Carg24976 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg24976
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionQueuine tRNA-ribosyltransferase accessory subunit 2
LocationCarg_Chr15: 165118 .. 169576 (+)
RNA-Seq ExpressionCarg24976
SyntenyCarg24976
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATTCGCGGTGAAGGCATGGGGCAACGGCAAGGCGCGCACCGGATTTCTACAGCTCGTCCACTGCGCTTCCGCCATGGAGACTCCCGCGTTACTTCTCTCTACTCGCAAAGGACTGCCTAATTTCAGCTCTCCTGATCTTCTTCCCTCTCTCCCTTCGCCTGATTCTCACCTCCTCCAATTCAGTCCTCTTCACTTGTAAGTATTCTACACAATCCCTACGCCTTCAGCTGCATTCAATCGTTTTCATTTTAATTCGTTTGCGTTTCGATGTACTGATCACTCACTTCATTCTGCGTTCACTGTGTTTCTTAATTGGAGTGTCTCGCTCTACTTTGTTGCATGATTTTTCTTTCATTTATTTCGTCCCGTCTAGCTTAAAGATTGTTTGTCTGCGAGGACGATTCCTGGACTAGAAACTATTCTACAATCATGAAAATAGACTGGCTAAAAGCATGAGATAAGAAGTTCGTACTTGGAGAATAATCGTGCGGTACCTTTTGGATTTTTCTAGGGGAATATGTCATATACGGAAAAGGACGAAATTACTGGCGAATGGATGTAGAGCGTGTAATCATGGGAGTTGGATGATAATTAATGTCCGCTGTGATTCTTAACTATCTGAACGGCTGAGTTAATGATGCAACGACCTAATTTATTGATGTTTCGATTTATGCTTCTAGGCTCTTCTTACACAGCTTATCTATGGATTTTTTTTTTATTATTAATCATTTGTATGCGTGAAAGAACAGTCAAGGAAATTTGTGGAATCACATTGCTTTATCTATCCATTTTGGTCCTCGATTCAACTTCCGTAATTAAATAAATTTTTAACTTTTAGATGACATGCTGTAATGAAATTTGTTTCTCTCATTTTTAGATTTTATCAGATGAGTTCATTTTTGTACAGGTAGTAGGTACATTAATTGATTCTCTACGTTGTTCTCGATGTTGGTGGCTGTTTTTCTTTAGGCACTTTATGCTCCTGTTACAGTTCTCATCCAAAATATCGGCTTAGATTTTCATGATTCCATCTTGTATTAAAAAATTGGCTGTCCTTCACTGTGTAGTTAACAAACAAGTCCGGTAGAAAAAAAATATTTCAAGTATAACATGAAGATAGTTGTTTTTAATTGATATTGGTTTGATGTTAGTCACATTGACTTTTTTATCATTTAAATCATAGCATAGAAGGTCATTCATCAAAAACAATATCAAGTATTGGAGGGCTATAGCATTTACTTGGTTTACATGAATATGGATTTGCAGCTGTAGCAAGGGATTCTGTTCAGTGCCTTCCAAAATGTGATGCTACCAATAAGTTTGGAGCATCTTTTGAGACTACTAGGGGTCATCGTTCGGTAATGGAATCTCACTACTCGTTGCATCAACTTTGGCTATCCATCTTTAGTATATAATTTGGAGAACTAGTTTCTAAGTTCGCTTTGTAATTGTAGATTAGACCTGTTGCGTAAATTGAAATGATTTCGTGCATGAAACCAGACATTTGGGCCTCCTTGGCTGATGAAGTACCATCGTGGGTTTCAGATAAAAGGAATAAAACATCAGTGGATCGAACTGTGAAATGGCTGGATGAATGCATCACAATGAACTCAGTAAGAACGCTAGGATGCCCCTTTTATATATCAGCATCTTGTCTGGATTTGAACTCTGCTAGATGTGTAATGTTGAGAAATGTAGGTCAATGAGCATTATTTCTAACCATTTAGATAAGCAGTACATAATTGCTCTTTCGTAGTGTGTTGTGTGTTTTAAACTTCAACAAGTGGCTAGGCCCATAGAGAGAAAATAAGTTGGGAAAAGCAGCAGTCAAAAAAGCATCTGGTTGTGCATTTCTATAGGGAAATTTATAGCATCATAGTGCTTAGATAGGAAAATTCATGAACGAGCAGAAAGTAGGCCAAAATTTCCAGAGCAAGCTGACCAAAGGAGGGGAATTCTGGTCCCTTTTAAATTGATAGAAATGACATAATTCTATCAGATAAGAATGTTGTGAAAAAGTTTGGTGGATTTCTTTAAACACTTGTTCATGGTAGGTGATCATCAGAGATTTTCCAAAATCTAAATACTAGAGTGATTGATTGGACAGGCTTTTACTTGCATATTTTAGGTATAGATTCTCAAGTGTAAAAGGGTTAGGTGCCTAAAGTTCCCTAGACTAGGTGCCTATGGGTAACGATTGGATTGTGGTTTCCCACTAGTATTTTAGAAGTGGTTGTTAACAAATGCACAAATGAAACATTTTAATAGCTTTGTTGTTTCTGGGTATTATGGCATGAGATAGCTGTTCGGTGCTTCCAGAGTATCATTTTAATAGCTTTCAAAATGCCCTCAAATCAAGAGTATTGGGCCATATACATGCAGCAGAATTTATAGGTTGTATATAAATTTTTCTTATGAAGAGATATTCTTAAGATTTTTAGAGTGTATCTAAGTCATATGACTGTTGCCTTTTCTGATAAGAGTCATATGGCTGTTGTTGACTCATTAATGCAAAAGCAATATGGGAATGCTGTCATGACTCATGGTGTAGTGATAGTATTTAGTCTTTATGTGTAGGTTTGGTCCTTTTTATATTTTTTTCTTTTCCCAATCATGTCAAGAGTGATTTGAAACAAGTAACTTATTATTATAATGAAGACAGGTGGAGCTGTTTTTGGAGCTATTGTTGGAGGCAATGATGTAGAAGAACGTCAAGATGTGCTGCGGAGGTGGTTAAGCGTTATGTATCAGGTTCAATAACTTGTTCTTTGTATATAAGGTGAAACAGTGGTAATTAGTTCAAGAATTAATTGCTGTAGAGCTAATTTTGTGTTATATCAATTTCTAGCTTCTTGCAGAATTTGTCTAGACTATCAAAGTTCTTAATAGCCATTAACATAACAAATAAAATTTTGAACAGAATGTCAATATTTGTGTTCCTCGACCCTATCTAGCATGTAAGGAATGATTGTTTTCCGGTTGCATTTTGTTCAGTTAAATTGATCATTTCTATCCTAGTTTCCGGCCGCTTATTCTGATTTCTCAGTTTGAACAAACAATTTTTGGTAGGTTACTGGATTGGAGGTTTTGGGCTTGGAGAGAGCACGGATGAGCGTCCTGCCCTTCTTGATGCCATTACGGTAAGGATTTTGCTTGTAATGCTGTCAGGTTTGTCTTTTGGAACATTGCCATCCTTTTTCTGCACCAACTGAGAACGTGGATGTTTTGCATGTGAACCATAATAGTTGATGAGTTGAACTTTGAAGCTTAAGAGTAGCAAGTTGGATTTGTTTGTGTAGTCCTCTGTTAACAATCTGTCGGAGTTTGGTTTTTTTTCTTTTGAAGTTTTTTCTATTCTCTTTACGTTCATTGTCCTTGATGAATTTTTTTTCAAAGACAAATAAAAAGGCAACTTCACTGTGATTCTCATTACATCCATATCCCTTTGCTTCTCTCTCCTTCTCTGCGTCACTTTCCGTATCCTTTCTTTTCTATCTTCCCTCTCTGTATTCTAAGTAGCTCTATCCAAAGCTGTGTTGCCTCATGTCCACTTTTATTACACATTTTCCCGCATGTGTTTTGTAAGCGAAAAGGAAATGTACTACTTGCAATTCTTTCATTCCTTGCACAGGATGTTTTACCAGAGGAAATGCCCCGCATGACTGTGGCTTGGACTTCAGGTATAGTTTTTCATCTTTTAGATCAAGTTGGTTGCACCAACTTTATGTGTAACCTCAATTTGACCAAGAGTCATGAAGCATGCGTCTTGTTTTGACTTTTGGTTGATTTCGTCCACCACTGTGTGAAGCTGGAAATACATATTTTTAATATTTTATGGAACCTAAACATTTAAGGCTACGTTGGTTTATGGTTTTAGTAAGCCTAAATTTTTGTAGCTTGTGACTAAGTTTGTGTTTAATAATCATCACTATTAATGCAGACTTTATGATCCTTTTTGTAATATTATTGTGAGTTCTCTGGCCCCTAGAAGAGCATTTTGTAATTGTCGTTTGTCAATCAAACTAGTTTTGTCTAAATATCACTTTTGTTAATATATATATATATATATATATAGATAGACAGACAGACATATAAGGCAACCCCTCTGATAAAATGTTGGATGGCCCCTGACAAATACTGTTCAAATCACTTGCTAAATGTCTATGAAATGCTGGCTCAGATACTGCTGGAGTTGTATGAACATTTCACTACCTGAAATATACTTAGTTGTCTCAATCTTCATTCTCACAGTAATAATTCTCACCTTATTGCAGACATAACACTCATCATTATCTAGGATTCTTTCGATCAATCCGAGAAGCAATCAAGGCTGGAAAATTTCAGCAATTTCATCAGAAATTTGTTGAGGTGAGGCGCAATCATCTAGCCTTGGCAACATTGGCACTCGGCTCATGACGAGCTCGTCAAACAAGTAATTTTAATATTGAAAATAA

mRNA sequence

ATGAAATTCGCGGTGAAGGCATGGGGCAACGGCAAGGCGCGCACCGGATTTCTACAGCTCGTCCACTGCGCTTCCGCCATGGAGACTCCCGCGTTACTTCTCTCTACTCGCAAAGGACTGCCTAATTTCAGCTCTCCTGATCTTCTTCCCTCTCTCCCTTCGCCTGATTCTCACCTCCTCCAATTCAGTCCTCTTCACTTGGGAATATGTCATATACGGAAAAGGACGAAATTACTGGCGAATGGATGTAGAGCGTCTGTAGCAAGGGATTCTGTTCAGTGCCTTCCAAAATGTGATGCTACCAATAAGTTTGGAGCATCTTTTGAGACTACTAGGGGTCATCGTTCGACAGGTGGAGCTGTTTTTGGAGCTATTGTTGGAGGCAATGATGTAGAAGAACGTCAAGATGTGCTGCGGAGTTTGAACAAACAATTTTTGGTAGGTTACTGGATTGGAGGTTTTGGGCTTGGAGAGAGCACGGATGAGCGTCCTGCCCTTCTTGATGCCATTACGGTAAGGATTTTGCTTGTAATGCTGTCAGGATGTTTTACCAGAGGAAATGCCCCGCATGACTGTGGCTTGGACTTCAGACATAACACTCATCATTATCTAGGATTCTTTCGATCAATCCGAGAAGCAATCAAGGCTGGAAAATTTCAGCAATTTCATCAGAAATTTGTTGAGGTGAGGCGCAATCATCTAGCCTTGGCAACATTGGCACTCGGCTCATGACGAGCTCGTCAAACAAGTAATTTTAATATTGAAAATAA

Coding sequence (CDS)

ATGAAATTCGCGGTGAAGGCATGGGGCAACGGCAAGGCGCGCACCGGATTTCTACAGCTCGTCCACTGCGCTTCCGCCATGGAGACTCCCGCGTTACTTCTCTCTACTCGCAAAGGACTGCCTAATTTCAGCTCTCCTGATCTTCTTCCCTCTCTCCCTTCGCCTGATTCTCACCTCCTCCAATTCAGTCCTCTTCACTTGGGAATATGTCATATACGGAAAAGGACGAAATTACTGGCGAATGGATGTAGAGCGTCTGTAGCAAGGGATTCTGTTCAGTGCCTTCCAAAATGTGATGCTACCAATAAGTTTGGAGCATCTTTTGAGACTACTAGGGGTCATCGTTCGACAGGTGGAGCTGTTTTTGGAGCTATTGTTGGAGGCAATGATGTAGAAGAACGTCAAGATGTGCTGCGGAGTTTGAACAAACAATTTTTGGTAGGTTACTGGATTGGAGGTTTTGGGCTTGGAGAGAGCACGGATGAGCGTCCTGCCCTTCTTGATGCCATTACGGTAAGGATTTTGCTTGTAATGCTGTCAGGATGTTTTACCAGAGGAAATGCCCCGCATGACTGTGGCTTGGACTTCAGACATAACACTCATCATTATCTAGGATTCTTTCGATCAATCCGAGAAGCAATCAAGGCTGGAAAATTTCAGCAATTTCATCAGAAATTTGTTGAGGTGAGGCGCAATCATCTAGCCTTGGCAACATTGGCACTCGGCTCATGA

Protein sequence

MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLLQFSPLHLGICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGASFETTRGHRSTGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRILLVMLSGCFTRGNAPHDCGLDFRHNTHHYLGFFRSIREAIKAGKFQQFHQKFVEVRRNHLALATLALGS
Homology
BLAST of Carg24976 vs. NCBI nr
Match: KAG7015882.1 (hypothetical protein SDJN02_20985, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.1 bits (1289), Expect = 5.3e-138
Identity = 243/243 (100.00%), Postives = 243/243 (100.00%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHLGICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGASFETTRGHRSTGGA 120
           QFSPLHLGICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGASFETTRGHRSTGGA
Sbjct: 61  QFSPLHLGICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGASFETTRGHRSTGGA 120

Query: 121 VFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRILLVMLS 180
           VFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRILLVMLS
Sbjct: 121 VFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRILLVMLS 180

Query: 181 GCFTRGNAPHDCGLDFRHNTHHYLGFFRSIREAIKAGKFQQFHQKFVEVRRNHLALATLA 240
           GCFTRGNAPHDCGLDFRHNTHHYLGFFRSIREAIKAGKFQQFHQKFVEVRRNHLALATLA
Sbjct: 181 GCFTRGNAPHDCGLDFRHNTHHYLGFFRSIREAIKAGKFQQFHQKFVEVRRNHLALATLA 240

Query: 241 LGS 244
           LGS
Sbjct: 241 LGS 243

BLAST of Carg24976 vs. NCBI nr
Match: XP_038885042.1 (queuine tRNA-ribosyltransferase accessory subunit 2-like [Benincasa hispida])

HSP 1 Score: 260.4 bits (664), Expect = 1.6e-65
Identity = 175/401 (43.64%), Postives = 192/401 (47.88%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTGFLQL +C +A+ETPALLLSTRKGLPNF SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPNAIETPALLLSTRKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L      +VARDSVQCLP+CDATNKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISRIGGLQHLLG----LHEYGFVAVARDSVQCLPECDATNKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITL 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRI 240
            STGGAVFGAIVGGND+EERQ     + K+ + GYWIGGFG GES +ERPALLDA+T  +
Sbjct: 181 NSTGGAVFGAIVGGNDIEERQRCAVEVVKRNVSGYWIGGFGSGESMEERPALLDAVTDVL 240

Query: 241 ------------------------------------------LLVMLSGCFTRGNAPH-- 242
                                                     L   L+G   +  A H  
Sbjct: 241 PEEMPRMICGLGLPEEILQGIATGVDLFDSAYIYHLTLGGFALTFPLNGIVEKQPAAHLK 300

BLAST of Carg24976 vs. NCBI nr
Match: XP_022152510.1 (queuine tRNA-ribosyltransferase accessory subunit 2-like [Momordica charantia])

HSP 1 Score: 255.4 bits (651), Expect = 5.0e-64
Identity = 174/403 (43.18%), Postives = 192/403 (47.64%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTG LQL +C +A+ETPALLLSTRKGL NF SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNGKARTGVLQLGNCPTAIETPALLLSTRKGLLNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSV CLP+CDA NKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLG----LHEYGFAAVARDSVWCLPECDAANKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSLRPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITS 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAIT--- 240
            STGGAVFGAIVGGNDVEERQ     + K+ + GYWIGGFGLGES DERPALLDA+T   
Sbjct: 181 NSTGGAVFGAIVGGNDVEERQRCAAEVIKRNVSGYWIGGFGLGESMDERPALLDAVTGIL 240

Query: 241 ------------------------------------------------------------ 244
                                                                       
Sbjct: 241 PEEMPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTIGGFALTFPLNGIVEKVPDLHLN 300

BLAST of Carg24976 vs. NCBI nr
Match: XP_008441158.1 (PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like [Cucumis melo])

HSP 1 Score: 255.0 bits (650), Expect = 6.6e-64
Identity = 171/401 (42.64%), Postives = 189/401 (47.13%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTGFLQL +C +A++TPALLLST KGLPNF SPDLLPSLPSPDSH+L
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTSKGLPNFISPDLLPSLPSPDSHIL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSVQCLP+CDATNKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLG----LHEYGFAAVARDSVQCLPQCDATNKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITL 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRI 240
            STGGAVFGAIVGGND+EERQ     + K+ + GYWIGGFG GES DERP+LLDA+T  +
Sbjct: 181 NSTGGAVFGAIVGGNDLEERQRCAVEVVKRNVSGYWIGGFGFGESMDERPSLLDAVTDVL 240

Query: 241 ------------------------------------------LLVMLSGCFTRGNAPH-- 242
                                                     L   L G   +  A H  
Sbjct: 241 PEEMPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLDGIVEKQPAAHLI 300

BLAST of Carg24976 vs. NCBI nr
Match: XP_004138668.1 (queuine tRNA-ribosyltransferase accessory subunit 2 [Cucumis sativus] >KGN63275.2 hypothetical protein Csa_022389 [Cucumis sativus])

HSP 1 Score: 250.8 bits (639), Expect = 1.2e-62
Identity = 171/399 (42.86%), Postives = 193/399 (48.37%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTGFLQL +C +A++TPALLLSTRKGLP+F SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTRKGLPHFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------GICHIRKRTKLLA--NGCRASVARDSVQCLPKCDATNKFGASFETT 120
           QFSPLH         I +I     LL       A+VARDSVQ LP+CDATNKFGASFETT
Sbjct: 61  QFSPLHFLEGPSSKTISNIGGLQHLLGLHEYGFAAVARDSVQSLPECDATNKFGASFETT 120

Query: 121 RGHR-----------------------------------------------------STG 180
            G R                                                     STG
Sbjct: 121 CGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECIALNSTG 180

Query: 181 GAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAIT------- 240
           GAVFGAIVGGND+EERQ     + K+ + GYWIGGFG GES DERP+LLDA+T       
Sbjct: 181 GAVFGAIVGGNDLEERQRCAVEVVKRNVSGYWIGGFGFGESMDERPSLLDAVTDVLPEEM 240

Query: 241 ------------------------------------------------------------ 244
                                                                       
Sbjct: 241 PRMICGLGLPEEVLQGIAAGVDLFDSAYIYHLTLGGFALTFPLDGIVEKQPDAHLIDNAS 300

BLAST of Carg24976 vs. ExPASy TrEMBL
Match: A0A6J1DE50 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Momordica charantia OX=3673 GN=LOC111020219 PE=3 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 2.4e-64
Identity = 174/403 (43.18%), Postives = 192/403 (47.64%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTG LQL +C +A+ETPALLLSTRKGL NF SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNGKARTGVLQLGNCPTAIETPALLLSTRKGLLNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSV CLP+CDA NKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLG----LHEYGFAAVARDSVWCLPECDAANKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSLRPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITS 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAIT--- 240
            STGGAVFGAIVGGNDVEERQ     + K+ + GYWIGGFGLGES DERPALLDA+T   
Sbjct: 181 NSTGGAVFGAIVGGNDVEERQRCAAEVIKRNVSGYWIGGFGLGESMDERPALLDAVTGIL 240

Query: 241 ------------------------------------------------------------ 244
                                                                       
Sbjct: 241 PEEMPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTIGGFALTFPLNGIVEKVPDLHLN 300

BLAST of Carg24976 vs. ExPASy TrEMBL
Match: A0A1S3B3I7 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo OX=3656 GN=LOC103485379 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 3.2e-64
Identity = 171/401 (42.64%), Postives = 189/401 (47.13%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTGFLQL +C +A++TPALLLST KGLPNF SPDLLPSLPSPDSH+L
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTSKGLPNFISPDLLPSLPSPDSHIL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSVQCLP+CDATNKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLG----LHEYGFAAVARDSVQCLPQCDATNKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITL 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAITVRI 240
            STGGAVFGAIVGGND+EERQ     + K+ + GYWIGGFG GES DERP+LLDA+T  +
Sbjct: 181 NSTGGAVFGAIVGGNDLEERQRCAVEVVKRNVSGYWIGGFGFGESMDERPSLLDAVTDVL 240

Query: 241 ------------------------------------------LLVMLSGCFTRGNAPH-- 242
                                                     L   L G   +  A H  
Sbjct: 241 PEEMPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLDGIVEKQPAAHLI 300

BLAST of Carg24976 vs. ExPASy TrEMBL
Match: A0A6J1JNK0 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita maxima OX=3661 GN=LOC111487476 PE=3 SV=1)

HSP 1 Score: 249.6 bits (636), Expect = 1.3e-62
Identity = 170/403 (42.18%), Postives = 192/403 (47.64%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW N KARTG LQL +C +A++TPALLLST KGLPNF SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCLQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSVQCLP+C+A+NKFGAS
Sbjct: 61  QFSPLHFLEGPSLKTISSIGGLQHLLG----LHEYVFAAVARDSVQCLPECNASNKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 180
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIKPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECINL 180

Query: 181 -STGGAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAIT--- 240
            STGGA+FGAIVGGND+EERQ     + K+ + GYWIGGFGLGES DERPALLDA+T   
Sbjct: 181 NSTGGAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVL 240

Query: 241 ------------------------------------------------------------ 244
                                                                       
Sbjct: 241 PEELPRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLN 300

BLAST of Carg24976 vs. ExPASy TrEMBL
Match: A0A6J1HIG1 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita moschata OX=3662 GN=LOC111464336 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 6.6e-62
Identity = 169/397 (42.57%), Postives = 190/397 (47.86%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW N KARTG +QL +C +A++TPALLLST KGLPNF SPDLLPSLPSPDSHLL
Sbjct: 1   MKFAVKAWSNCKARTGCIQLGNCPTAIDTPALLLSTCKGLPNFISPDLLPSLPSPDSHLL 60

Query: 61  QFSPLHL-------GICHIRKRTKLLA--NGCRASVARDSVQCLPKCDATNKFGASFETT 120
           QFSPLH         I  I     LL       A+VARDSVQCLP+C+A+NKFGASFETT
Sbjct: 61  QFSPLHFLEGPSLKAISSIGGLQHLLGLHEYVFAAVARDSVQCLPECNASNKFGASFETT 120

Query: 121 RGHR-----------------------------------------------------STG 180
            G R                                                     STG
Sbjct: 121 CGRRLIRPVEYVELISSMRPDIWASLADEVPSWVSDKRNKTSVDRTVRWLDECIKLNSTG 180

Query: 181 GAVFGAIVGGNDVEERQDVLRSLNKQFLVGYWIGGFGLGESTDERPALLDAIT------- 240
           GA+FGAIVGGND+EERQ     + K+ + GYWIGGFGLGES DERPALLDA+T       
Sbjct: 181 GAIFGAIVGGNDMEERQRCALEVVKRNVSGYWIGGFGLGESIDERPALLDAVTDVLPEEL 240

Query: 241 ------------------------------------------------------------ 242
                                                                       
Sbjct: 241 PRMICGLGLPEEILQGIAAGVDLFDSAYIYHLTLGGFALTFPLNGIAEKRPDTHLNDDAS 300

BLAST of Carg24976 vs. ExPASy TrEMBL
Match: A0A5A7T128 (Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00430 PE=3 SV=1)

HSP 1 Score: 217.2 bits (552), Expect = 7.4e-53
Identity = 126/237 (53.16%), Postives = 141/237 (59.49%), Query Frame = 0

Query: 1   MKFAVKAWGNGKARTGFLQLVHCASAMETPALLLSTRKGLPNFSSPDLLPSLPSPDSHLL 60
           MKFAVKAW NGKARTGFLQL +C +A++TPALLLST KGLPNF SPDLLPSLPSPDSH+L
Sbjct: 1   MKFAVKAWSNGKARTGFLQLGNCPAAIDTPALLLSTSKGLPNFISPDLLPSLPSPDSHIL 60

Query: 61  QFSPLHL-------------GICHIRKRTKLLANGCRASVARDSVQCLPKCDATNKFGAS 120
           QFSPLH              G+ H+      L     A+VARDSVQCLP+CDATNKFGAS
Sbjct: 61  QFSPLHFLEGPSSKTISSIGGLQHLLG----LHEYGFAAVARDSVQCLPQCDATNKFGAS 120

Query: 121 FETTRGHR---------------------------------------------------- 172
           FETT G R                                                    
Sbjct: 121 FETTCGRRLIRPVEYIEMISSMKPDIWASLADEVPSWVSDKRNKTSVDRTVKWLDECITL 180

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7015882.15.3e-138100.00hypothetical protein SDJN02_20985, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_038885042.11.6e-6543.64queuine tRNA-ribosyltransferase accessory subunit 2-like [Benincasa hispida][more]
XP_022152510.15.0e-6443.18queuine tRNA-ribosyltransferase accessory subunit 2-like [Momordica charantia][more]
XP_008441158.16.6e-6442.64PREDICTED: queuine tRNA-ribosyltransferase subunit qtrtd1-like [Cucumis melo][more]
XP_004138668.11.2e-6242.86queuine tRNA-ribosyltransferase accessory subunit 2 [Cucumis sativus] >KGN63275.... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DE502.4e-6443.18Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Momordica charantia OX=36... [more]
A0A1S3B3I73.2e-6442.64Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo OX=3656 GN=L... [more]
A0A6J1JNK01.3e-6242.18Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1HIG16.6e-6242.57Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucurbita moschata OX=366... [more]
A0A5A7T1287.4e-5353.16Queuine tRNA-ribosyltransferase accessory subunit 2 OS=Cucumis melo var. makuwa ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002616tRNA-guanine(15) transglycosylase-likePFAMPF01702TGTcoord: 112..182
e-value: 7.7E-7
score: 28.6
coord: 197..230
e-value: 4.1E-8
score: 32.8
IPR036511Queuine tRNA-ribosyltransferase-likeGENE3D3.20.20.105coord: 74..181
e-value: 1.6E-8
score: 35.7
IPR036511Queuine tRNA-ribosyltransferase-likeGENE3D3.20.20.105coord: 182..240
e-value: 6.2E-8
score: 33.7
IPR036511Queuine tRNA-ribosyltransferase-likeSUPERFAMILY51713tRNA-guanine transglycosylasecoord: 1..229
NoneNo IPR availablePANTHERPTHR46064QUEUINE TRNA-RIBOSYLTRANSFERASE ACCESSORY SUBUNIT 2coord: 198..234
coord: 118..172
coord: 1..116

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg24976-RACarg24976-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006400 tRNA modification
molecular_function GO:0016763 pentosyltransferase activity