Carg25013 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg25013
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionSpoU_methylase domain-containing protein
LocationCarg_Chr11: 6416496 .. 6417340 (-)
RNA-Seq ExpressionCarg25013
SyntenyCarg25013
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTGCATTTGTGGATGAGGAGGAGGGTGTCTATCTTTTCTTCTACCGCTGTCTGAACCGTCGAAGCTCTTCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTGTACTCTTCTCTCACTCCCTCTCTTTCTCGCTCTCGGAGGCCATACAATATCTTTTGCATTTCTTTCTTAAAGCTGTTCTTGACTTTCCTTCTGTTTTGATTGCTTTACAGCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGA

mRNA sequence

CACTGCATTTGTGGATGAGGAGGAGGGTGTCTATCTTTTCTTCTACCGCTGTCTGAACCGTCGAAGCTCTTCAACGCGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGA

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTATTTCTACTTGGAAGCTGCAGTCCCCGTACAACTCAACGAATTCTCCCTTCTGTTTCCTTCTCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCCTCAAGAAACAACCCTAAGGCAGTTTCTACAAGATGTTTTTCCTCGACTTCGGCCGCTGCATCAGTAGAAGTAACAGACTCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGATGAATATCCGCGTCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAGTTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGCGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCGGTTTGTTTGGTGGTAGAAGGTTTGAGTGATTTCGGTAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGA

Protein sequence

MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVSTRCFSSTSAAASVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGDEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND
Homology
BLAST of Carg25013 vs. NCBI nr
Match: KAG7022329.1 (hypothetical protein SDJN02_16060 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 430.6 bits (1106), Expect = 7.8e-117
Identity = 216/216 (100.00%), Postives = 216/216 (100.00%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAASVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGD 120
           TRCFSSTSAAASVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGD
Sbjct: 61  TRCFSSTSAAASVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGD 120

Query: 121 EYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEG 180
           EYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEG
Sbjct: 121 EYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND
Sbjct: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 216

BLAST of Carg25013 vs. NCBI nr
Match: XP_022933839.1 (uncharacterized protein LOC111441136 [Cucurbita moschata])

HSP 1 Score: 414.1 bits (1063), Expect = 7.5e-112
Identity = 211/217 (97.24%), Postives = 213/217 (98.16%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
            RCFSSTSAAA SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. NCBI nr
Match: KAG6588498.1 (hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 412.9 bits (1060), Expect = 1.7e-111
Identity = 211/217 (97.24%), Postives = 213/217 (98.16%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCFSSTSAAA SVEVTDSVGSDLEPPEIHTQDTVEHLLTHS DVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSYDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. NCBI nr
Match: XP_023530258.1 (uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 408.7 bits (1049), Expect = 3.2e-110
Identity = 209/217 (96.31%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCFSSTSAAA SVEVTDSVGSDLEPPEIH QDTVE+LLTHSDDVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDSVGSDLEPPEIHAQDTVENLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. NCBI nr
Match: XP_022969711.1 (uncharacterized protein LOC111468788 [Cucurbita maxima])

HSP 1 Score: 399.1 bits (1024), Expect = 2.5e-107
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQ ILPSVSF SPPLSSYSPFFTAVSNHSSRNNPKAV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCFSSTSAAA SVEVTD+VGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP QGRWFPYLDKFSCE+SWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. ExPASy TrEMBL
Match: A0A6J1F0V5 (uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC111441136 PE=3 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 3.6e-112
Identity = 211/217 (97.24%), Postives = 213/217 (98.16%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS
Sbjct: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
            RCFSSTSAAA SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG
Sbjct: 61  IRCFSSTSAAASSVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
           DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. ExPASy TrEMBL
Match: A0A6J1HYK4 (uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788 PE=3 SV=1)

HSP 1 Score: 399.1 bits (1024), Expect = 1.2e-107
Identity = 204/217 (94.01%), Postives = 208/217 (95.85%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRIAVRPSLFLLGSCS RTTQ ILPSVSF SPPLSSYSPFFTAVSNHSSRNNPKAV 
Sbjct: 1   MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVF 60

Query: 61  TRCFSSTSAAA-SVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           TRCFSSTSAAA SVEVTD+VGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG
Sbjct: 61  TRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP QGRWFPYLDKFSCE+SWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE
Sbjct: 121 AEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217

BLAST of Carg25013 vs. ExPASy TrEMBL
Match: A0A0A0LC15 (SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824750 PE=3 SV=1)

HSP 1 Score: 309.3 bits (791), Expect = 1.3e-80
Identity = 161/216 (74.54%), Postives = 179/216 (82.87%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SLFLL SCSPR TQRI  SV   S  L S+S FFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLFLLQSCSPRRTQRIFSSVFLPSLHLPSFSLFFT---NRFSRSNPNAVS 60

Query: 61  TRCFSSTSAAASVEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGD 120
           TRCF+S SAA S    + + +D+EPPE+ TQDTVEHLLTHS+DVARLMKMERR + LDG 
Sbjct: 61  TRCFASISAATSPLEVEDIVADVEPPEVRTQDTVEHLLTHSNDVARLMKMERRSVELDGA 120

Query: 121 EYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEG 180
           EYP +GRWFPYLDK+SC SSWLNSGE+L+ALD LILDSRKERFKNVVR+RSYSVCLVVEG
Sbjct: 121 EYPHKGRWFPYLDKYSCGSSWLNSGEILEALDSLILDSRKERFKNVVRNRSYSVCLVVEG 180

Query: 181 LSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           L+DFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 LTDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 213

BLAST of Carg25013 vs. ExPASy TrEMBL
Match: A0A5A7UJ80 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G007650 PE=3 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 3.8e-77
Identity = 162/217 (74.65%), Postives = 181/217 (83.41%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SL LL S SPR TQR+ PSV   S  L S SPFFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60

Query: 61  TRCFSSTSAAAS-VEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +RCF+S SAA S +E+ D V +D+EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP+ LDG
Sbjct: 61  SRCFASISAATSPLELADIV-ADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDK+S  SSWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 212

BLAST of Carg25013 vs. ExPASy TrEMBL
Match: A0A1S3B8M3 (tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486969 PE=3 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 3.8e-77
Identity = 162/217 (74.65%), Postives = 181/217 (83.41%), Query Frame = 0

Query: 1   MASCRIAVRPSLFLLGSCSPRTTQRILPSVSFSSPPLSSYSPFFTAVSNHSSRNNPKAVS 60
           MASCRI  R SL LL S SPR TQR+ PSV   S  L S SPFFT   N  SR+NP AVS
Sbjct: 1   MASCRIVSRSSLTLLQSFSPRRTQRVFPSVFLPSLHLPS-SPFFT---NTLSRSNPNAVS 60

Query: 61  TRCFSSTSAAAS-VEVTDSVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDG 120
           +RCF+S SAA S +E+ D V +D+EPPE+ TQDTVEHLLTHSDDV+RLMKMERRP+ LDG
Sbjct: 61  SRCFASISAATSPLELADIV-ADVEPPEVPTQDTVEHLLTHSDDVSRLMKMERRPVELDG 120

Query: 121 DEYPRQGRWFPYLDKFSCESSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVE 180
            EYP +GRWFPYLDK+S  SSWLNSGE+L+ALD LILD+RKERFKNVVR+RSYSVCLVVE
Sbjct: 121 AEYPHKGRWFPYLDKYSFGSSWLNSGEILEALDSLILDTRKERFKNVVRNRSYSVCLVVE 180

Query: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRCKND 217
           GLSDFGNVSAAFRSADALGFQSVHVVSCDASKR + +
Sbjct: 181 GLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 212

BLAST of Carg25013 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 145.2 bits (365), Expect = 6.1e-35
Identity = 75/123 (60.98%), Postives = 94/123 (76.42%), Query Frame = 0

Query: 91  QDTVEHLL-THSDDVARLMKMERRPINLDGDEYPRQGRWFPYLDKFSCESSWLNSGEVLD 150
           +DTV+ LL T+  + + LMKMERR  +L   E   +G WFPY D+F C    L+S EVL+
Sbjct: 72  KDTVKGLLTTNRGEASSLMKMERR-CSLSNGEGDCRGSWFPYEDRFRCGEVHLSSREVLE 131

Query: 151 ALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDA 210
           A+ P +++ R +RF+ VV +RSYSVCLVVEGLSDFGN+SAAFRSADALG QSVHVVSCD+
Sbjct: 132 AVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAFRSADALGIQSVHVVSCDS 191

Query: 211 SKR 213
           SKR
Sbjct: 192 SKR 193

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7022329.17.8e-117100.00hypothetical protein SDJN02_16060 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022933839.17.5e-11297.24uncharacterized protein LOC111441136 [Cucurbita moschata][more]
KAG6588498.11.7e-11197.24hypothetical protein SDJN03_17063, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023530258.13.2e-11096.31uncharacterized protein LOC111792877 [Cucurbita pepo subsp. pepo][more]
XP_022969711.12.5e-10794.01uncharacterized protein LOC111468788 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1F0V53.6e-11297.24uncharacterized protein LOC111441136 OS=Cucurbita moschata OX=3662 GN=LOC1114411... [more]
A0A6J1HYK41.2e-10794.01uncharacterized protein LOC111468788 OS=Cucurbita maxima OX=3661 GN=LOC111468788... [more]
A0A0A0LC151.3e-8074.54SpoU_methylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G824... [more]
A0A5A7UJ803.8e-7774.65tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3B8M33.8e-7774.65tRNA (Guanosine(18)-2'-O)-methyltransferase OS=Cucumis melo OX=3656 GN=LOC103486... [more]
Match NameE-valueIdentityDescription
AT5G15390.16.1e-3560.98tRNA/rRNA methyltransferase (SpoU) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029026tRNA (guanine-N1-)-methyltransferase, N-terminalGENE3D3.40.1280.10coord: 150..216
e-value: 1.4E-12
score: 49.2
NoneNo IPR availablePANTHERPTHR43453:SF1RRNA METHYLASE-LIKE PROTEINcoord: 1..215
IPR033671tRNA (guanosine(18)-2'-O)-methyltransferasePANTHERPTHR43453RRNA METHYLASE-LIKEcoord: 1..215
IPR029028Alpha/beta knot methyltransferasesSUPERFAMILY75217alpha/beta knotcoord: 157..208

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg25013-RACarg25013-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030488 tRNA methylation
molecular_function GO:0008173 RNA methyltransferase activity
molecular_function GO:0000049 tRNA binding
molecular_function GO:0008168 methyltransferase activity