Carg25113 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25113
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	NADH-u_ox-rdase domain-containing protein
Location	Carg_Chr13: 5379599 .. 5380206 (-)
RNA-Seq Expression	Carg25113
Synteny	Carg25113

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAGTCGCACAGCGAGGGAGAGAGCCGGCGGAGGTCAGTTTCAGCACCGGAGAACGTGATGAACACCGACATTACAGCTTCGACTAAGCCAGAGTATCCAGTAATAGATCGGAATCCTCCATTTACCAAAGTCGTTGGCAATTTCGACACTCTCGATTACCTCCGTTTCGTCACCATTACCGGCGTTTCCGTCACCGTCGGTTATCTTTCCGGTACCAATCTCTCTCTCTCTCTTACTTCTCTTCTTTTCTCCTATTCCGTTTCGTTGCTCGTCTGGAAATCTGTTCTCCTGTCTTTGCGTTTTAGGGATTAAGCCCGGAATTAGGGGTCCGTCAATGGTCACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATGTATGCTTATCAGAACTCGGCGGGTCGGATTATGGGATTCTTCCCCAATGAAGACGAGGTAGCTCGTTATAAGAAATAAGATTGCGCGTTTTCGTGTTCTTTCTTTTCCTTTTTTTGCTTTCTTCTTCTGAAATTTAAGTTCCATTTGTGGACACTGTTACGATGTTCACCTTATTAAACTTGCAATCAGGTTGTGTCAATAAGCAAATTTAATCTTCACTTTTTTCTGAAGA

mRNA sequence

GAGTCGCACAGCGAGGGAGAGAGCCGGCGGAGGTCAGTTTCAGCACCGGAGAACGTGATGAACACCGACATTACAGCTTCGACTAAGCCAGAGTATCCAGTAATAGATCGGAATCCTCCATTTACCAAAGTCGTTGGCAATTTCGACACTCTCGATTACCTCCGTTTCGTCACCATTACCGGCGTTTCCGTCACCGTCGGTTATCTTTCCGGGATTAAGCCCGGAATTAGGGGTCCGTCAATGGTCACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATGTATGCTTATCAGAACTCGGCGGGTCGGATTATGGGATTCTTCCCCAATGAAGACGAGGTAGCTCGTTATAAGAAATAAGATTGCGCGTTTTCGTGTTCTTTCTTTTCCTTTTTTTGCTTTCTTCTTCTGAAATTTAAGTTCCATTTGTGGACACTGTTACGATGTTCACCTTATTAAACTTGCAATCAGGTTGTGTCAATAAGCAAATTTAATCTTCACTTTTTTCTGAAGA

Coding sequence (CDS)

ATGAACACCGACATTACAGCTTCGACTAAGCCAGAGTATCCAGTAATAGATCGGAATCCTCCATTTACCAAAGTCGTTGGCAATTTCGACACTCTCGATTACCTCCGTTTCGTCACCATTACCGGCGTTTCCGTCACCGTCGGTTATCTTTCCGGGATTAAGCCCGGAATTAGGGGTCCGTCAATGGTCACCGGTGGTCTAATTGGGCTGATGGGAGGCTTCATGTATGCTTATCAGAACTCGGCGGGTCGGATTATGGGATTCTTCCCCAATGAAGACGAGGTAGCTCGTTATAAGAAATAA

Protein sequence

MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGPSMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK

Homology

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BLAST of Carg25113 vs. NCBI nr
Match: KAG7019380.1 (hypothetical protein SDJN02_18341 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 205.7 bits (522), Expect = 1.9e-49
Identity = 100/100 (100.00%), Postives = 100/100 (100.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 100

[Close]

BLAST of Carg25113 vs. NCBI nr
Match: XP_022927362.1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_023520383.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 203.0 bits (515), Expect = 1.2e-48
Identity = 99/100 (99.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. NCBI nr
Match: XP_022142552.1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Momordica charantia] >XP_022925305.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_022966760.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita maxima] >XP_023001508.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita maxima] >XP_023541604.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita pepo subsp. pepo] >KAG6573532.1 hypothetical protein SDJN03_27419, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012647.1 hypothetical protein SDJN02_25399 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 202.2 bits (513), Expect = 2.1e-48
Identity = 98/100 (98.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. NCBI nr
Match: XP_008463609.1 (PREDICTED: NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis melo] >XP_038894012.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Benincasa hispida])

HSP 1 Score: 201.1 bits (510), Expect = 4.6e-48
Identity = 97/100 (97.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPN+ EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVARYKK 100

[Close]

BLAST of Carg25113 vs. NCBI nr
Match: XP_011654950.1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis sativus] >XP_031745943.1 NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucumis sativus])

HSP 1 Score: 200.7 bits (509), Expect = 6.1e-48
Identity = 96/100 (96.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPV+DRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVVDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPN+ EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVARYKK 100

[Close]

BLAST of Carg25113 vs. ExPASy TrEMBL
Match: A0A6J1EGY7 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita moschata OX=3662 GN=LOC111434210 PE=4 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 5.9e-49
Identity = 99/100 (99.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. ExPASy TrEMBL
Match: A0A6J1HSH8 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita maxima OX=3661 GN=LOC111466374 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-48
Identity = 98/100 (98.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. ExPASy TrEMBL
Match: A0A6J1CN33 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111012637 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-48
Identity = 98/100 (98.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. ExPASy TrEMBL
Match: A0A6J1EHK1 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita moschata OX=3662 GN=LOC111432598 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.0e-48
Identity = 98/100 (98.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPNE EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNEGEVARYKK 100

[Close]

BLAST of Carg25113 vs. ExPASy TrEMBL
Match: A0A1S3CJL9 (NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103501718 PE=4 SV=1)

HSP 1 Score: 201.1 bits (510), Expect = 2.2e-48
Identity = 97/100 (97.00%), Postives = 99/100 (99.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP
Sbjct: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPN+ EVARYKK
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVARYKK 100

[Close]

BLAST of Carg25113 vs. TAIR 10
Match: AT4G16450.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: photorespiration; LOCATED IN: mitochondrion, mitochondrial membrane, mitochondrial respiratory chain complex I, respiratory chain complex I, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 178.7 bits (452), Expect = 2.3e-45
Identity = 85/100 (85.00%), Postives = 92/100 (92.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITA  K +YPV+DRNP FTKVVGNF TLDYLRF TITG+SVTVGYLSGIKPGI+GP
Sbjct: 1   MNTDITALEKAQYPVVDRNPAFTKVVGNFSTLDYLRFSTITGISVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPN+ EVA Y+K
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVASYQK 100

[Close]

BLAST of Carg25113 vs. TAIR 10
Match: AT4G16450.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: photorespiration; LOCATED IN: mitochondrion, mitochondrial membrane, mitochondrial respiratory chain complex I, respiratory chain complex I; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages. )

HSP 1 Score: 178.7 bits (452), Expect = 2.3e-45
Identity = 85/100 (85.00%), Postives = 92/100 (92.00%), Query Frame = 0


Query: 1   MNTDITASTKPEYPVIDRNPPFTKVVGNFDTLDYLRFVTITGVSVTVGYLSGIKPGIRGP 60
           MNTDITA  K +YPV+DRNP FTKVVGNF TLDYLRF TITG+SVTVGYLSGIKPGI+GP
Sbjct: 1   MNTDITALEKAQYPVVDRNPAFTKVVGNFSTLDYLRFSTITGISVTVGYLSGIKPGIKGP 60

Query: 61  SMVTGGLIGLMGGFMYAYQNSAGRIMGFFPNEDEVARYKK 101
           SMVTGGLIGLMGGFMYAYQNSAGR+MGFFPN+ EVA Y+K
Sbjct: 61  SMVTGGLIGLMGGFMYAYQNSAGRLMGFFPNDGEVASYQK 100

The following BLAST results are available for this feature:

BLAST of Carg25113 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019380.1	1.9e-49	100.00	hypothetical protein SDJN02_18341 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022927362.1	1.2e-48	99.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like [Cucurbita moschata] >XP_02...	[more]
XP_022142552.1	2.1e-48	98.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Momordica charantia] >XP_022925...	[more]
XP_008463609.1	4.6e-48	97.00	PREDICTED: NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis melo] >XP_03...	[more]
XP_011654950.1	6.1e-48	96.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit [Cucumis sativus] >XP_031745943....	[more]

BLAST of Carg25113 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg25113 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EGY7	5.9e-49	99.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1HSH8	1.0e-48	98.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1CN33	1.0e-48	98.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Momordica charantia OX=3673 G...	[more]
A0A6J1EHK1	1.0e-48	98.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit-like OS=Cucurbita moschata OX=36...	[more]
A0A1S3CJL9	2.2e-48	97.00	NADH-ubiquinone oxidoreductase 20.9 kDa subunit OS=Cucumis melo OX=3656 GN=LOC10...	[more]

BLAST of Carg25113 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16450.1	2.3e-45	85.00	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: photores...	[more]
AT4G16450.2	2.3e-45	85.00	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: photores...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR019721	NADH-ubiquinone oxidoreductase, 21kDa subunit, N-terminal	PFAM	PF10785	NADH-u_ox-rdase	coord: 11..89 e-value: 5.7E-20 score: 71.8
None	No IPR available	PANTHER	PTHR34062	OXIDOREDUCTASE 21 KDA SUBUNIT, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G04750)-RELATED	coord: 1..99
None	No IPR available	PANTHER	PTHR34062:SF2	NADH-UBIQUINONE OXIDOREDUCTASE 20.9 KDA SUBUNIT-LIKE	coord: 1..99

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25113-RA	Carg25113-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005747	mitochondrial respiratory chain complex I