FIK WID+GL VCPKTRQTL HT LIPNYTVKALIANWCE
Sbjct: 244 SLEVMTDPVIVSSGQTYEKAFIKRWIDLGLKVCPKTRQTLTHTTLIPNYTVKALIANWCE 303

Query: 306 TNNVKLSDPSRSV----LGSFESDTHREPLF------------SHSPQSARSVLG----- 365
           TN+VKL DP++S     L    S T   P              SH   ++ S  G     
Sbjct: 304 TNDVKLPDPNKSTSLNELSPLLSCTDSIPSTGADVSARKVSNKSHDWDASSSETGKPSFS 363

Query: 366 --GTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKP 425
              T R+G+S     S    S    +G+  G+D  R  L   ED+S    E   D   + 
Sbjct: 364 SRATEREGASPSRPASALGASSPGISGNGYGLDARRGSLNDFEDRSNDSRELRTDAPGRS 423

Query: 426 SM-SPSRTNVPNSSEDESHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSD 485
           S+ S +R +V N    E+H H+R  S +S VSN    R    +ANE+   S + T Y SD
Sbjct: 424 SVSSTTRGSVENGQTSENH-HHRSPSATSTVSNEEFPR---ADANENSEESAHATPYSSD 483

Query: 486 AAGESKSEPLAAASSTPNHIE-PELPPRLADSRSRGNMMWLRPSERFASKITTPSANETR 545
           A+GE +S PLAA +S     +  +  P+  D R+RG   W RPSER  S+I +  +NETR
Sbjct: 484 ASGEIRSGPLAATTSAATRRDLSDFSPKFMDRRTRG-QFWRRPSERLGSRIVSAPSNETR 543

Query: 546 PDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLL 605
            D+S +E QV+K+VEELKSSSL+T R ATAELRLLAKHNMDNRIVI   GAI  LV LL 
Sbjct: 544 RDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLY 603

Query: 606 SADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSV 665
           S DS  QENAVTALLNLSINDNNK AIA A AIEPLIHVL+ GS EAKENSAATLFSLSV
Sbjct: 604 STDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSV 663

Query: 666 IEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVE 725
           IEENK+KIG+SGAIGPLV+LLG GTPRGKKDAATALFNLSI  ENKA IVQ+GAVR+L++
Sbjct: 664 IEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLID 723

Query: 726 LMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQL 785
           LMDPAAGMVDKAVAVLANLATIPEGR+AIGQEGGIP+LVEVVELGSARGKENAAAALLQL
Sbjct: 724 LMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQL 783

Query: 786 CTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
            T S R C+MVLQEGAVPPLVALSQSGT RA+EKAQALLS+FR+QRHGN+GRG
Sbjct: 784 STNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGNAGRG 829

BLAST of Carg25148 vs. TAIR 10
Match: AT2G23140.2 (RING/U-box superfamily protein with ARM repeat domain )

HSP 1 Score: 863.2 bits (2229), Expect = 1.6e-250
Identity = 509/833 (61.10%), Postives = 606/833 (72.75%), Query Frame = 0

Query: 6   LKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDEELIKA 65
           +++LLR ISSF  LSSS +I+L P  KYY ++E + ++LKPI D VV SD   DE+L KA
Sbjct: 1   MEVLLRSISSFLNLSSSKHIDLDPFEKYYKRVEELLRVLKPIADVVVTSDFVFDEKLGKA 60

Query: 66  FEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNENLPEEL 125
           FEEL   VD+   LF +WQ  SSKVY VLQ E+L+ K+  + +D FQ L SS  +LP+EL
Sbjct: 61  FEELTQDVDQSIDLFRSWQAFSSKVYFVLQIESLLPKMRDTIVDTFQFLMSSKNHLPDEL 120

Query: 126 SSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSNQEILI 185
           S +SLE C++KIKH+  EEISSVI  A+R+Q +G+  PS ++LVK+ ++  LRSNQEILI
Sbjct: 121 SPASLEQCLEKIKHLSYEEISSVIDGALRDQRDGVG-PSPEILVKIGENTGLRSNQEILI 180

Query: 186 EAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTDFCCPL 245
           EAVALE+ KE AEQ+E   E E +DQ++ +V RMHERLL IKQ+Q+S  V+I  DF CPL
Sbjct: 181 EAVALERQKEMAEQSENNAEVEFLDQLIVIVNRMHERLLLIKQTQTSS-VAILADFFCPL 240

Query: 246 SLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWCE 305
           SLE+MTDPVIV+SGQTYE+ FIK WID+GL VCPKTRQTL HT LIPNYTVKALIANWCE
Sbjct: 241 SLEVMTDPVIVSSGQTYEKAFIKRWIDLGLKVCPKTRQTLTHTTLIPNYTVKALIANWCE 300

Query: 306 TNNVKLSDPSRSV----LGSFESDTHREPLF------------SHSPQSARSVLG----- 365
           TN+VKL DP++S     L    S T   P              SH   ++ S  G     
Sbjct: 301 TNDVKLPDPNKSTSLNELSPLLSCTDSIPSTGADVSARKVSNKSHDWDASSSETGKPSFS 360

Query: 366 --GTHRDGSSSLLSHSFSEDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKP 425
              T R+G+S     S    S    +G+  G+D  R  L   ED+S    E   D   + 
Sbjct: 361 SRATEREGASPSRPASALGASSPGISGNGYGLDARRGSLNDFEDRSNDSRELRTDAPGRS 420

Query: 426 SM-SPSRTNVPNSSEDESHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSD 485
           S+ S +R +V N    E+H H+R  S +S VSN    R    +ANE+   S + T Y SD
Sbjct: 421 SVSSTTRGSVENGQTSENH-HHRSPSATSTVSNEEFPR---ADANENSEESAHATPYSSD 480

Query: 486 AAGESKSEPLAAASSTPNHIE-PELPPRLADSRSRGNMMWLRPSERFASKITTPSANETR 545
           A+GE +S PLAA +S     +  +  P+  D R+RG   W RPSER  S+I +  +NETR
Sbjct: 481 ASGEIRSGPLAATTSAATRRDLSDFSPKFMDRRTRG-QFWRRPSERLGSRIVSAPSNETR 540

Query: 546 PDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLL 605
            D+S +E QV+K+VEELKSSSL+T R ATAELRLLAKHNMDNRIVI   GAI  LV LL 
Sbjct: 541 RDLSEVETQVKKLVEELKSSSLDTQRQATAELRLLAKHNMDNRIVIGNSGAIVLLVELLY 600

Query: 606 SADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSV 665
           S DS  QENAVTALLNLSINDNNK AIA A AIEPLIHVL+ GS EAKENSAATLFSLSV
Sbjct: 601 STDSATQENAVTALLNLSINDNNKKAIADAGAIEPLIHVLENGSSEAKENSAATLFSLSV 660

Query: 666 IEENKVKIGRSGAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVE 725
           IEENK+KIG+SGAIGPLV+LLG GTPRGKKDAATALFNLSI  ENKA IVQ+GAVR+L++
Sbjct: 661 IEENKIKIGQSGAIGPLVDLLGNGTPRGKKDAATALFNLSIHQENKAMIVQSGAVRYLID 720

Query: 726 LMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQL 785
           LMDPAAGMVDKAVAVLANLATIPEGR+AIGQEGGIP+LVEVVELGSARGKENAAAALLQL
Sbjct: 721 LMDPAAGMVDKAVAVLANLATIPEGRNAIGQEGGIPLLVEVVELGSARGKENAAAALLQL 780

Query: 786 CTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
            T S R C+MVLQEGAVPPLVALSQSGT RA+EKAQALLS+FR+QRHGN+GRG
Sbjct: 781 STNSGRFCNMVLQEGAVPPLVALSQSGTPRAREKAQALLSYFRNQRHGNAGRG 826

BLAST of Carg25148 vs. TAIR 10
Match: AT5G67340.1 (ARM repeat superfamily protein )

HSP 1 Score: 644.8 bits (1662), Expect = 9.2e-185
Identity = 398/815 (48.83%), Postives = 514/815 (63.07%), Query Frame = 0

Query: 1   MGVSLLKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDE 60
           M VS L++LL +ISS+  LSS D ++  P  KYY + E + KL+KP+L+ ++DSD A  E
Sbjct: 5   MEVSWLRVLLDNISSYLSLSSMDDLSSNPAHKYYTRGEDIGKLIKPVLENLIDSDAAPSE 64

Query: 61  ELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNEN 120
            L   FEEL   VDELR  F++WQPLS++++ VL+ E+L +K+ +SSL++FQLL+   ++
Sbjct: 65  LLNNGFEELAQYVDELREQFQSWQPLSTRIFYVLRIESLASKLRESSLEVFQLLKHCEQH 124

Query: 121 LPEELSSSSLEHCVQKIKHIGREEISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSN 180
           LP +L S S E C++ +K + R+EIS  I  A+++Q +G+  P+S+VLVK+A+S  LRSN
Sbjct: 125 LPADLISPSFEECIELVKLVARDEISYTIDQALKDQKKGVG-PTSEVLVKIAESTGLRSN 184

Query: 181 QEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTD 240
           QEIL+E V L  +KE AE  +   EAE +D +++L T+MHE L  IKQ+Q   PV +P+D
Sbjct: 185 QEILVEGVVLTNMKEDAELTDNDTEAEYLDGLISLTTQMHEYLSDIKQAQLRCPVRVPSD 244

Query: 241 FCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALI 300
           F C LSLELMTDPVIVASGQT+ERVFI+ WIDMGL VCPKTRQ L HT L PN+ V+A +
Sbjct: 245 FRCSLSLELMTDPVIVASGQTFERVFIQKWIDMGLMVCPKTRQALSHTTLTPNFIVRAFL 304

Query: 301 ANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFS 360
           A+WCETNNV   DP   +        H    F    +S R+         SSS   HS S
Sbjct: 305 ASWCETNNVYPPDPLELI--------HSSEPFPLLVESVRA---------SSSENGHSES 364

Query: 361 EDSLSNDAGDERGIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESH 420
            D                                                    +E+   
Sbjct: 365 LD----------------------------------------------------AEELRQ 424

Query: 421 SHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNH 480
             +R AS    V                           S+   ++K    AAA      
Sbjct: 425 VFSRSASAPGIV---------------------------SEVVCKTKRNNNAAADR---- 484

Query: 481 IEPELPPRLADSRSRGNMMWLRPSER-FASKITTPSANETRPDISAIEAQVRKVVEELKS 540
                      S +R N  W  P ER +      P+        S+IE +V+K++++LKS
Sbjct: 485 -----------SLTRSNTPWKFPEERHWRHPGIIPATVRETGSSSSIETEVKKLIDDLKS 544

Query: 541 SSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSI 600
           SSL+T R ATA +R+LA+++ DNRIVIA+C AI  LV+LL S D +IQ +AVT LLNLSI
Sbjct: 545 SSLDTQREATARIRILARNSTDNRIVIARCEAIPSLVSLLYSTDERIQADAVTCLLNLSI 604

Query: 601 NDNNKNAIAQANAIEPLIHVLKTG-SPEAKENSAATLFSLSVIEENKVKIGRSGAIGPLV 660
           NDNNK+ IA++ AI PLIHVLKTG   EAK NSAATLFSLSVIEE K +IG +GAI PLV
Sbjct: 605 NDNNKSLIAESGAIVPLIHVLKTGYLEEAKANSAATLFSLSVIEEYKTEIGEAGAIEPLV 664

Query: 661 ELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLAN 720
           +LLG G+  GKKDAATALFNLSI HENK ++++AGAVR+LVELMDPA GMV+KAV VLAN
Sbjct: 665 DLLGSGSLSGKKDAATALFNLSIHHENKTKVIEAGAVRYLVELMDPAFGMVEKAVVVLAN 707

Query: 721 LATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVP 780
           LAT+ EG+ AIG+EGGIPVLVEVVELGSARGKENA AALLQLCT S + C+ V++EG +P
Sbjct: 725 LATVREGKIAIGEEGGIPVLVEVVELGSARGKENATAALLQLCTHSPKFCNNVIREGVIP 707

Query: 781 PLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           PLVAL++SGTAR KEKAQ LL +F++ R  N  RG
Sbjct: 785 PLVALTKSGTARGKEKAQNLLKYFKAHRQSNQRRG 707

BLAST of Carg25148 vs. TAIR 10
Match: AT3G54790.1 (ARM repeat superfamily protein )

HSP 1 Score: 499.2 bits (1284), Expect = 6.2e-141
Identity = 338/811 (41.68%), Postives = 477/811 (58.82%), Query Frame = 0

Query: 6   LKLLLRHISSFFQLSSSDYINLQPTLKYYHKIEGVFKLLKPILDAVVDSDIASDEELIKA 65
           ++ LL  IS +  L +   I   P       +  + KLLKP+LD VVD  I SD+ L K 
Sbjct: 6   VRCLLNSISRYLHLVACQTIRFNPIQTCIGNMVLLLKLLKPLLDEVVDCKIPSDDCLYKG 65

Query: 66  FEELDHSVDELRVLFENWQPLSSKVYLVLQSEALITKIGKSSLDIFQLLRSSNENLPEEL 125
            E+LD  V++ R   E+W P  SK++ V Q E L+ K+   SL+I ++L   +++ P   
Sbjct: 66  CEDLDSVVNQAREFLEDWSPKLSKLFGVFQCEVLLGKVQTCSLEISRILLQLSQSSPVTS 125

Query: 126 SSSSLEHCVQKIKHIGRE-EISSVIKDAIRNQAEGIAPPSSDVLVKLADSLSLRSNQEIL 185
           S  S+E CVQ+ +   +E  +  ++++A+RNQ + I    ++ L  +   L L SNQ++L
Sbjct: 126 SVQSVERCVQETESFKQEGTLMELMENALRNQKDDITSLDNNHLESIIQMLGLISNQDLL 185

Query: 186 IEAVALEKLKESAEQAEQTGEAEDIDQMVALVTRMHERLLTIKQSQSSGPVSIPTDFCCP 245
            E++ +EK +  ++ ++   + E  +Q++ LV  + E +L  +  + +  +SIP  F CP
Sbjct: 186 KESITVEKERIRSQASKSEEDMEQTEQLIELVLCIREHMLKTEFLEVAKGISIPPYFRCP 245

Query: 246 LSLELMTDPVIVASGQTYERVFIKNWIDMGLNVCPKTRQTLVHTNLIPNYTVKALIANWC 305
           LS ELM DPVIVASGQT++R  IK W+D GL VCP+TRQ L H  LIPNYTVKA+IA+W 
Sbjct: 246 LSTELMLDPVIVASGQTFDRTSIKKWLDNGLAVCPRTRQVLTHQELIPNYTVKAMIASWL 305

Query: 306 ETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQSARSVLGGTHRDGSSSLLSHSFSEDSL 365
           E N + L+  S       ++ +    + S       S         SSSL S S    SL
Sbjct: 306 EANRINLATNSCHQYDGGDASSMANNMGSQDFNRTESFRFSLR---SSSLTSRS----SL 365

Query: 366 SNDAGDER-GIDVSRLLLTSTEDQSAKLEENGGDLIAKPSMSPSRTNVPNSSEDESHSHN 425
               G E+  I+VS  L   ++ +  ++ E          +SP           +S++H+
Sbjct: 366 ETGNGFEKLKINVSASLCGESQSKDLEIFE---------LLSPG----------QSYTHS 425

Query: 426 RKASTSSAVSNANLSRGTSGEANESPNVSTNLTGYGSDAAGESKSEPLAAASSTPNHIEP 485
           R  S  S VS+ +         +E+ ++  N       ++ E   +    +S+  NH   
Sbjct: 426 RSESVCSVVSSVDY---VPSVTHETESILGN-----HQSSSEMSPKKNLESSNNVNH--- 485

Query: 486 ELPPRLADSRSRGNMMWLRPSERFASKITTPSANETRPDISAIEAQVRKVVEELKSSSLE 545
                                E  A+K    S ++     +   +   K+VE+LKS S +
Sbjct: 486 ---------------------EHSAAKTYECSVHDLDDSGTMTTSHTIKLVEDLKSGSNK 545

Query: 546 TLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVNLLLSADSKIQENAVTALLNLSINDNN 605
               A AE+R L  ++++NR+ I +CGAI  L++LL S +   QE+AVTALLNLSI++ N
Sbjct: 546 VKTAAAAEIRHLTINSIENRVHIGRCGAITPLLSLLYSEEKLTQEHAVTALLNLSISELN 605

Query: 606 KNAIAQANAIEPLIHVLKTGSPEAKENSAATLFSLSVIEENKVKIGRS-GAIGPLVELLG 665
           K  I +  AIEPL+HVL TG+  AKENSAA+LFSLSV++ N+ +IG+S  AI  LV LLG
Sbjct: 606 KAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFSLSVLQVNRERIGQSNAAIQALVNLLG 665

Query: 666 IGTPRGKKDAATALFNLSIFHENKARIVQAGAVRHLVELMDPAAGMVDKAVAVLANLATI 725
            GT RGKKDAA+ALFNLSI H+NKARIVQA AV++LVEL+DP   MVDKAVA+LANL+ +
Sbjct: 666 KGTFRGKKDAASALFNLSITHDNKARIVQAKAVKYLVELLDPDLEMVDKAVALLANLSAV 725

Query: 726 PEGRSAIGQEGGIPVLVEVVELGSARGKENAAAALLQLCTTSSRHCSMVLQEGAVPPLVA 785
            EGR AI +EGGIP+LVE V+LGS RGKENAA+ LLQLC  S + C++VLQEGA+PPLVA
Sbjct: 726 GEGRQAIVREGGIPLLVETVDLGSQRGKENAASVLLQLCLNSPKFCTLVLQEGAIPPLVA 758

Query: 786 LSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LSQSGT RAKEKAQ LLSHFR+QR     +G
Sbjct: 786 LSQSGTQRAKEKAQQLLSHFRNQRDARMKKG 758

BLAST of Carg25148 vs. TAIR 10
Match: AT3G54790.2 (ARM repeat superfamily protein )

HSP 1 Score: 494.2 bits (1271), Expect = 2.0e-139
Identity = 331/777 (42.60%), Postives = 465/777 (59.85%), Query Frame = 0

Query: 40  VFKLLKPILDAVVDSDIASDEELIKAFEELDHSVDELRVLFENWQPLSSKVYLVLQSEAL 99
           + KLLKP+LD VVD  I SD+ L K  E+LD  V++ R   E+W P  SK++ V Q E L
Sbjct: 4   LLKLLKPLLDEVVDCKIPSDDCLYKGCEDLDSVVNQAREFLEDWSPKLSKLFGVFQCEVL 63

Query: 100 ITKIGKSSLDIFQLLRSSNENLPEELSSSSLEHCVQKIKHIGRE-EISSVIKDAIRNQAE 159
           + K+   SL+I ++L   +++ P   S  S+E CVQ+ +   +E  +  ++++A+RNQ +
Sbjct: 64  LGKVQTCSLEISRILLQLSQSSPVTSSVQSVERCVQETESFKQEGTLMELMENALRNQKD 123

Query: 160 GIAPPSSDVLVKLADSLSLRSNQEILIEAVALEKLKESAEQAEQTGEAEDIDQMVALVTR 219
            I    ++ L  +   L L SNQ++L E++ +EK +  ++ ++   + E  +Q++ LV  
Sbjct: 124 DITSLDNNHLESIIQMLGLISNQDLLKESITVEKERIRSQASKSEEDMEQTEQLIELVLC 183

Query: 220 MHERLLTIKQSQSSGPVSIPTDFCCPLSLELMTDPVIVASGQTYERVFIKNWIDMGLNVC 279
           + E +L  +  + +  +SIP  F CPLS ELM DPVIVASGQT++R  IK W+D GL VC
Sbjct: 184 IREHMLKTEFLEVAKGISIPPYFRCPLSTELMLDPVIVASGQTFDRTSIKKWLDNGLAVC 243

Query: 280 PKTRQTLVHTNLIPNYTVKALIANWCETNNVKLSDPSRSVLGSFESDTHREPLFSHSPQS 339
           P+TRQ L H  LIPNYTVKA+IA+W E N + L+  S       ++ +    + S     
Sbjct: 244 PRTRQVLTHQELIPNYTVKAMIASWLEANRINLATNSCHQYDGGDASSMANNMGSQDFNR 303

Query: 340 ARSVLGGTHRDGSSSLLSHSFSEDSLSNDAGDER-GIDVSRLLLTSTEDQSAKLEENGGD 399
             S         SSSL S S    SL    G E+  I+VS  L   ++ +  ++ E    
Sbjct: 304 TESFRFSLR---SSSLTSRS----SLETGNGFEKLKINVSASLCGESQSKDLEIFE---- 363

Query: 400 LIAKPSMSPSRTNVPNSSEDESHSHNRKASTSSAVSNANLSRGTSGEANESPNVSTNLTG 459
                 +SP           +S++H+R  S  S VS+ +         +E+ ++  N   
Sbjct: 364 -----LLSPG----------QSYTHSRSESVCSVVSSVDY---VPSVTHETESILGN--- 423

Query: 460 YGSDAAGESKSEPLAAASSTPNHIEPELPPRLADSRSRGNMMWLRPSERFASKITTPSAN 519
               ++ E   +    +S+  NH                        E  A+K    S +
Sbjct: 424 --HQSSSEMSPKKNLESSNNVNH------------------------EHSAAKTYECSVH 483

Query: 520 ETRPDISAIEAQVRKVVEELKSSSLETLRGATAELRLLAKHNMDNRIVIAQCGAIEYLVN 579
           +     +   +   K+VE+LKS S +    A AE+R L  ++++NR+ I +CGAI  L++
Sbjct: 484 DLDDSGTMTTSHTIKLVEDLKSGSNKVKTAAAAEIRHLTINSIENRVHIGRCGAITPLLS 543

Query: 580 LLLSADSKIQENAVTALLNLSINDNNKNAIAQANAIEPLIHVLKTGSPEAKENSAATLFS 639
           LL S +   QE+AVTALLNLSI++ NK  I +  AIEPL+HVL TG+  AKENSAA+LFS
Sbjct: 544 LLYSEEKLTQEHAVTALLNLSISELNKAMIVEVGAIEPLVHVLNTGNDRAKENSAASLFS 603

Query: 640 LSVIEENKVKIGRS-GAIGPLVELLGIGTPRGKKDAATALFNLSIFHENKARIVQAGAVR 699
           LSV++ N+ +IG+S  AI  LV LLG GT RGKKDAA+ALFNLSI H+NKARIVQA AV+
Sbjct: 604 LSVLQVNRERIGQSNAAIQALVNLLGKGTFRGKKDAASALFNLSITHDNKARIVQAKAVK 663

Query: 700 HLVELMDPAAGMVDKAVAVLANLATIPEGRSAIGQEGGIPVLVEVVELGSARGKENAAAA 759
           +LVEL+DP   MVDKAVA+LANL+ + EGR AI +EGGIP+LVE V+LGS RGKENAA+ 
Sbjct: 664 YLVELLDPDLEMVDKAVALLANLSAVGEGRQAIVREGGIPLLVETVDLGSQRGKENAASV 722

Query: 760 LLQLCTTSSRHCSMVLQEGAVPPLVALSQSGTARAKEKAQALLSHFRSQRHGNSGRG 814
           LLQLC  S + C++VLQEGA+PPLVALSQSGT RAKEKAQ LLSHFR+QR     +G
Sbjct: 724 LLQLCLNSPKFCTLVLQEGAIPPLVALSQSGTQRAKEKAQQLLSHFRNQRDARMKKG 722