Carg25064 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg25064
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	ethylene-responsive transcription factor 1-like
Location	Carg_Chr06: 6022437 .. 6025557 (-)
RNA-Seq Expression	Carg25064
Synteny	Carg25064

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCCTATGGTATTCCTATGCGCTTCGAGGCGACCGAAAGAGACCTTAATTTCCAAGGAAATTGTTACTCCGCTACATGTCGCACATCCGACCTCGACCGTATTATCTCCCATGTCTAGTTTTGTGTCACCGATCTCCACGTGATGTACCTAGGTCGCCCTAGCATATCAATTGTTATTATGTCGAACTCTCCGATGTTCATGCCGATAGTAATCGACAGTCTTGAGCGTCAGAAGTTATGCTAGCCGAGATCTTATTAGAGGTTTACTCGTGAAGGAGCCACAGCATCGACTTGGGGTCAAGAGGGGTGCAACTGAGATCAAGCAACATCCTTTCTTTGAAGGTGTGAATTGGGCTTTGATTCGATGCAGCACGCCCCCAGAAGTGCCTAGACCAATGGAGGCCGAACTGCCTAAGTTTGGGGTAGCTGACACAACAGGGGTTGGCGGCGGCGGCGGCGGCATCGGCGTCGGCGTCGGCAGCGGCAGTAAGAGGAGGGTAGGTACAGATGTTGTGAAGTCCGGTGGTCAATATCTTGACTTCGAGTTCTTTTAAATCTCGGATAAGCAGGATGGTTTGGTGGGGTCTAAAAGTAATTGGCCACAGGATGGTCTGTGTTGTTCGTTCTTCCAATATTGAATCCGCCTCATAGTTAGATAGATATTCCACTCCTAGAATTGGTACGTTTGATAAATTGAGTTTCTGAAAGAATCCACGTATTGCACGCTGTCTGATATGAACAAGTCTGAACCATGATGCTATTCTCATCAGATCTTCCCTTTGGTACACTGCAGTAAATTGCAATTCATGACCCATGTCTGCCTCTTTTTCATCAACAATCATCACTCACCACTGCTAATTACTGTCGTCTGTGCAAGGTAATCCCTTATAGATTGAGAATATGGTACCCGACCATAAATTGGCTCGACTTGATTCATTTGGTATTGAGAGTGAGTACCACACAATCGCAAAGTTCCTTAATTGTGAGCAAGACAGCTATACCTTTGCATAACAAAAACTAATTGCATTTGTTATAGTTCTAATGTTTATTATCAAGCCTCTGAACTGCTGTTGTTAGTCGCACACACCTATTCACTCGTCTAGAAGAATCGTGTTGTAAAAAAACCAACGCTTGTGCTCCGTAACCGATCAAAGATTCTGGACGATACACAACAATCAAATTCAGTCGTATCGATATCATAATTGGGCAAAACCATGGTCAGGTCGTCTCCTCTTACTCAATCAAGTTCAGTTCCACTAGCAACTCCAATTGATCAGAAAGAGAGGAAGAGGGTCGAGACAAGGATACAGAAAACAAGCCCTTTAGGCGATGCCCAATTCCTACAACTCTAGAGACAAAAGGGAGAGGACTCCGTTAAATAAACAATCAAATCATAATTCATTCAACTCAATACAATCCATAGCGAGAGATGCTATTGGGAGATACATAACCATTTTTCATCTCGATTCTCGCCCTCTTATTTTAATTTCATATTTATTTTCTTTTTTATTTTCATAAAGAGTGGAGTGGAGATTACTAATTAAGAAAAAAAAAATCAATTATATATATATATATATCTAATAATTTTTTAATGTCCATCTTTTACTACCATTTTTATTAGAAAAAAGTAAAATATTTTAATAAATTTAATAATATTTTATAAATATAAACTTATTTATTTTTTACTAAATTTAGACACGATTAACGACGGTAGTCCAGTTTAGCTAATGAATTTTCACTGTTCTTATAGTCAGTAATAACATCACTCTTCTCATCACTATTACAGATCTGTACATGAGATTTTCACCTCATACAGCTCCTTAAGGGCCATAAATAAATTTAAGGAGTGTGTCAGAATTATTTATCTTAATATGTATTCTTTAGAGAAGAGTACAGAATAAAAATCTATCGTGTGGCCCCCAATTAACCCAATAGAATTCTGTATATTCTAATAATAAAGAAAAAAAAGGTACTCTAAATTGATCTCATCCTTTAATAAAGGTATCTCTTCACTTGGTATATATTTTCTATATAAGCTGTAAATTTTTTATTATTCTGTATAGGTCAATTCAAAATCTATGTAAAAATTATTTTTATACCGACAAAATACTTTTTTGAAGACTTTTATGTTAGAAACAGATACGATATGGTGGTAGATATATGTGTGTGTATATATATATAAAAAGAAGAAATGAGAAATAGAAGTAACCATGAACGTTCAAGTGAGAACTGTCCCCATTACATATAAAATATTTGAAACACACGTTTTCAATGTTGTGGCCATGGTTTTGTGCACCTTTTTCATAGTGCTACCATTTTTTTAAAACCTTTTCTTATAGAATCTGACATAACCATAAATATTATATTAAAATTTTCAAATTAAAAAATACATTCAAACTTTCCCCAGTATTTAAGAATACCAATTTTGTTGGCATTTAAATTATACAAATTCTCACCGAATAATATAATAGAATATGAATTAGGAACACGGGGTAATTTGGAAGTTTCAAAGAAATAGACGTTGCGTTGAGCTGAATGATATATAAAGACATAGATATGAATAGTGAGTGTGGTTGAAATTAGTAGACTGAGAGAATCAAGGATGTCGAGTAACAGCAATTGCTCACAAGGGTCGTCGTCGTCATCGTCGTCAGAGGTGTTTCTGTTGGAGTGGATTCGGCGACATCTTTTGGAGGACGAGTTGGAATTCGGTTGGGGGATTAGGGAAAAATACTCCGAGTTTGACGAGTAGTGATGGGGTTACGGATATCGAGGCCCCCGGGAAGGGGAGTTCGATTAGAGGGCTGCAGTTCAGAGGAGTGAGGAGGAGGCCATGGGGGAAGTACGCAGCAGAGATCAGAGACCCCAATAGAAAAGGGGCTCGCATATGGCTGGGGACATACCAGACCGCTGAAGAGGCTGCTTTGGCTTACGATCGAGCTGCTTTTCAGATGCGGGGAGCCAAGGCCAAGCTCAACTTTCCTCACATGATTGGTTCCGCGGAGGCGCTGATTGGCATAGGCTGCTGTTACCCTAAGCGCGCTTCCCCTGATTCCCCTCCGCCTCTGTTCAGCGTCAAGCGCCCTAGAACCGACTCGCCCTCAATGCCACGATGTTAA

mRNA sequence

ATGTCCTATGTCTTGAGCGTCAGAAGTTATGCTAGCCGAGATCTTATTAGAGGTTTACTCGTGAAGGAGCCACAGCATCGACTTGGGGTCAAGAGGGGTGCAACTGAGATCAAGCAACATCCTTTCTTTGAAGGTGTGAATTGGGCTTTGATTCGATGCAGCACGCCCCCAGAAGTGCCTAGACCAATGGAGGCCGAACTGCCTAAGTTTGGGGTAGCTGACACAACAGGGGTTGGCGGCGGCGGCGGCGGCATCGGCGTCGGCGTCGGCAGCGGCACAATTGCTCACAAGGGTCGTCGTCGTCATCGTCGTCAGAGGTGTTTCTGTTGGAGTGGATTCGGCGACATCTTTTGGAGGACGAGTTGGAATTCGGTTGGGGGATTAGGGAAAAATACTCCGAGTTTGACGAGTAGTGATGGGGTTACGGATATCGAGGCCCCCGGGAAGGGGAGTTCGATTAGAGGGCTGCAGTTCAGAGGAGTGAGGAGGAGGCCATGGGGGAAGTACGCAGCAGAGATCAGAGACCCCAATAGAAAAGGGGCTCGCATATGGCTGGGGACATACCAGACCGCTGAAGAGGCTGCTTTGGCTTACGATCGAGCTGCTTTTCAGATGCGGGGAGCCAAGGCCAAGCTCAACTTTCCTCACATGATTGGTTCCGCGGAGGCGCTGATTGGCATAGGCTGCTGTTACCCTAAGCGCGCTTCCCCTGATTCCCCTCCGCCTCTGTTCAGCGTCAAGCGCCCTAGAACCGACTCGCCCTCAATGCCACGATGTTAA

Coding sequence (CDS)

ATGTCCTATGTCTTGAGCGTCAGAAGTTATGCTAGCCGAGATCTTATTAGAGGTTTACTCGTGAAGGAGCCACAGCATCGACTTGGGGTCAAGAGGGGTGCAACTGAGATCAAGCAACATCCTTTCTTTGAAGGTGTGAATTGGGCTTTGATTCGATGCAGCACGCCCCCAGAAGTGCCTAGACCAATGGAGGCCGAACTGCCTAAGTTTGGGGTAGCTGACACAACAGGGGTTGGCGGCGGCGGCGGCGGCATCGGCGTCGGCGTCGGCAGCGGCACAATTGCTCACAAGGGTCGTCGTCGTCATCGTCGTCAGAGGTGTTTCTGTTGGAGTGGATTCGGCGACATCTTTTGGAGGACGAGTTGGAATTCGGTTGGGGGATTAGGGAAAAATACTCCGAGTTTGACGAGTAGTGATGGGGTTACGGATATCGAGGCCCCCGGGAAGGGGAGTTCGATTAGAGGGCTGCAGTTCAGAGGAGTGAGGAGGAGGCCATGGGGGAAGTACGCAGCAGAGATCAGAGACCCCAATAGAAAAGGGGCTCGCATATGGCTGGGGACATACCAGACCGCTGAAGAGGCTGCTTTGGCTTACGATCGAGCTGCTTTTCAGATGCGGGGAGCCAAGGCCAAGCTCAACTTTCCTCACATGATTGGTTCCGCGGAGGCGCTGATTGGCATAGGCTGCTGTTACCCTAAGCGCGCTTCCCCTGATTCCCCTCCGCCTCTGTTCAGCGTCAAGCGCCCTAGAACCGACTCGCCCTCAATGCCACGATGTTAA

Protein sequence

MSYVLSVRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELPKFGVADTTGVGGGGGGIGVGVGSGTIAHKGRRRHRRQRCFCWSGFGDIFWRTSWNSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLFSVKRPRTDSPSMPRC

Homology

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BLAST of Carg25064 vs. NCBI nr
Match: KAG7028473.1 (Ethylene-responsive transcription factor 13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 535.0 bits (1377), Expect = 3.5e-148
Identity = 259/259 (100.00%), Postives = 259/259 (100.00%), Query Frame = 0


Query: 1   MSYVLSVRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVP 60
           MSYVLSVRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVP
Sbjct: 1   MSYVLSVRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVP 60

Query: 61  RPMEAELPKFGVADTTGVGGGGGGIGVGVGSGTIAHKGRRRHRRQRCFCWSGFGDIFWRT 120
           RPMEAELPKFGVADTTGVGGGGGGIGVGVGSGTIAHKGRRRHRRQRCFCWSGFGDIFWRT
Sbjct: 61  RPMEAELPKFGVADTTGVGGGGGGIGVGVGSGTIAHKGRRRHRRQRCFCWSGFGDIFWRT 120

Query: 121 SWNSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKG 180
           SWNSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKG
Sbjct: 121 SWNSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKG 180

Query: 181 ARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSP 240
           ARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSP
Sbjct: 181 ARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSP 240

Query: 241 PPLFSVKRPRTDSPSMPRC 260
           PPLFSVKRPRTDSPSMPRC
Sbjct: 241 PPLFSVKRPRTDSPSMPRC 259

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BLAST of Carg25064 vs. NCBI nr
Match: KAG6597005.1 (Ethylene-responsive transcription factor 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 279.6 bits (714), Expect = 2.7e-71
Identity = 136/137 (99.27%), Postives = 137/137 (100.00%), Query Frame = 0


Query: 123 NSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 182
           +SVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR
Sbjct: 35  DSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 94

Query: 183 IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP 242
           IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP
Sbjct: 95  IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP 154

Query: 243 LFSVKRPRTDSPSMPRC 260
           LFSVKRPRTDSPSMPRC
Sbjct: 155 LFSVKRPRTDSPSMPRC 171

[Close]

BLAST of Carg25064 vs. NCBI nr
Match: XP_022950239.1 (ethylene-responsive transcription factor 1-like [Cucurbita moschata])

HSP 1 Score: 275.8 bits (704), Expect = 3.8e-70
Identity = 134/135 (99.26%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 125 VGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW 184
           VGGLGKNTPSL SSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW
Sbjct: 34  VGGLGKNTPSLASSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW 93

Query: 185 LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF 244
           LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF
Sbjct: 94  LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF 153

Query: 245 SVKRPRTDSPSMPRC 260
           SVKRPRTDSPSMPRC
Sbjct: 154 SVKRPRTDSPSMPRC 168

[Close]

BLAST of Carg25064 vs. NCBI nr
Match: XP_022974259.1 (ethylene-responsive transcription factor 13-like [Cucurbita maxima])

HSP 1 Score: 273.9 bits (699), Expect = 1.5e-69
Identity = 133/137 (97.08%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 123 NSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 182
           +SVGGLGKNTPSL S DGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR
Sbjct: 32  DSVGGLGKNTPSLKSGDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 91

Query: 183 IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP 242
           IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASP+SPPP
Sbjct: 92  IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPNSPPP 151

Query: 243 LFSVKRPRTDSPSMPRC 260
           LFSVKRPRTDSPSMPRC
Sbjct: 152 LFSVKRPRTDSPSMPRC 168

[Close]

BLAST of Carg25064 vs. NCBI nr
Match: XP_023539438.1 (ethylene-responsive transcription factor 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 270.8 bits (691), Expect = 1.2e-68
Identity = 131/137 (95.62%), Postives = 134/137 (97.81%), Query Frame = 0


Query: 123 NSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 182
           +SVGGLGKNTPSLTS DGVTD E PGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR
Sbjct: 31  DSVGGLGKNTPSLTSIDGVTDTETPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 90

Query: 183 IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP 242
           IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCC+PKRASPDSPPP
Sbjct: 91  IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCHPKRASPDSPPP 150

Query: 243 LFSVKRPRTDSPSMPRC 260
           LFSVKRPRTDSP+MPRC
Sbjct: 151 LFSVKRPRTDSPAMPRC 167

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BLAST of Carg25064 vs. ExPASy Swiss-Prot
Match: Q8L9K1 (Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=ERF13 PE=1 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 4.2e-27
Identity = 64/115 (55.65%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 130 KNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQ 189
           +N P  T + G     AP +    +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY+
Sbjct: 71  ENKPPATKASG---SHAPRQ----KGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYE 130

Query: 190 TAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCY------PKRASPD 239
           T E+AA+AYDRAAFQ+RG+KAKLNFPH+IGS        C Y      P+R SP+
Sbjct: 131 TPEDAAVAYDRAAFQLRGSKAKLNFPHLIGS--------CKYEPVRIRPRRRSPE 170

[Close]

BLAST of Carg25064 vs. ExPASy Swiss-Prot
Match: Q40476 (Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum OX=4097 GN=ERF1 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-24
Identity = 54/85 (63.53%), Postives = 67/85 (78.82%), Query Frame = 0


Query: 154 RGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLN 213
           RG  +RGVRRRPWGK+AAEIRDP + GAR+WLGTY+T EEAA+AYD+AA++MRG+KA LN
Sbjct: 101 RGRHYRGVRRRPWGKFAAEIRDPAKNGARVWLGTYETDEEAAIAYDKAAYRMRGSKAHLN 160

Query: 214 FPHMIGSAEALIGIGCCYPKRASPD 239
           FPH IG  E    +     +RASP+
Sbjct: 161 FPHRIGLNEP-EPVRVTAKRRASPE 184

[Close]

BLAST of Carg25064 vs. ExPASy Swiss-Prot
Match: A2YBX5 (Protein kinase G11A OS=Oryza sativa subsp. indica OX=39946 GN=OsI_021818 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-24
Identity = 51/62 (82.26%), Postives = 57/62 (91.94%), Query Frame = 0


Query: 7   VRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAE 66
           V S+++RDLIRGLLVKEPQ RLG KRGATEIKQHPFFEGVNWALIRC++PPEVPRP+E E
Sbjct: 497 VVSFSARDLIRGLLVKEPQQRLGCKRGATEIKQHPFFEGVNWALIRCASPPEVPRPVEIE 556

Query: 67  LP 69
            P
Sbjct: 557 RP 558

[Close]

BLAST of Carg25064 vs. ExPASy Swiss-Prot
Match: Q0DCT8 (Protein kinase G11A OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0291600 PE=2 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-24
Identity = 51/62 (82.26%), Postives = 57/62 (91.94%), Query Frame = 0


Query: 7   VRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAE 66
           V S+++RDLIRGLLVKEPQ RLG KRGATEIKQHPFFEGVNWALIRC++PPEVPRP+E E
Sbjct: 497 VVSFSARDLIRGLLVKEPQQRLGCKRGATEIKQHPFFEGVNWALIRCASPPEVPRPVEIE 556

Query: 67  LP 69
            P
Sbjct: 557 RP 558

[Close]

BLAST of Carg25064 vs. ExPASy Swiss-Prot
Match: Q9LW50 (Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris OX=4096 GN=ERF2 PE=2 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 5.6e-24
Identity = 50/69 (72.46%), Postives = 61/69 (88.41%), Query Frame = 0


Query: 154 RGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQTAEEAALAYDRAAFQMRGAKAKLN 213
           +G  +RGVR+RPWGK+AAEIRDP + GAR+WLGTY+TAEEAALAYD+AA++MRG+KA LN
Sbjct: 99  KGRHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTYETAEEAALAYDKAAYRMRGSKALLN 158

Query: 214 FPHMIGSAE 223
           FPH IG  E
Sbjct: 159 FPHRIGLNE 167

[Close]

BLAST of Carg25064 vs. ExPASy TrEMBL
Match: A0A6J1GF81 (ethylene-responsive transcription factor 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453384 PE=4 SV=1)

HSP 1 Score: 275.8 bits (704), Expect = 1.9e-70
Identity = 134/135 (99.26%), Postives = 134/135 (99.26%), Query Frame = 0


Query: 125 VGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW 184
           VGGLGKNTPSL SSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW
Sbjct: 34  VGGLGKNTPSLASSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIW 93

Query: 185 LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF 244
           LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF
Sbjct: 94  LGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPPLF 153

Query: 245 SVKRPRTDSPSMPRC 260
           SVKRPRTDSPSMPRC
Sbjct: 154 SVKRPRTDSPSMPRC 168

[Close]

BLAST of Carg25064 vs. ExPASy TrEMBL
Match: A0A6J1IH30 (ethylene-responsive transcription factor 13-like OS=Cucurbita maxima OX=3661 GN=LOC111472899 PE=4 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 7.1e-70
Identity = 133/137 (97.08%), Postives = 135/137 (98.54%), Query Frame = 0


Query: 123 NSVGGLGKNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 182
           +SVGGLGKNTPSL S DGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR
Sbjct: 32  DSVGGLGKNTPSLKSGDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGAR 91

Query: 183 IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPDSPPP 242
           IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASP+SPPP
Sbjct: 92  IWLGTYQTAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCYPKRASPNSPPP 151

Query: 243 LFSVKRPRTDSPSMPRC 260
           LFSVKRPRTDSPSMPRC
Sbjct: 152 LFSVKRPRTDSPSMPRC 168

[Close]

BLAST of Carg25064 vs. ExPASy TrEMBL
Match: A0A6J1F5B6 (serine/threonine-protein kinase D6PKL1-like OS=Cucurbita moschata OX=3662 GN=LOC111442445 PE=4 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.0e-36
Identity = 81/85 (95.29%), Postives = 82/85 (96.47%), Query Frame = 0


Query: 9   SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 68
           SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP
Sbjct: 632 SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 691

Query: 69  KFGVADTTGVGGGGGGIGVGVGSGT 94
           KFGVADTTGVGGGG   GVGVGSG+
Sbjct: 692 KFGVADTTGVGGGG---GVGVGSGS 713

[Close]

BLAST of Carg25064 vs. ExPASy TrEMBL
Match: A0A6J1IH41 (serine/threonine-protein kinase D6PKL1-like OS=Cucurbita maxima OX=3661 GN=LOC111472908 PE=4 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 1.8e-36
Identity = 80/83 (96.39%), Postives = 80/83 (96.39%), Query Frame = 0


Query: 9   SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 68
           SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP
Sbjct: 632 SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 691

Query: 69  KFGVADTTGVGGGGGGIGVGVGS 92
           KFGVADTTGVGGGGG  GVG GS
Sbjct: 692 KFGVADTTGVGGGGGVGGVGGGS 714

[Close]

BLAST of Carg25064 vs. ExPASy TrEMBL
Match: A0A6J1D6F7 (serine/threonine-protein kinase D6PKL1-like OS=Momordica charantia OX=3673 GN=LOC111017488 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 1.2e-32
Identity = 70/71 (98.59%), Postives = 70/71 (98.59%), Query Frame = 0


Query: 9   SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 68
           SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP
Sbjct: 633 SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAELP 692

Query: 69  KFGVADTTGVG 80
           KFGV DTTGVG
Sbjct: 693 KFGVVDTTGVG 703

[Close]

BLAST of Carg25064 vs. TAIR 10
Match: AT2G44840.1 (ethylene-responsive element binding factor 13 )

HSP 1 Score: 123.2 bits (308), Expect = 3.0e-28
Identity = 64/115 (55.65%), Postives = 81/115 (70.43%), Query Frame = 0


Query: 130 KNTPSLTSSDGVTDIEAPGKGSSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQ 189
           +N P  T + G     AP +    +G+Q+RGVRRRPWGK+AAEIRDP + GAR+WLGTY+
Sbjct: 71  ENKPPATKASG---SHAPRQ----KGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYE 130

Query: 190 TAEEAALAYDRAAFQMRGAKAKLNFPHMIGSAEALIGIGCCY------PKRASPD 239
           T E+AA+AYDRAAFQ+RG+KAKLNFPH+IGS        C Y      P+R SP+
Sbjct: 131 TPEDAAVAYDRAAFQLRGSKAKLNFPHLIGS--------CKYEPVRIRPRRRSPE 170

[Close]

BLAST of Carg25064 vs. TAIR 10
Match: AT2G44830.1 (Protein kinase superfamily protein )

HSP 1 Score: 115.9 bits (289), Expect = 4.7e-26
Identity = 55/72 (76.39%), Postives = 60/72 (83.33%), Query Frame = 0


Query: 9   SYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAE-L 68
           SYA RDLI+ LLVK+P++RLG KRGATEIKQHPFFEGVNWALIRCSTPPEVPR ME E  
Sbjct: 663 SYAGRDLIQALLVKDPKNRLGTKRGATEIKQHPFFEGVNWALIRCSTPPEVPRQMETEPP 722

Query: 69  PKFGVADTTGVG 80
           PK+G  D  G G
Sbjct: 723 PKYGPIDPVGFG 734

[Close]

BLAST of Carg25064 vs. TAIR 10
Match: AT5G55910.1 (D6 protein kinase )

HSP 1 Score: 108.2 bits (269), Expect = 9.9e-24
Identity = 46/60 (76.67%), Postives = 55/60 (91.67%), Query Frame = 0


Query: 7   VRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAE 66
           V S+A+RDLIR LLVKEPQHRL  KRGATEIKQHPFFEGVNWAL+RC++PPE+P+P++ E
Sbjct: 410 VVSFAARDLIRSLLVKEPQHRLAYKRGATEIKQHPFFEGVNWALVRCASPPEIPKPVDLE 469

[Close]

BLAST of Carg25064 vs. TAIR 10
Match: AT4G17500.1 (ethylene responsive element binding factor 1 )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 47/72 (65.28%), Postives = 62/72 (86.11%), Query Frame = 0


Query: 151 SSIRGLQFRGVRRRPWGKYAAEIRDPNRKGARIWLGTYQTAEEAALAYDRAAFQMRGAKA 210
           ++ +G  +RGVR+RPWGK+AAEIRDP + GAR+WLGT++TAE+AALAYDRAAF+MRG++A
Sbjct: 141 TAAKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRA 200

Query: 211 KLNFPHMIGSAE 223
            LNFP  + S E
Sbjct: 201 LLNFPLRVNSGE 212

[Close]

BLAST of Carg25064 vs. TAIR 10
Match: AT5G47750.1 (D6 protein kinase like 2 )

HSP 1 Score: 107.8 bits (268), Expect = 1.3e-23
Identity = 52/87 (59.77%), Postives = 64/87 (73.56%), Query Frame = 0


Query: 7   VRSYASRDLIRGLLVKEPQHRLGVKRGATEIKQHPFFEGVNWALIRCSTPPEVPRPMEAE 66
           V S+A+RDLIRGLL+KEPQ RLG KRGATE+KQHPFFEGVNWALIRC+TPPE+P+P+E E
Sbjct: 491 VVSFAARDLIRGLLMKEPQQRLGFKRGATEVKQHPFFEGVNWALIRCATPPEIPKPVELE 550

Query: 67  LPKFGVADTTGVGGGGGGIGVGVGSGT 94
                VA+         G+ +    G+
Sbjct: 551 KGAVSVAEAPSSQKTAAGLVLNAQKGS 577

The following BLAST results are available for this feature:

BLAST of Carg25064 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7028473.1	3.5e-148	100.00	Ethylene-responsive transcription factor 13, partial [Cucurbita argyrosperma sub...	[more]
KAG6597005.1	2.7e-71	99.27	Ethylene-responsive transcription factor 13, partial [Cucurbita argyrosperma sub...	[more]
XP_022950239.1	3.8e-70	99.26	ethylene-responsive transcription factor 1-like [Cucurbita moschata]	[more]
XP_022974259.1	1.5e-69	97.08	ethylene-responsive transcription factor 13-like [Cucurbita maxima]	[more]
XP_023539438.1	1.2e-68	95.62	ethylene-responsive transcription factor 13-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg25064 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9K1	4.2e-27	55.65	Ethylene-responsive transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=E...	[more]
Q40476	3.3e-24	63.53	Ethylene-responsive transcription factor 1 OS=Nicotiana tabacum OX=4097 GN=ERF1 ...	[more]
A2YBX5	3.3e-24	82.26	Protein kinase G11A OS=Oryza sativa subsp. indica OX=39946 GN=OsI_021818 PE=2 SV...	[more]
Q0DCT8	3.3e-24	82.26	Protein kinase G11A OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0291600 PE=...	[more]
Q9LW50	5.6e-24	72.46	Ethylene-responsive transcription factor 2 OS=Nicotiana sylvestris OX=4096 GN=ER...	[more]

BLAST of Carg25064 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GF81	1.9e-70	99.26	ethylene-responsive transcription factor 1-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1IH30	7.1e-70	97.08	ethylene-responsive transcription factor 13-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1F5B6	1.0e-36	95.29	serine/threonine-protein kinase D6PKL1-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IH41	1.8e-36	96.39	serine/threonine-protein kinase D6PKL1-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A6J1D6F7	1.2e-32	98.59	serine/threonine-protein kinase D6PKL1-like OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Carg25064 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G44840.1	3.0e-28	55.65	ethylene-responsive element binding factor 13	[more]
AT2G44830.1	4.7e-26	76.39	Protein kinase superfamily protein	[more]
AT5G55910.1	9.9e-24	76.67	D6 protein kinase	[more]
AT4G17500.1	1.3e-23	65.28	ethylene responsive element binding factor 1	[more]
AT5G47750.1	1.3e-23	59.77	D6 protein kinase like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001471	AP2/ERF domain	PRINTS	PR00367	ETHRSPELEMNT	coord: 158..169 score: 65.91 coord: 181..197 score: 65.99
IPR001471	AP2/ERF domain	SMART	SM00380	rav1_2	coord: 157..221 e-value: 5.7E-35 score: 132.2
IPR001471	AP2/ERF domain	PFAM	PF00847	AP2	coord: 158..207 e-value: 4.0E-15 score: 55.8
IPR001471	AP2/ERF domain	PROSITE	PS51032	AP2_ERF	coord: 157..215 score: 23.828024
IPR001471	AP2/ERF domain	CDD	cd00018	AP2	coord: 157..216 e-value: 8.46996E-30 score: 104.661
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 10..60 e-value: 9.7E-9 score: 36.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 237..259
None	No IPR available	PANTHER	PTHR31190:SF173	PATHOGENESIS-RELATED GENES TRANSCRIPTIONAL ACTIVATOR PTI5	coord: 153..220
IPR036955	AP2/ERF domain superfamily	GENE3D	3.30.730.10	AP2/ERF domain	coord: 156..217 e-value: 1.0E-28 score: 101.3
IPR044808	Ethylene-responsive transcription factor	PANTHER	PTHR31190	DNA-BINDING DOMAIN	coord: 153..220
IPR016177	DNA-binding domain superfamily	SUPERFAMILY	54171	DNA-binding domain	coord: 158..216
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 9..66

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg25064-RA	Carg25064-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009873	ethylene-activated signaling pathway
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0006355	regulation of transcription, DNA-templated
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0004674	protein serine/threonine kinase activity